59,131 results match your criteria revealed strain

Chikungunya Fever in Southern Thailand, 2018.

Am J Trop Med Hyg 2021 Aug 2. Epub 2021 Aug 2.

Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Infection by the mosquito-borne chikungunya virus (CHIKV) causes acute febrile illness and debilitating arthralgia. Outbreaks are sometimes not recognized because of its clinical resemblance to the more common dengue fever ubiquitous in tropical countries. An upsurge of dengue-like illness was reported in Satun province located in southern Thailand during the rainy season in 2018. Read More

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Dual Circulation of Duck Hepatitis A Virus Genotypes 1 and 3 in Egypt.

Avian Dis 2021 Mar;65(1):1-9

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center, Giza, 12618 Egypt,

Duck hepatitis A virus (DHAV) causes acute hepatitis and mortality, resulting in high economic losses in the duck farm industry. The current study describes the outbreak of DHAV in vaccinated duck farms in North Egypt during 2019 and molecular characterization of the 3' untranslated region (UTR) and viral protein VP1 genes. The 30 samples were collected from 7- to 28-day-old commercial Pekin ducks that showed a history of nervous signs and sudden deaths and were on farms in 6 governorates. Read More

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Schumannella soli sp. nov., a novel actinomycete isolated from mangrove soil by in situ cultivation.

Antonie Van Leeuwenhoek 2021 Aug 2. Epub 2021 Aug 2.

Beijing Key Laboratory of Antimicrobial Agents, Department of Microbial Chemistry, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China.

A novel actinobacterial strain, designated 10F1D-1, was isolated from soil sample collected from Futian mangrove nature reserve, China using of the in situ cultivation technique. Preliminary analysis based on the 16S rRNA gene sequence revealed that strain 10F1D-1 was the member of genus Schumannella with sharing highest sequence similarity (99.7%) to Schumannella luteola DSM 23141. Read More

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Transcript pattern analysis of Arf-family genes in the phytopathogen Fusarium oxysporum f. sp. lycopersici reveals the role of Arl3 in the virulence.

Antonie Van Leeuwenhoek 2021 Aug 2. Epub 2021 Aug 2.

Laboratorio de Diferenciación Celular, Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, 58038, Morelia, Michoacán, Mexico.

Fusarium oxysporum f. sp. lycopersici is an important plant pathogen that has been used to understand the virulence mechanisms that soil inhabiting fungi exhibit during the infection process. Read More

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The full-length genome sequence of a novel mitovirus from Botryosphaeria dothidea, the causal agent of pear ring rot disease.

Arch Virol 2021 Aug 2. Epub 2021 Aug 2.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.

Here, we describe a novel mycovirus, tentatively designated as "Botryosphaeria dothidea mitovirus 3" (BdMV3), isolated from Botryosphaeria dothidea strain FJ, which causes pear ring rot disease in Fujian Province, China. The complete genome nucleotide sequence of BdMV3 is 2538 nt in length and contains a single 2070-nt open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) of 689 amino acids (aa) using the fungal mitochondrial genetic code. BLASTp analysis revealed that the RdRp of BdMV3 shares 28. Read More

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Ensuring communication access for all during the COVID-19 pandemic and beyond: supporting patients, providers, and caregivers in hospitals.

Augment Altern Commun 2021 Aug 2:1-13. Epub 2021 Aug 2.

Speech-Language Pathology Department, Munroe-Meyer Institute, University of Nebraska Medical Center, Omaha, NE, USA.

The COVID-19 pandemic has revealed substantial gaps in communication access for patients across hospital settings. With the rise in positive cases globally, the need for communication enhancement strategies, including augmentative and alternative communication (AAC), has emerged as a critical need. Many resources exist that describe AAC assessment and intervention processes in the acute care setting; however, AAC service provision in the COVID-19 pandemic is fraught with many unanticipated challenges. Read More

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Lactobacillus plantarum Lp62 exerts probiotic effects against Gardnerella vaginalis ATCC 49154 in bacterial vaginosis.

Lett Appl Microbiol 2021 Aug 2. Epub 2021 Aug 2.

Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, 45662-900, Ilhéus, BA, Brazil.

