4,806 results match your criteria rose bengal

Cathodic electrodeposition of MOF films using hydrogen peroxide.

Angew Chem Int Ed Engl 2021 Sep 20. Epub 2021 Sep 20.

KUL: Katholieke Universiteit Leuven, Department of materials engineering, BELGIUM.

Metal-organic framework (MOF) films can be made by cathodic electrodeposition, where a Brønsted base is formed electrochemically which deprotonates the MOF linkers that are present in solution as undissociated/partially dissociated weak acids. However, the co-deposition of metal and the narrow range of possible metal nodes limit the scope of this method. In this work, we propose the use of hydrogen peroxide (hydrogen peroxide assisted cathodic deposition or HPACD), to overcome these limitations. Read More

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September 2021

Photodynamic Therapy for Infectious Keratitis.

Curr Ophthalmol Rep 2020 Dec 12;8:245-251. Epub 2020 Sep 12.

Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, USA.

Infectious keratitis is a sight-threatening microbial infection. The prevalence of antimicrobial resistance in cases of infectious keratitis has increased the demand for fortified compounded antimicrobial drops. Even with proper medical management, severe cases of infectious keratitis can further evolve into corneal perforation, requiring surgical intervention in the form of keratoplasty to control the infectious process. Read More

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December 2020

Epidemiology of bovine brucellosis in Hisar, India: identification of risk factors and assessment of knowledge, attitudes, and practices among livestock owners.

Trop Anim Health Prod 2021 Sep 17;53(5):450. Epub 2021 Sep 17.

Veterinary Epidemiology, Economics and Public Health Group, Department of Pathobiology and Population Sciences, The Royal Veterinary College, Hertfordshire, AL9 7TA, UK.

Brucellosis caused by facultative intracellular bacteria, Brucella, remains a global threat to both animal and human health. In this study we aimed to identify potential risk factors of bovine brucellosis and to assess the knowledge, attitudes, and practices (KAPs) of livestock keepers in Hisar, India. A standardized questionnaire was used to collate information regarding potential risk factors of bovine brucellosis and livestock owners' KAPs. Read More

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September 2021

Activating a DNA Nanomachine via Computation across Cancer Cell Membranes for Precise Therapy of Solid Tumors.

J Am Chem Soc 2021 Sep 13. Epub 2021 Sep 13.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Taking advantage of cancer cells' endogenous characters, the responsive activation of DNA nanomachines has achieved great success in tumor therapy. Combining with extra stimuli such as external light irradiation provided spatiotemporal control of DNA nanomachine activation. However, specific activation at the cellular level is still challenging considering the macroscopic-scale exposure area of usual light sources. Read More

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September 2021

Seroprevalence trend of human brucellosis and MLVA genotyping characteristics of Brucella melitensis in Shaanxi Province, China, during 2008-2020.

Transbound Emerg Dis 2021 Sep 12. Epub 2021 Sep 12.

Department of Plague and Brucellosis, Shaanxi Center for Disease Control and Prevention, Xi' an, 710054, China.

In this study, a total of 179,907 blood samples from populations with suspected Brucella spp. infections were collected between 2008 and 2020 and analyzed by the Rose-Bengal plate test (RBPT) and serum agglutination test (SAT). Moreover, conventional biotyping, B. Read More

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September 2021

Phenotypic and molecular identification of Brucella suis biotype 1 in a pig from Brazil-case report.

Braz J Microbiol 2021 Sep 11. Epub 2021 Sep 11.

Department of Animal Production and Preventive Veterinary Medicine, Faculty of Veterinary Medicine and Zootechnics, State University of Sao Paulo, Botucatu, SP, Brazil.

