71,599 results match your criteria rrna gene


Effects of ferrous iron supplementation on reductive dechlorination of tetrachloroethene and on methanogenic microbial community.

FEMS Microbiol Ecol 2021 May 12. Epub 2021 May 12.

Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba, Ibaraki 305-8567, Japan.

Chloroethenes are common soil and groundwater pollutants. Their dechlorination is impacted by environmental factors, such as the presence of metal ions. We here investigated the effect of ferrous iron on bacterial reductive dechlorination of chloroethenes and on methanogen community. Read More

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Antibacterial Performance of a Streptomyces spectabilis Strain Producing Metacycloprodigiosin.

Curr Microbiol 2021 May 12. Epub 2021 May 12.

Medicine Research Institute, HuBei University of Science and Technology, Xianning, 437000, China.

After separation of bacterial colonies on solid plates, purification, and screening through the agar cup-plate method, an antibiotic-resistant bacterial isolate was obtained, and named strain L20190601, the 16S rRNA gene sequence data of strain L20190601 to GenBank, NCBI have provided GenBank accession number MW931615. 16S rRNA gene sequencing revealed that this isolate was highly similar to a number of Streptomyces species. Among them, the homology with S. Read More

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Bacteriome depiction and the trophic status of the largest Northern highland lake from Andes system: Lago de Tota, Boyacá, Colombia.

Arch Microbiol 2021 May 12. Epub 2021 May 12.

Escuela de Ciencias Biológicas, Laboratorio de Investigación en Genética Evolutiva - LIGE, L-214, Universidad Pedagógica Y Tecnológica de Colombia, Avenida Central del Norte 39-115, Tunja, Boyacá, Colombia.

Lago de Tota is the largest highland lake in Colombia and one of the most remarkable of Northern Andean Mountain range. This lake is under an anthropogenic-based eutrophication process as a consequence of non-sustainable agriculture practices developing nearby. Notable relationship between the trophic status and Bacteriome loop dynamics has been increasingly disclosed in lakes worldwide. Read More

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Impact of Vaginal Estrogen on the Urobiome in Postmenopausal Women With Recurrent Urinary Tract Infection.

Female Pelvic Med Reconstr Surg 2021 May 13. Epub 2021 May 13.

From the Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics, Gynecology, and Reproductive Sciences Biomedical Sciences Graduate Program Department of Pathology, School of Medicine Department of Pediatrics, School of Medicine Department of Bioengineering, Jacobs School of Engineering Center for Microbiome Innovation Department of Computer Science and Engineering, University of California San Diego, La Jolla, CA.

Objective: The aim of this study was to describe effects of vaginal estrogen (VE) on the urogenital microbiome in postmenopausal women with recurrent urinary tract infections (rUTIs).

Methods: This is a secondary analysis of 17 participants enrolled in a randomized controlled trial of VE versus placebo on urinary tract infection recurrence in postmenopausal women with rUTIs. Paired clean-catch urine samples were collected at baseline and after 6 months of VE and sequenced using 16S rRNA gene sequencing. Read More

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Nicotine concentration and mentholation affect bacterial community diversity in SPECTRUM research cigarettes.

Appl Microbiol Biotechnol 2021 May 11. Epub 2021 May 11.

Maryland Institute for Applied Environmental Health, University of Maryland School of Public Health, College Park, MD, USA.

Previous studies have characterized bacterial communities in menthol versus non-menthol cigarettes. However, these studies evaluated commercial cigarettes, for which levels of chemical constituents are largely unknown, and therefore, could not assess the impact of varying nicotine and menthol concentrations on tobacco bacterial communities. To address this knowledge gap, we performed time-series experiments using SPECTRUM research cigarettes with varying nicotine and menthol levels. Read More

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Extracellular Vesicles from Child Gut Microbiota Enter into Bone to Preserve Bone Mass and Strength.

Adv Sci (Weinh) 2021 05 17;8(9):2004831. Epub 2021 Feb 17.

Department of Orthopedics Xiangya Hospital Central South University Changsha Hunan 410008 China.

