35,359 results match your criteria resonance vitro


Alkaloid derivative ION-31a inhibits breast cancer metastasis and angiogenesis by targeting HSP90α.

Bioorg Chem 2021 Jul 22;115:105201. Epub 2021 Jul 22.

School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China; Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, PR China. Electronic address:

Breast cancer has become the number one killer of women. In our previous study, an active compound, ION-31a, with potential anti-metastasis activity against breast cancer was identified through the synthesis of ionone alkaloid derivatives. In the present study, we aimed to identify the therapeutic target of ION-31a. Read More

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S-nitroso human serum albumin as a nitric oxide donor in drug-eluting vascular grafts: biofunctionality and preclinical evaluation.

Acta Biomater 2021 Jul 27. Epub 2021 Jul 27.

Center for Biomedical Research, Medical University Vienna, Austria; Ludwig Boltzmann Institute for Cardiovascular Research, Vienna, Austria; Austrian Cluster for Tissue Regeneration. Electronic address:

Currently available synthetic small diameter vascular grafts reveal low patency rates due to thrombosis and intimal hyperplasia. Biofunctionalized grafts releasing nitric oxide (NO) in situ may overcome these limitations. In this study, a drug-eluting vascular graft was designed by blending polycaprolactone (PCL) with S-nitroso-human-serum-albumin (S-NO-HSA), a nitric oxide donor with prolonged half-life. Read More

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Mechanistic studies of non-canonical amino acid mutagenesis.

Methods Enzymol 2021 24;656:375-428. Epub 2021 Jun 24.

Department of Chemistry, Columbia University, New York, NY, United States. Electronic address:

Over the past decade, harnessing the cellular protein synthesis machinery to incorporate non-canonical amino acids (ncAAs) into tailor-made peptides has significantly advanced many aspects of molecular science. More recently, groundbreaking progress in our ability to engineer this machinery for improved ncAA incorporation has led to significant enhancements of this powerful tool for biology and chemistry. By revealing the molecular basis for the poor or improved incorporation of ncAAs, mechanistic studies of ncAA incorporation by the protein synthesis machinery have tremendous potential for informing and directing such engineering efforts. Read More

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Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects.

Phytochemistry 2021 Jul 27;190:112861. Epub 2021 Jul 27.

Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100, Puławy, Poland.

The study is based on phytochemical profiling and in vitro evaluation of biological effects of phenolic acid derivatives-rich Herniaria fractions, isolated from two rupturewort (Herniaria L.) species, i.e. Read More

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Smart Design of a pH-Responsive System Based on pHLIP-Modified Magnetite Nanoparticles for Tumor MRI.

ACS Appl Mater Interfaces 2021 Jul 29. Epub 2021 Jul 29.

Postovsky Institute of Organic Synthesis, Russian Academy of Sciences (Ural Branch), 620108 Yekaterinburg, Russia.

Magnetic FeO nanoparticles (MNPs) are often used to design agents enhancing contrast in magnetic resonance imaging (MRI) that can be considered as one of the efficient methods for cancer diagnostics. At present, increasing the specificity of the MRI contrast agent accumulation in tumor tissues remains an open question and attracts the attention of a wide range of researchers. One of the modern methods for enhancing the efficiency of contrast agents is the use of molecules for tumor acidic microenvironment targeting, for example, pH-low insertion peptide (pHLIP). Read More

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MFGM components promote gut Bifidobacterium growth in infant and in vitro.

Eur J Nutr 2021 Jul 29. Epub 2021 Jul 29.

National Engineering Center of Dairy for Maternal and Child Health, Beijing Sanyuan Foods Co. Ltd., No. 8, Yingchang Street, Yinghai Town, Daxing District, Beijing, 100163, China.

Purpose: Infant gut microbiota which plays an important role in long-term health is mainly shaped by early life nutrition. However, the effect of nutrients on infants gut microbiota is less researched. Here, we present a study aiming to investigate in vitro a modified formula that is supplemented with milk fat globule membrane (MFGM) that were missing in common formulas when compared with human milk and to assess the impact of feeding scheme on microbiota and metabolism. Read More

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An MSN-based synergistic nanoplatform for root canal biofilm eradication Fenton-enhanced sonodynamic therapy.

J Mater Chem B 2021 Jul 29. Epub 2021 Jul 29.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.

