Eukaryot Cell 2015 Jul 22;14(7):684-97. Epub 2015 May 22.
Section of Infectious Diseases, New Mexico VA Healthcare System, Albuquerque, New Mexico, USA Division of Infectious Diseases, University of New Mexico Health Science Center, Albuquerque, New Mexico, USA
The yeast exocyst is a multiprotein complex comprised of eight subunits (Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84) which orchestrates trafficking of exocytic vesicles to specific docking sites on the plasma membrane during polarized secretion. To study SEC6 function in Candida albicans, we generated a conditional mutant strain in which SEC6 was placed under the control of a tetracycline-regulated promoter. In the repressed state, the tetR-SEC6 mutant strain (denoted tSEC6) was viable for up to 27 h; thus, all phenotypic analyses were performed at 24 h or earlier. Read More