Am J Surg Pathol 2021 Feb 22. Epub 2021 Feb 22.
*Department of Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania, Philadelphia, PA Departments of †Otolaryngology-Head and Neck Surgery ¶Biostatistics **Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN ‡Department of Epidemiology, College of Public Health, University of Kentucky, Lexington, KY §Department of Pharmacology and Regenerative Medicine, The University of Illinois at Chicago, Chicago, IL Departments of ∥Pathology and Immunology #Radiation Oncology, Washington University School of Medicine, St. Louis, MO.
Early studies estimate that 5% to 10% of oropharyngeal squamous cell carcinomas overexpress p16 but are unassociated with transcriptionally-active high-risk human papillomavirus (HPV). Patients with discordant HPV testing may experience clinical outcomes that differ from traditional expectations. To document the rate of p16 and HPV mRNA positivity, characterize patients with discordant testing, and identify features that may warrant selective use of HPV-specific testing after p16 IHC, a multi-institutional, retrospective review of oropharyngeal squamous cell carcinoma patients with p16 IHC and HPV mRNA testing by reverse transcriptase polymerase chain reaction was performed. Read More