Recently, the gut microbiota (GM) has been shown to be a regulator of bone homeostasis and the mechanisms by which GM modulates bone mass are still being investigated. Here, it is found that colonization with GM from children (CGM) but not from the elderly (EGM) prevents decreases in bone mass and bone strength in conventionally raised, ovariectomy (OVX)-induced osteoporotic mice. 16S rRNA gene sequencing reveals that CGM reverses the OVX-induced reduction of (). Read More

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Semidry acid hydrolysis of cellulose sustained by autoclaving for production of reducing sugars for bacterial biohydrogen generation from various cellulose feedstock.

PeerJ 2021 19;9:e11244. Epub 2021 Apr 19.

Biology Department, Faculty of Science, Taibah University, Almadinah Almunawarah, Almadinah Almunawarah, Saudi Arabia.

Cellulosic biowastes are one of the cheapest and most abundant renewable organic materials on earth that can be, subsequent to hydrolysis, utilized as an organic carbon source for several fermentation biotechnologies. This study was devoted to explore a semidry acid hydrolysis of cellulose for decreasing the cost and ionic strength of the hydrolysate. For semidry acid hydrolysis, cellulose was just wetted with HCl (0 to 7 M) and subjected to autoclaving. Read More

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(Urticaceae), a new species from limestone karst in Guangxi, China.

PeerJ 2021 19;9:e11148. Epub 2021 Apr 19.

Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, China.

L.F. Fu, A. Read More

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Gut microbial ecology of the Critically Endangered Fijian crested iguana (): Effects of captivity status and host reintroduction on endogenous microbiomes.

Ecol Evol 2021 May 26;11(9):4731-4743. Epub 2021 Mar 26.

Sam Noble Oklahoma Museum of Natural History and Department of Biology University of Oklahoma Norman Oklahoma USA.

Animals often exhibit distinct microbial communities when maintained in captivity as compared to when in the wild. Such differentiation may be significant in headstart and reintroduction programs where individuals spend some time in captivity before release into native habitats. Using 16S rRNA gene sequencing, we (i) assessed differences in gut microbial communities between captive and wild Fijian crested iguanas () and (ii) resampled gut microbiota in captive iguanas released onto a native island to monitor microbiome restructuring in the wild. Read More

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Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding.

Sci Rep 2021 May 11;11(1):9936. Epub 2021 May 11.

Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.

In mammals, the intestine harbors numerous bacteria that play an important role in health. Intestinal microbiota have also been thought to be an important factor in the health of shrimp. However, the barrier systems of the digestive tracts of shrimp seem to be different from those of mammals. Read More

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Spinal Cord Injury Changes the Structure and Functional Potential of Gut Bacterial and Viral Communities.

mSystems 2021 May 11;6(3). Epub 2021 May 11.

Department of Neuroscience, The Ohio State University College of Medicine, Columbus, Ohio, USA

Emerging data indicate that gut dysbiosis contributes to many human diseases, including several comorbidities that develop after traumatic spinal cord injury (SCI). To date, all analyses of SCI-induced gut dysbiosis have used 16S rRNA amplicon sequencing. This technique has several limitations, including being susceptible to taxonomic "blind spots," primer bias, and an inability to profile microbiota functions or identify viruses. Read More

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Metagenomics and Quantitative Stable Isotope Probing Offer Insights into Metabolism of Polycyclic Aromatic Hydrocarbon Degraders in Chronically Polluted Seawater.

mSystems 2021 May 11;6(3). Epub 2021 May 11.

Department of Biological Sciences, University of Southern California, Los Angeles, California, USA

Bacterial biodegradation is a significant contributor to remineralization of polycyclic aromatic hydrocarbons (PAHs)-toxic and recalcitrant components of crude oil as well as by-products of partial combustion chronically introduced into seawater via atmospheric deposition. The Deepwater Horizon oil spill demonstrated the speed at which a seed PAH-degrading community maintained by chronic inputs responds to acute pollution. We investigated the diversity and functional potential of a similar seed community in the chronically polluted Port of Los Angeles (POLA), using stable isotope probing with naphthalene, deep-sequenced metagenomes, and carbon incorporation rate measurements at the port and in two sites in the San Pedro Channel. Read More

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The Intestinal Microbiome Primes Host Innate Immunity against Enteric Virus Systemic Infection through Type I Interferon.

mBio 2021 May 11;12(3). Epub 2021 May 11.