The validity and biocompatibility of irrigating agents are imperative for the success of root canal therapy. The imperfections in the currently available irrigants highlight the fact that more advanced technologies and strategies are required for complete disinfection in endodontic treatments. In the present study, a Fenton reaction-enhanced antimicrobial sonodynamic therapy (SDT) platform was fabricated for root canal disinfection. Read More

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PfHRP2 detection using plasmonic optrodes: performance analysis.

Malar J 2021 Jul 28;20(1):332. Epub 2021 Jul 28.

Electromagnetism and Telecommunications Department, University of Mons, Bld. Dolez 31, 7000, Mons, Belgium.

Background: Early malaria diagnosis and its profiling require the development of new sensing platforms enabling rapid and early analysis of parasites in blood or saliva, aside the widespread rapid diagnostic tests (RDTs).

Methods: This study shows the performance of a cost-effective optical fiber-based solution to target the presence of Plasmodium falciparum histidine-rich protein 2 (PfHRP2). Unclad multimode optical fiber probes are coated with a thin gold film to excite Surface Plasmon Resonance (SPR) yielding high sensitivity to bio-interactions between targets and bioreceptors grafted on the metal surface. Read More

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Eco-friendly sunscreen formulation based on starches and PEG-75 lanolin increases the antioxidant capacity and the light scattering activity in the visible light.

J Photochem Photobiol B 2021 Jul 22;222:112264. Epub 2021 Jul 22.

Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Department of Dermatology, Venereology and Allergology, Charitéplatz 1, 10117 Berlin, Germany. Electronic address:

Most modern sunscreens contain physical filters, which scatter the sunlight, increasing the photons' pathway in the upper stratum corneum. This effect can lead to a better efficacy of the UV filters and improve the diffuse reflection. However, the addition of nanosized inorganic UV filters reduces the antioxidant capacity of sunscreen formulations. Read More

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Phase-contrast acceleration mapping with synchronized encoding.

Magn Reson Med 2021 Jul 27. Epub 2021 Jul 27.

Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Purpose: To develop a phase-contrast (PC) -based method for direct and unbiased quantification of the acceleration vector field by synchronization of the spatial and acceleration encoding time points. The proposed method explicitly aims at in-vitro applications, requiring high measurement accuracy, as well as the validation of clinically relevant acceleration-encoded sequences.

Methods: A velocity-encoded sequence with synchronized encoding (SYNC SPI) was modified to allow direct acceleration mapping by replacing the bipolar encoding gradients with tripolar gradient waveforms. Read More

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Integrated Microscopy and Metabolomics to Test an Innovative Fluid Dynamic System for Skin Explants .

Microsc Microanal 2021 Jul 27:1-12. Epub 2021 Jul 27.

Department of Neurosciences, Biomedicine and Movement Sciences, Section of Anatomy and Histology, School of Medicine and Surgery, University of Verona, Verona37134, Italy.

The in vitro models are receiving growing attention in studies on skin permeation, penetration, and irritancy, especially for the preclinical development of new transcutaneous drugs. However, synthetic membranes or cell cultures are unable to effectively mimic the permeability and absorption features of the cutaneous barrier. The use of explanted skin samples maintained in a fluid dynamic environment would make it possible for an in vitro experimentation closer to in vivo physiological conditions. Read More

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Extrusion-based 3D printing of ex situ-alloyed highly biodegradable MRI-friendly porous iron-manganese scaffolds.

Acta Biomater 2021 Jul 23. Epub 2021 Jul 23.

Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, Mekelweg 2, 2628 CD Delft, The Netherlands.

Additively manufactured biodegradable porous iron has been only very recently demonstrated. Two major limitations of such a biomaterial are very low biodegradability and incompatibility with magnetic resonance imaging (MRI). Here, we present a novel biomaterial that resolves both of those limitations. Read More

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DNAzyme Sensor Uses Chemiluminescence Resonance Energy Transfer for Rapid, Portable, and Ratiometric Detection of Metal Ions.

