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Single-cell Transcriptomics Uncover a Novel Role of Myeloid Cells and T-lymphocytes in the Fibrotic Microenvironment in Peyronie's Disease.

Eur Urol Focus 2021 May 4. Epub 2021 May 4.

Laboratory of Experimental Urology, Department of Development and Regeneration, KU Leuven, Leuven, Belgium; Department of Urology, University Hospitals Leuven, Leuven, Belgium.

Background: Peyronie's disease (PD) is an acquired fibrotic disease affecting the penile tunica albuginea that can lead to curvature and deformities, shortening, and erectile dysfunction. Although immunological mechanisms have been suggested for the pathophysiology of PD, these have not been investigated using single-cell transcriptomics.

Objective: To investigate the immunological signature of plaques from PD patients using immunohistochemistry (IHC) and single-cell RNA sequencing (scRNA-Seq). Read More

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Soil-to-plant uptake of Cs and Sr in some Egyptian plants grown in Inshas region, Egypt.

J Environ Radioact 2021 May 4;234:106632. Epub 2021 May 4.

Egyptian Nuclear and Radiological Regulatory Authority, Egypt. Electronic address:

The transfer of Cs and Sr from soil to different plants has been studied in two successive pot field-grown experiments. Five plant species (Oryza Sativa, Tritichum Vulgares, Vicia Faba, Sesamum Orientale and Trifolium Alexandrinum) were grown in pots with sandy clay loam soil contaminated with varying amounts of Cs or Sr. The absorption of the radioisotopes was measured in different parts of the plants and their activity concentration was significantly increased with increasing radioactivity of both radioisotopes in the soil. Read More

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Nutritional value of commercial and traditional lettuce (Lactuca sativa L.) and wild relatives: Vitamin C and anthocyanin content.

Food Chem 2021 Apr 20;359:129864. Epub 2021 Apr 20.

Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. Montañana 930, 50059 Zaragoza, Spain; Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), Zaragoza, Spain. Electronic address:

Lettuce is the most consumed leafy vegetable though the most popular varieties have a low nutritional value. Our objective was to accurately quantify vitamin C and anthocyanins in wild relatives, and commercial and traditional varieties. Wild species and traditional varieties contained more total ascorbic acid (TAA) than commercial varieties (21% and 8%, respectively). Read More

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Gamma sterilization of collagen/hydroxyapatite composites: Validation and radiation effects.

Appl Radiat Isot 2021 May 1;174:109758. Epub 2021 May 1.

Universidade Federal de Minas Gerais, Escola de Engenharia, Departamento de Engenharia Metalúrgica e de Materiais, Bloco 2, Sala 2233, Avenida Presidente Antônio Carlos 6627, Pampulha, Belo Horizonte, Minas Gerais, CEP, 31270-901, Brazil. Electronic address:

In this work, gamma sterilization was validated, and the impact of this sterilization process on collagen/hydroxyapatite (Col/HAp) composites was investigated. It has been already recognized that the improper sterilization of healthcare products may lead to infection and mortality/morbidity issues in patients. Gamma sterilization has emerged as a promising sterilization method because it shows advantages such as low cost, a small increase in temperature of irradiated materials, and no production of toxic residues. Read More

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Validating pore size estimates in a complex microfiber environment on a human MRI system.

Magn Reson Med 2021 May 7. Epub 2021 May 7.

Cardiff University Brain Research Imaging Centre (CUBRIC), Cardiff University, Cardiff, United Kingdom.

Purpose: Recent advances in diffusion-weighted MRI provide "restricted diffusion signal fraction" and restricting pore size estimates. Materials based on co-electrospun oriented hollow cylinders have been introduced to provide validation for such methods. This study extends this work, exploring accuracy and repeatability using an extended acquisition on a 300 mT/m gradient human MRI scanner, in substrates closely mimicking tissue, that is, non-circular cross-sections, intra-voxel fiber crossing, intra-voxel distributions of pore-sizes, and smaller pore-sizes overall. Read More

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Impacts of effluent from different livestock farm types (pig, cow, and poultry) on surrounding water quality: a comprehensive assessment using individual parameter evaluation method and water quality indices.

