29,638 results match your criteria revealed differentially


Peroxiredomin-4 ameliorates lipotoxicity-induced oxidative stress and apoptosis in diabetic cardiomyopathy.

Biomed Pharmacother 2021 Jun 11;141:111780. Epub 2021 Jun 11.

Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China. Electronic address:

Diabetic cardiomyopathy (DCM), one severe complication in the diabetes, leads to high mortality in the diabetic patients. However, the understanding of molecular mechanisms underlying DCM is far from completion. Herein, we investigated the disease-related differences in the proteomes of DCM based on db/db mice and verified the protective roles of peroxiredoxin-4 (Prdx4) in H9c2 cardiomyocytes treated by palmitic acid (PA). Read More

View Article and Full-Text PDF

Prediction and Boolean logical modelling of synergistic microRNA regulatory networks during reprogramming of male germline pluripotent stem cells.

Biosystems 2021 Jun 12;207:104453. Epub 2021 Jun 12.

Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Odisha 769008, India. Electronic address:

Unipotent male germline stem (GS) cells can undergo spontaneous reprogramming to germline pluripotent stem (GPS) cells during in vitro culture. In our previous study, we proposed a Boolean logical model of gene regulatory network (GRN) during reprogramming of GS cells to GPS cells. This study was designed to predict and model synergistic microRNA (miRNA) regulatory network during reprogramming of GS cells into GPS cells. Read More

View Article and Full-Text PDF

ALS-linked mutations impair UBQLN2 stress-induced biomolecular condensate assembly in cells.

J Neurochem 2021 Jun 15. Epub 2021 Jun 15.

Department of Biology, Syracuse University, Syracuse, NY, 13244, USA.

Mutations in Ubiquilin-2 (UBQLN2), a ubiquitin-binding shuttle protein involved in several protein quality control processes, can lead to amyotrophic lateral sclerosis (ALS). We previously found that wild-type UBQLN2 forms dynamic, membraneless biomolecular condensates upon cellular stress, and undergoes liquid-liquid phase separation in vitro. However, the impact of ALS-linked mutations on UBQLN2 condensate formation in cells is unknown. Read More

View Article and Full-Text PDF

Comparative transcriptome analysis provides insights into steviol glycoside synthesis in stevia (Stevia rebaudiana Bertoni) leaves under nitrogen deficiency.

Plant Cell Rep 2021 Jun 15. Epub 2021 Jun 15.

Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, No. 1 Qianhuhoucun Village, Zhongshan Gate, Nanjing, 210014, China.

Key Message: Transcriptome analysis revealed the potential mechanism of nitrogen regulating steviol glycosides synthesis via shifting of leaf carbon metabolic flux or inducing certain transcription factors. Nitrogen (N) plays key regulatory roles in both stevia (Stevia rebaudiana) growth and the synthesis of its functional metabolite steviol glycosides (SGs), but the mechanism by which this nutrient regulates SGs synthesis remains to be elucidated. To address this question, a pot experiment was performed in a greenhouse where stevia plants fertilized with N (the control as CK plants) and compared with plants without the supply of N. Read More

View Article and Full-Text PDF

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly-ADP-ribose polymerase (TIPARP/PARP7) catalytic mutant mice (TiparpH532A) exhibit increased sensitivity to TCDD-induced hepatotoxicity and lethality.

Toxicol Sci 2021 Jun 15. Epub 2021 Jun 15.

Department of Pharmacology and Toxicology, University of Toronto, Toronto, Canada.

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly-ADP-ribose polymerase (TIPARP/PARP7), an aryl hydrocarbon receptor (AHR) target gene and mono-ADP-ribosyltransferase, acts as part of a negative feedback loop to repress AHR signaling. This process is prevented by a single H532A mutation in TIPARP that destroys its catalytic activity. We hypothesized that the loss of TIPARP catalytic activity would increase sensitivity to TCDD-induced toxicity in vivo. Read More

View Article and Full-Text PDF

Bioinformatics analysis of the key potential ceRNA biomarkers in human thymic epithelial tumors.

Medicine (Baltimore) 2021 Jun;100(24):e26271

Department of Thoracic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang.

