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Rare earth elements in uranium ore deposits from Namibia: A nuclear forensics tool.

J Environ Radioact 2021 Jun 8;237:106668. Epub 2021 Jun 8.

Department of Geography and Environmental Management, University of the West of England, UK. Electronic address:

Rare earth elements (REE) concentrations and pattern remains largely unchanged during the process of milling and can thus provide strong evidence of the origin of the material. The aim of this study was to determine the rare earth elements in uranium ore deposits as a nuclear forensics tool. Uranium ore from three mines were collected and analyzed using an inductively coupled plasma mass spectrometry (ICP-MS) to determine the concentrations of rare earth elements. Read More

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Characterization and prediction of viral loads of Hepatitis B serum samples by using surface-enhance Raman spectroscopy (SERS).

Photodiagnosis Photodyn Ther 2021 Jun 8:102386. Epub 2021 Jun 8.

PCR Laboratory, PINUM Hospital, Faisalabad, Pakistan.

Background: Raman spectroscopy is a promising technique to analyze the body fluids for the purpose of non-invasive disease diagnosis.

Objectives: To develop a surface-enhanced Raman spectroscopy (SERS) based method for qualitative and quantitative analysis of hepatitis B viral (HBV) infection from blood serum samples.

Methods: Clinically diagnosed hepatitis B virus (HBV) infected serum samples of patients of different levels of viral loads have been subjected for SERS analysis in comparison with the healthy ones by using silver nanoparticles (Ag NPs) based SERS substrates. Read More

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CtNorm: Real time PCR cycle of threshold (Ct) normalization algorithm.

Authors:
Amin Ramezani

J Microbiol Methods 2021 Jun 8:106267. Epub 2021 Jun 8.

Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran; Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address:

In relative quantification with Real Time PCR (qRT-PCR,), accurate analysis requires equal amplification efficiency for both genes (Gene of interest and reference gene) and equal threshold values for all the samples. In this quantification method the expression level in treated samples will be calculated in comparison to the control group. We conducted the present study to design an algorithm for converting the data obtained from different runs containing identical standard samples into one run with the same amplification efficiency and threshold value. Read More

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The p16/ki-67 assay is a safe, effective and rapid approach to triage women with mild cervical lesions.

PLoS One 2021 11;16(6):e0253045. Epub 2021 Jun 11.

1st Department of Obstetrics and Gynecology, "Alexandra" Hospital, National and Kapodistrian University of Athens, Athens, Greece.

Objective: The aim of this study was to evaluate the diagnostic accuracy and efficiency of p16/ki-67 dual stain in the identification of CIN2+ lesions, in Greek women with ASCUS or LSIL cytology.

Methods: A total of 200 women, 20 to 60 years old, were enrolled in the study. All samples were cytologically evaluated and performed for p16/ki-67 and high-risk HPV (HR-HPV) test. Read More

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Patterns of regulatory behavior in the still-face paradigm at 3 months: A comparison of Brazilian and Portuguese infants.

PLoS One 2021 11;16(6):e0252562. Epub 2021 Jun 11.

Departamento de Fonoaudiologia da Faculdade de Odontologia de Bauru, University of São Paulo, São Paulo, Brazil.

Three infant regulatory behavior patterns have been identified during the Face-to-Face Still-Face paradigm (FFSF) in prior research samples: a Social-Positive Oriented pattern (i.e., infants exhibit predominantly positive social engagement), a Distressed-Inconsolable pattern (i. Read More

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Structure and variation of root-associated bacterial communities of Cyperus rotundus L. in the contaminated soils around Pb/Zn mine sites.

Environ Sci Pollut Res Int 2021 Jun 11. Epub 2021 Jun 11.

National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, 808 Tianyuan Road, Guangzhou, 510650, Guangdong, China.

Soil contamination due to mining activities is a great concern in China. Although the effects of mining pollution resulting in changes of soil characteristics and the microbiome have been documented, studies on the responses of plant root-associated microbial assemblages remain scarce. In this work, we collected bulk soil, rhizosphere soil, and root endosphere samples of Cyperus rotundus L (Cyp) plants from two Pb/Zn mines, of which, one was abandoned (SL) and the other was active (GD), to investigate the bacterial community responses across different site contamination levels and Cyp plant compartments. Read More

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Comparison of Symptoms and RNA Levels in Children and Adults With SARS-CoV-2 Infection in the Community Setting.

