58,773 results match your criteria resistance detected


CTRP3 promotes TNF-α-induced apoptosis and barrier dysfunction in salivary epithelial cells.

Cell Signal 2021 May 12:110042. Epub 2021 May 12.

Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, China. Electronic address:

Background: C1q/tumour necrosis factor-related protein 3 (CTRP3) plays important roles in metabolism and inflammatory responses in various cells and tissues. However, the expression and function of CTRP3 in salivary glands have not been explored.

Methods: The expression and distribution of CTRP3 were detected by western blot, polymerase chain reaction, immunohistochemical and immunofluorescence staining. Read More

View Article and Full-Text PDF

High prevalence of BRAF in patients with cholestasis, sclerosing cholangitis or liver fibrosis secondary to Langerhans cell histiocytosis.

Pediatr Blood Cancer 2021 May 15:e29115. Epub 2021 May 15.

Hematology/Oncology Department, Hospital de Pediatria SAMIC Prof Dr Juan P Garrahan, Buenos Aires, Argentina.

Targeted therapies with MAPK inhibitors have proven to modulate the clinical manifestations of patients with Langerhans cell histiocytosis (LCH). We explored the presence of BRAF mutation in our cohort of patients with LCH and cholestasis, sclerosing cholangitis, or liver fibrosis that presented resistance to chemotherapy. The BRAF mutation was detected either in the diagnosis (skin and bone) or liver biopsy in our cohort of 13 patients. Read More

View Article and Full-Text PDF

Retrospective whole-genome comparison of Salmonella enterica serovar Enteritidis from foodborne outbreaks in Southern Brazil.

Braz J Microbiol 2021 May 14. Epub 2021 May 14.

Lutheran University of Brazil, 8001, Canoas, Rio Grande Do Sul, 92425-020, Brazil.

Salmonella enterica serovar Enteritidis is frequently isolated from animal-source foods associated with human salmonellosis outbreaks. This serovar was spread to animal (mainly poultry) farms worldwide in the 1980s, and it is still detected in foods produced in many countries, including Brazil. The present study reports a retrospective genome-wide comparison of S. Read More

View Article and Full-Text PDF

In vivo Engineering of Chromosome 19 q-arm by Employing the CRISPR/AsCpf1 and ddAsCpf1 Systems in Human Malignant Gliomas (Hypothesis).

J Mol Neurosci 2021 May 15. Epub 2021 May 15.

Department of Medical Genetics. Shahid, Beheshti University of Medical Sciences, Tehran, Iran.

Deletions of the q13.3 region of chromosome 19 have been found commonly in all three main kinds of diffuse human malignant gliomas, powerfully demonstrating the existence of tumor suppressor genes in this region. Consistent with the previous studies, the most common deletion interval has been mapped to a roughly 4 Mb region of 19q13. Read More

View Article and Full-Text PDF

Comparison of antimicrobial-resistant between urban raccoons and domestic dogs.

Appl Environ Microbiol 2021 May 14. Epub 2021 May 14.

Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, Minnesota, USA.

Wildlife can be exposed to antimicrobial resistant bacteria (ARB) via multiple pathways. Spatial overlap with domestic animals is a prominent exposure pathway. However, most studies of wildlife - domestic animal interfaces have focused on livestock and little is known about the wildlife - companion animal interface. Read More

View Article and Full-Text PDF

Diversity, abundance and expression of the antibiotic resistance genes in a Chinese landfill: Effect of deposit age.

J Hazard Mater 2021 May 5;417:126027. Epub 2021 May 5.

Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address:

Landfills are the hotspots for the occurrence of antibiotic resistance genes (ARGs) in the environment. However, limited information is available on the profile of ARGs in response to the varying age of refuse in landfills. In this study, the diversity, abundance and expression of ARGs in a Chinese landfill were assessed by high-throughput quantitative PCR. Read More

View Article and Full-Text PDF

Research Note: Characterization of antibiotic resistant phenotypes and linked genes of Escherichia coli and Klebsiella pneumoniae from healthy broiler chickens, Karnataka, India.

Poult Sci 2021 Mar 22;100(6):101094. Epub 2021 Mar 22.

