51 results match your criteria rhsod


Persistent Pulmonary Hypertension of the Newborn: Pathophysiological Mechanisms and Novel Therapeutic Approaches.

Front Pediatr 2020 24;8:342. Epub 2020 Jul 24.

Department of Surgery and Physiology, Cardiovascular Research and Development Center-UnIC, Faculty of Medicine, University of Porto, Porto, Portugal.

Persistent pulmonary hypertension of the newborn (PPHN) is one of the main causes of neonatal morbidity and mortality. It is characterized by sustained elevation of pulmonary vascular resistance (PVR), preventing an increase in pulmonary blood flow after birth. The affected neonates fail to establish blood oxygenation, precipitating severe respiratory distress, hypoxemia, and eventually death. Read More

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Recombinant Human Superoxide Dismutase and -Acetylcysteine Addition to Exogenous Surfactant in the Treatment of Meconium Aspiration Syndrome.

Molecules 2019 Mar 5;24(5). Epub 2019 Mar 5.

Department of Physiology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4C, 03601 Martin, Slovakia.

This study aimed to evaluate the molecular background of -acetylcysteine (NAC) and recombinant human superoxide dismutase (rhSOD) antioxidant action when combined with exogenous surfactant in the treatment of meconium aspiration syndrome (MAS), considering redox signalling a principal part of cell response to meconium. Young New Zealand rabbits were instilled with meconium suspension (Mec) and treated by surfactant alone (Surf) or surfactant in combination with i.v. Read More

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Modified porcine surfactant enriched by recombinant human superoxide dismutase for experimental meconium aspiration syndrome.

Life Sci 2018 Jun 21;203:121-128. Epub 2018 Apr 21.

Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), Biomedical Center Martin JFM CU, Slovakia.

Aims: Combination of exogenous surfactant with antioxidant enzyme recombinant human superoxide dismutase (rhSOD) was tested in the treatment of experimental meconium aspiration syndrome as oxidative processes play key role in its pathogenesis.

Material And Methods: Young New Zealand rabbits were instilled by saline (Sal group) or by meconium suspension (Mec group). Some of meconium-instilled animals were treated by surfactant alone (Surf group) or surfactant in combination with rhSOD (Surf + SOD group) and oxygen-ventilated for 5 h. Read More

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Cyclic stretch induces inducible nitric oxide synthase and soluble guanylate cyclase in pulmonary artery smooth muscle cells.

Int J Mol Sci 2013 Feb 21;14(2):4334-48. Epub 2013 Feb 21.

Department of Pediatrics, Northwestern University, Chicago, IL 60611, USA.

In the pulmonary vasculature, mechanical forces such as cyclic stretch induce changes in vascular signaling, tone and remodeling. Nitric oxide is a potent regulator of soluble guanylate cyclase (sGC), which drives cGMP production, causing vasorelaxation. Pulmonary artery smooth muscle cells (PASMCs) express inducible nitric oxide synthase (iNOS), and while iNOS expression increases during late gestation, little is known about how cyclic stretch impacts this pathway. Read More

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February 2013

Reduction of retinopathy of prematurity in extremely low gestational age newborns treated with recombinant human Cu/Zn superoxide dismutase.

Neonatology 2012 15;102(2):139-44. Epub 2012 Jun 15.

Department of Newborn Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.

Background: Reactive oxygen species have been implicated in the pathogenesis of retinopathy of prematurity (ROP). Extremely low gestational age (GA) newborns (ELGANs) have the highest risk of ROP and might benefit most from treatment with antioxidants.

Objectives: To determine whether recombinant human Cu/Zn superoxide dismutase (rhSOD) decreases the incidence or severity of ROP in ELGANs. Read More

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December 2012

Human pharmacokinetics of intravenous recombinant human Cu/Zn superoxide dismutase.

Int J Clin Pharmacol Ther 2012 Jun;50(6):413-7

Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria.

Objective: Oxidative stress plays an important role in human disease, but antioxidant therapies are limited. Under physiological conditions superoxide is controlled by the enzyme superoxide dismutase. A recombinant human Cu/Zn superoxide dismutase (rhSOD) might open new therapeutic possibilities. Read More

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SOD and inhaled nitric oxide normalize phosphodiesterase 5 expression and activity in neonatal lambs with persistent pulmonary hypertension.

Am J Physiol Lung Cell Mol Physiol 2010 Jul 16;299(1):L109-16. Epub 2010 Apr 16.

