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Benzo[a]pyrene diol epoxide-induced transformed cells identify the significance of hsa_circ_0051488, a ERCC1-derived circular RNA in pulmonary squamous cell carcinoma.

Mol Carcinog 2021 Jul 28. Epub 2021 Jul 28.

Department of Toxicology, School of Public Health, China Medical University, Shenyang, Liaoning, China.

ERCC1 is a gene for repairing DNA damage whose function is related to carcinogenic-induced tumorigenesis and the effectiveness of platinum therapies. Circular RNAs (circRNAs) are products of posttranscriptional regulation with pleiotropic effects on the pathogenesis of lung cancer. We aim to identify that specific circRNAs derived from ERCC1 can regulate key biological processes involved in the development of lung cancer. Read More

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Serum Reference Intervals of Fat-soluble Vitamins D and E in Wild Florida Manatees (Trichechus manatus latirostris) and Possible Implications for the Health of Manatees in Managed Care.

Ray L Ball

J Wildl Dis 2021 Jul 28. Epub 2021 Jul 28.

ZooTampa at Lowry Park, 1101 W. Sligh Avenue, Tampa, Florida 33604, USA.

Serum levels of alpha-tocopherol (vitamin E) and 25-hydroxyvitamin D (vitamin D) were measured in 25 wild Florida manatees (Trichechus manatus latirostris) from Crystal River National Wildlife Refuge, Citrus County, Florida. The samples were collected during the 2017 and 2018 winter health assessments. Reference intervals for alpha-tocopherol were 0. Read More

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rPanglaoDB: an R package to download and merge labeled single-cell RNA-seq data from the PanglaoDB database.

Bioinformatics 2021 Jul 28. Epub 2021 Jul 28.

Department of Veterinary Integrative Biosciences.

Motivation: Characterizing cells with rare molecular phenotypes is one of the promises of high throughput single-cell RNA sequencing (scRNA-seq) techniques. However, collecting enough cells with the desired molecular phenotype in a single experiment is challenging, requiring several samples preprocessing steps to filter and collect the desired cells experimentally before sequencing. Data integration of multiple public single-cell experiments stands as a solution for this problem, allowing the collection of enough cells exhibiting the desired molecular signatures. Read More

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An evaluation of diagnostic tests in a case series of suspected leptospirosis patients seen in primary care.

N Z Med J 2021 Jul 30;134(1539):33-43. Epub 2021 Jul 30.

Professor of Veterinary Public Health, Molecular Epidemiology and Public Health Laboratory, School of Veterinary Science, Massey University, Palmerston North.

Aims: This study describes 47 presentations of suspected leptospirosis in general practice in New Zealand. Our primary aim was to assess the laboratory diagnosis of leptospirosis in these patients, by comparing polymerase chain reaction (PCR) tests, microscopic agglutination test (MAT) and culture results.

Methods: Patients suspected of leptospirosis were recruited from general practices in the Waikato (n=17) and Wairoa (n=30) between August 2011 and June 2015. Read More

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Single-Sided Deafness Cochlear Implant Sound-Localization Behavior With Multiple Concurrent Sources.

Ear Hear 2021 Jul 26. Epub 2021 Jul 26.

National Military Audiology and Speech Pathology Center, Walter Reed National Military Medical Center, Bethesda, Maryland, USA Department of Otolaryngology - Head and Neck Surgery, University of Missouri School of Medicine, Columbia, Missouri, USA.

Objectives: For listeners with one deaf ear and the other ear with normal/near-normal hearing (single-sided deafness [SSD]) or moderate hearing loss (asymmetric hearing loss), cochlear implants (CIs) can improve speech understanding in noise and sound-source localization. Previous SSD-CI localization studies have used a single source with artificial sounds such as clicks or random noise. While this approach provides insights regarding the auditory cues that facilitate localization, it does not capture the complex nature of localization behavior in real-world environments. Read More

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IgE and IgG4 Repertoire in Asymptomatic HDM-Sensitized and HDM-Induced Allergic Rhinitis Patients.

Int Arch Allergy Immunol 2021 Jul 28:1-12. Epub 2021 Jul 28.

Department of Allergy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan, China.