Environ Sci Pollut Res Int 2021 May 6. Epub 2021 May 6.

Department of Environmental Management, Faculty of Environment, Vietnam National University of Agriculture, Hanoi, Vietnam.

Water pollution within and nearby different livestock farm types was assessed comprehensively for the first time in Vietnam. The samples of wastewater, ground water, and surface water were collected from 130 pig farms, 80 poultry farms, and 40 cow farms. Water quality was first assessed by individual parameter evaluation method in which measured values of water quality parameters were compared with the permissible limits in the national technical regulations on livestock's effluent (QCVN 62), surface water quality, and ground water quality. Read More

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Molecular Typing of Community-acquired Methicillin-Resistant Isolated from 2- to 6-year old Children by Staphylococcal Protein A and Typing in Isfahan, Iran.

Adv Biomed Res 2021 27;10. Epub 2021 Jan 27.

Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Methicillin-resistant (MRSA) has become a considerable public health concern in the entire world due to the rapid spread of this bacterium in human community; also the epidemiology of MRSA has changed, as the isolation of MRSA strains from healthy and non-healthy patients. Therefore, the objective of this study is to determine the genetic diversity and antibiotic resistance profile of community-acquired (CA)-MRSA nasal carriage in the Iranian samples.

Materials And Methods: A total of 25 CA-MRSA were isolated from the anterior nares of 410 healthy preschool children. Read More

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January 2021

Progesterone promotes immunomodulation and tumor development in the murine mammary gland.

J Immunother Cancer 2021 May;9(5)

Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas, USA

Background: Clinical studies have linked usage of progestins (synthetic progesterone [P4]) to breast cancer risk. However, little is understood regarding the role of native P4, signaling through the progesterone receptor (PR), in breast tumor formation. Recently, we reported a link between PR and immune signaling pathways, showing that P4/PR can repress type I interferon signaling pathways. Read More

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Microbial and chemical pollutants on the manure-crops pathway in the perspective of "One Health" holistic approach.

Sci Total Environ 2021 Apr 29;785:147411. Epub 2021 Apr 29.

The Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 Str., 44-100 Gliwice, Poland; Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland.

This study determined the impact of poultry and bovine manure fertilization on the content of antibiotics, heavy metals (HMs), and the quantitative and qualitative composition of integrase and antibiotic resistance genes in soil, groundwater, and crops cultivated on manure-amended plots. Antibiotic concentration levels were analyzed using the HPLC-MS/MS, heavy metal concentration level were measured by HGAAS and ICP-OES, while the integrase genes and ARGs were quantified using Real-Time PCR (qPCR) method. Manure, soil, and crops samples contained the highest concentration of Zn (10-10 ng g) and Cu (10-10 ng g) of all HMs tested. Read More

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Analysis of Microbiological and Chemical Hazards in Edible Insects Available to Canadian Consumers.

J Food Prot 2021 May 6. Epub 2021 May 6.

Canadian Food Inspection Agency.

Edible insects are a novel food in most countries; their popularity is growing because of their high protein/low fat content, ease of cultivation, and small environmental impact. To our knowledge, this is the first report that addresses both microbiological and chemical hazards in edible insects. Samples were collected from retail stores or purchase through e-commerce. Read More

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Detection of Salmonella genes in stool samples of children aged 5 years and younger in urban and rural areas of Bangladesh.

J Infect Dev Ctries 2021 Apr 30;15(4):506-515. Epub 2021 Apr 30.

Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

Introduction: Typhoid incidence in children is higher in urban areas than in rural areas of Bangladesh. This study examined whether healthy urban children harboured higher levels of Salmonella genes than healthy rural children.

Methodology: Stool samples from 140 children were studied: 70 from rural areas and 70 from urban metropolitan areas. Read More

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Detection of Species, and -Like and -Like Endosymbionts in and from a Shared Field Site in Georgia, United States of America.

Vector Borne Zoonotic Dis 2021 May 5. Epub 2021 May 5.

