288,060 results match your criteria samples animal

Evaluation of two Fasciola hepatica faecal egg counting protocols in sheep and cattle.

Vet Parasitol 2021 Apr 27;294:109435. Epub 2021 Apr 27.

Institute of Biological, Environmental & Rural Sciences, Aberystwyth University, Penglais Campus, Aberystwyth, Ceredigion, SY23 3FG, United Kingdom.

Fascioliasis causes significant economic losses and is a constant challenge to livestock farmers globally. Fluke faecal egg counts (flukeFECs) are a simple, non-invasive method used to detect the presence of patent liver fluke infection. Many flukeFEC techniques exist but they vary in complexity, precision and accuracy. Read More

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Upregulated-gene expression of pro-inflammatory cytokines, oxidative stress and apoptotic markers through inflammatory, oxidative and apoptosis mediated signaling pathways in bovine pneumonia.

Microb Pathog 2021 May 1:104935. Epub 2021 May 1.

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. Electronic address:

Pneumonia is the acute inflammation of lung tissue and is multi-factorial in etiology. Staphylococcus aureus (S. aureus) is a harmful pathogen present as a normal flora of skin and nares of dairy cattle. Read More

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Identification of a variant in NDP associated with X-linked retinal dysplasia in the English cocker spaniel dog.

PLoS One 2021 4;16(5):e0251071. Epub 2021 May 4.

Department of Canine Genetics, Kennel Club Genetics Centre, Animal Health Trust, Kentford, Newmarket, United Kingdom.

Purpose: Three related male English Cocker Spaniels (ECS) were reported to be congenitally blind. Examination of one of these revealed complete retinal detachment. A presumptive diagnosis of retinal dysplasia (RD) was provided and pedigree analysis was suggestive of an X-linked mode of inheritance. Read More

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The Horse as a Therapist: Effects of an Equine Program Without "Therapy" on the Attention and Behavior of Youth Disengaged from Traditional School.

J Altern Complement Med 2021 May 4. Epub 2021 May 4.

The Hopkins Centre, Menzies Institute Queensland, School of Health Sciences and Social Work, Griffith University, Nathan Campus, Australia.

Equine-assisted therapy may promote positive behavior change in young people "at risk." However, it is not always clear what therapeutic content is involved and if a trained therapist is included. The therapeutic effects of the key part of the "therapy," the horse, are not understood. Read More

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Rheumatoid factor and falsely elevated results in commercial immunoassays: data from an early arthritis cohort.

Rheumatol Int 2021 May 4. Epub 2021 May 4.

Department of Medical Biochemistry, Oslo University Hospital-Radiumhospitalet, Box 4953 Nydalen, 0424, Oslo, Norway.

The aim of the study was to  assess RF cross-reactivity to animal antibodies used in immunoassays, and to test if selected commercial immunoassays are vulnerable to interference from RF, causing false test results. Our study included samples from patients with RF-positive rheumatoid arthritis (RA) and controls (patients with RF-negative RA and psoriatic arthritis), included in an early arthritis-cohort. Reactivity to mouse IgG1, mouse IgG2a, rabbit IgG, bovine IgG, sheep/goat IgG and human IgG was analysed using in-house interference assays. Read More

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Proliferation of Peripheral Blood Mononuclear Cells From Healthy Piglets After Mitogen Stimulation As Indicators of Disease Resilience.

J Anim Sci 2021 May 4. Epub 2021 May 4.

Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, SK, Canada.

Disease resilience refers to productivity of an animal under disease. Given the high biosecurity of pig nucleus herds, traits that can be measured on healthy pigs and that are genetically correlated with disease resilience, i.e. Read More

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A novel loop-mediated isothermal amplification-lateral-flow-dipstick (LAMP-LFD) device for rapid detection of Toxoplasma gondii in the blood of stray cats and dogs.

Parasite 2021 3;28:41. Epub 2021 May 3.

