421,891 results match your criteria resolution

Hemoglobin Yamagata [132(H10)Lys→Asn; (: c.399A>T)]: a mosaic to be put together.

Clin Chem Lab Med 2021 Apr 22. Epub 2021 Apr 22.

Human Genetics Laboratory, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Giannina Gaslini, Genova, Italy.

Objectives: Artifactually altered glycated hemoglobin (HbA) concentrations are frequently linked to hemoglobin (Hb) variants. Their expression and detection require in-depth analysis.

Methods: Cation exchange high performance liquid chromatography (HPLC) (Bio-Rad Variant™ II; Trinity Biotech Premier Hb9210 Resolution), capillary electrophoresis (CE) (Sebia Capillarys 2 Flex Piercing) and mass spectrometry (MS) (Waters) were used for variant detection; Sanger sequencing, multiplex ligation-dependent probe amplification (MLPA) and next generation sequencing (NGS) were used for DNA analysis; HbA was measured with cation exchange HPLC (Bio-Rad Variant™ II; Arkray Adams HA-8180V; Tosoh HLC-723 G7), CE (Sebia Capillarys 2 Flex Piercing), boronate affinity HPLC (Trinity Biotech Hb9210 Premier), immunoassay (Cobas c501 Tina-quant HbA Gen. Read More

View Article and Full-Text PDF

Management of patients with X-linked hypophosphatemic rickets during Covid-19 pandemic lockdown.

J Pediatr Endocrinol Metab 2021 Apr 23. Epub 2021 Apr 23.

Division of Pediatrics, Department of Obstetrics, Gynecology and Pediatrics, University-Hospital, Pisa, Italy.

Objectives: To identify a safe pathway for management and treatment of patients with X-linked hypophosphatemic rickets (XLH) during Covid-19 pandemic lockdown.

Methods: Twenty-six patients with XLH (age 3.1-25. Read More

View Article and Full-Text PDF

Recent Progress in Flexible Tactile Sensors for Human-Interactive Systems: From Sensors to Advanced Applications.

Adv Mater 2021 Apr 22:e2005902. Epub 2021 Apr 22.

School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

Flexible tactile sensors capable of measuring mechanical stimuli via physical contact have attracted significant attention in the field of human-interactive systems. The utilization of tactile information can complement vision and/or sound interaction and provide new functionalities. Recent advancements in micro/nanotechnology, material science, and information technology have resulted in the development of high-performance tactile sensors that reach and even surpass the tactile sensing ability of human skin. Read More

View Article and Full-Text PDF

Combining payment for crop damages and reward for productivity to address wildlife conflict.

Conserv Biol 2021 Apr 22. Epub 2021 Apr 22.

E-1-8, Girija Shankar Vihar, Karve nagar, Pune, Maharashtra, India.

Conflict caused by wild herbivores damaging crops is an almost universal problem in conservation. We designed and implemented a game-theory-based system for supporting farmers whose crops were being heavily damaged by wild herbivores. In this community-operated system, farmers self-report their production, which is endorsed by neighboring farmers. Read More

View Article and Full-Text PDF

Pure Red Cell Aplasia following ABO-Mismatched Allogeneic Hematopoietic Stem Cell Transplantation: Resolution with Daratumumab Treatment.

Acta Haematol 2021 Apr 22:1-5. Epub 2021 Apr 22.

Department of Hematology and Bone Marrow Transplantation, Rambam Health Care Campus, Haifa, Israel.

Pure red cell aplasia (PRCA) can potentially occur after allogeneic hematopoietic stem cell transplantation (allo-HSCT) if recipient and donor ABO blood groups are mismatched, with the recipient having isoagglutinins against the donor blood group. Patient plasma cells that survive transplant conditioning produce anti-ABO isoagglutinins targeting donor erythroid precursors in the bone marrow and thus causing red cell aplasia. Therapeutic options include steroids, discontinuation of immunosuppression, plasmapheresis, donor lymphocyte infusion, rituximab, and bortezomib, all with limited benefit. Read More

View Article and Full-Text PDF

High-throughput monitoring of biomass conversion reaction with automatic time-resolved analysis.

J Chromatogr A 2021 Apr 9;1646:462145. Epub 2021 Apr 9.

Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, China. Electronic address:

Reactions of biomass conversions are of great importance in fine chemistry for substantial development. While numerous studies have been performed to search for functional materials to catalyze biomass conversions, a robust and high-throughput analytical method is rather limited, which may hamper further integration and automation of the reactions. Here we propose an automatic and sequential method for the investigation of glucose conversion. Read More

View Article and Full-Text PDF

Probing the role of Val228 on the catalytic activity of Scytalidium catalase.

Biochim Biophys Acta Proteins Proteom 2021 Apr 19:140662. Epub 2021 Apr 19.

Department of Biology, Kocaeli University, Umuttepe, Kocaeli 41380, Turkey. Electronic address:

Scytalidium catalase is a homotetramer including heme d in each subunit. Its primary function is the dismutation of HO to water and oxygen, but it is also able to oxidase various small organic compounds including catechol and phenol. The crystal structure of Scytalidium catalase reveals the presence of three linked channels providing access to the exterior like other catalases reported so far. Read More

View Article and Full-Text PDF

Endoscopic Proximal Hamstring Repair is Safe and Efficacious with High Patient Satisfaction at a Minimum of Two-Year Follow-up.

Arthroscopy 2021 Apr 19. Epub 2021 Apr 19.

Duke University Medical Center, Department of Orthopaedic Surgery.

Purpose: The purpose of this study was to evaluate the short-term outcomes of endoscopic proximal hamstring repair including clinical outcomes, patient reported outcomes, and complications.

Methods: A retrospective analysis was performed on consecutive patients who underwent endoscopic proximal hamstring repair from 2013-2018 by a senior sports medicine orthopaedic surgeon. Demographic, operative, clinical data, complications, and patient reported outcomes were collected and analyzed including the International Hip Outcome Tool-12 (iHOT-12), Single Assessment Numeric Evaluation (SANE), Modified Harris Hip Score (mHHS), and Hip Outcome Score (HOS). Read More

View Article and Full-Text PDF

Biophotonics for diagnostic detection of extracellular vesicles.

Adv Drug Deliv Rev 2021 Apr 19. Epub 2021 Apr 19.

IRCCS Fondazione Don Carlo Gnocchi ONLUS, Via Capecelatro 66, Milan 20148, Italy. Electronic address:

Extracellular Vesicles (EVs) are versatile carriers for biomarkers involved in the pathogenesis of multiple human disorders. Despite the increasing scientific and commercial interest in EV application in diagnostics, traditional biomolecular techniques usually require consistent sample amount, rely on operator-dependent and time- consuming procedures and cannot cope with the nano-size range of EVs, limiting both sensitivity and reproducibility of results. The application of biophotonics, i. Read More

View Article and Full-Text PDF

Colonic mucosal eosinophilia in children without inflammatory bowel disease.

Hum Pathol 2021 Apr 19. Epub 2021 Apr 19.

Children's Mercy, Division of Gastroenterology, Kansas City, MO, 64108.

Children undergoing colonoscopy and mucosal biopsies may show increased colonic mucosal eosinophils, which may or may not be associated with inflammatory bowel disease. There is not much clinical data on American children who have isolated increased colonic mucosal eosinophils. We sought to study the clinical correlates of children without inflammatory bowel disease who show increased mucosal eosinophils to understand their clinical presentation, etiological associations and outcome. Read More

View Article and Full-Text PDF

A continuous flow cell culture system for precision cell stimulation and time-resolved profiling of cell secretion.

Anal Biochem 2021 Apr 19:114213. Epub 2021 Apr 19.

Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, 08854, USA; Department of Medicine, Rutgers Biomedical Health Sciences, New Brunswick, New Jersey, 08852, USA. Electronic address:

Cells exchange substances with their surroundings during metabolism, signaling, and other functions. These fluxes are dynamic, changing in response to external cues and internal programs. Static cultures are inadequate for measuring these dynamics because the environments of the cells change as substances accumulate or deplete from medium, unintentionally affecting cell behavior. Read More

View Article and Full-Text PDF

The race to resolution of benign biliary strictures: Slow and steady vs pedal to the covered metal?

Gastroenterology 2021 Apr 19. Epub 2021 Apr 19.

