458 results match your criteria role ogg1


NEIL1 and NEIL2 Are Recruited as Potential Backup for OGG1 upon OGG1 Depletion or Inhibition by TH5487.

Int J Mol Sci 2021 Apr 27;22(9). Epub 2021 Apr 27.

Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, 171 65 Stockholm, Sweden.

DNA damage caused by reactive oxygen species may result in genetic mutations or cell death. Base excision repair (BER) is the major pathway that repairs DNA oxidative damage in order to maintain genomic integrity. In mammals, eleven DNA glycosylases have been reported to initiate BER, where each recognizes a few related DNA substrate lesions with some degree of overlapping specificity. Read More

View Article and Full-Text PDF

[GO System, a DNA Repair Pathway to Cope with Oxidative Damage].

Mol Biol (Mosk) 2021 Mar-Apr;55(2):223-242

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia.

The GO system is part of the DNA base excision repair pathway and is required for the error-free repair of 8-oxoguanine (oxoG), one of the most common oxidative DNA lesions. Due to the ability of oxoG to form oxoG:A mispairs, this base is highly mutagenic. Its repair requires the action of two enzymes: 8-oxoguanine DNA glycosylase (Fpg or MutM in bacteria and OGG1 in eukaryotes), which removes oxoG from oxoG:C pairs, and adenine DNA glycosylase (MutY in bacteria and MUTYH in eukaryotes), which removes A from oxoG:A mispairs to prevent mutations. Read More

View Article and Full-Text PDF

DNA damage and oxidative stress in human cells infected by Trypanosoma cruzi.

PLoS Pathog 2021 Apr 7;17(4):e1009502. Epub 2021 Apr 7.

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Trypanosoma cruzi is the etiologic agent of Chagas' disease. Infected cells with T. cruzi activate several responses that promote unbalance of reactive oxygen species (ROS) that may cause DNA damage that activate cellular responses including DNA repair processes. Read More

View Article and Full-Text PDF

Vitamin D changes expression of DNA repair genes in the patients with multiple sclerosis.

Gene 2021 May 13;781:145488. Epub 2021 Feb 13.

Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:

Oxidative stress (OS) plays an essential role in demyelination and tissue injury related to pathogenesis of multiple sclerosis (MS). On the other hand, vitamin D (VD) as an antioxidant reduces oxidative stress and has been used as adjuvant therapy in autoimmune diseases. Although VD supplementation is suggested as a protective and immunomodulation factor for MS patients, the molecular mechanisms remain unclear. Read More

View Article and Full-Text PDF

A Novel Role for the DNA Repair Enzyme 8-Oxoguanine DNA Glycosylase in Adipogenesis.

Int J Mol Sci 2021 Jan 25;22(3). Epub 2021 Jan 25.

Rutgers Center for Lipid Research, New Jersey Institute for Food, Nutrition, and Health, Rutgers University, New Brunswick, NJ 08901, USA.

Cells sustain constant oxidative stress from both exogenous and endogenous sources. When unmitigated by antioxidant defenses, reactive oxygen species damage cellular macromolecules, including DNA. Oxidative lesions in both nuclear and mitochondrial DNA are repaired via the base excision repair (BER) pathway, initiated by DNA glycosylases. Read More

View Article and Full-Text PDF
January 2021

Effect of DNA Glycosylases OGG1 and Neil1 on Oxidized G-Rich Motif in the Promoter.

Int J Mol Sci 2021 Jan 24;22(3). Epub 2021 Jan 24.

Laboratory of Biochemistry, Department of Medicine, P.le Kolbe 4, 33100 Udine, Italy.

The promoter of the () proto-oncogene contains, upstream of the transcription start site, a quadruplex-forming motif called 32R with regulatory functions. As guanine under oxidative stress can be oxidized to 8-oxoguanine (8OG), we investigated the capacity of glycosylases 8-oxoguanine glycosylase (OGG1) and endonuclease VIII-like 1 (Neil1) to excise 8OG from 32R, either in duplex or G-quadruplex (G4) conformation. We found that OGG1 efficiently excised 8OG from oxidized 32R in duplex but not in G4 conformation. Read More

View Article and Full-Text PDF
January 2021

TET-mediated DNA demethylation plays an important role in arsenic-induced HBE cells oxidative stress via regulating promoter methylation of OGG1 and GSTP1.