Dept. of Pediatrics, Northwestern Univ. Feinberg School of Medicine, 310 E. Superior St., Morton 4-685D, Chicago, IL 60611, USA.

Phosphodiesterase 5 (PDE5) and soluble guanylate cyclase (sGC) are key regulators of cGMP and pulmonary vascular tone. We sought to determine the impact of mechanical ventilation with O(2) with or without inhaled nitric oxide (iNO) or recombinant human Cu/Zn SOD (rhSOD) on sGC, PDE5, and cGMP in the ovine ductal ligation model of persistent pulmonary hypertension of the newborn (PPHN). PPHN lambs were ventilated with 100% O(2) for 24 h alone or combined with either inhalation of 20 parts per million (ppm) iNO continuously or a single intratracheal dose of rhSOD (5 mg/kg). Read More

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Economic evaluation of recombinant human copper zinc superoxide dismutase administered at birth to premature infants.

J Perinatol 2009 May 19;29(5):364-71. Epub 2009 Feb 19.

Division of Newborn Medicine, Harvard Medical School, Boston, MA, USA.

Objective: To determine the cost-effectiveness of recombinant human superoxide dismutase (rhSOD) in the prevention of chronic respiratory morbidity, defined as use of respiratory medications, in preterm infants.

Study Design: This retrospective economic evaluation was undertaken using data from a previously published randomized controlled trial of the use of rhSOD in neonates of birthweight 600 to 1200 g. This ancillary study measured all relevant direct medical costs from birth to 1 year corrected age using resource data collected for infants from the clinical trial. Read More

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Superoxide dismutase restores eNOS expression and function in resistance pulmonary arteries from neonatal lambs with persistent pulmonary hypertension.

Am J Physiol Lung Cell Mol Physiol 2008 Dec 12;295(6):L979-87. Epub 2008 Sep 12.

Dept. of Pediatrics, Northwestern Univ. Feinberg School of Medicine, 303 E. Chicago Ave., Ward 12-196, Chicago, IL 60611, USA.

Endothelial nitric oxide (NO) synthase (eNOS) expression and activity are decreased in fetal lambs with persistent pulmonary hypertension (PPHN). We sought to determine the impact of mechanical ventilation with O(2) with or without inhaled NO (iNO) or recombinant human SOD (rhSOD) on eNOS in the ductal ligation model of PPHN. PPHN lambs and age-matched controls were ventilated with 100% O(2) for 24 h alone or combined with 20 ppm iNO continuously or a single dose of rhSOD (5 mg/kg) given intratracheally at delivery. Read More

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December 2008

Increased biological half-life of aerosolized liposomal recombinant human Cu/Zn superoxide dismutase in pigs.

J Aerosol Med Pulm Drug Deliv 2008 Sep;21(3):281-90

Orthopaedic Department, University Hospital Basel, Basel, Switzerland.

Reactive oxygen species (ROS) are dangerous intermediates of cellular oxygen metabolisms, and are involved in pathogenesis of a wide range of diseases. Superoxide Dismutases (SODs) are an important part of antioxidant defense systems in mammalian cells capable of reducing the harmful effect of ROS on human tissues. Unfortunately, intravenously administered SOD shows a biological half-life of a few minutes, and enteral administration fails due to biodegradation of the enzyme in the gastrointestinal system. Read More

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September 2008

Cloning and characterization of a thermostable superoxide dismutase from the thermophilic bacterium Rhodothermus sp. XMH10.

J Ind Microbiol Biotechnol 2008 Feb 7;35(2):133-9. Epub 2007 Nov 7.

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen, 361005, People's Republic of China.

A superoxide dismutase (SOD) gene was cloned from the thermophilic bacterium Rhodothermus sp. XMH10 for the first time and highly expressed in Escherichia coli. The Rhodothermus sp. Read More

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February 2008

Superoxide dismutase improves oxygenation and reduces oxidation in neonatal pulmonary hypertension.

Am J Respir Crit Care Med 2006 Dec 28;174(12):1370-7. Epub 2006 Sep 28.

Department of Pediatrics, State University of New York at Buffalo, NY, USA.

Rationale: Hyperoxic ventilation in the management of persistent pulmonary hypertension of the newborn (PPHN) can result in the formation of reactive oxygen species, such as superoxide anions, which can inactivate nitric oxide (NO) and cause vasoconstriction and oxidation.

Objective: To compare the effect of intratracheal recombinant human superoxide dismutase (rhSOD) and/or inhaled NO (iNO) on systemic oxygenation, contractility of pulmonary arteries (PAs), and lung reactive oxygen species (isoprostane, 3-nitrotyrosine) levels in neonatal lambs with PPHN.

