2,555 results match your criteria sce assay

Treatment of Ni-EDTA containing wastewater by electrochemical degradation using Ti self-doped TiO nanotube arrays anode.

Chemosphere 2021 Sep 3;278:130465. Epub 2021 Apr 3.

Research Center for Eco-environmental Engineering, Dongguan University of Technology, Dongguan, 523808, China. Electronic address:

Ethylene diamine tetraacetic acid (EDTA) could form stable complexes with nickel due to its strong chelation. Ni-EDTA has significant impacts on human health because of its acute toxicity and low biodegradability, thus some appropriate approaches are required for its removal. In this research, a Ti self-doped TiO nanotube arrays electrode (ECR-TiO NTA) was prepared and employed in electrochemical degradation of Ni-EDTA. Read More

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September 2021

Baseline F-FDG PET/CT quantitative parameters as prognostic factors in esophageal squamous cell cancer.

Rev Esp Med Nucl Imagen Mol 2021 Jun 1. Epub 2021 Jun 1.

Servicio de Medicina Nuclear, Hospital Universitario de Badajoz, Badajoz, España.

Aim: To determine the utility of F-FDG PET/CT quantitative parameters as prognostic factors for the response to neoadjuvant treatment, progression-free survival (PFS) and cancer-specific survival (CSS) in patients with esophageal squamous cell carcinoma (SCC).

Material And Methods: Thirty patients (29 men) diagnosed with SCC were retrospectively evaluated over a 6-year interval. Metabolic parameters were determined: maximum SUV (SUVmax), mean SUV (SUVmed), metabolic tumor volume (MTV) and total lesion glycolysis (TLG) from baseline PET/CT study. Read More

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Sulfone-containing Conjugated Polyimide 2D Nanosheets for Efficient Water Oxidation.

Chem Asian J 2021 May 31. Epub 2021 May 31.

Center of Research Excellence in Nanotechnology, King Fahd University of Petroleum & Minerals (KFUPM), Dhahran, 31261, Saudi Arabia) (Abuzar Khan.

Water oxidation is a bottleneck in artificial photosynthesis that impedes its practicality for solar energy conversion and utilization. It is highly desired to significantly improve the efficacy of the existing catalysts or to rationally design new catalysts with improved performance. We report a novel conjugated and sulfone containing polyimide as a metal-free photocatalyst synthesized via a two-step method: (i) synthesis of precursor poly(amic acid) (PAA) (ii) solvothermal synthesis of polyimide through thermal imidization. Read More

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Agallochin P, a new diterpene from Vietnamese mangrove L.

Nat Prod Res 2021 May 25:1-6. Epub 2021 May 25.

School of Chemical Engineering (SCE), Hanoi University of Science & Technology (HUST), Hanoi, Vietnam.

A new diterpene () along with eight known compounds (-) were isolated from leaves. The structure and relative configuration of new compound were established on the basis of spectroscopic data analysis and confirmed by NMR chemical shifts calculation with DP4+ probability. Cytotoxicity of the isolated compounds were also evaluated. Read More

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A review of naturalistic driving study surrogates and surrogate indicator viability within the context of different road geometries.

Accid Anal Prev 2021 Jul 18;157:106185. Epub 2021 May 18.

Centre for Accident Research & Road Safety, Queensland University of Technology, Brisbane, QLD, Australia.

Advancements in data collection and processing methods have produced large databases containing high quality vehicular data. Despite this, conventional vehicle-vehicle collisions remain difficult to identify due to their rarity. Therefore, there is a need to identify potential collisions given the introduction of these new data collection methods. Read More

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mRNA Expressions of Candidate Genes in Gestational Day 16 Conceptus and Corresponding Endometrium in Repeat Breeder Dairy Cows with Suboptimal Uterine Environment Following Transfer of Different Quality Day 7 Embryos.

Animals (Basel) 2021 Apr 11;11(4). Epub 2021 Apr 11.

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA.

