66 results match your criteria role metacyclogenesis


The Gene Encodes for an Atypical Dual Specificity Lipid-Like Phosphatase Expressed in Promastigotes and Amastigotes; Substrate Specificity, Intracellular Localizations, and Putative Role(s).

Front Cell Infect Microbiol 2021 25;11:591868. Epub 2021 Mar 25.

Intracellular Parasitism Laboratory, Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece.

The intracellular protozoan parasites of the genus are responsible for Leishmaniases, vector borne diseases with a wide range of clinical manifestations. causes visceral leishmaniasis (kala azar), the most severe of these diseases. Along their biological cycle, parasites undergo distinct developmental transitions including metacyclogenesis and differentiation of metacyclic promastigotes (MPs) to amastigotes. Read More

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Stage-Specific Differential Gene Expression of Glutathione Peroxidase in and .

Rep Biochem Mol Biol 2020 Oct;9(3):324-330

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: and are the etiological agents of cutaneous leishmaniosis. Leishmania species cause a board spectrum of phenotypes. A small number of genes are differentially expressed between them that have likely an important role in the disease phenotype. Read More

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October 2020

Quantitative proteomic analysis reveals differentially expressed proteins in Leishmania major metacyclogenesis.

Microb Pathog 2020 Dec 2;149:104557. Epub 2020 Oct 2.

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Medical Lab Technology, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Iran. Electronic address:

Leishmaniasis is an infectious disease caused by Leishmania that widespread in 98 countries. The differentiation of Leishmania (L) from procyclic to metacyclic promastigote has occurred along with morphological and biochemical changes in proteome scale. We aim here to identify the proteomes of two successive developmental forms (procyclic and metacyclic promastigotes) from Leishmania major isolates using SWATH-MS quantitative proteomics technique. Read More

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December 2020

Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications: Study of changes in epigenetic marks through the parasite's metacyclogenesis and life cycle.

J Proteomics 2020 08 29;225:103847. Epub 2020 May 29.

Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil; Center of Toxins, Immune Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, SP, Brazil. Electronic address:

Trypanosome histone N-terminal sequences are very divergent from the other eukaryotes, although they are still decorated by post-translational modifications (PTMs). Here, we used a highly robust workflow to analyze histone PTMs in the parasite Trypanosoma cruzi using mass spectrometry-based (MS-based) data-independent acquisition (DIA). We adapted the workflow for the analysis of the parasite's histone sequences by modifying the software EpiProfile 2. Read More

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Glutamine Analogues Impair Cell Proliferation, the Intracellular Cycle and Metacyclogenesis in .

Molecules 2020 Apr 2;25(7). Epub 2020 Apr 2.

Laboratory of Biochemistry of Tryps-LaBTryps, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 1374, 05508000 Sao Paulo, Brazil.

is the aetiologic agent of Chagas disease, which affects people in the Americas and worldwide. The parasite has a complex life cycle that alternates among mammalian hosts and insect vectors. During its life cycle, passes through different environments and faces nutrient shortages. Read More

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The Influence of Environmental Cues on the Development of in Triatominae Vector.

Front Cell Infect Microbiol 2020 21;10:27. Epub 2020 Feb 21.

Laboratório de Bioquímica de Tryps (LaBTryps), Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.

, a hemoflagellate parasite, is the etiological agent of Chagas disease that affects about 6-7 million people worldwide, mostly in Latin America. The parasite life cycle is complex and alternates between an invertebrate host-Triatominae vector-and a mammalian host. The parasite adaptation to the several microenvironments through which it transits is critical to success in establishing infection. Read More

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February 2020

Clearing or subverting the enemy: Role of autophagy in protozoan infections.

Life Sci 2020 Apr 20;247:117453. Epub 2020 Feb 20.

Department of Optometry and Vision Science, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.

The protozoan parasites are evolutionarily divergent, unicellular eukaryotic pathogens representing one of the essential sources of parasitic diseases. These parasites significantly affect the economy and cause public health burdens globally. Protozoan parasites share many cellular features and pathways with their respective host cells. Read More

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Induction of autophagy increases the proteolytic activity of reservosomes during metacyclogenesis.

