1,617 results match your criteria respective donors

High-throughput sequencing defines donor and recipient HLA B-cell epitope frequencies for prospective matching in transplantation.

Commun Biol 2021 May 14;4(1):583. Epub 2021 May 14.

Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.

Compatibility for human leukocyte antigen (HLA) genes between transplant donors and recipients improves graft survival but prospective matching is rarely performed due to the vast heterogeneity of this gene complex. To reduce complexity, we have combined next-generation sequencing and in silico mapping to determine transplant population frequencies and matching probabilities of 150 antibody-binding eplets across all 11 classical HLA genes in 2000 ethnically heterogeneous renal patients and donors. We show that eplets are more common and uniformly distributed between donors and recipients than the respective HLA isoforms. Read More

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Quantitative magnetic resonance imaging of meniscal pathology ex vivo.

Skeletal Radiol 2021 May 13. Epub 2021 May 13.

Radiology Service, Veterans Affairs San Diego Healthcare System, MC-114, 3350 La Jolla Village Drive, San Diego, CA, 92161, USA.

Objective: To determine the ability of conventional spin echo (SE) T2 and ultrashort echo time (UTE) T2* relaxation times to characterize pathology in cadaveric meniscus samples.

Materials And Methods: From 10 human donors, 54 triangular (radially cut) meniscus samples were harvested. Meniscal pathology was classified as normal (n = 17), intrasubstance degenerated (n = 33), or torn (n = 4) using a modified arthroscopic grading system. Read More

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A non-invasive diagnostic approach to retrospective donor HLA-typing in kidney transplant patients using urine.

Transpl Int 2021 Apr 27. Epub 2021 Apr 27.

Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Internal Medicine 4 -Nephrology and Hypertension, University Hospital Erlangen, Erlangen, Germany.

Antibody mediated rejection (AMR) is a major obstacle to long-term kidney transplantation. AMR is mostly caused by donor specific HLA antibodies, which can arise before or any time after transplantation. Incomplete donor HLA-typing and unavailability of donor DNA regularly preclude the assessment of donor-specificity of circulating anti-HLA antibodies. Read More

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Combinations of self-reported rhinitis, conjunctivitis, and asthma predicts IgE sensitization in more than 25,000 Danes.

Clin Transl Allergy 2021 Mar;11(1):e12013

Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark.

Background: Allergic rhinitis (AR), allergic conjunctivitis (AC), and asthma composing multiple phenotypes and improved understanding of these phenotypes and their respective risk factors are needed.

Objectives: The objective of this study was to define the prevalence of AR, AC, and asthma and their association with allergen-specific immunoglobulin E (sIgE) sensitization in a large cohort of blood donors and identify risk factors.

Methods: From the nationwide population-based Danish Blood Donor Study, 52,976 participants completed an electronic questionnaire including AR, AC, asthma, allergic predisposition, and childhood residence. Read More

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Effect of Utilizing More Than 20-Year Older Deceased Donor Kidneys for Young Recipients: An Analysis of the UK Registry.

Exp Clin Transplant 2021 Apr 16. Epub 2021 Apr 16.

From the Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland, USA.

Objectives: Despite the wider acceptance of expanded criteria kidneys and the advances in immunosuppression, clinicians remain sceptical when it comes to accepting kidneys from significantly older donors, especially for the young adult recipient population (age ≤40 years).

Materials And Methods: We utilized prospectively maintained data from the United Kingdom Registry and analyzed the deceased donor renal transplant outcomes for 2 cohorts: (1) young recipients who received either a younger kidney or a kidney from a donor who was less than 20 years older (group <20; n = 2072) and (2) young recipients who received a kidney from donors who were 20 or more years older (group ≥20, n = 764). We used life tables for survival and performed Cox regression analysis to identify significant variables. Read More

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More Human BM-MSC With Similar Subpopulation Composition and Functional Characteristics Can Be Produced With a GMP-Compatible Fabric Filter System Compared to Density Gradient Technique.

Front Cell Dev Biol 2021 29;9:638798. Epub 2021 Mar 29.

Institute for Transfusion Medicine and Immunohematology, Goethe University Hospital, German Red Cross Blood Service Baden-Württemberg-Hessen gGmbH, Frankfurt am Main, Germany.