JAMA Pediatr 2021 Jun 11. Epub 2021 Jun 11.

Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle.

Importance: The association between COVID-19 symptoms and SARS-CoV-2 viral levels in children living in the community is not well understood.

Objective: To characterize symptoms of pediatric COVID-19 in the community and analyze the association between symptoms and SARS-CoV-2 RNA levels, as approximated by cycle threshold (Ct) values, in children and adults.

Design, Setting, And Participants: This cross-sectional study used a respiratory virus surveillance platform in persons of all ages to detect community COVID-19 cases from March 23 to November 9, 2020. Read More

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Quality of Life and Participation of Children With Visual Impairment: Comparison With Population Reference Scores.

Invest Ophthalmol Vis Sci 2021 Jun;62(7):14

Department of Ophthalmology, Amsterdam UMC, Vrije Universiteit Amsterdam, the Amsterdam Public Health Research Institute, MB Amsterdam, The Netherlands.

Purpose: The purpose of this study was to investigate quality of life and participation in children aged 3 to 17 years with visual impairment (VI) compared to reference groups and between subgroups with increasing severity levels of VI.

Methods: Parents of children aged 3 to 17 years (n = 500) and children aged 13 to 17 years (n = 75) completed the Child and Adolescent Scale of Participation (CASP). Children aged 7 to 17 years (n = 263) and their parents (n = 255) completed the KIDSCREEN-27 questionnaire to assess quality of life. Read More

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An emerging role of Prevotella histicola on estrogen deficiency-induced bone loss through the gut microbiota-bone axis in postmenopausal women and in ovariectomized mice.

Am J Clin Nutr 2021 Jun 10. Epub 2021 Jun 10.

Department of Orthopaedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background: The gut microbiota (GM)-bone axis has emerged as a crucial mediator of bone homeostasis. Estrogen deficiency-induced bone loss is closely associated with an altered GM. However, the underlying mechanisms remain unclear. Read More

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Etiology-Discriminative Multimodal Imaging of Left Ventricular Hypertrophy and Synchrotron-Based Assessment of Microstructural Tissue Remodeling.

Front Cardiovasc Med 2021 25;8:670734. Epub 2021 May 25.

Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.

Distinguishing the etiology of left ventricular hypertrophy (LVH) is clinically relevant due to patient outcomes and management. Easily obtained, echocardiography-based myocardial deformation patterns may improve standard non-invasive phenotyping, however, the relationship between deformation phenotypes and etiology-related, microstructural cardiac remodeling has not been reported. Synchrotron radiation-based X-ray phase-contrast imaging (X-PCI) can provide high resolution, three-dimensional (3D) information on myocardial microstructure. Read More

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Differential methylation of G-protein coupled receptor signaling genes in gastrointestinal neuroendocrine tumors.

Sci Rep 2021 Jun 10;11(1):12303. Epub 2021 Jun 10.

Huntsman Cancer Institute, University of Utah, 2000 Circle of Hope, Salt Lake City, UT, 84112-5550, USA.

Neuroendocrine tumors (NETs) of the small intestine undergo large chromosomal and methylation changes. The objective of this study was to identify methylation differences in NETs and consider how the differentially methylated genes may impact patient survival. Genome-wide methylation and chromosomal copy number variation (CNV) of NETs from the small intestine and appendix were measured. Read More

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GPC3 affects the prognosis of lung adenocarcinoma and lung squamous cell carcinoma.

BMC Pulm Med 2021 Jun 10;21(1):199. Epub 2021 Jun 10.

Molecular Oncology Department of Cancer Research Institution, The First Hospital of China Medical University, Nanjingbei Street, Heping District, Shenyang, 110001, Liaoning Province, China.

Background: Glypican 3 (GPC3) is a heparin sulphate proteoglycan whose expression is associated with several malignancies. However, its expression in non-small-cell lung carcinoma (NSCLC) is limited and ambiguous. This study aimed to comprehensively evaluate the expression of GPC3 in NSCLC and develop a risk-score model for predicting the prognosis of NSCLC. Read More

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Internal validation and comparison of the prognostic performance of models based on six emergency scoring systems to predict in-hospital mortality in the emergency department.

BMC Emerg Med 2021 Jun 10;21(1):68. Epub 2021 Jun 10.