Animal Disease Diagnostic Laboratory, Government Veterinary Hospital, Jail Road, Karangalpady, Mangalore, 575002, Karnataka, India.

The study was carried out to estimate the burden and pattern of antibiotic resistance and to identify antibiotic resistance genes with focus on ESBL producers, plasmid mediated quinolone resistance, and tetracycline efflux genes, in faecal bacterial isolates collected from poultry farms of coastal Southern Karnataka, India. High resistance to fluoroquinolones was observed with 94% Escherichia coli and 80% Klebsiella pneumoniae being resistant to both ciprofloxacin and levofloxacin. All the Escherichia coli strains were resistant to tetracycline (100%). Read More

View Article and Full-Text PDF

Tracking COVID-19 with wastewater to understand asymptomatic transmission.

Int J Infect Dis 2021 May 11. Epub 2021 May 11.

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Antimicrobial Resistance and Stewardship Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Electronic address:

Introduction: SARS-CoV-2 RNA is excreted in feces of most patients and therefore viral load in wastewater can be used as a surveillance tool to develop an early warning system to help and manage future pandemics.

Methods: We collected wastewater from 24 random locations at Bangkok city center and 26 nearby suburbs from July to December 2020. SARS-CoV-2 RNA copy numbers were measured using real-time polymerase chain reaction (PCR). Read More

View Article and Full-Text PDF

Extramedullary multiple myeloma patient derived orthotopic xenograft with high disturbed genome: combined exhaustive molecular and therapeutic studies.

Dis Model Mech 2021 May 14. Epub 2021 May 14.

Department of Clinical Hematology, Catalan Institute of Oncology, Bellvitge Biomedical Research Institute, Universitat de Barcelona, L'Hospitalet del Llobregat Barcelona, Spain.

Extramedullary multiple myeloma (EMM) has an overall survival of 6 months and occurs in 20% of multiple myeloma (MM) patients. Genetic and epigenetic mechanisms involved in EMM and the therapeutic role of new agents for MM are not well established. Besides, well characterized preclinical models for EMM are not available. Read More

View Article and Full-Text PDF

Evaluating the Presence of Replication-Competent SARS-CoV-2 from Nursing Home Residents with Persistently Positive RT-PCR Results.

Clin Infect Dis 2021 May 14. Epub 2021 May 14.

COVID-19 Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Replication-competent virus has not been detected in individuals with mild to moderate COVID-19 more than 10 days after symptom onset. It is unknown whether these findings apply to nursing home residents. Of 273 specimens collected from nursing home residents >10 days from the initial positive test, none were culture positive. Read More

View Article and Full-Text PDF

Multidrug-resistant tuberculosis imported into low-incidence countries-a GeoSentinel analysis, 2008-2020.

J Travel Med 2021 May 12. Epub 2021 May 12.

Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden.

Background: Early detection of imported multidrug-resistant tuberculosis (MDR-TB) is crucial, but knowledge gaps remain about migration- and travel-associated MDR-TB epidemiology. The aim was to describe epidemiologic characteristics among international travelers and migrants with MDR-TB.

Methods: Clinician-determined and microbiologically confirmed MDR-TB diagnoses deemed to be related to travel or migration were extracted from GeoSentinel, a global surveillance network of travel and tropical medicine clinics, from January 2008 through December 2020. Read More

View Article and Full-Text PDF

The Story of a Ship Journey, Malaria, and the Gene IVS-II-745 Mutation: Circassian Immigration to Cyprus.

Glob Med Genet 2021 Jun 16;8(2):69-71. Epub 2021 Mar 16.

Department of Medical Genetics, Faculty of Medicine, Erciyes University, Kayseri, Turkey.

 During 19th century, the Circassians were secluded from their lands and forced to migrate to Ottoman Empire properties. Approximately 2,346 Circassians were exiled from Istanbul to Cyprus Island. During the deportation journey, many of Circassian passed away in consequence of malaria and unknown reasons. Read More

View Article and Full-Text PDF

Forkhead box O1 targeting replication factor C subunit 2 expression promotes glioma temozolomide resistance and survival.

