49 results match your criteria samples grappa


Proceedings of the 2020 GRAPPA Collaborative Research Network (CRN) Meeting.

J Rheumatol Suppl 2021 Jun;97:4-9

O. FitzGerald, MD, FRCPI, FRCP, Consultant Rheumatologist and Newman Clinical Research Professor, Conway Institute for Biomolecular Research, University College Dublin, Dublin, Ireland.

At the 2020 Group for Research and Assessment of Psoriasis and Psoriatic Arthritis (GRAPPA)-Collaborative Research Network (CRN) annual meeting, the GRAPPA-CRN group presented a pilot investigator-initiated study protocol to test electronic case report forms (eCRFs) and proposed Standardized Operating Procedures (SOPs) to evaluate biomarkers of psoriatic arthritis (PsA) associated with axial disease. The progress on 3 studies was also presented: BioDAM PsA (Biomarkers as Predictors of structural DAMage in PsA; to validate soluble biomarkers as predictors of structural damage in PsA), PreventPsA (examining the development of PsA and risk factors among patients with psoriasis and no arthritis), and PredictORPsA (Predicting Treatment respOnse in patients with eaRly PsA; in collaboration with Pfizer using samples from the Oral Psoriatic Arthritis TriaL [OPAL], to identify biomarkers of treatment response). GRAPPA-CRN funding partnerships and applications are also underway with both the Innovative Medicines Initiative (IMI) in Europe and Accelerating Medicines Partnerships (AMP) 2. Read More

View Article and Full-Text PDF

Lipocalin-type Prostaglandin D Synthase Concentration Gradients in the Cerebrospinal Fluid in Normal-tension Glaucoma Patients with Optic Nerve Sheath Compartmentation.

Eye Brain 2021 14;13:89-97. Epub 2021 Apr 14.

Department of Biomedicine, University Hospital Basel & University Basel, Basel, Switzerland.

Objective: To report on the lipocalin-type prostaglandin D synthase (L-PGDS) concentrations in the cerebrospinal fluid (CSF) of the perioptic and lumbar subarachnoid space (SAS) in patients with radiologically proven optic nerve (ON) sheath compartmentation presenting as normal-tension glaucoma (NTG).

Methods: Retrospective biochemical analysis of CSF in thirteen patients with ON sheath compartmentation presenting as NTG (four females, mean age 70±8 years). CSF was sampled from the SAS of the ON during ON sheath fenestration for ON sheath compartmentation and from the lumbar SAS at the time of lumbar puncture. Read More

View Article and Full-Text PDF

Sensorial Impact and Distribution of 3-Methyl-2,4-nonanedione in Cognacs and Spirits.

J Agric Food Chem 2021 Apr 8;69(15):4509-4517. Epub 2021 Apr 8.

Unité de recherche OEnologie, EA 4577, USC 1366 INRAE, ISVV, Univ. Bordeaux, 33882 Villenave d'Ornon cedex, France.

The aim of this study was to improve our knowledge on the chemical markers of Cognac aromas. We report results concerning the distribution and sensorial impact of 3-methyl-2,4-nonanedione (MND), a well-known compound in aged red wine, reminiscent of anise or "dried fruit", according to its concentration. We assayed first this diketone (solid-phase microextraction (SPME)-gas chromatography (GC)/mass spectrometry (MS), chemical ionization (CI)) in many Cognac samples followed by grappa, brandy, rum, whisky, vodka, and fruit spirits, and concentrations ranged from traces to 11. Read More

View Article and Full-Text PDF

Detection of 3-O-methyldopa in dried blood spots for neonatal diagnosis of aromatic L-amino-acid decarboxylase deficiency: The northeastern Italian experience.

Mol Genet Metab 2021 05 13;133(1):56-62. Epub 2021 Mar 13.

Neurology Unit, St Bassiano Hospital, Bassano del Grappa, Italy.

