1,000 results match your criteria seeding behavior

Of 'junk food' and 'brain food': how parental diet influences offspring neurobiology and behaviour.

Trends Endocrinol Metab 2021 Apr 30. Epub 2021 Apr 30.

Department of Medical Sciences, Adelaide Medical School, The University of Adelaide, Adelaide, SA 5005, Australia. Electronic address:

Unhealthy lifestyles and mental health problems are increasingly prevalent globally. Not only are 'junk food'-induced overweight and obesity risk factors for the development of brain disorders but they are also associated intergenerationally with ill health. Here, we reflect on the current knowledge of how maternal and paternal diet influences offspring brain development and behaviour, potentially predisposing children to mental health problems. Read More

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Fleeing lockdown and its impact on the size of epidemic outbreaks in the source and target regions - a COVID-19 lesson.

Sci Rep 2021 04 29;11(1):9233. Epub 2021 Apr 29.

Bolyai Institute, University of Szeged, Szeged, 6720, Hungary.

The COVID-19 pandemic forced authorities worldwide to implement moderate to severe restrictions in order to slow down or suppress the spread of the disease. It has been observed in several countries that a significant number of people fled a city or a region just before strict lockdown measures were implemented. This behavior carries the risk of seeding a large number of infections all at once in regions with otherwise small number of cases. Read More

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Inter-laboratory variability of A549 epithelial cells grown under submerged and air-liquid interface conditions.

Toxicol In Vitro 2021 Apr 24:105178. Epub 2021 Apr 24.

BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland. Electronic address:

In vitro cell models offer a unique opportunity for conducting toxicology research, and human lung adenocarcinoma cell line A549 is commonly used for toxicology testing strategies. It is essential to determine whether the response of these cells grown in different laboratories is consistent. In this study, A549 cells were grown under both submerged and air-liquid interface (ALI) conditions following an identical cell seeding protocol in two independent laboratories. Read More

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Synthesis of FAU-Zeolite Membrane by a Secondary Growth Method: Influence of Seeding on Membrane Growth and Its Performance in the Dehydration of Isopropyl Alcohol-Water Mixture.

ACS Omega 2021 Apr 31;6(14):9834-9842. Epub 2021 Mar 31.

Department of Applied Chemistry, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.

Y-type zeolite membranes were prepared on a porous tubular α-alumina support by a secondary growth process. Various experimental conditions such as seed size, pH of seed solution, and degassing of support were examined for understanding their influence on the membrane deposition process. The experimental results showed that the potential of alumina support surface and the USY seed slurry plays a significant role in controlling the electrostatic interaction between seed particles and support surface and also the aggregation of USY seed particles in the slurry. Read More

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Alpha-synuclein seeds in olfactory mucosa of patients with isolated REM sleep behaviour disorder.

Brain 2021 Apr 14. Epub 2021 Apr 14.

Department of Neurosciences, Biomedicine and Movement Sciences University of Verona, Verona, Italy.

Isolated REM sleep behaviour disorder (RBD) is an early-stage α-synucleinopathy in most, if not all, affected subjects. Detection of pathological α-synuclein in peripheral tissues of patients with isolated RBD may identify those progressing to Parkinson's disease, dementia with Lewy bodies or multiple system atrophy, with the ultimate goal of testing preventive therapies. Real-time quaking-induced conversion (RT-QuIC) provided evidence of α-synuclein seeding activity in CSF and olfactory mucosa of patients with α-synucleinopathies. Read More

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Measuring temporal patterns in ecology: The case of mast seeding.

Ecol Evol 2021 Apr 11;11(7):2990-2996. Epub 2021 Mar 11.

CSIC Global Ecology Unit CREAF-CSIC-UAB Bellaterra Barcelona Spain.

Properly assessing temporal patterns is a central issue in ecology in order to understand ecosystem processes and their mechanisms. Mast seeding has traditionally been described as a reproductive behavior consisting of highly variable and synchronized reproductive events. The most common metric used to measure temporal variability and thus infer masting behavior, the coefficient of variation (CV), however, has been repeatedly suggested to improperly estimate temporal variability. Read More

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Tryptophan Probes of TDP-43 C-Terminal Domain Amyloid Formation.

J Phys Chem B 2021 Apr 9;125(15):3781-3789. Epub 2021 Apr 9.

Laboratory of Protein Conformation and Dynamics, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, United States.