Key Laboratory of Animal Virology of Ministry of Agriculture, Center for Veterinary Sciences, Zhejiang University, Hangzhou, People's Republic of China

Intestinal microbiomes are of vital importance in antagonizing systemic viral infection. However, very little literature has shown whether commensal bacteria play a crucial role in protecting against enteric virus systemic infection from the aspect of modulating host innate immunity. In the present study, we utilized an enteric virus, encephalomyocarditis virus (EMCV), to inoculate mice treated with phosphate-buffered saline (PBS) or given an antibiotic cocktail (Abx) orally or intraperitoneally to examine the impact of microbiota depletion on virulence and viral replication Microbiota depletion exacerbated the mortality, neuropathogenesis, viremia, and viral burden in brains following EMCV infection. Read More

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Relationship between the gut microbiota and bile acid composition in the ileal mucosa of Crohn's disease.

Intest Res 2021 May 14. Epub 2021 May 14.

Division of Gastroenterology, Shiga University of Medical Science, Otsu, Japan.

Background/aims: Crosstalk between the gut microbiota and bile acid plays an important role in the pathogenesis of gastrointestinal disorders. We investigated the relationship between microbial structure and bile acid metabolism in the ileal mucosa of Crohn's disease (CD).

Methods: Twelve non-CD controls and 38 CD patients in clinical remission were enrolled. Read More

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Integrated gut microbiota and fecal metabolomics reveal the renoprotective effect of Rehmanniae Radix Preparata and Corni Fructus on adenine-induced CKD rats.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Apr 24;1174:122728. Epub 2021 Apr 24.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, PR China. Electronic address:

Rehmanniae Radix Preparata (RR) and Corni Fructus (CF), well-known traditional Chinese medicines (TCMs), were generally used together in the clinical practices to treat chronic kidney disease (CKD) with synergistic effects for thousands of years, but their combination mechanism remains largely unknown so far. Recent evidences have implicated intestinal flora as potential targets for the therapy of CKD. In this study, the CKD rat model was induced by adenine. Read More

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Activity and structure of methanogenic microbial communities in sediments of cascade hydropower reservoirs, Southwest China.

Sci Total Environ 2021 May 4;786:147515. Epub 2021 May 4.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China. Electronic address:

Freshwater reservoirs are an important source of the greenhouse gas methane (CH). However, little is known about the activity and structure of microbial communities involved in methanogenic decomposition of sediment organic matter (SOM) in cascade hydropower reservoirs. In this study, we targeted on sediments of three cascade reservoirs in Wujiang River, Southwest China. Read More

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Taxonomic annotation of 16S rRNA sequences of pig intestinal samples using MG-RAST and QIIME2 generated different microbiota compositions.

J Microbiol Methods 2021 May 8:106235. Epub 2021 May 8.

Scotland's Rural College, Edinburgh, UK. Electronic address:

Environmental microbiome studies rely on fast and accurate bioinformatics tools to characterize the taxonomic composition of samples based on the 16S rRNA gene. MetaGenome Rapid Annotation using Subsystem Technology (MG-RAST) and Quantitative Insights Into Microbial Ecology 2 (QIIME2) are two of the most popular tools available to perform this task. Their underlying algorithms differ in many aspects, and therefore the comparison of the pipelines provides insights into their best use and interpretation of the outcomes. Read More

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Effects of Gasterophilus pecorum infestation on the intestinal microbiota of the rewilded Przewalski's horses in China.

PLoS One 2021 11;16(5):e0251512. Epub 2021 May 11.

Department of Civil and Environmental Engineering, University of Macau, Macao, China.

Horse botflies have been a threat to the Przewalski's horses in the Kalamaili Nature Reserve in Xinjiang of China since their reintroduction to the original range. As larvae of these parasites could infest the intestine of a horse for months, they could interact with and alter the structure and composition of its intestinal microbiota, affecting adversely its health. Nonetheless, there are no such studies on the rewilded Przewalski's horses yet. Read More

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Isolation of sp. nov., a novel bacterium in the family from marine mangrove sediment.