Autophagy 2021 02 4;17(2):439-456. Epub 2020 Feb 4.

Laboratorio de Biología de Trypanosoma Cruzi y la célula hospedadora, Instituto de Histología y Embriología (IHEM-CONICET), Mendoza, Argentina.

Cruzipain, the major cysteine protease of the pathogenic protozoa , is an important virulence factor that plays a key role in the parasite nutrition, differentiation and host cell infection. Cruzipain is synthesized as a zymogen, matured, and delivered to reservosomes. These organelles that store proteins and lipids ingested by endocytosis undergo a dramatic decrease in number during the metacyclogenesis of . Read More

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February 2021

Single-cell RNA sequencing of from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens.

Proc Natl Acad Sci U S A 2020 02 21;117(5):2613-2621. Epub 2020 Jan 21.

Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University, New Haven, CT 06520;

Tsetse-transmitted African trypanosomes must develop into mammalian-infectious metacyclic cells in the fly's salivary glands (SGs) before transmission to a new host. The molecular mechanisms that underlie this developmental process, known as metacyclogenesis, are poorly understood. Blocking the few metacyclic parasites deposited in saliva from further development in the mammal could prevent disease. Read More

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February 2020

In-depth comparison of cell-based methodological approaches to determine drug susceptibility of visceral Leishmania isolates.

PLoS Negl Trop Dis 2019 12 2;13(12):e0007885. Epub 2019 Dec 2.

Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Antwerp, Belgium.

Monitoring the drug susceptibility of Leishmania isolates still largely relies on standard in vitro cell-based susceptibility assays using (patient-isolated) promastigotes for infection. Although this assay is widely used, no fully standardized/harmonized protocol is yet available hence resulting in the application of a wide variety of host cells (primary cells and cell lines), different drug exposure times, detection methods and endpoint criteria. Advocacy for standardization to decrease inter-laboratory variation and improve interpretation of results has already repeatedly been made, unfortunately still with unsatisfactory progress. Read More

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December 2019

A quantitative proteomic and bioinformatics analysis of proteins in metacyclogenesis of Leishmania tropica.

Acta Trop 2020 Feb 21;202:105227. Epub 2019 Oct 21.

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Recently there has a growing interest in MS-based analysis on Leishmania for biology study, host-parasite interaction and drug target discovery. The aims of this study were to analyzed protein profiles in the procyclic and metacyclic stages of L. tropica, and investigate their potential role in metacyclogenesis molecular mechanisms. Read More

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February 2020

Ascorbate-Dependent Peroxidase (APX) from Leishmania amazonensis Is a Reactive Oxygen Species-Induced Essential Enzyme That Regulates Virulence.

Infect Immun 2019 12 18;87(12). Epub 2019 Nov 18.

Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, USA

The molecular mechanisms underlying biological differences between two species that cause cutaneous disease, and , are poorly understood. In , reactive oxygen species (ROS) signaling drives differentiation of nonvirulent promastigotes into forms capable of infecting host macrophages. Tight spatial and temporal regulation of HO is key to this signaling mechanism, suggesting a role for ascorbate-dependent peroxidase (APX), which degrades mitochondrial HO Earlier studies showed that -null parasites are viable, accumulate higher levels of HO, generate a greater yield of infective metacyclic promastigotes, and have increased virulence. Read More

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December 2019

Bioactive lipids regulate Trypanosoma cruzi development.

Parasitol Res 2019 Sep 2;118(9):2609-2619. Epub 2019 Jul 2.

Instituto de Bioquímica Médica Leopoldo de Meis, Programa de Biologia Molecular e Biotecnologia, Universidade Federal do Rio de Janeiro, CCS, UFRJ, Rio de Janeiro, RJ, 21940-590, Brazil.

Trypanosoma cruzi is the etiological agent of Chagas disease. These parasites undergo dramatic morphological and physiological changes during their life cycle. The human-infective metacyclic trypomastigotes differentiate from epimastigotes inside the midgut of the Triatominae insect vector. Read More

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September 2019

Autophagy: A necessary process during the life-cycle.

