99,936 results match your criteria sars-cov-2 covid-19


Can commercial automated immunoassays be utilized to predict neutralizing antibodies after SARS-CoV-2 infection? A comparative study between three different assays.

Front Biosci (Landmark Ed) 2021 Jul;26(7):198-206

Biomedical Research Center, Qatar University, 2713 Doha, Qatar.

: High-throughput assays that can infer neutralizing activity against SARS-CoV-2 are of great importance for assessing the immunity induced by natural infection and COVID-19 vaccines. We aimed to evaluate the performance and degree of correlation of three fully automated anti-SARS-CoV-2 immunoassays with neutralization activity using a surrogate virus-neutralizing test (sVNT) from GenScript, targeting the receptor-binding domain. : 110 sera collected from PCR-confirmed asymptomatic COVID-19 individuals were tested for neutralizing antibodies (nAbs) using the sVNT. Read More

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A Review of the Cardiac and Cardiovascular Effects of COVID-19 in Adults and Children.

Tex Heart Inst J 2021 Jul;48(3)

Division of Critical Care, Department of Pediatrics, Baylor College of Medicine, Texas Children's Hospital, Houston, Texas.

Symptomatic coronavirus disease 2019 (COVID-19) typically affects the respiratory system but can involve the cardiovascular system. Cardiac complications of COVID-19 can result directly from myocarditis or indirectly from numerous other mechanisms. Differentiating between primary and secondary cardiovascular involvement-our focus in this review-may help to identify the long-term effects of COVID-19 on the heart in adults and children. Read More

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Elementary Laboratory Assays as Biomarkers of Ageing: Support for Treatment of COVID-19?

Gerontology 2021 Aug 2:1-14. Epub 2021 Aug 2.

Kantonsspital Graubünden, Chur, Switzerland.

Youth, working age and the elderly: On a timeline, chronological age (CA) and biological age (BA) may dissociate; nosological entities manifest themselves at different BAs. In determining which disease corresponds to a given age decade, statistical registries of causes of death are unreliable and this does not change with SARS CoV-2 infection. Beyond adolescence, ageing metrics involve estimations of changes in fitness, including prediction models to estimate the number of remaining years left to live. Read More

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Potential inhibitors of the main protease of SARS-CoV-2 and modulators of arachidonic acid pathway: Non-steroidal anti-inflammatory drugs against COVID-19.

Comput Biol Med 2021 Jul 29;136:104686. Epub 2021 Jul 29.

Department of Medicinal Chemistry, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland. Electronic address:

The main protease of SARS-CoV-2 is one of the key targets to develop and design antiviral drugs. There is no general agreement on the use of non-steroidal anti-inflammatory drugs (NSAIDs) in COVID-19. In this study, we investigated NSAIDs as potential inhibitors for chymotrypsin-like protease (3CLpro) and the main protease of the SARS-CoV-2 to find out the best candidates, which can act as potent inhibitors against the main protease. Read More

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Identifying molecular insight of synergistic complexities for SARS-CoV-2 infection with pre-existing type 2 diabetes.

Comput Biol Med 2021 Jul 23;136:104668. Epub 2021 Jul 23.

Healthy Ageing Theme, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia; WHO Collaborating Centre on eHealth, UNSW Digital Health, School of Public Health and Community Medicine, Faculty of Medicine, UNSW Sydney, NSW, 2052, Australia. Electronic address:

The ongoing COVID-19 outbreak, caused by SARS-CoV-2, has posed a massive threat to global public health, especially to people with underlying health conditions. Type 2 diabetes (T2D) is lethal comorbidity of COVID-19. However, its pathogenetic link remains unclear. Read More

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Going to work with COVID-19 symptoms among non-sanitary (or socio-sanitary) workers: an issue of social inequality.

Public Health 2021 Jul 5;198:6-8. Epub 2021 Jul 5.

Union Institute of Work, Environment and Health (ISTAS), Reference Centre on Work Organisation and Health, Barcelona, Spain.

