132,277 results match your criteria resistance genes

Draft genome sequence of a carbapenamase-producing (bla) and multidrug-resistant hypervirulent Klebsiella pneumoniae isolate (ST11) from Pakistan, with a non-hypermucoviscous phenotype associated with rmpA2 mutation.

J Glob Antimicrob Resist 2021 May 12. Epub 2021 May 12.

University of Reading, Quaid-i-Azam University. Electronic address:

Introduction: ST11 is a high-risk sequence type associated with carbapenem-resistant K. pneumoniae strains. Carbapenemase-producing (CP) hypervirulent K. Read More

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Creation and judicious application of a wheat resistance gene atlas.

Mol Plant 2021 May 12. Epub 2021 May 12.

John Innes Centre, Norwich Research Park, Norwich, UK.

Disease resistance (R) gene cloning in wheat (Triticum aestivum) has been accelerated by the recent surge of genomic resources, facilitated by advances in sequencing technologies and bioinformatics. However, with the challenges of population growth and climate change, it is vital not only to clone and functionally characterise a few handfuls of R genes, but to do so at a scale that would facilitate the breeding and deployment of crops that can recognise the wide range of pathogen effectors that threaten agroecosystems. Pathogen populations are continually changing, and breeders must have tools and resources available to rapidly respond to those changes if we are to safeguard our daily bread. Read More

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A comparative study for phosphate adsorption on amorphous FeOOH and goethite (α-FeOOH): an investigation of relationship between the surface chemistry and structure.

Environ Res 2021 May 12:111223. Epub 2021 May 12.

Department of Civil Engineering, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA. Electronic address:

Eutrophication is generally caused by excess nitrogen and phosphorus being released into surface waters by runoff. Developing adsorbents for adsorbing phosphate within soil buffer zones and/or water treatment columns may be effective methods to mitigate this problem. In this study, an amorphous FeOOH (AF) and a well-crystallized α-FeOOH (CF) was formulated to compare phosphate adsorption behavior. Read More

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Identification of novel drug resistance mechanisms by genomic and transcriptomic profiling of glioblastoma cells with mutation-activated EGFR.

Life Sci 2021 May 12:119601. Epub 2021 May 12.

Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Mainz, Germany. Electronic address:

Aims: Epidermal growth factor receptor (EGFR) is not only involved in carcinogenesis, but also in chemoresistance. We characterized U87.MGΔEGFR glioblastoma cells with constitutively active EGFR due to deletion at the ligand binding domain in terms of gene expression profiling and chromosomal aberrations. Read More

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Antisense oligonucleotide-mediated knockdown of Mpzl3 attenuates the negative metabolic effects of diet-induced obesity in mice.

Physiol Rep 2021 May;9(9):e14853

Department of Anesthesiology & Perioperative Medicine, Penn State University College of Medicine, Hershey, PA, USA.

Previously, we demonstrated that global knockout (KO) of the gene encoding myelin protein zero-like 3 (Mpzl3) results in reduced body weight and adiposity, increased energy expenditure, and reduced hepatic lipid synthesis in mice. These mice also exhibit cyclic and progressive alopecia which may contribute to the observed hypermetabolic phenotype. The goal of the current study was to determine if acute and peripherally restricted knockdown of Mpzl3 could ameliorate the negative metabolic effects of exposure to a high-fat and sucrose, energy-dense (HED) diet similar to what was observed in global Mpzl3 KO mice in the absence of a skin phenotype. Read More

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Elevated fibroblast growth factor-inducible 14 expression transforms proneural-like gliomas into more aggressive and lethal brain cancer.

Glia 2021 May 15. Epub 2021 May 15.

Department of Neurosurgery, University of Maryland School of Medicine, Baltimore, Maryland, USA.