The severe side-effects elicited by conventional antibiotic therapy and the recurrence of Bacterial vaginosis-associated bacteria and bacterial resistance have led to the development of novel alternative therapies, among which genital probiotics are widely used. In this study, we aimed to evaluate the antimicrobial activities of L. plantarum Lp62 and its supernatant against Gardnerella vaginalis, using both in vitro and in vivo approaches. Read More

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Nitric Oxide-Releasing Nanoparticles Are Similar to Efinaconazole in Their Capacity to Eradicate Biofilms.

Front Cell Infect Microbiol 2021 15;11:684150. Epub 2021 Jul 15.

Department of Medicine, Division of Infectious Diseases, Albert Einstein College of Medicine, Bronx, NY, United States.

Filamentous fungi such as and , the main causative agents of onychomycosis, have been recognized as biofilm-forming microorganisms. Nitric oxide-releasing nanoparticles (NO-np) are currently in development for the management of superficial and deep bacterial and fungal infections, with documented activity against biofilms. In this context, this work aimed to evaluate, for the first time, the anti- biofilm potential of NO-np using standard ATCC MYA-4438 and clinical BR1A strains and compare it to commonly used antifungal drugs including fluconazole, terbinafine and efinaconazole. Read More

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Riboregulation in the Major Gastric Pathogen .

Front Microbiol 2021 16;12:712804. Epub 2021 Jul 16.

Unité Pathogenèse de Helicobacter, CNRS UMR 2001, Département de Microbiologie, Institut Pasteur, Paris, France.

is a Gram-negative bacterial pathogen that colonizes the stomach of about half of the human population worldwide. Infection by is generally acquired during childhood and this bacterium rapidly establishes a persistent colonization. causes chronic gastritis that, in some cases, progresses into peptic ulcer disease or adenocarcinoma that is responsible for about 800,000 deaths in the world every year. Read More

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An Efficient Strategy for Obtaining Mutants by Targeted Gene Deletion in .

Front Microbiol 2021 14;12:699783. Epub 2021 Jul 14.

Centre d'Étude de la Forêt (CEF) and Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC, Canada.

The dimorphic fungus is the highly aggressive pathogen responsible for the current, highly destructive, pandemic of Dutch elm disease (DED). Genome and transcriptome analyses of this pathogen previously revealed that a large set of genes expressed during dimorphic transition were also potentially related to plant infection processes, which seem to be regulated by molecular mechanisms different from those described in other dimorphic pathogens. Then, can be used as a representative species to study the lifestyle of dimorphic pathogenic fungi that are not shared by the "model species" and In order to gain better knowledge of molecular aspects underlying infection process and symptom induction by dimorphic fungi that cause vascular wilt disease, we developed a high-throughput gene deletion protocol for . Read More

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Genome-Scale Screening and Combinatorial Optimization of Gene Overexpression Targets to Improve Cadmium Tolerance in .

Front Microbiol 2021 14;12:662512. Epub 2021 Jul 14.

CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Heavy metal contamination is an environmental issue on a global scale. Particularly, cadmium poses substantial threats to crop and human health. is one of the model organisms to study cadmium toxicity and was recently engineered as a cadmium hyperaccumulator. Read More

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All Work and No Play: Indigenous Women "Pulling the Weight" in Home Life.

Soc Serv Rev 2021 Jun;95(2):278-311

Fordham University.

The invisible labor of household management, including child care, housework, and financial responsibilities, is a contemporary form of historical oppression adding strain and contributing to mothers' role overload, depression, distress, and health impairments. The purpose of this article is to use the Framework of Historical Oppression, Resilience, and Transcendence to understand the experiences of gender dynamics in home life responsibilities among two Southeastern tribes. Reconstructive analysis from a critical ethnography with 436 participants revealed the following themes: (1) moms "mostly pulling the weight"; (2) women and child care: "We do it all," and men-"If they're there, they're there"; (3) financial imbalances; and (4) women's resilience and resistance. Read More

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Genome sequences of two strains of Lactococcus lactis subsp. cremoris with the same ancestry but a different capacity to produce exopolysaccharides.

J Gen Appl Microbiol 2021 Jul 30. Epub 2021 Jul 30.

Department of Science, Technology and Innovation, Kobe University.