Department of Gastroenterology, Hepatology, and Nutrition, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania. Electronic address:

View Article and Full-Text PDF

Illuminating new structures of a rod CNG channel: The eye's chemoelectrical converter.

Neuron 2021 Apr;109(8):1245-1247

Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA. Electronic address:

In this issue of Neuron, Xue et al. report high-resolution structures of the human cGMP-activated ion channel CNGA1 from rod photoreceptors. These structures provide valuable insights into the processes of cGMP-dependent activation and Ca block and permeation. Read More

View Article and Full-Text PDF

Visualization of Protein Coding, Long Noncoding, and Nuclear RNAs by Fluorescence in Situ Hybridization in Sections of Shoot Apical Meristems and Developing Flowers1[OPEN].

Plant Physiol 2020 Jan;182(1):147-158

Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom.

In addition to transcriptional regulation, gene expression is further modulated through mRNA spatiotemporal distribution, by RNA movement between cells, and by RNA localization within cells. Here, we have adapted RNA fluorescence in situ hybridization (FISH) to explore RNA localization in Arabidopsis (Arabidopsis thaliana). We show that RNA FISH on sectioned material can be applied to investigate the tissue and subcellular localization of meristem and flower development genes, cell cycle transcripts, and plant long noncoding RNAs. Read More

View Article and Full-Text PDF
January 2020

[General hospitals: inclusion in the healthcare networks, and factors that determine their performance].

Cien Saude Colet 2021 Apr 7;26(4):1275-1288. Epub 2019 Jun 7.

Programa de Pós-Graduação em Saúde Coletiva, Universidade Estadual de Londrina. Rodovia Celso Garcia Cid PR-445/Km 380, Campus Universitário. 86057-970 Londrina PR Brasil.

The scope of this study was to analyze the inclusion of general hospitals in Healthcare Networks and the factors that determine their participation in these networks. A "scoping review" was conducted with the selection of 14 articles published between January 2011 and June 2018. From the mapping of the results the categories of analysis were constituted: Hospital, regionalization and economy of scale; Fragility of network organization and hospital as first choice; and Hospital and evidence of (dis) integration of the Healthcare Networks. Read More

View Article and Full-Text PDF

Locked Anterior-Inferior Sternoclavicular Joint Dislocation: A Case Report.

JBJS Case Connect 2021 Apr 22;11(2). Epub 2021 Apr 22.

Department of Orthopaedic Surgery, Division of Shoulder and Elbow, New York University Langone Health, Grossman School of Medicine, New York, New York.

Case: We report a rare variant of sternoclavicular joint (SCJ) dislocation, namely locked anterior-inferior dislocation, with unique clinical, radiographic, and intraoperative findings. In this variant, the medial clavicle was displaced anteriorly and inferiorly and locked in the manubrial-intercostal space, with corresponding mechanical dysfunction of the ipsilateral shoulder girdle joints. Symptoms unique to this variant included painful neck spasms and limited glenohumeral elevation. Read More

View Article and Full-Text PDF

Noise Robust Face Hallucination Based on Smooth Correntropy Representation.

IEEE Trans Neural Netw Learn Syst 2021 Apr 22;PP. Epub 2021 Apr 22.

Face hallucination technologies have been widely developed during the past decades, among which the sparse manifold learning (SML)-based approaches have become the popular ones and achieved promising performance. However, these SML methods always failed in handling noisy images due to the least-square regression (LSR) they used for error approximation. To this end, we propose, in this article, a smooth correntropy representation (SCR) model for noisy face hallucination. Read More

View Article and Full-Text PDF

Exploiting wavelength diversity for high resolution time-of-flight 3D imaging.

IEEE Trans Pattern Anal Mach Intell 2021 Apr 22;PP. Epub 2021 Apr 22.

The poor lateral and depth resolution of state-of-the-art 3D sensors based on the time-of-flight (ToF) principle has limited widespread adoption to a few niche applications. In this work, we introduce a novel sensor concept that provides ToF-based 3D measurements of real world objects and surfaces with depth precision up to 35m and point cloud densities commensurate with the native sensor resolution of standard CMOS/CCD detectors (up to several megapixels). Such capabilities are realized by combining the best attributes of continuous wave ToF sensing, multi-wavelength interferometry, and heterodyne interferometry into a single approach. Read More

View Article and Full-Text PDF

SF: Stabilizing Transient Ions in Helium Nanodroplets.

