16,006 results match your criteria responses map

monitoring of tissue regeneration using a ratiometric lysosomal AIE probe.

Chem Sci 2020 Feb 11;11(12):3152-3163. Epub 2020 Feb 11.

HKUST-Shenzhen Research Institute No. 9 Yuexing 1st RD, South Area, Hi-tech Park, Nanshan Shenzhen 518057 China

Tissue regeneration is a crucial self-renewal capability involving many complex biological processes. Although transgenic techniques and fluorescence immunohistochemical staining have promoted our understanding of tissue regeneration, simultaneous quantification and visualization of tissue regeneration processes is not easy to achieve. Herein, we developed a simple and quantitative method for the real-time and non-invasive observation of the process of tissue regeneration. Read More

View Article and Full-Text PDF
February 2020

A Determinants-of-Fertility Ontology for Detecting Future Signals of Fertility Issues From Social Media Data: Development of an Ontology.

J Med Internet Res 2021 Jun 14;23(6):e25028. Epub 2021 Jun 14.

Department of Health Management, Samyook University, Seoul, Republic of Korea.

Background: South Korea has the lowest fertility rate in the world despite considerable governmental efforts to boost it. Increasing the fertility rate and achieving the desired outcomes of any implemented policies requires reliable data on the ongoing trends in fertility and preparations for the future based on these trends.

Objective: The aims of this study were to (1) develop a determinants-of-fertility ontology with terminology for collecting and analyzing social media data; (2) determine the description logics, content coverage, and structural and representational layers of the ontology; and (3) use the ontology to detect future signals of fertility issues. Read More

View Article and Full-Text PDF

Transcriptome-Wide N-Methyladenosine (mA) Profiling of Susceptible and Resistant Wheat Varieties Reveals the Involvement of Variety-Specific mA Modification Involved in Virus-Host Interaction Pathways.

Front Microbiol 2021 26;12:656302. Epub 2021 May 26.

State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo, China.

N-methyladenosine (mA) methylation is the most prevalent internal modification of post-transcriptional modifications in mRNA, tRNA, miRNA, and long non-coding RNA in eukaryotes. mA methylation has been proven to be involved in plant resistance to pathogens. However, there are no reports on wheat () mA transcriptome-wide map and its potential biological function in wheat resistance to wheat yellow mosaic virus (WYMV). Read More

View Article and Full-Text PDF

Regulation of Coronary Blood Flow by the Carotid Body Chemoreceptors in Ovine Heart Failure.

Front Physiol 2021 28;12:681135. Epub 2021 May 28.

Department of Physiology, University of Auckland, Auckland, New Zealand.

Carotid bodies (CBs) are peripheral chemoreceptors, which are primary sensors of systemic hypoxia and their activation produces respiratory, autonomic, and cardiovascular adjustments critical for body homeostasis. We have previously shown that carotid chemoreceptor stimulation increases directly recorded cardiac sympathetic nerve activity (cardiac SNA) which increases coronary blood flow (CoBF) in conscious normal sheep. Previous studies have shown that chemoreflex sensitivity is augmented in heart failure (HF). Read More

View Article and Full-Text PDF

Approaching an undergraduate medical curriculum map: challenges and expectations.

BMC Med Educ 2021 Jun 10;21(1):341. Epub 2021 Jun 10.

University College London, London, UK.

Background: Feedback received from medical students at University College London Medical School (UCLMS) suggested a lack of clarity regarding the contents and subsequent assessment of the undergraduate curriculum. In order to address these issues, a specialist team was established with the aim of designing and implementing a Curriculum Map (CM), which have been recognised in their ability to provide a centralised, visual representation of the curriculum. While multiple perspectives from educators to stakeholders can be considered here, the need for the CM to remain student centred was identified as key at UCLMS. Read More

View Article and Full-Text PDF

Stress effects on the reactive oxygen species (ROS)-dependent regulation of plant growth and development.

J Exp Bot 2021 Jun 9. Epub 2021 Jun 9.

School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, UK.

Plant growth is mediated by cell proliferation and expansion. Both processes are controlled by a network of endogenous factors such as phytohormones, reactive oxygen species (ROS), sugars and other signals, which influence gene expression and post translational regulation of proteins. Stress resilience requires rapid and appropriate responses in plant growth and development as well as defence. Read More

View Article and Full-Text PDF

Precise oculocentric mapping of transcranial magnetic stimulation-evoked phosphenes.

Neuroreport 2021 Jun 7. Epub 2021 Jun 7.