Background: Thymic epithelial tumors (TETs), originating from the thymic epithelial cells, are the most common primary neoplasms of the anterior mediastinum. Emerging evidence demonstrated that the competing endogenous RNAs (ceRNAs) exerted a crucial effect on tumor development. Hence, it is urgent to understand the regulatory mechanism of ceRNAs in TETs and its impact on tumor prognosis. Read More

View Article and Full-Text PDF

Liver Proteome Profile of Growth Restricted and Appropriately Grown Newborn Wistar Rats Associated With Maternal Undernutrition.

Front Endocrinol (Lausanne) 2021 28;12:684220. Epub 2021 May 28.

Second Department of Obstetrics and Gynaecology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.

Background: Fetal growth restriction (FGR) has been associated with adverse perinatal outcomes and epigenetic modifications that impact gene expression leading to permanent changes of fetal metabolic pathways and thereby influence development of disease in childhood and adult life. In this study, we investigated the result of maternal food restriction on liver protein expression in Wistar male newborn pups.

Materials & Methods: Ten (n = 10) timed pregnant Wistar rats on their 14th day of gestation were randomly assigned to either control (n = 4) or food restricted group (n = 6). Read More

View Article and Full-Text PDF

Targeting androgen receptor (AR) with antiandrogen Enzalutamide increases prostate cancer cell invasion yet decreases bladder cancer cell invasion via differentially altering the AR/circRNA-ARC1/miR-125b-2-3p or miR-4736/PPARγ/MMP-9 signals.

Cell Death Differ 2021 Jun 14. Epub 2021 Jun 14.

George Whipple Lab for Cancer Research, Departments of Pathology, Urology, Radiation Oncology and The Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, NY, USA.

Androgen-deprivation therapy (ADT) via targeting androgens/androgen receptor (AR) signals may suppress cell proliferation in both prostate cancer (PCa) and bladder cancer (BCa), yet its impact on the cell invasion of these two urological cancers remains unclear. Here we found targeting androgens/AR with either the recently developed antiandrogen Enzalutamide (Enz) or AR-shRNAs led to increase PCa cell invasion, yet decrease BCa cell invasion. Mechanistic dissection revealed that suppressing androgens/AR signals could result in differential alterations of the selective circular RNAs (circRNAs) as a result of differential endogenous AR transcription. Read More

View Article and Full-Text PDF

RNA and quantitative proteomic analysis of Dictyostelium knock-out cells lacking the core autophagy proteins ATG9 and/or ATG16.

BMC Genomics 2021 Jun 15;22(1):444. Epub 2021 Jun 15.

Center for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, 50931, Cologne, Germany.

Background: Autophagy is an evolutionary ancient mechanism that sequesters substrates for degradation within autolysosomes. The process is driven by many autophagy-related (ATG) proteins, including the core members ATG9 and ATG16. However, the functions of these two core ATG proteins still need further elucidation. Read More

View Article and Full-Text PDF

Global proteome response to Pb(II) toxicity in poplar using SWATH-MS-based quantitative proteomics investigation.

Ecotoxicol Environ Saf 2021 Jun 12;220:112410. Epub 2021 Jun 12.

Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing, China; International Cultivar Registration Center for Osmanthus, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. Electronic address:

Lead (Pb) toxicity is a growing serious environmental pollution that threatens human health and crop productivity. Poplar, as an important economic and ecological forest species, has the characteristics of fasting growth and accumulating heavy metals, which is a powerful model plant for phytoremediation. Here, a novel label-free quantitative proteomic platform of SWATH-MS was applied to detect proteome changes in poplar seedling roots following Pb treatment. Read More

View Article and Full-Text PDF

Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers.

PLoS One 2021 14;16(6):e0252804. Epub 2021 Jun 14.

Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America.

Streptococcus equi subsp. equi (SEE) is a host-restricted bacterium that causes the common infectious upper respiratory disease known as strangles in horses. Perpetuation of SEE infection appears attributable to inapparent carrier horses because it neither persists long-term in the environment nor infects other host mammals or vectors, and infection results in short-lived immunity. Read More

View Article and Full-Text PDF

Identification potential epigenetic biomarkers of a human immunodeficiency virus/tuberculosis co-infection based on weighted gene co-expression network analysis.

Microbiol Immunol 2021 Jun 14. Epub 2021 Jun 14.

Drug Clinical Trial Center, Gansu Wuwei Tumor Hospital, Wuwei, Gansu, People's Republic of China.