Effect of the gestational day (GD) 7 embryo quality grade (QG) and subclinical endometritis (SCE) on mRNA and protein expressions of candidate genes [Interferon-τ (), IFN stimulated genes (), Peroxisome proliferator activated receptors (), Retinoid X receptors ( and ), and Mucin-1 ()] in GD16 conceptus and corresponding endometrium were evaluated. After screening of performance records ( = 2389) and selection of repeat breeders ( = 681), cows with SCE (≥6% polymorphonuclear neutrophils-PMN; = 180) and no-SCE (<6%PMN; = 180) received GD7 embryos of different QGs. Based on GD16 conceptus recovery, cows with SCE ( = 30) and No- SCE ( = 30) that received GD7 embryos QG1 (good, = 20), 2 (fair, = 20), and 3 (poor, = 20) were included for gene analysis. Read More

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Continuous twin screw granulation: Impact of microcrystalline cellulose batch-to-batch variability during granulation and drying - A QbD approach.

Int J Pharm X 2021 Dec 19;3:100077. Epub 2021 Mar 19.

Laboratory of Pharmaceutical Technology, Department of Pharmaceutics, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, Belgium.

Despite significant advances in the research domain of continuous twin screw granulation, limited information is currently available on the impact of raw material properties, especially considering batch-to-batch variability. The importance of raw material variability and subsequent mitigation of the impact of this variability on the manufacturing process and drug product was recently stressed in the Draft Guidance for Industry on Quality Considerations for Continuous Manufacturing by the U.S. Read More

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December 2021

CuBiO Synthesis, Characterization, and Application in Sensitive Amperometric/Voltammetric Detection of Amoxicillin in Aqueous Solutions.

Nanomaterials (Basel) 2021 Mar 15;11(3). Epub 2021 Mar 15.

Faculty of Industrial Chemistry and Environmental Engineering, Politehnica University Timisoara, Piata Victoriei No. 2, 300006 Timisoara, Romania.

CuBiO synthesized by thermolysis of a new Bi(III)-Cu(II) oxalate coordination compound, namely BiCu(CO)·0.25HO, was tested through its integration within carbon nanofiber paste electrode, namely CuBi/carbon nanofiber (CNF), for the electrochemical detection of amoxicillin (AMX) in the aqueous solution. Thermal analysis and IR spectroscopy were used to characterize a CuBiO precursor to optimize the synthesis conditions. Read More

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Compare and contrast: Detecting mammographic soft-tissue lesions with C-Net.

Med Image Anal 2021 Jul 4;71:101999. Epub 2021 Mar 4.

Medical Imaging Center, Nanjing Jinling Hospital Clinical School, Medical College, Nanjing University, Nanjing 210002, China. Electronic address:

Detecting breast soft-tissue lesions including masses, structural distortions and asymmetries is of great importance due to the high risk leading to breast cancer. Most existing deep learning based approaches detect lesions with only unilateral images. However, multi-view mammogram images provide highly related and complementary information which helps to make the clinical analysis more comprehensive and reliable. Read More

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Electrochemical Production of Formic Acid from CO with Cetyltrimethylammonium Bromide-Assisted Copper-Based Catalysts.

ChemSusChem 2021 Apr 18;14(8):1962-1969. Epub 2021 Mar 18.

Division of Energy Storage, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, P.R. China.

The electrochemical reduction of CO (ERC) to valuable chemicals has attracted extensive attention. However, the relatively low selectivity and efficiency of the reaction remain challenges. In this study, Cu electrodes derived from Cu O with predominant (111) facets are synthesized by cetyltrimethylammonium bromide-assisted preparation. Read More

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HPLC-MS/MS-Mediated Analysis of the Pharmacokinetics, Bioavailability, and Tissue Distribution of Schisandrol B in Rats.

Int J Anal Chem 2021 24;2021:8862291. Epub 2021 Feb 24.

Anhui Provincial Center for Drug Clinical Evaluation, Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China.