Brucellosis is a zoonotic disease with a global impact. Brucella suis is one of the most pathogenic species to humans, requiring different measures for the control and/or eradication of the disease. The serological investigation for brucellosis was performed in pigs, horses, dogs, and cattle on a farm with a history of abortion in sows and necropsy of a boar with severe necrosuppurative orchitis. Read More

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September 2021

Prevalence and risk factors of human brucellosis and malaria among patients with fever in malaria-endemic areas, attending health institutes in Awra and Gulina district, Afar Region, Ethiopia.

BMC Infect Dis 2021 Sep 10;21(1):942. Epub 2021 Sep 10.

Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia.

Background: Brucellosis is an important neglected bacterial zoonotic disease that has been affecting animals and humans for decades. Malaria has been considered major cause of illness in tropical areas, including Ethiopia. This study aimed to identify prevalence and risk factors of human brucellosis and malaria among patients with fever in malaria-endemic areas attending health institutes in Awra and Gulina district, Afar Region, Ethiopia. Read More

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September 2021

Carotenoids Do Not Protect Bacteriochlorophylls in Isolated Light-Harvesting LH2 Complexes of Photosynthetic Bacteria from Destructive Interactions with Singlet Oxygen.

Molecules 2021 Aug 24;26(17). Epub 2021 Aug 24.

Institute of Basic Biological Problems RAS, 142290 Pushchino, Russia.

The effect of singlet oxygen on light-harvesting (LH) complexes has been studied for a number of sulfur (S) and nonsulfur (S) photosynthetic bacteria. The visible/near-IR absorption spectra of the standard LH2 complexes (B800-850) of (.) (S), (. Read More

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Fate and mitigation of Salmonella contaminated in lettuce (Lactuca sativa) seeds grown in a hydroponic system.

J Appl Microbiol 2021 Sep 8. Epub 2021 Sep 8.

Department of Food Science & Technology, Faculty of Science, National University of Singapore, Singapore, Singapore.

Aims: We investigated the fate of Salmonella in lettuce seeds grown in a hydroponic system and the potentials of applying photodynamic inactivation (PDI) to enhance microbial safety of hydroponic farming systems.

Methods And Results: Lettuce was grown from Salmonella-contaminated seeds, and rose bengal-mediated PDI was applied. Without intervention, Salmonella could persist in plants and hydroponic farming environment throughout 6 weeks of lettuce growth. Read More

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September 2021

Whole genome sequencing of colonies derived from cannabis flowers and the impact of media selection on benchmarking total yeast and mold detection tools.

F1000Res 2021 21;10:624. Epub 2021 Jul 21.

Research and Development, Medicinal Genomics, Beverly, Mass, 01915, USA.

Background: Cannabis products are subjected to microbial testing for human pathogenic fungi and bacteria. These testing requirements often rely on non-specific colony forming unit (CFU/g) specifications without clarity on which medium, selection or growth times are required. We performed whole genome sequencing to assess the specificity of colony forming units (CFU) derived from three different plating media: Potato Dextrose Agar (PDA), PDA with chloramphenicol and Dichloran Rose Bengal with chloramphenicol (DRBC). Read More

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Mimicking the energy funnel of the photosynthetic unit using a dendrimer-dye supramolecular assembly.

Chem Asian J 2021 Sep 6. Epub 2021 Sep 6.

Indian Institute of Science Education and Research, Department of Chemical Sciences, IISER Kolkata, 741246, Mohanpur, INDIA.

Photosynthesis involves light-harvesting complexes where an array of antenna pigment channels the absorbed solar energy to the reaction centre of a photosystem. This work reports a supramolecular dendrimer-dye assembly that mimics the natural light-harvesting mechanism. A dendrimeric molecule based on two-fluorophores has been constructed with three coumarin units at the end of three long arms and a 7-diethylaminocoumarin unit at the interior. Read More

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September 2021

Documenting the absence of bovine brucellosis in dairy cattle herds in the southern region of Malawi and the associated knowledge, attitudes and practices of farmers.

J S Afr Vet Assoc 2021 Aug 3;92(0):e1-e7. Epub 2021 Aug 3.

Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia; and, Department of Animal Health and Livestock Development, Ministry of Agriculture and Food Security, Malawi Government, Lilongwe.

There is paucity of Brucella prevalence data in Malawi. For this reason, a cross-sectional study was conducted, from 06 January 2020 to 27 February 2020, to estimate the seroprevalence of brucellosis in dairy cattle herds amongst smallholder farmers, government and private dairy farms in the southern region. A total of 529 serum samples were screened for anti-Brucella antibodies using the Rose Bengal test (RBT) and a competitive enzyme-linked immunosorbent assay (cELISA). Read More

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Cost-Benefit Analysis of a Mass Vaccination Strategy to Control Brucellosis in Sheep and Goats in Northern Iraq.

Vaccines (Basel) 2021 Aug 8;9(8). Epub 2021 Aug 8.

School of Veterinary Medicine, Murdoch University, Perth 6150, Australia.

Brucellosis is a major economic and production-limiting disease for livestock owners and the community in Iraq. A cost-benefit analysis was conducted to evaluate the impact of an expanded annual mass vaccination programme of sheep and goats that involves all female and male sheep and goats over the age of 3 months with Rev. 1 vaccine. Read More

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Chromophore of an Enhanced Green Fluorescent Protein Can Play a Photoprotective Role Due to Photobleaching.

Int J Mol Sci 2021 Aug 9;22(16). Epub 2021 Aug 9.

Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, 02-093 Warsaw, Poland.

Under stress conditions, elevated levels of cellular reactive oxygen species (ROS) may impair crucial cellular structures. To counteract the resulting oxidative damage, living cells are equipped with several defense mechanisms, including photoprotective functions of specific proteins. Here, we discuss the plausible ROS scavenging mechanisms by the enhanced green fluorescent protein, EGFP. Read More

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Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania.

PLoS Negl Trop Dis 2021 Aug 24;15(8):e0009630. Epub 2021 Aug 24.

Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom.

Background: Brucellosis is a neglected zoonosis endemic in many countries, including regions of sub-Saharan Africa. Evaluated diagnostic tools for the detection of exposure to Brucella spp. are important for disease surveillance and guiding prevention and control activities. Read More

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Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability.

J Vis Exp 2021 Aug 6(174). Epub 2021 Aug 6.

Department of Pathology & Cell Biology; Vagelos College of Physicians and Surgeons, Columbia University; Department of Neurology; Vagelos College of Physicians and Surgeons, Columbia University; The Taub Institute for Research on Alzheimer's Disease and the Aging Brain; Vagelos College of Physicians and Surgeons, Columbia University;

Mouse models of retinal vein occlusion (RVO) are often used in ophthalmology to study hypoxic-ischemic injury in the neural retina. In this report, a detailed method pointing out critical steps is provided with recommendations for optimization to achieve consistently successful occlusion rates across different genetically modified mouse strains. The RVO mouse model consists primarily of the intravenous administration of a photosensitizer dye followed by laser photocoagulation using a retinal imaging microscope attached to an ophthalmic guided laser. Read More

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Is there an association between zinc and COVID-19-associated mucormycosis? Results of an experimental and clinical study.

Mycoses 2021 Oct 1;64(10):1291-1297. Epub 2021 Sep 1.

Department of Pulmonary Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India.

Background: The enormous increase in COVID-19-associated mucormycosis (CAM) in India lacks an explanation. Zinc supplementation during COVID-19 management is speculated as a contributor to mucormycosis. We conducted an experimental and clinical study to explore the association of zinc and mucormycosis. Read More

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October 2021

Rift Valley fever and Brucella spp. in ruminants, Somalia.

BMC Vet Res 2021 Aug 21;17(1):280. Epub 2021 Aug 21.

Vector-Borne Diseases Laboratory, Department of Veterinary Medicine, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.