Objecives: To describe the characteristics of the workers of activity sectors other than sanitary and socio-sanitary, who go to work with COVID-19 symptoms (GWC19S) during the lockdown or first phase of the lockdown de-escalation in Spain.

Study Design: An observational cross-sectional study based on a convenience sample selected from the COTS online survey.

Methods: A cross-sectional study based on a sample of n = 9601 workers. Read More

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Evaluation of S-RBD and high specificity ACE-2-binding antibodies on SARS-CoV-2 patients after six months from infection.

Int Immunopharmacol 2021 Jul 27;99:108013. Epub 2021 Jul 27.

Department of Laboratory Medicine, Tor Vergata University Hospital, Rome, Italy; Department of Experimental Medicine, University of Tor Vergata, Rome, Italy.

The antibody response to SARS-CoV-2 has not yet fully defined, but the availability of sensitive and specific serological assays is crucial to observe the presence of specific antibodies against the human receptor binding domain (S-RBD) and high specificity ACE-2-binding antibodies or neutralizing antibodies (NT) in response to vaccines. Indeed, these peculiar antibodies should prevent viral interaction between RBD and Angiotensin-Converting Enzyme 2 (ACE2) receptor, located on surface of host cells. In this study, 72 samples from 37 hospitalized COVID-19 patients and 35 not-hospitalized patients were analyzed longitudinally. Read More

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The study of recovery rates of COVID-19 olfactory and gustatory dysfunctions requires psychophysical evaluations.

Am J Otolaryngol 2021 Jul 29;42(6):103168. Epub 2021 Jul 29.

COVID-19 Task Force of the Young-Otolaryngologists of the International Federations of Oto-rhino-laryngological Societies (YO-IFOS), Paris, France; Maxillofacial Surgery Operative Unit, Department of Medical, Surgical and Experimental Sciences, University of Sassari, Italy; Biomedical Science PhD School, Biomedical Science Department, University of Sassari, Italy.

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Prediction of two novel overlapping ORFs in the genome of SARS-CoV-2.

Authors:
Angelo Pavesi

Virology 2021 Jul 28;562:149-157. Epub 2021 Jul 28.

Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area Delle Scienze 23/A, I-43124, Parma, Italy. Electronic address:

Six candidate overlapping genes have been detected in SARS-CoV-2, yet current methods struggle to detect overlapping genes that recently originated. However, such genes might encode proteins beneficial to the virus, and provide a model system to understand gene birth. To complement existing detection methods, I first demonstrated that selection pressure to avoid stop codons in alternative reading frames is a driving force in the origin and retention of overlapping genes. Read More

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Potential protective role of the anti-PD-1 blockade against SARS-CoV-2 infection.

Biomed Pharmacother 2021 Jul 28;142:111957. Epub 2021 Jul 28.

Lab of Chemical Biology and Molecular Drug Design, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China; Institute of Drug Development & Chemical Biology, Zhejiang University of Technology, Hangzhou 310014, China. Electronic address:

The outbreak of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Wuhan, China, in December 2019, and its global dissemination became the coronavirus disease 2019 (COVID-19) pandemic declared by the World Health Organization (WHO) on 11 March 2020. In patients undergoing immunotherapy, the effect and path of viral infection remain uncertain. In addition, viral-infected mice and humans show T-cell exhaustion, which is identified after infection with SARS-CoV-2. Read More

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Advances in the computational landscape for repurposed drugs against COVID-19.

Drug Discov Today 2021 Jul 30. Epub 2021 Jul 30.

Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Electronic address:

The COVID-19 pandemic has caused millions of deaths and massive societal distress worldwide. Therapeutic solutions are urgently needed but de novo drug development remains a lengthy process. One promising alternative is computational drug repurposing, which enables the prioritization of existing compounds through fast in silico analyses. Read More

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Absence of SARS-CoV-2 antibodies in pre-pandemic plasma from children and adults in Vietnam.