High-grade gliomas (HGGs) are aggressive, treatment-resistant, and often fatal human brain cancers. The TNF-like weak inducer of apoptosis (TWEAK)/fibroblast growth factor-inducible 14 (Fn14) signaling axis is involved in tissue repair after injury and constitutive signaling has been implicated in the pathogenesis of numerous solid cancers. The Fn14 gene is expressed at low levels in the normal, uninjured brain but is highly expressed in primary isocitrate dehydrogenase wild-type and recurrent HGGs. Read More

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Retrospective whole-genome comparison of Salmonella enterica serovar Enteritidis from foodborne outbreaks in Southern Brazil.

Braz J Microbiol 2021 May 14. Epub 2021 May 14.

Lutheran University of Brazil, 8001, Canoas, Rio Grande Do Sul, 92425-020, Brazil.

Salmonella enterica serovar Enteritidis is frequently isolated from animal-source foods associated with human salmonellosis outbreaks. This serovar was spread to animal (mainly poultry) farms worldwide in the 1980s, and it is still detected in foods produced in many countries, including Brazil. The present study reports a retrospective genome-wide comparison of S. Read More

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Molecular identification of triticale introgression lines carrying leaf rust resistance genes transferred from Aegilops kotschyi Boiss. and Ae. tauschii Coss.

J Appl Genet 2021 May 14. Epub 2021 May 14.

Department of Genomics, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland.

Triticale (× Triticosecale Wittmack) is a commercial hybrid harboring wheat (Triticum sp.) and rye (Secale cereale L.) genomes. Read More

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In vivo Engineering of Chromosome 19 q-arm by Employing the CRISPR/AsCpf1 and ddAsCpf1 Systems in Human Malignant Gliomas (Hypothesis).

J Mol Neurosci 2021 May 15. Epub 2021 May 15.

Department of Medical Genetics. Shahid, Beheshti University of Medical Sciences, Tehran, Iran.

Deletions of the q13.3 region of chromosome 19 have been found commonly in all three main kinds of diffuse human malignant gliomas, powerfully demonstrating the existence of tumor suppressor genes in this region. Consistent with the previous studies, the most common deletion interval has been mapped to a roughly 4 Mb region of 19q13. Read More

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Blueberry-derived exosomes-like nanoparticles ameliorate nonalcoholic fatty liver disease by attenuating mitochondrial oxidative stress.

Acta Pharmacol Sin 2021 May 14. Epub 2021 May 14.

Chongqing Key Lab of Medicinal Chemistry & Molecular Pharmacology, Chongqing University of Technology, Chongqing, 400054, China.

Accumulating evidence indicates that mitochondrial dysfunction and oxidative stress play a pivotal role in the initiation and progression of nonalcoholic fatty liver disease (NAFLD). In this study, we found that blueberry-derived exosomes-like nanoparticles (BELNs) could ameliorate oxidative stress in rotenone-induced HepG2 cells and high-fat diet (HFD)-fed C57BL/6 mice. Preincubation with BELNs decreased the level of reactive oxygen species (ROS), increased the mitochondrial membrane potential, and prevented cell apoptosis by inducing the expression of Bcl-2 and heme oxygenase-1 (HO-1) and decreasing the content of Bax in rotenone-treated HepG2 cells. Read More

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Beta cell adaptation to pregnancy requires prolactin action on both beta and non-beta cells.

Sci Rep 2021 May 14;11(1):10372. Epub 2021 May 14.

Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, T2N 1N4, Canada.

Pancreatic islets adapt to insulin resistance of pregnancy by up regulating β-cell mass and increasing insulin secretion. Previously, using a transgenic mouse with global, heterozygous deletion of prolactin receptor (Prlr), we found Prlr signaling is important for this adaptation. However, since Prlr is expressed in tissues outside of islets as well as within islets and prolactin signaling affects β-cell development, to understand β-cell-specific effect of prolactin signaling in pregnancy, we generated a transgenic mouse with an inducible conditional deletion of Prlr from β-cells. Read More

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Antimicrobial-resistance gene transfer of in mono- and dual-species biofilms.

Appl Environ Microbiol 2021 May 14. Epub 2021 May 14.

Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

Horizontal gene transfer (HGT) is a driving force for the dissemination of antimicrobial resistance (AMR) genes among , a leading cause of foodborne gastroenteritis worldwide Although HGT is well documented for planktonic cells, the role of biofilms in AMR spread that likely to occur in the environment is poorly understood. Here, we developed a co-cultivation model to investigate the HGT of chromosomally-encoded AMR genes between two F38011 AMR mutants in biofilms. Compared to planktonic cells, biofilms significantly promoted HGT ( < 0. Read More

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WSM419 genes that improve symbiosis between Rm1021 and Jemalong A17 and in other symbiotic systems.

Appl Environ Microbiol 2021 May 14. Epub 2021 May 14.

Institute of Biological Chemistry and School of Molecular Biosciences, Washington State University, Pullman, WA 99164 USA

Some soil bacteria called rhizobia can interact symbiotically with legumes in which they form nodules on the plant roots where they can reduce atmospheric dinitrogen to ammonia, a form of nitrogen that can be used by growing plants. Rhizobia/plant combinations can differ in how successful this symbiosis is- Rm1021 forms a relatively ineffective symbiosis with Jemalong A17 but WSM419 is able to support more vigorous plant growth. Using proteomic data from free-living and symbiotic WSM419, we previously identified a subset of proteins that were not closely related to any Rm1021 proteins and speculated that adding one or more of these proteins to Rm1021 would increase its effectiveness on A17. Read More

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Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems.

Microbiome 2021 May 14;9(1):108. Epub 2021 May 14.

Interdisciplinary Research Center for Soil Microbial Ecology and Land Sustainable Productivity in Dry Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Background: Soil is an important reservoir of antibiotic resistance genes (ARGs), but their potential risk in different ecosystems as well as response to anthropogenic land use change is unknown. We used a metagenomic approach and datasets with well-characterized metadata to investigate ARG types and amounts in soil DNA of three native ecosystems: Alaskan tundra, US Midwestern prairie, and Amazon rainforest, as well as the effect of conversion of the latter two to agriculture and pasture, respectively.

Results: High diversity (242 ARG subtypes) and abundance (0. Read More

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Use of Circulating Tumor DNA for the Clinical Management of Metastatic Castration-Resistant Prostate Cancer: A Multicenter, Real-World Study.

J Natl Compr Canc Netw 2021 May 14:1-10. Epub 2021 May 14.

1Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai.

Background: This study aimed to describe the aberrations of DNA damage repair genes and other important driving genes in Chinese patients with metastatic castration-resistant prostate cancer (mCRPC) using circulating tumor (ctDNA) sequencing and to evaluate the associations between the clinical outcomes of multiple therapies and key genomic alterations in mCRPC, especially DNA damage repair genes.

Patients And Methods: A total of 292 Chinese patients with mCRPC enrolled from 8 centers. Multigene targeted sequencing was performed on 306 ctDNA samples and 23 matched tumor biopsies. Read More

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Diversity, abundance and expression of the antibiotic resistance genes in a Chinese landfill: Effect of deposit age.

J Hazard Mater 2021 May 5;417:126027. Epub 2021 May 5.

Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address:

Landfills are the hotspots for the occurrence of antibiotic resistance genes (ARGs) in the environment. However, limited information is available on the profile of ARGs in response to the varying age of refuse in landfills. In this study, the diversity, abundance and expression of ARGs in a Chinese landfill were assessed by high-throughput quantitative PCR. Read More

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Thymoquinone sensitizes human hepatocarcinoma cells to TRAIL-induced apoptosis via oxidative DNA damage.

DNA Repair (Amst) 2021 Apr 15;103:103117. Epub 2021 Apr 15.

Department of General Surgery, the First Hospital Affiliated of Shandong First Medical University (Qianfoshan Hospital), Jinan, Shandong, 250014, China. Electronic address:

Introduction: Hepatocellular carcinoma (HCC) remains one of the most predominant types of digestive system malignancies worldwide. TNF-related apoptosis-inducing ligand (TRAIL) is a biological cytokine with the mentioned specificity, but some tumor cells' resistance limits its use as a therapeutic approach. The present study aimed to investigate thymoquinone (TQ) and TRAIL's combined effect and the potential mechanisms in human hepatic HepG2 carcinoma cells. Read More

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Research Note: Characterization of antibiotic resistant phenotypes and linked genes of Escherichia coli and Klebsiella pneumoniae from healthy broiler chickens, Karnataka, India.