Rickettsial Zoonoses Branch, United States Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Two abundant species of aggressive ticks commonly feed on humans in Georgia: the Gulf Coast tick () and the Lone Star tick (). is the primary host of , " Rickettsia andeanae," and a -like endosymbiont (AmacFLE), whereas is the primary host for , , , and a -like endosymbiont (AamCLE). Horizontal transmission of from to by co-feeding has been described, and has been found infrequently in ticks. Read More

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Identification and quantification of plasticizers, bisphenol, and environmental toxic mineral elements residues in medicines from Tunisian markets.

Environ Sci Pollut Res Int 2021 May 6. Epub 2021 May 6.

Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, University of Messina, Messina, Italy.

In this study, 18 plasticizer (phthalates, adipates, sebacates, and others) residues in sixteen medicines available in Tunisian pharmaceutical markets are evaluated by MSPD combined with GC-MS. In parallel, in this research, UPLC-MS/MS technique was performed to detect bisphenol A, and cadmium, lead, chromium, cobalt, nickel, mercury, selenium, and arsenic levels were determined by ICP-MS. The maximum daily ingested mass of mineral elements was calculated and compared with permissible daily exposure limits published by USP NF 2013. Read More

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Domain adaptation and self-supervised learning for surgical margin detection.

Int J Comput Assist Radiol Surg 2021 May 6. Epub 2021 May 6.

School of Computing, Queen's University, Ontario, Canada.

Purpose: One in five women who undergo breast conserving surgery will need a second revision surgery due to remaining tumor. The iKnife is a mass spectrometry modality that produces real-time margin information based on the metabolite signatures in surgical smoke. Using this modality and real-time tissue classification, surgeons could remove all cancerous tissue during the initial surgery, improving many facets of patient outcomes. Read More

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MALDI-MS Profiling of Intact Blood Proteins without the Need for Freezing - Dried Serum Spots as Future Clinical Tools for Patient Screening.

Rapid Commun Mass Spectrom 2021 May 5:e9121. Epub 2021 May 5.

Proteome Center Rostock, Medical Faculty and Natural Science Faculty, University of Rostock, Schillingallee 69, 18057, Rostock, Germany.

Rationale: To open new ways for MALDI-MS-based patient screening, blood serum is the most preferred specimen because of its richness in patho-physiological information and due to ease of collection. To overcome deleterious freeze/thaw cycles and to reduce high costs for shipping and storage, we sought to develop a procedure which enables MALDI-MS protein profiling of blood serum proteins without the need for serum freezing.

Methods: Blood sera from patients/donors were divided into portions which after pre-incubation were fast frozen. Read More

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Apolipoprotein B Is Associated With the Microenvironment of Cholangiocarcinoma.

Front Oncol 2021 31;11:654689. Epub 2021 Mar 31.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Cholangiocarcinoma (CCA) is a kind of devastating malignancy, which is correlated with the extremely high mortality. Due to the occult pathogenesis of CCA, most patients are diagnosed in the advanced stage. However, the efficacy of chemotherapy and immunotherapy is limited for these patients. Read More

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PCR Testing of Media Placed in Soiled Bedding as a Method for Mouse Colony Health Surveillance.

J Am Assoc Lab Anim Sci 2021 May 4. Epub 2021 May 4.

Rodent colony health surveillance has traditionally been accomplished by testing sentinel animals that have been exposed to soiled bedding from colony animals. Collecting samples from exhaust plenums on ventilated caging systems, followed by PCR analysis, has emerged as another promising method for health surveillance. However, environmental testing at the rack level is not effective for all ventilated rack designs. Read More

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Whole lung tissue is the preferred sampling method for amplicon-based characterization of murine lung microbiota.

Microbiome 2021 May 5;9(1):99. Epub 2021 May 5.

Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA.

Background: Low-biomass microbiome studies (such as those of the lungs, placenta, and skin) are vulnerable to contamination and sequencing stochasticity, which obscure legitimate microbial signal. While human lung microbiome studies have rigorously identified sampling strategies that reliably capture microbial signal from these low-biomass microbial communities, the optimal sampling strategy for characterizing murine lung microbiota has not been empirically determined. Performing accurate, reliable characterization of murine lung microbiota and distinguishing true microbial signal from noise in these samples will be critical for further mechanistic microbiome studies in mice. Read More

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No association between adult sex steroids and hand preference in humans.

