407,777 results match your criteria sensitive method

Increased lipocalin 2 levels in adolescents with type 2 diabetes mellitus.

J Pediatr Endocrinol Metab 2021 Jun 14. Epub 2021 Jun 14.

Department of Pediatrics, Kansai Medical University, Hirakata, Osaka, Japan.

Objectives: Bone can act as an endocrine organ through the secretion of bone-specific hormones, i.e., osteokines. Read More

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Quantitative detection of aflatoxin B using quantum dots-based immunoassay in a recyclable gravity-driven microfluidic chip.

Biosens Bioelectron 2021 Jun 2;190:113394. Epub 2021 Jun 2.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China. Electronic address:

To achieve rapid and sensitive detection of aflatoxin B (AFB), we developed a polydimethylsiloxane gravity-driven cyclic microfluidic chip using the two-signal mode strategy. The structural design of the chip, together with the two-wavelength quantum dot ratio fluorescence, effectively eliminates the influence of environmental factors, improves the signal stability, and ensures that the final detection result positively correlates with the target concentration. Moreover, the theoretical analysis performed for the established physical model of the three-dimensional reaction interface inside the chip confirmed the improved reaction rate of immune adsorption in the microfluidic strategy. Read More

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Feasibility and acceptability of mindful recovery opioid use care continuum (M-ROCC): A concurrent mixed methods study.

J Subst Abuse Treat 2021 Apr 15;130:108415. Epub 2021 Apr 15.

Harvard Medical School, Boston, MA, United States of America; Cambridge Health Alliance, Cambridge, MA, United States of America; Geisel School of Medicine at Dartmouth College, Hanover, NH, United States of America. Electronic address:

As opioid overdose deaths increase, buprenorphine/naloxone (B/N) treatment is expanding, yet almost half of patients are not retained in B/N treatment. Mindfulness-based interventions (MBIs) designed to promote non-judgmental awareness of present moment experience may be complementary to B/N treatment and offer the potential to enhance retention by reducing substance use and addressing comorbid symptoms. In this pilot study, we examined the feasibility and acceptability of the Mindful Recovery OUD Care Continuum (M-ROCC), a trauma-informed, motivationally sensitive, 24-week MBI. Read More

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K channel blockers control glucagon secretion by distinct mechanisms: a direct stimulation of α-cells involving a [Ca] rise and an indirect inhibition mediated by somatostatin.

Mol Metab 2021 Jun 9:101268. Epub 2021 Jun 9.

Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Pôle d'Endocrinologie, Diabète et Nutrition, Brussels, Belgium. Electronic address:

Objective: Glucagon is secreted by pancreatic α-cells in response to hypoglycemia and its hyperglycemic effect helps to restore normal blood glucose. Insulin and somatostatin (SST) secretions from β- and δ-cells, respectively, are stimulated by glucose by mechanisms implicating an inhibition of their ATP-sensitive K (K) channels, leading to an increase in [Ca] that triggers exocytosis. Drugs that close K channels, such as sulfonylureas, are used to stimulate insulin release in type 2 diabetic patients. Read More

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A comparison of SARS-CoV-2 nucleocapsid and spike antibody detection using three commercially available automated immunoassays.

Clin Biochem 2021 Jun 9. Epub 2021 Jun 9.

Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Health System, Lebanon, NH, United States; he Geisel School of Medicine at Dartmouth, Hanover, NH, United States.

Introduction: Commercially available serological assays for SARS-CoV-2 detect antibodies to either the nucleocapsid or spike protein. Here we compare the performance of the Beckman-Coulter SARS-CoV-2 spike IgG assay to that of the Abbott SARS-CoV-2 nucleocapsid IgG and Roche Anti-SARS-CoV-2 nucleocapsid total antibody assays. In addition, we document the trend in nucleocapsid and spike antibodies in sequential samples collected from convalescent plasma donors. Read More

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Autotransporter-based surface expression and complementation of split TreA fragments utilized for the detection of antibodies against bovine leukemia virus.

J Immunol Methods 2021 Jun 9:113084. Epub 2021 Jun 9.

Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada. Electronic address:

Bovine Leukemia Virus (BLV) is an oncogenic virus which is the etiological agent of a neoplastic disease in infected cattle called enzootic bovine leukemia (EBL). The most common and sensitive diagnostic methods for EBL like enzyme-linked immunosorbent assay (ELISA) is time-consuming and requires manual handling which makes it unsuitable as an on-farm diagnostic test. Hence, there is a need for an alternative test with rapid detection and reduced manual labour. Read More

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Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography -Tandem Mass Spectrometry.

