145 results match your criteria salmon hepatocytes

Structural and molecular indices in common carp (Cyprinus carpio L.) fed n-3 PUFA enriched diet.

Food Chem Toxicol 2021 Mar 23;151:112146. Epub 2021 Mar 23.

Sparos Lda, Área Empresarial de Marim, Lote C, 8700-221, Olhão, Portugal.

Sustainable freshwater aquaculture has been recently gaining attention owing to the potential of nourishing the world. The study aimed to evaluate the influence of finishing diets on the activity of 21 genes involved in hepatic lipid metabolism and intestinal homeostasis, liver and intestine histology, and the level of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids in common carp fillets. We compared two experimental diets: control diet mimicking a commercial feed (CTRL) and a test diet (CB) fortified with EPA and DHA retrieved from salmon by-products. Read More

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Safety and efficacy of astaxanthin-dimethyldisuccinate (Carophyll Stay-Pink 10%-CWS) for salmonids, crustaceans and other fish.

EFSA J 2019 Dec 18;17(12):e05920. Epub 2019 Dec 18.

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of astaxanthin-dimethyldisuccinate (ATX-DMDS) for salmonids, crustaceans and other fish. The applicant has provided evidence that ATX-DMDS currently on the market complies with the conditions of authorisation for salmon and trout. ATX and ATX-DMDS are safe for salmonids, crustaceans and fish up to 100 mg ATX/kg complete diet, corresponding to 138 mg ATX-DMDS/kg. Read More

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December 2019

Mixture toxicity of chlorpyrifos-methyl, pirimiphos-methyl, and nonylphenol in Atlantic salmon () hepatocytes.

Toxicol Rep 2020 6;7:547-558. Epub 2020 Apr 6.

Institute of Marine Research (IMR), Bergen, Norway.

Pesticide formulations typically contain adjuvants added to enhance the performance of the active ingredient. Adjuvants may modify the bioavailability and toxicity of pesticides. In this study, the aim was to examine to which degree nonylphenol (NP) may interfere with the toxicity of two organophosphorus pesticides found in aquafeeds, chlorpyrifos-methyl (CPM) and pirimiphos-methyl (PPM). Read More

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Biotransformation of phytoestrogens from soy in enzymatically characterized liver microsomes and primary hepatocytes of Atlantic salmon.

Ecotoxicol Environ Saf 2020 Jul 12;197:110611. Epub 2020 Apr 12.

Toxinology Research Group, Norwegian Veterinary Institute, 0454, Oslo, Norway. Electronic address:

Efficient aquaculture is depending on sustainable protein sources. The shortage in marine raw materials has initiated a shift to "green aquafeeds" based on staple ingredients such as soy and wheat. Plant-based diets entail new challenges regarding fish health, product quality and consumer risks due to the possible presence of chemical contaminants, natural toxins and bioactive compounds like phytoestrogens. Read More

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Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy.

Molecules 2020 Apr 12;25(8). Epub 2020 Apr 12.

Department of Biotechnology and Food Science, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway.

This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the C-enriched Ax allowed the assessment of short-time Ax metabolism. Read More

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Phoenixin-20 Stimulates mRNAs Encoding Hypothalamo-Pituitary-Gonadal Hormones, is Pro-Vitellogenic, and Promotes Oocyte Maturation in Zebrafish.

Sci Rep 2020 04 14;10(1):6264. Epub 2020 Apr 14.

Laboratory of Integrative Neuroendocrinology, Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, S7N 5B4, Saskatchewan, Canada.

Phoenixin-20 (PNX-20) is a bioactive peptide with hormone-like actions in vertebrates. In mammals, PNX stimulates hypothalamo-pituitary-gonadal hormones and regulate reproductive processes. Our immunohisto/cytochemical studies show PNX-like and the putative PNX receptor, SREB3-like immunoreactivity in the gonads of zebrafish, and in zebrafish liver (ZFL) cells. Read More

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Dissemination of (PRV-1) in Atlantic Salmon () during the Early and Regenerating Phases of Infection.

Pathogens 2020 Feb 20;9(2). Epub 2020 Feb 20.

Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, 0454 Oslo, Norway.

(PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (), but the line of events from infection, pathologic change, and regeneration has not been thoroughly described. In this study, the cellular localization and variation of PRV-1 RNA and protein levels were analyzed at different times post-exposure in experimentally infected Atlantic salmon. Immunohistochemistry, flow cytometry, and Western blot were used for assessment of the presence of the PRV-1 σ1 protein, while RT-qPCR and in situ hybridization were performed for viral RNA. Read More

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February 2020

Chlorpyrifos-induced dysfunction of lipid metabolism is not restored by supplementation of polyunsaturated fatty acids EPA and ARA in Atlantic salmon liver cells.

Toxicol In Vitro 2019 Dec 16;61:104655. Epub 2019 Sep 16.

Institute of Marine Research (IMR), Bergen, Norway.

Exposure to contaminants can lead to accumulation of lipids in the liver. This study aimed to examine whether eicosapentaenoic acid (EPA) and arachidonic acid (ARA) supplementation can protect fish cells against the negative impact of chlorpyrifos (CPF). Atlantic salmon hepatocytes were exposed to either 100 μM CPF, 200 μM EPA, 200 μM ARA, or combinations of these for 48 h, and endpoints included lipid droplet formation, gene expression, and global metabolomic analysis. Read More

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December 2019

The long-chain monounsaturated cetoleic acid improves the efficiency of the -3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells.

Br J Nutr 2019 10 3;122(7):755-768. Epub 2019 Jul 3.

Nofima, NO-1431 Ås, Norway.

The present study aimed to determine if the long-chain MUFA cetoleic acid (22 : 1n-11) can improve the capacity to synthesise the health-promoting n-3 fatty acids EPA and DHA in human and fish models. Human hepatocytes (HepG2) and salmon primary hepatocytes were first enriched with cetoleic acid, and thereafter their capacities to convert radio-labelled 18 : 3n-3 (α-linolenic acid, ALA) to EPA and DHA were measured. Increased endogenous levels of cetoleic acid led to increased production of radio-labelled EPA + DHA in HepG2 by 40 % and EPA in salmon hepatocytes by 12 %. Read More

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October 2019

Resveratrol inhibited LPS induced transcription of immune genes and secretion of eicosanoids in Atlantic salmon (Salmo salar), comparing mono-, co- and a novel triple cell culture model of head kidney leukocytes, liver cells and visceral adipocyte tissue.

Comp Biochem Physiol C Toxicol Pharmacol 2019 Oct 3;224:108560. Epub 2019 Jul 3.

Institute of Marine Research, PB 1870, Nordnes, 5817 Bergen, Norway.

The aim was to study the effect of resveratrol on the interplay of inflammatory signals using three different cell models; a metabolic organ (liver), an endocrine organ (visceral adipose tissue, VAT) and an immune organ (head kidney leukocytes, HKL) following lipopolysaccharide challenge (LPS). Atlantic salmon HKL, liver cells and VAT were isolated from the same fish (n = 5). Each cell type was cultured either as mono-cultures, as co-cultures between HKL-liver cells, liver cells-VAT and HKL-VAT. Read More

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October 2019

Europium labeled lactosylated albumin as a model workflow for the development of biotherapeutics.

Nanomedicine 2019 06 4;18:21-30. Epub 2019 Mar 4.

Chemical and Biological Technologies for Health (UTCBS), CNRS UMR8258, INSERM U1022, Paris Descartes University, Sorbonne-Paris-Cité, Chimie ParisTech, PSL Research University, School of Pharmacy, Paris, France. Electronic address:

Lactosylated albumin is currently used as a radiopharmaceutical agent to image the liver asialoglycoprotein receptors and quantify hepatic liver function in various diseases. A lactosylated protein (LACTAL) conjugate showed excellent liver uptake compared to non-lactosylated protein and a high signal to noise ratio, based on the biodistribution in mice using Tc-scintigraphy. However, in the laboratory, it is useful to have a method that can be used in daily practice to quantify cellular targeting or biodistribution. Read More

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Assessment of the targeting specificity of a fluorescent albumin conceived as a preclinical agent of the liver function.

Nanoscale 2018 Dec 8;10(45):21151-21160. Epub 2018 Nov 8.

Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), CNRS UMR8258 INSERM U1022, Université Paris Descartes, Sorbonne-Paris-Cité, Chimie ParisTech, PSL Research University, F-75006 Paris, France.

