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Effects of Acute Dance and Aerobic Exercise on Drug Craving and Food Reward in Women with Methamphetamine Dependence.

Med Sci Sports Exerc 2021 Jun 10. Epub 2021 Jun 10.

School of Psychology, Shanghai University of Sport, Shanghai, People's Republic of China School of Psychology, Faculty of Medicine and Health, University of Leeds, Leeds, United Kingdom People's Liberation Army Second Military Medical University Naval Medical University, Department of Orthopedics, Changhai Hospital, Shanghai, People's Republic of China.

Introduction: Drug dependence causes an overestimation of drug-related stimuli and an underestimation of non-drug-related stimuli, such as food. The purpose of this study was to investigate the effects of acute moderate-intensity dance and aerobic exercise on drug craving, appetite, prefrontal neural activation to food cues and food reward in women with MA dependence.

Methods: Thirty-nine women who met the DSM-V MA dependence criteria participated in the experiment and were randomly assigned to either a dance (n = 20) or exercise (n = 19) group. Read More

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Photobiomodulation for non-exudative age-related macular degeneration.

Cochrane Database Syst Rev 2021 05 6;5:CD013029. Epub 2021 May 6.

Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK.

Background: Age-related macular degeneration (AMD) is one of the leading causes of blindness in high-income countries. The majority of cases of AMD are of the non-exudative type. Experts have proposed photobiomodulation (PBM) therapy as a non-invasive procedure to restore mitochondrial function, upregulate cytoprotective factors and prevent apoptotic cell death in retinal tissue affected by AMD. Read More

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Presbyopia - A Review of Current Treatment Options and Emerging Therapies.

Clin Ophthalmol 2021 24;15:2167-2178. Epub 2021 May 24.

Ophthalmic Consultants of Long Island, Garden City, NY, USA.

Presbyopia is a common age-related vision disorder characterized by a progressive inability to focus on near objects. If uncorrected or under-corrected, presbyopia can significantly impact patients' quality of life. Presbyopia represents an area of considerable unmet need due to its rising prevalence worldwide as the population ages, the high proportion of under-treated individuals in some parts of the world, and the limitations of currently available corrective methods. Read More

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Absence of significant off-target splicing variation with a U7snRNA vector targeting DMD exon 2 duplications.

Hum Gene Ther 2021 Jun 1. Epub 2021 Jun 1.

Nationwide Children's Hospital, Center for Gene Therapy, 700 Children's Drive, WA3014, Columbus, Ohio, United States, 43209;

Exon skipping therapies for Duchenne muscular dystrophy that restore an open reading frame can be induced by the use of non-coding U7 small nuclear RNA (U7snRNA) modified by an antisense exon-targeting sequence delivered by an adeno-associated virus (AAV) vector. We have developed an AAV vector (AAV9.U7-ACCA) containing four U7snRNAs targeting the splice donor and acceptor sites of dystrophin exon 2, resulting in highly efficient exclusion of DMD exon 2. Read More

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Pre-clinical dose-escalation studies establish a therapeutic range for U7snRNA-mediated exon 2 skipping.

Mol Ther Methods Clin Dev 2021 Jun 23;21:325-340. Epub 2021 Mar 23.

Center for Gene Therapy, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43205, USA.

Duchenne muscular dystrophy (DMD) is an X-linked progressive disease characterized by loss of dystrophin protein that typically results from truncating mutations in the DMD gene. Current exon-skipping therapies have sought to treat deletion mutations that abolish an open reading frame (ORF) by skipping an adjacent exon, in order to restore an ORF that allows translation of an internally deleted yet partially functional protein, as is seen with many patients with the milder Becker muscular dystrophy (BMD) phenotype. In contrast to that approach, skipping of one copy of a duplicated exon would be expected to result in a full-length transcript and production of a wild-type protein. Read More

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Development of a CRISPR-Cas9 Based Luciferase Turn-On System as Nonhomologous End Joining Pathway Reporter.

Chembiochem 2021 Jun 7;22(12):2177-2181. Epub 2021 May 7.

Department of Breast Cancer Pathology and Research Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin, 300060, P. R. China.