Methods: Six newborn lambs with PPHN (induced by antenatal ductal ligation) were killed at birth. Read More

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December 2006

Perfluorochemical liquids enhance delivery of superoxide dismutase to the lungs of juvenile rabbits.

Pediatr Res 2006 Jul 11;60(1):65-70. Epub 2006 May 11.

Division of Neonatology , Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.

Previous studies suggest acute lung injury (ALI) in premature newborns is associated with relative deficiency of antioxidant enzymes that may be ameliorated by recombinant human superoxide dismutase (rhSOD). Perfluorochemicals (PFCs) are distributed homogeneously and support gas exchange in diseased lungs. We investigated whether PFCs could provide an effective delivery system for rhSOD. Read More

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[Purification and renaturation of recombinant human Cu, Zn-SOD by metal-chelating affinity chromatography].

Sheng Wu Gong Cheng Xue Bao 2005 Nov;21(6):993-7

College of Life Sciences, Hebei University, Baoding 071002, China.

Overexpression of recombinant Human Cu, Zn-Superoxide Dismutase (rhCu, Zn-SOD) in E. coli results in the form of insoluble inclusion body. Purity of rhSOD inclusion body was over 80% by isolation and purification. Read More

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November 2005

[Protective effects and mechanisms of recombinant human superoxide dismutase in acute lung injury of rats following meconium aspiration].

Zhonghua Er Ke Za Zhi 2004 Oct;42(10):777-81

Neonatal Intensive Care Unit, Department of Pediatrics, Zhejiang University School of Medicine, Hangzhou 310003, China.

Objective: To evaluate the protective effects of recombinant human superoxide dismutase (rhSOD) in acute lung injury (ALI) following meconium aspiration.

Methods: Thirty-two healthy male Sprage-Dawley rats were divided into two groups, 8 were used as control (saline group) by infusing 1 ml/kg saline through endotracheal tube; the other 24 rats were used to establish model of ALI by infusing 1 ml/kg of 20% human newborn meconium suspension through endotracheal tube, and then were randomized to 3 groups (8 each): meconium group with no administration of saline or rhSOD; meconium + saline group by infusing 1 ml/kg saline through endotracheal tube; meconium + rhSOD group by infusing 20 mg/kg rhSOD dissolved in 1 ml/kg saline through endotracheal tube. The rats were killed 24 h after treatment. Read More

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October 2004

Intracellular monitoring of superoxide dismutase expression in an Escherichia coli fed-batch cultivation using on-line disruption with at-line surface plasmon resonance detection.

Anal Biochem 2005 Jul;342(1):152-9

Division of Biotechnology, IFM, Linköping University, 581 83 Linkoping, Sweden.

An on-line cell disruption system for at-line monitoring of the intracellular concentration of recombinant human superoxide dismutase (rhSOD) in a genetically modified Escherichia coli strain, HMS174(DE3) (pET11a/rhSOD), in bioreactor cultivations is described. The sampled bacteria were disrupted on-line by rapid mixing with a nonionic detergent. The recombinant protein content of the lysed bacterial sample was quantitated by a subsequent surface plasmon resonance biosensor with a specific monoclonal antibody. Read More

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Superoxide dismutase improves gas exchange and pulmonary hemodynamics in premature lambs.

Am J Respir Crit Care Med 2005 Sep 9;172(6):745-9. Epub 2005 Jun 9.

Department of Pediatrics, Pediatric Heart-Lung Center, Section of Neonatology, University of Colorado School of Medicine, Denver, Colorado, USA.

Rationale: Oxidant stress may increase the severity of respiratory distress syndrome (RDS) after premature birth by altering vasoreactivity and increasing lung edema, but the acute effects of superoxide dismutase (SOD) treatment on gas exchange, lung compliance (CL), and pulmonary vascular resistance in premature animals with RDS are unknown.

Objective: We studied the effects of intratracheal recombinant human SOD treatment (rhSOD) on gas exchange, CL, and pulmonary hemodynamics in 46 premature lambs with RDS.

Methods: After C-section delivery, lambs were randomly assigned to treatment with SOD (2. Read More

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September 2005

[Anti-inflammation and anti-oxidation effects of recombinant human superoxide dismutase on acute lung injury induced by meconium aspiration in infant rats].

Zhejiang Da Xue Xue Bao Yi Xue Ban 2005 Jan;34(1):55-9

The Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.