Anal Chem 2021 Jul 26. Epub 2021 Jul 26.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

DNAzymes have emerged as an important class of sensors for a wide variety of metal ions, with florescence DNAzyme sensors as the most widely used in different sensing and imaging applications because of their fast response time, high signal intensity, and high sensitivity. However, the requirements of an external excitation light source and its associated power increase the cost and size of the fluorometer, making it difficult to be used for portable detections. To overcome these limitations, we report herein a DNAzyme sensor that relies on chemiluminescence resonance energy transfer (CRET) without the need for external light. Read More

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Metabolic profiling, in vitro propagation, and genetic assessment of the endangered rare plant Anarrhinum pubescens.

J Genet Eng Biotechnol 2021 Jul 26;19(1):108. Epub 2021 Jul 26.

Department of Chemistry, Claflin University, Orangeburg, SC, 29115, USA.

Background: Anarrhinum pubescens Fresen. (Plantaginaceae) is a rare plant, endemic to the Saint Catherine area, of South Sinai, Egypt. Earlier studies have reported the isolation of cytotoxic and anti-cholinesterase iridoid glucosides from the aerial parts of the plant. Read More

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A rationally designed c-di-AMP FRET biosensor to monitor nucleotide dynamics.

J Bacteriol 2021 Jul 26:JB0008021. Epub 2021 Jul 26.

Department of Microbiology, University of Washington, Seattle, WA, 98195, USA.

3'3'-cyclic di-adenosine monophosphate (c-di-AMP) is an important nucleotide second messenger found throughout the bacterial domain of life. C-di-AMP is essential in many bacteria and regulates a diverse array of effector proteins controlling pathogenesis, cell wall homeostasis, osmoregulation, and central metabolism. Despite the ubiquity and importance of c-di-AMP, methods to detect this signaling molecule are limited, particularly at single cell resolution. Read More

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Retinoic acid-conjugated chitosan/manganese porphyrin ionic-complex nanoparticles for improved T contrast MR imaging of hepatic fibrosis.

J Biomed Mater Res B Appl Biomater 2021 Jul 26. Epub 2021 Jul 26.

Department of Biomedical Science, BK21 FOUR Program in Biomedical Science and Engineering, Inha University College of Medicine, Incheon, South Korea.

Noninvasive and precise diagnosis of hepatic fibrosis is very important for the preventive therapeutic regimen of hepatic cirrhosis and cancer. In this study, we fabricated T contrast Mn-porphyrin (MnTPPS )/retinoic acid-chitosan ionic-complex nanoparticles (MRC NPs). The functional properties of MRC NPs were evaluated via transmission electron microscopy (TEM) imaging, release study, cytotoxicity assay, hepatocyte-specific uptake assay, and magnetic resonance (MR) imaging study. Read More

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Intranasal Paclitaxel Alters Alzheimer's Disease Phenotypic Features in 3xTg-AD Mice.

J Alzheimers Dis 2021 Jul 23. Epub 2021 Jul 23.

Department of Radiology and Imaging Sciences>, University of Utah, Salt Lake City, UT, USA.

Background: Microtubule stabilizing drugs, commonly used as anti-cancer therapeutics, have been proposed for treatment of Alzheimer's disease (AD); however, many do not cross the blood-brain barrier.

Objective: This research investigated if paclitaxel (PTX) delivered via the intranasal (IN) route could alter the phenotypic progression of AD in 3xTg-AD mice.

Methods: We administered intranasal PTX in 3XTg-AD mice (3xTg-AD n = 15, 10 weeks and n = 10, 44 weeks, PTX: 0. Read More

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Ferrous ions doped layered double hydroxide: smart 2D nanotheranostic platform with imaging-guided synergistic chemo/photothermal therapy for breast cancer.

Biomater Sci 2021 Jul 26. Epub 2021 Jul 26.

Key Laboratory of Advanced Materials, Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China and State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

Developing simple and efficient nanotheranostic platforms with behavior responsive to the acid microenvironment of a tumor is of great significance for accurate tumor diagnosis and therapy. In this study, a smart 2D nanotheranostic platform has been successfully fabricated by doping functional ferrous ions into as-synthesized MgAl-layered double hydroxide (LDH) with doxurubicin (DOX) loading to form Fe-LDH/DOX NPs, which achieved magnetic resonance imaging (MRI)-guided synergistic chemo/photothermal therapy for breast cancer. The doping of ferrous ions into Fe-LDH/DOX enabled a strong photo-induced heating ability with a high photothermal conversion efficiency of 45. Read More

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Lunate bone loss associated with infection.