There is a need of a non-homologous end joining (NHEJ) pathway reporter system that facilitates screening and discovery of NHEJ chemical inhibitors. In this study, we developed a CRISPR-Cas9 based luciferase turn-on system as a NHEJ pathway reporter. By substituting nucleotide 205C with ATC, we introduced a reading-frame shift and a pre-stop codon into the luciferase coding region and thereby generated a bioluminescent signal mute HEK293T reporter cell line. Read More

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Olaparib in hormone receptor-positive, HER2-negative metastatic breast cancer with a somatic mutation.

Ther Adv Med Oncol 2021 5;13:17588359211006962. Epub 2021 Apr 5.

Department of Medical Affairs, Guardant Health, Inc., Redwood City, CA, USA.

The oral poly(adenosine diphosphate-ribose) polymerase inhibitor olaparib is approved for the treatment of patients with human epidermal growth factor 2-negative (HER2-) metastatic breast cancer (mBC) and a germline breast cancer susceptibility gene (BRCA) mutation who have been treated with chemotherapy. This case report describes a 63-year-old postmenopausal woman with somatic -mutated mBC who responded to olaparib treatment following multiple prior lines of therapy. The patient presented in January 2012 with locally advanced, hormone receptor-positive (HR+), HER2- BC which, despite initial response to neoadjuvant chemotherapy, recurred as bone disease in February 2014, and subsequently skin (June 2016) and liver (October 2016) metastases. Read More

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The impact of a mother-infant intervention on parenting and infant response to challenge: A pilot randomized controlled trial with adolescent mothers in El Salvador.

Infant Ment Health J 2021 May 11;42(3):400-412. Epub 2021 Apr 11.

Department of Psychology and Clinical Language Sciences, University of Reading, Reading, UK.

A pilot randomized controlled trial (RCT) was conducted in El Salvador of an intervention ('Thula Sana') previously shown to enhance maternal sensitivity and infant security of attachment in a South African sample. In El Salvador, trained community workers delivered the intervention from late pregnancy to 6 months postpartum as part of a home-visiting programme. The sample comprised 64 pregnant adolescent women, aged 14-19 years, living in predominantly rural settings. Read More

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Personalized Development of Antisense Oligonucleotides for Exon Skipping Restores Type XVII Collagen Expression in Junctional Epidermolysis Bullosa.

Int J Mol Sci 2021 Mar 24;22(7). Epub 2021 Mar 24.

Research Program for Molecular Therapy of Genodermatoses, EB House Austria, Department of Dermatology and Allergology, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria.

Intermediate junctional epidermolysis bullosa caused by mutations in the gene is characterized by the frequent development of blisters and erosions on the skin and mucous membranes. The rarity of the disease and the heterogeneity of the underlying mutations renders therapy developments challenging. However, the high number of short in-frame exons facilitates the use of antisense oligonucleotides (AON) to restore collagen 17 (C17) expression by inducing exon skipping. Read More

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Structural Perturbations of Exon-Skipping Edits within the Dystrophin D20:24 Region.

Biochemistry 2021 Mar 3;60(10):765-779. Epub 2021 Mar 3.

Department of Biology, Illinois Institute of Technology, 3101 South Dearborn Street, Chicago, Illinois 60616, United States.

Exon skipping is a disease-modifying therapy in which oligonucleotide analogues mask specific exons, eliminating them from the mature mRNA, and also the cognate protein. That is one possible therapeutic aim, but it can also be used to restore the reading frame for diseases caused by frameshift mutations, which is the case for Duchenne muscular dystrophy (DMD). DMD most commonly arises as a result of large exonic deletions that create a frameshift and abolish protein expression. Read More

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Correction of Frameshift Mutations in the atpB Gene by Translational Recoding in Chloroplasts of Oenothera and Tobacco.

Plant Cell 2021 Feb 9. Epub 2021 Feb 9.

Department Organelle Biology, Biotechnology and Molecular Ecophysiology, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany.

Translational recoding, also known as ribosomal frameshifting, is a process that causes ribosome slippage along the messenger RNA, thereby changing the amino acid sequence of the synthesized protein. Whether the chloroplast employs recoding is unknown. I-iota, a plastome mutant of Oenothera (evening primrose), carries a single adenine insertion in an oligoA stretch [11A] of the atpB coding region (encoding a β-subunit of the ATP synthase). Read More

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February 2021

Genome editing for Duchenne muscular dystrophy: a glimpse of the future?