Int J Infect Dis 2021 Jul 30. Epub 2021 Jul 30.

Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. Electronic address:

We tested pre-pandemic (2015-2019) plasma samples from 148 Vietnamese children, and 100 Vietnamese adults at high risk of zoonotic infections, for antibodies against SARS-CoV-2 nucleocapsid and spike proteins. None was positive. The data thus demonstrated that there was no evidence of prior serological cross-reactivity with SARS-CoV-2 that might explain the low numbers of COVID-19 in Vietnam. Read More

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Psychiatric and neuropsychiatric sequelae of COVID-19 - a systematic review.

Brain Behav Immun 2021 Jul 30. Epub 2021 Jul 30.

Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, 8000 Aarhus C, Denmark. Electronic address:

It has become evident that coronavirus disease 2019 (COVID-19) has a multi-organ pathology that includes the brain and nervous system. Several studies have also reported acute psychiatric symptoms in COVID-19 patients. An increasing number of studies are suggesting that psychiatric deficits may persist after recovery from the primary infection. Read More

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Antimicrobial stewardship in the ICU in COVID times: the known unknowns.

Int J Antimicrob Agents 2021 Jul 30:106409. Epub 2021 Jul 30.

Division of Infectious Diseases, American University of Beirut Medical Center, Lebanon.

Since the start of the COVID-19 pandemic, there has been concern about the concomitant rise of antimicrobial resistance (AMR). While bacterial co-infections seem rare in COVID-19 patients admitted to hospital wards and ICUs, an increase in empirical antibiotic use has been described. In the ICU setting, where antibiotics are already abundantly -and often inappropriately- prescribed, the need for an ICU specific Antimicrobial Stewardship Program (ASP) is widely advocated. Read More

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Origin, phylogeny, variability and epitope conservation of SARS-CoV-2 worldwide.

Virus Res 2021 Jul 30:198526. Epub 2021 Jul 30.

Molecular Microbiology and Biotechnology Department, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.

The coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses innumerous challenges, like understanding what triggered the emergence of this new human virus, how this RNA virus is evolving or how the variability of viral genome may impact the primary structure of proteins that are targets for vaccine. We analyzed 19471 SARS-CoV-2 genomes available at the GISAID database from all over the world and 3335 genomes of other Coronoviridae family members available at GenBank, collecting SARS-CoV-2 high-quality genomes and distinct Coronoviridae family genomes. Additionally, we analyzed 199,984 spike glycoprotein sequences. Read More

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Evaluation of low-cost viral concentration methods in wastewaters: implications for SARS-CoV-2 pandemic surveillances.

J Virol Methods 2021 Jul 30:114249. Epub 2021 Jul 30.

Laboratory of Molecular Virology, CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, Salto CP: 50.000, Uruguay. Electronic address:

In the pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) many strategies have been performed in order to control viral spread in the population and known the real-time situation about the number of infected persons. In this sense, Wastewater Based Epidemiology (WBE) has been applied as an excellent tool to evaluate the virus circulation in a population. In order to obtain reliable results, three low-cost viral concentration methods were evaluated in this study, polyethylene glycol (PEG) precipitation, skimmed milk flocculation (SM) and Aluminum polychloride flocculation, for Pseudomonas aeruginosa bacteriophage PP7 as a surrogate for non-enveloped viruses and Bovine Coronavirus (BCoV) as a surrogate for enveloped virus, with emphasis for SARS- CoV-2. Read More

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Liver injury following SARS-CoV-2 vaccination: a multicenter case series.

J Hepatol 2021 Jul 30. Epub 2021 Jul 30.

Division of Gastroenterology and Hepatology, Feinberg School of Medicine at Northwestern University, Chicago, IL.

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Scleroderma renal crisis following mRNA vaccination against SARS-CoV-2.

Kidney Int 2021 Jul 30. Epub 2021 Jul 30.