Background: Mesenchymal stromal cells (MSCs), multipotent progenitors that can be isolated from a variety of different tissues, are becoming increasingly important as cell therapeutics targeting immunopathologies and tissue regeneration. Current protocols for MSC isolation from bone marrow (BM) rely on density gradient centrifugation (DGC), and the production of sufficient MSC doses is a critical factor for conducting clinical MSC trials. Previously, a Good Manufacturing Practice (GMP)-compatible non-woven fabric filter device system to isolate MSCs was developed to increase the MSC yield from the BM. Read More

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Donor-recipient predicted heart mass ratio and right ventricular-pulmonary arterial coupling in heart transplant.

Eur J Cardiothorac Surg 2021 Apr;59(4):847-854

Graduate Studies Program on Cardiovascular Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.


Objectives: Right ventricular-pulmonary arterial (RV-PA) coupling interactions are largely unexplored in heart transplant patients. The outcome of this study was RV-PA coupling at 7 and 30 days after heart transplant and its association with donor-recipient size matching.

Methods: Clinical, echocardiographic and haemodynamic data from a retrospective cohort of heart transplant recipients and respective donors were reviewed. Read More

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Seroprevalence and trends of transfusion transmissible infections among retrospective blood donors in Western Azerbaijan Regional Blood Transfusion Center, Iran: A ten-years evaluation.

Transfus Apher Sci 2021 Apr 3:103132. Epub 2021 Apr 3.

Azarbaijan-Gharbi Regional Blood Transfusion Center, Urmia, Iran; Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address:

Transfusion transmissible infections (TTIs) have been a public health challenge for the accessibility, quality and safety of blood transfusion. The present study aimed to consider the prevalence and the trends of hepatitis B virus (HBV), hepatitis C virus (HCV), Human T-cell leukemia virus type 1 (HTLV-1), human immunodeficiency virus (HIV) and syphilis across the ten years among retrospective blood donors. A retrospective investigation of blood donors' data covering the period from 22 May 2009 to 22 May 2019 was done. Read More

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BAY 60-6583 Enhances the Antitumor Function of Chimeric Antigen Receptor-Modified T Cells Independent of the Adenosine A2b Receptor.

Front Pharmacol 2021 12;12:619800. Epub 2021 Mar 12.

Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.

Chimeric antigen receptor (CAR) T cells are powerful in eradicating hematological malignancies, but their efficacy is limited in treating solid tumors. One of the barriers is the immunosuppressive response induced by immunomodulatory signaling pathways. Pharmacological targeting of these immunosuppressive pathways may be a simple way to improve the efficacy of CAR T cells. Read More

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Development of a CD8 co-receptor independent T-cell receptor specific for tumor-associated antigen MAGE-A4 for next generation T-cell-based immunotherapy.

J Immunother Cancer 2021 Mar;9(3)

Medigene Immunotherapies GmbH, Planegg-Martinsried, Germany.

Background: The cancer-testis antigen MAGE-A4 is an attractive target for T-cell-based immunotherapy, especially for indications with unmet clinical need like non-small cell lung or triple-negative breast cancer.

Methods: An unbiased CD137-based sorting approach was first used to identify an immunogenic MAGE-A4-derived epitope (GVYDGREHTV) that was properly processed and presented on human leukocyte antigen (HLA)-A2 molecules encoded by the HLA-A*02:01 allele. To isolate high-avidity T cells via subsequent multimer sorting, an in vitro priming approach using HLA-A2-negative donors was conducted to bypass central tolerance to this self-antigen. Read More

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Establishment of Hematological Reference Values among Healthy Adults in Bamenda, North West Region of Cameroon.

Anemia 2021 24;2021:6690926. Epub 2021 Feb 24.

The University of Bamenda, Faculty of Health Sciences, Department of Medical Laboratory Science, P.O. Box 39 Bambili, Bamenda, North West Region, Cameroon.

The use of the reference range of values of a laboratory test is highly significant in diagnostic accuracy. However, race and ethnic variations may affect the safe use of reference ranges from a different setting/population. Because the establishment of reference ranges for the Cameroonian population will possibly improve the quality of health care, this study was designed to establish hematological reference ranges among healthy adults in Bamenda, North West region of Cameroon. Read More

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February 2021

Soluble factors differ in platelets derived from separate niches: a pilot study comparing the secretome of peripheral blood and bone marrow platelets.

Cytotherapy 2021 Mar 5. Epub 2021 Mar 5.

Elite Regenerative Stem Cell Specialists, LLC, Johnstown, Colorado, USA; R&D Regenerative Laboratory Resources, LLC, Johnstown, Colorado, USA; Colorado Spine Institute, PLLC, Johnstown, Colorado, USA.