Am J Hum Biol 2021 May 5:e23605. Epub 2021 May 5.

School of Earth and Environmental Sciences, The University of Manchester, Manchester, UK.

Background: There is ongoing debate about the effects of hormones on the lateralization of the developing brain. In humans, there are conflicting theories of how testosterone during development should affect lateralization. Empirical studies linking prenatal and postnatal testosterone levels to hand preference (a proxy for lateralization) are similarly mixed. Read More

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Establishment and verification of prognostic model for gastric cancer based on autophagy-related genes.

Am J Cancer Res 2021 15;11(4):1335-1346. Epub 2021 Apr 15.

Department of Gastroenterology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer Tianjin 300060, P. R. China.

Autophagy played a significant role in the development of cancer. In this study, we explored the value of autophagy-associated genes in gastric cancer. RNA sequencing and clinical information containing 375 gastric cancer and 32 normal tissues were gathered from the TCGA portal. Read More

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Development of a Tellurium Speciation Study Using IC-ICP-MS on Soil Samples Taken from an Area Associated with the Storage, Processing, and Recovery of Electrowaste.

Molecules 2021 Apr 30;26(9). Epub 2021 Apr 30.

Institute of Environmental Engineering Polish Academy of Sciences, M. Sklodowskiej-Curie 34 St., 41-819 Zabrze, Poland.

The optimization and validation of a methodology for determining and extracting inorganic ionic Te(VI) and Te(IV) forms in easily-leached fractions of soil by Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry (IC-ICP-MS) were studied. In this paper, the total concentration of Te, pH, and red-ox potential were determined. Ions were successfully separated in 4 min on a Hamilton PRPX100 column with 0. Read More

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RNAi-Mediated Silencing of the Chitinase 5 Gene for Fall Webworm () Can Inhibit Larval Molting Depending on the Timing of dsRNA Injection.

Insects 2021 Apr 30;12(5). Epub 2021 Apr 30.

Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.

Chitinases, which are crucial enzymes required for chitin degradation and reconstruction, are often selectively considered to be effective molecular targets for pest control due to their critical roles in insect development. Although the chitinase gene has been reported previously, its sequence characteristics, gene function, and feasibility as a potential target for pest management were absent. In the present study, we characterized the chitinase gene and designated it . Read More

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Dead or alive: microbial viability treatment reveals both active and inactive bacterial constituents in the fish gut microbiota.

J Appl Microbiol 2021 May 4. Epub 2021 May 4.

Faculty of Science Engineering and Built Environment, School of Life and Environmental Sciences, Deakin University, Geelong, Vic., Australia.

Aims: This study evaluated the microbial viability of fish gut microbiota in both digesta (faecal) and mucosal samples using a modified propidium monoazide (PMA) protocol, followed by 16S ribosomal RNA (rRNA) gene sequencing.

Methods And Results: Digesta and gut mucosal samples from farmed yellowtail kingfish (Seriola lalandi) were collected and a modified PMA treatment was applied prior to DNA extraction to differentiate both active and nonviable microbial cells in the samples. All samples were then sequenced using a standard 16S rRNA approach. Read More

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Evaluation of fully oxidized β-carotene as a feed ingredient to reduce bacterial infection and somatic cell counts in pasture-fed cows with subclinical mastitis.

Authors:
S McDougall

N Z Vet J 2021 May 4:1-21. Epub 2021 May 4.

Cognosco, Anexa, PO Box 21, Morrinsville, 3300, New Zealand.

Aims: To assess the effect of oral supplementation with fully oxidised β-carotene (OxBC) on bacteriological cure, incidence of clinical mastitis, and somatic cell counts (SCC) in pasture-fed cows with subclinical intramammary infection.