AP-HP, Hôpitaux Universitaires Henri Mondor, Service de Néphrologie et Transplantation, Centre de Référence Maladie Rare « Syndrome Néphrotique Idiopathique », Fédération Hospitalo-Universitaire TRUE, Créteil, France; Université Paris Est Créteil (UPEC), Institut National de la Santé et de la Recherche Médicale (INSERM) U955, Institut Mondor de Recherche Biomédicale (IMRB), Equipe 21, Créteil, France. Electronic address:

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Abnormality in the NK cell population is prolonged in severe COVID-19 patients.

J Allergy Clin Immunol 2021 Jul 30. Epub 2021 Jul 30.

Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea. Electronic address:

Background: Our understanding of adaptive immune responses in COVID-19 patients is rapidly evolving, but information on the innate immune responses by natural killer (NK) cells is still insufficient.

Objective: We aimed to examine the phenotypic and functional status of NK cells and their changes during the course of mild and severe COVID-19.

Methods: We performed RNA sequencing (RNA-seq) and flow cytometric analysis of NK cells from patients with mild and severe COVID-19 at multiple time points in the course of the disease using cryopreserved peripheral blood mononuclear cells (PBMCs). Read More

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Myopericarditis following mRNA COVID-19 Vaccination in Adolescents 12 through 18 Years of Age.

J Pediatr 2021 Jul 30. Epub 2021 Jul 30.

Beaumont Children's Hospital, 3601 W 13 Mile Road, Royal Oak, Michigan, 48073 and Oakland University William Beaumont School of Medicine, 586 Pioneer Drive, Rochester, Michigan 48309.

Objectives: To characterize the clinical course and outcomes of children who developed probable myopericarditis after vaccination with the Pfizer- BioNTech (BNT162b2) COVID-19 mRNA vaccine.

Study Design: A cross-sectional study of 32 children, aged 12 through 18 years, diagnosed with probable myopericarditis following COVID-19 mRNA vaccination as per the CDC criteria for myopericarditis at 9 US centers between May 10, 2021 and June 20, 2021. We retrospectively collected the following data: demographics, SARS-CoV-2 virus detection or serologic testing, clinical manifestations, laboratory test results, imaging study results, treatment and time to resolutions of symptoms. Read More

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"Bucket brigade" using lysine residues in RNA-dependent RNA polymerase of SARS-CoV-2.

Biophys J 2021 Jul 30. Epub 2021 Jul 30.

Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan; Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan; Department of Structural Molecular Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Aichi 444-8787, Japan. Electronic address:

The RNA-dependent RNA polymerase (RdRp) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a promising drug target for coronavirus disease 2019 (COVID-19) because it plays the most important role in the replication of the RNA genome. Nucleotide analogs such as remdesivir and favipiravir are thought to interfere with the RNA replication by RdRp. More specifically, they are expected to compete with nucleoside triphosphates, such as adenosine triphosphate (ATP). Read More

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The mechanisms of smell loss after SARS-CoV-2 infection.

Authors:
Richard L Doty

Lancet Neurol 2021 Jul 30. Epub 2021 Jul 30.

Smell and Taste Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address:

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Post-viral effects of COVID-19 in the olfactory system and their implications.

Lancet Neurol 2021 Jul 30. Epub 2021 Jul 30.

Department of Microbiology and Immunology, University of Iowa, Iowa City, IA, USA.

Background: The mechanisms by which any upper respiratory virus, including SARS-CoV-2, impairs chemosensory function are not known. COVID-19 is frequently associated with olfactory dysfunction after viral infection, which provides a research opportunity to evaluate the natural course of this neurological finding. Clinical trials and prospective and histological studies of new-onset post-viral olfactory dysfunction have been limited by small sample sizes and a paucity of advanced neuroimaging data and neuropathological samples. Read More

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Evidence of lung perfusion defects and ongoing inflammation in an adolescent with post-acute sequelae of SARS-CoV-2 infection.

Lancet Child Adolesc Health 2021 Jul 30. Epub 2021 Jul 30.

Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A Gemelli IRCCS, Rome, Italy; Global Health Research Institute, Istituto di Igiene, Università Cattolica del Sacro Cuore, Rome, Italy.

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Microstructured Surfaces for Reducing Chances of Fomite Transmission via Virus-Containing Respiratory Droplets.

ACS Nano 2021 Aug 2. Epub 2021 Aug 2.

Department of Mechanical Engineering, Changwon National University, Changwon 51140, South Korea.

Evaporation-induced particle aggregation in drying droplets is of significant importance in the prevention of pathogen transfer due to the possibility of indirect fomite transmission of the infectious virus particles. In this study, particle aggregation was directionally controlled using contact line dynamics (pinned or slipping) and geometrical gradients on microstructured surfaces by the systematic investigation of the evaporation process on sessile droplets and sprayed microdroplets laden with virus-simulant nanoparticles. Using this mechanism, we designed robust particle capture surfaces by significantly inhibiting the contact transfer of particles from fomite surfaces. Read More

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A systems-level study reveals host-targeted repurposable drugs against SARS-CoV-2 infection.

Mol Syst Biol 2021 Aug;17(8):e10239

Department of Computational and Systems Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.

Understanding the mechanism of SARS-CoV-2 infection and identifying potential therapeutics are global imperatives. Using a quantitative systems pharmacology approach, we identified a set of repurposable and investigational drugs as potential therapeutics against COVID-19. These were deduced from the gene expression signature of SARS-CoV-2-infected A549 cells screened against Connectivity Map and prioritized by network proximity analysis with respect to disease modules in the viral-host interactome. Read More

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Neuropathological findings from COVID-19 patients with neurological symptoms argue against a direct brain invasion of SARS-CoV-2: a critical systematic review.

Eur J Neurol 2021 Aug 2. Epub 2021 Aug 2.

IRCCS Mondino Foundation, Pavia, Italy.

Background: Neuropathological studies can elucidate the mechanisms of nervous system damage associated with SARS-CoV-2 infection. Despite literature on this topic is rapidly expanding, correlations between neurological symptoms and brain pathology findings in COVID-19 patients remain largely unknown.

Methods: We performed a systematic literature review on neuropathological studies in COVID-19, including 438 patients from 45 papers published by April 22, 2021. Read More

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Comparison of clinical and laboratory profile of survivors and non-survivors of SARS-CoV-2-related multisystem inflammatory syndrome of childhood in India: An observational study.

J Paediatr Child Health 2021 Aug 2. Epub 2021 Aug 2.

Department of Pediatrics, Kalawati Saran Children's Hospital and Lady Hardinge Medical college, New Delhi, India.

Aim: The SARS-CoV-2 pandemic is characterised by multiple reports of paediatric multisystem inflammatory disease or multisystem inflammatory syndrome in children (MIS-C) with Kawasaki disease-like features often complicated by myocarditis, shock and macrophage activation syndrome. Certain clinical and laboratory markers may be used to identify high risk cases.

Methods: All sequentially admitted patients hospitalised between April 2020 and October 2020, who met the WHO case definition for MIS-C were included. Read More

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The seroprevalence of severe acute respiratory syndrome coronavirus 2 in Delhi, India: a repeated population-based seroepidemiological study.

Trans R Soc Trop Med Hyg 2021 Aug 2. Epub 2021 Aug 2.

State Surveillance Unit (SSU), Directorate General Health Services, 3rd Floor, DGD Buildling, School Block, Shakarpur, East Delhi 110092.

Background: Three rounds of a repeated cross-sectional serosurvey to estimate the change in seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were conducted from August to October 2020 in the state of Delhi, India, in the general population ≥5 y of age.

Methods: The selection of participants was through a multistage sampling design from all 11 districts and 280 wards of the city-state, with multistage allocation proportional to population size. The blood samples were screened using immunoglobulin G (IgG) enzyme-linked immunosorbent assay kits. Read More

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