Appl Plant Sci 2021 Jan 24;9(1):e11405. Epub 2021 Jan 24.
Department of Natural Resource Management South Dakota State University 1390 College Avenue Brookings South Dakota 57007 USA.
Premise: The tallgrass prairies of North America are one of the most threatened ecosystems in the world, making efficient species identification essential for understanding and managing diversity. Here, we assess DNA barcoding with high-throughput sequencing as a method for rapid plant species identification.
Methods: Using herbarium collections representing the tallgrass prairie flora of Oak Lake Field Station, South Dakota, USA, we amplified and examined four common nuclear and plastid barcode regions (ITS, , , and ), individually and in combination, to test their success in identifying samples to family, genus, and species levels using BLAST searches of three databases of varying size. Read More