150,213 results match your criteria separation method

Method performance verification for the combined detection and quantitation of the marine neurotoxins cyclic imines and brevetoxin shellfish metabolites in mussels (Mytilus edulis) and oysters (Crassostrea gigas) by UHPLC-MS/MS.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Jul 26;1179:122864. Epub 2021 Jul 26.

Centre for Environment Fisheries and Aquaculture Science (Cefas), Barrack Road, Weymouth, Dorset, DT4 8UB, United Kingdom. Electronic address:

A single laboratory method performance verification is reported for a rapid sensitive UHPLC-MS/MS method for the quantification of eight cyclic imine and two brevetoxin analogues in two bivalve shellfish matrices: mussel (Mytilus edulis) and Pacific oyster (Crassostrea gigas). Targeted cyclic imine analogues were from the spirolide, gymnodimine and pinnatoxin groups, namely 20-Me-SPX-C, 13-desMe-SPX-C, 13,19-didesMe-SPX-C, GYM-A, 12-Me-GYM, PnTx-E, PnTx-F and PnTx-G. Brevetoxin analogues consisted of the shellfish metabolites BTX-B5 and S-desoxy-BTX-B2. Read More

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Ti/TiO nanotubes sensitized PbS quantum dots as photoelectrodes applied for decomposition of anticancer drugs under simulated solar energy.

J Hazard Mater 2021 Jul 30;421:126751. Epub 2021 Jul 30.

Department of Environmental Technology, Faculty of Chemistry, University of Gdansk, 80-308 Gdansk, Poland. Electronic address:

One of the challenges in research into photoelectrocatalytic (PEC) degradation of pollutants is finding the appropriate photoanode material, which has a significant impact on the process efficiency. Among all others, photoelectrodes based on an ordered TiO nanotube arrays are a promising material due to well-developed surface area and efficient charge separation. To increase the PEC activity of this material, the SILAR method was used to decorate Ti/TiO nanotubes by PbS quantum dots (QD). Read More

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Cyclodextrin-based dispersive liquid-liquid microextraction for the determination of fungicides in water, juice, and vinegar samples via HPLC.

Food Chem 2021 Jul 22;367:130664. Epub 2021 Jul 22.

College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China; Graduate Education Innovation Center on Baijiu Bioengineering in Shanxi Province, Taigu, Shanxi 030801, PR China. Electronic address:

Cyclodextrin-based dispersive liquid-liquid microextraction (CD-DLLME) was developed for the determination of triazole and strobilurin fungicides in water, juice, and vinegar samples using high-performance liquid chromatography-diode-array detection (HPLC-DAD). Undecanol, which is a green solvent, was selected as the extraction solvent. A cyclodextrin aqueous solution was chosen as the dispersion solvent and demulsifier to avoid the use of a toxic dispersion solvent and eliminate the centrifugation step. Read More

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Multiple dimensions underlying the functional organisation of the language network.

Neuroimage 2021 Jul 31:118444. Epub 2021 Jul 31.

MRC Cognition and Brain Sciences Unit, University of Cambridge, UK. Electronic address:

Understanding the different neural networks that support human language is an ongoing challenge for cognitive neuroscience. Which divisions are capable of distinguishing the functional significance of regions across the language network? A key separation between semantic cognition and phonological processing was highlighted in early meta-analyses, yet these seminal works did not formally test this proposition. Moreover, organisation by domain is not the only possibility. Read More

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Formation of Multi-Channel Collagen Gels Investigated Using Particle Tracking Microrheology.

Biomacromolecules 2021 Aug 3. Epub 2021 Aug 3.

Faculty of Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Fukuoka 819-0395, Japan.

Collagen is one of the most common materials used to form scaffolds for tissue engineering applications. The multi-channel collagen gel (MCCG) obtained by the dialysis of an acidic collagen solution in a neutral buffer solution has a unique structure, with many capillaries of diameters several tens to a few hundred micrometers, and could be a potential candidate as a biomimetic scaffold for three-dimensional tissue engineering. In the present study, the formation of MCCG was investigated by rheological measurements based on a particle tracking method (particle tracking microrheology, PTM). Read More

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PRISM: A Novel Human Islet Isolation Technique.