Strains of Lactococcus lactis subsp. cremoris are used to produce yogurt containing exopolysaccharides with a sticky texture. When strain G3-2 producing exopolysaccharides was grown at elevated temperatures, a spontaneous mutant EPSC, which had lost exopolysaccharides biosynthesis, was isolated. Read More

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Development of an attenuated smallpox vaccine candidate: The KVAC103 strain.

Vaccine 2021 Jul 29. Epub 2021 Jul 29.

Division of Infectious Diseases, Sejong Institute of Health and Environment Research, Sejong City 30015, Republic of Korea. Electronic address:

Smallpox, a disease caused by the variola virus, is one of the most dangerous diseases and had killed numerous people before it was eradicated in 1980. However, smallpox has emerged as the most threatening bio-terrorism agent; as the first- and second-generation smallpox vaccines have been controversial and have caused severe adverse reactions, new demands for safe smallpox vaccines have been raised and some attenuated smallpox vaccines have been developed. We have developed a cell culture-based highly attenuated third-generation smallpox vaccine candidate KVAC103 strain by 103 serial passages of the Lancy-Vaxina strain derived from the Lister in Vero cells. Read More

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Decoding Thermal Depolarization Temperature in Bismuth Ferrite-Barium Titanate Relaxor Ferroelectrics with Large Strain Response.

ACS Appl Mater Interfaces 2021 Jul 31. Epub 2021 Jul 31.

Department of Materials Science, Sichuan University, Chengdu 610064, China.

Bismuth ferrite-barium titanate (BF-BT) ferroelectrics attract attention due to their multifunctional properties and potential applications in high-temperature piezodevices. The thermal depolarization temperature () of BF-BT-based relaxor ferroelectrics has a close correlation with electrical properties, which was only recently discovered and is not well recognized. This work is concerned with the thermal depolarization process in (0. Read More

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The ultrastructure of cartilage tissue and its swelling response in relation to matrix health.

J Anat 2021 Aug 1. Epub 2021 Aug 1.

Department of Chemical and Materials Engineering, University of Auckland, Auckland, New Zealand.

This multi-length scale anatomical study explores the influence of mild cartilage structural degeneration on the tissue swelling response. While the swelling response of cartilage has been studied extensively, this is the first study to reveal and correlate tissue microstructure and ultrastructure, with the swelling induced cartilage tissue strains. Cartilage sample strips (n = 30) were obtained from the distal-lateral quadrant of thirty mildly degenerate bovine patellae and, following excision from the bone, the cartilage strips were allowed to swell freely for 2 h in solutions of physiological saline and distilled water successively. Read More

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Whole-genome characterization and comparative genomics of a novel freshwater cyanobacteria species: Pseudanabaena punensis.

Mol Phylogenet Evol 2021 Jul 28:107272. Epub 2021 Jul 28.

Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX, USA. Electronic address:

Cyanobacteria are emerging as a potential source of novel, beneficial bioactive compounds. However, some cyanobacteria species can harm water quality and public health through the production of toxins. Therefore, surveying the occurrence and generating genomic resources of cyanobacteria producing harmful compounds could help develop the control methods necessary to manage their growth and limit the release contaminants into the water bodies. Read More

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Post-process optimization of 3D printed poly(lactic-co-glycolic acid) dental implant scaffold for enhanced structure and mechanical properties: effects of sonication duration and power.

J Mater Sci Mater Med 2021 Jul 31;32(8):91. Epub 2021 Jul 31.

Newcastle University in Singapore, [email protected], 172A Ang Mo Kio Avenue 8 #05-01, Singapore, 567739, Singapore.

We described a technique of a post-process stage to partially remove the poly(vinyl alcohol) (PVA) binder in Poly(lactic-co-glycolic acid) (PLGA) dental scaffolds. The scaffolds were exposed to ultrasonic waves while immersed in an ethanol/acetone solvent mixture that possessed both polar and nonpolar properties. A factorial experiment was conducted in which the scaffolds were treated to three levels of sonication power (p): 0, 20% (22 W) and 40% (44 W), and soaking duration (t): 5, 15, and 30 min. Read More

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Green synthesized silver nanoparticles (AgNPs) from Parrotiopsis jacquemontiana (Decne) Rehder leaf extract and its biological activities.