Int J Syst Evol Microbiol 2021 May;71(5)

Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China.

A Gram-stain-negative, strictly aerobic and oval-shaped bacterial strain with a flagellum, designated GS-10, was isolated from mangrove wetland sediment. GS-10 grew at 20-40 °C (optimum, 37 °C), in the pH range of 5.0-11. Read More

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sp. nov., isolated from the respiratory tract of , and sp. nov., isolated from the lung tissue of .

Int J Syst Evol Microbiol 2021 May;71(5)

Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, PR China.

Four Gram-stain-positive, non-motile and asporous bacilli (strains ZJ-599, ZJ-621, MC1420 and MC1482), isolated from animal tissue and environmental samples collected on the Qinghai-Tibet Plateau, PR China, were taxonomically characterized. Based on the results of 16S rRNA gene sequence analyses, the closest relatives of strains ZJ-599 and ZJ-621 were LMM-1653 (97.5 %), M408/89/1 (96. Read More

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sp. nov., a slow-growing scotochromogenic species isolated from sputum.

Int J Syst Evol Microbiol 2021 May;71(5)

National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-Resistant Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, PR China.

A slow-growing, scotochromogenic mycobacterial strain (24) was isolated from the sputum of a Chinese male human. Phylogenetic analysis using the 16S rRNA gene assigned strain 24 to the complex, which includes and . The phenotypic characteristics, unique mycolic acid profile and the results of phylogenetic analysis based on and sequences strongly supported the taxonomic status of strain 24 as a representative of a species distinct from the other members of the complex. Read More

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Taishania pollutisoli gen. nov., sp. nov., Isolated from Tetrabromobisphenol A-Contaminated Soil.

Curr Microbiol 2021 May 11. Epub 2021 May 11.

Key Lab of Microbiological Engineering of Agricultural Environment, Department of Microbiology, College of Life Sciences, Ministry of Agriculture, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China.

Strain CZZ-1 was isolated from long-term TBBPA-contaminated soil Zaozhuang city, Shandong province, People's Republic of China. CZZ-1 was pink-pigmented, Gram-stain-negative, rod-shaped, non-motile and aerobic. The 16S rRNA gene analysis indicated that strain CZZ-1 shows high similarities to Fluviicola taffensis DSM 16823 (92. Read More

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Gut microbiome diversity and composition is associated with hypertension in women.

J Hypertens 2021 May 10. Epub 2021 May 10.

Department of Twin Research and Genetic Epidemiology, King's College London, London, UK Department CIBIO, University of Trento, Trento, Italy Zoe Global Limited Department of Nutritional Sciences, King's College London, London, United Kingdom Academic Rheumatology Clinical Sciences Building, Nottingham City Hospital, University of Nottingham, Nottingham, UK Both Ana M. Valdes and Cristina Menni contributed equally to this article.

Objectives: Animal studies support a role for the gut microbiota in hypertension development, but large human studies are lacking. Here, we investigated the relationship between hypertension prevalence and gut microbial composition in two cohorts.

Methods: We included 871 unrelated TwinsUK women with faecal microbiome data (16s rRNA gene sequencing). Read More

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First report of a ' Phytoplasma aurantifolia'-related strain (16SrII-V) associated with phyllody, virescence, and shoot proliferation of sweet William () in Taiwan.

Plant Dis 2021 May 11. Epub 2021 May 11.

National Chung Hsing University, 34916, Department of Plant Pathology, Taichung, Taiwan.

The sweet William () is an ornamental belonging to the Caryophyllaceae family; the species produces clusters of flowers that comes in various colors and is grown commonly as garden plants (Lim 2014). In February 2021, sweet Williams showing symptoms typical of phytoplasma diseases were found in a garden located in Wufeng District, Taichung, Taiwan (24°04'37.6"N 120°43'20. Read More

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Planococcus soli sp. nov., isolated from antarctic soil.

Antonie Van Leeuwenhoek 2021 May 11. Epub 2021 May 11.

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, 100081, Beijing, People's Republic of China.