Gene Ther 2021 Feb 2. Epub 2021 Feb 2.

Friedrich Baur Institute, Department of Neurology, LMU Munich, Munich, Germany.

Mutations in Dystrophin, one of the largest proteins in the mammalian body, are causative for a severe form of muscle disease, Duchenne Muscular Dystrophy (DMD), affecting not only skeletal muscle, but also the heart. In particular, exons 45-52 constitute a hotspot for DMD mutations. A variety of molecular therapies have been developed, comprising vectors encoding micro- and minidystrophins as well as utrophin, a protein with partially overlapping functions. Read More

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February 2021

Point mutation in the stop codon of increases the growth rate of subspecies .

Microbiology (Reading) 2021 02;167(2)

Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan.

subspecies (MAH) is a pathogen that causes various non-tuberculous mycobacterial diseases in humans and animals worldwide. Among the genus, MAH is characterized by relatively slow growth. Here, we isolated a rapidly growing variant of the MAH 104 strain. Read More

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February 2021

Bisamide Derivative of Dicarboxylic Acid Contributes to Restoration of Testicular Tissue Function and Influences Spermatogonial Stem Cells in Metabolic Disorders.

Front Cell Dev Biol 2020 3;8:562358. Epub 2020 Dec 3.

Laboratory of Regenerative Pharmacology, Goldberg ED Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre of the Russian Academy of Sciences, Tomsk, Russia.

Metabolic syndrome can lead to several challenging complications including degeneration of the pancreas and hypogonadism. Recently, we have shown that Bisamide Derivative of Dicarboxylic Acid (BDDA) can contribute to pancreatic restoration in mice with metabolic disorders via its positive effects on lipid and glucose metabolism, and by increasing the numbers of pancreatic stem cells. In the present study, we hypothesized that BDDA might also be effective in restoring hypogonadism caused by metabolic syndrome. Read More

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December 2020

Morpholino Oligomer-Induced Dystrophin Isoforms to Map the Functional Domains in the Dystrophin Protein.

Mol Ther Nucleic Acids 2020 Dec 21;22:263-272. Epub 2020 Aug 21.

Centre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth, WA 6150, Australia.

Dystrophin plays a crucial role in maintaining sarcolemma stability during muscle contractions, and mutations that prevent the expression of a functional protein cause Duchenne muscular dystrophy (DMD). Antisense oligonucleotide-mediated manipulation of pre-messenger RNA splicing to bypass Duchenne-causing mutations and restore functional dystrophin expression has entered the clinic for the most common mutations. The rationale of "exon skipping" is based upon genotype-phenotype correlations observed in Becker muscular dystrophy, a milder allelic disorder generally characterized by in-frame deletions and internally truncated but semi-functional dystrophin isoforms. Read More

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December 2020

Deletion of LORF9 but not LORF10 attenuates Marek's disease virus pathogenesis.

Vet Microbiol 2020 Dec 1;251:108911. Epub 2020 Nov 1.

Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, USA. Electronic address:

Marek's disease virus (MDV) genome contains a number of uncharacterized long open reading frames (LORF) and their role in viral pathogenesis has not been fully investigated. Among them, LORF9 (MDV069) and LORF10 (MDV071) are locate at the right terminus of the MDV genome unique long region (U). To investigate their role in MDV pathogenesis, we generated LORF9 or LORF10 deletion and revertant viruses. Read More

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December 2020

Towards a microbial process-based understanding of the resilience of peatland ecosystem service provisioning - A research agenda.

Sci Total Environ 2021 Mar 10;759:143467. Epub 2020 Nov 10.

Ecological Sciences, The James Hutton Institute, Craigiebuckler, Aberdeen AB15 8QH, UK.

Peatlands are wetland ecosystems with great significance as natural habitats and as major global carbon stores. They have been subject to widespread exploitation and degradation with resulting losses in characteristic biota and ecosystem functions such as climate regulation. More recently, large-scale programmes have been established to restore peatland ecosystems and the various services they provide to society. Read More

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[Vision restoration: science fiction or reality?]

Med Sci (Paris) 2020 Nov 5;36(11):1038-1044. Epub 2020 Nov 5.