J Phys Chem Lett 2021 Apr 22:4112-4117. Epub 2021 Apr 22.

Institut für Ionenphysik und Angewandte Physik, Universität Innsbruck, A-6020 Innsbruck, Austria.

There are myriad ions that are deemed too short-lived to be experimentally accessible. One of them is SF. It has never been observed, although not for lack of trying. Read More

View Article and Full-Text PDF

Nanoscale Bilayer Mechanical Lithography Using Water as Developer.

Nano Lett 2021 Apr 22. Epub 2021 Apr 22.

Department of Materials, University of Oxford, Parks Road, Oxford OX1 3PH, United Kingdom.

Sustainability has become a critical concern in the semiconductor industry as hazardous wastes released during the manufacturing process of semiconductor devices have an adverse impact on human beings and the environment. The use of hazardous solvents in existing fabrication processes also restricts the use of polymer substrates because of their low chemical resistance to such solvents. Here, we demonstrate an environmentally friendly mechanical, bilayer lithography that uses just water for development and lift-off. Read More

View Article and Full-Text PDF

Rapid Detection of Viral Envelope Lipids Using Lithium Adducts and AP-MALDI High-Resolution Mass Spectrometry.

J Am Soc Mass Spectrom 2021 Apr 22. Epub 2021 Apr 22.

Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, Maryland 21201, United States.

There is an unmet need to develop analytical strategies that not only characterize the lipid composition of the viral envelope but also do so on a time scale that would allow for high-throughput analysis. With that in mind, we report the use of atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) high-resolution mass spectrometry (HRMS) combined with lithium adduct consolidation to profile total lipid extracts rapidly and confidently from enveloped viruses. The use of AP-MALDI reduced the dependency of using a dedicated MALDI mass spectrometer and allowed for interfacing the MALDI source to a mass spectrometer with the desired features, which included high mass resolving power (>100000) and tandem mass spectrometry. Read More

View Article and Full-Text PDF

Novel 2,5-Diarylcyclopentenone Derivatives from the Wild Edible Mushroom and Their Antioxidant Activities.

J Agric Food Chem 2021 Apr 22. Epub 2021 Apr 22.

Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, People's Republic of China.

Wild edible mushrooms are important as a source of nutraceuticals and for the discovery of bioactive metabolites as pharmaceuticals. In this work, 10 rare 2,5-diarylcyclopentenone derivatives were isolated from the wild edible mushroom (Batsch) Fr., including eight novel compounds termed involutenone A-H () and two previously identified compounds (). Read More

View Article and Full-Text PDF

Hydrophilic AIE-Active Tetraarylethenes for Fluorescence Sensing and Super-Resolution Imaging of Amyloid Fibrils from Hen Egg White Lysozyme.

ACS Appl Mater Interfaces 2021 Apr 22. Epub 2021 Apr 22.

Wuhan National Laboratory for Optoelectronics, School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

Hen egg white lysozyme (HEWL) is frequently applied as a model protein for research on protein folding, unfolding, and fibrillization identified by featured fluorescent probes. Here, a series of hydrophilic, pH-sensitive tetraarylethene (TAE)-type AIEgens are synthesized via a geminal cross-coupling (GCC) reaction and evaluated for their capabilities of fluorescence sensing and super-resolution localization imaging of HEWL fibrils. With superior optical and sensing properties, the selected TAE-type AIEgen probe is weakly emissive in aqueous media, without dependence on the pH value and buffer concentration, but exhibits "turn-on" fluorescence upon interaction with HEWL amyloid fibrils in a spontaneous and reversible way that just meets the requirement of fluorescence random switching for super-resolution imaging. Read More

View Article and Full-Text PDF

Organelle-Selective Labeling of Choline-Containing Phospholipids (CPLs) and Real-Time Imaging in Living Cells.

Curr Protoc 2021 Apr;1(4):e105

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Japan.