Microsc Res Tech 2021 Jul 30. Epub 2021 Jul 30.

Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Parrotiopsis jacquemontiana (Decne) Rehder aqueous extract of leaf was used for biosynthesis of AgNPs and characterized through UV-Visible spectroscopy, X-ray diffraction, Fourier-transform-infrared, diffraction light scattering, and scanning electron microscope analysis. Moderate to strong antioxidant activity during in vitro antioxidant assays for scavenging of 2,2-diphenyl-1-picryl hydrazyl (DPPH), hydroxyl (OH), nitric oxide (NO) radicals, iron chelation, and inhibition of β-carotene bleaching was recorded with minimum IC value (27.70 ± 2. Read More

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Echocardiographic Reference Ranges of Non-invasive Myocardial Work Indices in Children.

Pediatr Cardiol 2021 Jul 30. Epub 2021 Jul 30.

Tam Duc Heart Hospital, 4 Nguyen Luong Bang, Tan Phu, Quận 7, Ho Chi Minh City, Vietnam.

Global myocardial work (GMW) is an emerging method to characterize left ventricle (LV) function with potential advantages over both ejection fraction and global longitudinal strain (GLS). We aimed to determine the feasibility and reproducibility for echocardiographic-derived GMW in a healthy pediatric population; establish normal reference values; and investigate the influence of age, gender, and other clinical factor on normal reference ranges. We prospectively enrolled 212 individuals (median age of 9 years; interquartile range, 6 to 12 years, 112 female). Read More

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CMR-based T1-mapping offers superior diagnostic value compared to longitudinal strain-based assessment of relative apical sparing in cardiac amyloidosis.

Sci Rep 2021 Jul 30;11(1):15521. Epub 2021 Jul 30.

Department of Cardiology I, University Hospital Münster, Albert-Schweitzer-Campus 1, Building A1, 48149, Münster, Germany.

Cardiac amyloidosis (CA) is an infiltrative disease. In the present study, we compared the diagnostic accuracy of cardiovascular magnetic resonance (CMR)-based T1-mapping and subsequent extracellular volume fraction (ECV) measurement and longitudinal strain analysis in the same patients with (a) biopsy-proven cardiac amyloidosis (CA) and (b) hypertrophic cardiomyopathy (HCM). N = 30 patients with CA, N = 20 patients with HCM and N = 15 healthy control patients without relevant cardiac disease underwent dedicated CMR studies. Read More

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Transcriptomics differentiate two novel bioactive strains of Paenibacillus sp. isolated from the perennial ryegrass seed microbiome.

Sci Rep 2021 Jul 30;11(1):15545. Epub 2021 Jul 30.

Agriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, VIC, Australia.

Paenibacillus species are Gram-positive bacteria that have been isolated from a diverse array of plant species and soils, with some species exhibiting plant growth-promoting (PGP) activities. Here we report two strains (S02 and S25) of a novel Paenibacillus sp. that were isolated from perennial ryegrass (Lolium perenne) seeds. Read More

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Maximizing the simultaneous production of lipids and carotenoids by Rhodosporidium toruloides from wheat straw hydrolysate and perspectives for large-scale implementation.

Bioresour Technol 2021 Jul 17;340:125598. Epub 2021 Jul 17.

Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, Building 223, 2800 Kongens Lyngby, Denmark. Electronic address:

This study aimed to select fermentation conditions able to simultaneously maximize the production of lipids and carotenoids by oleaginous yeast cultivated in wheat straw hydrolysate. An evolved strain of Rhodosporidium toruloides with improved tolerance to toxic compounds present in hydrolysate medium was used. Experiments were performed in order to investigate the effect of the temperature and inoculum load on the production of lipids and carotenoids by R. Read More

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Facile synthesis, antimicrobial and antiviral evaluation of novel substituted phenyl 1,3-thiazolidin-4-one sulfonyl derivatives.

Bioorg Chem 2021 Jul 9;114:105153. Epub 2021 Jul 9.