Introduction: Asymptomatic sensitization is defined as the presence of positive skin prick test (SPT) and/or positive serum allergen-specific IgE in the absence of clinical allergic symptoms. Currently, there is no convincing explanation why some people with positive allergen tests do not show symptoms. We aimed to investigate the house dust mite (HDM)-specific IgE and IgG4 repertoire in asymptomatic HDM-sensitized subjects and HDM-induced allergic rhinitis (AR) patients. Read More

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Plasma progesterone secretion during gestation of the captive short-beaked echidna.

Reproduction 2021 Jul 1. Epub 2021 Jul 1.

S Johnston, School of Agriculture and Food Science, The University of Queensland, Gatton, Australia.

This study describes the progesterone profile during pregnancy in sexually mature female captive short-beaked echidnas (Tachyglossus aculeatus aculeatus). Echidnas were monitored daily by video surveillance to confirm key reproductive behaviour. Plasma samples were collected and pouch morphology assessed three times a week. Read More

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Application of a fast and sensitive method for the determination of contaminants of emerging concern in wastewater using a quick, easy, cheap, effective, rugged and safe-based extraction and liquid chromatography coupled to mass spectrometry.

J Chromatogr A 2021 Jul 9;1653:462396. Epub 2021 Jul 9.

CIESOL (Solar Energy Research Center), Joint Centre University of Almeria-CIEMAT, Carretera de Sacramento s/n, Almeria 04120, Spain; Department of Chemistry and Physics, University of Almeria, Carretera de Sacramento s/n, Almeria 04120, Spain.

The inefficiency of wastewater treatment plants (WWTPs) to remove contaminants of emerging concern (CECs) leads to their continuous release to the environment. Consequently, CECs are present at low concentrations in the treated wastewater (TWW), producing unpredicted and unwanted effects on living organisms as they are discharged into water receiving bodies. This work presents a fast and reliable method for the determination of CECs in TWW based on the innovative application of a QuEChERS (quick, easy, cheap, effective, rugged and safe) method for water extraction and determination by sensitive liquid chromatography coupled to quadrupole-linear ion trap tandem mass spectrometry (LC-QqLIT-MS/MS). Read More

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Microscale extraction combined with gas chromatography/mass spectrometry for the simultaneous determination of polycyclic aromatic hydrocarbons and polycyclic aromatic sulfur heterocycles in marine sediments.

J Chromatogr A 2021 Jul 14;1653:462414. Epub 2021 Jul 14.

Instituto de Química, Universidade Federal da Bahia, Salvador, BA 40170-115, Brazil; Centro Interdisciplinar de Energia e Ambiente - CIEnAm, Universidade Federal da Bahia, Salvador, BA 40170-115, Brazil. Electronic address:

This paper describes a novel method based on an ultrasound-assisted extraction microscale device (UAE-MSD) for the rapid and simultaneous determination of polycyclic aromatic hydrocarbons (PAH) and polycyclic aromatic sulfur heterocycles (PASH) in marine sediments. Solvent extraction conditions were optimized by applying a simplex-centroid mixture design. Optimum conditions were used to validate and determine the concentrations of 17 PAH and 7 PASH. Read More

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Separation of monomeric and dimeric phenolic compounds in lignosulphonate lignin on different stationary phases using ultrahigh-performance supercritical fluid chromatography.

J Chromatogr A 2021 Jul 14;1653:462408. Epub 2021 Jul 14.

Lund University, Department of Chemistry, Centre for Analysis and Synthesis, P.O. Box 124, SE-22100 Lund, Sweden. Electronic address:

Lignin is a promising renewable resource and its valorization could help to reduce our dependency on fossil carbon resources. Especially the production of small molecular weight and economically valuable compounds, such as vanillin, are of interest. A good separation of the sample components is crucial for a confident identification of compounds in complex sample mixtures using for instance mass spectrometry. Read More

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The emotional responses of women when terminating a pregnancy for medical reasons: A scoping review.

Midwifery 2021 Jul 14;103:103095. Epub 2021 Jul 14.

Department of Nursing, University Rovira i Virgili. Avinguda Catalunya, 35, Postal Code 43002, Tarragona, Spain.

Background: In most countries of the world the only basis for considering a termination of pregnancy is for medical reasons. Depending on the circumstances and determinants of each case, the emotional responses to this event vary greatly. The aim of this study is to map the emotional responses of women when their pregnancy is terminated for medical reasons. Read More

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Validation and implementation of a direct RT-qPCR method for rapid screening of SARS-CoV-2 infection by using non-invasive saliva samples.

Int J Infect Dis 2021 Jul 25. Epub 2021 Jul 25.