Objective: Aromatic L-amino acid decarboxylase (AADC) deficiency is a rare inherited autosomal recessive disorder of biogenic amine metabolism. Diagnosis requires analysis of neurotransmitter metabolites in cerebrospinal fluid, AADC enzyme activity analysis, or molecular analysis of the DDC gene. 3-O-methyldopa (3-OMD) is a key screening biomarker for AADC deficiency. Read More

View Article and Full-Text PDF

Methods for radioactivity measurements in drinking water using gamma spectrometry.

J Environ Radioact 2021 Jun 6;232:106566. Epub 2021 Mar 6.

Alto Trevigiano Servizi, Via Schiavonesca Priula, 86 Montebelluna, TV, Italy.

In this study, three methods to measure activity concentrations of radionuclides through high resolution gamma spectrometry are developed, optimized, and tested on drinking water samples. Two pre-concentration methods (partial evaporation and ion-exchange resins) were optimized for accuracy, precision, detection limits, costs, preparation, and measurements times. A new sampling method for Rn was designed and optimized to directly sample water from the tap, reducing and minimizing losses of radon during the sampling. Read More

View Article and Full-Text PDF

APIR4EMC: Autocalibrated parallel imaging reconstruction for extended multi-contrast imaging.

Magn Reson Imaging 2021 05 14;78:80-89. Epub 2021 Feb 14.

Department of Medical Informatics, Erasmus MC, Rotterdam, the Netherlands; Department of Radiology and Nuclear Medicine, Erasmus MC, Rotterdam, the Netherlands.

Purpose: To improve image quality of multi-contrast imaging with the proposed Autocalibrated Parallel Imaging Reconstruction for Extended Multi-Contrast Imaging (APIR4EMC).

Methods: APIR4EMC reconstructs multi-contrast images in an autocalibrated parallel imaging reconstruction framework by adding contrasts as virtual coils. Compensation of signal evolution along the echo train of different contrasts is performed to improve signal prediction for missing samples. Read More

View Article and Full-Text PDF

Proceedings of the 2020 GRAPPA Collaborative Research Network (CRN) Meeting.

J Rheumatol 2021 Feb 15. Epub 2021 Feb 15.