School of Optometry and Vision Science, University of Waterloo, Waterloo, Canada.

Objective: Transcranial magnetic stimulation (TMS)-evoked phosphenes are oculocentric; their perceived location depends upon eye position. We investigated the accuracy and precision of TMS-evoked phosphene oculocentric mapping.

Methods: We evoked central phosphenes by stimulating early visual cortical areas with TMS, systematically examining the effect of eye position by asking participants to report the location of the evoked phosphene. Read More

View Article and Full-Text PDF

Topographic numerosity maps cover subitizing and estimation ranges.

Nat Commun 2021 06 7;12(1):3374. Epub 2021 Jun 7.

Spinoza Centre for Neuroimaging, Amsterdam, Netherlands.

Numerosity, the set size of a group of items, helps guide behaviour and decisions. Non-symbolic numerosities are represented by the approximate number system. However, distinct behavioural performance suggests that small numerosities, i. Read More

View Article and Full-Text PDF

Multiple adjoining word- and face-selective regions in ventral temporal cortex exhibit distinct dynamics.

J Neurosci 2021 Jun 7. Epub 2021 Jun 7.

Center for Neuroscience at the University of Pittsburgh, University of Pittsburgh, A210 Langley Hall, Pittsburgh, PA 15260, USA.

The map of category-selectivity in human ventral temporal cortex (VTC) provides organizational constraints to models of object recognition. One important principle is lateral-medial response biases to stimuli that are typically viewed in the center or periphery of the visual field. However, little is known about the relative temporal dynamics and location of regions that respond preferentially to stimulus classes that are centrally viewed, like the face- and word-processing networks. Read More

View Article and Full-Text PDF

Attributes and generic competencies required of doctors: findings from a participatory concept mapping study.

BMC Health Serv Res 2021 Jun 7;21(1):560. Epub 2021 Jun 7.

Tasmanian School of Medicine, University of Tasmania, Locked bag 1377, Launceston, Tasmania, 7250, Australia.

Background: Medical education should ensure graduates are equipped for practice in modern health-care systems. Practicing effectively in complex health-care systems requires contemporary attributes and competencies, complementing core clinical competencies. These need to be made overt and opportunities to develop and practice them provided. Read More

View Article and Full-Text PDF

Heavy metal detoxification mechanisms by microalgae: Insights from transcriptomics analysis.

Environ Pollut 2021 May 27;285:117443. Epub 2021 May 27.

Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India; Centre for Transportation Systems, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India. Electronic address:

Heavy metal pollution in ecosystem is a global threat. The associated toxicity and carcinogenic nature of heavy metals/metalloids such as mercury, cadmium, lead, and arsenic are imposing a severe risk to both ecological diversity and human lives. Harnessing the adaptive feature of microalgae for remediating toxic heavy metal has reached a milestone in past few decades. Read More

View Article and Full-Text PDF

Robust estimation of SARS-CoV-2 epidemic in US counties.

Sci Rep 2021 06 4;11(1):11841. Epub 2021 Jun 4.

Department of Statistics and Applied Probability, University of California, Santa Barbara, CA, 93106, USA.

The COVID-19 outbreak is asynchronous in US counties. Mitigating the COVID-19 transmission requires not only the state and federal level order of protective measures such as social distancing and testing, but also public awareness of time-dependent risk and reactions at county and community levels. We propose a robust approach to estimate the heterogeneous progression of SARS-CoV-2 at all US counties having no less than 2 COVID-19 associated deaths, and we use the daily probability of contracting (PoC) SARS-CoV-2 for a susceptible individual to quantify the risk of SARS-CoV-2 transmission in a community. Read More

View Article and Full-Text PDF

Genetically targeted connectivity tracing excludes dopaminergic inputs to the interpeduncular nucleus from the ventral tegmentum and substantia nigra.

eNeuro 2021 Jun 2. Epub 2021 Jun 2.

Center for Integrative Brain Research, Seattle Children's Research Institute

The "habenulopeduncular system" consists of the medial habenula (MHb) and its principal target of innervation, the interpeduncular nucleus (IP). Neurons in the ventral MHb express acetylcholine along with glutamate, and both the MHb and IP are rich in nicotinic acetylcholine receptors. Much of the work on this system has focused on nicotinic mechanisms and their clinical implications for nicotine use, particularly because the IP expresses the α5 nicotinic receptor subunit, encoded by the CHRNA5 gene, which is genetically linked to smoking risk. Read More

View Article and Full-Text PDF

Comparison of endotracheal intubation, laryngeal mask airway, and I-gel in children undergoing strabismus surgery.

