64,832 results match your criteria screening applications


Basic concepts of radiation emergency medicine.

J Radiol Prot 2021 Sep 15. Epub 2021 Sep 15.

Health Division, IRSN, Fontenay-aux-Roses, 92260, FRANCE.

Nuclear and radiological accidents are not frequent but may lead to major consequences in the population. For the health systems, the need to handle a large number of victims will probably remain as an exception. However, a high number of affected victims can be expected in some terrorist scenarios. Read More

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September 2021

Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.

Cell Rep 2021 Sep;36(11):109700

Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA; Integrative Molecular Biomedical Sciences Graduate Program, Baylor College of Medicine, Houston, TX 77030, USA; Dan L. Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, TX 77030, USA; McNair Medical Institute at The Robert and Janice McNair Foundation, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address:

The power of Drosophila melanogaster as a model system relies on tractable germline genetic manipulations. Despite Drosophila's expansive genetics toolbox, such manipulations are still accomplished one change at a time and depend predominantly on phenotypic screening. We describe a drug-based genetic platform consisting of four selection and two counterselection markers, eliminating the need to screen for modified progeny. Read More

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September 2021

Acousto-Pi: An Opto-Acoustofluidic System using Surface Acoustic Waves controlled with Open Source Electronics for Integrated In-Field Diagnostics.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Sep 15;PP. Epub 2021 Sep 15.

Surface acoustic wave (SAW) devices are increasingly applied in life science, biology, and point-of-care applications due to their combined acoustofluidic sensing and actuating properties. Despite the advances in this field, there remain significant gaps in interfacing hardware and control strategies to facilitate system integration with high performance and low cost. In this work, we present a versatile, and digitally controlled acoustofluidic platform by demonstrating key functions for biological assays such as droplet transportation and mixing using a closed-loop feedback control with image recognition. Read More

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September 2021

Multilevel Theoretical Screening of Novel Two-Dimensional MAZ Family for Hydrogen Evolution.

J Phys Chem Lett 2021 Sep 15:9149-9154. Epub 2021 Sep 15.

Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou, Jiangsu 215123, China.

The synthesis of 2D MoSiN marked the birth of a new family of 2D MAZ materials, whose potential applications are expected to sweep the field of nanoscale devices and catalysis in the future. In this work, we propose a multilevel screening workflow to systematically explore the mechanic stabilities, electronic properties, and hydrogen evolution performances of the 2D MAZ family, among which seven stable, metallic, and highly active 2D MAZ monolayers (2H-α-VGeN, 2H-α-NbGeN, 2H-α-TaGeN, 2H-α-NbSiN, 2H-β-VGeN, 2H-β-NbGeN, and 2H-β-TiGeP) are predicted as promising hydrogen evolution reaction (HER) catalysts with near-zero hydrogen adsorption free energy (Δ). The lowest unoccupied state energy () of the MAZ basal plane is identified as a concise descriptor to influence the electron filling and eventually determine its Δ. Read More

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September 2021

A review on production of polyhydroxyalkanoate (PHA) biopolyesters by thermophilic microbes using waste feedstocks.

Bioresour Technol 2021 Sep 8;341:125900. Epub 2021 Sep 8.

INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.

Polyhydroxyalkanoates (PHAs) are produced by numerous microbes as a subcellular energy source. Despite of their diverse applications, exorbitant production cost limits their commercial synthesis. Apart from various cost determining factors such as cost-effective feedstocks or economic recovery methods, the use of appropriate bacteria holds the key to reduce the fermentation economics. Read More

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September 2021

Trends in the biological functions and medical applications of extracellular vesicles and analogues.

Acta Pharm Sin B 2021 Aug 10;11(8):2114-2135. Epub 2021 Mar 10.

State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.

Natural extracellular vesicles (EVs) play important roles in many life processes such as in the intermolecular transfer of substances and genetic information exchanges. Investigating the origins and working mechanisms of natural EVs may provide an understanding of life activities, especially regarding the occurrence and development of diseases. Additionally, due to their vesicular structure, EVs (in small molecules, nucleic acids, proteins, etc. Read More

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Recent Advances in Photosensitizers as Multifunctional Theranostic Agents for Imaging-Guided Photodynamic Therapy of Cancer.