Poult Sci 2021 Mar 22;100(6):101094. Epub 2021 Mar 22.

Animal Disease Diagnostic Laboratory, Government Veterinary Hospital, Jail Road, Karangalpady, Mangalore, 575002, Karnataka, India.

The study was carried out to estimate the burden and pattern of antibiotic resistance and to identify antibiotic resistance genes with focus on ESBL producers, plasmid mediated quinolone resistance, and tetracycline efflux genes, in faecal bacterial isolates collected from poultry farms of coastal Southern Karnataka, India. High resistance to fluoroquinolones was observed with 94% Escherichia coli and 80% Klebsiella pneumoniae being resistant to both ciprofloxacin and levofloxacin. All the Escherichia coli strains were resistant to tetracycline (100%). Read More

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Co-occurrence of crAssphage and antibiotic resistance genes in agricultural soils of the Yangtze River Delta, China.

Environ Int 2021 May 11;156:106620. Epub 2021 May 11.

College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Hangzhou 310058, China. Electronic address:

Agricultural soil is highly susceptible to manure contamination and thus is a potential source for the spread of pathogens and antibiotic resistance genes (ARGs). Routine monitoring fecal contamination in agricultural soil can reduce the manure-derived ARG contaminations. This study investigated the distribution of crAssphage, a highly human-specific indicator of fecal pollution, in agricultural soils in the Yangtze River Delta (YRD) of China, and its potential in serving as an indicator of soil ARGs. Read More

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Resistance elicited by sub-lethal concentrations of ampicillin is partially mediated by quorum sensing in Pseudomonas aeruginosa.

Environ Int 2021 May 11;156:106619. Epub 2021 May 11.

School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China. Electronic address:

The rapid increase of antibiotic resistance is a serious challenge around the world. Antibiotics are present in various environments at sub-lethal concentrations, but how resistance emerges under sub-lethal conditions is not fully clear. In this study, we evolved Pseudomonas aeruginosa PAO1 under sub-lethal conditions, in the presence of either 15-30 μg/mL or 150-300 μg/mL of ampicillin. Read More

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Multiomics reveals pivotal roles of Na + translocation and compartmentation in regulating salinity resistance in allotetraploid rapeseed.

J Exp Bot 2021 May 14. Epub 2021 May 14.

School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.

Vastness and complexity of the allotetraploid rapeseed genome is a huge challenge for understanding its salinity resistance. Herein, rapeseed plants of a salinity-resistant genotype (H159) and a salinity-sensitive genotype (L339) exhibited significantly differential salinity resistance. Integrated analysis of phenome, ionome, genome, and transcriptome was performed to identify the mechanisms underlying differential salinity resistance. Read More

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Extramedullary multiple myeloma patient derived orthotopic xenograft with high disturbed genome: combined exhaustive molecular and therapeutic studies.

Dis Model Mech 2021 May 14. Epub 2021 May 14.

Department of Clinical Hematology, Catalan Institute of Oncology, Bellvitge Biomedical Research Institute, Universitat de Barcelona, L'Hospitalet del Llobregat Barcelona, Spain.

Extramedullary multiple myeloma (EMM) has an overall survival of 6 months and occurs in 20% of multiple myeloma (MM) patients. Genetic and epigenetic mechanisms involved in EMM and the therapeutic role of new agents for MM are not well established. Besides, well characterized preclinical models for EMM are not available. Read More

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Transcription Factor CsWRKY65 Participates in the Establishment of Disease Resistance of Citrus Fruits to .

J Agric Food Chem 2021 May 14. Epub 2021 May 14.