J Res Med Sci 2021 28;26. Epub 2021 Jan 28.

Poostchi Ophthalmology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: Insertion of the advanced airway during induction of general anesthesia can cause undesirable sympathetic stimulation such as increased intraocular pressure (IOP) and hemodynamic parameters. In this study, we compared insertion of three different advanced airway devices; endotracheal tube (ETT), laryngeal mask airway (LMA) and I-gel in terms of IOP, hemodynamic changes and postoperative nausea and vomiting (PONV) following induction of general anesthesia with propofol and remifentanil in children undergoing strabismus surgery.

Materials And Methods: A total of 90 children (5. Read More

View Article and Full-Text PDF
January 2021

The Chromatin Accessibility Landscape of Peripheral Blood Mononuclear Cells in Patients With Systemic Lupus Erythematosus at Single-Cell Resolution.

Front Immunol 2021 18;12:641886. Epub 2021 May 18.

Department of Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine, Shenzhen Engineering Research Center of Autoimmune Disease, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, China.

Objective: Systemic lupus erythematosus (SLE) is a complex autoimmune disease, and various immune cells are involved in the initiation, progression, and regulation of SLE. Our goal was to reveal the chromatin accessibility landscape of peripheral blood mononuclear cells (PBMCs) in SLE patients at single-cell resolution and identify the transcription factors (TFs) that may drive abnormal immune responses.

Methods: The assay for transposase accessible chromatin in single-cell sequencing (scATAC-seq) method was applied to map the landscape of active regulatory DNA in immune cells from SLE patients at single-cell resolution, followed by clustering, peak annotation and motif analysis of PBMCs in SLE. Read More

View Article and Full-Text PDF

A data-driven functional mapping of the anterior temporal lobes.

J Neurosci 2021 Jun 1. Epub 2021 Jun 1.

Section on Cognitive Neuropsychology, Laboratory of Brain and Cognition, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892.

Even though the anterior temporal lobe (ATL) comprises several anatomical and functional subdivisions, it is often reduced to a homogeneous theoretical entity, such as a domain-general convergence zone, or "hub", for semantic information. Methodological limitations are largely to blame for the imprecise mapping of function to structure in the ATL. There are two major obstacles to using fMRI to identify the precise functional organization of the ATL: the difficult choice of stimuli and tasks to activate, and dissociate, specific regions within the ATL and poor signal quality due to magnetic field distortions near the sinuses. Read More

View Article and Full-Text PDF

Shen-fu injection alleviates acute renal injury by reducing cytokine levels and modulating apoptosis in a porcine hemorrhagic shock model.

Acta Cir Bras 2021 28;36(4):e360405. Epub 2021 May 28.

PhD. Capital Medical University - Beijing ChaoYang Hospital - Department of Emergency - Beijing, China.

Purpose: Shen-fu injection (SFI) was used to intervene in the resuscitation of porcine hemorrhagic shock (HS) model to study its protective effects on acute kidney injury.

Methods: After 60 min of HS, 28 animals were randomly assigned into four groups. The groups were as follows: hemorrhagic shock group (HS); HS resuscitation with shed-blood group (HSR); HS resuscitation with shed-blood and SFI (1 mL·kg-1) group (HSR-SFI); and the sham operation group (Sham). Read More

View Article and Full-Text PDF

The Emerging Roles of JNK Signaling in Stem Cell Homeostasis.

Int J Mol Sci 2021 May 24;22(11). Epub 2021 May 24.

Department of Biochemistry and Molecular Pharmacology, New York University Grossman School of Medicine, New York, NY 10016, USA.

The Jun N-terminal kinase (JNK) pathway is an evolutionary conserved kinase cascade best known for its roles during stress-induced apoptosis and tumor progression. Recent findings, however, have identified new roles for this pleiotropic pathway in stem cells during regenerative responses and in cellular plasticity. Here, we provide an overview of recent findings about the new roles of JNK signaling in stem cell biology using two well-established models: the testis and the intestine. Read More

View Article and Full-Text PDF

Stress-Activated Protein Kinase Signalling Regulates Mycoparasitic Hyphal-Hyphal Interactions in .

J Fungi (Basel) 2021 May 6;7(5). Epub 2021 May 6.