Toxicol In Vitro 2021 Apr 1;72:105075. Epub 2021 Jan 1.

The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang, 550025, Guizhou, PR China. Electronic address:

Environmental exposure to arsenic remains a worldwide public health challenge. Oxidative stress and aberrant DNA methylation are both characteristics of arsenic toxicology; however, the relationship between these is not well understood. Ten-eleven translocation (TET1, TET2 and TET3), which is the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), plays a central role in the DNA demethylation process. Read More

View Article and Full-Text PDF

Lesion Recognition and Cleavage of Damage-Containing Quadruplexes and Bulged Structures by DNA Glycosylases.

Front Cell Dev Biol 2020 30;8:595687. Epub 2020 Nov 30.

Institute of Chemical Biology and Fundamental Medicine of SB RAS, Novosibirsk, Russia.

Human telomeres as well as more than 40% of human genes near the promoter regions have been found to contain the sequence that may form a G-quadruplex structure. Other non-canonical DNA structures comprising bulges, hairpins, or bubbles may have a functionally important role during transcription, replication, or recombination. The guanine-rich regions of DNA are hotspots of oxidation that forms 7,8-dihydro-8-oxoguanine, thymine glycol, and abasic sites: the lesions that are handled by the base excision repair pathway. Read More

View Article and Full-Text PDF
November 2020

The E3 Ubiquitin Ligase NEDD4L Targets OGG1 for Ubiquitylation and Modulates the Cellular DNA Damage Response.

Front Cell Dev Biol 2020 12;8:607060. Epub 2020 Nov 12.

Cancer Research Centre, Department of Molecular and Clinical Cancer Medicine, University of Liverpool, Liverpool, United Kingdom.

8-Oxoguanine DNA glycosylase (OGG1) is the major cellular enzyme required for the excision of 8-oxoguanine DNA base lesions in DNA through the base excision repair (BER) pathway, and therefore plays a major role in suppressing mutagenesis and in controlling genome stability. However, the mechanism of regulation of cellular OGG1 protein, particularly in response to oxidative stress, is unclear. We have purified the major E3 ubiquitin ligase responsible for OGG1 ubiquitylation from human cell extracts, and identify this as E3 ubiquitin-protein ligase NEDD4-like (NEDD4L). Read More

View Article and Full-Text PDF
November 2020

Polymorphisms in the BER and NER pathways and their influence on survival and toxicity in never-smokers with lung cancer.

Sci Rep 2020 12 3;10(1):21147. Epub 2020 Dec 3.

Department of Preventive Medicine and Public Health, University of Santiago de Compostela, C/San Francisco s/n, 15782, Santiago de Compostela, Spain.

Polymorphisms in DNA repair pathways may play a relevant role in lung cancer survival in never-smokers. Furthermore, they could be implicated in the response to chemotherapy and toxicity of platinum agents. The aim of this study was to evaluate the influence of various genetic polymorphisms in the BER and NER DNA repair pathways on survival and toxicity in never-smoker LC patients. Read More

View Article and Full-Text PDF
December 2020

The role of cysteines in the structure and function of OGG1.

J Biol Chem 2020 Nov 17. Epub 2020 Nov 17.

Cellular and Molecular Medicine, University of Arizona College of Medicine, United States.

8-oxoguanine glycosylase (OGG1) is a base excision repair enzyme responsible for the recognition and removal of 8-oxoguanine, a commonly occurring oxidized DNA modification. OGG1 prevents the accumulation of mutations and regulates the transcription of various oxidative stress-response genes. In addition to targeting DNA, oxidative stress can affect proteins like OGG1 itself, specifically at cysteine residues. Read More

View Article and Full-Text PDF
November 2020

Lost in the Crowd: How Does Human 8-Oxoguanine DNA Glycosylase 1 (OGG1) Find 8-Oxoguanine in the Genome?

Int J Mol Sci 2020 Nov 7;21(21). Epub 2020 Nov 7.

Institute of Cellular and Molecular Radiobiology, Institut de Biologie François Jacob, CEA, Université Paris-Saclay, Université de Paris, 18 route du Panorama, F-92265 Fontenay-aux-Roses, France.