As part of the supplement series GRAPPA 2020, this report was reviewed internally and approved by the Guest Editors for integrity, accuracy, and consistency with scientific and ethical standards. C. Stober, MBChB, MRCP, PhD, MSc, Department of Medicine, University of Cambridge, Cambridge, UK; D.R. Jadon, MBBCh, MRCP, PhD, University of Cambridge, Cambridge, UK; A.W. Armstrong, MD, PhD, Professor of Dermatology, Associate Dean for Clinical Research, Director of Clinical Research Support, Southern California Clinical and Translational Science Institute (SC CTSI), Vice Chair Director, Clinical Trials and Outcomes Research Director, Psoriasis Program, Department of Dermatology, Keck School of Medicine at USC, University of Southern California, Los Angeles, California, USA; V. Chandran, MD, DM, PhD, Department of Medicine, Division of Rheumatology, University of Toronto, Toronto Western Hospital, Krembil Research Institute, Toronto, Ontario, Canada; M. de Wit, PhD, GRAPPA Patient Research Partner, Department of Medical Humanities, Amsterdam University Medical Centre, Amsterdam, the Netherlands; P.S. Helliwell, MD, PhD Professor of Clinical Rheumatology, Leeds Institute of Rheumatic and Musculoskeletal Medicine, University of Leeds, NIHR Leeds Biomedical Research Centre, Leeds, UK; P.J. Mease, MD, Rheumatology Research, Swedish Medical Center/ Providence St. Joseph Health and University of Washington School of Medicine, Seattle, Washington, USA; A. Ogdie, MD, MSCE, Associate Professor of Medicine and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA; D. O'Sullivan, BE, GRAPPA Patient Research Partner, Our Lady's Hospice & Care Services, Rheumatic & Musculoskeletal Disease Unit, Dublin, Ireland; S.R. Pennington, PhD, School of Medicine, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland; T. Löve, MD, PhD, Faculty of Medicine, University of Iceland, and Department of Research, Landspitali University Hospital, Reykjavik, Iceland; A. Cauli, MD, PhD, Rheumatology Unit, University Clinic and AOU of Cagliari, Monserrato, Italy; L. van Mens, MD, PhD, Amsterdam University Medical Centers/University of Amsterdam, Department of Clinical Immunology and Rheumatology Amsterdam, Infection & Immunity Institute, Amsterdam, the Netherlands; R. Waxman, MPH, Leeds Institute of Rheumatic and Musculoskeletal Medicine, University of Leeds, Harehills Lane, Leeds, and NIHR Leeds Biomedical Research Centre, Leeds, UK; J.U. Scher, MD, Department of Medicine, NYU Grossman School of Medicine, New York, New York, USA; A. Barton, MBChB, MSc, PhD, FRCP, Centre for Musculoskeletal Research, The University of Manchester and NIHR Manchester Biomedical Research Centre, Manchester University NHS Trust, Manchester, UK; C.T. Ritchlin, MD, MPH, Professor of Medicine, Division of Allergy, Immunology and Rheumatology, University of Rochester Medical Center, Rochester, New York, USA; O. FitzGerald, MD, FRCPI, FRCP, Consultant Rheumatologist and Newman Clinical Research Professor, Conway Institute for Biomolecular Research, University College Dublin, Dublin, Ireland. AA has received research funding from Boehringer Ingelheim/Parexel, Bristol Myers Squibb, Dermavant, Dermira, Eli Lilly, Galderma, Janssen-Ortho Inc., Kyowa Hakko Kirin, Leo Pharma, Pfizer Inc., Sanofi Genzyme, UCB Pharma; and honorariums from AbbVie, Bristol Myers Squibb, Dermavant, Eli Lilly, Janssen Pharmaceuticals Inc., Leo Pharma, Modernizing Medicine, Novartis Pharmaceuticals Corp., Ortho Dermatologics, Pfizer Inc., Regeneron Pharmaceuticals, Sanofi Genzyme, and Sun Pharma. VC has received consulting fees and/or speaking fees and/or honoraria from AbbVie, Amgen, Bristol Myers Squibb, Celgene, Eli Lilly and Company, Janssen, Novartis, Pfizer, and UCB, and his spouse is an employee of Eli Lilly and Company. PJM has received research grants, consultation fees, and/or speaker honoraria from AbbVie, Amgen, Boehringer Ingelheim, Bristol Myers Squibb, Eli Lilly, Galapagos, Gilead, GlaxoSmithKline, Janssen, Merck, Novartis, Pfizer, Sun Pharma, and UCB. JUS has received research grants and/or consulting fees from AbbVie, Amgen, Bristol Myers Squibb, Eli Lilly, Janssen, Novartis, Pfizer Inc, Sanofi, and UCB; OF has received research grants and/ or consulting fees from AbbVie, Amgen, Bristol Myers Squibb, Celgene, Eli Lilly, Janssen, Novartis, Pfizer Inc., and UCB. All other coauthors declare no conflicts of interest. Address correspondence to Prof. Oliver FitzGerald, School of Medicine, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield Dublin 4, Ireland. Email:

At the 2020 Group for Research and Assessment of Psoriasis and Psoriatic Arthritis (GRAPPA)- Collaborative Research Network (CRN) annual meeting, the GRAPPA-CRN group presented a pilot investigator-initiated study protocol to test electronic case report forms (eCRFs) and proposed Standardized Operating Procedures (SOPs) to evaluate biomarkers of psoriatic arthritis (PsA) associated with axial disease. The progress on 3 studies was also presented: BioDAM PsA (Biomarkers as Predictors of structural DAMage in PsA; to validate soluble biomarkers as predictors of structural damage in PsA), PreventPsA (examining the development of PsA and risk factors among patients with psoriasis and no arthritis), and PredictORPsA (Predicting Treatment respOnse in patients with eaRly PsA; in collaboration with Pfizer using samples from the Oral Psoriatic Arthritis TriaL [OPAL], to identify biomarkers of treatment response). GRAPPA-CRN funding partnerships and applications are also underway with both the Innovative Medicines Initiative (IMI) in Europe and Accelerating Medicines Partnerships (AMP) 2. Read More

View Article and Full-Text PDF
February 2021

Changes in volatile compounds of grape pomace distillate (Italian grappa) during one-year ageing in oak and cherry barrels.