Department of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.

Toxoplasma gondii is an obligate intracellular protozoan parasite that causes toxoplasmosis and threatens warm-blooded animal and human health worldwide. Simple and applicable diagnostic methods are urgently needed to guide development of effective approaches for prevention of toxoplasmosis. Most molecular diagnostic tools for T. Read More

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Changes of fibrinolytic system in thrombolytic resuscitation of pulmonary thromboembolism-induced cardiac arrest model.

Int Wound J 2021 May 4. Epub 2021 May 4.

Department of Emergency, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

The objective of this study is to explore the changes in the coagulation and fibrinolysis system in an animal model with pulmonary embolism after cardiopulmonary bypass and to provide a theoretical basis for clinical practice. An animal model of cardiac arrest due to pulmonary embolism was established for venous thrombus (10-15 mL) in the left external jugular vein of 21 pigs. Computed tomography (CT) pulmonary arteriography was performed after the recovery of the underlying state, cardiac arrest state and spontaneous circulation, and then thrombolysis and cardiopulmonary resuscitation (recombinant tissue plasminogen activator [t-PA] 50 mg) were performed immediately. Read More

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Cytologic, histopathologic, and clinical features of granulomatous colitis in a French Bulldog.

Vet Clin Pathol 2021 May 3. Epub 2021 May 3.

Department of Comparative, Diagnostic, & Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.

A young French Bulldog was presented with clinical signs of chronic gastrointestinal disease, unresponsive to medical therapies. Parasite screenings and abdominal ultrasound failed to identify the etiology of the clinical signs. Cytologic evaluation of a rectal scraping sample diagnosed presumptive granulomatous colitis (GC) based on the presence of numerous macrophages with characteristic abundant, pink, granular cytoplasm, which showed an intense pink color when stained with periodic acid-Schiff. Read More

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Mycoplasma hyopneumoniae in free-living wild boars in Paraná, Brazil.

Braz J Microbiol 2021 May 3. Epub 2021 May 3.

Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.

This is the first study conducted in Paraná, Brazil, to investigate Mycoplasma hyopneumoniae (Mhyo) infection in free-living wild boars. Eighty-eight wild boars were managed by authorized controllers between 2017 and 2019 in the state of Paraná in southern Brazil. Management georeferencing, sex, and weight were recorded for each animal. Read More

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The total accumulation of heavy metals in body in connection with the dairy productivity of cows.

Environ Sci Pollut Res Int 2021 May 3. Epub 2021 May 3.

Laboratory of Molecular Genetic Research and Metallomics in Cattle Breeding, Federal Research Centre of Biological Systems and Agrotechnologies of Russian Academy of Sciences, Orenburg, Russia.

Monitoring of health status and metabolism of dairy cows is essential for modern milk production. At the current level of productivity of dairy cows, in addition to essential elements, increasing attention must be paid to controlling the exchange of toxic elements, a significant number of which belong to the category of heavy metals. In this regard, the objective of our research was to study the relationship of pools of heavy metals in the body of dairy cows, evaluated according to hair composition and elemental composition of blood serum and milk productivity of animals. Read More

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Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes.

Nat Methods 2021 May 3. Epub 2021 May 3.

Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Competitive coevolution between microbes and viruses has led to the diversification of CRISPR-Cas defense systems against infectious agents. By analyzing metagenomic terabase datasets, we identified two compact families (775 to 803 amino acids (aa)) of CRISPR-Cas ribonucleases from hypersaline samples, named Cas13X and Cas13Y. We engineered Cas13X. Read More

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A global carbon and nitrogen isotope perspective on modern and ancient human diet.

Proc Natl Acad Sci U S A 2021 May;118(19)

Australian Research Council Centre of Excellence for Australian Biodiversity and Heritage, James Cook University, Cairns, QLD 4870, Australia.