A novel bacterial strain, designated C23, was isolated from a soil sample obtained from King George Island, Antarctica. Phenotypic, phylogenetic, chemotaxonomic and molecular analyses were performed on the new isolate. Strain C23 formed orange colonies on agar plates and was Gram-stain-positive. Read More

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A novel metabarcoding primer pair for environmental DNA analysis of Cephalopoda (Mollusca) targeting the nuclear 18S rRNA region.

R Soc Open Sci 2021 Feb 10;8(2):201388. Epub 2021 Feb 10.

Marine Evolutionary Ecology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.

Cephalopods are pivotal components of marine food webs, but biodiversity studies are hampered by challenges to sample these agile marine molluscs. Metabarcoding of environmental DNA (eDNA) is a potentially powerful technique to study oceanic cephalopod biodiversity and distribution but has not been applied thus far. We present a novel universal primer pair for metabarcoding cephalopods from eDNA, (Forward: 5'-CGC GGC GCT ACA TAT TAG AC-3', Reverse: 5'-GCA CTT AAC CGA CCG TCG AC-3'). Read More

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February 2021

The preliminary investigation of potential response biomarkers to PAHs exposure on childhood asthma.

J Expo Sci Environ Epidemiol 2021 May 10. Epub 2021 May 10.

The Key Laboratory of Modern Toxicology of Ministry of Education and Department of Health Inspection and Quarantine, Nanjing Medical University, Nanjing, China.

Background: Exposure to polycyclic aromatic hydrocarbons (PAHs) is a potential risk factor for asthma prevalence. This study aims to explore whether PAHs exposure is associated with childhood asthma by altering microbial diversity and metabolic profiles.

Methods: Thirty children with asthma and 30 children as control in Nanjing, China were recruited. Read More

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Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples.

Sci Rep 2021 May 10;11(1):9859. Epub 2021 May 10.

Department of Pharmaceutics, School of Pharmaceutical Sciences & Research, Jamia Hamdard, New Delhi, India.

Leprosy, a progressive, mutilating and highly stigmatized disease caused by Mycobacterium leprae (ML), continues to prevail in the developing world. This is due to the absence of rapid, specific and sensitive diagnostic tools for its early detection since the disease gets notified only with the advent of physical scarring in patients. This study reports the development of a Loop-mediated isothermal amplification (LAMP) technique for fast, sensitive and specific amplification of 16S rRNA gene of ML DNA for early detection of leprosy in resource-limited areas. Read More

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The epidemiology of amoebiasis in Thi-Qar province, Iraq (2015-2020): Differentiation of Entamoeba histolytica/Entamoeba dispar using nested PCR and real-time PCR.

Epidemiol Health 2021 May 6:e2021034. Epub 2021 May 6.

Bint Al-Huda Maternity and Children Teaching Hospital, Thi-Qar Health Office, Nasiriyah 64001, Iraq.

Objectives: The objective was to evaluate the present status of amoebiasis in Thi-Qar province, south of Iraq. As well a determination of Entamoeba histolytica and Entamoeba dispar prevalence with nested and real time PCR.

Methods: Epidemiological data was taken from public health department/ Thi-Qar Health Office (2015-2020). Read More

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Aquatic microbial community is partially functionally redundant: Insights from an in situ reciprocal transplant experiment.

Sci Total Environ 2021 Apr 30;786:147433. Epub 2021 Apr 30.

Biodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan; Bioinformatics Program, Institute of Information Science, Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan. Electronic address:

Microbial communities are considered to be functionally redundant, but few studies have tested this hypothesis empirically. In this study, we performed an in situ reciprocal transplant experiment on the surface and bottom waters of two lakes (Tsuei-Feng (T) and Yuan-Yang (Y)) with disparate trophic states and tracked changes in their microbial community composition and functions for 6 weeks using high-throughput sequencing and functional approaches. T lake's surface (Ts) and bottom (Tb) water active bacterial community (16S rRNA gene-transcript) was dominated by Actinobacteria, Bacteroidia, and Cyanobacteria, whereas Y lake's surface (Ys) and bottom (Yb) water had Gammaproteobacteria, Alphaproteobacteria, and Bacteroidia as the dominant classes. Read More

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