Schisandrol B, a lignan isolated from dried fruits, has been shown to exhibit hepatoprotective, cardioprotective, renoprotective, and memory-enhancing properties. This study sought to design a sensitive and efficient HPLC-MS/MS approach to measuring Schisandrol B levels in rat plasma and tissues in order to assess the pharmacokinetics, oral bioavailability, and tissue distributions of this compound . For this analysis, bifendate was chosen as an internal standard (IS). Read More

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February 2021

Transcriptome Analysis of the Anti-TGFβ Effect of Fruit Extract and Schisandrin B in A7r5 Vascular Smooth Muscle Cells.

Life (Basel) 2021 Feb 20;11(2). Epub 2021 Feb 20.

Department of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Korea.

fruit extract (SCE) has been used as a traditional medicine for treating vascular diseases. However, little is known about how SCE and schisandrin B (SchB) affect transcriptional output-a crucial factor for shaping the fibrotic responses of the transforming growth factor β (TGFβ) signaling pathways in in vascular smooth muscle cells (VSMC). In this study, to assess the pharmacological effect of SCE and SchB on TGFβ-induced transcriptional output, we performed DNA microarray experiments in A7r5 VSMCs. Read More

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February 2021

Effects of topical TGF-β1, TGF-β2, ATX, and LPA on IOP elevation and regulation of the conventional aqueous humor outflow pathway.

Mol Vis 2021 20;27:61-77. Epub 2021 Jan 20.

Department of Ophthalmology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Purpose: The effects of aqueous mediators possibly increasing the outflow resistance, transforming growth factor-β1 (TGF-β1), TGF-β2, autotaxin (ATX), and lysophosphatidic acid (LPA) on human trabecular meshwork (hTM) cells and monkey Schlemm's canal endothelial (SCE) cells were characterized and compared, and the effects of intracameral application of these mediators on intraocular (IOP) elevation were also examined.

Methods: Cells were treated with TGF-β1, TGF-β2, ATX, LPA, or vehicle, and mRNA and protein expression levels of α-SMA, COL1A1, fibronectin, β-catenin, and ZO-1 were examined with real-time quantitative PCR (RT-qPCR) or immunofluorescence analyses or both. The permeability of cell monolayers was measured by determining the transendothelial electrical resistance (TEER) or with the fluorescein isothiocyanate (FITC)-dextran permeability assay. Read More

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January 2021

A novel method to simultaneously record spinal cord electrophysiology and electroencephalography signals.

Neuroimage 2021 05 19;232:117892. Epub 2021 Feb 19.

Department of Pain Management, The State Key Clinical Specialty in Pain Medicine, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China; CAS Key Laboratory of Mental Health, Institute of Psychology, Beijing, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

The brain and the spinal cord together make up the central nervous system (CNS). The functions of the human brain have been the focus of neuroscience research for a long time. However, the spinal cord is largely ignored, and the functional interaction of these two parts of the CNS is only partly understood. Read More

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Comparison of the Safety and Efficacy of Valveless and Standard Insufflation During Robotic Partial Nephrectomy: A Prospective, Randomized, Multi-institutional Trial.

Urology 2021 Feb 10. Epub 2021 Feb 10.

Hackensack University Medical Center, Hackensack, NJ; Hackensack Meridian School of Medicine at Seton Hall University, Hackensack, NJ. Electronic address:

Objective: To use a randomized, prospective, multi-institutional study to compare the safety and efficacy of conventional insufflation (CIS) and valveless insufflation (AirSeal Insufflation - AIS) at the conventional pressure of 15 mm Hg in robot-assisted partial nephrectomy - a surgery where AIS has gained popularity for maintaining visualization despite suction. This study was also powered to evaluate the effect of decreasing pneumoperitoneum by 20% in the valveless system.

