1,371 results match your criteria sepharose cl-6b


Restriction endonuclease T.Smu451I with new cleavage specificity-neoschizomer of T.AsuI.

Folia Microbiol (Praha) 2021 May 5. Epub 2021 May 5.

Korean Center for Culture Collection, Pyongyang, Democratic People's Republic of Korea.

A specific type II restriction endonuclease T.Smu451I has been purified to electrophoretic homogeneity from the frozen cells of soil bacterium Sphingobacterium multivorum 451 (formerly Flavobacterium multivorum 451), using ultrasonic grinding, nucleic acid removal by streptomycin sulfate, protein precipitation by ammonium sulfate and phosphocellulose P-11, DEAE-Cellulose DE-52, Hepharin-Sepharose CL-6B chromatography, and elucidated several characteristics of T.Smu451I. Read More

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Kirkiin: A New Toxic Type 2 Ribosome-Inactivating Protein from the Caudex of .

Toxins (Basel) 2021 Jan 22;13(2). Epub 2021 Jan 22.

Department of Experimental, Diagnostic and Specialty Medicine-DIMES, General Pathology Section, Alma Mater Studiorum-University of Bologna, 40126 Bologna, Italy.

Ribosome-inactivating proteins (RIPs) are plant toxins that irreversibly damage ribosomes and other substrates, thus causing cell death. RIPs are classified in type 1 RIPs, single-chain enzymatic proteins, and type 2 RIPs, consisting of active A chains, similar to type 1 RIPs, linked to lectin B chains, which enable the rapid internalization of the toxin into the cell. For this reason, many type 2 RIPs are very cytotoxic, ricin, volkensin and stenodactylin being the most toxic ones. Read More

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January 2021

Extraction optimization, physicochemical properties and antioxidant and hypoglycemic activities of polysaccharides from roxburgh rose (Rosa roxburghii Tratt.) leaves.

Int J Biol Macromol 2020 Dec 28;165(Pt A):517-529. Epub 2020 Sep 28.

College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China; Jilin Provincial Key Lab of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address:

The optimum extraction conditions of polysaccharides from roxburgh rose (Rosa roxburghii Tratt.) leaves (RLP) were obtained by response surface methodology (RSM), which were a liquid to solid ratio of 21.16 mL/g, an extraction temperature of 81. Read More

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December 2020

Immunomodulatory effect of polysaccharides from the extraction of (Blume) Hook. f. et Thomson (Campanulaceae) roots in female rats.

Nat Prod Res 2020 Aug 3:1-5. Epub 2020 Aug 3.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medcial University, Guiyang, PR China.

In this study, the crude polysaccharides of (CJP) was obtained from (Blume) Hook. f. et Thomson using hot water extraction method, which was separated and purified by DEAE-cellulose column and Sepharose CL-6B column. Read More

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Novel thermostable lipase produced by a thermo-halophilic bacterium that catalyses hydrolytic and transesterification reactions.

Heliyon 2020 Jul 24;6(7):e04520. Epub 2020 Jul 24.

Biomolecules Application Research Group, Chemistry Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, Indonesia.

Lipase belongs to the class of hydrolytic enzymes that are widely used in the biotechnology industries. The goal of this research was to purify and characterize lipase produced from a thermo-halophilic bacterium, namely Pria Laot Sabang 80 (PLS 80). Purification was performed using ammonium sulphate fractionation, followed by gel filtration chromatography using Sepharose Cl-6B. Read More

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Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.

An Acad Bras Cienc 2020 27;92 Suppl 2:e20180838. Epub 2020 Jul 27.

School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, China.

In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated. It was observed that Tween 40 and ethanol displayed a stimulatory effect on EPS secretion. The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22. Read More

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September 2020

Structural characterization and immunomodulatory activity of a polysaccharide from Eurotium cristatum.

Int J Biol Macromol 2020 Nov 13;162:609-617. Epub 2020 Jun 13.

Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Biotech & Biomed Research Institute, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China. Electronic address:

By combining DEAE-cellulose-52 and Sepharose-CL-6B chromatography, a heteropolysaccharide ECIP-1A with an average molecular weight of 24,336 Da was isolated from the hyphae of Eurotium cristatum. The main components of the monosaccharides of ECIP-1A were mannose, glucose and galactose, and their molar ratio is 4.003: 0. Read More

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November 2020

Characterization and comparison of two peptide-tag specific nanobodies for immunoaffinity chromatography.

J Chromatogr A 2020 Aug 25;1624:461227. Epub 2020 May 25.