Aggregated TAR DNA-binding protein 43 (TDP-43) forms the cytoplasmic hallmarks associated with patients suffering from amyotrophic lateral sclerosis and frontotemporal lobar degeneration with ubiquitin. Under normal conditions, TDP-43 is a 414-amino acid protein; however, aggregates are enriched with N-terminal truncations which contain residues 267-414, known as the C-terminal domain of TDP-43 (TDP-43). To gain residue-specific information on the aggregation process of TDP-43, we created three single-Trp containing mutants (W385F/W412F, W334F/W412F, and W334F/W385F) by substituting two of the three native Trp residues with Phe, yielding fluorescent probes at W334, W385, and W412, respectively. Read More

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Can psychological flexibility and prosociality mitigate illness perceptions toward COVID-19 on mental health? A cross-sectional study among Hong Kong adults.

Global Health 2021 04 8;17(1):43. Epub 2021 Apr 8.

Department of Psychology, University of Cyprus, Nicosia, Cyprus.

Background: The negative impact of COVID-19 pandemic on public mental health can be persistent and substantial over a long period of time, but little is known regarding what psychological factors or processes can buffer such impact. The present study aimed to examine the mediating roles of coping, psychological flexibility and prosociality in the impacts of perceived illness threats toward COVID-19 on mental health.

Method: Five-hundred and fourteen Hong Kong citizens (18 years or above) completed an online survey to measure illness perceptions toward COVID-19, coping, psychological flexibility, prosociality, and mental health, together with their socio-demographic variables. Read More

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Interactions between magnetic particles and polyaluminum chloride on the coagulation behavior in humic acid-kaolin synthetic water treatment.

Environ Res 2021 Apr 1;197:111093. Epub 2021 Apr 1.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China. Electronic address:

Understanding the interactions between magnetic particles (MPs) and polyaluminum chloride (PACl) is essential to elucidate the magnetic seeding coagulation (MSC) process. However, little is known about how MPs interact with the different Al species coexisting in the PACl. Here, the relationships among pollutants removal, residual Al distribution, and floc properties were comparatively studied in the MSC and traditional coagulation (TC) processes to address this issue. Read More

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Laryngeal carcinoma experimental model suggests the possibility of tumor seeding to gastrostomy site.

Med Hypotheses 2021 May 23;150:110573. Epub 2021 Mar 23.

Department of Histology and Embryology, Lithuanian University of Health Sciences, A. Mickevičiaus g. 9, Kaunas, LT 44307, Lithuania. Electronic address:

Some studies state that laryngeal squamous cell carcinoma (LSCC) is associated with possible direct tumor cell seeding to percutaneous endoscopic gastrostomy (PEG) site. However, there is a lack of experimental proof that LSCC tumor tissue can adhere and grow in distant sites. Therefore, we aimed to investigate the growth pattern of LSCC implants on chicken embryo chorioallantoic membrane (CAM) and evaluate possible associations between clinical course of the disease and behavior of experimentally implanted LSCC tumors. Read More

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The AD tau core spontaneously self-assembles and recruits full-length tau to filaments.

Cell Rep 2021 Mar;34(11):108843

Department of Neuroscience, Mayo Clinic, Jacksonville, FL, USA; Neuroscience Graduate Program, Mayo Clinic Graduate School of Biomedical Sciences, Jacksonville, FL, USA. Electronic address:

Tau accumulation is a major pathological hallmark of Alzheimer's disease (AD) and other tauopathies, but the mechanism(s) of tau aggregation remains unclear. Taking advantage of the identification of tau filament cores by cryoelectron microscopy, we demonstrate that the AD tau core possesses the intrinsic ability to spontaneously aggregate in the absence of an inducer, with antibodies generated against AD tau core filaments detecting AD tau pathology. The AD tau core also drives aggregation of full-length wild-type tau, increases seeding potential, and templates abnormal forms of tau present in brain homogenates and antemortem cerebrospinal fluid (CSF) from patients with AD in an ultrasensitive real-time quaking-induced conversion (QuIC) assay. Read More

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Tau assemblies do not behave like independently acting prion-like particles in mouse neural tissue.

Acta Neuropathol Commun 2021 03 12;9(1):41. Epub 2021 Mar 12.

Department of Clinical Neurosciences, UK Dementia Research Institute at the University of Cambridge, Cambridge, UK.

A fundamental property of infectious agents is their particulate nature: infectivity arises from independently-acting particles rather than as a result of collective action. Assemblies of the protein tau can exhibit seeding behaviour, potentially underlying the apparent spread of tau aggregation in many neurodegenerative diseases. Here we ask whether tau assemblies share with classical pathogens the characteristic of particulate behaviour. Read More

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A Novel SOD1 Intermediate Oligomer, Role of Free Thiols and Disulfide Exchange.