Choline-containing phospholipids (CPLs), including phosphatidylcholine (PC) and sphingomyelin (SM), are the major components of mammalian cell membranes and play critical roles during a variety of cellular processes. However, intracellular dynamics of CPLs is poorly understood due to a lack of methods to trace CPL trafficking at organelle resolution. Here, we describe protocols that make it possible to fluorescently label CPLs at the targeted organelles and to monitor their movement within living cells using confocal microscopy. Read More

View Article and Full-Text PDF

Enantioselective Preparation of Planar-Chiral Transition Metal Complexes by Asymmetric Olefin-Metathesis Reactions in Metal Coordination Spheres.

Chem Rec 2021 Apr 22. Epub 2021 Apr 22.

Department of Natural Science Graduate School of Science and Technology and Tokushima International Science Institute, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima, 770-8506, Japan.

Planar-chiral transition metal complexes are useful chiral auxiliaries in organic and organometallic chemistry, and they have been utilized as chiral ligands, chiral catalysts, or chiral building blocks, etc. Despite the importance of such planar-chiral species in asymmetric synthesis, their preparation in optically active forms is still a challenging problem. Indeed, reported examples of catalytic enantioselective synthesis of planar-chiral complexes have been rare, and this has been a developing area in this field. Read More

View Article and Full-Text PDF

Effects of nFeO capping on phosphorus release from sediments in a eutrophic lake.

Environ Sci Pollut Res Int 2021 Apr 22. Epub 2021 Apr 22.

College of Hydrology and Water Resources, Hohai University, Nanjing, 210098, China.

This study applied the techniques of high-resolution dialysis (HR-Peeper) and diffusive gradients in thin films (DGT) to explore the effects and the behind mechanism for inhibition phosphorus (P) releasing from sediments by nFeO capping. The highest decreasing rates of SRP and labile P (i.e. Read More

View Article and Full-Text PDF

Tissue and/or developmental stage specific methylation of nrDNA in Capsicum annuum.

J Plant Res 2021 Apr 22. Epub 2021 Apr 22.

Department of Field Crops, Faculty of Agriculture, Akdeniz University, 07059, Antalya, Turkey.

The nuclear ribosomal DNA (nrDNA) sequences are often used for phylogenetic analysis among organisms. Because DNA cytosine methylation and nucleolar dominancy are two common epigenetic mechanisms of nrDNA, we hypothesized that internal transcribed spacer 1 (ITS1), 5.8S rRNA and ITS2 of nrDNA sequences could be used as epigenetic biomarkers. Read More

View Article and Full-Text PDF

Gene Networks Underlying Cannabinoid and Terpenoid Accumulation in Cannabis.

Plant Physiol 2019 Jul;180(4):1877-1897

Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, Washington 99164-6340.

Glandular trichomes are specialized anatomical structures that accumulate secretions with important biological roles in plant-environment interactions. These secretions also have commercial uses in the flavor, fragrance, and pharmaceutical industries. The capitate-stalked glandular trichomes of Cannabis sativa (cannabis), situated on the surfaces of the bracts of the female flowers, are the primary site for the biosynthesis and storage of resins rich in cannabinoids and terpenoids. Read More

View Article and Full-Text PDF

Regulation of Parent-of-Origin Allelic Expression in the Endosperm.

Plant Physiol 2019 Jun;180(3):1498-1519

Section for Genetics and Evolutionary Biology, Department of Biosciences, University of Oslo, 0316 Oslo, Norway.

Genomic imprinting is an epigenetic phenomenon established in the gametes prior to fertilization that causes differential expression of parental alleles, mainly in the endosperm of flowering plants. The overlap between previously identified panels of imprinted genes is limited. To investigate imprinting, we used high-resolution sequencing data acquired with sequence-capture technology. Read More

View Article and Full-Text PDF

Turnip Mosaic Virus Components Are Released into the Extracellular Space by Vesicles in Infected Leaves.

Plant Physiol 2019 Jun;180(3):1375-1388

Department of Biology, McGill University, Montréal, Québec, H3A 1B1, Canada.

Turnip mosaic virus (TuMV) reorganizes the endomembrane system of the infected cell to generate endoplasmic-reticulum-derived motile vesicles containing viral replication complexes. The membrane-associated viral protein 6K2 plays a key role in the formation of these vesicles. Using confocal microscopy, we observed that this viral protein, a marker for viral replication complexes, localized in the extracellular space of infected Nicotiana benthamiana leaves. Read More

View Article and Full-Text PDF