Stable carbon and nitrogen isotope analyses are widely used to infer diet and mobility in ancient and modern human populations, potentially providing a means to situate humans in global food webs. We collated 13,666 globally distributed analyses of ancient and modern human collagen and keratin samples. We converted all data to a common "Modern Diet Equivalent" reference frame to enable direct comparison among modern human diets, human diets prior to the advent of industrial agriculture, and the natural environment. Read More

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Effect of a single intra-articular high molecular weight hyaluronan in a naturally occurring canine osteoarthritis model: a randomized controlled trial.

J Orthop Surg Res 2021 May 3;16(1):290. Epub 2021 May 3.

Faculty of Veterinary Medicine, University of Lisbon (FMV/ULisboa), Lisbon, Portugal.

Background: Osteoarthritis (OA) is a complex joint disease and chronic pain source, affecting a patient's quality of life and posing a financial burden. As the dog is considered a nearly ideal species for translation research of human OA and the most used model for research, exploring spontaneous dog OA under the One Health/One Medicine concept can improve both humans and dogs' health and well-being.

Methods: In a clinical treatment experiment, forty (N=40) joints were selected and randomly assigned to a control group (CG), which received 0. Read More

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Wild boar in the city: Phenotypic responses to urbanisation.

Sci Total Environ 2021 Jun 4;773:145593. Epub 2021 Feb 4.

Wildlife Ecology & Health group and Servei d'Ecopatologia de Fauna Salvatge, Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Travessera dels Turons s/n, 08193, Bellaterra, Barcelona, Spain. Electronic address:

Urbanisation is a global human-induced environmental change and one of the most important threats to biodiversity. To survive in human-modified environments, wildlife must adjust to the challenging selection pressures of urban areas through behaviour, morphology, physiology and/or genetic changes. Here we explore the effect of urbanisation in a large, highly adaptable and generalist urban adapter species, the wild boar (Sus scrofa, Linnaeus 1758). Read More

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Intravenous infusion of glucose improved farrowing performance of hyperprolific crossbred sows.

J Anim Sci 2021 May 3. Epub 2021 May 3.

Department of Animal Science, Aarhus University, Foulum, Tjele, Denmark.

The sow at parturition is challenged with respect to energy status due to increases in energetic expenses associated with 1) nest building 2) uterine contractions and 3) colostrum production. A previous study indicated that sows were depleted of glucogenic energy around farrowing. The aim was to investigate whether intravenous infusion of glucose from observed nest building behavior to 24 h postpartum affected the farrowing kinetics and colostrum production in sows. Read More

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Analysis of synthetic antioxidants in salmon silage using QuEChERS extraction method followed by determination by LC-MS/MS; a single-laboratory validation study.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Apr 16;1174:122715. Epub 2021 Apr 16.

ScanBio Marine Group AS, Brattørkaia 17b, NO-7010 Trondheim, Norway.

A new method was developed and in-house validated to determined ethoxyquin (EQ), butylated hydroxyanisole (BHA) and propyl gallate (PG) in fish silage and fish oil used for production of animal feed. Sample pre-treatment comprises use of QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction and gives extracts for liquid chromatography-triple quadrupole mass spectrometry analysis without matrix interferences. The results allow quantification of the antioxidants in all matrices by using deuterated internal standards and calibration curves made in solvent, as well as establishing low detection limits (0. Read More

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Macromineral and trace element concentrations and their seasonal variation in milk from organic and conventional dairy herds.

Food Chem 2021 Apr 20;359:129865. Epub 2021 Apr 20.

Department of Animal Sciences, School of Agriculture, Policy and Development, University of Reading, PO Box 237, Earley Gate, Reading RG6 6EU, United Kingdom. Electronic address:

To study the effects of dairy production system on milk macromineral and trace element concentrations, milk samples were collected monthly in 2019 from 43 conventional and 27 organic farms. Organic milk contained more Ca (1049.5 vs. Read More

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Stable isotopes verify geographical origin of Tibetan chicken.