Food Chem 2021 May 27;344:128658. Epub 2020 Nov 27.

Council for Agricultural Research and Economics - Viticulture & Oenology (CREA-VE), Viale XXVIII Aprile 26, 31015 Conegliano, TV, Italy. Electronic address:

The Italian grape pomace distillate grappa is often refined by ageing in wooden barrels. Chemical changes of the volatile profile of two samples produced from a Cabernet Sauvignon/Merlot blend and Prosecco pomace both at two ethanol contents (55% and 68%) were studied during one-year ageing in cherry and oak barriques. Grappa variety, ethanol content and barrel type strongly affected the volatiles profile of the distillates. Read More

View Article and Full-Text PDF

Comparison between a novel molecular tool and conventional methods for diagnostic classification of bacterial vaginosis: is integration of the two approaches necessary for a better evaluation?

New Microbiol 2020 Jul 13;43(3):121-126. Epub 2020 Jul 13.

Laboratory Medicine, San Bassiano Hospital, Bassano del Grappa, Italy.

The etiological cause of bacterial vaginosis (BV) is the change of the vaginal ecosystem characterized by a decrease of lactobacilli and an increase of other germs, such as Gardnerella vaginalis and Atopobium vaginae. Molecular tools have revolutionized the diagnosis of these conditions. The aim of this paper was to compare results obtained from 158 vaginal swabs collected from women aged between 18 and 59 years old and subjected to microscopic evaluation (Nugent Score), culture and to the multiparametric molecular assay Vaginitis and Vaginosis Multiplex-Tandem (MT) PCR (AU27117) - Nuclear Laser Medicine. Read More

View Article and Full-Text PDF

Characterisation of Formaggella della Valle di Scalve Cheese Produced From Cows Reared in Valley Floor Stall or in Mountain Pasture: Fatty Acids Profile and Sensory Properties.

Foods 2020 Mar 26;9(4). Epub 2020 Mar 26.

Department of Veterinary Science-University of Parma, Via del Taglio 10, I-43126 Parma, Italy.

An important problem in mountain areas is the abandonment of pasture. This trend can be combated by the valorisation of typical dairy products, such as "Formaggella della Valle di Scalve", a semi-cooked traditional cheese made from whole milk in a mountain area in Italy. The aim of the present research was to compare the fatty acid (FA) profile and the sensory properties of this cheese as manufactured under different conditions: i) from the milk of cows grazing on mountain or valley pasture or fed indoors; ii) from the milk of cows fed hay or fed silage. Read More

View Article and Full-Text PDF

Human and environmental factors affecting the activity of I and Cs in urban wastewater: A case study.

J Environ Radioact 2019 Mar 31;198:135-146. Epub 2018 Dec 31.

ETRA SpA Energia Territorio Risorse Ambientali, Largo Parolini 82, 36061, Bassano del Grappa (VI), Italy.

The analysis of radionuclides content in the waste product of urban wastewater treatment (i.e. sewage sludges) is of great concern for both the safety of workers and in a re-use perspective of the final product. Read More

View Article and Full-Text PDF

A collaborative study by the Working Group on Hemostasis and Thrombosis of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology (SIBioC) on the interference of haemolysis on five routine blood coagulation tests by evaluation of 269 paired haemolysed/non-haemolysed samples.

Biochem Med (Zagreb) 2018 Oct;28(3):030711

Section of Clinical Biochemistry, University of Verona, Verona, Italy.

Introduction: Haemolysis is the leading cause of sample rejection in laboratory haemostasis. Most studies focused on artificially haemolysed samples. The aim of this study was a prospective assessment of spontaneous haemolysis on haemostasis tests, by comparing results of haemolysed (H) new, non-haemolysed (NH) specimens, collected within 4hrs. Read More

View Article and Full-Text PDF
October 2018

Accelerated silent echo-planar imaging.