Liaoning Key Laboratory of Molecular Recognition and Imaging, School of Bioengineering, Dalian University of Technology, No. 2 Linggong Road, Dalian 116023, PR China. Electronic address:

Affinity chromatography is generally regarded as a powerful tool allowing the single step purification of recombinant proteins with high purity and yields. However, for most protein products, affinity purification methods for industrial applications are not readily available, mainly due to the lack of specific and robust natural counterparts that could function as affinity ligands. In this study, we explored the applicability of nanobody-based peptide-tag immunorecognition systems as a platform for affinity chromatography. Read More

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Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.

Int J Biol Macromol 2020 Sep 11;159:455-460. Epub 2020 May 11.

Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, China; School of Laboratory Medicine, Zunyi Medical University, Zunyi 563006, China. Electronic address:

Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear. In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column. Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan. Read More

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September 2020

Bioprocess development for L-asparaginase production by Streptomyces rochei, purification and in-vitro efficacy against various human carcinoma cell lines.

Sci Rep 2020 05 14;10(1):7942. Epub 2020 May 14.

Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt.

In the near future, the demand for L-asparaginase is expected to rise several times due to an increase in its clinical and industrial applications in various industrial sectors, such as food processing. Streptomyces sp. strain NEAE-K is potent L-asparaginase producer, isolated and identified as new subsp. Read More

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Isolation and purification of acidic polysaccharides from Agaricus blazei Murill and evaluation of their lipid-lowering mechanism.

Int J Biol Macromol 2020 Aug 25;157:276-287. Epub 2020 Apr 25.

College of Pharmacy, Beihua University, Jilin 132013, China. Electronic address:

Polysaccharides are important active constituents of Agaricus blazei Morrill. In the present study, WABM-A was isolated from WABM using DEAE-cellulose, and subsequently purified using sepharose CL-6B to obtain the acidic polysaccharide WABM-A-b. WABM-A-b is mainly composed of Glc dextran, with a molecular weight of 10 KDa and β-1,6-D-Glcp as its main chain. Read More

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Purification and Assays of Tachylectin-2.

Methods Mol Biol 2020 ;2132:309-316

Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.

Tachylectin-2, a 27-kDa protein consisting of a five-bladed β-propeller structure, is purified by three steps of chromatography, including dextran sulfate-Sepharose CL-6B, CM-Sepharose CL-6B, and Mono S. Three isolectins of tachylectin-2 including tachylectin-2a, -2b, and -2c are purified. These isolectins exhibit hemagglutinating activity against human A-type erythrocytes in a Ca-independent manner with tachylectin-2b showing the highest activity. Read More

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Characterization of carbaryl-degrading strain Bacillus licheniformis B-1 and its hydrolase identification.

Biodegradation 2020 04 18;31(1-2):139-152. Epub 2020 Apr 18.

College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, 625014, People's Republic of China.

Pesticides introduced inadvertently or deliberately into environment by anthropogenic activity have caused growing global public concern, therefore the search of approaches for elimination of such xenobiotics should be encouraged. A cypermethrin-degrading bacterial strain Bacillus licheniformis B-1 was found to efficiently degrade carbaryl in LB medium at concentrations of 50-300 mg L within 48 h, during which temperature and pH played important roles as reflected by increase in pollutant depletion. A stimulatory effect of Fe and Mn on microbial growth was observed, whereas Cu caused inhibition of degradation. Read More

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Study of imidazole performance as pseudo-affinity ligand in the purification of IgG from bovine milk.

Anal Biochem 2020 05 19;597:113693. Epub 2020 Mar 19.

Department of Organic Chemistry and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.

The spherical sepharose CL-6B beads were activated by epichlorohydrin in different epoxy contents (80, 120 and 160 μmol/mL) and, l-histidine and imidazole as pseudo-affinity ligands were covalently immobilized to them. Some linkers with different length, (1,2-ethanediol diglycidyl ether and 1,4-butanediol diglycidyl ether) were synthesized for activation of sepharose and the activated sepharose beads modified with imidazole and the performance of these adsorbents in the purification of immunoglobulin G from bovine milk were evaluated. Among the l-histidine bearing adsorbents, higher adsorption of IgG (0. Read More

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Evaluating the ion exchange chromatography for matrix-assisted PEGylation and purification of consensus interferon.

Biotechnol Appl Biochem 2020 Mar 3;67(2):196-205. Epub 2019 Nov 3.

National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.

Scientists have implemented protein-PEGylation technology for boosting-up the pharmacokinetics and stability of recombinant therapeutic proteins. In the present study, (a) matrix-assisted PEGylation was compared with solution-phase PEGylation and (b) matrix-assisted PEGylation was performed with different ion exchange resins for impact of chromatography medium on yield and purity of PEGylated product. DEAE Sepharose CL 6B, DEAE Fracto gel, and Macro cap Q ion exchange chromatography medium were compared for on column PEGylation and purification of cIFN. Read More

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Synthesis and characterization of crustin capped titanium dioxide nanoparticles: Photocatalytic, antibacterial, antifungal and insecticidal activities.

