J Plast Reconstr Aesthet Surg 2021 Apr 11. Epub 2021 Apr 11.
3F66, Block 3, Alderley Park, Nether Alderley, Cheshire, SK10 4TG; The Ex-Vivo Lab, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, United Kingdom; The Transplant Centre, Manchester University NHS Foundation Trust, Manchester M23 9LT, United Kingdom. Electronic address:
Introduction: The donor immune compartment plays a central role in graft rejection of the vascularised composite allograft (VCA) by contributing to 'direct presentation'. Using our limb ex vivo normothermic machine perfusion (EVNP) protocol designed for prolonged allograft preservation, this study aimed to assess whether donor leukocytes responsible for allograft rejection are mobilised from the donor compartment.
Methods: Five genetically different pig forelimbs underwent perfusion via the brachial and radial collateral artery for 6 h after 2 h of cold storage. Read More