Tuberculosis (TB) is one of the most common opportunistic infections and a leading cause of death in patients infected with human immunodeficiency virus (HIV). However, conventional diagnostic tools have several limitations. The aim of this study was to screen key DNA methylated cytosine-phosphate-guanine dinucleotide (CpG) islands (CGIs) to identify potential diagnosis biomarkers in HIV mono-infected patients and HIV/TB co-infected patients based on a network analysis. Read More

View Article and Full-Text PDF

Cellular communication network factor 3 in cartilage development and maintenance.

J Cell Commun Signal 2021 Jun 14. Epub 2021 Jun 14.

Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.

Cellular communication network factor (CCN) 3 is one of the classical members of the CCN family, which are characterized by common molecular structures and multiple functionalities. Although this protein was discovered as a gene product overexpressed in a truncated form in nephroblastoma, recent studies have revealed its physiological roles in the development and homeostasis of mammalian species, in addition to its pathological association with a number of diseases. Cartilage is a tissue that creates most of the bony parts and cartilaginous tissues that constitute the human skeleton, in which CCN3 is also differentially produced to exert its molecular missions therein. Read More

View Article and Full-Text PDF

Pioneer plant species and fungal root endophytes in metal-polluted tailings deposited near human populations and agricultural areas in Northern Mexico.

Environ Sci Pollut Res Int 2021 Jun 14. Epub 2021 Jun 14.

Departamento El Hombre y su Ambiente, Universidad Autónoma Metropolitana-Xochimilco, Mexico City, Mexico.

As a consequence of industrial mining activity, high volumes of tailings are scattered around Mexico. Frequently, tailings contain heavy metals (HM) which entail threats against all organisms. The aim of this research was to identify plants and root fungal endophytes in polymetallic polluted tailings with the potential to be used in strategies of bioremediation. Read More

View Article and Full-Text PDF

Differential gene regulation in DAPT-treated Hydra reveals candidate direct notch signalling targets.

J Cell Sci 2021 Jun 14. Epub 2021 Jun 14.

Ludwig Maximilians-University Munich, Germany, Biocenter, 82152 Planegg-Martinsried, Großhaderner Str. 2, Germany.

In Hydra, Notch inhibition causes defects in head patterning and prevents differentiation of proliferating nematocyte progenitor cells into mature nematocytes. To understand the molecular mechanisms by which the Notch pathway regulates these processes we performed RNAseq and identified genes that are differentially regulated in response to 48 hours of treating the animals with the Notch-inhibitor DAPT. To identify candidate direct regulators of Notch-signalling, we profiled gene expression changes that occur during subsequent restoration of Notch-activity and performed promoter analyses to identify RBPJ transcription factor binding sites in the regulatory regions of Notch-responsive genes. Read More

View Article and Full-Text PDF

Construction of lncRNA-related ceRNA regulatory network in diabetic subdermal endothelial cells.

Authors:
Jiangbo Wan Bo Liu

Bioengineered 2021 Dec;12(1):2592-2602

Department of Burns and Surgery, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.

Long non-coding RNAs (lncRNAs) were considered to be involved in vascular complications in diabetes mellitus, but still only limited knowledge in this regard has been obtained. Herein, we further explored the roles of lncRNAs and mRNAs in diabetic vasculopathy (DV) through conducting bioinformatics analysis using data set downloaded from GEO database. The differentially expressed lncRNAs and mRNAs were identified by edge package. Read More

View Article and Full-Text PDF
December 2021

Novel artificial intelligent/neural network system for staging of nonalcoholic steatohepatitis.

Hepatol Res 2021 Jun 14. Epub 2021 Jun 14.

Department of Human Pathology, Graduate School of Medicine, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan.

Aim: To develop a novel noninvasive test using an artificial intelligence (AI)/neural network (NN) system (named Fibro-Scope) to determine the fibrosis stage in nonalcoholic steatohepatitis (NASH).

Methods: 324 and 110 patients with histologically diagnosed NAFLD were enrolled for training and validation studies, respectively. Two independent pathologists histologically diagnosed patients with NAFLD for the validation study. Read More

View Article and Full-Text PDF

A Prognostic DNA Damage Repair Genes Signature and Its Impact on Immune Cell Infiltration in Glioma.

Front Oncol 2021 28;11:682932. Epub 2021 May 28.

Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Objective: Glioma is the most frequent type of malignant cerebral tumors. DNA damage repair genes (DDRGs) play a crucial role in the development of cancer. In this study, we constructed a DDRGs signature and investigated the potential mechanisms involved in this disease. Read More

View Article and Full-Text PDF

The GRAS gene family in watermelons: identification, characterization and expression analysis of different tissues and root-knot nematode infestations.

PeerJ 2021 26;9:e11526. Epub 2021 May 26.

College of Life Sciences, Jiangxi Science & Technology Normal University, Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-Vitro Diagnostic Reagents and Devices of Jiangxi Province, Nanchang, Jiangxi, China.

The family of GRAS plant-specific transcription factor plays diverse roles in numerous biological processes. Despite the identification and characterization of GRAS genes family in dozens of plant species, until now, GRAS members in watermelon () have not been investigated comprehensively. In this study, using bioinformatic analysis, we identified 37 GRAS genes in the watermelon genome (ClGRAS). Read More

View Article and Full-Text PDF

Identification of potential gene signatures associated with osteosarcoma by integrated bioinformatics analysis.

PeerJ 2021 27;9:e11496. Epub 2021 May 27.

Department of Spine Surgery, Tianjin Union Medical Center, Tianjin, China.

Background: Osteosarcoma (OS) is the most primary malignant bone cancer in children and adolescents with a high mortality rate. This work aims to screen novel potential gene signatures associated with OS by integrated microarray analysis of the Gene Expression Omnibus (GEO) database.

Material And Methods: The OS microarray datasets were searched and downloaded from GEO database to identify differentially expressed genes (DEGs) between OS and normal samples. Read More

View Article and Full-Text PDF

Examining histone modification crosstalk using immobilized libraries established from ligation-ready nucleosomes.

Chem Sci 2020 Aug 20;11(34):9218-9225. Epub 2020 Aug 20.

Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology Thuwal 23955 Saudi Arabia

Chromatin signaling relies on a plethora of posttranslational modifications (PTM) of the histone proteins which package the long DNA molecules of our cells in reoccurring units of nucleosomes. Determining the biological function and molecular working mechanisms of different patterns of histone PTMs requires access to various chromatin substrates of defined modification status. Traditionally, these are achieved by individual reconstitution of single nucleosomes or arrays of nucleosomes in conjunction with modified histones produced by means of chemical biology. Read More

View Article and Full-Text PDF

Identification and Tissue-Specific Characterization of Novel SHOX-Regulated Genes in Zebrafish Highlights SOX Family Members Among Other Genes.

Front Genet 2021 27;12:688808. Epub 2021 May 27.

Department of Human Molecular Genetics, Institute of Human Genetics, University Hospital Heidelberg, Heidelberg, Germany.

deficiency causes a spectrum of clinical phenotypes related to skeletal dysplasia and short stature, including Léri-Weill dyschondrosteosis, Langer mesomelic dysplasia, Turner syndrome, and idiopathic short stature. SHOX controls chondrocyte proliferation and differentiation, bone maturation, and cellular growth arrest and apoptosis transcriptional regulation of its direct target genes , , and . However, our understanding of SHOX-related pathways is still incomplete. Read More

View Article and Full-Text PDF

Comprehensive Profiling Reveals Distinct Microenvironment and Metabolism Characterization of Lung Adenocarcinoma.

Front Genet 2021 28;12:619821. Epub 2021 May 28.

Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Lung adenocarcinoma has entered into an era of immunotherapy with the development of immune checkpoint inhibitors (ICIs). The identification of immune subtype is crucial to prolonging survival in patients. The tumor microenvironment (TME) and metabolism have a profound impact on prognosis and therapy. Read More

View Article and Full-Text PDF

Development and Validation of a Novel Metabolic-Related Signature Predicting Overall Survival for Pancreatic Cancer.

Front Genet 2021 28;12:561254. Epub 2021 May 28.

Liver Disease Center, The Affiliated Hospital of Qingdao University, Qingdao, China.

Recently, growing evidence has revealed the significant effect of reprogrammed metabolism on pancreatic cancer in relation to carcinogenesis, progression, and treatment. However, the prognostic value of metabolism-related genes in pancreatic cancer has not been fully revealed. We identified 379 differentially expressed metabolic-related genes (DEMRGs) by comparing 178 pancreatic cancer tissues with 171 normal pancreatic tissues in The Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression project (GTEx) databases. Read More

View Article and Full-Text PDF

Transcriptomic Analysis of Ovine Hepatic Lymph Node Following Infection - Inhibition of NK Cell and IgE-Mediated Signaling.

