1,102,938 results match your criteria role cells


Isoflurane induces Art2-Rsp5-dependent endocytosis of Bap2 in yeast.

FEBS Open Bio 2021 Sep 18. Epub 2021 Sep 18.

Center of Frontier Oral Science, Osaka University, Graduate School of Dentistry.

Although general anesthesia is indispensable during modern surgical procedures, the mechanism by which inhalation anesthetics act on the synaptic membrane at the molecular and cellular level is largely unknown. In this study, we used yeast cells to examine the effect of isoflurane, an inhalation anesthetic, on membrane proteins. Bap2, an amino acid transporter localized on the plasma membrane, was endocytosed when yeast cells were treated with isoflurane. Read More

View Article and Full-Text PDF
September 2021

ASXL1 promotes adrenocortical carcinoma and is associated with chemoresistance to EDP regimen.

Aging (Albany NY) 2021 Sep 18;13(undefined). Epub 2021 Sep 18.

Department of Urology, Fourth Affiliated Hospital of China Medical University, Shenyang 100032, Liaoning Province, P.R. China.

Adrenocortical carcinoma (ACC) is a rare but aggressive disease that lacks definitive treatment. We aim to evaluate role of ASXL1 in ACC and exploit its therapeutic merits therein. We performed reproduction of datasets of the Cancer Genome Atlas (TCGA), GDSC (Genomics of Drug Sensitivity in Cancer) and Human Protein Atlas using platforms of cBioPortal, UALCAN, NET-GE, GSEA and GEPIA. Read More

View Article and Full-Text PDF
September 2021

Involvement of obestatin, cyclin-dependent kinase and protein kinase C in control of feline ovarian cell viability and hormones release.

Reprod Biol 2021 Sep 15;21(4):100560. Epub 2021 Sep 15.

Department of Zoology and Anthropology, Constantine the Philosopher University, Nitra Slovakia.

The aim of our in vitro study was to understand the role of obestatin, cyclin-dependent kinase (CDK) and protein kinase C (PKC) in the control of basic feline ovarian cell functions (viability, ovarian hormones release), as well as the role of protein kinases in mediating the effect of obestatin on these processes. For this purpose, we analyzed the effect of obestatin (0, 10 and 100 ng/mL) alone or in combination with CDK blocker olomoucine (100 ng/mL) or PKC blocker calphostin-c (100 ng/mL) on cultured feline ovarian fragments or granulosa cells. The release of progesterone (P4), testosterone (T) and estradiol (E2) by isolated ovarian follicular fragments were evaluated by ELISA. Read More

View Article and Full-Text PDF
September 2021

Plants are a natural source of nitrous oxide even in field conditions as explained by N site preference.

Sci Total Environ 2021 Sep 11;805:150262. Epub 2021 Sep 11.

Key Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Plants are either recognized to produce nitrous oxide (NO) or considered as a medium to transport soil-produced NO. To date, it is not clear whether in their habitat plants conduit NO produced in soil or are a natural source. We aimed to understand role of plants in NO emissions in field conditions. Read More

View Article and Full-Text PDF
September 2021

Upregulation of UGT1A1 expression by ursolic acid and oleanolic acid via the inhibition of the PKC/NF-κB signaling pathway.

Phytomedicine 2021 Aug 25;92:153726. Epub 2021 Aug 25.

Clinical Pharmacology Institute, Nanchang University, Nanchang 330006, PR China. Electronic address:

Background: Isomeric ursolic acid (UA) and oleanolic acid (OA) compounds have recently garnered great attention due to their biological effects. Previously, it had been shown that UA and OA can exert important pharmacological action via the protein kinase C (PKC) and nuclear factor-κB (NF-κB) signaling, and that they can induce the expression of UDP-glucuronosyltransferase 1A1 (UGT1A1) in HepG2 cells. This study aims to investigate the role of PKC/NF-κB signaling in regulating the expression of UGT1A1 and examine how UA and OA induce UGT1A1 based on this signaling pathway. Read More

View Article and Full-Text PDF

Platelet-rich plasma attenuates interleukin-1β-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2α.

Tissue Cell 2021 Sep 8;73:101646. Epub 2021 Sep 8.

Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, 95 Yong an Road, Xicheng District, Beijing, 100050, China. Electronic address:

Osteoarthritis (OA) is a prevailing chronic disease in Orthopedics that characterized with severely damaged cartilage and subchondral bone, thus leading to profound disorders of synovial joints. Platelet-rich plasma (PRP) has been applied as a popular non-operative treatment option for promoting musculoskeletal healing. Our previous work demonstrated that PRP protected chondrocytes from interleukin-1β (IL-1β)-induced apoptosis in vitro. Read More

View Article and Full-Text PDF
September 2021

Reactive oxygen species (ROS) in cancer pathogenesis and therapy: An update on the role of ROS in anticancer action of benzophenanthridine alkaloids.

Biomed Pharmacother 2021 Sep 15;143:112142. Epub 2021 Sep 15.

Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, Qatar; Dermatology Institute, Academic Health System, Hamad Medical Corporation, Doha 3050, Qatar; Department of Dermatology and Venereology, Rumailah Hospital, Hamad Medical Corporation, Doha 3050, Qatar; Laboratory Animal Research Center, Qatar University, Doha 2713, Qatar. Electronic address:

Reactive oxygen species play crucial role in biological homeostasis and pathogenesis of human diseases including cancer. In this line, now it has become evident that ROS level/concentration is a major factor in the growth, progression and stemness of cancer cells. Moreover, cancer cells maintain a delicate balance between ROS and antioxidants to promote pathogenesis and clinical challenges via targeting a battery of signaling pathways converging to cancer hallmarks. Read More

View Article and Full-Text PDF
September 2021

Role of vitamin D/VDR nuclear translocation in down-regulation of NF-κB/NLRP3/caspase-1 axis in lupus nephritis.

Int Immunopharmacol 2021 Sep 15;100:108131. Epub 2021 Sep 15.

The Second Affiliated Hospital of Xi'an Jiaotong University (Xibei Hospital), Xi'an 710004, Shaanxi, China. Electronic address:

Background: Vitamin D receptor (VDR) and NLRP3 inflammasome play critical roles in lupus nephritis (LN) pathogenesis.

Aim Of The Study: This study explored the therapeutic effect of VDR agonist on LN and its molecular mechanism to inhibit NLRP3 signalling.

Methods: C57BL/6 mice, lupus-prone MRL/lpr mice, and VDR agonist paricacitol-treated MRL/lpr mice (300 ng/kg/mouse per dose, 5 times/week for 8 weeks from 8 weeks old) were used to assess kidney histopathology and measure proteinuria, serum anti-ds-DNA antibody and expression of NF-κB/NLRP3/caspase-1/IL-1β/IL-18 axis. Read More

View Article and Full-Text PDF
September 2021

The role of transforming growth factor β in upper gastrointestinal cancers: A systematic review.

Cancer Treat Rev 2021 Sep 2;100:102285. Epub 2021 Sep 2.

Department of Medical Oncology, Cancer Center Amsterdam, Amsterdam University Medical Centers, University of Amsterdam, De Boelelaan 1117-1118, 1081 HV Amsterdam, The Netherlands.

Esophageal and gastric malignancies are associated with poor prognosis, in part due to development of recurrences or metastases after curative treatment. The transforming growth factor β (TGF-β) pathway might play a role in the development of treatment resistance. In this systematic review, we provide an overview of preclinical studies investigating the role of TGF-β in esophageal and gastric malignancies. Read More

View Article and Full-Text PDF
September 2021

Exosome-mediated circ_0001846 participates in IL-1β-induced chondrocyte cell damage by miR-149-5p-dependent regulation of WNT5B.

Clin Immunol 2021 Sep 15:108856. Epub 2021 Sep 15.

Trauma Center, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu, China. Electronic address:

Aims: Osteoarthritis (OA) is the leading cause of physical disability in middle-aged and elderly people globally. Previous studies have revealed that circular RNA (circRNA) is involved in the pathogenesis of OA. In this study, we studied the role of circ_0001846 in interleukin-1β (IL-1β)-induced OA progression. Read More

View Article and Full-Text PDF
September 2021

Innovative Therapies for Neuroblastoma: The Surprisingly Potent Role of Iron Chelation in Up-Regulating Metastasis and Tumor Suppressors and Down-Regulating the Key Oncogene, N-myc.

Pharmacol Res 2021 Sep 15:105889. Epub 2021 Sep 15.

Centre for Cancer Cell Biology and Drug Discovery, Griffith Institute for Drug Discovery, Griffith University, Nathan, Brisbane, Queensland, 4111, Australia; Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan. Electronic address:

Iron is an indispensable requirement for essential biological processes in cancer cells. Due to the greater proliferation of neoplastic cells, their demand for iron is considerably higher relative to normal cells, making them highly susceptible to iron depletion. Understanding this sensitive relationship led to research exploring the effect of iron chelation therapy for cancer treatment. Read More

View Article and Full-Text PDF
September 2021

LncRNA SOCS2-AS1 promotes the progression of glioma via regulating ITGB1 expression.