Transplantation 2021 Jul 22. Epub 2021 Jul 22.

Dept of Internal Medicine, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands.

Background: Successful pancreatic islet isolations are a key requirement for islet transplantation in selected patients with type 1 diabetes. However, islet isolation is a technically complex, time-consuming, manual process. Optimization and simplification of the islet isolation procedure could increase islet yield and quality, require fewer operators and thus reduce cost. Read More

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Predicting Micropollutant Removal by Reverse Osmosis and Nanofiltration Membranes: Is Machine Learning Viable?

Environ Sci Technol 2021 Aug 3. Epub 2021 Aug 3.

Department of Civil and Environmental Engineering, Colorado State University, Fort Collins, Colorado 80523, United States.

Predictive models for micropollutant removal by membrane separation are highly desirable for the design and selection of appropriate membranes. While machine learning (ML) models have been applied for such purposes, their reliability might be compromised by data leakage due to inappropriate data splitting. More importantly, whether ML models can truly understand the mechanisms of membrane separation has not been revealed. Read More

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A DNAzyme-Based Dual-Stimuli Responsive Electrochemiluminescence Resonance Energy Transfer Platform for Ultrasensitive Anatoxin-a Detection.

Anal Chem 2021 Aug 3. Epub 2021 Aug 3.

Key Laboratory of Chem-Biosensing and Key Laboratory of Functional Molecular Solids of Anhui Province, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, Anhui 241000, China.

An effective and precise electrochemiluminescence resonance energy transfer (ECL-RET), including the efficient regulation over the proximity of a donor and an acceptor and the reliable stimuli responsive as well as the avoidance of undesirable probes leakage, etc., is significant for the development of an accurate and sensitive ECL detection method; yet, the current literature in documentation involves only a limited range of such ECL-RET systems. Herein, we propose an ECL-RET strategy with dually quenched ultralow background signals and a dual-stimuli responsive, accurate signal output for the ultrasensitive and reliable detection of anatoxin-a (ATX-a). Read More

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Cytoplasmic factories for axonemal dynein assembly.

Stephen M King

J Cell Sci 2021 Aug 3;134(15). Epub 2021 Aug 3.

Department of Molecular Biology and Biophysics, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030-3305, USA.

Axonemal dyneins power the beating of motile cilia and flagella. These massive multimeric motor complexes are assembled in the cytoplasm, and subsequently trafficked to cilia and incorporated into the axonemal superstructure. Numerous cytoplasmic factors are required for the dynein assembly process, and, in mammals, defects lead to primary ciliary dyskinesia, which results in infertility, bronchial problems and failure to set up the left-right body axis correctly. Read More

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A review of child sexual abuse cases presenting to a paediatric emergency department.

Ann Acad Med Singap 2021 Jul;50(7):527-535

Department of Emergency Medicine, KK Women's and Children's Hospital, Singapore.

Introduction: Child sexual abuse (CSA) adversely affects a child's growth and well-being. This study aimed to describe the profile of children presenting to a tertiary paediatric emergency department(ED) with CSA.

Methods: Children 0-16 years old presenting to KK Women's and Children's Hospital ED from June 2016 to August 2020 with sexual abuse were retrospectively reviewed. Read More

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Active binary switching of soft colloids: stability and structural properties.

Soft Matter 2021 Aug 3. Epub 2021 Aug 3.

Departamento de Física Aplicada, Universidad de Granada, Campus Fuentenueva S/N, 18071 Granada, Spain and Carlos I Institute of Theoretical and Computational Physics, Facultad de Ciencias, Universidad de Granada, Campus Fuentenueva S/N, 18071 Granada, Spain.

We employ reactive dynamical density functional theory (R-DDFT) and reactive Brownian dynamics (R-BD) simulations to study the non-equilibrium structure and phase behavior of an active dispersion of soft Gaussian colloids with binary interaction switching, i.e., we consider a one-component colloidal system in which every particle can individually switch stochastically between two interaction states (here, sizes 'big' and 'small') at predefined rates. Read More

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Yet another artefact rejection study: an exploration of cleaning methods for biological and neuromodulatory noise.