Trauma Case Rep 2021 Aug 18;34:100431. Epub 2021 Feb 18.

Department of Hand surgery and Microsurgery, University Hospital of Modena, Italy.

Respiratory pathogens such as may activate osteoclast cells, thereby inducing bone resorption and joint inflammation. Herein is a case report of a young man with misdiagnosed persistent wrist pain without any major trauma. Investigation of the patient's medical history confirmed a recent systemic infection. Read More

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Cavity-Containing [FeL] Helicates: An Examination of Host-Guest Chemistry and Cytotoxicity.

Front Chem 2021 7;9:697684. Epub 2021 Jul 7.

Department of Chemistry, University of Otago, Dunedin, New Zealand.

Two new di(2,2'-bipyridine) ligands, 2,6-bis([2,2'-bipyridin]-5-ylethynyl)pyridine () and bis(4-([2,2'-bipyridin]-5-ylethynyl)phenyl)methane () were synthesized and used to generate two metallosupramolecular [Fe()](BF) cylinders. The ligands and cylinders were characterized using elemental analysis, electrospray ionization mass spectrometry, UV-vis, H-, C and DOSY nuclear magnetic resonance (NMR) spectroscopies. The molecular structures of the [Fe()](BF) cylinders were confirmed using X-ray crystallography. Read More

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Citrate-Coated Magnetic Polyethyleneimine Composites for Plasmid DNA Delivery into Glioblastoma.

Polymers (Basel) 2021 Jul 6;13(14). Epub 2021 Jul 6.

School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.

Several ternary composites that are based on branched polyethyleneimine (bPEI 25 kDa, polydispersity 2.5, 0.1 or 0. Read More

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Effects of Gadolinium Deposits in the Cerebellum: Reviewing the Literature from In Vitro Laboratory Studies to In Vivo Human Investigations.

Int J Environ Res Public Health 2021 Jul 6;18(14). Epub 2021 Jul 6.

Department of Integrative Physiology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.

Gadolinium (Gd)-based contrast agents (GBCAs) are chemicals injected intravenously during magnetic resonance imaging (MRI) to enhance the diagnostic yield. The repeated use of GBCAs can cause their deposition in the brain, including the cerebellum. Such deposition may affect various cell subsets in the brain and consequently cause behavioral alterations due to neurotoxicity. Read More

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Bioguided Isolation of Active Compounds from against Methicillin-Resistant (MRSA) and Panton-Valentine Leucocidin Positive Strains (MSSA-PVL).

Molecules 2021 Jul 19;26(14). Epub 2021 Jul 19.

Laboratory of Microbial Biotechnology and Bioactive Molecules, Department of Biology, Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez 2202, Morocco.

Despite intensified efforts to develop an effective antibiotic, is still a major cause of mortality and morbidity worldwide. The multidrug resistance of bacteria has considerably increased the difficulties of scientific research and the concomitant emergence of resistance is to be expected. In this study we have investigated the in vitro activity of 15 ethanol extracts prepared from Moroccan medicinal plants traditionally used for treatment of skin infections. Read More

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Photoactivatable Red Chemiluminescent AIEgen Probe for / Imaging Assay of Hydrazine.

Anal Chem 2021 Jul 23. Epub 2021 Jul 23.

State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.

Here, we have developed a novel photoactivatable red chemiluminescent AIEgen probe (ACL), based on the combination of the red-emission AIEgen fluorophore (TPEDC) that shows excellent singlet oxygen (O)-generation ability and the precursor of Schaap's dioxetane (the linker connected to adamantane is the C═C bond) that can be modified to target various analytes, for and measurement of hydrazine. Prior to applying for sensing detection, the C═C bond connected to adamantane in ACL was first converted into dioxetane by irradiation to form the activated chemiluminescent AIEgen probe (ACLD). Then, the self-immolative reaction was triggered upon the deprotection of the acylated phenolic hydroxyl group in ACLD in the presence of hydrazine, resulting in the release of the high energy held in the 1,2-dioxetanes, and then, the chemiexcitation was triggered, thereby producing red chemiluminescence through the intramolecular chemiluminescence resonance energy transfer from Schaap's dioxetane to TPEDC. Read More

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Taxifolin, an Inhibitor of Sortase A, Interferes With the Adhesion of Methicillin-Resistant .