College of Food Science, Southwest University, Chongqing 400715, P. R. China.

is the primary pathogen that causes serious yield losses worldwide. In our previous study, CsWRKY transcription factors (TFs) and some genes associated with immunity were identified in citrus fruits after infection, but little information is available in the literature on the mechanisms of TFs in citrus disease resistance. In this study, the possible mechanisms of CsWRKY65 participating in the establishment of disease resistance were investigated. Read More

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Fine mapping of a powdery mildew resistance gene MlIW39 derived from wild emmer wheat (Triticum turgidum ssp. dicoccoides).

Theor Appl Genet 2021 May 13. Epub 2021 May 13.

State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.

Key Message: Powdery mildew resistance gene MlIW39, originated from wild emmer wheat accession IW39, was mapped to a 460.3 kb genomic interval on wheat chromosome arm 2BS. Wheat powdery mildew, caused by Blumeria graminis f. Read More

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BET inhibitors repress expression of Interferon-stimulated genes and synergize with HDAC inhibitors in glioblastoma.

Neuro Oncol 2021 May 14. Epub 2021 May 14.

Service of Neurosurgery, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.

Background: The development of rational combination therapies is key to overcome inherent treatment resistance of glioblastoma. We aim at identifying new druggable targets by disturbing glioblastoma cells with inhibitors of Bromodomain and Extra-Terminal motif (BET) proteins to reveal cancer relevant vulnerabilities that may sensitize to a second drug. BET-proteins are epigenetic modulators and have been associated with proto-oncogene overexpression in cancer. Read More

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Genetic architecture of the metabolic pathway of salicylic acid biosynthesis in Populus.

Tree Physiol 2021 May 13. Epub 2021 May 13.

National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.

Salicylic acid (SA) is a vital hormone for adaptive responses to biotic and abiotic stresses, which facilitating growth-immunity trade-offs in plants. However, the genetic regulatory networks underlying the metabolic pathway of SA biosynthesis in perennial species remain unclear. Here, we integrated genome-wide association study (GWAS) with metabolite and expression profiling methodologies to dissect the genetic architecture of SA biosynthesis in Populus. Read More

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Complete genome sequence of strain K_SA184, multidrug resistance bacterium isolated from lamb ().

J Anim Sci Technol 2021 Jan 31;63(1):194-197. Epub 2021 Jan 31.

Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Korea.

is a representative foodborne pathogen in the world. The strain K_SA184 was isolated from the lamb (), which was collected from a local traditional market in South Korea. In this study, the strain K_SA184 was sequenced using PacBio RS II and Illumina NextSeq 500 platforms. Read More

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January 2021

High Prevalence of in Smoked Duck: Antibiotic and Heat Resistance, Virulence, and Genetics of the Isolates.

Food Sci Anim Resour 2021 Mar 1;41(2):324-334. Epub 2021 Mar 1.

Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea.

This study aimed at determining the genetic and virulence characteristics of the from smoked ducks. was isolated by plating, and the isolated colonies were identified by PCR. All the obtained seven isolates possessed the virulence genes (A, B, B, and A) and a 385 bp A amplicon. Read More

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Clinical and molecular characteristics of isolates at a teaching hospital in Shanghai, China.

Ann Transl Med 2021 Apr;9(8):668

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.

Background: (. ) has recently emerged as a cause of life-threatening nosocomial infections in humans. This study aims to investigate the clinical characteristics, homology, and antimicrobial patterns of . Read More

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Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells.

Ann Transl Med 2021 Apr;9(8):628

Anhui Province Key Laboratory of Medical Physics and Technology/Institute of Health and Medical Technology, Hefei Institutes of Physical Sciences, Chinese Academy of Sciences, Hefei, China.

Background: Acquired radioresistant cells exhibit many characteristic changes which may influence cancer progression and further treatment options. The purpose of this study is to investigate the changes of radioresistant human epidermal growth factor receptor 2 (HER2)-positive breast cancer cells on both phenotypic and molecular levels.

Methods: We established an acquired radioresistant cell line from its parental NF639 cell line (HER2-positive) by fractionated radiation and assessed changes in cellular morphology, proliferation, migration, anti-apoptosis activity, basal reactive oxygen species (ROS) level and energy metabolism. Read More

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