Food Chem 2021 Apr 19;358:129893. Epub 2021 Apr 19.

Institute of Quality Standard & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:

Due to the particularity of origin area, the yield and price of Tibetan chicken are different from those in the plain areas, which leads to chickens from the plains being disguised as Tibetan chickens. In present study, Tibetan chicken and chicken from other four provinces in plain areas were distinguished by stable isotopes and chemometric methods. The cross-validation rate was 97. Read More

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Impact of feeding modified soy protein concentrate in the starter phase on growth performance and gastrointestinal responses in broiler chickens through to day 42 of age.

Poult Sci 2021 Mar 21;100(6):101147. Epub 2021 Mar 21.

Research and Development, TripleA A/S, Hornsyld, Denmark.

Growth performance and physiological responses of feeding modified soy protein concentrate (MSPC, 72% CP) in the starter phase were investigated. A total of 1,216 d old male Ross x Ross 708 broiler chicks were placed in 32 floor pens based on BW, fed one of 4 (n = 8) corn-soybean meal-based diets formulated with 0, 7.7, 10. Read More

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Fluorescence image-guided resection of intracranial meningioma: an experimental in vivo study on nude mice.

Ann Anat 2021 Apr 30:151752. Epub 2021 Apr 30.

Klinik für Neurochirurgie, Universitätsklinikum des Saarlandes, Homburg, Germany.

Introduction: The use of photodynamic agents in malignant cranial tumor surgery is quite common. For example five-aminolevulinic acid (5-ALA)-induced porphyrins in malignant gliomas are potent photosensitizers. Until today there is no comparable selective fluorescent substance available for meningiomas. Read More

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Low Risk of Transmission of Pathogenic Bacteria between Children and the Assistance Dog during Animal Assisted Therapy if Strict Rules are Followed.

J Hosp Infect 2021 Apr 30. Epub 2021 Apr 30.

Department of Medical Sciences/Section of Clinical Microbiology, Uppsala University, Uppsala, Sweden.

This study explored the bacterial transmission between patients and dogs during dog-assisted therapy (DAT). Twenty children (55% girls) with a median age of 7 years (range 3-17 years) were included. Two dogs assisted and the conditions were more restricted hygienically with dog 2. Read More

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Formulation of diets for pigs based on a ratio between digestible calcium and digestible phosphorus results in reduced excretion of calcium in urine without affecting retention of calcium and phosphorus compared with formulation based on values for total calcium.

J Anim Sci 2021 May 3. Epub 2021 May 3.

Division of Nutritional Sciences, University of Illinois, Urbana, USA.

An experiment was conducted to test the hypothesis that formulating diets for pigs based on a ratio between standardized total tract digestible (STTD) Ca and STTD P instead of total Ca and STTD P does not decrease Ca retention, but increases P utilization. Forty barrows (59.4 ± 3. Read More

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Description and comparison of the skin and ear canal microbiota of non-allergic and allergic German shepherd dogs using next generation sequencing.

PLoS One 2021 3;16(5):e0250695. Epub 2021 May 3.

Department of Dermatology, Small Animal Clinic-Internal Medicine, Justus Liebig University, Giessen, Germany.

Atopic dermatitis is one of the most common skin diseases in dogs. Pathogenesis is complex and incompletely understood. Skin colonizing bacteria likely play an important role in the severity of this disease. Read More

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Prevalence of clinical malaria and household characteristics of patients in tribal districts of Pakistan.

PLoS Negl Trop Dis 2021 May 3;15(5):e0009371. Epub 2021 May 3.

Department of Bioscience and Biotechnology, The University of Suwon, Hwaseong City, Gyeonggi-do, Republic of Korea.