Materials And Methods: Three high-volume institutions randomized subjects into CIS 15, AIS 15, and AIS 12 mm Hg cohorts. Read More

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February 2021

Photonic Crystal of Polystyrene Nanomembrane: Signal Amplification and Low Triggered Potential Electrochemiluminescence for Tetracycline Detection.

Anal Chem 2021 02 28;93(5):2959-2967. Epub 2021 Jan 28.

Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.

In this work, a low triggered potential electrochemiluminescence strategy based on gold-filled photonic crystals (GPCs) electrodes composed of photonic crystals self-assembled with polystyrene spheres and gold nanoparticles embedded in the gaps of the photonic crystals was proposed. The GPCs electrodes served as the detection platform to bind antigen, and Ru(bpy)-COOH as a luminophore was labeled on the antibody (Ab). Then, Ru(bpy)-COOH/Ab was connected to the immobilized antigen on the surface of the photonic crystals by the immunoreaction to avoid direct contact with the gold nanoparticles surface. Read More

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February 2021

Genotoxicity induced in vitro by water-soluble indoor PM fractions in relation to heavy metal concentrations.

Environ Monit Assess 2021 Jan 23;193(2):82. Epub 2021 Jan 23.

Department of Genetics, Faculty of Medicine, Democritus University of Thrace, 68100, Alexandroupolis, Greece.

The aim of the present study was to examine the genotoxicity induced by water-soluble fractions of particulate matter (PM) and its potential relation with heavy metals. For this purpose, the genotoxicity induced on human peripheral lymphocytes by water-soluble PM (particles with aerodynamic diameter ≤ 2.5 μm) collected from the indoor air of various workplaces in Greece (n = 20), was examined by the Sister Chromatid Exchange (SCE) induction assay and assessed in relation to the concentrations of the heavy metals Cu, Pb, Mn, Ni, Co, Zn, Cr, and Cd. Read More

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January 2021

Day 7 embryo quality and suboptimal uterine environment influence morphometry of Day 16 conceptus in dairy cows.

Theriogenology 2021 Mar 13;163:10-17. Epub 2021 Jan 13.

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164, USA.

The objectives of this study were to investigate the effect of Day 7 embryo quality and subclinical endometritis (SCE) in repeat breeder recipient cows on morphometry of Day 16 embryo and to determine the association of %PMN, serum progesterone and Day 16 conceptus length. Holstein dairy cows that failed to conceive at least 3 times, (parity, 3 and 4; body condition score, 3 to 3.5 out of 5) with subclinical endometritis (n = 180; SCE, >6% PMN on endometrial cytology) or without subclinical endometritis (n = 180; No-SCE, ≤ 6% PMN) were selected. Read More

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Unique Host Matrix to Disperse Pd Nanoparticles for Electrochemical Sensing of Morin: Sustainable Engineering Approach.

ACS Biomater Sci Eng 2020 09 5;6(9):5264-5273. Epub 2020 Aug 5.

Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bengaluru 560029, India.

Biomass-based carbon nanospheres derived from (commonly called "Touch-me-not") smeared on carbon fiber paper have been used as a host matrix for electrochemical deposition of palladium nanoparticles. The physicochemical characterization of modified electrodes was performed by field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction spectroscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy techniques. Cyclic voltammetry and electrochemical impedance spectroscopy were used to study the electroanalytical properties of the electrodes. Read More

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September 2020

The maize SUMO conjugating enzyme ZmSCE1b protects plants from paraquat toxicity.

Ecotoxicol Environ Saf 2021 Mar 12;211:111909. Epub 2021 Jan 12.

College of Life Science, Henan Agricultural University, Zhengzhou 450002, PR China; Synergetic Innovation Center of Henan Grain Crops and State Key Laboratory of Wheat & Maize Crop Science, Zhengzhou 450002, PR China. Electronic address:

Paraquat (PQ) herbicide causes damage to green plant tissues by inducing the production of toxic reactive oxygen species (ROS). SUMOylation is an important post-translational modification that enables plants to defend against multiple stresses. However, it is still unknown whether the SUMOylation is involved in PQ resistance response in crops. Read More

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Nickel sulfide nanoworm network architecture as a binder-free high-performance non-enzymatic glucose sensor.