Department of Emergency Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Background: Medical scoring systems are potentially useful to make optimal use of available resources. A variety of models have been developed for illness measurement and stratification of patients in Emergency Departments (EDs). This study was aimed to compare the predictive performance of the following six scoring systems: Simple Clinical Score (SCS), Worthing physiological Score (WPS), Rapid Acute Physiology Score (RAPS), Rapid Emergency Medicine Score (REMS), Modified Early Warning Score (MEWS), and Routine Laboratory Data (RLD) to predict in-hospital mortality. Read More

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Weaning at 30, 75 and 180 days: Comparison between immune responses of beef calves.

Res Vet Sci 2021 Jun 2;138:53-61. Epub 2021 Jun 2.

Department of Science Animal, Universidade Federal do Rio Grande do Sul, Ave. Bento Gonçalves, 91540000 Porto Alegre, Brazil.

The interruption of the maternal-filial bond in young calves can alter the physiological responses due to increased stress at this moment. Therefore, we aimed to analyze the association between age at weaning and the immune responses of 64 beef calves weaned abruptly. The animals were distributed in three weaning (W) treatments: hyper-early (W30), early (W75) and conventional (W180). Read More

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Monitoring HOTTIP levels on extracellular vesicles for predicting recurrence in surgical non-small cell lung cancer patients.

Transl Oncol 2021 Jun 7;14(8):101144. Epub 2021 Jun 7.

Molecular Oncology and Embryology Laboratory, Human Anatomy Unit, Faculty of Medicine and Health Sciences, University of Barcelona, IDIBAPS, Casanova 143, 08036 Barcelona, Spain; Thoracic Oncology Unit, Hospital Clinic, Barcelona. Electronic address:

In resected non-small cell lung cancer (NSCLC), postsurgical recurrence is the major factor affecting long-term survival. The identification of biomarkers in extracellular vesicles (EV) obtained from serial blood samples after surgery could enhance early detection of relapse and improve NSCLC outcome. Since EV cargo contains long non-coding RNAs (lncRNAs), we aimed to analyze whether the oncogenic lncRNA HOTTIP, which higher expression in tumor tissue was related to worse outcome in NSCLC, could be detected in EV from NSCLC patients and serve as recurrence biomarker. Read More

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The Ability of Dental Practitioners to Light-Cure Simulated Restorations.

Oper Dent 2021 Jun 10. Epub 2021 Jun 10.

Clinical Relevance: Using a patient simulator, dental professionals were tested to determine their ability to light-polymerize simulated restorations in their dental practice. After receiving specific instructions and training using the simulator, their ability to deliver sufficient light to polymerize restorations was significantly and substantially improved.

Summary: Objectives: To determine the ability of dental professionals to deliver a radiant exposure of at least six J/cm2 in 10 seconds to simulated restorations. Read More

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Low level SARS-CoV-2 RNA detected in plasma samples from a cohort of Nigerians: Implications for blood transfusion.

PLoS One 2021 10;16(6):e0252611. Epub 2021 Jun 10.

Centre for Human Virology and Genomics, Microbiology Department, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria.

The present global pandemic triggered by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has lingered for over a year in its devastating effects. Diagnosis of coronavirus disease 2019 (COVID-19) is currently established with a polymerase chain reaction (PCR) test by means of oropharyngeal-, nasopharyngeal-, anal-swabs, sputum and blood plasma. However, oral and nasal swabs are more commonly used. Read More

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A comparison expression analysis of CXCR4, CXCL9 and Caspase-9 in dermal vascular endothelial cells between keloids and normal skin on chemotaxis and apoptosis.

J Plast Surg Hand Surg 2021 Jun 10:1-10. Epub 2021 Jun 10.

The Sixteenth Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Beijing, China.

This present study was designed to explore key biological characteristics and biomarkers associated with dermal vascular endothelial cells of keloids. GSE121618 dataset was downloaded in the Gene Expression Omnibus (GEO) Database, including the KECs group and NVECs group. Through GEO2R, we have screened the differentially expressed genes (DEGs) and performed gene ontology (GO), Gene Set Enrichment Analysis (GSEA), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Read More

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A Universal and High-Throughput Proteomics Sample Preparation Platform.

Anal Chem 2021 Jun 10. Epub 2021 Jun 10.

National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Rockville, Maryland 20850, United States.