Magn Reson Imaging 2019 01 18;55:81-85. Epub 2018 Sep 18.

Imaging Centre of Excellence (ICE), Institute of Neuroscience and Psychology, College of Medical, Veterinary & Life Sciences University of Glasgow, Glasgow, United Kingdom.

Purpose: The standard approach to Echo-Planar Imaging (EPI) is to use trapezoidal readout (RO) gradients with blipped phase-encoding (PE) gradients. Sinusoidal RO gradients with constant PE gradients can reduce acoustic noise. However, this sequence, originally introduced by Mansfield et al. Read More

View Article and Full-Text PDF
January 2019

Computation of exact g-factor maps in 3D GRAPPA reconstructions.

Magn Reson Med 2019 02 6;81(2):1353-1367. Epub 2018 Sep 6.

Department of Radiology, University of Wisconsin-Madison, Madison, Wisconsin.

Purpose: To characterize the noise distributions in 3D-MRI accelerated acquisitions reconstructed with GRAPPA using an exact noise propagation analysis that operates directly in k-space.

Theory And Methods: We exploit the extensive symmetries and separability in the reconstruction steps to account for the correlation between all the acquired k-space samples. Monte Carlo simulations and multi-repetition phantom experiments were conducted to test both the accuracy and feasibility of the proposed method; a high-resolution in-vivo experiment was performed to assess the applicability of our method to clinical scenarios. Read More

View Article and Full-Text PDF
February 2019

Proceedings of the 2017 GRAPPA Collaborative Research Network Meeting.

J Rheumatol Suppl 2018 Jun;94:54-61

From the Rheumatology Research Unit, Cambridge University Hospitals UK National Health Service (NHS) Foundation Trust, Cambridge; University of Leeds, Leeds; Bradford Hospitals NHS Foundation Trust, Bradford, UK; Department of Medicine, Division of Rheumatology, University of Toronto, Toronto Western Hospital, Krembil Research Institute, Toronto, Ontario, Canada; Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania; University of Southern California, Los Angeles, California; University of Utah, Salt Lake City, Utah; Division of Allergy, Immunology, and Rheumatology, University of Rochester Medical Center, Rochester, New York, USA; Our Lady's Hospice and Care Services; Newman Clinical Research Professor, Department of Rheumatology, St. Vincent's University Hospital and Conway Institute for Biomolecular Research, University College Dublin, Dublin, Ireland; Department of Medical Humanities, VU University Medical Centre, Amsterdam, the Netherlands.

The Group for Research and Assessment of Psoriasis and Psoriatic Arthritis (GRAPPA) Collaborative Research Network (CRN) is an endeavor that aims to address gaps in the knowledge of the etiopathogenesis and management of psoriatic disease by best using the large community of experienced investigators who are already collecting rich clinical phenotype data and biologic samples using validated techniques. Exemplar rheumatology and dermatology projects will inform strategies to implement the CRN, while input and funding from government organizations, charities, and industry will shape the CRN. The key immediate priorities to establish the CRN are discussed herein and include (1) strategies for building infrastructure to collect and store biosamples and associated clinical data, (2) best practices for sample collection and storage, (3) approaches to engage the GRAPPA community of investigators and industry to collaborate most effectively on shared priorities, and (4) agreement on a funding strategy. Read More

View Article and Full-Text PDF

The Microbiome in Psoriasis and Psoriatic Arthritis: Joints.

Authors:
Jose U Scher

J Rheumatol Suppl 2018 Jun;94:32-35

From the Psoriatic Arthritis Center, Division of Rheumatology, New York University Langone Health; Microbiome Center for Rheumatology and Autoimmunity (MiCRA), Division of Rheumatology, New York University School of Medicine and Hospital for Joint Diseases, New York, New York, USA.