Theranostics 2021 26;11(18):9054-9088. Epub 2021 Aug 26.

Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, South Africa.

In recent years tremendous effort has been invested in the field of cancer diagnosis and treatment with an overall goal of improving cancer management, therapeutic outcome, patient survival, and quality of life. Photodynamic Therapy (PDT), which works on the principle of light-induced activation of photosensitizers (PS) leading to Reactive Oxygen Species (ROS) mediated cancer cell killing has received increased attention as a promising alternative to overcome several limitations of conventional cancer therapies. Compared to conventional therapies, PDT offers the advantages of selectivity, minimal invasiveness, localized treatment, and spatio-temporal control which minimizes the overall therapeutic side effects and can be repeated as needed without interfering with other treatments and inducing treatment resistance. Read More

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Targeting of promising transmembrane proteins for diagnosis and treatment of pancreatic ductal adenocarcinoma.

Theranostics 2021 25;11(18):9022-9037. Epub 2021 Aug 25.

Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, the Netherlands.

Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal types of cancer due to the relatively late diagnosis and the limited therapeutic options. Current treatment regimens mainly comprise the cytotoxic agents gemcitabine and FOLFIRINOX. These compounds have shown limited efficacy and severe side effects, highlighting the necessity for earlier detection and the development of more effective, and better-tolerated treatments. Read More

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A CNN-based method to reconstruct 3-D spine surfaces from US images in vivo.

Med Image Anal 2021 Sep 1;74:102221. Epub 2021 Sep 1.

Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USA. Electronic address:

Three-dimensional (3-D) reconstruction of the spine surface is of strong clinical relevance for the diagnosis and prognosis of spine disorders and intra-operative image guidance. In this paper, we report a new technique to reconstruct lumbar spine surfaces in 3-D from non-invasive ultrasound (US) images acquired in free-hand mode. US images randomly sampled from in vivo scans of 9 rabbits were used to train a U-net convolutional neural network (CNN). Read More

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September 2021

Tumor organoids for cancer research and personalized medicine.

Cancer Biol Med 2021 Sep 14. Epub 2021 Sep 14.

Translational Cancer Research Center, Peking University First Hospital, Beijing 100034, China.

Organoids are three-dimensional culture systems generated from embryonic stem cells, induced pluripotent stem cells, and adult stem cells. They are capable of cell proliferation, differentiation, and self-renewal. Upon stimulation by signal factors and/or growth factors, organoids self-assemble to replicate the morphological and structural characteristics of the corresponding organs. Read More

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September 2021

Applications of nDATA for screening, quantitation, and identification of pesticide residues in fruits and vegetables using UHPLC/ESI Q-Orbitrap all ion fragmentation and data independent acquisition.

J Mass Spectrom 2021 Sep;56(9):e4783

ThermoFisher Scientific, San Jose, California, USA.

High sample throughput and effective multiresidue methods for screening, quantitation, and identification are desired for the analysis of a large number of pesticides in routine monitoring programs for food safety. This study was designed to explore the use of an UHPLC/ESI Q-Orbitrap nontarget data acquisition for target analysis (nDATA) workflow for screening 655 pesticides and quantifying a small group of 46 most likely incurred pesticide residues in fruits and vegetables in a single analysis. High-resolution mass spectrometers such as the Q-Orbitrap offer unique applications for pesticide analysis using full MS scan with data independent acquisition (DIA) or all ion fragmentation (AIF) scan. Read More

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September 2021

Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine.

Commun Biol 2021 Sep 13;4(1):1067. Epub 2021 Sep 13.

Personalised Oncology Division, The Walter and Eliza Hall Institute of Medial Research, Parkville, Victoria, 3052, Australia.