Major advances have been made to improve the sensitivity of mass analyzers, spectral quality, and speed of data processing enabling more comprehensive proteome discovery and quantitation. While focus has recently begun shifting toward robust proteomics sample preparation efforts, a high-throughput proteomics sample preparation is still lacking. We report the development of a highly automated universal 384-well plate sample preparation platform with high reproducibility and adaptability for extraction of proteins from cells within a culture plate. Read More

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A comparison of Treponema pallidum subspecies pallidum molecular typing systems: multilocus sequence typing (MLST) vs enhanced CDC Typing (ECDCT).

Sex Transm Dis 2021 Mar 16. Epub 2021 Mar 16.

Department of Neurologya, University of Washington, Seattle, WA.

Background: At least three syphilis typing systems are proposed. Recent work suggests that multilocus sequence typing (MLST) may be superior to enhanced CDC typing (ECDCT) by yielding a higher discriminatory power. The goal of this study was to compare the two systems and identify associations between neurosyphilis and strain types. Read More

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Pollution characteristics and human health risk assessments of toxic metals and particle pollutants via soil and air using geoinformation in urbanized city of Pakistan.

Environ Sci Pollut Res Int 2021 Jun 10. Epub 2021 Jun 10.

Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Haikou, 570228, Hainan, China.

Toxic metals and particle pollutants in urbanized cities have significantly increased over the past few decades mainly due to rapid urbanization and unplanned infrastructure. This research aimed at estimating the concentration of toxic metals and particle pollutants and the associated risks to public health across different land-use settings including commercial area (CA), urban area (UA), residential area (RA), and industrial area (IA). A total of 47 samples for both soil and air were collected from different land-use settings of Faisalabad city in Pakistan. Read More

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Adaptive Behavior Profiles of Intellectually Gifted Children with Autism Spectrum Disorder.

J Dev Behav Pediatr 2021 Jun-Jul 01;42(5):374-379

Department of Psychiatry, University of Colorado Anschutz Medical Campus, Aurora, CO.

Objective: The purpose of this study is to identify whether the well-described pattern of declining adaptive functioning across age among children with autism spectrum disorder (ASD) also exists among intellectually gifted children with ASD because their cognitive abilities might serve as a protective factor.

Methods: Data from the Simons Simplex Collection were used to identify 51 participants with full-scale intelligence (IQ) scores of 130 or above with this group labeled as the intellectually gifted range (IGR). Two comparison samples of children with IQs in the intellectual disability range (IDR; < 70 Standard Score [SS]) and average range (AR; 85-115 SS) were created based on matching of age (±2 years), maternal education level, and sex. Read More

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Comparison of Percutaneous Transforaminal Endoscopic Discectomy and Microscope-Assisted Tubular Discectomy for Lumbar Disc Herniation.

Orthop Surg 2021 Jun 10. Epub 2021 Jun 10.

Department of Spinal Surgery, Qingdao Chest Hospital, Qingdao, China.

Objective: The aim of the present study was to compare the clinical outcomes and quality of life following percutaneous transforaminal endoscopic discectomy (PTED) and microscope-assisted tubular discectomy (MTD) for lumbar disc herniation (LDH).

Methods: This study had a retrospective design. From June 2017 to June 2018, the clinical data of 120 patients with LDH treated with PTED (60 cases, PTED group) and MTD (60 cases, MTD group) were analyzed and followed up for at least 20 months. Read More

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Comparison of keratometry data using handheld and table-mounted instruments in healthy adults.

Int Ophthalmol 2021 Jun 9. Epub 2021 Jun 9.

Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.

Purpose: To compare keratometry data between the handheld Retinomax K-plus 3 and the table-mounted IOLMaster 700.

Methods: Healthy adult volunteers were prospectively recruited to the study. All participants underwent 3 consecutive keratometry measurements using the Retinomax K-plus 3 and a single biometry assessment using the IOLMaster 700. Read More

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A Cross-National Comparison of Myalgic Encephalomyelitis and Chronic Fatigue Syndrome at Tertiary Care Settings from the US and Spain.

Am J Soc Sci Humanit 2020 19;5(1):104-115. Epub 2019 Dec 19.

Vall d'Hebron University Hospital, CFS/ME Unit, Universitat Autònoma de Barcelona, Barcelona, Spain.