Methods Mol Biol 2021 ;2275:329-339

Laboratory division HUSLAB, Helsinki University Central Hospital, Helsinki, Finland.

Coenzyme Q10 (CoQ) is an essential part of the mitochondrial respiratory chain . Here, we describe an accurate and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of mitochondrial CoQ in isolated mitochondria . In the assay, mitochondrial suspensions are spiked with CoQ-[H] internal standard (IS), extracted with organic solvents and CoQ quantified by LC-MS/MS using multiple reaction monitoring (MRM). Read More

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January 2021

The instep flap for anterior ankle coverage with bone and hardware exposure.

Eur J Orthop Surg Traumatol 2021 Jun 12. Epub 2021 Jun 12.

Division of Orthopaedic and Trauma Surgery, Sahel General Hospital, University Medical Centre, PO Box 99/25., Ghoubeiry, Beirut, Lebanon.

Purpose: The instep medial plantar flap is a well-known flap based on the medial plantar artery of the foot and usually used for coverage of soft tissue defects of the heel area. It has seldom been reported for coverage of anterior ankle area with exposure of the bone and metallic hardware after open reduction and internal fixation of distal tibial fractures. The primary purpose of this study is to evaluate the feasibility and viability of this flap as well as its reliability saving the internal fixation devices and efficiency protecting bone healing; the secondary purpose is to assess the condition of the flap and its cosmetic appearance, as well as occurrence of complications related to its harvesting. Read More

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Geonomics: forward-time, spatially explicit, and arbitrarily complex landscape genomic simulations.

Mol Biol Evol 2021 Jun 12. Epub 2021 Jun 12.

Department of Environmental Science, Policy, and Management, College of Natural Resources, University of California, Berkeley, CA, 94720, USA.

Understanding the drivers of spatial patterns of genomic diversity has emerged as a major goal of evolutionary genetics. The flexibility of forward-time simulation makes it especially valuable for these efforts, allowing for the simulation of arbitrarily complex scenarios in a way that mimics how real populations evolve. Here, we present Geonomics, a Python package for performing complex, spatially explicit, landscape genomic simulations with full spatial pedigrees that dramatically reduces user workload yet remains customizable and extensible because it is embedded within a popular, general-purpose language. Read More

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Toys or Tools? Utilization of Unmanned Aerial Systems in Mosquito and Vector Control Programs.

J Econ Entomol 2021 Jun 12. Epub 2021 Jun 12.

Hudson Regional Health Commission, Secaucus, NJ 07094, USA.

Organized mosquito control programs (MCP) in the United States have been protecting public health since the early 1900s. These programs utilize integrated mosquito management for surveillance and control measures to enhance quality of life and protect the public from mosquito-borne diseases. Because much of the equipment and insecticides are developed for agriculture, MCP are left to innovate and adapt what is available to accomplish their core missions. Read More

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Indocyanine green is a sensitive adjunct in the identification and surgical management of local and metastatic hepatoblastoma.

Cancer Med 2021 Jun 12. Epub 2021 Jun 12.

Division of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.

Background: Hepatoblastoma is the most common primary pediatric liver malignancy. Indocyanine green (ICG) has been described as an adjunct to resection in small series. Its utility remains undefined in larger cohorts. Read More

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Chronic restraint stress induces changes in the cerebral Galpha 12/13 and Rho-GTPase signaling network.

Pharmacol Rep 2021 Jun 11. Epub 2021 Jun 11.

Department of Brain Biochemistry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343, Kraków, Poland.

Background: Evidence indicates that Gα12, Gα13, and its downstream effectors, RhoA and Rac1, regulate neuronal morphology affected by stress. This study was aimed at investigating whether repeated stress influences the expression of proteins related to the Gα12/13 intracellular signaling pathway in selected brain regions sensitive to the effects of stress. Furthermore, the therapeutic impact of β(1)adrenergic receptors (β1AR) blockade was assessed. Read More

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Glutathione-Conjugated Hydrogels: Flexible Vehicles for Personalized Treatment of Bacterial Infections.

Pharm Res 2021 Jun 11. Epub 2021 Jun 11.

Department of Pharmaceutical Sciences, University of Illinois at Chicago, Chicago, Illinois, USA.