Institut de Recerca Sant Joan de Deu, Esplugues de Llobregat, Barcelona, Spain; Department of Medicine, Universitat Internacional de Catalunya, Barcelona, Spain; Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain; Hospital Sant Joan de Deu, Universitat de Barcelona, Esplugues de Llobregat, Barcelona, Spain. Electronic address:

Objective: To validate and implement an optimised screening method for detection of SARS-CoV-2 RNA combining use of self-collected raw saliva samples, single-step heat-treated virus inactivation and RNA extraction, and direct RT-qPCR.

Methods: 3-phased study conducted in Barcelona (Spain) in June-October, 2020, including: i) analytical validation against standard RT-qPCR in saliva samples; ii) diagnostic validation against standard RT-qPCR using paired saliva-nasopharyngeal samples obtained from asymptomatic teenagers and adults in a sports academy; and iii) pilot screening of asymptomatic health workers in a tertiary hospital.

Results: Phase i) Detection yield of the new method was comparable to that of standard RT-qPCR. Read More

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Probing machine-learning classifiers using noise, bubbles, and reverse correlation.

J Neurosci Methods 2021 Jul 25:109297. Epub 2021 Jul 25.

Laboratoire des systèmes perceptifs, Département d'études cognitives, École normale supérieure, PSL University, CNRS, 75005 Paris, France.

Background: Many scientific fields now use machine-learning tools to assist with complex classification tasks. In neuroscience, automatic classifiers may be useful to diagnose medical images, monitor electrophysiological signals, or decode perceptual and cognitive states from neural signals. However, such tools often remain black-boxes: they lack interpretability. Read More

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Comparison of Self-collected Mouth Gargle with Deep-throat Saliva Samples for the diagnosis of COVID-19: Mouth gargle for diagnosis of COVID-19.

J Infect 2021 Jul 25. Epub 2021 Jul 25.

Department of Microbiology, The Chinese University of Hong Kong, Hong Kong SAR, China; Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong SAR, China. Electronic address:

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Immune Markers Are Associated with Cognitive Performance in a Multiethnic Cohort: the Northern Manhattan Study.

Brain Behav Immun 2021 Jul 25. Epub 2021 Jul 25.

Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, USA.

Objective: To determine whether immune protein panels add significant information to correlates of cognition.

Background: Immune mechanisms in vascular cognitive aging are incompletely characterized.

Design/methods: A subsample of the prospective Northern Manhattan Study underwent detailed neuropsychological testing. Read More

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The Cancer SENESCopedia: A delineation of cancer cell senescence.

Cell Rep 2021 Jul;36(4):109441

Division of Molecular Carcinogenesis, Oncode Institute, Netherlands Cancer Institute, Plesmanlaan 121, 1066 Amsterdam, the Netherlands. Electronic address:

Cellular senescence is characterized as a stable proliferation arrest that can be triggered by multiple stresses. Most knowledge about senescent cells is obtained from studies in primary cells. However, senescence features may be different in cancer cells, since the pathways that are involved in senescence induction are often deregulated in cancer. Read More

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Geographic differences in gut microbiota composition impact susceptibility to enteric infection.

Cell Rep 2021 Jul;36(4):109457

Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA. Electronic address:

Large-scale studies of human gut microbiomes have revealed broad differences in composition across geographically distinct populations. Yet, studies examining impacts of microbiome composition on various health outcomes typically focus on single populations, posing the question of whether compositional differences between populations translate into differences in susceptibility. Using germ-free mice humanized with microbiome samples from 30 donors representing three countries, we observe robust differences in susceptibility to Citrobacter rodentium, a model for enteropathogenic Escherichia coli infections, according to geographic origin. Read More

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Dimensionality reduction by UMAP reinforces sample heterogeneity analysis in bulk transcriptomic data.

Cell Rep 2021 Jul;36(4):109442

The University of Queensland Diamantina Institute, Faculty of Medicine, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia; Shandong Artificial Intelligence Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China; Laboratory of Immunology for Environment and Health, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China. Electronic address:

Transcriptomic analysis plays a key role in biomedical research. Linear dimensionality reduction methods, especially principal-component analysis (PCA), are widely used in detecting sample-to-sample heterogeneity, while recently developed non-linear methods, such as t-distributed stochastic neighbor embedding (t-SNE) and uniform manifold approximation and projection (UMAP), can efficiently cluster heterogeneous samples in single-cell RNA sequencing analysis. Yet, the application of t-SNE and UMAP in bulk transcriptomic analysis and comparison with conventional methods have not been achieved. Read More

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Waterborne outbreak in a rural area in Greece during the COVID-19 pandemic: contribution of community pharmacies.