Neurosci Lett 2021 Sep 15:136248. Epub 2021 Sep 15.

Department of Neurosurgery, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, China.

Background: Accumulating evidence has underscored the important role of long non-coding RNAs (lncRNAs) in the development and progression of glioma. However, the role of lncRNA SOCS2-AS1 in glioma is largely unknown.

Methods: lncRNA SOCS2-AS1 silencing was achieved by specific siRNAs. Read More

View Article and Full-Text PDF
September 2021

Intermittent hypoxia aggravates non-alcoholic fatty liver disease via RIPK3-dependent necroptosis-modulated Nrf2/NFκB signaling pathway.

Life Sci 2021 Sep 15:119963. Epub 2021 Sep 15.

Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China. Electronic address:

Aims: Hepatocyte necroptosis is a critical event in the progression of non-alcoholic fatty liver disease (NAFLD). Obstructive sleep apnea hypopnea syndrome (OSAHS) and chronic intermittent hypoxia (CIH) may be linked with the pathogenesis and the severity of NAFLD. However, the potential role of necroptosis in OSAHS-associated NAFLD has not been evaluated. Read More

View Article and Full-Text PDF
September 2021

Gut dysbiosis, defective autophagy and altered immune responses in neurodegenerative diseases: Tales of a vicious cycle.

Pharmacol Ther 2021 Sep 15:107988. Epub 2021 Sep 15.

NIHR Nottingham Biomedical Research Centre, University of Nottingham, Nottingham NG7 2UH, UK; Nottingham Digestive Diseases Centre, School of Medicine, University of Nottingham, Nottingham NG7 2UH, UK. Electronic address:

The human microbiota comprises trillions of symbiotic microorganisms and is involved in regulating gastrointestinal (GI), immune, nervous system and metabolic homeostasis. Recent observations suggest a bidirectional communication between the gut microbiota and the brain via immune, circulatory and neural pathways, termed the Gut-Brain Axis (GBA). Alterations in gut microbiota composition, such as seen with an increased number of pathobionts and a decreased number of symbionts, termed gut dysbiosis or microbial intestinal dysbiosis, plays a prominent role in the pathogenesis of central nervous system (CNS)-related disorders. Read More

View Article and Full-Text PDF
September 2021

GPR120 inhibits colitis through regulation of CD4T cell IL-10 production.

Gastroenterology 2021 Sep 15. Epub 2021 Sep 15.

Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555, USA; Department of Pathology, The University of Texas Medical Branch, Galveston, TX, 77555, USA. Electronic address:

Background & Aims: G protein-coupled receptor (GPR) 120 has been implicated in regulating metabolic syndromes with anti-inflammatory function. However, the role of GPR120 in intestinal inflammation is unknown. Here, we investigated whether and how GPR120 regulates CD4T cell function to inhibit colitis development. Read More

View Article and Full-Text PDF
September 2021

Hepatic stellate cell senescence in liver fibrosis: Characteristics, mechanisms and perspectives.

Mech Ageing Dev 2021 Sep 16;199:111572. Epub 2021 Sep 16.

Dept. of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands. Electronic address:

Myofibroblasts play an important role in fibrogenesis. Hepatic stellate cells are the main precursors of myofibroblasts. Cellular senescence is the terminal cell fate in which proliferating cells undergo irreversible cell cycle arrest. Read More

View Article and Full-Text PDF
September 2021

Overexpression of miR-132-3p contributes to neuronal protection in in vitro and in vivo models of Alzheimer's disease.

Behav Brain Res 2021 Sep 15:113584. Epub 2021 Sep 15.

Central Laboratory, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde Foshan), Foshan, Guangdong, 528300, China; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China. Electronic address:

One of the neuropathological hallmarks of Alzheimer's disease (AD) is accumulation and deposition of amyloid-beta (Aβ) plaques in the hippocampus. Recently, microRNAs (miRNAs), have been demonstrated to play an essential role in AD. We have previously demonstrated that miR-132-3p exerts neuroprotection via regulating histone deacetylase 3 (HDAC3) in a mouse model of AD. Read More

View Article and Full-Text PDF
September 2021

Fluoride regulates chondrocyte proliferation and autophagy via PI3K/AKT/mTOR signaling pathway.

Chem Biol Interact 2021 Sep 15:109659. Epub 2021 Sep 15.