Purpose: Skin and soft tissue infections are increasingly prevalent and often complicated by potentially fatal therapeutic hurdles, such as poor drug perfusion and antibiotic resistance. Delivery vehicles capable of versatile loading may improve local bioavailability and minimize systemic toxicities yet such vehicles are not clinically available. Therefore, we aimed to expand upon the use of glutathione-conjugated poly(ethylene glycol) GSH-PEG hydrogels beyond protein delivery and evaluate the ability to deliver traditional therapeutic molecules. Read More

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Highly sensitive active pixel image sensor array driven by large-area bilayer MoS transistor circuitry.

Nat Commun 2021 Jun 11;12(1):3559. Epub 2021 Jun 11.

School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Republic of Korea.

Various large-area growth methods for two-dimensional transition metal dichalcogenides have been developed recently for future electronic and photonic applications. However, they have not yet been employed for synthesizing active pixel image sensors. Here, we report on an active pixel image sensor array with a bilayer MoS film prepared via a two-step large-area growth method. Read More

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Bed-Sharing in the First 6 Months: Associations with Infant-Mother Attachment, Infant Attention, Maternal Bonding, and Sensitivity at 18 Months.

J Dev Behav Pediatr 2021 May 20. Epub 2021 May 20.

School of Psychology, University of Kent, Canterbury, United Kingdom; Department of Psychology, University of Warwick, Coventry, United Kingdom; Division of Mental Health and Wellbeing, Warwick Medical School, University of Warwick, Coventry, United Kingdom.

Objective: The objective of this study was to investigate whether bed-sharing during the first 6 months of life is associated with infant's attachment and behavioral outcomes and mother's bonding and sensitive parenting at 18 months of age.

Methods: The sample with complete longitudinal data comprised 178 infants and their caretakers. Bed-sharing was assessed with maternal report at term, 3, 6, and 18 months. Read More

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Phase II clinical trial using anti-CD3 × anti-HER2 bispecific antibody armed activated T cells (HER2 BATs) consolidation therapy for HER2 negative (0-2+) metastatic breast cancer.

J Immunother Cancer 2021 Jun;9(6)

Medicine, University of Virginia, Charlottesville, Virginia, USA

Background: Metastatic human epidermal growth receptor II (HER2) negative breast cancer remains incurable. Our phase I study showed that anti-CD3 × anti-HER2 bispecific antibody armed activated T cells (HER2 BATs) may be effective against HER2-tumors. This phase II trial evaluates the efficacy and immune responses of HER2 BATs given to patients with metastatic HER2-estrogen and/or progesterone receptor positive (HR+) and triple negative breast cancer (TNBC) as immune consolidation after chemotherapy. Read More

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Use of Rapid Assessment Procedures when analyzing qualitative data in pharmacy research.

Res Social Adm Pharm 2021 Jun 6. Epub 2021 Jun 6.

Department of Clinical Pharmacy and Translational Science, University of Tennessee Health Science Center, College of Pharmacy, Nashville, TN, USA. Electronic address:

Qualitative research analytics and methodology are a useful part of many research projects. However, qualitative data analysis may be time intensive causing delays in results. This is especially problematic in time-sensitive projects where there an urgent need for results and a rapidly evolving situation being studied, such as during health crisis or early stages of project implementation. Read More

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Cost Effectiveness of an Electrocardiographic Deep Learning Algorithm to Detect Asymptomatic Left Ventricular Dysfunction.

Mayo Clin Proc 2021 Jun 8. Epub 2021 Jun 8.

Department of Cardiovascular Medicine, Mayo Clinic, Rochester, MN.

Objective: To evaluate the cost-effectiveness of an artificial intelligence electrocardiogram (AI-ECG) algorithm under various clinical and cost scenarios when used for universal screening at age 65.

Patients And Methods: We used decision analytic modeling to perform a cost-effectiveness analysis of the use of AI-ECG to screen for asymptomatic left ventricular dysfunction (ALVD) once at age 65 compared with no screening. This screening consisted of an initial screening decision tree and subsequent construction of a Markov model. Read More

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Legalization of cannabis use in Canada: Impacts on the cannabis use profiles of youth seeking services for substance use.

J Subst Abuse Treat 2021 Jul 25;126:108340. Epub 2021 Feb 25.

Margaret and Wallace McCain Centre for Child, Youth and Family Mental Health, Centre for Addiction and Mental Health, Toronto, Canada; Department of Psychiatry, University of Toronto, Toronto, Canada. Electronic address:

Background: Cannabis is a highly used substance among youth, but has the potential to negatively impact the developing brain. Recreational cannabis use was legalized in Canada in October 2018. This exploratory study examines cannabis use profiles of high-risk youth before and after the national legalization of recreational cannabis use. Read More

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Monitoring the mechanism of anti-cancer agents to inhibit colorectal cancer cell proliferation: Enzymatic biosensing of glucose combined with molecular docking.