Objective: To investigate the anti-inflammation and anti-oxidation effects of recombinant human CuZn superoxide dismutase(rhSOD) on acute lung injury (ALI) induced by meconium aspiration in rats.

Methods: 1 ml/kg of 20% human newborn meconium suspension was intratracheally (IT) administrated to induce the model of ALI in 32 male Sprage-Dawley rats, and the animals were then randomized to 4 groups: 3 treatment groups with IT administration of 5, 10 and 20 mg/kg rhSOD dissolved in 1 ml/kg saline and the control group with IT administration of 1 ml/kg saline. The animals were killed after 24 h of treatments. Read More

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January 2005

Evaluation of disruption methods for the release of intracellular recombinant protein from Escherichia coli for analytical purposes.

Biotechnol Appl Biochem 2004 Aug;40(Pt 1):83-8

Division of Biotechnology, Department of Physics and Measurement Technology, Linköping University, SE-581 83 Linköping, Sweden.

The aim of the present study was to find disruption methods that allow fast and reproducible measurement of intracellular recombinant proteins with potential for on-line application. Production of rhSOD (recombinant human superoxide dismutase) by Escherichia coli was used as a model. Three methods of cell disruption, sonication, osmotic shock and chemical treatment using a non-ionic surfactant, were critically compared with respect to efficiency and reproducibility of the release of rhSOD. Read More

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Off-line monitoring of bacterial stress response during recombinant protein production using an optical biosensor.

J Biotechnol 2004 Jul;111(2):191-201

Division of Biotechnology, Department of Physics and Measurement Technology, Linköping University, 581 83 Linköping, Sweden.

A surface plasmon resonance (SPR) biosensor was used to monitor the profiles of the heat-shock protein (DnaK) and the expression of a heterologous protein to map the dynamics of the cellular stress response in Escherichia coli. As expression system was used an E. coli strain overproducing human recombinant superoxide dismutase (rhSOD). Read More

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Effects of recombinant human superoxide dismutase during reoxygenation with 21% or 100% oxygen after cerebral asphyxia in newborn piglets.

J Matern Fetal Neonatal Med 2003 Aug;14(2):96-101

Department of Pediatric Research, The National Hospital, Oslo, Norway.

Objective: Superoxide radicals (O2-) are generated during reoxygenation following asphyxia, possibly more when higher concentrations of O2 are used during resuscitation. Superoxide dismutase (SOD) is an antioxidant enzyme, which scavenges O2-. We tested the hypothesis that a single intravenous dose of recombinant human Cu,Zn SOD (rhSOD) could influence microcirculation and biochemical markers of asphyxia in piglets reoxygenated with 21% or 100% O2 after combined cerebral hypoxemia-ischemia-hypercapnia. Read More

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Metabolic analysis of the synthesis of high levels of intracellular human SOD in Saccharomyces cerevisiae rhSOD 2060 411 SGA122.

Biotechnol Bioeng 2003 Apr;82(2):152-69

Department of Chemical Engineering, Millennium Institute for Advanced Studies In Cell Biology and Biotechnology, University of Chile, Beauchef 861, Santiago, Chile.

The synthesis of human superoxide dismutase (SOD) in batch cultures of a Saccharomyces cerevisiae strain using a glucose-limited minimal medium was studied through metabolic flux analysis. A stoichiometric model was built, which included 78 reactions, according to metabolic pathways operative in these strains during respirofermentative and oxidative metabolism. It allowed calculation of the distribution of metabolic fluxes during diauxic growth on glucose and ethanol. Read More

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Effects of exogenous surfactant and recombinant human copper-zinc superoxide dismutase on oxygen-dependent antimicrobial defenses.

Biol Neonate 2002 Aug;82(2):96-102

Laboratoires de physiologie respiratoire, CHU Cochin Port-Royal, Paris, France.

The use of human recombinant CuZn superoxide dismutase (rhSOD) in addition to exogenous surfactant has been studied as a therapeutic strategy to prevent acute and chronic lung injury in premature infants with blood monocytes (MO). However, scavenging of superoxide by rhSOD may compromise bacterial killing by phagocytes. In the present study, we investigated the interaction of exogenous surfactant and rhSOD with the antibacterial activity of human blood MO. Read More

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Developmental differences in the role of interleukins in hyperoxic lung injury in animal models.

Authors:
Vineet Bhandari

Front Biosci 2002 Jul 1;7:d1624-33. Epub 2002 Jul 1.

Department of Pediatrics, Children's Hospital, Yale University School of Medicine, New Haven, CT 06520-8064, USA.