Front Neurosci 2020 18;14:619279. Epub 2021 Feb 18.

Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, United States.

Wild-type human SOD1 forms a highly conserved intra-molecular disulfide bond between C57-C146, and in its native state is greatly stabilized by binding one copper and one zinc atom per monomer rendering the protein dimeric. Loss of copper extinguishes dismutase activity and destabilizes the protein, increasing accessibility of the disulfide with monomerization accompanying disulfide reduction. A further pair of free thiols exist at C6 and C111 distant from metal binding sites, raising the question of their function. Read More

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February 2021

Study of bacterial interactions in reconstituted hydrocarbon-degrading bacterial consortia from a local collection, for the bioremediation of weathered oily-soils.

Biotechnol Rep (Amst) 2021 Mar 10;29:e00598. Epub 2021 Feb 10.

Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University. PO. Box 2713, Doha, Qatar.

To enhance the process of bacterial remediation of weathered hydrocarbons, the area of Dukhan, Qatar, was considered as a model for weathering processes. Self-purification by indigenous hydrocarbon-degrading bacteria showed low performance. Biostimulation/seeding using one or another of the indigenous bacteria improved the performance. Read More

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A novel biocompatible polymeric blend for applications requiring high toughness and tailored degradation rate.

J Mater Chem B 2021 03 4;9(10):2532-2546. Epub 2021 Mar 4.

Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands and the UWA Centre for Medical Research, The University of Western Australia, Perth, Australia.

Finding the right balance in mechanical properties and degradation rate of biodegradable materials for biomedical applications is challenging, not only at the time of implantation but also during biodegradation. For instance, high elongation at break and toughness with a mid-term degradation rate are required for tendon scaffold or suture application, which cannot be found in each alpha polyester individually. Here, we hypothesise that blending semi-crystalline poly(p-dioxanone) (PDO) and poly(lactide-co-caprolactone) (LCL) in a specific composition will enhance the toughness while also enabling tailored degradation times. Read More

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Suppression of α-synuclein propagation after intrastriatal injection in FABP3 null mice.

Brain Res 2021 Jun 24;1760:147383. Epub 2021 Feb 24.

Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan. Electronic address:

Accumulation and aggregation of α-synuclein (αSyn) trigger neuronal loss in the substantia nigra pars compacta (SNpc), which in turn causes motor symptoms in Parkinson's disease. We previously demonstrated that fatty acid-binding protein 3 (FABP3), an intracellular fatty acid carrier protein, enhances αSyn neurotoxicity in SNpc and motor impairments after intranigral injection of αSyn fibrils. However, the temporal profile of αSyn fibril spread and their toxicity remains unclear. Read More

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Genomic evolution and diverse models of systemic metastases in colorectal cancer.

Gut 2021 Feb 25. Epub 2021 Feb 25.

Department of Gastrointestinal Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Objective: The systemic spread of colorectal cancer (CRC) is dominated by the portal system and exhibits diverse patterns of metastasis without systematical genomic investigation. Here, we evaluated the genomic evolution of CRC with multiorgan metastases using multiregion sequencing.

Design: Whole-exome sequencing was performed on multiple regions (n=74) of matched primary tumour, adjacent non-cancerous mucosa, liver metastasis and lung metastasis from six patients with CRC. Read More

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February 2021

Flexible infrared photodetector based on indium antimonide nanowire arrays.

Nanotechnology 2021 Apr 14;32(27). Epub 2021 Apr 14.

Department of Physics, College of Sciences, United Arab Emirates University, Al Ain, 15551, United Arab Emirates.

Narrow bandgap semiconductors like indium antimonide (InSb) are very suitable for high-performance room temperature infrared photodetectors, but the fragile nature of the wafer materials hinders their application as flexible/wearable devices. Here, we present a method to fabricate a photodetector device of assembled crystalline InSb nanowire (NW) arrays on a flexible substrate that balances high performance and flexibility, facilitating its application in wearable devices. The InSb NWs were synthesized by means of a vapor-liquid-solid technique, with gold nanoclusters as seeding particles. Read More

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Amyloid cross-sequence interaction between Aβ(1-40) and αA(66-80) in relation to the pathogenesis of cataract.

Int J Biol Macromol 2021 May 21;179:61-70. Epub 2021 Feb 21.