Virulence 2019 12 29;10(1):460-469. Epub 2018 Nov 29.

a Laboratorio de Biología de Trypanosoma cruzi y la célula hospedadora, Instituto de Histología y Embriología (IHEM) "Dr. Mario H. Burgos" CONICET , Universidad Nacional de Cuyo , Mendoza , Argentina.

Autophagy is a well-conserved process of self-digestion of intracellular components. is a protozoan parasite with a complex life-cycle that involves insect vectors and mammalian hosts. Like other eukaryotic organisms, possesses an autophagic pathway that is activated during metacyclogenesis, the process that generates the infective forms of parasites. Read More

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December 2019

Lipase Precursor-Like Protein Promotes Miltefosine Tolerance in Leishmania donovani by Enhancing Parasite Infectivity and Eliciting Anti-inflammatory Responses in Host Macrophages.

Antimicrob Agents Chemother 2018 12 26;62(12). Epub 2018 Nov 26.

National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India

The oral drug miltefosine (MIL) was introduced in the Indian subcontinent in the year 2002 for the treatment of visceral leishmaniasis (VL). However, recent reports on its declining efficacy and increasing relapse rates pose a serious concern. An understanding of the factors contributing to MIL tolerance in parasites is critical. Read More

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December 2018

The Potential Role of Humans in the Transmission Cycle of Leishmania major (Kinetoplastida: Trypanosomatidae), the Causative Agent of the Old World Zoonotic Cutaneous Leishmaniasis.

J Med Entomol 2018 Oct;55(6):1588-1593

Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major (Yakimoff and Schokhor, 1914) is known as a neglected tropical disease, transmitted by Phlebotomus papatasi (Scopoli, 1786) in North Africa and the Middle East. The main reservoirs of ZCL are desert gerbils and the role of humans as a reservoir host of the disease is not clearly defined and is therefore investigated in this study. In order to achieve this objective, Ph. Read More

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October 2018

Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery.

Mem Inst Oswaldo Cruz 2018 18;113(6):e170531. Epub 2018 Jun 18.

Instituto Carlos Chagas, Fundação Oswaldo Cruz-Fiocruz, Curitiba, PR, Brasil.

BACKGROUND Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. Read More

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Translational repression by an RNA-binding protein promotes differentiation to infective forms in Trypanosoma cruzi.

PLoS Pathog 2018 06 4;14(6):e1007059. Epub 2018 Jun 4.

Instituto de Investigaciones Biotecnológicas, UNSAM-CONICET, San Martín, Provincia de Buenos Aires, Argentina.

Trypanosomes, protozoan parasites of medical importance, essentially rely on post-transcriptional mechanisms to regulate gene expression in insect vectors and vertebrate hosts. RNA binding proteins (RBPs) that associate to the 3'-UTR of mature mRNAs are thought to orchestrate master developmental programs for these processes to happen. Yet, the molecular mechanisms by which differentiation occurs remain largely unexplored in these human pathogens. Read More

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Knockout of the CCCH zinc finger protein TcZC3H31 blocks Trypanosoma cruzi differentiation into the infective metacyclic form.

Mol Biochem Parasitol 2018 04 2;221:1-9. Epub 2018 Feb 2.

Instituto Carlos Chagas/Fiocruz-PR, Rua Prof. Algacyr Munhoz Mader, 3775-CIC, Curitiba, PR, 81350-010, Brazil. Electronic address:

In the protozoan parasite Trypanosoma cruzi - the causative agent of Chagas disease - gene expression control is mainly post-transcriptional, where RNA-binding proteins (RBPs) play a central role, by controlling mRNA stability, distribution and translation. A large variety of RBPs are encoded in the T. cruzi genome, including the CCCH-type zinc finger (CCCH ZnF) protein family, which is characterized by the presence of the C-X-C-X-C-X-H (CCCH) motif. Read More

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Arginase expression modulates nitric oxide production in Leishmania (Leishmania) amazonensis.

PLoS One 2017 14;12(11):e0187186. Epub 2017 Nov 14.

Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil.