In the context of increasing liver diseases, no contrast agent is currently available in Europe and the United States to directly assess the liver function. Only neolactosylated human serum albumin is being clinically used in Asia. In order to perform preclinical studies in the context of liver diseases, we conceived a fluorescent lactosylated albumin for the quantification of liver functional cells (l-Cyal). Read More

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December 2018

Fucosterol Causes Small Changes in Lipid Storage and Brassicasterol Affects some Markers of Lipid Metabolism in Atlantic Salmon Hepatocytes.

Lipids 2018 07 27;53(7):737-747. Epub 2018 Sep 27.

Research group Requirement and Welfare, Institute of Marine Research, Nordnes gaten 50, 5005, Bergen, Norway.

Several feeding trials with Atlantic salmon fed naturally high phytosterol concentrations due to dietary rapeseed oil inclusion have shown changes in lipid metabolism and increased hepatic lipid storage in the fish. An in vitro trial with Atlantic salmon hepatocytes was, therefore, performed to study the possible direct effects of phytosterols on lipid storage and metabolism. The isolated hepatocytes were exposed to seven different sterol treatments and gene expression, as well as lipid accumulation by Oil Red O dyeing, was assessed. Read More

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Infectious Canine Hepatitis in a Brown Bear ( Ursus arctos horribilis) from Alaska, USA.

J Wildl Dis 2018 07 2;54(3):642-645. Epub 2018 Mar 2.

1 US Geological Survey, National Wildlife Health Center, 6006 Schroeder Road, Madison, Wisconsin 53711, USA.

We diagnosed infectious canine hepatitis in a free-ranging brown bear ( Ursus arctos horribilis) cub from Alaska, US, found dead in October 2015. Intranuclear inclusion bodies were present in hepatocytes, and immunohistochemistry showed reactivity to adenoviral antigens. Sequencing of the hexon protein of adenovirus showed 100% identity to canine adenovirus 1. Read More

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Metabolic effects of p,p'-DDE on Atlantic salmon hepatocytes.

J Appl Toxicol 2018 04 17;38(4):489-503. Epub 2017 Nov 17.

National Institute of Nutrition and Seafood Research (NIFES), Bergen, Norway.

Decades after being banned in many countries, DDT and its metabolites are still considered major environmental hazards. The p,p'-DDE isomer, the DDT metabolite found in highest concentration in aquaculture feeds, is an endocrine disruptor with demonstrated ability to induce epigenetic effects. This study aimed at examining the impact of p,p'-DDE on Atlantic salmon. Read More

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Metabolic signatures of bisphenol A and genistein in Atlantic salmon liver cells.

Chemosphere 2017 Dec 17;189:730-743. Epub 2017 Sep 17.

National Institute of Nutrition and Seafood Research (NIFES), Bergen, Norway.

Screening has revealed that aquafeeds with high inclusion of plant material may contain small amounts of endocrine disrupting agricultural pesticides. In this work, bisphenol A (BPA) and genistein (GEN) were selected as model endocrine disrupting toxicants with impact on DNA methylation in fish. Atlantic salmon hepatocytes were exposed in vitro to four concentrations of BPA and GEN (0. Read More

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December 2017

EPA, DHA, and Lipoic Acid Differentially Modulate the n-3 Fatty Acid Biosynthetic Pathway in Atlantic Salmon Hepatocytes.

Lipids 2017 03 28;52(3):265-283. Epub 2017 Jan 28.

Nofima (Norwegian Institute of Food, Fisheries and Aquaculture Research), P.O. Box 210, 1431, Ås, Norway.

The aim of the present study was to investigate how EPA, DHA, and lipoic acid (LA) influence the different metabolic steps in the n-3 fatty acid (FA) biosynthetic pathway in hepatocytes from Atlantic salmon fed four dietary levels (0, 0.5, 1.0 and 2. Read More

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Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats.

Mar Drugs 2016 Dec 19;14(12). Epub 2016 Dec 19.

Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan.

Dietary nucleotides play a role in maintaining the immune responses of both animals and humans. Oral administration of nucleic acids from salmon milt have physiological functions in the cellular metabolism, proliferation, differentiation, and apoptosis of human small intestinal epithelial cells. In this study, we examined the effects of DNA-rich nucleic acids prepared from salmon milt (DNSM) on the development of liver fibrosis in an in vivo ethanol-carbon tetrachloride cirrhosis model. Read More

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December 2016

Regulation of the Omega-3 Fatty Acid Biosynthetic Pathway in Atlantic Salmon Hepatocytes.