Background Aims: Platelet-rich plasma (PRP) and bone marrow aspirate are commonly used in orthobiologics for their anti-inflammatory, anabolic/regenerative and immunomodulatory characteristics via platelet degranulation and cell secretions. Although platelets are derived from megakaryocytes in the bone marrow, no attention has been paid to the potential benefits of bone marrow platelets and whether their contents differ from aging platelets in peripheral blood.

Methods: In the present study, leukocyte-poor peripheral blood-derived platelets in plasma (LPP) and leukocyte-poor bone marrow platelets in plasma (BMP) were prepared from six donors, activated with calcium chloride, incubated and sampled at day 0, day 3 and day 6. Read More

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Improving Liver Graft Function Using CD47 Blockade in the Setting of Normothermic Machine Perfusion.

Transplantation 2021 Feb 11. Epub 2021 Feb 11.

Department of Surgery, Washington University School of Medicine, St. Louis, MO, USA Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA Arch Oncology, Inc., St. Louis, MO, USA.

Background: Towards the goal of utilizing more livers for transplantation, transplant centers are looking to increase the use of organs from "marginal" donors. Livers from these donors, however, have been shown to be more susceptible to preservation and reperfusion injury.

Methods: Using a porcine model of donation after circulatory death (DCD), we studied the use of antibody-mediated CD47 blockade to further improve liver graft function undergoing normothermic machine perfusion. Read More

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February 2021

Rapid and noninvasive diagnosis of oral and oropharyngeal cancer based on micro-Raman and FT-IR spectra of saliva.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 18;252:119477. Epub 2021 Jan 18.

Biomolecular Physics Department, Faculty of Physics, Babes-Bolyai University, Cluj-Napoca, Romania. Electronic address:

Fast, sensitive, and noninvasive techniques are needed for better health care management, particularly when traditional biopsies could be replaced with appropriate analyses of body fluids, such as saliva. Here is presented a proof-of-concept study, which aims to test a recently developed saliva samples preparation method, for oral and oropharyngeal cancer diagnosis, using micro-Raman and Fourier transform infrared (FT-IR) spectroscopic techniques. The detected biomarker bands and the cancer classification rates are compared and discussed. Read More

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Catecholaminergic Vasopressors Reduce Toll-Like Receptor Agonist-Induced Microvascular Endothelial Cell Permeability But Not Cytokine Production.

Crit Care Med 2021 03;49(3):e315-e326

Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA.

Objectives: Catecholaminergic vasopressors are the cornerstone of circulatory shock management. Nevertheless, catecholamines have problematic side effects, arousing a growing interest in noncatecholaminergic agents such as vasopressin or angiotensin-II. However, their respective effects on sepsis-associated microvascular endothelial dysfunction such as permeability or inflammation remain elusive. Read More

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Peripheral blood mononuclear cells preferentially activate 11-oxygenated androgens.

Eur J Endocrinol 2021 Mar;184(3):353-363

Institute of Metabolism and Systems Research, University of Birmingham, Birmingham, UK.

Objective: Androgens are important modulators of immune cell function. The local generation of active androgens from circulating precursors is an important mediator of androgen action in peripheral target cells or tissues. We aimed to characterize the activation of classic and 11-oxygenated androgens in human peripheral blood mononuclear cells (PBMCs). Read More

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MALDI TOF Mass Spectrometry Imaging of Blood Smear: Method Development and Evaluation.

Int J Mol Sci 2021 Jan 8;22(2). Epub 2021 Jan 8.

Clinical Institute of Laboratory Diagnostics, Osijek University Hospital, J. Huttlera 4, 31 000 Osijek, Croatia.

The aim of this study was to develop and evaluate matrix assisted LASER desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry imaging (MSI) of blood smear. Integrated light microscope and MALDI IT-TOF mass spectrometer, together with a matrix sublimation device, were used for analysis of blood smears coming from healthy male donors. Different blood plasma removal, matrix deposition, and instrumental settings were evaluated using the negative and positive ionization modes while agreement between the light microscopy images and the lateral distributions of cellular marker compounds served as the MSI quality indicator. Read More

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January 2021

The Origins of Salivary Vitamin A, Vitamin B and Vitamin D-Binding Proteins.

Nutrients 2020 Dec 16;12(12). Epub 2020 Dec 16.

Salivary Research, Centre for Host-Microbiome Interactions, Faculty of Dental, Oral and Craniofacial Sciences (FoDOCS), King's College London, London SE1 1UL, UK.