Cell embedment into a solid support matrix is considered essential for the culture of intestinal epithelial organoids and tumoroids, but this technique presents challenges that impede scalable culture expansion, experimental manipulation, high-throughput screening and diagnostic applications. We have developed a low-viscosity matrix (LVM) suspension culture method that enables efficient establishment and propagation of organoids and tumoroids from the human large intestine. Organoids and tumoroids cultured in LVM suspension recapitulate the morphological development observed in solid matrices, with tumoroids reflecting the histological features and genetic heterogeneity of primary colorectal cancers. Read More

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September 2021

Applications of Metabolomics to Precision Nutrition.

Lifestyle Genom 2021 Sep 8:1-9. Epub 2021 Sep 8.

Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.

Background: For thousands of years, disabilities due to nutrient deficiencies have plagued humanity. Rickets, scurvy, anemia, stunted growth, blindness, and mental handicaps due to nutrient deficiencies affected up to 1/10 of the world's population prior to 1900. The discovery of essential amino acids, vitamins, and minerals, in the early 1900s, led to a fundamental change in our understanding of food and a revolution in human health. Read More

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September 2021

Recent advances in polymeric nanostructured ion selective membranes for biomedical applications.

Talanta 2021 Dec 19;235:122815. Epub 2021 Aug 19.

School of Engineering, Macquarie University, Sydney, NSW, 2109, Australia. Electronic address:

Nano structured ion-selective membranes (ISMs) are very attractive materials for a wide range of sensing and ion separation applications. The present review focuses on the design principles of various ISMs; nanostructured and ionophore/ion acceptor doped ISMs, and their use in biomedical engineering. Applications of ISMs in the biomedical field have been well-known for more than half a century in potentiometric analysis of biological fluids and pharmaceutical products. Read More

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December 2021

Engineering entropy-driven based multiple signal amplification strategy for visualized assay of miRNA by naked eye.

Talanta 2021 Dec 16;235:122810. Epub 2021 Aug 16.

Department of Oncology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, China. Electronic address:

MicroRNAs (miRNAs) are currently recognized as novel biomarkers for cancer early diagnosis, therapy selection, and progression monitoring. Herein, we developed an ultrasensitive and label-free homogeneous colorimetric strategy for miRNA detection based on engineering entropy-driven amplification (EDA) coupled with nicking enzyme-assisted AuNP aggregation. In our design, the target miRNA could specifically trigger the EDA recycling process. Read More

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December 2021

Convolutional neural network with group theory and random selection particle swarm optimizer for enhancing cancer image classification.

Math Biosci Eng 2021 Jun;18(5):5573-5591

Department of Computer and Information Science, Faculty of Science and Technology, University of Macau, Macau 999078, China.

As an epitome of deep learning, convolutional neural network (CNN) has shown its advantages in solving many real-world problems. Successful CNN applications on medical prognosis and diagnosis have been achieved in recent years. Their common goal is to recognize the insights from the subtle details from medical images by building a suitable CNN model with maximum accuracy and minimum error. Read More

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A high-throughput screening of metal-organic framework based membranes for biogas upgrading.

Faraday Discuss 2021 Sep 13. Epub 2021 Sep 13.

School of Chemistry, University of Nottingham, Nottingham, NG7 2RD, UK.

Applications of biomethane as a source of renewable energy and transport fuel rely heavily on successful implementation of purification methods capable of removing undesirable impurities from biogas and increasing its calorific content. Metal-organic frameworks (MOFs) are competitive candidates for biogas upgrading due to a versatile range of attractive physical and chemical properties which can be utilised in membrane materials. In this work, we present a high-throughput computational screening methodology for efficient identification of MOF structures with promising gas separation performance. Read More

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September 2021

A new minisatellite VNTR marker, Pscp1, discovered for the identification of opium poppy.

Forensic Sci Int Genet 2021 Aug 20;55:102581. Epub 2021 Aug 20.

Forensic DNA Division, National Forensic Service, Wonju 26460, Republic of Korea. Electronic address:

Opium poppy, a member of the Papaveraceae family, is an ancient herbaceous plant and well-known medical resource in the pharmaceutical industry. However, opium poppies are grown worldwide for producing illicit drugs, significantly increasing the incidence of narcotic drug abuse. Since the narcotic poppy has not yet been genetically investigated, we characterized a novel variable number tandem repeat (VNTR) marker of forensically important poppy species based on the genetic analysis of 164 samples collected from two locations spanning the Jeolla province and Jeju island of South Korea. Read More

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Preparation of doxorubicin-loaded porous iron [email protected] polydopamine nanocomposites for MR imaging and synergistic photothermal-chemotherapy of cancer.