Mikrochim Acta 2021 Jan 8;188(2):34. Epub 2021 Jan 8.

Key Laboratory of Polymer Material Advanced Manufacturing Technology of Shandong Provincial, College of Electromechanical Engineering, College of Sino-German Science and Technology, Qingdao University of Science and Technology, Qingdao, 266061, Shandong, People's Republic of China.

Nickel sulfide nanoworm (NiS NW) network architecture was directly grown on the poly (3,4-ethylenedioxythiophene)-reduced graphene oxide hybrid films (PEDOT-rGO HFs) modified on glassy carbon electrode (GCE), acting as a binder-free sensor for high-performance non-enzymatic glucose monitoring. The sensor exhibited the satisfactory sensitivity (2123 μA mM cm), wide linear range (15~9105 μM), low detection limit (0.48 μM), and rapid response time (< 1. Read More

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January 2021

Comparative biochemical profiles, utero-ovarian function, and fertility of the postpartum buffalo with and without subclinical endometritis.

Trop Anim Health Prod 2021 Jan 5;53(1):73. Epub 2021 Jan 5.

ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India.

In postpartum buffaloes, the process of uterine involution and changes in blood metabolic profile has not been studied in relation to development of subclinical endometritis (SCE). In this study, buffaloes (n = 100) approaching calving were identified. Weekly blood samples were collected on the day of calving up to 6 weeks post-calving. Read More

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January 2021

Allochthonous and Autochthonous Halothermotolerant Bioanodes From Hypersaline Sediment and Textile Wastewater: A Promising Microbial Electrochemical Process for Energy Recovery Coupled With Real Textile Wastewater Treatment.

Front Bioeng Biotechnol 2020 17;8:609446. Epub 2020 Dec 17.

Univ. Manouba, ISBST, BVBGR-LR11ES31, Biotechpole Sidi Thabet, Ariana, Tunisia.

The textile and clothing industry is the first manufacture sector in Tunisia in terms of employment and number of enterprises. It generates large volumes of textile dyeing wastewater (TDWW) containing high concentrations of saline, alkaline, and recalcitrant pollutants that could fuel tenacious and resilient electrochemically active microorganisms in bioanodes of bioelectrochemical systems. In this study, a designed hybrid bacterial halothermotolerant bioanode incorporating indigenous and exogenous bacteria from both hypersaline sediment of Chott El Djerid (HSCE) and TDWW is proposed for simultaneous treatment of real TDWW and anodic current generation under high salinity. Read More

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December 2020

Validation of the single-platform ISHAGE protocol for enumeration of CD34+ hematopoietic stem cells in umbilical cord blood in a Brazilian center.

Hematol Transfus Cell Ther 2020 Dec 4. Epub 2020 Dec 4.

Instituto Nacional de Câncer (INCA), Rio de Janeiro, RJ, Brazil.

Background: This study aims to validate the single-platform method for enumeration of CD34+ cells, by comparing the performance of two different commercial kits, as well as to evaluate the efficiency of the Accuri C6 cytometer in providing direct counts of absolute cell numbers.

Method: We evaluated 20 samples from umbilical cord blood (UCB), comparing the two different methodologies for enumeration of CD34+ cells: single and dual-platform. For the assessment of the single-platform, Procount and SCE kits were used, both of which use fluorescent beads as a counting reference to obtain absolute CD34+ cells numbers. Read More

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December 2020

and evaluation of clastogenicity of second-line antitubercular drug loaded PLGA nanoparticles.

Hum Exp Toxicol 2021 Jul 20;40(7):1064-1073. Epub 2020 Dec 20.

Department of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.