Front Microbiol 2021 6;12:686864. Epub 2021 Jul 6.

College of Animal Science, Jilin University, Changchun, China.

The evolution and spread of methicillin-resistant (MRSA) poses a significant hidden risk to human public health. The majority of antibiotics used clinically have become mostly ineffective, and so the development of novel anti-infection strategies is urgently required. Since () cysteine transpeptidase sortase A (SrtA) mediates the surface-anchoring of proteins to its surface, compounds that inhibit SrtA are considered potential antivirulence treatments. Read More

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Design and evaluation of GLP-1 receptor G-protein biased agonist with prolonged efficacy on diabetes.

Life Sci 2021 Jul 19:119844. Epub 2021 Jul 19.

Peking University Third Hospital, Beijing 100191, PR China. Electronic address:

Aim: Glucagon-like peptide-1 receptor agonists (GLP-1RAs) have emerged as potent drug development target for treating diabetes and obesity. Here, we designed and evaluated novel long-acting GLP-1R G protein biased agonist with potent clinical application.

Main Methods: GLP-1R G-protein biased sequences were screened via a high-throughput autocrine-based strategy and then fused to Exendin (9-39). Read More

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Anisotropic Truncated Octahedral Au with Pt Deposition on Arris for Localized Surface Plasmon Resonance-Enhanced Photothermal and Photodynamic Therapy of Osteosarcoma.

ACS Appl Mater Interfaces 2021 Jul 22. Epub 2021 Jul 22.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221002, People's Republic of China.

The multifunctional combined nanoplatform has a wide application prospect in the synergistic treatment of cancer. Nevertheless, the traditional treatment of phototherapy is limited by the catalytic nanomaterial itself, so the effect is not satisfactory. Here, the arris of the anisotropic truncated octahedral Au (TOh Au) was coated with noble metal Pt to form a spatial separation structure, which enhanced the local surface plasmonic resonance and thus boosted the photocatalytic effect. Read More

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synthesis of fluorescent polydopamine on biogenic MnO nanoparticles as stimuli responsive multifunctional theranostics.

Biomater Sci 2021 Jul 21. Epub 2021 Jul 21.

School of Chemistry and Chemical Engineering, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, Huazhong University of Science and Technology, Wuhan 430074, China.

Multifunctional nanocomposites have drawn great attention in clinical applications because of their ability to integrate diagnostic and therapeutic functions. Manganese dioxide (MnO2), owing to its biocompatibility and magnetic resonance imaging (MRI) properties, has been widely applied in biomedical research. Our previous work on biogenic MnO2 nanoparticles (Bio-MnO2 NPs) revealed that intrinsic photothermal properties and stimuli-responsive MRI imaging are particularly promising for the development of theranostic systems. Read More

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Combining Well-Tempered Metadynamics Simulation and SPR Assays to Characterize the Binding Mechanism of the Universal T-Lymphocyte Tetanus Toxin Epitope TT830-843.

Biomed Res Int 2021 4;2021:5568980. Epub 2021 Jul 4.

Centro de Desenvolvimento Tecnológico em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.

Peptide TT830-843 from the tetanus toxin is a universal T-cell epitope. It helps in vaccination and induces T-cell activation. However, the fine molecular interaction between this antigen and the major histocompatibility complex (MHC) remains unknown. Read More

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Physiologically Based Pharmacokinetic Modeling of Transporter-Mediated Hepatic Disposition of Imaging Biomarker Gadoxetate in Rats.

Mol Pharm 2021 Aug 20;18(8):2997-3009. Epub 2021 Jul 20.

Centre for Applied Pharmacokinetic Research, School of Health Sciences, University of Manchester, Manchester M13 9PL, U.K.

Physiologically based pharmacokinetic (PBPK) models are increasingly used in drug development to simulate changes in both systemic and tissue exposures that arise as a result of changes in enzyme and/or transporter activity. Verification of these model-based simulations of tissue exposure is challenging in the case of transporter-mediated drug-drug interactions (tDDI), in particular as these may lead to differential effects on substrate exposure in plasma and tissues/organs of interest. Gadoxetate, a promising magnetic resonance imaging (MRI) contrast agent, is a substrate of organic-anion-transporting polypeptide 1B1 (OATP1B1) and multidrug resistance-associated protein 2 (MRP2). Read More

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