Colloids Surf B Biointerfaces 2021 Sep 7;208:112107. Epub 2021 Sep 7.

College of Medical Engineering, Xinxiang Medical University, Xinxiang, Henan, 453003, China. Electronic address:

Recently, the development of biosafe nanocomposites with integrated diagnosis and therapeutic modality is received great attention in anti-cancer drug delivery. In this sturdy, we developed a multifunctional [email protected] nanocomposite that combines the functions of magnetic resonance (MR) imaging, photothermal therapy (PTT) and chemotherapy into one single nanoprobe. The spherical and uniform-sized porous iron oxide nanoparticles (PION) were synthesized via a simple solvothermal method. Read More

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September 2021

Enzymatic characterization of D-lactate dehydrogenase and application in alanine aminotransferase activity assay kit.

Bioengineered 2021 Dec;12(1):6459-6471

Department of Research, Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Ningbo, China.

D-lactate dehydrogenase (D-LDH) is widely used for the clinical detection of alanine aminotransferase (ALT) activity. It is a key enzyme in ALT detection kits, and its enzymatic properties directly determine sensitivity and accuracy of such kits. In this study, D-lactate dehydrogenase (WP_011543503, LDH) coding sequence derived from was obtained from the NCBI database by gene mining. Read More

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December 2021

Highly Efficient NaGdF:Ce/Tb Nanoscintillator with Reduced Afterglow and Light Scattering for High-Resolution X-ray Imaging.

ACS Appl Mater Interfaces 2021 Sep 13. Epub 2021 Sep 13.

Institute for Lasers, Photonics, and Biophotonics and Department of Chemistry, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.

Scintillation-based X-ray excited optical luminescence (XEOL) imaging shows great potential applications in the fields of industrial security inspection and medical diagnosis. It is still a great challenge to achieve scintillators simultaneously with low toxicity, high stability, strong XEOL intensity, and weak afterglow as well as simple device processibility with weak light scattering. Herein, we introduce ethylenediaminetetraacetate (EDTA)-capped NaGdF:10Ce/18Tb nanoparticles (NPs) as a highly sensitive nanoscintillator, which meets all of the abovementioned challenges. Read More

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September 2021

Tailored Biosensors for Drug Screening, Efficacy Assessment, and Toxicity Evaluation.

ACS Sens 2021 Sep 13. Epub 2021 Sep 13.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.

Biosensors have been flourishing in the field of drug discovery with pronounced developments in the past few years. They facilitate the screening and discovery of innovative drugs. However, there is still a lack of critical reviews that compare the merits and shortcomings of these biosensors from a pharmaceutical point of view. Read More

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September 2021

Nanozymes in Point-of-Care Diagnosis: An Emerging Futuristic Approach for Biosensing.

Nanomicro Lett 2021 Sep 13;13(1):193. Epub 2021 Sep 13.

School of Biological Sciences, Queen's University Belfast, Belfast, UK.

Nanomaterial-based artificial enzymes (or nanozymes) have attracted great attention in the past few years owing to their capability not only to mimic functionality but also to overcome the inherent drawbacks of the natural enzymes. Numerous advantages of nanozymes such as diverse enzyme-mimicking activities, low cost, high stability, robustness, unique surface chemistry, and ease of surface tunability and biocompatibility have allowed their integration in a wide range of biosensing applications. Several metal, metal oxide, metal-organic framework-based nanozymes have been exploited for the development of biosensing systems, which present the potential for point-of-care analysis. Read More

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September 2021

Chemical analysis of the human brain by imaging mass spectrometry.

Analyst 2021 Sep 13;146(18):5451-5473. Epub 2021 Sep 13.

Department of Chemistry, Indian Institute of Science Education and Research Tirupati, Tirupati 517507, India.