The microbiome is a known and established immunomodulator of many inflammatory disorders, including psoriasis and psoriatic arthritis. Microbes co-evolved with their human hosts and provide them with nutritional, metabolic, and immunologic support. An accumulating body of evidence has revealed that psoriatic diseases are characterized by a state of intestinal dysbiosis, which has been linked to a decrease in beneficial commensals and fatty acids. Read More

View Article and Full-Text PDF

Dispersive liquid-liquid microextraction and gas chromatography accurate mass spectrometry for extraction and non-targeted profiling of volatile and semi-volatile compounds in grape marc distillates.

J Chromatogr A 2018 Apr 3;1546:36-45. Epub 2018 Mar 3.

Departamento de Química Analítica, Nutrición y Bromatología, Instituto de Investigación y Análisis Alimentario (IIAA), Universidade de Santiago de Compostela, Santiago de Compostela 15782, Spain.

The suitability of dispersive liquid-liquid microextraction (DLLME) and gas chromatography accurate mass spectrometry (GC-MS), based on a time-of-flight (TOF) MS analyzer and using electron ionization (EI), for the characterization of volatile and semi-volatile profiles of grape marc distillates (grappa) are evaluated. DLLME conditions are optimized with a selection of compounds, from different chemical families, present in the distillate spirit. Under final working conditions, 2. Read More

View Article and Full-Text PDF

May car washing represent a risk for Legionella infection?

Ann Ig 2018 Jan-Feb;30(1):57-65

Department of Cardiac, Thoracic and Vascular Sciences, Hygiene and Public Health Unit, University of Padua, Padua, Italy.

Background: Legionella is a ubiquitous Gram-negative bacterium naturally found in aquatic environments. It can pose a health problem when it grows and spreads in man-made water systems. Legionella pneumophila is the most common cause of Legionnaires' disease nowadays, a community-acquired pneumonia with pulmonary symptoms and chest radiography no different from any other form of infectious pneumonia. Read More

View Article and Full-Text PDF
October 2019

Newborn screening for lysosomal storage disorders by tandem mass spectrometry in North East Italy.

J Inherit Metab Dis 2018 03 15;41(2):209-219. Epub 2017 Nov 15.

Neurological Unit, St. Bassiano Hospital, Via dei Lotti, 40, 36061, Bassano del Grappa, Italy.

Background: Lysosomal storage diseases (LSDs) are inborn errors of metabolism resulting from 50 different inherited disorders. The increasing availability of treatments and the importance of early intervention have stimulated newborn screening (NBS) to diagnose LSDs and permit early intervention to prevent irreversible impairment or severe disability. We present our experience screening newborns in North East Italy to identify neonates with Mucopolysaccharidosis type I (MPS I) and Pompe, Fabry, and Gaucher diseases. Read More

View Article and Full-Text PDF

Clinical spectrum and IgG subclass analysis of anti-myelin oligodendrocyte glycoprotein antibody-associated syndromes: a multicenter study.

J Neurol 2017 Dec 23;264(12):2420-2430. Epub 2017 Oct 23.

Clinical Department of Neurology, Medical University of Innsbruck, Innsbruck, Austria.

Anti-myelin oligodendrocyte glycoprotein antibodies (MOG-Ab) recently emerged as a potential biomarker in patients with inflammatory demyelinating diseases of the central nervous system. We here compare the clinical and laboratory findings observed in a cohort of MOG-Ab seropositive and seronegative cases and describe IgG subclass analysis results. Consecutive serum samples referred to Verona University Neuropathology Laboratory for aquaporin-4 (AQP4)-Ab and/or MOG-Ab testing were analysed between March 2014 and May 2017. Read More

View Article and Full-Text PDF
December 2017

Exact Calculation of Noise Maps and ${g}$ -Factor in GRAPPA Using a ${k}$ -Space Analysis.

IEEE Trans Med Imaging 2018 02 9;37(2):480-490. Epub 2017 Oct 9.