Department of Microbiology, University of Innsbruck, 6020 Innsbruck, Austria.

is a mycoparasitic fungus used as biological control agent against fungal plant pathogens. The recognition and appropriate morphogenetic responses to prey-derived signals are essential for successful mycoparasitism. We established microcolony confrontation assays using strains expressing cell division cycle 42 (Cdc42) and Ras-related C3 botulinum toxin substrate 1 (Rac1) interactive binding (CRIB) reporters to analyse morphogenetic changes and the dynamic displacement of localized GTPase activity during polarized tip growth. Read More

View Article and Full-Text PDF

Detecting Attention Levels in ADHD Children with a Video Game and the Measurement of Brain Activity with a Single-Channel BCI Headset.

Sensors (Basel) 2021 May 6;21(9). Epub 2021 May 6.

Department of Experimental Psychology, Faculty of Psychology, Universidad de Sevilla, 41018 Seville, Spain.

Attentional biomarkers in attention deficit hyperactivity disorder are difficult to detect using only behavioural testing. We explored whether attention measured by a low-cost EEG system might be helpful to detect a possible disorder at its earliest stages. The GokEvolution application was designed to train attention and to provide a measure to identify attentional problems in children early on. Read More

View Article and Full-Text PDF

Integrated analysis of multimodal single-cell data.

Cell 2021 May 27. Epub 2021 May 27.

Center for Genomics and Systems Biology, New York University, New York, NY 10003, USA; New York Genome Center, New York, NY 10013, USA. Electronic address:

The simultaneous measurement of multiple modalities represents an exciting frontier for single-cell genomics and necessitates computational methods that can define cellular states based on multimodal data. Here, we introduce "weighted-nearest neighbor" analysis, an unsupervised framework to learn the relative utility of each data type in each cell, enabling an integrative analysis of multiple modalities. We apply our procedure to a CITE-seq dataset of 211,000 human peripheral blood mononuclear cells (PBMCs) with panels extending to 228 antibodies to construct a multimodal reference atlas of the circulating immune system. Read More

View Article and Full-Text PDF

The liver-clock coordinates rhythmicity of peripheral tissues in response to feeding.

Nat Metab 2021 May 31. Epub 2021 May 31.

Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.

The mammalian circadian system consists of a central clock in the brain that synchronizes clocks in the peripheral tissues. Although the hierarchy between central and peripheral clocks is established, little is known regarding the specificity and functional organization of peripheral clocks. Here, we employ altered feeding paradigms in conjunction with liver-clock mutant mice to map disparities and interactions between peripheral rhythms. Read More

View Article and Full-Text PDF

The role of the MAP kinase-kinase protein StMKK1 in potato immunity to different pathogens.

Hortic Res 2021 Jun 1;8(1):117. Epub 2021 Jun 1.

College of Horticulture, Northwest A&F University and Shaanxi Engineering Research Center for Vegetables, Yangling, Shaanxi, 712100, China.

Mitogen-activated protein kinase (MAPK) cascades play important roles in plant immunity. Previously, we reported that the potato StMKK1 protein negatively regulates Nicotiana benthamiana resistance to Phytophthora infestans. However, the functions of StMKK1 in potato immunity are unknown. Read More

View Article and Full-Text PDF

The domain of unknown function 4005 (DUF4005) in an Arabidopsis IQD protein functions in microtubule binding.

J Biol Chem 2021 May 28:100849. Epub 2021 May 28.

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. Electronic address:

The dynamic responses of microtubules (MTs) to internal and external signals are modulated by a plethora of microtubule associated proteins (MAPs). In higher plants, many plant-specific MAPs have emerged during evolution as advantageous to their sessile lifestyle. Some members of the IQ67 domain (IQD) protein family have been shown to be plant-specific MAPs. Read More

View Article and Full-Text PDF

A dual-specificity phosphatase, MAP kinase phosphatase 1, positively regulates blue light-mediated seedling development in Arabidopsis.

Planta 2021 May 31;253(6):131. Epub 2021 May 31.

National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India.

Main Conclusion: A dual-specificity phosphatase MKP1 negatively regulates the activity of MPK6 by dephosphorylating it and acts as a positive regulator of blue light (BL)-mediated photomorphogenic development in Arabidopsis. Reversible phosphorylation of proteins is one of the major post-translational modifications in nearly all signaling pathways in plants. MAP kinase phosphatases are very crucial in the regulation of MAPKs as they dephosphorylate both threonine (Thr) and tyrosine (Tyr) residues within the T-X-Y motif of active MAPKs. Read More

View Article and Full-Text PDF

Aerobic fitness is inversely associated with neurohemodynamic transduction and blood pressure variability in older adults.

Geroscience 2021 May 31. Epub 2021 May 31.