Ulm University, Institute of Protein Biochemistry, Helmholtzstraße 8/1, 89081 Ulm, Germany; Department of Biotechnology, Central University of Rajasthan, NH-8 Bandarsindri, Kishangarh, Ajmer 305817, Rajasthan, India. Electronic address:

Alzheimer's disease (AD) and cataract represent two common protein misfolding diseases closely associated with aging. Growing evidence suggests that these two diseases may be interrelated with each other through cross-sequence interactions between β-amyloid (Aβ) peptide and the short aggregating peptides derived from proteolytic breakdown of α-crystallin. αΑ(66-80) is one of several peptides produced by the proteolytic breakdown of α-crystallin in aged eye lens. Read More

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Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.

J Clin Invest 2021 Feb;131(4)

Department of Pharmaceutical Sciences, University of South Florida, Tampa, Florida, USA.

Tauopathies display a spectrum of phenotypes from cognitive to affective behavioral impairments; however, mechanisms promoting tau pathology and how tau elicits behavioral impairment remain unclear. We report a unique interaction between polyamine metabolism, behavioral impairment, and tau fate. Polyamines are ubiquitous aliphatic molecules that support neuronal function, axonal integrity, and cognitive processing. Read More

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February 2021

Metastatic Dissemination Mimicked in a Multicellular Transwell Assay.

Methods Mol Biol 2021 ;2235:181-190

Vascular Oncology and Metastasis, German Cancer Research Center Heidelberg (DKFZ-ZMBH Alliance), Heidelberg, Germany.

Tumor progression and metastasis are multistep processes that are critically dependent on the interaction of metastasizing tumor cells with other cells of the local microenvironment. Mimicking the single steps of the metastatic cascade in vitro is therefore challenging when investigating not only tumor cell behavior alone but also cellular crosstalk between different cell populations. In particular, the crosstalk of tumor cells with pericytes and endothelial cells when accessing the bloodstream is of great importance for successful intravasation and metastatic dissemination. Read More

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A Network Thermodynamic Analysis of Amyloid Aggregation along Competing Pathways.

Appl Math Comput 2021 Mar 18;393. Epub 2020 Nov 18.

Department of Mathematics, Montclair State University, Montclair, NJ 07043.

Aggregation of proteins towards amyloid formation is a significant event in many neurodegenerative diseases. Low-molecular weight oligomers are considered to be the primary toxic agents in many of these maladies. Therefore, there is an increasing interest in understanding their formation and behavior. Read More

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Fully absorbable poly-4-hydroxybutyrate implants exhibit more favorable cell-matrix interactions than polypropylene.

Mater Sci Eng C Mater Biol Appl 2021 Jan 11;120:111702. Epub 2020 Nov 11.

Department of Obstetrics and Gynecology, Amsterdam Reproduction and Development, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, Amsterdam, the Netherlands. Electronic address:

Pelvic organ prolapse (POP) is a multifactorial condition characterized by the descent of the pelvic organs due to the loss of supportive tissue strength. This is presumably caused by the decreased fibroblast function and the subsequent change in the quality of the extracellular matrix. The correction of POP using an implant intends to provide mechanical support to the pelvic organs and to stimulate a moderate host response. Read More

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January 2021

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels.

J Vis Exp 2021 01 14(167). Epub 2021 Jan 14.

Faculty of Biomedical Engineering, Technion.

The cardiovascular system is a key player in human physiology, providing nourishment to most tissues in the body; vessels are present in different sizes, structures, phenotypes, and performance depending on each specific perfused tissue. The field of tissue engineering, which aims to repair or replace damaged or missing body tissues, relies on controlled angiogenesis to create a proper vascularization within the engineered tissues. Without a vascular system, thick engineered constructs cannot be sufficiently nourished, which may result in cell death, poor engraftment, and ultimately failure. Read More

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January 2021

Maternal influences on oral and faecal microbiota maturation in neonatal calves in beef and dairy production systems.

Anim Microbiome 2020 Sep 7;2(1):31. Epub 2020 Sep 7.

Department of Livestock and One Health, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool, Liverpool, UK.

Background: The dam is considered an important source of microbes for the calf; consequently, the development of calf microbiota may vary with farming system due to differences between the contact the calf has with the dam. The objective of this study was to characterise the early changes in the composition of oral and faecal microbiota in beef and dairy calves (N = 10) using high-throughput sequencing of the 16S rRNA gene. The microbiota of calves was compared to selected anatomical niches on their dams which were likely to contribute to the vertical transfer of microbes. Read More

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September 2020

Continuous Flow Chiral Amine Racemization Applied to Continuously Recirculating Dynamic Diastereomeric Crystallizations.