PLoS One 2016 14;11(12):e0168230. Epub 2016 Dec 14.

Nofima, Ås, Norway.

Limited availability of the n-3 fatty acids EPA and DHA have led to an interest in better understanding of the n-3 biosynthetic pathway and its regulation. The biosynthesis of alpha-linolenic acid to EPA and DHA involves several complex reaction steps including desaturation-, elongation- and peroxisomal beta-oxidation enzymes. The aims of the present experiments were to gain more knowledge on how this biosynthesis is regulated over time by different doses and fatty acid combinations. Read More

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In vitro toxicity of pirimiphos-methyl in Atlantic salmon hepatocytes.

Toxicol In Vitro 2017 Mar 12;39:1-14. Epub 2016 Nov 12.

National Institute of Nutrition and Seafood Research, Bergen, Norway.

Recent screening of Atlantic salmon feeds has uncovered residues of several pesticides, including pirimiphos-methyl. Pirimiphos-methyl is an organophosphate (OP) insecticide, causing inhibition of acetylcholinesterase in target organisms. The aim of this study was to examine pirimiphos-methyl non-targeted mode of action toxicity in Atlantic salmon using in vitro exposure. Read More

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Bioconversion of Docosapentaenoic Acid in Human Cell Lines, Caco-2, HepG2, and THP-1.

J Oleo Sci 2016 Dec 9;65(12):1017-1022. Epub 2016 Nov 9.

Faculty of Fisheries Sciences, Hokkaido University.

Docosapentaenoic acids (DPAs) are long chain polyunsaturated fatty acids that exist as two major structural isomers: n-3 DPA and n-6 DPA. n-3 DPA is found in seal meat, salmon and abalone, and n-6 DPA is found in several marine microbial oil. We investigated the bioconversion of n-3 and n-6 DPAs in three different human cell lines, Caco-2, HepG2, and THP-1. Read More

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December 2016

Enantiopure copper(II) complex of natural product rosin derivative: DNA binding, DNA cleavage and cytotoxicity.

J Biol Inorg Chem 2016 Dec 14;21(8):987-996. Epub 2016 Sep 14.

College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.

To develop chiral anticancer drug candidates for molecular target DNA, the synthesis and characterization of a novel enantiomerically pure copper(II) complex [Cu 1 Cl ] (2) of an optically pure ligand N-(pyridin-2-ylmethylene) dehydroabietylamine (1) was carried out. The coordination geometry of the copper center is a distorted square-planar arrangement. The interactions of 1 and 2 with salmon sperm DNA were investigated by viscosity measurements, UV, fluorescence and circular dichroism (CD) spectroscopic techniques. Read More

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December 2016

Minor lipid metabolic perturbations in the liver of Atlantic salmon (Salmo salar L.) caused by suboptimal dietary content of nutrients from fish oil.

Fish Physiol Biochem 2016 Oct 6;42(5):1463-80. Epub 2016 May 6.

National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029, Nordnes, 5817, Bergen, Norway.

The present study was conducted to evaluate the effects on Atlantic salmon hepatic lipid metabolism when fed diets with increasing substitution of fish oil (FO) with a vegetable oil (VO) blend. Four diets with VOs replacing 100, 90, 79 and 65 % of the FO were fed for 5 months. The levels of eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) in the experimental diets ranged from 1. Read More

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October 2016

In vitro Assessment of Hg Toxicity in Hepatocytes from Heat-Stressed Atlantic Salmon.

Biol Trace Elem Res 2016 Nov 19;174(1):226-239. Epub 2016 Apr 19.

National Institute of Nutrition and Seafood Research, Nordnesboder 1-2, N-5005, Bergen, Norway.

Global warming may alter the bioavailability of contaminants in aquatic environments. In this work, mercury (Hg) toxicity was studied in cells obtained from Atlantic salmon smolt kept at 15 °C (optimal growth temperature) for 3 months or at a stepwise increase to 20 °C (temperature-stress) during 3 months prior to cell harvest to evaluate whether acclimation temperature affects Hg toxicity. To examine possible altered dietary requirements in warmer seas, one group of fish following the stepwise temperature regimes was fed a diet spiked with antioxidants. Read More

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November 2016

Fish pre-acclimation temperature only modestly affects cadmium toxicity in Atlantic salmon hepatocytes.