Vitamin A- (retinol), vitamin B- (haptocorrin) and vitamin D-binding proteins are the major circulatory transporters of their respective ligands; they are also constituents of the salivary proteome, the origins of which, remain unclear. The aim of this study was to explore how these proteins enter saliva and their relationship (if any) with vitamin status. Firstly, the three vitamin-binding proteins were quantified in resting whole mouth saliva and chewing-stimulated saliva from healthy donors ( = 10) to determine if they enter the mouth by salivary secretion or from the circulation. Read More

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December 2020

Immune Status Against Hepatitis B in Patients After Allogeneic Hematopoietic Cell Transplantation-Factors Affecting Early and Long-Lasting Maintenance of Protective Anti-HBs Titers.

Front Immunol 2020 24;11:586523. Epub 2020 Nov 24.

Department of Hematology and Transplantology, Medical University of Gdansk, Gdansk, Poland.

The immunization of allogeneic hematopoietic cell transplantation (HCT) recipients against vaccine-preventable diseases is a part of posttransplantation guidelines. We conducted a prospective study to assess clinical and immunological parameters that would determine the response and long-term maintenance of protective antibody titers upon the hepatitis B virus (HBV) vaccination after HCT. The investigated variables included: vaccination of the HCT recipients and their donors prior to HCT, chronic graft versus host disease (cGVHD) and the timing of post-HCT vaccination, and B- and T-cell subtype status. Read More

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November 2020

Maturation of Monocyte-Derived DCs Leads to Increased Cellular Stiffness, Higher Membrane Fluidity, and Changed Lipid Composition.

Front Immunol 2020 27;11:590121. Epub 2020 Nov 27.

Laboratory of Dendritic Cell Biology, Department of Dermatology, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University Hospital Erlangen, Erlangen, Germany.

Dendritic cells (DCs) are professional antigen-presenting cells of the immune system. Upon sensing pathogenic material in their environment, DCs start to mature, which includes cellular processes, such as antigen uptake, processing and presentation, as well as upregulation of costimulatory molecules and cytokine secretion. During maturation, DCs detach from peripheral tissues, migrate to the nearest lymph node, and find their way into the correct position in the net of the lymph node microenvironment to meet and interact with the respective T cells. Read More

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November 2020

The applicability of forensic time since death estimation methods for buried bodies in advanced decomposition stages.

PLoS One 2020 9;15(12):e0243395. Epub 2020 Dec 9.

Institute of Legal Medicine, Goethe-University Frankfurt, Frankfurt, Germany.

Estimation of the postmortem interval in advanced postmortem stages is a challenging task. Although there are several approaches available for addressing postmortem changes of a (human) body or its environment (ecologically and/or biochemically), most are restricted to specific timeframes and/or individual and environmental conditions. It is well known, for instance, that buried bodies decompose in a remarkably different manner than on the ground surface. Read More

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January 2021

Psychosocial risk factors for impaired health-related quality of life in living kidney donors: results from the ELIPSY prospective study.

Sci Rep 2020 12 7;10(1):21343. Epub 2020 Dec 7.

Medical School, University of Barcelona, Barcelona, Spain.

Living kidney donors' follow-up is usually focused on the assessment of the surgical and medical outcomes. Whilst the psychosocial follow-up is advocated in literature. It is still not entirely clear which exact psychosocial factors are related to a poor psychosocial outcome of donors. Read More

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December 2020

[Analysis on Polymorphism of Platelet Antigen Gene in Shandong Han Population].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Dec;28(6):2066-2070

HLA Laboratory, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China,E-mail:

Objective: To study the Polymorphism of the human platelet antigen(HPA) gene 1-17 and human leukocyte antigen(HLA) gene-A and B locus in Shandong Han population.

Methods: A total of 962 samples from routine voluntary platelet donors were genotyped for HPA1-17 system and HLA-A site, B by PCR-SSP and PCR-SSOP respectively.Gene frequencies were calculated by counting. HPA1-17 and HLA genotype combinations were analyzed by Arelequin 3. Read More

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December 2020

A Europe wide acceptable mismatch program will enable transplantation of long waiting highly sensitised patients with a compatible donor.

Transpl Immunol 2021 02 1;64:101354. Epub 2020 Dec 1.