Background: Malaria, disproportionately affects poor people more than any other disease of public health concern in developing countries. In resource-constrained environments, monitoring the occurrence of malaria is essential for the success of national malaria control programs. Militancy and military conflicts have been a major challenge in monitoring the incidence and controlling malaria and other emerging infectious diseases. Read More

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Long-term effects of liquid swine manure land surface application in an apple orchard field on soil bacterial community and heavy metal contents in apple (Malus pumila Mill.).

Environ Sci Pollut Res Int 2021 May 3. Epub 2021 May 3.

Department of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, People's Republic of China.

This study investigated the impact of liquid swine manure (LSM) land surface application in an apple orchard on soil health and copper (Cu) and zinc (Zn) in soil and apple. Three apple plots were selected, among which two for LSM application for 5 (AY5) and 11 (AY11) years with different application rates, a long-term inorganic fertilizer application plot as the control treatment (AY0). The soil and apple samples were collected for analysis of soil physicochemical properties, bacterial diversity and abundance, and the contents of Cu and Zn in soil and apple. Read More

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Serological evidence of bovine viral diarrhea virus and peste des petits ruminants virus infection in alpacas (Vicugna pacos) in Shanxi Province, northern China.

Trop Anim Health Prod 2021 Apr 30;53(2):299. Epub 2021 Apr 30.

College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, People's Republic of China.

Bovine viral diarrhea virus (BVDV) and peste des petits ruminants virus (PPRV) are two important pathogens associated with a variety of disease syndromes that result in substantial financial losses in animal husbandry. This study was performed to assess the seroprevalence of antibodies to BVDV and PPRV among alpacas raised in Shanxi Province of northern China. Serum samples were obtained from 246 alpacas in Taiyuan (n=182), Xinzhou (n=31), and Jinzhong cities (n=33) of Shanxi Province, and serological testing was carried out using the competitive enzyme-linked immunosorbent assay. Read More

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Endoparasite prevalence and infection risk factors among cats in an animal shelter in Estonia.

Folia Parasitol (Praha) 2021 Apr 20;68. Epub 2021 Apr 20.

Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Estonia.

Cats are important hosts for different zoonotic parasites that can be hazardous to human health. To date, few studies have attempted to identify the factors affecting parasitic infections in shelter animals. This study aims to analyse the presence of endoparasites in shelter cats in Tartu, Estonia, and identify factors affecting endoparasite prevalence and intensity. Read More

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Dihydroartemisinin ameliorates balloon injury-induced neointimal formation through suppressing autophagy in vascular smooth muscle cells.

Biol Chem 2021 Mar 9;402(4):451-460. Epub 2020 Dec 9.

Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin150001, Heilongjiang, China.

The present study was designed to investigate the therapeutic effects of injection of dihydroartemisinin (DHA) into the balloon-injured carotid arteries on balloon injury-induced neointimal formation and to explore whether autophagy is involved in the action of DHA. Percutaneous transluminal balloon angioplasty was performed in Sprague-Dawley rats to induce neointimal formation, immediately after which DHA (100 μmol/l × 1 ml) and/or Rapamycin (1 mg/100 μl), were injected into the balloon-injured carotid arteries. After 14 days, the serum samples and carotid artery tissues were harvested for analysis. Read More

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Nutritional parameters in the blood of dams during late gestation and immediately after calving, in the umbilical vein at calving, and in the blood of calves immediately following birth in Holstein heifers pregnant with either Holstein or beef breed fetuses.

Anim Sci J 2021 Jan;92(1):e13555

Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.

Dairy cattle management lacks consideration of fetal breed, the effect of which on fetal growth and nutrition are unclear. We investigated blood parameters in 12 late-pregnant Holstein heifers with similar (Holstein, n = 5) or different (Japanese Black [n = 4] or F1 cross [n = 3]; Holstein × Japanese Black) fetus breeds and in their umbilical cords and calves. Samples were obtained from dams 1 week before calving (-1 week) and immediately after calving, from the umbilical vein at calving, and from calves immediately after birth. Read More

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January 2021