J Therm Biol 2016 Apr 27;57:21-34. Epub 2016 Feb 27.

National Institute of Nutrition and Seafood Research, Nordnesboder 1-2, N-5005 Bergen, Norway.

An emerging focus in environmental toxicology is how climate change will alter bioavailability and uptake of contaminants in organisms. Ectothermic animals unable to adjust their temperature by local migration, such as farmed fish kept in net pens, may become more vulnerable to contaminants in warmer seas. The aim of this work was to study cadmium (Cd) toxicity in cells obtained from fish acclimated to sub-optimal growth temperature. Read More

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Omega-3 and alpha-tocopherol provide more protection against contaminants in novel feeds for Atlantic salmon ( L.) than omega-6 and gamma tocopherol.

Toxicol Rep 2016 14;3:211-224. Epub 2016 Jan 14.

National Institute of Nutrition and Seafood Research, Norway.

Extended use of plant ingredients in Atlantic salmon farming has increased the need for knowledge on the effects of new nutrients and contaminants in plant based feeds on fish health and nutrient-contaminant interactions. Primary Atlantic salmon hepatocytes were exposed to a mixture of PAHs and pesticides alone or in combination with the nutrients ARA, EPA, α-tocopherol, and γ-tocopherol according to a factorial design. Cells were screened for effects using xCELLigence cytotoxicity screening, NMR spectroscopy metabolomics, mass spectrometry lipidomics and RT-qPCR transcriptomics. Read More

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January 2016

The mining chemical Polydadmac is cytotoxic but does not interfere with Cu-induced toxicity in Atlantic salmon hepatocytes.

Toxicol In Vitro 2015 Dec 11;30(1 Pt B):492-505. Epub 2015 Sep 11.

National Institute of Nutrition and Seafood Research, N-5817 Bergen, Norway.

To speed up sedimentation of suspended solids the mining industry often uses flocculent chemicals. In this work we evaluated the cytotoxic and mechanistic effects of Polydadmac, and its basic component Dadmac, on fish cells. Dose-response effects, temperature-dependent effects and impact of Dadmac and Polydadmac on Cu toxicity were studied in Atlantic salmon hepatocytes. Read More

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December 2015

Evaluation of a high-EPA oil from transgenic in feeds for Atlantic salmon ( L.): Effects on tissue fatty acid composition, histology and gene expression.

Aquaculture 2015 Jul;444:1-12

Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom.

Currently, one alternative for dietary fish oil (FO) in aquafeeds is vegetable oils (VO) that are devoid of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFAs). Entirely new sources of n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids through de novo production are a potential solution to fill the gap between supply and demand of these important nutrients. was metabolically engineered to produce a seed oil (ECO) with > 20% EPA and its potential to substitute for FO in Atlantic salmon feeds was tested. Read More

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Low-Molecular-Weight Peptides from Salmon Protein Prevent Obesity-Linked Glucose Intolerance, Inflammation, and Dyslipidemia in LDLR-/-/ApoB100/100 Mice.

J Nutr 2015 Jul 20;145(7):1415-22. Epub 2015 May 20.

Department of Medicine, Quebec Heart and Lung Institute, Institute of Nutrition and Functional Foods, and

Background: We previously reported that fish proteins can alleviate metabolic syndrome (MetS) in obese animals and human subjects.

Objectives: We tested whether a salmon peptide fraction (SPF) could improve MetS in mice and explored potential mechanisms of action.

Methods: ApoB(100) only, LDL receptor knockout male mice (LDLR(-/-)/ApoB(100/100)) were fed a high-fat and -sucrose (HFS) diet (25 g/kg sucrose). Read More

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Modifying effects of vitamin E on chlorpyrifos toxicity in atlantic salmon.

PLoS One 2015 16;10(3):e0119250. Epub 2015 Mar 16.

National Institute of Nutrition and Seafood Research, Bergen, Norway.

The aim of this study was to elucidate how vitamin E (alpha tocopherol) may ameliorate the toxicity of the pesticide chlorpyrifos in Atlantic salmon. Freshly isolated hepatocytes were exposed to vitamin E, chlorpyrifos or a combination of vitamin E and chlorpyrifos (all 100 μM). Transcriptomics (RNA-seq) and metabolomics were used to screen for effects of vitamin E and chlorpyrifos. Read More

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February 2016