Background: Fourteen-years after the last Rift Valley fever (RVF) virus (RVFV) outbreak, Somalia still suffers from preventable transboundary diseases. The tradition of unheated milk consumption and handling of aborted materials poses a public health risk for zoonotic diseases. Limited data are available on RVF and Brucella spp. Read More

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Isolation and sero-genomo-epidemiological studies on Brucella infection in dairy cattle in Meghalaya, India.

Comp Immunol Microbiol Infect Dis 2021 Oct 5;78:101694. Epub 2021 Aug 5.

Division of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umroi Road, Umiam, Meghalaya, 793103, India. Electronic address:

In this study, we report the serological, bacteriological and whole genome sequencing data of a 6 years study of Brucella abortus in Meghalaya, India. Investigation of 3060 sera samples indicated overall prevalence of 6.4% by Rose Bengal Plate Test and 10. Read More

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October 2021

Toward Universal Photodynamic Coatings for Infection Control.

Front Med (Lausanne) 2021 28;8:657837. Epub 2021 Jul 28.

Department of Chemistry, North Carolina State University, Raleigh, NC, United States.

The dual threats posed by the COVID-19 pandemic and hospital-acquired infections (HAIs) have emphasized the urgent need for self-disinfecting materials for infection control. Despite their highly potent antimicrobial activity, the adoption of photoactive materials to reduce infection transmission in hospitals and related healthcare facilities has been severely hampered by the lack of scalable and cost-effective manufacturing, in which case high-volume production methods for fabricating aPDI-based materials are needed. To address this issue here, we examined the antimicrobial efficacy of a simple bicomponent spray coating composed of the commercially-available UV-photocrosslinkable polymer -methyl-4(4'-formyl-styryl)pyridinium methosulfate acetal poly(vinyl alcohol) (SbQ-PVA) and one of three aPDI photosensitizers (PSs): zinc-tetra(4--methylpyridyl)porphine (ZnTMPyP), methylene blue (MB), and Rose Bengal (RB). Read More

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Knowledge and perception of community about causes of cattle abortion and case-control study of brucellosis as cause of abortion in Jimma zone, Ethiopia.

Vet Med Sci 2021 Aug 14. Epub 2021 Aug 14.

School of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia.

Background: Abortion, causes by several infectious and non-infectious factors is one of the most critical health problems of cattle in Ethiopia. Thus, this study aimed to assess knowledge and perception of the community about potential causes of abortion and case-control study of brucellosis as a cause of abortion in cattle of study districts.

Methods: A cross-sectional study was conducted between October 2016 and October 2017 in selected districts of the Jimma zone using a questionnaire survey. Read More

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A screening method for decoloration of xenobiotic dyes by fungi.

J Microbiol Methods 2021 09 10;188:106301. Epub 2021 Aug 10.

Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584, CH, Utrecht, the Netherlands. Electronic address:

Wood degrading fungi are often screened for their ability to degrade xenobiotics such as dyes. Dye decoloration by these fungi on solid media could until now only be assessed qualitatively. We here describe a fast quantitative method to screen for dye decoloration on such media. Read More

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September 2021

Designer exosomes for targeted and efficient ferroptosis induction in cancer via chemo-photodynamic therapy.

Theranostics 2021 13;11(17):8185-8196. Epub 2021 Jul 13.

State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China.

Efficient and specific induction of cell death in liver cancer is urgently needed. In this study, we aimed to design an exosome-based platform to deliver ferroptosis inducer (Erastin, Er) and photosensitizer (Rose Bengal, RB) into tumor tissues with high specificity. Exosome donor cells (HEK293T) were transfected with control or CD47-overexpressing plasmid. Read More

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Quenching effects of (-)-Epigallocatechin gallate for singlet oxygen production and its protection against oxidative damage induced by Ce6-mediated photodynamic therapy in vitro.

Photodiagnosis Photodyn Ther 2021 Jul 29;36:102467. Epub 2021 Jul 29.