Enzyme Microb Technol 2021 Aug 21;148:109804. Epub 2021 Apr 21.

Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.

Glucose, a major energy source in cellular metabolism, has a significant role in cell growth. The increase in glucose uptake is a distinguishing hallmark in cancer cells. A key step in glucose utilization is the transport of glucose to the cancer cells for supplying their additional energy. Read More

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Development of a rapid and sensitive immunochromatographic strip based on EuNPs-ES fluorescent probe for the detection of early Trichinella spiralis-specific IgG antibody in pigs.

Vet Res 2021 Jun 11;52(1):85. Epub 2021 Jun 11.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.

Trichinellosis, which is caused by nematodes of the genus Trichinella, is one of the most important zoonotic parasite diseases in the world. A rapid and sensitive immunochromatographic strip (ICS) based on Eu (III) nanoparticles (EuNPs) was developed for the detection of Trichinella spiralis (T. spiralis) infection in pigs. Read More

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Is therapeutic inertia present in hyperglycaemia, hypertension and hypercholesterolaemia management among adults with type 2 diabetes in three health clinics in Malaysia? a retrospective cohort study.

BMC Fam Pract 2021 Jun 11;22(1):111. Epub 2021 Jun 11.

Seri Kembangan Health Clinic, Taman Muhibbah, Jalan Besar43300 Seri Kembangan, Selangor, Malaysia.

Background: Good-quality evidence has shown that early glycaemic, blood pressure and LDL-cholesterol control in people with type 2 diabetes (T2D) leads to better outcomes. In spite of that, diseases control have been inadequate globally, and therapeutic inertia could be one of the main cause. Evidence on therapeutic inertia has been lacking at primary care setting. Read More

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Instrumentational implementation for parallelized nanopore electrochemical measurements.

Analyst 2021 Jun 12. Epub 2021 Jun 12.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 210023, Nanjing, China.

Nanopore electrochemistry, as one of the promising tools for single molecule sensing, has proved its capability in DNA sequencing and protein analysis. To achieve a high resolution for obtaining molecular information, the nanopore electrochemical technique not only urgently requires an appropriate nanopore sensing interface with atomic resolution but also requires advanced instrumentation and its related data processing methods. In order to reveal the fundamental biological process and process the point-of-care diagnosis, it is necessary to use a nanopore sensing instrument with a high amperometric and temporal resolution as well as high throughput. Read More

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Evaluation of IGF-1 as a novel theranostic biomarker for schizophrenia.

J Psychiatr Res 2021 Jun 2;140:172-179. Epub 2021 Jun 2.

Department of Psychiatry, Bakirkoy Prof Mazhar Osman Training and Research Hospital for Psychiatry, Neurology, and Neurosurgery, Istanbul, Turkey.

Objective: In the current study, we aimed to investigate fasting plasma levels of glucose, insulin, growth hormone, IGF-1, and lipid profile in remission schizophrenia patients, treatment resistant schizophrenia patients and healthy controls and to determine whether IGF-1 levels can be used as a theranostic biomarker in schizophrenia.

Methods: Sixty-two patients under remission from schizophrenia, sixty-five treatment-resistant patients with schizophrenia and sixty-two healthy controls were included in the study. All patients were recruited and evaluated over 11 months. Read More

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Adjuvant endocrine therapy for premenopausal women with breast cancer: Patient adherence and physician prescribing practices in Mexico.

Breast 2021 Jun 1;59:8-15. Epub 2021 Jun 1.

Departamento de Tumores Mamarios e Investigacion, Instituto Nacional de Cancerologia, San Fernando 22, Belisario Dominguez Secc 16, Tlalpan, Mexico City, 14080, Mexico.

Background: In resource-constrained settings, data regarding breast cancer patients' adherence to endocrine therapy (ET) and physicians' prescribing practices is limited. This study aims to decrease this knowledge gap in a real-world clinical practice.

Methods: Premenopausal women with stage 0-III hormone-sensitive breast cancer and receiving adjuvant ET during the past 1-5 years were identified in three Mexican referral centers. Read More

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Rapid determination of viable but non-culturable Campylobacter jejuni in food products by loop-mediated isothermal amplification coupling propidium monoazide treatment.