Department of Environmental Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, PR China. Electronic address:

Fluorine is an essential trace element for human health. However, excessive fluoride intake causes skeletal fluorosis which affects cartilage development. Fluoride inhibited chondrocyte proliferation which is the initial and critical step of endochondral ossification, but the underlying mechanism has not been clearly illustrated. Read More

View Article and Full-Text PDF
September 2021

Serine/threonine kinase 17b (STK17B) signaling regulates Purkinje cell dendritic development and is altered in multiple spinocerebellar ataxias.

Eur J Neurosci 2021 Sep 18. Epub 2021 Sep 18.

Institute of Anatomy, Department of Biomedicine, University of Basel, Basel, Switzerland.

Serine/threonine kinase 17b (STK17B, also known as DRAK2) is known to be a downstream effector of Protein Kinase C (PKC) in the immune system, in particular T-lymphocytes. PKC activity also plays a critical role for dendritic development and synaptic maturation and plasticity in cerebellar Purkinje cells. We present evidence that STK17B is strongly expressed in mouse cerebellar Purkinje cells starting in the early postnatal period and remaining highly expressed throughout adult stages and that STK17B is a target of PKC phosphorylation in the cerebellum. Read More

View Article and Full-Text PDF
September 2021

Macrophages in the cochlea; an immunological link between risk factors and progressive hearing loss.

Glia 2021 Sep 18. Epub 2021 Sep 18.

Clinical and Experimental Sciences, Faculty of Medicine, IfLS, University of Southampton, Southampton, UK.

Macrophages are abundant in the cochlea; however, their role in hearing loss is not well understood. Insults to the cochlea, such as noise or insertion of a cochlear implant, cause an inflammatory response, which includes activation of tissue-resident macrophages. Activation is characterized by changes in macrophage morphology, mediator expression, and distribution. Read More

View Article and Full-Text PDF
September 2021

Secretion of a low-molecular-weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells.

Traffic 2021 Sep 18. Epub 2021 Sep 18.

Department of Biotechnology, Graduate School of Engineering.

GRP94 (glucose-regulated protein 94) is a well-studied chaperone with a KDEL (lysine, aspartic acid, glutamic acid, and leucine) motif at its C-terminal, which is responsible for GRP94 localization in the endoplasmic reticulum (ER). GRP94 is upregulated during ER stress to help fold unfolded proteins or direct proteins to ER-associated degradation. In a previous study, engineered GRP94 without the KDEL motif stimulated a powerful immune response in vaccine cells. Read More

View Article and Full-Text PDF
September 2021

The mutagenic effect of tobacco smoke on male fertility.

Environ Sci Pollut Res Int 2021 Sep 18. Epub 2021 Sep 18.

College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates.

Despite the association between tobacco use and the harmful effects on general health as well as male fertility parameters, smoking remains globally prevalent. The main content of tobacco smoke is nicotine and its metabolite cotinine. These compounds can pass the blood-testis barrier, which subsequently causes harm of diverse degree to the germ cells. Read More

View Article and Full-Text PDF
September 2021

Circ_0007534 Silencing Inhibits the Proliferation, Migration and Invasion and Induces the Apoptosis of Glioma Cells Partly Through Down-Regulating PROX1 Via Elevating miR-22-3p Level.

Cell Mol Neurobiol 2021 Sep 18. Epub 2021 Sep 18.

Department of Neurosurgery, The Second Affiliated Hospital of Shenzhen University (People's Hospital of Shenzhen Baoan District), No. 118, Longjing Second Road, Xin'an Street, Baoan District, Shenzhen, 518101, China.

Glioma is a common malignant brain neoplasm. The role and mechanism of circular RNA 0,007,534 (circ_0007534) in glioma progression were investigated in this study. The expression of circ_0007534, microRNA-22-3p (miR-22-3p) and prospero homeobox protein 1 (PROX1) messenger RNA (mRNA) was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Read More

View Article and Full-Text PDF
September 2021

Role of Inflammatory Cytokines, Growth Factors and Adipokines in Adipogenesis and Insulin Resistance.

Inflammation 2021 Sep 18. Epub 2021 Sep 18.

Biomedical Research Center, Qatar University, Doha, Qatar.

Obesity, manifested by increased adiposity, represents a main cause of morbidity in the developed countries, causing increased risk of insulin resistance and type 2 diabetes mellitus. Recruitment of macrophages and activation of innate immunity represent the initial insult, which can be further exacerbated through secretion of chemokines and adipocytokines from activated macrophages and other cells within the adipose tissue. These events can impact adipogenesis, causing dysfunction of the adipose tissue and increased risk of insulin resistance. Read More

View Article and Full-Text PDF
September 2021

Casein Kinase-1-Alpha Inhibitor (D4476) Sensitizes Microsatellite Instable Colorectal Cancer Cells to 5-Fluorouracil via Authophagy Flux Inhibition.