Institut de la Vision, Sorbonne Université, Inserm et CNRS, 17 rue Moreau, 75012 Paris, France - Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, PA, États-Unis - Centre hospitalier national d'ophtalmologie (CHNO) des Quinze-Vingts, Département hospital-universitaire (DHU) Sight Restore, Inserm-DGOS CIC 1423, Paris, France - Fondation Ophtalmologique Rothschild, Paris, France.

Visual prostheses aim at restoring useful vision to patients who have become blind. This useful vision should enable them to regain autonomy in society for navigation, face recognition or reading. Two retinal prostheses have already obtained market authorization for patients affected by retinal dystrophies while a new device is in clinical trials for patients affected by age-related macular degeneration. Read More

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November 2020

Gene Editing of Muscle Stem Cells with Adeno-Associated Viral Vectors in a Mouse Model of Duchenne Muscular Dystrophy.

Mol Ther Methods Clin Dev 2020 Dec 28;19:320-329. Epub 2020 Sep 28.

Center for Advanced Genomic Technologies, Duke University, Durham, NC 27708, USA.

Delivery of therapeutic transgenes with adeno-associated viral (AAV) vectors for treatment of myopathies has yielded encouraging results in animal models and early clinical studies. Although certain AAV serotypes efficiently target muscle fibers, transduction of the muscle stem cells, also known as satellite cells, is less studied. Here, we used a Pax7nGFP;Ai9 dual reporter mouse to quantify AAV transduction events in satellite cells. Read More

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December 2020

Developments in reading frame restoring therapy approaches for Duchenne muscular dystrophy.

Expert Opin Biol Ther 2021 Mar 19;21(3):343-359. Epub 2020 Oct 19.

Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands.

Introduction: Exon skipping compounds restoring the dystrophin transcript reading frame have received regulatory approval for Duchenne muscular dystrophy (DMD). Recently, focus shifted to developing compounds to skip additional exons, improving delivery to skeletal muscle, and to genome editing, to restore the reading frame on DNA level.

Areas Covered: We outline developments for reading frame restoring approaches, challenges of mutation specificity, and optimizing delivery. Read More

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[Study of masking ability of laminate veneers for discolored teeth].

Beijing Da Xue Xue Bao Yi Xue Ban 2020 Oct;52(5):943-947

Objective: To select the most effective method among different masking treatments, such as different thickness and transparence, tissue surface's opaque coating, and opaque resin cement to restore discolored teeth esthetically by porcelain veneer.

Methods: Four extracted intact maxillary central incisors were prepared for porcelain veneer restoration and each three heat pressed porcelain veneers from three thicknesses (0.8 mm, 1. Read More

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October 2020

Molecular Cloning and Functional Analysis of the Homolog in Kiwifruit ().

Front Plant Sci 2020 16;11:551201. Epub 2020 Sep 16.

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China.

Kiwifruit bacterial canker, caused by the bacterial pathogen pv. (Psa), is a destructive disease in the kiwifruit industry globally. Consequently, understanding the mechanism of defense against pathogens in kiwifruit could facilitate the development of effective novel protection strategies. Read More

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September 2020

Golodirsen for Duchenne muscular dystrophy.

Authors:
S Anwar T Yokota

Drugs Today (Barc) 2020 Aug;56(8):491-504

Department of Medical Genetics, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada; The Friends of Garrett Cumming Research and Muscular Dystrophy Canada, HM Toupin Neurological Science Research Chair, Edmonton, Alberta, Canada.

Duchenne muscular dystrophy (DMD) is a life-shortening X-linked genetic disorder characterized by progressive wasting and weakening of muscles in boys. Loss-of-function mutations in the DMD gene, which codes for dystrophin, lead to this disease. The majority of mutations in this gene result in the exclusion of one or more exons from the transcript, eventually causing the remaining exons not to fit together correctly (i. Read More

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A Splice Intervention Therapy for Autosomal Recessive Juvenile Parkinson's Disease Arising from Parkin Mutations.

Int J Mol Sci 2020 Oct 1;21(19). Epub 2020 Oct 1.

Centre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth 6150, Australia.