Eurotransplant Reference Laboratory, Leiden University Medical Centre, Leiden, the Netherlands. Electronic address:

Immunisation against Human Leucocyte Antigens (HLA) can be caused by pregnancy, blood transfusion, or organ transplants. The HLA antibody status of a given patient significantly influences their access and waiting time to transplant. For some highly sensitised patients (HSP) there is hardly any suitable donor available in the deceased donor pool of their allocation organisation and therefore they wait a very long time before being offered a kidney for transplant. Read More

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February 2021

Outcomes of Highly Selected Live Donors With a Future Liver Remnant Less Than or Equal to 30%: A Matched-Cohort Study.

Transplantation 2020 Nov 24. Epub 2020 Nov 24.

Multi-Organ Transplant Unit, Toronto General Hospital, University of Toronto, Toronto, Canada.

Background: The main concern with live donor liver transplantation (LDLT) is the risk to the donor. Given the potential risk of liver insufficiency, most centres will only accept candidates with future liver remnants (FLR) >30%. We aimed to compare postoperative outcomes of donors who underwent LDLT with FLR ≤30% and >30%. Read More

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November 2020

Chronic Active Antibody-Mediated Rejection with Linear IgG Deposition on Glomerular Capillaries in a Kidney Transplant Recipient.

Nephron 2020 25;144 Suppl 1:97-101. Epub 2020 Nov 25.

Department of Pediatric Nephrology, Tokyo Women's Medical University, Tokyo, Japan,

Glomerular IgG deposition is rarely observed in antibody-mediated rejection. Here, we report chronic active antibody-mediated rejection with linear IgG deposition on glomerular capillary walls in a pediatric kidney transplant recipient. A 6-year-old boy with bilateral renal hypoplasia underwent preemptive deceased-donor kidney transplantation. Read More

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November 2020

Deep phenotypical characterization of human CD3 CD56 T cells by mass cytometry.

Eur J Immunol 2021 Mar 15;51(3):672-681. Epub 2020 Dec 15.

Institute for Medical Microbiology and Hospital Hygiene, University of Marburg, Marburg, Germany.

CD56 T cells are a group of pro-inflammatory CD3 lymphocytes with characteristics of natural killer cells, being involved in antimicrobial immune defense. Here, we performed deep phenotypic profiling of CD3 CD56 cells in peripheral blood of normal human donors and individuals sensitized to birch-pollen or/and house dust mite by high-dimensional mass cytometry combined with manual and computational data analysis. A co-regulation between major conventional T-cell subsets and their respective CD3 CD56 cell counterparts appeared restricted to CD8 , MAIT, and TCRγδ T-cell compartments. Read More

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Multi National Survey of the Advice Given to Muslim Kidney Graft Recipients by Muslim Nephrologists about Lifestyle and Religious Rituals with Potential Medical Risk.

Saudi J Kidney Dis Transpl 2020 Sep-Oct;31(5):957-981

Adult Transplant Nephrology, Department of Organ Transplant Center, King of Abdulaziz Medical City, King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences; Riyadh, Saudi Arabia.

Muslim renal transplant recipients often ask their physicians if performing certain lifestyles or religious obligations may be harmful to their health. Permissibility as advised by an expert Muslim physician is considered as being religiously accepted. A cross-sectional, survey-based study was conducted enquiring what nephrologists would advise their transplant recipients to do, about some lifestyles and religious duties. Read More

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November 2020

A streamlined proliferation assay using mixed lymphocytes for evaluation of human mesenchymal stem cell immunomodulation activity.

J Immunol Methods 2021 Jan 16;488:112915. Epub 2020 Nov 16.

Blood and Coagulation Research, US Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, United States of America.

Background: Mesenchymal stromal cells (MSCs) have been proposed for treatment of acute respiratory distress syndrome (ARDS), graft versus host disease (GVHD), wound healing and trauma. A consensus is building that immunomodulation by MSCs is important for therapeutic potential. MSCs suppress peripheral blood mononuclear cell (PBMC) proliferation in vitro, potentially reflecting an ability to suppress PBMC inflammatory responses in vivo. Read More

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January 2021

Steroid precursors, steroids, neuroactive steroids, and neurosteroids concentrations in serum and saliva of healthy neonatal heifer Holstein calves.

J Vet Intern Med 2020 Nov 17;34(6):2767-2775. Epub 2020 Nov 17.

Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, USA.

Background: Persistence of high neurosteroid concentrations in blood is associated with neonatal encephalopathy and septicemia in foals. This has not been investigated in calves.

Objectives: To determine concentrations of steroid compounds in serum and saliva within the first 48 hours after birth in healthy neonatal calves, identify potential markers for disease, and investigate the association between serum steroid compounds concentrations in calves and their respective dams within 2 hours after birth. Read More

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November 2020