Ann Transl Med 2021 Apr;9(8):692

Department of Neurology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Background: Additional mechanisms of temozolomide (TMZ) resistance in gliomas remain uncertain. The aim of this study was to identify another DNA repair mechanism involving forkhead box O1 (FoxO1) and replicator C2 (RFC2) in gliomas.

Methods: We established glioma cells against TMZ, U87R, by exposure to TMZ. Read More

View Article and Full-Text PDF

Plasma mutation abundance affects clinical response to first-line EGFR-TKIs in patients with advanced non-small cell lung cancer.

Ann Transl Med 2021 Apr;9(8):635

Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: Activated epidermal growth factor receptor () mutation is the main pathogenic cause of non-small cell lung cancer (NSCLC) in Asia. However, the impact of plasma mutation abundance, especially of the ultra-low abundance of mutation detected by highly sensitive techniques on clinical outcomes of first-line EGFR tyrosine kinase inhibitors (TKIs) for advanced NSCLC patients remains unclear.

Methods: We qualitatively detected baseline status of NSCLC tissues using amplification-refractory mutation system and quantified the plasma abundance of mutations through next-generation sequencing (NGS). Read More

View Article and Full-Text PDF

Development and Preliminary Application of Multiplex Loop-Mediated Isothermal Amplification Coupled With Lateral Flow Biosensor for Detection of .

Front Cell Infect Microbiol 2021 27;11:666492. Epub 2021 Apr 27.

Key Laboratory of Major Diseases in Children, Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, China.

The aim of this study was to develop a simple and reliable method to detect (MTBC) and verify its clinical application preliminarily. A loop-mediated isothermal amplification method coupled with lateral flow biosensor (LAMP-LFB) assay, was developed and evaluated for detection of MTBC. Two sets of primers, which targeted IS and IS sequences of MTBC, were designed for establishment of multiplex LAMP-LFB assay. Read More

View Article and Full-Text PDF

Identifying Genomic Alterations in Small Cell Lung Cancer Using the Liquid Biopsy of Bronchial Washing Fluid.

Front Oncol 2021 26;11:647216. Epub 2021 Apr 26.

Department of Respiratory Ward One, Shanxi Provincial Cancer Hospital, Taiyuan, China.

With the rapid development of cancer genomics and immunomics, some new treatments of small cell lung cancer (SCLC) are emerging. However, there are limitations to the clinical use of tumor tissue. Our study aimed to evaluate the potential use of bronchial washing fluid (BWF) in the liquid biopsy of SCLC. Read More

View Article and Full-Text PDF

Amplicon-based RNAi construct targeting beta-C1 gene gives enhanced resistance against cotton leaf curl disease.

3 Biotech 2021 Jun 8;11(6):256. Epub 2021 May 8.

Molecular Virology and Gene Silencing Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Postal code 38000 Pakistan.

Cotton leaf curl disease (CLCuD) is one of the major limiting factors affecting cotton production in Pakistan for the last three decades. The disease is caused by begomoviruses of the family . RNA interference (RNAi) is a promising tool that has been proved effective against several pathogens. Read More

View Article and Full-Text PDF

Phenotypic, molecular and pathogenic characterization of infecting species in Rio de Janeiro, Brazil.

PeerJ 2021 27;9:e10782. Epub 2021 Apr 27.

Department of Agronomy, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.

Anthracnose is a disease caused by spp., one of the world's most damaging sweet and chili pepper pathogens, especially in tropical and subtropical regions. In the state of Rio de Janeiro, anthracnose is one of the main obstacles for pepper crops. Read More

View Article and Full-Text PDF

Mas Receptor Blockade Promotes Renal Vascular Response to Ang II after Partial Kidney Ischemia/Reperfusion in a Two-Kidney-One-Clip Hypertensive Rats Model.

Int J Nephrol 2021 20;2021:6618061. Epub 2021 Apr 20.

Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Partial kidney ischemia-reperfusion (IR) injury is the principal cause of acute kidney injury. The renin-angiotensin system (RAS) and hypertension also may be influenced by renal IR injury. In two models of partial renal IR with and without ischemia preconditioning (IPC) and using Mas receptor (MasR) blockade, A779 or its vehicle, the renal vascular responses to angiotensin II (Ang II) administration in two-kidney-one-clip (2K1C) hypertensive rats were determined. Read More

View Article and Full-Text PDF

Metagenomic analysis reveals oropharyngeal microbiota alterations in patients with COVID-19.