Int J Food Microbiol 2021 Jun 1;351:109263. Epub 2021 Jun 1.

Food Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada; Department of Food Science and Agricultural Chemistry, Faculty of Agricultural and Environmental Sciences, McGill University, Ste Anne de Bellevue, QC H9X 3V9, Canada. Electronic address:

Campylobacter is the leading cause of foodborne human diarrhea worldwide. This microbe in the viable but non-culturable (VBNC) state can evade detection by routinely used culture-based methods and remain viable for extended periods of time. Bacteria in this dormancy state can resume their metabolic activity and virulence by resuscitation under favorable conditions, and subsequently cause infections. Read More

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Gefitinib with concurrent thoracic radiotherapy in unresectable locally advanced non-small cell lung cancer with EGFR mutation; West Japan Oncology Group 6911L.

J Thorac Oncol 2021 Jun 8. Epub 2021 Jun 8.

Department of Medical Oncology, Kindai University Faculty of Medicine, 377-2 Ohno-higashi, Osakasayama, Japan.

Introduction: About 10% of locally-advanced non-small cell lung cancer (LA-NSCLC) patients harbor epidermal growth factor receptor (EGFR) mutation and recent reports suggested the declined benefit with immune-checkpoint inhibitor in this population. The attempt that introduces EGFR-tyrosine kinase inhibitor (TKI) into the treatment of LA-NSCLC with EGFR mutation has been warranted.

Methods: Chemotherapy-naïve, unresectable LA-NSCLC patients with sensitive EGFR mutation (exon 19 deletion or exon 21 L858R point mutation) were enrolled. Read More

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Exercise attenuates low back pain and alters epigenetic regulation in intervertebral discs in a mouse model.

Spine J 2021 Jun 8. Epub 2021 Jun 8.

Faculty of Dentistry, McGill University, Montreal, Quebec, H3A 0G1, Canada; Alan Edwards Centre for Research on Pain, McGill University, Montreal, Quebec, H3A 0G1, Canada; Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba City, Chiba, Japan, 260-8677; Faculty of Medicine, Departments of Pharmacology and Toxicology, Anesthesiology and Neurology and Neurosurgery, McGill University, Montreal, Quebec, H3A 0G1, Canada; Faculty of Medicine, Department of Anesthesiology, University of Minnesota, Minneapolis, MN, 55455, USA. Electronic address:

Background Context: Chronic low back pain (LBP) is a multifactorial disorder with complex underlying mechanisms, including associations with intervertebral disc (IVD) degeneration in some individuals. It has been demonstrated that epigenetic processes are involved in the pathology of IVD degeneration. Epigenetics refers to several mechanisms, including DNA methylation, that have the ability to change gene expression without inducing any change in the underlying DNA sequence. Read More

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One-Pot Synthesis of Carboxymethyl-Dextran Coated Iron Oxide Nanoparticles (CION) for Preclinical fMRI and MRA Applications.

Neuroimage 2021 Jun 8:118213. Epub 2021 Jun 8.

Center for Animal MRI, University of North Carolina, Chapel Hill, NC, USA; Biomedical Research Imaging Center, University of North Carolina, Chapel Hill, NC, USA; Department of Neurology, University of North Carolina, Chapel Hill, NC, USA. Electronic address:

Superparamagnetic iron-oxide nanoparticles are robust contrast agents for magnetic resonance imaging (MRI) and has shown promise for sensitive structural and functional mapping of the cerebral blood volume (CBV) when administered intravenously. To date, many CBV-MRI studies are conducted with Feraheme, manufactured for the treatment of iron-deficiency. Unfortunately, Feraheme is currently not available outside the United States due to commercial and regulatory constraints, making the CBV-MRI method either inaccessible or very costly to achieve. Read More

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Novel universal primer-pentaplex PCR assay based on chimeric primers for simultaneous detection of five common pig viruses associated with diarrhea.

Mol Cell Probes 2021 Jun 8:101747. Epub 2021 Jun 8.

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China. Electronic address:

Viral pathogens associated with diarrhea in pigs include porcine circovirus 2 (PCV2), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus A (RVA) and C (RVC) among others. In this study, a novel universal primer-based pentaplex PCR (UP-M-PCR) assay was developed for simultaneous detection and differentiation of these five viruses. The assay uses a short-cycle multiplex amplification by chimeric primers (CP), which are virus specific, with a tail added at the 5' end of the universal primer (UP), followed by universal amplification using UPs and a regular cycle amplification. Read More

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