Autonomic Cardiovascular Control and Exercise Laboratory, Division of Kinesiology, School of Health and Human Performance, Faculty of Health, Dalhousie University, 6230 South Street, Halifax, NS, B3H 4R2, Canada.

Higher aerobic fitness is independently associated with better cardiovascular health in older adults. The transduction of muscle sympathetic nerve activity (MSNA) into mean arterial pressure (MAP) responses provides important insight regarding beat-by-beat neural circulatory control. Aerobic fitness is negatively associated with peak MAP responses to spontaneous MSNA in young males. Read More

View Article and Full-Text PDF

The Clinical Significance of Fluctuations in the Minute-to-minute Urine Flow Rate and in its Minute-to-minute Variability During Septic Events in Critically Ill Patients.

Rom J Anaesth Intensive Care 2020 Dec 31;27(2):1-5. Epub 2020 Dec 31.

Department of Anesthesiology and Critical Care, General Intensive Care Unit, Soroka Medical Center, Ben-Gurion University of the Negev, Beer Sheva, Israel.

Background: Septic events complicated by hemodynamic instability can lead to decreased organ perfusion, multiple organ failure, and even death. Acute renal failure is a common complication of sepsis, affecting up to 50-70 % of cases, and it is routinely diagnosed by close monitoring of urine output. We postulated that analysis of the minute-to-minute changes in the urine flow rate (UFR) and also of the changes in its minute-to-minute variability might lead to earlier diagnosis of renal failure. Read More

View Article and Full-Text PDF
December 2020

Complete response of brainstem metastasis in BRAF-mutated melanoma without stereotactic radiosurgery after initiation of encorafenib and binimetinib.

Melanoma Res 2021 May 28. Epub 2021 May 28.

Radiation Oncology, Rutgers Cancer Institute of New Jersey Neurosurgery, Rutgers University, Robert Wood Johnson Medical School, New Brunswick Medical Oncology, Perlmutter Cancer Center of New York University Langone Health/ NYU Grossman School of Medicine, New York, USA.

Metastatic melanoma is often accompanied by the development of brain metastases, at presentation or during the course of therapy. Local therapies such as surgery and radiation have been considered standard treatments for intracranial disease. However, the emergence of systemic therapies has been changing the treatment paradigm for the management of brain metastases. Read More

View Article and Full-Text PDF

Cardiorespiratory plasticity in humans following two patterns of acute intermittent hypoxia.

Exp Physiol 2021 May 28. Epub 2021 May 28.

Centre for Heart, Lung and Vascular Health, School of Health and Exercise Sciences, University of British Columbia, Kelowna, British Columbia, Canada.

New Findings: What is the central question of this study? Do cardiorespiratory experience-dependent effects (EDEs) differ between two different stimulus durations of acute isocapnic intermittent hypoxia (IHx; 5-min vs. 90-s cycles between hypoxia and normoxia)? What is the main finding and its importance? There was long-term facilitation in ventilation and blood pressure in both IHx protocols, but there was no evidence of progressive augmentation or post-hypoxia frequency decline. Not all EDEs described in animal models translate to acute isocapnic IHx responses in humans, and cardiorespiratory responses to 5-min versus 90-s on/off IHx protocols are largely similar. Read More

View Article and Full-Text PDF

Extensive divergence of projections to the forebrain from neurons in the paraventricular nucleus of the thalamus.

Brain Struct Funct 2021 Jul 25;226(6):1779-1802. Epub 2021 May 25.

Department of Oral Biology, Dr. Gerald Niznick College of Dentistry, Rady Faculty of Health Sciences, University of Manitoba, 780 Bannatyne Avenue, Winnipeg, MB, R3E 0W2, Canada.

Neurons in the paraventricular nucleus of the thalamus (PVT) respond to emotionally salient events and project densely to subcortical regions known to mediate adaptive behavioral responses. The areas of the forebrain most densely innervated by the PVT include striatal-like subcortical regions that consist of the shell of the nucleus accumbens (NAcSh), the dorsolateral region of the bed nucleus of the stria terminalis (BSTDL) and the lateral-capsular division of the central nucleus of the amygdala (CeL). A recent tracing experiment demonstrated that the PVT is composed of two intermixed populations of neurons that primarily project to either the dorsomedial (dmNAcSh) or ventromedial region of the NAcSh (vmNAcSh) with many of the vmNAcSh projecting neurons providing collateral innervation of the BSTDL and CeL. Read More

View Article and Full-Text PDF