Arch Immunol Ther Exp (Warsz) 2021 Sep 18;69(1):26. Epub 2021 Sep 18.

Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Adjuvant chemotherapy with 5-fluorouracil (5-FU) does not improve survival of patients suffering from a form of colorectal cancer (CRC) characterized by high level of microsatellite instability (MSI-H). Given the importance of autophagy and multi-drug-resistant (MDR) proteins in chemotherapy resistance, as well as the role of casein kinase 1-alpha (CK1α) in the regulation of autophagy, we tested the combined effect of 5-FU and CK1α inhibitor (D4476) on HCT116 cells as a model of MSI-H colorectal cancer. To achieve this goal, the gene expression of Beclin1 and MDR genes, ABCG2 and ABCC3 were analyzed using quantitative real-time polymerase chain reaction. Read More

View Article and Full-Text PDF
September 2021

Stem cell-derived exosomes: A supernova in cosmetic dermatology.

J Cosmet Dermatol 2021 Sep 18. Epub 2021 Sep 18.

Department of Medical Cosmetology, Hengyang Medical School, The First Affiliated Hospital, University of South China, Hengyang, China.

Introduction: Stem cell-derived exosomes are cell-free vesicles secreted by stem cells. Exosomes play a pivotal role in cell-to cell communication due to the functional proteins and genetic information which they carry. In addition, studies on cell migration, tumor invasion, tissue regeneration, myocardial repair after injury, and fracture healing have been widely reported. Read More

View Article and Full-Text PDF
September 2021

Brain regionalization by Polycomb-group proteins and chromatin accessibility.

Bioessays 2021 Sep 18:e2100155. Epub 2021 Sep 18.

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.

During brain development, neural precursor cells (NPCs) in different brain regions produce different types of neurons, and each of these regions plays a different role in the adult brain. Therefore, precise regionalization is essential in the early stages of brain development, and irregular regionalization has been proposed as the cause of neurodevelopmental disorders. The mechanisms underlying brain regionalization have been well studied in terms of morphogen-induced expression of critical transcription factors for regionalization. Read More

View Article and Full-Text PDF
September 2021

Immune signatures of bone marrow cells can independently predict prognosis in patients with myelodysplastic syndrome.

Br J Haematol 2021 Sep 18. Epub 2021 Sep 18.

Division of Haematology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.

Increasing evidence supports the role of the immune microenvironment and associated signalling in the pathogenesis of myelodysplastic syndromes (MDS). Nevertheless, the clinical relevancy of immune signals in patients with MDS remains elusive. To address this, we used single-sample gene-set enrichment analysis to score immune signatures of bone marrow (BM) samples from 176 patients with primary MDS. Read More

View Article and Full-Text PDF
September 2021

HSC70 as a sensor of low temperature: Role in cold-triggered autoinflammatory disorders.

FEBS J 2021 Sep 18. Epub 2021 Sep 18.

CSIR-Centre for Cellular and Molecular Biology, Hyderabad, 500007, India.

Familial cold auto-inflammatory syndrome (FCAS) is a subset of heritable auto-inflammatory disorders wherein inflammatory symptoms aggravate upon exposure of the individual to subnormal temperature. In the past two decades, several mutations in various genes like NLRP3, NLRP12, PLCG2 and NLRC4 have been identified that cause cold-triggered inflammation. However, our understanding about the mechanisms by which cells perceive subnormal temperature, and what keeps the inflammation under check until exposure to low-temperature, is very limited. Read More

View Article and Full-Text PDF
September 2021

Development and validation of a PBRM1-associated immune prognostic model for clear cell renal cell carcinoma.

Cancer Med 2021 Sep 18. Epub 2021 Sep 18.

Department of Anesthesiology, Institute of Anesthesia and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Alteration in the polybromo-1 (PBRM1) protein encoding gene PBRM1 is the second most frequent mutation in clear cell renal cell carcinoma (ccRCC). It causes a series of changes in the tumorigenesis, progression, prognosis, and immune response of ccRCC patients. This study explored the PBRM1-associated immunological features and identified the immune-related genes (IRGs) linked to PBRM1 mutation using bioinformatics methods. Read More

View Article and Full-Text PDF
September 2021