J Org Chem 2021 Feb 22;86(3):2458-2473. Epub 2021 Jan 22.

School of Chemistry and School of Chemical and Process Engineering. Institute of Process Research and Development. University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, U.K.

A new, dynamic diastereomeric crystallization method has been developed, in which the mother liquors are continuously separated, racemized over a fixed-bed catalyst, and recirculated to the crystallizer in a resolution-racemization-recycle (R) process. Separating the racemization from crystallization overcomes problems of using catalysts , that suffer conflicting sets of conditions, inhibition, and separation. Continuous racemization has been achieved through the covalent attachment of [IrCp*I] SCRAM catalyst to Wang resin solid support to give a fixed-bed catalyst. Read More

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February 2021

Anomalous Behavior in the Nucleation of Ice at Negative Pressures.

Phys Rev Lett 2021 Jan;126(1):015704

Departamento de Quimica Fisica, Facultad de Quimica, Universidad Complutense de Madrid, Ciudad Universitaria, Madrid 28040, Spain.

Ice nucleation is a phenomenon that, despite the relevant implications for life, atmospheric sciences, and technological applications, is far from being completely understood, especially under extreme thermodynamic conditions. In this work we present a computational investigation of the homogeneous ice nucleation at negative pressures. By means of the seeding technique we estimate the size of the ice critical nucleus N_{c} for the TIP4P/Ice water model. Read More

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January 2021

Peripherally misfolded proteins exacerbate ischemic stroke-induced neuroinflammation and brain injury.

J Neuroinflammation 2021 Jan 20;18(1):29. Epub 2021 Jan 20.

Division of Basic Biomedical Sciences and Center for Brain and Behavior Research, Sanford School of Medicine, University of South Dakota, Vermillion, SD, 57069, USA.

Background: Protein aggregates can be found in peripheral organs, such as the heart, kidney, and pancreas, but little is known about the impact of peripherally misfolded proteins on neuroinflammation and brain functional recovery following ischemic stroke.

Methods: Here, we studied the ischemia/reperfusion (I/R) induced brain injury in mice with cardiomyocyte-restricted overexpression of a missense (R120G) mutant small heat shock protein, αB-crystallin (CryAB), by examining neuroinflammation and brain functional recovery following I/R in comparison to their non-transgenic (Ntg) littermates. To understand how peripherally misfolded proteins influence brain functionality, exosomes were isolated from CryAB and Ntg mouse blood and were used to treat wild-type (WT) mice and primary cortical neuron-glia mix cultures. Read More

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January 2021

Three-Dimensional Printing of Hydrogel Scaffolds with Hierarchical Structure for Scalable Stem Cell Culture.

ACS Biomater Sci Eng 2020 05 29;6(5):2995-3004. Epub 2020 Apr 29.

Key Laboratory for Advanced Materials Processing Technology of Ministry of Education, Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.

The expansion and harvest of stem cells at clinically relevant scales is critical for cell-based therapies. These approaches need to be robust and cost-effective, support the functional maintenance of desired cell behaviors, and allow for simple harvest. Here, we introduce a real-time monitoring 3D printing approach to fabricate scaffolds with quadruple hierarchical structure that meet these design goals for stem cell expansion. Read More

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Integrating Microstructured Electrospun Scaffolds in an Open Microfluidic System for Studies of Human Patient-Derived Primary Cells.

ACS Biomater Sci Eng 2020 06 4;6(6):3649-3663. Epub 2020 May 4.

Institute of Biomedical Technologies, National Research Council of Italy, via F.lli Cervi 93, 20090 Segrate, Milan, Italy.

Recent studies have suggested that microenvironmental stimuli play a significant role in regulating cellular proliferation and migration, as well as in modulating self-renewal and differentiation processes of mammary cells with stem cell (SCs) properties. Recent advances in micro/nanotechnology and biomaterial synthesis/engineering currently enable the fabrication of innovative tissue culture platforms suitable for maintenance and differentiation of SCs Here, we report the design and fabrication of an open microfluidic device (OMD) integrating removable poly(ε-caprolactone) (PCL) based electrospun scaffolds, and we demonstrate that the OMD allows investigation of the behavior of human cells during culture in real time. Electrospun scaffolds with modified surface topography and chemistry can influence attachment, proliferation, and differentiation of mammary SCs and epigenetic mechanisms that maintain luminal cell identity as a function of specific morphological or biochemical cues imparted by tailor-made fiber post-treatments. Read More

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