J Neural Eng 2021 Aug 2;18(4). Epub 2021 Aug 2.

Rehab Technologies, Istituto Italiano di Tecnologia, 16163 Genoa, Italy.

. Electroencephalography (EEG) cleaning has been a longstanding issue in the research community. In recent times, huge leaps have been made in the field, resulting in very promising techniques to address the issue. Read More

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Role of direct and indirect social and spatial ties in the diffusion of HIV and HCV among people who inject drugs: A cross-sectional community-based network analysis in New Delhi, India.

Elife 2021 Aug 3;10. Epub 2021 Aug 3.

Johns Hopkins University School of Medicine, Baltimore, United States.

People who inject drugs (PWID) account for some of the most explosive HIV and hepatitis C virus (HCV) epidemics globally. While individual drivers of infection are well understood, less is known about network factors, with minimal data beyond direct ties.

2,512 PWID in New Delhi, India were recruited in 2017-19 using a sociometric network design. Read More

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Separation and characterization of phenylamides from Piper kadsura using preparative supercritical fluid chromatography and ultra-high performance supercritical fluid chromatography-tandem mass spectrometry.

J Sep Sci 2021 Aug 3. Epub 2021 Aug 3.

Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, PR China.

A preparative supercritical fluid chromatography method for the separation of Piper kadsura obtained five phenylamide compounds, which had the same structural skeleton, but changed in the number and position of methoxyl substituents. To improve the separation selectivity of these structural analogues, silica, phenyl, and chiral stationary phases were screened. Only through the combination of Chiral C and phenyl columns could the separation of the five phenylamides be solved. Read More

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Medial meniscal ramp lesions in ACL-injured elite athletes are strongly associated with medial collateral ligament injuries and medial tibial bone bruising on MRI.

Knee Surg Sports Traumatol Arthrosc 2021 Aug 3. Epub 2021 Aug 3.

Fortius Clinic, 17 Fitzhardinge St, London, W1H 6EQ, UK.

Purpose: Medial menisco-capsular separations (ramp lesions) are typically found in association with anterior cruciate ligament (ACL) deficiency. They are frequently missed preoperatively due to low MRI sensitivity. The purpose of this article was to describe demographic and anatomical risk factors for ramp lesions, and to identify concomitant lesions and define their characteristics to improve diagnosis of ramp lesions on MRI. Read More

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A NiS co-catalyst decorated ZnInS/g-CN type-II ball-flower-like nanosphere heterojunction for efficient photocatalytic hydrogen production.

Dalton Trans 2021 Aug 3. Epub 2021 Aug 3.

College of Energy and Mechanical Engineering, Shanghai University of Electric Power, Shanghai, China. and Shanghai Engineering Research Center of Power Generation Environment Protection, Shanghai, China.

Promoting the separation of photogenerated electron-hole pairs and enhancing the charge carrier transfer are critical in photocatalysis. In our work, a ball-flower-like NiS/Zn3In2S6/g-C3N4 photocatalyst fabricated by a hydrothermal method exhibited superior performance for photocatalytic water splitting. The optimized 2. Read More

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Vertebral bone marrow T2* mapping using chemical shift encoding-based water-fat separation in the quantitative analysis of lumbar osteoporosis and osteoporotic fractures.

Quant Imaging Med Surg 2021 Aug;11(8):3715-3725

Department of Radiology, Klinikum Rechts der Isar, School of Medicine, Technical University of Munich, Munich, Germany.

Background: Chemical shift encoding-based water-fat separation techniques have been used for fat quantification [proton density fat fraction (PDFF)], but they also enable the assessment of bone marrow T2*, which has previously been reported to be a potential biomarker for osteoporosis and may give insight into the cause of vertebral fractures (i.e., osteoporotic traumatic) and the microstructure of the bone when applied to vertebral bone marrow. Read More

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Magnetic nanoadsorbents for micropollutant removal in real water treatment: a review.