Signal Transduct Target Ther 2021 May 13;6(1):191. Epub 2021 May 13.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.

COVID-19 remains a serious emerging global health problem, and little is known about the role of oropharynx commensal microbes in infection susceptibility and severity. Here, we present the oropharyngeal microbiota characteristics identified by shotgun metagenomic sequencing analyses of oropharynx swab specimens from 31 COVID-19 patients, 29 influenza B patients, and 28 healthy controls. Our results revealed a distinct oropharyngeal microbiota composition in the COVID-19 patients, characterized by enrichment of opportunistic pathogens such as Veillonella and Megasphaera and depletion of Pseudopropionibacterium, Rothia, and Streptococcus. Read More

View Article and Full-Text PDF

Exploring genetic resistance to infectious salmon anaemia virus in Atlantic salmon by genome-wide association and RNA sequencing.

BMC Genomics 2021 May 13;22(1):345. Epub 2021 May 13.

The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK.

Background: Infectious Salmonid Anaemia Virus (ISAV) causes a notifiable disease that poses a large threat for Atlantic salmon (Salmo salar) aquaculture worldwide. There is no fully effective treatment or vaccine, and therefore selective breeding to increase resistance to ISAV is a promising avenue for disease prevention. Genomic selection and potentially genome editing can be applied to enhance host resistance, and these approaches benefit from improved knowledge of the genetic and functional basis of the target trait. Read More

View Article and Full-Text PDF

Antibiotics florfenicol and flumequine in the water column and sediments of Puyuhuapi Fjord, Chilean Patagonia.

Chemosphere 2021 Jul 24;275:130029. Epub 2021 Feb 24.

Departamento de Oceanografía and Centro de Investigación Oceanográfica COPAS Sur-Austral (PIA ANID), Universidad de Concepción, Concepción, Chile. Electronic address:

Chile is a major global producer of farmed salmon in the fjords of Patagonia, and therefore a major consumer of antibiotics. We tested whether the antibiotics florfenicol and flumequine persisted in the large Puyuhuapi Fjord after the six months following mandatory concerted treatment by all salmon farms present in the fjord. Antibiotics were detected in 26% of analyzed samples, but only within the particulate phase, with concentrations of florfenicol of up to 23. Read More

View Article and Full-Text PDF

Safety and Efficacy of GLP-1 Receptor Agonists in Children and Adolescents with Obesity: A Meta-Analysis.

J Pediatr 2021 May 10. Epub 2021 May 10.

Department of Paediatrics and Child Health, University College Cork, Cork, Ireland; Division of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, Philadelphia, USA; Perelman School of Medicine, University of Pennsylvania, Philadelphia.

Objectives: | To determine the weight, BMI, cardiometabolic and gastrointestinal effects of GLP-1 receptor agonists in children with obesity.

Study Design: | Web of Science, PubMed/MEDLINE, and Scopus databases from 01/01/1994-01/01/2021 for randomized control trials examining the weight, BMI, cardiometabolic or gastrointestinal effects of GLP-1 receptor agonists in children and adolescents with obesity. Data were extracted by two independent surveyors and a random effects model was applied to meta-analyze generic inverse variance outcomes. Read More

View Article and Full-Text PDF

Liquid Biopsy in Breast Cancer: Circulating Tumor Cells and Circulating Tumor DNA.

Authors:
Tae-Kyung Yoo

Adv Exp Med Biol 2021 ;1187:337-361

Department of Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

Cancer is associated with gene mutations, and the analysis of tumor-associated mutations is increasingly used for diagnostic, prognostic, and treatment purposes. These molecular landscapes of solid tumors are currently obtained from surgical or biopsy specimens. However, during cancer progression and treatment, selective pressures lead to additional genetic changes as tumors acquire drug resistance. Read More

View Article and Full-Text PDF
January 2021

Predicting Mycoplasma pneumoniae and Chlamydophila pneumoniae in community-acquired pneumonia (CAP) pneumonia: epidemiological study of respiratory tract infection using multiplex PCR assays.