The most frequent DNA lesion resulting from an oxidative stress is 7,8-dihydro-8-oxoguanine (8-oxoG). 8-oxoG is a premutagenic base modification due to its capacity to pair with adenine. Thus, the repair of 8-oxoG is critical for the preservation of the genetic information. Read More

View Article and Full-Text PDF
November 2020

Epigenetic silencing of TET1 mediated hydroxymethylation of base excision repair pathway during lung carcinogenesis.

Environ Pollut 2021 Jan 15;268(Pt B):115860. Epub 2020 Oct 15.

Institute of Toxicology, College of Preventive Medicine, Third Military Medical University (Army Medical University), Chongqing, 400038, PR China. Electronic address:

The methylcytosine dioxygenase Ten-eleven translocation 1 (TET1) is an important regulator for the balance of DNA methylation and hydroxymethylation through various pathways. Increasing evidence has suggested that TET1 probably involved in DNA methylation and demethylation dysregulation during chemical carcinogenesis. However, the role and mechanism of TET1 during lung cancer remains unclear. Read More

View Article and Full-Text PDF
January 2021

OGG1 Inhibitor TH5487 Alters OGG1 Chromatin Dynamics and Prevents Incisions.

Biomolecules 2020 10 26;10(11). Epub 2020 Oct 26.

Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, 171 77 Stockholm, Sweden.

8-oxoguanine DNA glycosylase (OGG1) is the main DNA glycosylase responsible for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG) from duplex DNA to initiate base excision repair. This glycosylase activity is relevant in many pathological conditions including cancer, inflammation, and neurodegenerative diseases. To have a better understanding of the role of OGG1, we previously reported TH5487, a potent active site inhibitor of OGG1. Read More

View Article and Full-Text PDF
October 2020

Astaxanthin protects oxidative stress mediated DNA damage and enhances longevity in Saccharomyces cerevisiae.

Biogerontology 2021 Feb 27;22(1):81-100. Epub 2020 Oct 27.

Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry, 605014, India.

Reactive oxygen species (ROS) have long been found to play an important role in oxidative mediated DNA damage. Fortunately, cells possess an antioxidant system that can neutralize ROS. However, oxidative stress occurs when antioxidants are overwhelmed by ROS or impaired antioxidant pathways. Read More

View Article and Full-Text PDF
February 2021

The Effect of Allopregnanolone on Enzymatic Activity of the DNA Base Excision Repair Pathway in the Sheep Hippocampus and Amygdala under Natural and Stressful Conditions.

Int J Mol Sci 2020 Oct 20;21(20). Epub 2020 Oct 20.

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland.

The neurosteroid allopregnanolone (AL) has many beneficial functions in the brain. This study tested the hypothesis that AL administered for three days into the third brain ventricle would affect the enzymatic activity of the DNA base excision repair (BER) pathway in the hippocampal CA1 and CA3 fields and the central amygdala in luteal-phase sheep under both natural and stressful conditions. Acute stressful stimuli, including isolation and partial movement restriction, were used on the last day of infusion. Read More

View Article and Full-Text PDF
October 2020

ROS-dependent DNA damage and repair during germination of NaCl primed seeds.

J Photochem Photobiol B 2020 Dec 10;213:112050. Epub 2020 Oct 10.

Department of Plant Sciences, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India. Electronic address:

Reactive oxygen species (ROS) generated during rehydration of seeds is a major source of cellular damage. Successful germination depends on maintaining the oxidative window and ability of the cells to repair the DNA damage accumulated during seed developmental process, maturational drying, and germination. We explored the role of DNA damage, repair, cell cycle progression and antioxidant machinery in germination of seeds of Solanum melongena L. Read More

View Article and Full-Text PDF
December 2020

Oxidative DNA Damage Accelerates Skin Inflammation in Pristane-Induced Lupus Model.

Front Immunol 2020 24;11:554725. Epub 2020 Sep 24.

Division of Pediatric Infectious Diseases and Immunology, Cedars-Sinai Medical Center, Los Angeles, CA, United States.

Systemic Lupus Erythematosus (SLE) is a chronic inflammatory autoimmune disease in which type I interferons (IFN) play a key role. The IFN response can be triggered when oxidized DNA engages the cytosolic DNA sensing platform cGAS-STING, but the repair mechanisms that modulate this process and govern disease progression are unclear. To gain insight into this biology, we interrogated the role of oxyguanine glycosylase 1 (OGG1), which repairs oxidized guanine 8-Oxo-2'-deoxyguanosine (8-OH-dG), in the pristane-induced mouse model of SLE. Read More

View Article and Full-Text PDF

The role of AMPK in metabolism and its influence on DNA damage repair.