Cross-national comparative studies are useful for describing the unique characteristics of complex illnesses, and can reveal culture-specific traits of disease frequency/severity and healthcare. Though myalgic encephalomyelitis (ME) and chronic fatigue syndrome (CFS) are debilitating conditions found all over the world, few studies have examined their characteristics across different countries. The purpose of this study was to compare the levels of functional impairment and symptomatology in patients with ME and CFS at tertiary referral hospitals in the US and Spain. Read More

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December 2019

Molecular Identification and Genetic Characterization of Early-Stage Multiple Primary Lung Cancer by Large-Panel Next-Generation Sequencing Analysis.

Front Oncol 2021 24;11:653988. Epub 2021 May 24.

Department of Thoracic Surgery, Beijing Haidian Hospital, Beijing, China.

Objective: The incidence of early stage multiple primary lung cancer (MPLC) has been increasing in recent years, while the ideal strategy for its diagnosis and treatment remains controversial. The present study conducted genomic analysis to identify a new molecular classification method for accurately predicting the diagnosis and therapy for patients with early stage MPLC.

Methods: A total of 240 tissue samples from 203 patients with multiple-non-small-cell lung cancers (NSCLCs) (n = 30), early stage single-NSCLC (Group A, n = 94), and advanced-stage NSCLC (Group B, n = 79) were subjected to targeted multigene panel sequencing. Read More

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Comparison of SARS-CoV-2 Antibody Responses and Seroconversion in COVID-19 Patients Using Twelve Commercial Immunoassays.

Ann Lab Med 2021 Nov;41(6):577-587

Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody assays have high clinical utility in managing the pandemic. We compared antibody responses and seroconversion of coronavirus disease 2019 (COVID-19) patients using different immunoassays.

Methods: We evaluated 12 commercial immunoassays, including three automated chemiluminescent immunoassays (Abbott, Roche, and Siemens), three enzyme immunoassays (Bio-Rad, Euroimmun, and Vircell), five lateral flow immunoassays (Boditech Med, SD biosensor, PCL, Sugentech, and Rapigen), and one surrogate neutralizing antibody assay (GenScript) in sequential samples from 49 COVID-19 patients and 10 seroconversion panels. Read More

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November 2021

Analytical Evaluation of Lumipulse Anti-Mullerian Hormone Assay.

Clin Lab 2021 Jun;67(6)

Background: The measurement of serum Anti-Mullerian hormone (AMH) is used in daily practice to estimate the ovarian reserve in women.

Methods: The aim of this study was to evaluate the new Lumipulse AMH® immunoassay (Fujirebio) with regard to the reliability with two preexisting assays: Elecsys AMH Plus® (cobas®, Roche) and Vidas AMH® (bioMérieux). Precision of Lumipulse AMH was evaluated on the Lumipulse G600 II using the manufacturer's quality controls. Read More

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[Protective effect of baicalein on high fat-induced hepatocytes oxidative damage].

Zhonghua Gan Zang Bing Za Zhi 2021 May;29(5):462-467

School of Nursing, Hubei University of Chinese Medicine, Wuhan 430000, China.

To investigate the effect of baicalein in improving non-alcoholic fatty liver disease caused by high fat-induced oxidative damage in mice. Male C57BL/6J mice weighing 18-20 g were randomly divided into 4 groups: normal control group (C, 10% fat for energy), high-fat group (H, 60% fat for energy), high-fat + scutellaria baicalein group (H+B, baicalein: 400 mg·kg(-1)·bw(-1)), and baicalein control group (B, baicalein: 400 mg·kg(-1)·bw(-1)). After 12 weeks, mice were sacrificed, and the tissue samples were collected. Read More

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Enantiomeric methadone quantitation on real post-mortem dried matrix spots samples: Comparison of liquid chromatography and supercritical fluid chromatography coupled to mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 May 14;1177:122755. Epub 2021 May 14.

Alpine Foundation for Life Sciences (FASV), 6718 Olivone, Switzerland.

This study describes two bioanalytical methods for the quantitation of the two methadone enantiomers in dried matrix spots using high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) and high performance supercritical chromatography tandem mass spectrometry (HPSFC-MS/MS). Dried matrix spots were obtained by spotting 10 µL of each sample fluid on a Whatman paper. Methadone and its main metabolite, EDDP, were extracted with 100 µL methanol and subsequently injected into the LC-MS/MS and SFC-MS/MS systems. Read More

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