Drug Design & Discovery Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh 211007, India. Electronic address:

A series of novel substituted phenyl 1, 3-thiazolidin-4-one sulfonyl derivatives 5 (a-t) were synthesized and screened for their in-vitro anti-microbial and anti-viral activity. The result of the anti-microbial assay demonstrated compounds 5d, 5f, 5g, 5h, 5i, 5j showed prominent inhibitory activity against all the tested Gram-positive and Gram-negative bacterial strains, while compounds 5g, 5j, 5o, 5p, 5q showed significant activity against the entire set of fungal strains as compared to standard drug Ampicillin and Clotrimazole, respectively. The antimicrobial study revealed that compounds having electron-withdrawing groups showed significant antimicrobial potency. Read More

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sp.nov., an anaerobic thermophilic iron(III)-reducing bacterium isolated from a deep-sea hydrothermal sulfide deposits.

Int J Syst Evol Microbiol 2021 Jul;71(7)

Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, No. 184, Daxue Road, Siming District, Xiamen 361005, Fujian, PR China.

A thermophilic, anaerobic, iron-reducing bacterium strain JL129W03 (=KCTC 15905=MCCC 1A14213) was isolated from a sulfide sample collected from the Daxi hydrothermal field (60.5° E, 6.4° N, 2919 m depth) on the Carlsberg Ridge, northwest Indian Ocean. Read More

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Occurrence of Crown Gall Disease on Japanese Spindle (Euonymus japonicas var. Green Rocket) Caused by Agrobacterium rosae in Iran.

Plant Dis 2021 Jul 30. Epub 2021 Jul 30.

University of Tehran, 48425, Department of Plant Protection, Tehran, Tehran, Iran (the Islamic Republic of);

Crown gall disease caused by diverse Agrobacterium species is one of the main biotic constraints in the ornamental plants industry in Iran (Mafakheri et al. 2017). In August 2019, Japanese spindle (Euonymus japonicus var. Read More

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in Slaughtered Sheep in High- and Low-Humidity Regions in the South of Iran: Molecular Prevalence and Genotype Identification.

Vet Med Int 2021 5;2021:5576771. Epub 2021 Jul 5.

Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.

is one of the most common meat-born zoonoses that infect all warm-blooded animals and humans. Sheep () is one of the main reservoirs of worldwide, and the infections induce various sequels, such as abortion and stillbirth. The present study aimed to identify the effects of humidity on the prevalence of in sheep in high- and low-humidity regions. Read More

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Core Genome MLST for Source Attribution of .

Front Microbiol 2021 13;12:703890. Epub 2021 Jul 13.

U.S. Food and Drug Administration, Center for Veterinary Medicine, Laurel, MD, United States.

species are among the leading foodborne bacterial agents of human diarrheal illness. The majority of campylobacteriosis has been attributed to (85% or more), followed by (5-10%). The distribution of and varies by host organism, indicating that the contribution to human infection may differ between isolation sources. Read More

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Comparison of Two Genomes From Different Clades Reveals Independent Evolution of Alpha-Amylase Duplication, Variation in Secondary Metabolism Genes, and Differences in Primary Metabolism.

Front Microbiol 2021 13;12:691296. Epub 2021 Jul 13.

Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, MA, United States.

Microbes (bacteria, yeasts, molds), in addition to plants and animals, were domesticated for their roles in food preservation, nutrition and flavor. is a domesticated filamentous fungal species traditionally used during fermentation of Asian foods and beverage, such as sake, soy sauce, and miso. To date, little is known about the extent of genome and phenotypic variation of isolates from different clades. Read More

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Translational GTPase BipA Is Involved in the Maturation of a Large Subunit of Bacterial Ribosome at Suboptimal Temperature.

Front Microbiol 2021 13;12:686049. Epub 2021 Jul 13.

School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.

BPI-inducible protein A (BipA), a highly conserved paralog of the well-known translational GTPases LepA and EF-G, has been implicated in bacterial motility, cold shock, stress response, biofilm formation, and virulence. BipA binds to the aminoacyl-(A) site of the bacterial ribosome and establishes contacts with the functionally important regions of both subunits, implying a specific role relevant to the ribosome, such as functioning in ribosome biogenesis and/or conditional protein translation. When cultured at suboptimal temperatures, the genomic deletion strain (Δ) exhibits defects in growth, swimming motility, and ribosome assembly, which can be complemented by a plasmid-borne supplementation or suppressed by the genomic deletion. Read More

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