J Pediatr Gastroenterol Nutr 2021 Aug 30. Epub 2021 Aug 30.
Tropical Gastroenterology & Nutrition group, University of Zambia School of Medicine, Lusaka Blizard Institute, Barts & The London School of Medicine, Queen Mary University of London Section of Pediatric Nutrition, University of Colorado School of Medicine, Aurora, Colorado, USA.
Objectives: We measured fractional absorption of zinc (FAZ) in children with environmental enteropathy (EE) and carried out transcriptomic analysis of biopsies from these children in order to compare FAZ to histology of intestinal biopsies, expression of zinc transporter genes, and biomarkers of enteropathy.
Methods: Fractional absorption of a standardized aqueous dose of zinc was measured by a dual isotope ratio technique in a cohort of children aged between 9 and 24 months in Lusaka, Zambia, who all had non-responsive stunting. Gene expression analysis was carried out on biopsies through RNA sequencing using an Illumina HiSeq2000 platform. Read More