Methods: Cows from four dairy herds were enrolled in early lactation if they had quarter-level SCC >200,000 cells/mL and they had a recognised bacterial intramammary pathogen in one or more quarters. They were randomly assigned to be individually fed from Day 0, for a mean of 40 days, with 0. Read More

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The genomes of ancient date palms germinated from 2,000 y old seeds.

Proc Natl Acad Sci U S A 2021 May;118(19)

Center for Genomics and Systems Biology, New York University, 129188 Abu Dhabi, United Arab Emirates;

Seven date palm seeds ( L.), radiocarbon dated from the fourth century BCE to the second century CE, were recovered from archaeological sites in the Southern Levant and germinated to yield viable plants. We conducted whole-genome sequencing of these germinated ancient samples and used single-nucleotide polymorphism data to examine the genetics of these previously extinct Judean date palms. Read More

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Enhanced reactivity of zero-valent aluminum/O by using Fe-bearing clays in 4-chlorophenol oxidation.

Sci Total Environ 2021 Jun 6;773:145661. Epub 2021 Feb 6.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China. Electronic address:

Zero-valent aluminum (ZVAl) is a promising reductant because of its relatively low redox potential, which can efficiently activate molecular oxygen to generate reactive oxygen species. However, its long-term performance is limited by the intrinsic dense oxide layer and the passivation effect of the accumulative Al-(hydr)oxide on its surface during the reaction. In this study, four clay minerals with different compositions were mixed with ZVAl by ball milling to obtain four composites of ZVAl and clay (ZVAl-Clay), which were used to degrade a high concentration of 4-chlorophenol (4-CP) under ambient conditions. Read More

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Macromineral and trace element concentrations and their seasonal variation in milk from organic and conventional dairy herds.

Food Chem 2021 Apr 20;359:129865. Epub 2021 Apr 20.

Department of Animal Sciences, School of Agriculture, Policy and Development, University of Reading, PO Box 237, Earley Gate, Reading RG6 6EU, United Kingdom. Electronic address:

To study the effects of dairy production system on milk macromineral and trace element concentrations, milk samples were collected monthly in 2019 from 43 conventional and 27 organic farms. Organic milk contained more Ca (1049.5 vs. Read More

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Mobilized Peripheral Blood Stem Cells are Pluripotent and Can be Safely Harvested and Stored for Cartilage Repair.

Arthroscopy 2021 Apr 30. Epub 2021 Apr 30.

Kuala Lumpur Sports Medicine Centre, 47, Jalan Dungun, Bukit Damansara Kuala Lumpur, Malaysia.

Purpose: The primary objective of this study was to reproduce and validate the harvest, processing and storage of peripheral blood stem cells for a subsequent cartilage repair trial, evaluating safety, reliability, and potential to produce viable, sterile stem cells.

Methods: Ten healthy subjects (aged 19-44 years) received three consecutive daily doses of filgrastim followed by an apheresis harvest of mononuclear cells on a fourth day. In a clean room, the apheresis product was prepared for cryopreservation and processed into 4mL aliquots. Read More

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Association between medical male circumcision and HIV risk compensation among heterosexual men: a systematic review and meta-analysis.

Lancet Glob Health 2021 Apr 30. Epub 2021 Apr 30.

School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China; Kirby Institute, University of New South Wales, Sydney, NSW, Australia; School of Public Health, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Background: Medical male circumcision (MMC) reduces HIV infection among heterosexual men. There are concerns MMC might prompt higher-risk sexual behaviours because of lower self-perceived risk of HIV infection. We reviewed the published literature to examine associations between MMC and both condom use and number of sex partners among heterosexual men. Read More

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Formulation of diets for pigs based on a ratio between digestible calcium and digestible phosphorus results in reduced excretion of calcium in urine without affecting retention of calcium and phosphorus compared with formulation based on values for total calcium.

J Anim Sci 2021 May 3. Epub 2021 May 3.

Division of Nutritional Sciences, University of Illinois, Urbana, USA.

An experiment was conducted to test the hypothesis that formulating diets for pigs based on a ratio between standardized total tract digestible (STTD) Ca and STTD P instead of total Ca and STTD P does not decrease Ca retention, but increases P utilization. Forty barrows (59.4 ± 3. Read More

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