Rural Remote Health 2021 Jul 28;21(3):6630. Epub 2021 Jul 28.

Directorate of Research, Studies and Documentation, National Public Health Organization, 3-5 Agrafon St, Athens 15123, Greece

Introduction: On 15 June 2020, the National Public Health Organization was informed about the identification of two cases of Escherichia coli Ο157 infection in a small town in the Peloponnese Region in Greece and we suspected an outbreak.

Methods: We asked the local pharmacist to assist us to verify the outbreak by providing the daily number of over-the-counter anti-diarrheal drugs sold from 20 May 2020 onwards. The pharmacist asked customers with gastroenteritis to submit stool samples at the local hospital. Read More

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mWISE: An Algorithm for Context-Based Annotation of Liquid Chromatography-Mass Spectrometry Features through Diffusion in Graphs.

Anal Chem 2021 Jul 28. Epub 2021 Jul 28.

B2SLab, Departament d'Enginyeria de Sistemes, Automàtica i Informàtica Industrial, Universitat Politècnica de Catalunya, Av. Diagonal 647, 08028 Barcelona, Spain.

Untargeted metabolomics using liquid chromatography coupled to mass spectrometry (LC-MS) allows the detection of thousands of metabolites in biological samples. However, LC-MS data annotation is still considered a major bottleneck in the metabolomics pipeline since only a small fraction of the metabolites present in the sample can be annotated with the required confidence level. Here, we introduce mWISE (metabolomics wise inference of speck entities), an R package for context-based annotation of LC-MS data. Read More

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Evaluation of Spin Columns for Human Plasma Depletion to Facilitate MS-Based Proteomics Analysis of Plasma.

J Proteome Res 2021 Jul 28. Epub 2021 Jul 28.

Cancer Proteomics Mass Spectrometry, Scilifelab, Department of Oncology and Pathology, Karolinska Institutet, SE-141 86 Stockholm, Sweden.

High abundant protein depletion is a common strategy applied to increase analytical depth in global plasma proteomics experiment setups. The standard strategies for depletion of the highest abundant proteins currently rely on multiple-use HPLC columns or multiple-use spin columns. Here we evaluate the performance of spin columns for plasma depletion and show that the single-use spin reduces handling time by allowing parallelization and is easily adapted to a nonspecialized lab environment without reducing the high plasma proteome coverage and reproducibility. Read More

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Covid-19 Breakthrough Infections in Vaccinated Health Care Workers.

N Engl J Med 2021 Jul 28. Epub 2021 Jul 28.

From the Infection Prevention and Control Unit (M.B., T.G., C.C., E.G.L., C.R., I.T., M.Z., G.R.-Y.), the Department of Clinical Microbiology (S.A.), General Management (Y.K.), and the Central Virology Laboratory, Public Health Authority, Ministry of Health (Y.L., M.M., V.I., N.Z.), Sheba Medical Center Tel Hashomer, Ramat Gan, Sackler School of Medicine, Tel Aviv University, Tel Aviv (T.G., Y.L., M.M., E.G.L., Y.K., G.R.-Y.), and the Laboratory Wing, Asaf Harofe Medical Center, Be'er Ya'akov (A.B.-C.) - all in Israel; St. George's School of Medicine of London and Faculty of Medicine, University of Nicosia, Nicosia, Cyprus (M.B.); and Harvard T.H. Chan School of Public Health, Boston (M.L.).

Background: Despite the high efficacy of the BNT162b2 messenger RNA vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), rare breakthrough infections have been reported, including infections among health care workers. Data are needed to characterize these infections and define correlates of breakthrough and infectivity.

Methods: At the largest medical center in Israel, we identified breakthrough infections by performing extensive evaluations of health care workers who were symptomatic (including mild symptoms) or had known infection exposure. Read More

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Different patterns in root and soil fungal diversity drive plant productivity of the desert truffle Terfezia claveryi in plantation.

Environ Microbiol 2021 Jul 28. Epub 2021 Jul 28.

Departamento Biología Vegetal, Facultad de Biología, CEIR Campus Mare Nostrum (CMN, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.