Environ Chem Lett 2021 Jul 29:1-21. Epub 2021 Jul 29.

Environmental Engineering and Management Research Group, Ton Duc Thang University, Ho Chi Minh City, Vietnam.

Pure water will become a golden resource in the context of the rising pollution, climate change and the recycling economy, calling for advanced purification methods such as the use of nanostructured adsorbents. However, coming up with an ideal nanoadsorbent for micropollutant removal is a real challenge because nanoadsorbents, which demonstrate very good performances at laboratory scale, do not necessarily have suitable properties in in full-scale water purification and wastewater treatment systems. Here, magnetic nanoadsorbents appear promising because they can be easily separated from the slurry phase into a denser sludge phase by applying a magnetic field. Read More

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Manual separation of labial synechiae: A cost-effective method in prepubertal girls.

Afr J Paediatr Surg 2021 Jul-Sep;18(3):139-142

Department of Paediatric Surgery, Pakistan Institute of Medical Sciences, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad, Pakistan.

Background: Labial synechiae is a fairly common gynaecological problem that occurs as a result of inflammation leading to fusion between the labia minora. Being in a developing country with limited resources and poor compliance of patients to prolonged treatments, we are faced with immense challenges in the management of these girls.

Aims: We wanted to evaluate the efficacy and the cost-effectiveness of manual separation and topical antibiotics with perineal hygiene in the management of pre-pubertal girls with labial synechiae. Read More

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Purification of Labeled Oligonucleotides by Chromatography on a Sep-Pak C Column.

Cold Spring Harb Protoc 2021 Aug 2;2021(8):pdb.prot100727. Epub 2021 Aug 2.

This protocol describes a method for the separation of radiolabeled oligonucleotides from unincorporated radiolabel that takes advantage of the reversible affinity of oligonucleotides for silica gel. This protocol can be used only to purify oligonucleotides carrying a 5'-phosphate group, radiolabeled or unlabeled. Read More

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Analytical gradients for core-excited states in the algebraic diagrammatic construction (ADC) framework.

J Chem Phys 2021 Jul;155(4):044106

Department of Theoretical Chemistry and Biology, KTH Royal Institute of Technology, 10691 Stockholm, Sweden.

Expressions for analytical molecular gradients of core-excited states have been derived and implemented for the hierarchy of algebraic diagrammatic construction (ADC) methods up to extended second-order within the core-valence separation (CVS) approximation. We illustrate the use of CVS-ADC gradients by determining relaxed core-excited state potential energy surfaces and optimized geometries for water, formic acid, and benzene. For water, our results show that in the dissociative lowest core-excited state, a linear configuration is preferred. Read More

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Deep low-Rank plus sparse network for dynamic MR imaging.

Med Image Anal 2021 Jul 24;73:102190. Epub 2021 Jul 24.

Research Center for Medical AI, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China; Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China; Pazhou Lab, Guangzhou, China. Electronic address:

In dynamic magnetic resonance (MR) imaging, low-rank plus sparse (L+S) decomposition, or robust principal component analysis (PCA), has achieved stunning performance. However, the selection of the parameters of L+S is empirical, and the acceleration rate is limited, which are common failings of iterative compressed sensing MR imaging (CS-MRI) reconstruction methods. Many deep learning approaches have been proposed to address these issues, but few of them use a low-rank prior. Read More

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Controllable organosilane monolayer density of surface bonding using silatranes for thiol functionalization of silica particles for liquid chromatography and validation of microanalytical method for elemental composition determination.

J Chromatogr A 2021 Jul 22;1653:462418. Epub 2021 Jul 22.

Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analyses, University of Tübingen, Auf der Morgenstelle 8, Tübingen 72076, Germany. Electronic address:

The present work systematically investigates a new strategy for the functionalization of silica gel using alkyl silatrane chemistry instead of alkylsilanes for synthesis of chromatographic stationary phases. In this work, silica was chemically modified for further functionalization by a thiol-ene click reaction. Thus, 3-mercaptopropylsilatrane (MPS) was used which is capable to form self-assembled monolayers (SAM) on top of silanol surfaces in a controlled manner as previously shown for silicon wafers. Read More

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Stimulant Treatment Effect on Anxiety Domains in Children with Attention-Deficit/Hyperactivity Disorder With and Without Anxiety Disorders: A 12-Week Open-Label Prospective Study.