Background: Arginase is an enzyme that converts L-arginine to urea and L-ornithine, an essential substrate for the polyamine pathway supporting Leishmania (Leishmania) amazonensis replication and its survival in the mammalian host. L-arginine is also the substrate of macrophage nitric oxide synthase 2 (NOS2) to produce nitric oxide (NO) that kills the parasite. This competition can define the fate of Leishmania infection. Read More

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December 2017

Leishmania LABCG1 and LABCG2 transporters are involved in virulence and oxidative stress: functional linkage with autophagy.

Parasit Vectors 2017 May 30;10(1):267. Epub 2017 May 30.

Instituto de Parasitología y Biomedicina "López-Neyra", IPBLN-CSIC, Parque Tecnológico de Ciencias de la Salud, Avda. del Conocimiento s/n, 18016, Granada, Spain.

Background: The G subfamily of ABC (ATP-binding cassette) transporters of Leishmania include 6 genes (ABCG1-G6), some with relevant biological functions associated with drug resistance and phospholipid transport. Several studies have shown that Leishmania LABCG2 transporter plays a role in the exposure of phosphatidylserine (PS), in virulence and in resistance to antimonials. However, the involvement of this transporter in other key biological processes has not been studied. Read More

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Different Roles of Mitochondrial Calcium Uniporter Complex Subunits in Growth and Infectivity of .

mBio 2017 05 9;8(3). Epub 2017 May 9.

Departamento de Patología Clínica, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil

is the agent of Chagas disease, and the finding that this parasite possesses a mitochondrial calcium uniporter (TcMCU) with characteristics similar to that of mammalian mitochondria was fundamental for the discovery of the molecular nature of MCU in eukaryotes. We report here that ablation of , or its paralog , by clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 led to a marked decrease in mitochondrial Ca uptake without affecting the membrane potential of these cells, whereas overexpression of each gene caused a significant increase in the ability of mitochondria to accumulate Ca While knockout (KO) epimastigotes were viable and able to differentiate into trypomastigotes, infect host cells, and replicate normally, ablation of resulted in epimastigotes having an important growth defect, lower rates of respiration and metacyclogenesis, more pronounced autophagy changes under starvation, and significantly reduced infectivity. Overexpression of , in contrast to what was proposed for its mammalian ortholog, did not result in a dominant negative effect on TcMCU. Read More

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Leishmania HASP and SHERP Genes Are Required for In Vivo Differentiation, Parasite Transmission and Virulence Attenuation in the Host.

PLoS Pathog 2017 01 17;13(1):e1006130. Epub 2017 Jan 17.

Centre for Immunology and Infection, Department of Biology, University of York, York, United Kingdom.

Differentiation of extracellular Leishmania promastigotes within their sand fly vector, termed metacyclogenesis, is considered to be essential for parasites to regain mammalian host infectivity. Metacyclogenesis is accompanied by changes in the local parasite environment, including secretion of complex glycoconjugates within the promastigote secretory gel and colonization and degradation of the sand fly stomodeal valve. Deletion of the stage-regulated HASP and SHERP genes on chromosome 23 of Leishmania major is known to stall metacyclogenesis in the sand fly but not in in vitro culture. Read More

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January 2017

Comparative effects of histone deacetylases inhibitors and resveratrol on Trypanosoma cruzi replication, differentiation, infectivity and gene expression.

Authors:
Vanina A Campo

Int J Parasitol Drugs Drug Resist 2017 04 21;7(1):23-33. Epub 2016 Dec 21.

Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo Ugalde" (IIB-INTECH), Universidad Nacional San Martín (UNSAM), Av. 25 de Mayo y Francia, Campus Miguelete, CP1650, San Martín, Provincia de Buenos Aires, Argentina. Electronic address:

Histone post-translational modification, mediated by histone acetyltransferases and deacetylases, is one of the most studied factors affecting gene expression. Recent data showing differential histone acetylation states during the Trypanosoma cruzi cell cycle suggest a role for epigenetics in the control of this process. As a starting point to study the role of histone deacetylases in the control of gene expression and the consequences of their inhibition and activation in the biology of T. Read More

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A GCN2-Like eIF2α Kinase (LdeK1) of Leishmania donovani and Its Possible Role in Stress Response.

