Plant Physiol 2015 Jan 25;167(1):153-63. Epub 2014 Nov 25.
Golan Research Institute, University of Haifa, Qatzrin 12900, Israel (M.D.-Y., R.D.);Noga AgroTech Desert Agriculture, Kmehin 85511, Israel (A.L.);Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 (R.D., T.T.); andDepartment of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 84105, Israel (T.T.).
Agrobacterium tumefaciens delivers its single-stranded transferred DNA (T-strand) into the host cell nucleus, where it can be converted into double-stranded molecules. Various studies have revealed that double-stranded transfer DNA (T-DNA) intermediates can serve as substrates by as yet uncharacterized integration machinery. Nevertheless, the possibility that T-strands are themselves substrates for integration cannot be ruled out. Read More