J Photochem Photobiol B 2019 Oct 3;199:111620. Epub 2019 Sep 3.

Biomaterials and Biotechnology in Animal Health Lab, Nanobiosciences and Nanopharmacology Division, Department of Animal Health and Management, Alagappa University, Karaikudi 630004, Tamil Nadu, India. Electronic address:

Current scenario of bio-nanotechnology, successfully fabrication of ultrafine titanium dioxide nanoparticles (TiONPs) using various biological protein sources for the multipurpose targets. The present research report involves synthesis of TiONPs using antimicrobial peptide (AMP) crustin (Cr). Crustin previously purified from the blue crab, Portunus pelagicus haemolymph, by blue Sepharose CL-6B matrix assisted affinity column chromatography. Read More

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October 2019

Optimization of ultrasonic-assisted enzymatic extraction of polysaccharides from thick-shell mussel (Mytilus coruscus) and their antioxidant activities.

Int J Biol Macromol 2019 Nov 16;140:1116-1125. Epub 2019 Aug 16.

Zhejiang Marine Development Research Institute, Zhoushan, China.

This study aimed to obtain the purified fractions of Mytilus coruscus polysaccharides (MCPs) and investigate their antioxidant activities. MCPs were prepared through ultrasonic-assisted enzymatic extraction optimized by employing the response surface methodology. A single-factor experiment was conducted using the Box-Behnken design to determine the optimum extraction conditions of MCPs. Read More

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November 2019

Protective activity of beta-1, 3-glucan binding protein against AAPH induced oxidative stress in Saccharomyces cerevisiae.

Int J Biol Macromol 2019 Oct 22;138:890-902. Epub 2019 Jul 22.

Crustacean Molecular Biology and Genomics Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block, 6(th) Floor, Burma Colony, Karaikudi 630004,Tamil Nadu, India. Electronic address:

The present work aimed to evaluate the antioxidant efficacy of beta-1,3-glucan binding/recognition protein against oxidative stress-induced Saccharomyces cerevisiae. Beta-1,3-glucan binding/recognition protein was attained from the Paratelphusa hydrodromus (Phβ-GBP) using laminarin coupled Sepharose CL-6B column. The structural characteristics of Phβ-GBP were analyzed through circular dichroism (CD), fourier transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance spectroscopy (H NMR) analysis. Read More

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October 2019

Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.

Int J Biol Macromol 2019 Sep 17;136:579-585. Epub 2019 Jun 17.

School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, PR China.

In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B. Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways. Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal. Read More

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September 2019

Bci528I, a new isoschizomer of EcoRI isolated from Bacillus circulans 528.

Folia Microbiol (Praha) 2019 Nov 1;64(6):803-808. Epub 2019 Apr 1.

Korean Center for Culture Collection, Pyongyang, Democratic People's Republic of Korea.

Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of Bci528I was 29,400 U/mg·protein. Read More

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November 2019

Isolation of a putative virulence agent, cytotoxic serine-elastase, from a newly isolated ZuhP13.

J Biosci 2019 Mar;44(1)

Department of Microbiology and Botany, Faculty of Science, Zagazig University, Zagazig 44519, Egypt,

A 48 kDa ZuhP13 elastase from P. aeruginosa isolated from a urine sample was successfully purified to 8.8-fold and 39% recovery by DEAE-Sepharose CL-6B and Sephadex G-100 chromatography. Read More

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Exopolysaccharides produced by yogurt-texture improving Lactobacillus plantarum RS20D and the immunoregulatory activity.

Int J Biol Macromol 2019 Jan 1;121:342-349. Epub 2018 Oct 1.

College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China. Electronic address:

The strain RS20D capable of significantly improving yogurt texture was isolated from traditional fermented vegetable products, and identified as Lactobacillus plantarum RS20D. The total exopolysaccharides (EPS) were prepared from reconstituted skim milk fermentation by RS20D, and purified through DEAE-Sepharose CL-6B and Sephadex G-100, and consequently the purified fraction designated as RS-r2 was obtained. The further work aimed to elucidate the structural features of RS-r2 via FT-IR spectrum, HPSEC and monosaccharide composition analysis was carried out. Read More

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January 2019

Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115.

BMC Pharmacol Toxicol 2018 Aug 23;19(1):51. Epub 2018 Aug 23.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.

Background: L-asparaginase is a potential therapeutic enzyme widely used in the chemotherapy protocols of pediatric and adult patients with acute lymphoblastic leukemia. However, its use has been limited by a high rate of hypersensitivity in the long-term used. Hence, there is a continuing need to search for other L-asparaginase sources capable of producing an enzyme with less adverse effects. Read More

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Production and characterization of a thermostable antifungal chitinase secreted by the filamentous fungus under submerged fermentation.