Analysis of the chemical makeup of the brain enables a deeper understanding of several neurological processes. Molecular imaging that deciphers the spatial distribution of neurochemicals with high specificity and sensitivity is an exciting avenue in this aspect. The past two decades have witnessed a significant surge of mass spectrometry imaging (MSI) that can simultaneously map the distribution of hundreds to thousands of biomolecules in the tissue specimen at a fairly high resolution, which is otherwise beyond the scope of other molecular imaging techniques. Read More

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September 2021

Rapid Characterization of Genetic Parts with Cell-free Systems.

J Vis Exp 2021 Aug 30(174). Epub 2021 Aug 30.

US Army Combat Capabilities Development Command Chemical Biological Center;

Characterizing and cataloging genetic parts are critical to the design of useful genetic circuits. Having well-characterized parts allows for the fine-tuning of genetic circuits, such that their function results in predictable outcomes. With the growth of synthetic biology as a field, there has been an explosion of genetic circuits that have been implemented in microbes to execute functions pertaining to sensing, metabolic alteration, and cellular computing. Read More

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[Clinical monitoring of multiple sclerosis patients by means of digital technology, a field in the midst of a revolution].

Rev Neurol 2021 Sep;73(6):210-218

Hospital Universitari Vall d'Hebron-UAB, Barcelona, España.

Introduction: Despite the great advances that have occurred in the diagnosis and treatment of ? multiple sclerosis (MS), few changes have taken place in terms of clinical monitoring. The lack of time and space in clinical practice limits the assessment of invisible symptoms and certain motor symptoms as manual dexterity and walking ability, which have a clear impact on the patient functional situation.

Objective: To review the potential role of technological tools in the clinical monitoring of MS patients. Read More

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September 2021

Optical-Property-Enhancing Novel Near-Infrared Active Niosome Nanoformulation for Deep-Tissue Bioimaging.

ACS Omega 2021 Sep 25;6(35):22616-22624. Epub 2021 Aug 25.

Department of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Indore 453552 Madhya Pradesh, India.

Indocyanine green (ICG) is a clinically approved near-infrared (NIR) contrast agent used in medical diagnosis. However, ICG has not been used to its fullest for biomedical imaging applications due to its low fluorescence quantum yield, aqueous instability, concentration-dependent aggregation, and photo and thermal degradations, leading to quenching of its fluorescence emission. In the present study, a nanosized niosomal formulation, ICGNiosomes (ICGNios), is fabricated to encapsulate and protect ICG from degradation. Read More

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September 2021

Non-coding RNAs in melanoma: Biological functions and potential clinical applications.

Authors:
Qiu Peng Jia Wang

Mol Ther Oncolytics 2021 Sep 4;22:219-231. Epub 2021 Jun 4.

Department of Immunology, Changzhi Medical College, Changzhi, Shanxi 046000 China.

Malignant melanoma (MM) is a malignant tumor that originates from melanocytes and has a high mortality rate. Therefore, early diagnosis and treatment are very important for survival. So far, the exact molecular mechanism leading to the occurrence of melanoma, especially the molecular metastatic mechanism, remains largely unknown. Read More

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September 2021

Progress and application of circulating tumor cells in non-small cell lung cancer.

Mol Ther Oncolytics 2021 Sep 19;22:72-84. Epub 2021 May 19.

Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.

Non-small cell lung cancer (NSCLC) has the highest morbidity and mortality worldwide among malignant tumors. NSCLC is a great threat to health and well-being. Biopsy is the gold standard to diagnose lung cancer, but traditional biopsy methods cannot fully reflect the true condition of tumors. Read More

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September 2021

Exosomes and Atherogenesis.

Front Cardiovasc Med 2021 26;8:738031. Epub 2021 Aug 26.

Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China.

Myocardial infarction and ischemic stroke are the leading causes of mortality worldwide. Atherosclerosis is their common pathological foundation. It is known that atherosclerosis is characterized by endothelial activation/injury, accumulation of inflammatory immune cells and lipid-rich foam cells, followed by the development of atherosclerotic plaque. Read More

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