Mol Biol Rep 2020 Nov 18;47(11):9075-9086. Epub 2020 Oct 18.

DNA Damage Laboratory of Food Science Department, Faculty of Pharmacy, Medical University of Lodz, Ul. Muszynskiego 1, 90-151, Lodz, Poland.

One of the most complex health disproportions in the human body is the metabolic syndrome (MetS). It can result in serious health consequences such as type 2 diabetes mellitus, atherosclerosis or insulin resistance. The center of energy regulation in human is AMP-activated protein kinase (AMPK), which modulates cells' metabolic pathways and protects them against negative effects of metabolic stress, e. Read More

View Article and Full-Text PDF
November 2020

Long non‑coding RNA NONHSAT143692.2 is involved in oxidative DNA damage repair in the lens by regulating the miR‑4728‑5p/OGG1 axis.

Int J Mol Med 2020 Nov 24;46(5):1838-1848. Epub 2020 Aug 24.

Eye Institute, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.

Age‑related cataract (ARC) is the leading cause of blindness worldwide. Oxidative DNA damage is a biochemical feature of ARC pathogenesis. The present study investigated the role of long non‑coding RNAs in the DNA repair of oxidative damage, partially the regulation of the DNA repair gene, 8‑oxoguanine DNA glycosylase (OGG1), in lens affected by ARC. Read More

View Article and Full-Text PDF
November 2020

Modulation of the Apurinic/Apyrimidinic Endonuclease Activity of Human APE1 and of Its Natural Polymorphic Variants by Base Excision Repair Proteins.

Int J Mol Sci 2020 Sep 28;21(19). Epub 2020 Sep 28.

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.

Human apurinic/apyrimidinic endonuclease 1 (APE1) is known to be a critical player of the base excision repair (BER) pathway. In general, BER involves consecutive actions of DNA glycosylases, AP endonucleases, DNA polymerases, and DNA ligases. It is known that these proteins interact with APE1 either at upstream or downstream steps of BER. Read More

View Article and Full-Text PDF
September 2020

DNA repair enzyme OGG1 promotes alveolar progenitor cell renewal and relieves PM2.5-induced lung injury and fibrosis.

Ecotoxicol Environ Saf 2020 Dec 22;205:111283. Epub 2020 Sep 22.

Clinical Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China. Electronic address:

Fine particulate matter (PM2.5) airborne pollution increases the risk of chronic respiratory diseases, such as idiopathic pulmonary fibrosis (IPF), which is characterized by non-specific inflammation of the interstitial lung and extensive deposition of collagen fibers. Type 2 alveolar epithelial cells (AEC2s) are alveolar stem cells in the adult lung that contribute to the lung repair process through complex signaling. Read More

View Article and Full-Text PDF
December 2020

Small-Molecule Inhibitor of 8-Oxoguanine DNA Glycosylase 1 Regulates Inflammatory Responses during Infection.

J Immunol 2020 10 14;205(8):2231-2242. Epub 2020 Sep 14.

Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58203;

The DNA repair enzyme 8-oxoguanine DNA glycosylase 1 (OGG1), which excises 8-oxo-7,8-dihydroguanine lesions induced in DNA by reactive oxygen species, has been linked to the pathogenesis of lung diseases associated with bacterial infections. A recently developed small molecule, SU0268, has demonstrated selective inhibition of OGG1 activity; however, its role in attenuating inflammatory responses has not been tested. In this study, we report that SU0268 has a favorable effect on bacterial infection both in mouse alveolar macrophages (MH-S cells) and in C57BL/6 wild-type mice by suppressing inflammatory responses, particularly promoting type I IFN responses. Read More

View Article and Full-Text PDF
October 2020

MUTYH: Not just polyposis.

World J Clin Oncol 2020 Jul;11(7):428-449

Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Via dei Vestini 66100, Italy.

MUTYH is a base excision repair enzyme, it plays a crucial role in the correction of DNA errors from guanine oxidation and may be considered a cell protective factor. In humans it is an adenine DNA glycosylase that removes adenine misincorporated in 7,8-dihydro-8-oxoguanine (8-oxoG) pairs, inducing G:C to T:A transversions. MUTYH functionally cooperates with OGG1 that eliminates 8-oxodG derived from excessive reactive oxygen species production. Read More

View Article and Full-Text PDF

Chronic exposure to environmentally relevant concentration of fluoride alters Ogg1 and Rad51 expressions in mice: Involvement of epigenetic regulation.