3 Biotech 2018 Aug 10;8(8):369. Epub 2018 Aug 10.

1Instituto de Química, Universidade Estadual Paulista, Rua Prof. Francisco Degni, 55, Quitandinha, Araraquara, SP 14800-900 Brazil.

The filamentous fungus produced extracellular antifungal chitinase when cultured under submerged fermentation (SbmF) using crab shells as the carbon source. Maximal chitinase production was achieved at 192 h of cultivation using minimal medium containing 1% chitin. The enzyme was purified 1. Read More

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Searching for crab-borne antimicrobial peptides: Crustin from Portunus pelagicus triggers biofilm inhibition and immune responses of Artemia salina against GFP tagged Vibrio parahaemolyticus Dahv2.

Mol Immunol 2018 09 30;101:396-408. Epub 2018 Jul 30.

Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India; Department of Zoology, Government College for Women, Kumbakonam, 612 001, Tamil Nadu, India.

Marine organisms represent a huge source of novel compounds for the development of effective antimicrobial drugs. The present study focus on the purification of the antimicrobial peptide crustin from the haemolymph of the blue swimmer crab, Portunus pelagicus, by blue Sepharose CL-6B matrix assisted affinity column chromatography. Crustin showed a single band with a molecular mass of 17 kDa in SDS-PAGE analysis. Read More

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September 2018

Effects of bed compression on protein separation on gel filtration chromatography at bench and pilot scale.

J Chem Technol Biotechnol 2018 Jul 9;93(7):1959-1965. Epub 2017 Oct 9.

Ipsen Limited Wrexham UK.

Background: Poorly packed chromatography columns are known to reduce drastically the column efficiency and produce broader peaks. Controlled bed compression has been suggested to be a useful approach for solving this problem. Here the relationship between column efficiency and resolution of protein separation are examined when preparative chromatography media were compressed using mechanical and hydrodynamic methods. Read More

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Characterization and Antioxidant Activity of the Exopolysaccharide Produced by GSBa-1.

J Microbiol Biotechnol 2018 Aug;28(8):1282-1292

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, P.R. China.

The exopolysaccharide (EPS) produced by GSBa-1 was isolated and purified by ethanol precipitation, and DEAE-cellulose and Sepharose CL-6B chromatographies. The molecular mass of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predominantly glucose, and it was further confirmed by NMR spectroscopy to be α-glucan that consisted of a trisaccharide repeating unit with possible presence of two α-(1→3) and one α-(1→6) glucosidic linkages. Read More

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Characterization and Antioxidant Activity of an Exopolysaccharide Produced by Rigidoporus microporus (Agaricomycetes).

Int J Med Mushrooms 2018 ;20(4):311-320

College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, China; Collaborative Innovation Center of Food Production and Safety, Zhengzhou, Henan, China.

In this investigation we developed an optimal fermentation medium to produce an exopolysaccharide (EPS) with antioxidant activity from Rigidoporus microporus. Lactose and tryptone were chosen as the optimal carbon and nitrogen sources, respectively, for EPS production, with a 6-day cultivation cycle. After removing proteins through the use of the Sevag method, one EPS fraction was purified from the culture filtrates by gel filtration chromatography on a Sepharose CL-6B column. Read More

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November 2018

Rhizoctonia solani fucomannogalactan: Chemical characterization and antiproliferative activity.

Int J Biol Macromol 2018 Aug 10;115:106-113. Epub 2018 Apr 10.

São Paulo State University (UNESP), Department of Microbiology and Immunology, Institute of Biosciences, Botucatu 18618-689, Brazil.

A fucomannogalactan from Rhizoctonia solani biomass was obtained after hot aqueous extraction and purified by freeze-thaw cycles and gel filtration chromatography on Sepharose CL-6B. The polysaccharide was homogeneous after HPSEC/RID analysis (M/M~1.1), displaying an average molecular weight of 15. Read More

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Optimization of ultrasound-assisted extraction of okra (Abelmoschus esculentus (L.) Moench) polysaccharides based on response surface methodology and antioxidant activity.

Int J Biol Macromol 2018 Jul 4;114:1056-1063. Epub 2018 Apr 4.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Beijing 100083, China. Electronic address:

This study determined the optimal conditions for ultrasound-assisted extraction of a water-soluble polysaccharide, Raw Okra Polysaccharide, from the fruit of okra using response surface methodology. The optimal extraction temperature, extraction time and ultrasonic power were 59°C, 30min and 522W, respectively, giving a yield of 10.35±0. Read More

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