PLoS One 2016 1;11(6):e0156032. Epub 2016 Jun 1.

Cell and Molecular Biology Laboratory, Department of Biotechnology, Savitribai Phule Pune University, Ganeshkhind, Pune-411007, India.

Translation regulation in Leishmania parasites assumes significance particularly because they encounter myriad of stresses during their life cycle. The eukaryotic initiation factor 2α (eIF2α) kinases, the well-known regulators of translation initiation in higher eukaryotes have now been found to control various processes in these protozoan parasites as well. Here, we report on cloning and characterization of a GCN2-like eIF2α kinase from L. Read More

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Dynamics of sterol synthesis during development of Leishmania spp. parasites to their virulent form.

Parasit Vectors 2016 Apr 12;9:200. Epub 2016 Apr 12.

Departments of Internal Medicine, Microbiology and Epidemiology, University of Iowa, Iowa City, IA, USA.

Background: The Leishmania spp. protozoa, the causative agents of the "neglected" tropical disease leishmaniasis, are transmitted to mammals by sand fly vectors. Within the sand fly, parasites transform from amastigotes to procyclic promastigotes, followed by development of virulent (metacyclic) promastigote forms. Read More

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Metabolomics-Based Study of Logarithmic and Stationary Phases of Promastigotes in Leishmania major by 1H NMR Spectroscopy.

Iran Biomed J 2016 23;20(2):77-83. Epub 2015 Nov 23.

Dept. of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.

Background: Cutaneous leishmaniasis is one of the most important parasitic diseases in humans. In this disease, one of the responsible organisms is Leishmania major, which is transmitted by sandfly vector. There are specific differences in biochemical profiles and metabolite pathways in logarithmic and stationary phases of Leishmania parasites. Read More

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October 2016

Kinetoplastid Membrane Protein-11 as a Vaccine Candidate and a Virulence Factor in Leishmania.

Front Immunol 2015 13;6:524. Epub 2015 Oct 13.

Laboratório de Tecnologia Imunológica, Instituto de Tecnologia em Imunobiológicos, Fundação Oswaldo Cruz , Rio de Janeiro , Brazil.

Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid protozoa, is considered a potential candidate for a leishmaniasis vaccine. In Leishmania amazonensis, KMP-11 is expressed in promastigotes and amastigotes. In both stages, the protein was found in association with membrane structures at the cell surface, flagellar pocket, and intracellular vesicles. Read More

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November 2015

Functional studies of TcRjl, a novel GTPase of Trypanosoma cruzi, reveals phenotypes related with MAPK activation during parasite differentiation and after heterologous expression in Drosophila model system.

Biochem Biophys Res Commun 2015 Nov 25;467(1):115-20. Epub 2015 Sep 25.

Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, CCS, UFRJ, Rio de Janeiro, Brazil; Instituto Nacional de Ciência e Tecnologia-Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INCT-IMPeTAM), Brazil. Electronic address:

The life cycle of the protozoan parasite Trypanosoma cruzi comprises rounds of proliferative cycles and differentiation in distinct host environments. Ras GTPases are molecular switches that play pivotal regulatory functions in cell fate. Rjl is a novel GTPase with unknown function. Read More

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November 2015

A dominant negative form of inositol 1,4,5-trisphosphate receptor induces metacyclogenesis and increases mitochondrial density in Trypanosoma cruzi.

Biochem Biophys Res Commun 2015 Oct 12;466(3):475-80. Epub 2015 Sep 12.

Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute, Saitama, 351-0198, Japan; Calcium Oscillation Project, International Cooperative Research Project and Solution-Oriented Research for Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012, Japan. Electronic address:

Inositol 1,4,5-trisphosphate receptor (IP3R) is a key regulator of intracellular Ca(2+) concentration that release Ca(2+) from Ca(2+) stores in response to various external stimuli. IP3R also works as a signal hub which form a platform for interacting with various proteins involved in diverse cell signaling. Previously, we have identified an IP3R homolog in the parasitic protist, Trypanosoma cruzi (TcIP3R). Read More

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October 2015