Parkin-type autosomal recessive juvenile-onset Parkinson's disease is caused by mutations in the gene and accounts for 50% of all autosomal recessive Parkinsonism cases. Parkin is a neuroprotective protein that has dual functions as an E3 ligase in the ubiquitin-proteasome system and as a transcriptional repressor of . While genomic deletions of exon 3 disrupt the mRNA reading frame and result in the loss of functional parkin protein, deletions of both exon 3 and 4 maintain the reading frame and are associated with a later onset, milder disease progression, indicating this particular isoform retains some function. Read More

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October 2020

Sensitive and reliable evaluation of single-cut sgRNAs to restore dystrophin by a GFP-reporter assay.

PLoS One 2020 24;15(9):e0239468. Epub 2020 Sep 24.

Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Winston-Salem, North Carolina, United States of America.

Most Duchenne muscular dystrophy (DMD) cases are caused by deletions or duplications of one or more exons that disrupt the reading frame of DMD mRNA. Restoring the reading frame allows the production of partially functional dystrophin proteins, and result in less severe symptoms. Antisense oligonucleotide mediated exon skipping has been approved for DMD, but this strategy needs repeated treatment. Read More

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November 2020

Selective enhancement of low-gamma activity by tACS improves phonemic processing and reading accuracy in dyslexia.

PLoS Biol 2020 09 8;18(9):e3000833. Epub 2020 Sep 8.

Department of Neuroscience, University of Geneva, Geneva, Switzerland.

The phonological deficit in dyslexia is associated with altered low-gamma oscillatory function in left auditory cortex, but a causal relationship between oscillatory function and phonemic processing has never been established. After confirming a deficit at 30 Hz with electroencephalography (EEG), we applied 20 minutes of transcranial alternating current stimulation (tACS) to transiently restore this activity in adults with dyslexia. The intervention significantly improved phonological processing and reading accuracy as measured immediately after tACS. Read More

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September 2020

Executive functions and components of oral reading fluency through the lens of text complexity.

Read Writ 2020 Apr 11;33(4):1037-1073. Epub 2020 Feb 11.

Vanderbilt University, 1400 18th Avenue South, Nashville, TN 37203, USA.

As readers struggle to coordinate various reading- and language-related skills during oral reading fluency (ORF), miscues can emerge, especially when processing complex texts. Following a miscue, students often self-correct as a strategy to potentially restore ORF and online linguistic comprehension. Executive functions (EF) are hypothesized to play an interactive role during ORF. Read More

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Multimodality Approach to the Diagnosis and Assessment of Uveitic Macular Edema.

Ocul Immunol Inflamm 2020 Nov 20;28(8):1212-1222. Epub 2020 Aug 20.

Department of Ophthalmology, Fattouma Bourguiba University Hospital, Faculty of Medicine, University of Monastir , Monastir, Tunisia.

To provide an overview of the role of multimodality approach to the diagnosis and assessment of uveitic macular edema (UME). Review of literature. Optical coherence tomography (OCT) has become the gold standard in the detection, quantification, monitoring of treatment, and determination of prognosis in UME. Read More

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November 2020

Diminution in sperm quantity and quality in mouse models of Duchenne Muscular Dystrophy induced by a myostatin-based muscle growth-promoting intervention.

Eur J Transl Myol 2020 Jul 22;30(2):8904. Epub 2020 Jun 22.

School of Biological Sciences, University of Reading, UK.

Duchenne Muscular Dystrophy is a devastating disease caused by the absence of a functional rod-shaped cytoplasmic protein called dystrophin. Several avenues are being developed aimed to restore dystrophin expression in boys affected by this X-linked disease. However, its complete cure is likely to need combinational approaches which may include regimes aimed at restoring muscle mass. Read More

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Scarless Removal of Large Resistance Island AbaR Results in Antibiotic Susceptibility and Increased Natural Transformability in Acinetobacter baumannii.

Antimicrob Agents Chemother 2020 09 21;64(10). Epub 2020 Sep 21.

CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, CNRS UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, Villeurbanne, France.

With a great diversity in gene composition, including multiple putative antibiotic resistance genes, AbaR islands are potential contributors to multidrug resistance in However, the effective contribution of AbaR to antibiotic resistance and bacterial physiology remains elusive. To address this, we sought to accurately remove AbaR islands and restore the integrity of their insertion site. To this end, we devised a versatile scarless genome editing strategy. Read More

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September 2020