The desert truffle Terfezia claveryi is one of the few mycorrhizal fungi currently in cultivation in semiarid and arid areas. Agroclimatic parameters seem to affect its annual yield, but there is no information on the influence of biotic factors. In this study, fungal diversity was analyzed by high-throughput sequencing of the ITS2 rDNA region from soil and root samples to compare productive and non-productive mycorrhizal plants in a 4-years-old plantation (Murcia, Spain). Read More

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4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis.

Prostate 2021 Jul 28. Epub 2021 Jul 28.

Department of Urology, The First Affiliated Hospital of Anhui Medical University, Institute of Urology, and Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China.

Background: Hyaluronan (HA), an extracellular matrix component, accumulates in most chronic inflammatory tissues. Here, we studied the impact of HA on the pathogenesis of chronic prostatitis.

Materials And Methods: First, we sorted demographic characteristics and peripheral blood serum samples from patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) to assess the relationship between the levels of HA in peripheral blood serum and the severity of inflammation in patients. Read More

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Expression and prognostic significance of vascular endothelial growth factor-A (VEGF-A) and its receptor in canine prostate cancer.

Prostate 2021 Jul 28. Epub 2021 Jul 28.

Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University-UNESP, Botucatu, Sao Paulo, Brazil.

Background: Vascular endothelial growth factor-A (VEGF-A) and its receptor, VEGF receptor-2 (VEGFR-2), represent a complex family of angiogenic molecules consisting of different ligands and receptors. Due to the importance of VEGF-A/VEGFR-2 signaling in tumor proliferation and angiogenesis, this study aimed to evaluate the protein and gene expression levels of VEGF-A and VEGFR-2 in canine prostate cancer (PC).

Methods: We analyzed VEGF-A and VEGFR-2 expression in 87 PC samples by immunohistochemistry and quantitative-polymerase chain reaction. Read More

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A Multi-center Genome-wide Association Study of Cervical Dystonia.

Mov Disord 2021 Jul 28. Epub 2021 Jul 28.

Department of Neurology, University of Lübeck, Lübeck, Germany.

Background: Several monogenic causes for isolated dystonia have been identified, but they collectively account for only a small proportion of cases. Two genome-wide association studies have reported a few potential dystonia risk loci; but conclusions have been limited by small sample sizes, partial coverage of genetic variants, or poor reproducibility.

Objective: To identify robust genetic variants and loci in a large multicenter cervical dystonia cohort using a genome-wide approach. Read More

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Exploring the moderating role of gender in the relation between emotional expressivity and posttraumatic stress disorder symptom severity among Black trauma-exposed college students at a historically Black university.

J Clin Psychol 2021 Jul 28. Epub 2021 Jul 28.

University of Rhode Island, Kingston, Rhode Island, USA.

Objectives: Posttraumatic stress disorder (PTSD) is characterized in part by negative alterations of cognition or mood, including alterations in emotional expressivity, or the extent to which one outwardly displays emotions. Yet, research in this area has relied on predominantly white samples and neglected to consider the potential role of gender, despite there being demonstrated gender differences in both PTSD symptom severity and emotional expressivity, separately. The goal of the current study was to fill a critical gap in the literature by examining the moderating role of gender in the relation between PTSD symptom severity and emotional expressivity in a sample of trauma-exposed Black adults. Read More

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New Therapeutic Approach for Intestinal Fibrosis Through Inhibition of pH-Sensing Receptor GPR4.

Inflamm Bowel Dis 2021 Jul 28. Epub 2021 Jul 28.

Department of Gastroenterology and Hepatology, University Hospital Zurich, Zurich, Switzerland.

Background: Patients suffering from inflammatory bowel diseases (IBDs) express increased mucosal levels of pH-sensing receptors compared with non-IBD controls. Acidification leads to angiogenesis and extracellular matrix remodeling. We aimed to determine the expression of pH-sensing G protein-coupled receptor 4 (GPR4) in fibrotic lesions in Crohn's disease (CD) patients. Read More

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Comparison of Breath and Blood Alcohol Concentrations in a Controlled Drinking Study.

J Anal Toxicol 2021 Jul 28. Epub 2021 Jul 28.

Wisconsin Department of Transportation, Division of State Patrol, Bureau of Transportation Safety, Chemical Testing Section3502 Kinsman Blvd., Madison, WI 53704 USA.

In this work, 114 volunteers were dosed with 80-proof liquor to produce peak blood- or breath- alcohol concentration of 0.040 to 0.080 g/100mL blood or g/210L breath. Read More

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