J Child Adolesc Psychopharmacol 2021 Jul 30. Epub 2021 Jul 30.

The Child Psychiatry Division, Sheba Medical Center, Edmond and Lily Safra Children's Hospital, Ramat Gan, Israel.

The effect of stimulants on anxiety domains has not been systematically studied. We assessed prospectively the impact of stimulant treatment in children with attention-deficit/hyperactivity disorder (ADHD) on the severity of anxiety domains and on ADHD with comorbid anxiety disorders. Children with ADHD ( = 57, aged 6-15 years) started a stimulant or were switched from one stimulant to another. Read More

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The horizontal shear fracture of the pelvis.

Eur J Trauma Emerg Surg 2021 Aug 2. Epub 2021 Aug 2.

Discipline of Surgery, School of Medicine and Public Health, University of Newcastle, Newcastle, NSW, Australia.

Purpose: Various classification systems describe fractures of the acetabulum and pelvis separately. Horizontal shear fractures involve the pelvic ring and both acetabula and have not been previously described. The aim of this study is to describe the horizontal shear fracture of the pelvis. Read More

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Development and validation of a RP-UPLC method for the determination of betamethasone dipropionate impurities in topical formulations using a multivariate central composite design.

Anal Methods 2021 Aug 2. Epub 2021 Aug 2.

Analytical Research and Development, Douglas Pharma US Inc, 1035 Louis Drive, Warminster, Pennsylvania, USA18974.

The current study presents a specific, accurate, simple, and rapid UPLC method for the determination of impurities present in cream and ointment formulations of betamethasone dipropionate (BMD). The analytical method was optimized using central composite design (CCD) prior to the method validation. Critical Process Parameters (CPPs) and Critical Quality Attributes (CQAs) were identified for the analytical method. Read More

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Clinical utility of Abbott Alinity hq extended red blood cell parameters in differentiating β-thalassemia trait and iron deficiency anemia.

Int J Lab Hematol 2021 Aug 1. Epub 2021 Aug 1.

Clinical Pathology Department, "San Giovanni Calibita" Fatebenefratelli Hospital, Rome, Italy.

Introduction: The objective of the study was to evaluate the performance of the Abbott Alinity hq advanced multi angle polarized scatter separation (MAPSS )-based optical RBC technology, for the differentiation between iron deficiency anemia (IDA) and β-thalassemia carrier status.

Methods: Four hundred and sixty-four samples were analyzed. 228 were healthy controls, 30 were β-thalassemia carriers, and 40 were IDA. Read More

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Simultaneous Determination of Hydrochlorothiazide and Losartan Potassium in Pharmaceutical Product by UV-Vis Spectrophotometric Method with Kalman Filter Algorithm.

J Anal Methods Chem 2021 20;2021:2754133. Epub 2021 Jul 20.

Faculty of Environmental Sciences, Saigon University, Ho Chi Minh City 700000, Vietnam.

In this paper, hydrochlorothiazide (HCT) and losartan potassium (LSP) in tablets are simultaneously determined with UV-Vis spectrophotometry and chemometrics without separation. The spectra of standard and sample solutions were recorded in the wavelength from 220 to 300 nm at 1.0 nm intervals. Read More

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Liquid-liquid phase separation in human health and diseases.

Signal Transduct Target Ther 2021 Aug 2;6(1):290. Epub 2021 Aug 2.

Institutes of Biology and Medical Science, Soochow University, Suzhou, People's Republic of China.

Emerging evidence suggests that liquid-liquid phase separation (LLPS) represents a vital and ubiquitous phenomenon underlying the formation of membraneless organelles in eukaryotic cells (also known as biomolecular condensates or droplets). Recent studies have revealed evidences that indicate that LLPS plays a vital role in human health and diseases. In this review, we describe our current understanding of LLPS and summarize its physiological functions. Read More

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