Ecotoxicol Environ Saf 2020 Oct 11;202:110962. Epub 2020 Jul 11.

Department of Zoology, Visva-Bharati, Santiniketan, 731235, West Bengal, India. Electronic address:

Chronic exposure to fluoride (F) beyond the permissible limit (1.5 ppm) is known to cause detrimental health effects by induction of oxidative stress-mediated DNA damage overpowering the DNA repair machinery. In the present study, we assessed F induced oxidative stress through monitoring biochemical parameters and looked into the effect of chronic F exposure on two crucial DNA repair genes Ogg1 and Rad51 having important role against ROS induced DNA damages. Read More

View Article and Full-Text PDF
October 2020

Elucidation of Mechanisms of Topotecan-Induced Cell Death in Human Breast MCF-7 Cancer Cells by Gene Expression Analysis.

Front Genet 2020 17;11:775. Epub 2020 Jul 17.

Biostatistics and Computational Biology Branch, National Institute of Environmental Health Sciences, Durham, NC, United States.

Topotecan is a clinically active anticancer agent for the management of various human tumors. While the principal mechanism of tumor cell killing by topotecan is due to its interactions with topoisomerase I and formation of DNA double-strand breaks, recent studies suggest that mechanisms involving generation of reactive free radicals and induction of oxidative stress may play a significant role in topotecan-dependent tumor cell death. We have shown that topotecan generates a topotecan radical following one-electron oxidation by a peroxidase-hydrogen peroxide system which reacts with reduced glutathione and cysteine, forming the glutathiyl and cysteinyl radicals, respectively. Read More

View Article and Full-Text PDF

Mitochondrial expression of the DNA repair enzyme OGG1 improves the prognosis of pancreatic ductal adenocarcinoma.

Pancreatology 2020 Sep 25;20(6):1175-1182. Epub 2020 Jul 25.

Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, 812-8582, Fukuoka, Japan.

Background/objectives: 8-Hydroxydeoxyguanosine (8-OHdG) is an indicator of oxidative stress and causes transversion mutations and carcinogenesis. 8-OHdG is excision repaired by 8-OHdG DNA glycosylase 1 (OGG1), which is classified as nuclear and mitochondrial subtypes. We aimed to clarify the role of OGG1 in pancreatic ductal adenocarcinoma (PDAC). Read More

View Article and Full-Text PDF
September 2020

Lycopene Protects against Smoking-Induced Lung Cancer by Inducing Base Excision Repair.

Antioxidants (Basel) 2020 Jul 21;9(7). Epub 2020 Jul 21.

Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Room 3204, Kannapolis, NC 28081, USA.

Background: Oxidative stress plays a critical role in lung cancer progression. Carotenoids are efficient antioxidants. The objective of this study was to explore the efficacy of all-trans retinoic acid (ATRA) and carotenoids in cigarette smoke-induced oxidative stress within A549 human lung cancer epithelial cells. Read More

View Article and Full-Text PDF

Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM from Beijing, China, in Winter.

Int J Environ Res Public Health 2020 07 6;17(13). Epub 2020 Jul 6.

College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China.

Epidemiological studies have corroborated that respiratory diseases, including lung cancer, are related to fine particulate matter (<2.5 μm) (PM) exposure. The toxic responses of PM are greatly influenced by the source of PM. Read More

View Article and Full-Text PDF

The impacts of prominent gene polymorphisms in DNA repair enzymes on Parkinson's disease.

Neurosci Lett 2020 09 27;735:135203. Epub 2020 Jun 27.

Gazi University, Faculty of Pharmacy, Toxicology Department, 06330, Ankara, Turkey.

Parkinson's Disease (PD), a chronic and progressive neurodegenerative disease of the brain, is associated with the loss of dopaminergic neurons. Its pathogenesis remains unclear; however, oxidative DNA damage due to reactive oxygen species (ROS) is believed to play a major role in the etiology of PD. DNA repair systems can mitigate oxidative DNA damage and help to maintain genomic stability and thus prevent neuronal death. Read More

View Article and Full-Text PDF
September 2020