Front Immunol 2021 28;12:687579. Epub 2021 May 28.

UCD School of Veterinary Medicine, Dublin, Ireland.

is a trematode parasite responsible for major economic losses in livestock production, and is also a food-borne zoonotic agent in developing rural regions. For years, the immunoregulatory mechanisms employed by the parasite have hampered efforts to develop a successful vaccine candidate. Given that a comprehensive understanding of the immune response to infection is needed, we investigated the gene expression changes in ovine hepatic lymph nodes after experimental infection with . Read More

View Article and Full-Text PDF

Immunodominance of Epitopes and Protective Efficacy of HI Antigen Are Differentially Altered Using Different Adjuvants in a Mouse Model of Bacteremia.

Front Immunol 2021 27;12:684823. Epub 2021 May 27.

National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing, China.

HI, a fusion protein that consists of the alpha-toxin (Hla) and the N2 domain of iron surface determinant B (IsdB), is one of the antigens in the previously reported vaccine rFSAV and has already entered phase II clinical trials. Previous studies revealed that HI is highly immunogenic in both mice and healthy volunteers, and the humoral immune response plays key roles in HI-mediated protection. In this study, we further investigated the protective efficacy of immunization with HI plus four different adjuvants in a mouse bacteremia model. Read More

View Article and Full-Text PDF

Immune Infiltration Subtypes Characterization and Identification of Prognosis-Related lncRNAs in Adenocarcinoma of the Esophagogastric Junction.

Front Immunol 2021 28;12:651056. Epub 2021 May 28.

Department of Epidemiology and Biostatistics, National Clinical Research Center for Cancer, Key Laboratory of Molecular Cancer Epidemiology of Tianjin, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

The incidence of adenocarcinoma of the esophagogastric junction (AEG) has markedly increased worldwide. However, the precise etiology of AEG is still unclear, and the therapeutic options thus remain limited. Growing evidence has implicated long non-coding RNAs (lncRNAs) in cancer immunomodulation. Read More

View Article and Full-Text PDF

Attenuated Induction of the Unfolded Protein Response in Adult Human Primary Astrocytes in Response to Recurrent Low Glucose.

Front Endocrinol (Lausanne) 2021 26;12:671724. Epub 2021 May 26.

Institute of Biomedical and Clinical Sciences, University of Exeter Medical School, Exeter, United Kingdom.

Aims/hypothesis: Recurrent hypoglycaemia (RH) is a major side-effect of intensive insulin therapy for people with diabetes. Changes in hypoglycaemia sensing by the brain contribute to the development of impaired counterregulatory responses to and awareness of hypoglycaemia. Little is known about the intrinsic changes in human astrocytes in response to acute and recurrent low glucose (RLG) exposure. Read More

View Article and Full-Text PDF

Post-stroke Delivery of Valproic Acid Promotes Functional Recovery and Differentially Modifies Responses of Peri-Infarct Microglia.

Front Mol Neurosci 2021 28;14:639145. Epub 2021 May 28.

Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.

The specific role of peri-infarct microglia and the timing of its morphological changes following ischemic stroke are not well understood. Valproic acid (VPA) can protect against ischemic damage and promote recovery. In this study, we first determined whether a single dose of VPA after stroke could decrease infarction area or improve functional recovery. Read More

View Article and Full-Text PDF

Characterizing clinical features and location-specific gene expression profiles associated with pain burden in children with functional dyspepsia.

Neurogastroenterol Motil 2021 Jun 13:e14185. Epub 2021 Jun 13.

Department of Anesthesiology, University of Connecticut School of Nursing, University of Kansas School of Medicine, Storrs, CT, US.

Background: In children with functional dyspepsia (FD), genes involved in pain modulation may be differentially expressed contributing to chronic pain.

Methods: Children with suspected FD (cases) and known eosinophilic esophagitis (controls) undergoing esophagogastroduodenoscopy completed the Rome IV Diagnostic, Pain Burden and Frequency Severity-Duration questionnaires. Two antral and two duodenal biopsies were collected and relative fold differences in gene expression for 84 pain-associated genes compared to pain-free controls were calculated. Read More

View Article and Full-Text PDF