Characterization of the noise distribution in magnetic resonance images has multiple applications, including quality assurance and protocol optimization. Noise characterization is particularly important in the presence of parallel imaging acceleration with multi-coil acquisitions, where the noise distribution can contain severe spatial heterogeneities. If the parallel imaging reconstruction is a linear process, an accurate noise analysis can be carried out by taking into account the correlations between all the samples involved. Read More

View Article and Full-Text PDF
February 2018

Comparing the performance of three panels rules of blood smear review criteria on an Italian multicenter evaluation.

Int J Lab Hematol 2017 Dec 3;39(6):645-652. Epub 2017 Oct 3.

Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy.

Background: The aims of this study were to compare the diagnostic accuracy of blood smear review criteria, by means of three different panel rules, those proposed by: the International Consensus Group for Hematology [41-ICGH rules], the Italian Survey [IS rules] and the Working Group on Hematology-SIBioC (WGH) consensus rules (WGH rules).

Methods: This study is based on 2707 peripheral blood (PB) samples referred for routine hematological testing to the WGH-associated laboratories displaced all over the Italian territory. The PB samples were processed on seven different hematology analyzers (HAs): Advia 2120i, XE-2100, BC-6800, ABX Pentra, XN-1000, Cell-DYN Sapphire, and DxH800, respectively. Read More

View Article and Full-Text PDF
December 2017

Skin globotriaosylceramide 3 deposits are specific to Fabry disease with classical mutations and associated with small fibre neuropathy.

PLoS One 2017 3;12(7):e0180581. Epub 2017 Jul 3.

IRCCS Institute of Neurological Sciences, Bologna, Italy.

Background: Fabry Disease (FD) is characterized by globotriaosylceramide-3 (Gb3) accumulation in several tissues and a small fibre neuropathy (SFN), however the underlying mechanisms are poorly known. This study aimed to: 1) ascertain the presence of Gb3 deposits in skin samples, by an immunofluorescence method collected from FD patients with classical GLA mutations or late-onset FD variants or GLA polymorphisms; 2) correlate skin GB3 deposits with skin innervation.

Methods: we studied 52 genetically-defined FD patients (32 with classical GLA mutations and 20 with late-onset variants or GLA polymorphisms), 15 patients with SFN associated with a specific cause and 22 healthy controls. Read More

View Article and Full-Text PDF
October 2017

Technical Note: Evaluation of pre-reconstruction interpolation methods for iterative reconstruction of radial k-space data.

Med Phys 2017 Aug 5;44(8):4025-4034. Epub 2017 Jul 5.

Department of Radiology and Imaging Sciences, University of Utah, Salt Lake City, UT, 84108, USA.

Purpose: To evaluate the use of three different pre-reconstruction interpolation methods to convert non-Cartesian k-space data to Cartesian samples such that iterative reconstructions can be performed more simply and more rapidly.

Methods: Phantom as well as cardiac perfusion radial datasets were reconstructed by four different methods. Three of the methods used pre-reconstruction interpolation once followed by a fast Fourier transform (FFT) at each iteration. Read More

View Article and Full-Text PDF

Microextraction by packed sorbent (MEPS)-UHPLC-UV: A simple and efficient method for the determination of five benzodiazepines in an alcoholic beverage.

J Pharm Biomed Anal 2016 Jun 14;125:48-53. Epub 2016 Mar 14.

University of Urbino Carlo Bo, Dept. SPeA, LC-MS Laboratory, 61029 Urbino, Italy. Electronic address:

This article describes a nano-scale method for the determination and quantification of five benzodiazepines (BDZ) in an alcoholic grappa drink (chlordiazepoxide; lorazepam; diazepam; oxazepam; medazepam). BDZ are typically used in drug-facilitated crimes (DFC) for their accessibility and synergistic effects with alcohol. Specimens collected on the crime scene must be rapidly analyzed to prove the crime, though, in most cases, a very small amount is available. Read More

View Article and Full-Text PDF

Multicenter comparison of automated procalcitonin immunoassays.

Pract Lab Med 2015 Aug 17;2:22-28. Epub 2015 Jul 17.

Laboratory of Clinical Chemistry and Hematology, Academic Hospital of Parma, Parma, Italy.