Key Laboratory of OptoElectronic Science and Technology for Medicine of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou 350007, PR China. Electronic address:

Background: Singlet oxygen (O) is highly reactive to biological components such as lipids, proteins and DNA, which induces oxidative damage to cells and tissues. Natural antioxidants may function as O quencher to prevent O involved photosensitized oxidation in biological system.

Methods: Time-resolved measurement of O luminescence was employed to evaluate the O quenching abilities of natural antioxidants in air-statured phosphate buffered saline (PBS), including (-)-Epigallocatechin gallate (EGCG), Proanthocyanidins, L-carnosine and Vitamin C. Read More

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Photodynamic Inactivation of by PHEMA Films Loaded with Rose Bengal: Potentiation Effect of Potassium Iodide.

Polymers (Basel) 2021 Jul 6;13(14). Epub 2021 Jul 6.

Departamento de Química Inorgánica y Orgánica, Universitat Jaume I, Av. V. Sos Baynat s/n, 12071 Castellón, Spain.

Four formulations have been used to produce different poly(2-hydroxyethyl methacrylate) (PHEMA) thin films, containing singlet oxygen photosensitizer Rose Bengal (). The polymers have been characterized employing Thermogravimetric Analysis (TGA), Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) and UV-vis Absorption Spectroscopy. When irradiated with white light (400-700 nm) films generated singlet oxygen (O), as demonstrated by the reactivity with O trap 9,10-dimethylanthracene (). Read More

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Rose Bengal and Future Directions in Larynx Tumor Photodynamic Therapy.

Photochem Photobiol 2021 Jul 20. Epub 2021 Jul 20.

Medical College of The University of Rzeszow, University of Rzeszów, Rzeszów, Poland.

Photodynamic Therapy (PDT) seems to be a promising method in the treatment of larynx tumor tissues. The aim of the present analysis was the study of photosensitizer penetration of larynx tissue associated with the application of PDT in vitro. This study is based on the use of photosensitive compounds Rose Bengal (RB) that selectively accumulate in larynx tissue. Read More

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Seroprevalence of brucellosis and Q fever infections amongst pastoralists and their cattle herds in Sokoto State, Nigeria.

PLoS One 2021 20;16(7):e0254530. Epub 2021 Jul 20.

Department of Veterinary Public Health and Preventive Medicine, University of Ibadan, Ibadan, Nigeria.

Brucellosis and Q fever are neglected zoonoses of global health importance, with unknown true prevalence in occupationally vulnerable settings, partly due to misdiagnosis for other febrile conditions and poor access to primary health care. We examined the seroprevalence of these diseases and associated factors amongst pastoralists and their cattle in Sokoto State, a hub of cattle and pastoral populations in Nigeria. Serum samples randomly collected from 137 pastoralists and 366 cattle from 27 herds in three selected Local Government Areas (LGAs) in the state were analysed for antibodies to Brucella abortus using Rose Bengal Plate Test (RBT) and competitive Enzyme-Linked Immunosorbent Assay (cELISA) as well as antibodies to Coxiella burnetti using indirect ELISA. Read More

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Visible light-catalyzed fluoroalkylation reactions of free aniline derivatives.

Photochem Photobiol Sci 2021 Aug 20;20(8):971-983. Epub 2021 Jul 20.

Departamento de Ciencias Químicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954, CP 1113, Buenos Aires, Argentina.

The electron-rich nature of aminoaromatic compounds and the electrophilic character of fluoroalkyl R radicals allow for a special match in substitution reactions. We herein present visible light photocatalyzed fluoroalkylation reactions of aniline derivatives, with a study of the reaction mechanisms. The examples evaluated make use of different photocatalysts, such as polypyridyl complexes of Ir or Ru transition metals, organic dyes such as Rose Bengal, phthalocyanine-metal organocatalysts, or visible-light activated complexes. Read More

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