Sustained release nanoformulations of second line antitubercular drugs levofloxacin and ethionamide had shown promise in pharmacokinetics and acute and sub-acute toxicity studies. The present study evaluated the clastogenicity potential of the nanoformulations of these antitubercular agents. Clastogenicity was evaluated by (a) in vitro micronucleus assay (b) in vivo micronucleus assay in Swiss albino mice and (c) sister chromatid exchange (SCE) in CHO cell lines. Read More

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Double strand breaks (DSBs) as indicators of genomic instability in PATRR-mediated translocations.

Hum Mol Genet 2021 02;29(24):3872-3881

Division of Human Genetics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.

Genomic instability contributes to a variety of potentially damaging conditions, including DNA-based rearrangements. Breakage in the form of double strand breaks (DSBs) increases the likelihood of DNA damage, mutations and translocations. Certain human DNA regions are known to be involved in recurrent translocations, such as the palindrome-mediated rearrangements that have been identified at the breakpoints of several recurrent constitutional translocations: t(11;22)(q23;q11), t(17;22)(q11;q11) and t(8;22) (q24;q11). Read More

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February 2021

Nitrogen-doped porous carbon derived from digested sludge for electrochemical reduction of carbon dioxide to formate.

Sci Total Environ 2021 Mar 11;759:143575. Epub 2020 Nov 11.

School of Environmental and Safety Engineering, Changzhou University, 1 Gehu Road, Changzhou, Jiangsu Province 214500, China.

Carbon-based materials have been applied as cost-effective electrocatalysts to reduce carbon dioxide (CO) into valuable chemicals. Here, an environment-friendly method is proposed to obtain nitrogen-doped porous carbons (NPCs) from digested sludge, which is an abundant waste product from sewage treatment plants. The materials were used as a metal-free electrocatalyst for electrochemical reduction of CO to formate. Read More

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3D microstructural study of selachimorph enameloid evolution.

J Struct Biol 2021 03 19;213(1):107664. Epub 2020 Nov 19.

Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR5023 LEHNA, F-69622, Villeurbanne, France.

Enameloid, the hyper-mineralized tissue covering shark teeth is a complex structure resulting from both ameloblast and odontoblast activity. The way these two types of cells interact to set up this tissue is not fully understood and results in the formation of subunits in the enameloid: the Single Crystallite Enameloid (SCE) and the Bundled Crystallite Enameloid (BCE). Using the Focused Ion Beam Nanotomography (FIB-nt), 3D images were produced to assess the relationship between the SCE and BCE of one fossil and one recent neoselachian shark teeth. Read More

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ClusterM: a scalable algorithm for computational prediction of conserved protein complexes across multiple protein interaction networks.

BMC Genomics 2020 Nov 18;21(Suppl 10):615. Epub 2020 Nov 18.

Department of Electrical and Computer Engineering, Texas A&M University, College Station, 77843, TX, USA.

Background: The current computational methods on identifying conserved protein complexes across multiple Protein-Protein Interaction (PPI) networks suffer from the lack of explicit modeling of the desired topological properties within conserved protein complexes as well as their scalability.

Results: To overcome those issues, we propose a scalable algorithm-ClusterM-for identifying conserved protein complexes across multiple PPI networks through the integration of network topology and protein sequence similarity information. ClusterM overcomes the computational barrier that existed in previous methods, where the complexity escalates exponentially when handling an increasing number of PPI networks; and it is able to detect conserved protein complexes with both topological separability and cohesive protein sequence conservation. Read More

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November 2020

Cytogenetic effects of antidiabetic drug metformin.

Drug Chem Toxicol 2020 Nov 8:1-8. Epub 2020 Nov 8.

Department of Biology, Science Faculty, Gazi University, Ankara, 06500, Turkey.

Metformin (MET) is the first-choice antidiabetic drug for type 2 treatment. In this study, the genotoxic potential of MET was evaluated by using chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) assays in human peripheral lymphocytes as well as comet assay in isolated lymphocytes. Human lymphocytes were treated with different concentrations of MET (12. Read More

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November 2020