Objectives: A multicenter study to compare results of BRAHMS Kryptor PCT with those obtained using four BRAHMS-partnered procalcitonin (PCT) automated immunoassays (DiaSorin Liaison, BioMérieux Vidas, Roche Cobas E601 and Siemens Advia Centaur) and the Diazyme immunotubidimetric assay implemented on four clinical chemistry platforms (Abbott Architect c16000, Siemens Advia 2400, Roche Cobas C501 and Beckman Coulter AU5800).

Design And Methods: One hundred serum samples from in-patients with PCT values between 0.10 and 58. Read More

View Article and Full-Text PDF

Multicenter Comparison of Seven 25OH Vitamin D Automated Immunoassays.

J Med Biochem 2015 Jul 14;34(3):344-350. Epub 2015 Jul 14.

Laboratory of Clinical Chemistry and Hematology, San Bortolo Hospital, Vicenza, Italy.

Background: The measurement of 25OH vitamin D continues to grow in clinical laboratories. The aim of this multi-center study was to compare the results of seven automated commercial immunoassays with a reference HPLC technique.

Methods: One hundred and twenty consecutive outpatient serum samples were centrifuged, divided in aliquots, frozen and shipped to the participating laboratories. Read More

View Article and Full-Text PDF

Nutritional Quality of Preparations Based on Döner Kebab Sold in Two Towns of Veneto Region, Italy: Preliminary Results.

Ital J Food Saf 2015 May 9;4(2):4535. Epub 2015 Jun 9.

Department of Comparative Biomedicine and Food Science, University of Padua.

The sampling activity for this study was performed between September and October 2012. It involved seven shops in Verona, eleven in Vicenza and two in its province (Bassano del Grappa), northern Italy. The scope was to measure the values of energy and nutritional components and to identify the profile of fatty acids in a serving of ready to eat Döner Kebab. Read More

View Article and Full-Text PDF

Miniaturized multi-coil arrays for functional planar imaging with a single-sided NMR sensor.

J Magn Reson 2015 May 12;254:10-8. Epub 2015 Feb 12.

Institut für Technische und Makromolekulare Chemie (ITMC), RWTH Aachen University, Worringer Weg 1, D-52074 Aachen, Germany.

Nowadays most low-field NMR sensors, such as the single-sided Profile NMR-MOUSE®, still suffer from poor sensitivity, either resulting from low magnetic field strengths and correspondingly low NMR frequencies, or lack of sensitivity. Generally, micro-coils can improve sensitivity, but due to their small size, and thus small inductance, they are mainly used for high-field NMR. Their main application field is parallel imaging, where those coils are typically assembled to receive-only coil-arrays and increase the field-of-view. Read More

View Article and Full-Text PDF

Check-in and sorting of centrifuged serum and lithium-heparin tubes may be unsuitable using a bulk input module.

J Lab Autom 2014 Oct 27;19(5):474-7. Epub 2014 May 27.

Laboratory of Clinical Chemistry and Hematology, Academic Hospital of Parma, Parma, Italy

The aim of this study was to establish that laboratory testing may be impaired by pouring and sorting of centrifuged tubes into a bulk input module. The values of 17 analytes, including albumin, aspartate aminotransferase (AST), bilirubin, urea nitrogen, C-reactive protein (CRP), calcium, chloride, cholesterol, creatine kinase (CK), creatinine, ferritin, glucose, lactate dehydrogenase (LDH), potassium, sodium, total protein, and thyroid-stimulating hormone (TSH), were measured in 29 centrifuged serum samples collected in tubes with gel separator and paired centrifuged lithium-heparin samples collected in tubes with gel separator, with (S-BIM) or without (S-NO-BIM) being poured and sorted by a bulk input module (Inpeco, Lugano, Switzerland). In serum tubes, significant differences were observed in S-BIM values of albumin, AST, cholesterol, ferritin, glucose, LDH, potassium, and sodium compared with S-NO-BIM, with bias of LDH exceeding the total allowable error. Read More

View Article and Full-Text PDF
October 2014