Intern Emerg Med 2021 May 13. Epub 2021 May 13.

Department of Primary Care and Medical Education, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.

Community-acquired pneumonia (CAP) is a common illness that can lead to mortality. β-lactams are ineffective against atypical pathogen including Mycoplasma pneumoniae. We used molecular examinations to develop a decision tree to predict atypical pathogens with CAP and to examine the prevalence of macrolide resistance in Mycoplasma pneumoniae. Read More

View Article and Full-Text PDF

LY‑294002 enhances the chemosensitivity of liver cancer to oxaliplatin by blocking the PI3K/AKT/HIF‑1α pathway.

Mol Med Rep 2021 Jul 13;24(1). Epub 2021 May 13.

Medical School, Anhui University of Science and Technology, Huainan, Anhui 232001, P.R. China.

Liver cancer remains one of the leading causes of cancer deaths worldwide. The therapeutic effect of oxaliplatin on liver cancer is often limited by acquired resistance of the cancer cells. Abnormal activation of the PI3K/AKT pathway plays an important role in the acquired resistance of oxaliplatin. Read More

View Article and Full-Text PDF

The role of comprehensive analysis with circulating tumor DNA in advanced non-small cell lung cancer patients considered for osimertinib treatment.

Cancer Med 2021 May 12. Epub 2021 May 12.

Education and Research Center for Community Medicine, Faculty of Medicine, Saga University, Saga, Japan.

Background: EGFR mutations are good predictive markers of efficacy of EGFR tyrosine kinase inhibitors (EGFR-TKI), but whether comprehensive genomic analysis beyond EGFR itself with circulating tumor DNA (ctDNA) adds further predictive or prognostic value has not been clarified.

Methods: Patients with NSCLC who progressed after treatment with EGFR-TKI, and with EGFR T790 M detected by an approved companion diagnostic test (cobas ), were treated with osimertinib. Plasma samples were collected before and after treatment. Read More

View Article and Full-Text PDF

Optical genome mapping, a promising alternative to gold standard cytogenetic approaches in a series of acute lymphoblastic leukemias.

Genes Chromosomes Cancer 2021 May 13. Epub 2021 May 13.

CHU, Service d'Hématologie Biologique, Amiens, Picardie, France.

Acute lymphoblastic leukemias (ALL) are characterized by a large number of cytogenetic abnormalities of clinical interest that require the use of several complementary techniques. Optical genome mapping (OGM) is based on analysis of ultra-high molecular weight DNA molecules that provides a high-resolution genome-wide analysis highlighting copy number and structural anomalies, including balanced translocations. We compared OGM to standard techniques (karyotyping, fluorescent in situ hybridization, single nucleotide polymorphism-array and reverse transcription multiplex ligation-dependent probe amplification) in 10 selected B or T-ALL. Read More

View Article and Full-Text PDF

Circular RNA circPVT1 Contributes to Doxorubicin (DXR) Resistance of Osteosarcoma Cells by Regulating TRIAP1 via miR-137.

Biomed Res Int 2021 23;2021:7463867. Epub 2021 Apr 23.

Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun City, Jilin Province, China.

Background: Chemoresistance is a major obstacle to the treatment of osteosarcoma patients. Circular RNA (circRNA) circPVT1 has been reported to be related to the doxorubicin (DXR) resistance in osteosarcoma. This study is designed to explore the role and mechanism of circPVT1 in the DXR resistance of osteosarcoma. Read More

View Article and Full-Text PDF

Prevalence and Relatedness of -Mediated Colistin-Resistant Isolated From Livestock and Farmers in Japan.

Front Microbiol 2021 26;12:664931. Epub 2021 Apr 26.

Department of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan.

Colistin is used to treat infectious diseases in humans and livestock; it has also been used as a feed additive for livestock for approximately 50 years. Since the plasmid-mediated colistin resistance gene was discovered in China in 2015, it has been detected worldwide, mainly in livestock. In this study, we investigated the prevalence and characteristics of -mediated colistin-resistant in livestock and farmers in Japan. Read More

View Article and Full-Text PDF