Interleukins (IL) are part of the group of immune mediators known as cytokines. IL are produced by many different cells and possess a wide spectrum of biological activities. This review will be focused on the role of IL-1 to 6, 8, 10-13 as it pertains to the effects of hyperoxia on the adult and newborn lung in animal models. Read More

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Anti-inflammatory effect of recombinant human superoxide dismutase in rats and mice and its mechanism.

Acta Pharmacol Sin 2002 May;23(5):439-44

National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.

Aim: To investigate the anti-inflammatory effects and mechanism of recombinant human superoxide dismutase (rhSOD).

Methods: Inflammation models such as croton oil-induced ear swelling and carrageenan-induced hind paw edema in mice and rats were prepared. The nitric oxide synthase (NOS ) activity was measured by NADPH-diaphoras stain assay, N-acetyl-beta-D-glucosaminidase (beta-NAG) activity by spectrophotography, malondialdehyde (MDA) content by thiobarbituric acid (TBA) fluorescence technique, and interleukin-1beta (IL -1beta), tumor necrosis factor alpha (TNF alpha), and IL-8 content by radioimmunoassay (RIA). Read More

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Recombinant human superoxide dismutase enhances the effect of inhaled nitric oxide in persistent pulmonary hypertension.

Am J Respir Crit Care Med 2001 Sep;164(5):834-9

Department of Pediatrics, Northwestern University, Chicago, Illinois, USA.

We investigated the pulmonary vascular effects of superoxide dismutase (SOD) alone and in combination with inhaled nitric oxide (iNO) in newborn lambs with persistent pulmonary hypertension (PPHN) following prenatal ligation of the ductus arteriosus. In in vitro experiments, pretreatment with SOD significantly enhanced vascular relaxation in response to the NO donor S-nitrosyl-acetylpenicillamine (SNAP) in fifth-generation pulmonary arteries isolated from lambs with PPHN. In vivo treatment of fully instrumented newborn lambs with a single intratracheal dose of recombinant human CuZn SOD (rhSOD; 5 mg/kg) produced selective dilation of the pulmonary circulation. Read More

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September 2001

Endothelial cell protection against ischemia/reperfusion injury by lecithinized superoxide dismutase.

Kidney Int 2001 Aug;60(2):786-96

Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.

Background: Organs used for transplantation may experience long periods of cold ischemic preservation and consequently oxygen free radical-mediated damage following reperfusion. Lecithinized superoxide dismutase (lec-SOD) is a novel free radical scavenger that has been shown to bind with high affinity to cell membranes. The aim of this study was to determine whether lec-SOD bound to endothelial cells under organ preservation conditions to mediate direct antioxidant activity at the endothelial cell surface and thus offer protection against the harmful effects of ischemia/reperfusion injury. Read More

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[Gene cloning, expression and purification of its production of recombinant human superoxide dismutase].

Sheng Wu Gong Cheng Xue Bao 2000 Sep;16(5):557-60

National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050.

Human SOD cDNA was cloned and constructed an expression plasmid with high sufficient and stabilility expression in E. coli. The rhSOD cDNA was amplified by RT-PCR with the template of the total RNA extracted from human liver tissue. Read More

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September 2000

Lecithinized copper, zinc-superoxide dismutase ameliorates prolonged hypoxia-induced injury of cardiomyocytes.

Free Radic Biol Med 2000 Jul;29(1):34-41

Department of Cardiovascular Medicine, University of Tokyo, Graduate School of Medicine, Tokyo, Japan.

Recent studies have suggested that prolonged hypoxia results in increased production of reactive oxygen species in cardiomyocytes, which leads to apoptosis of these cells. We previously showed that lecithinized recombinant human copper, zinc-superoxide dismutase (rhSOD) showed increased bioavailability through greater membrane affinity and a longer half-life than unmodified SOD. The purpose of this study was to investigate whether lecithinized SOD plays a protective role against hypoxic injury in cardiomyocytes. Read More

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Long-term follow-up of premature infants treated with prophylactic, intratracheal recombinant human CuZn superoxide dismutase.

J Perinatol 2000 Jun;20(4):213-6

Department of Pediatrics, Winthrop University Hospital, Mineola, NY 11501, USA.

Objective: To examine the long-term effects of treatment with recombinant human CuZn superoxide dismutase (rhSOD) in infants enrolled previously in two placebo-controlled trials.

Study Design: Records for 46 (88%) infants were examined, with 19 infants having received either single or multiple intratracheal (i.t. Read More

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