53 results match your criteria residues 67-76

Effect of Organic Amendment on Mobility Behavior of Flupyradifurone in Two Different Indian Soils.

Bull Environ Contam Toxicol 2021 Apr 2. Epub 2021 Apr 2.

Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.

Flupyradifurone is a novel neonicotinoid insecticide, mainly used in okra in subtropical conditions for controlling whitefly and jassids. The present experiment was designed to generate information on the leaching behavior of flupyradifurone, 3-[(6-chloropyridin-3-yl)methyl-(2,2-difluroethyl)amino]-2H-furan-5-one, under different rainfall conditions by using packed soil columns. Under the continuous flow conditions, a significant quantity of flupyradifurone, 67. Read More

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Crystal structure of human LC8 bound to a peptide from Ebola virus VP35.

J Microbiol 2021 Apr 25;59(4):410-416. Epub 2021 Feb 25.

Disease Target Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.

Zaire ebolavirus, commonly called Ebola virus (EBOV), is an RNA virus that causes severe hemorrhagic fever with high mortality. Viral protein 35 (VP35) is a virulence factor encoded in the EBOV genome. VP35 inhibits host innate immune responses and functions as a critical cofactor for viral RNA replication. Read More

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Molecular and functional characterization of two malic enzymes from Leishmania parasites.

Mol Biochem Parasitol 2018 01 8;219:67-76. Epub 2017 Nov 8.

Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Instituto de Química y Fisicoquímica Biológica (IQUIFIB-CONICET), Junín 956, C1113AAD, Buenos Aires, Argentina. Electronic address:

Leishmania parasites cause a broad spectrum of clinical manifestations in humans and the available clinical treatments are far from satisfactory. Since these pathogens require large amounts of NADPH to maintain intracellular redox homeostasis, oxidoreductases that catalyze the production of NADPH are considered as potential drug targets against these diseases. In the sequenced genomes of most Leishmania spp. Read More

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January 2018

Structural diversity of lytic polysaccharide monooxygenases.

Curr Opin Struct Biol 2017 06 10;44:67-76. Epub 2017 Jan 10.

Department of Chemistry, Biotechnology, and Food Science, The Norwegian University of Life Sciences (NMBU), P.O. Box 5003, 1432 Ås, Norway.

Lytic polysaccharide monooxygenases (LPMOs) catalyze the oxidative cleavage of glycosidic bonds and represent a promising resource for development of industrial enzyme cocktails for biomass processing. LPMOs show high sequence and modular diversity and are known, so far, to cleave insoluble substrates such as cellulose, chitin and starch, as well as hemicelluloses such as beta-glucan, xyloglucan and xylan. All LPMOs share a catalytic histidine brace motif to bind copper, but differ strongly when it comes to the nature and arrangement of residues on the substrate-binding surface. Read More

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Sin Nombre hantavirus nucleocapsid protein exhibits a metal-dependent DNA-specific endonucleolytic activity.

Virology 2016 09 1;496:67-76. Epub 2016 Jun 1.

Institute of Medical Virology, Helmut-Ruska-Haus, Charité - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany. Electronic address:

We demonstrate that the nucleocapsid protein of Sin Nombre hantavirus (SNV-N) has a DNA-specific endonuclease activity. Upon incubation of SNV-N with DNA in the presence of magnesium or manganese, we observed DNA digestion in sequence-unspecific manner. In contrast, RNA was not affected under the same conditions. Read More

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September 2016

Effect of trastuzumab interchain disulfide bond cleavage on Fcγ receptor binding and antibody-dependent tumour cell phagocytosis.

J Biochem 2016 Jan 8;159(1):67-76. Epub 2015 Aug 8.

Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba 278-8510, Japan and.

The Fc domain of human IgG1 binds to Fcγ receptors (FcγRs) to induce effector functions such as phagocytosis. There are four interchain disulfide bonds between the H and L chains. In this study, the disulfide bonds within the IgG1 trastuzumab (TRA), which is specific for HER2, were cleaved by mild S-sulfonation or by mild reduction followed by S-alkylation with three different reagents. Read More

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January 2016

Peripheral group I metabotropic glutamate receptor activation leads to muscle mechanical hyperalgesia through TRPV1 phosphorylation in the rat.

J Pain 2015 Jan 1;16(1):67-76. Epub 2014 Nov 1.

Department of Neural and Pain Sciences, Program in Neuroscience, University of Maryland School of Dentistry, Baltimore, Maryland. Electronic address:

Unlabelled: Elevated glutamate levels within injured muscle play important roles in muscle pain and hyperalgesia. In this study, we hypothesized that protein kinase C (PKC)-dependent TRPV1 phosphorylation contributes to the muscle mechanical hyperalgesia following activation of Group I metabotropic glutamate receptors (mGlu1/5). Mechanical hyperalgesia induced by (R,S)-3,5-dihydroxyphenylglycine (DHPG), an mGlu1/5 agonist, in the masseter muscle was attenuated by AMG9810, a specific TRPV1 antagonist. Read More

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January 2015

Roles of the active site residues and metal cofactors in noncanonical base-pairing during catalysis by human DNA polymerase iota.

DNA Repair (Amst) 2014 Oct 9;22:67-76. Epub 2014 Aug 9.

Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia. Electronic address:

Human DNA polymerase iota (Pol ι) is a Y-family polymerase that can bypass various DNA lesions but possesses very low fidelity of DNA synthesis in vitro. Structural analysis of Pol ι revealed a narrow active site that promotes noncanonical base-pairing during catalysis. To better understand the structure-function relationships in the active site of Pol ι we investigated substitutions of individual amino acid residues in its fingers domain that contact either the templating or the incoming nucleotide. Read More

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October 2014

Sepp1(UF) forms are N-terminal selenoprotein P truncations that have peroxidase activity when coupled with thioredoxin reductase-1.

Free Radic Biol Med 2014 Apr 14;69:67-76. Epub 2014 Jan 14.

Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. Electronic address:

Mouse selenoprotein P (Sepp1) consists of an N-terminal domain (residues 1-239) that contains one selenocysteine (U) as residue 40 in a proposed redox-active motif (-UYLC-) and a C-terminal domain (residues 240-361) that contains nine selenocysteines. Sepp1 transports selenium from the liver to other tissues by receptor-mediated endocytosis. It also reduces oxidative stress in vivo by an unknown mechanism. Read More

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QM/MM study of catalytic mechanism of Xylanase Cex from Cellulomonas fimi.

J Mol Graph Model 2012 Jul 24;37:67-76. Epub 2012 Apr 24.

MOE Key Laboratory of Green Chemistry and Technology, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.

Xylanase Cex from Cellulomonas fimi is a bifunctional enzyme that catalyzes the degradation of both cellulose and xylan. As a result, it might find valuable applications in production of biofuels. In this work, we presented a detailed theoretical investigation of hydrolysis of the xylopentaose molecule catalyzed by Cex, using a hybrid quantum mechanical and molecular mechanical approach. Read More

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Heparan sulfate expression is affected by inflammatory stimuli in primary human endothelial cells.

Glycoconj J 2012 Jan 22;29(1):67-76. Epub 2011 Dec 22.

Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Blindern, Norway.

In diabetes the endothelium is either chronically or transiently exposed to hyperglycemic conditions. In addition, endothelial dysfunction in diabetes is related to changes in the inflammatory response and the turnover of extracellular matrix. This study was undertaken to study the effects of inflammatory stimuli on one particular matrix component, the heparan sulfate (HS) proteoglycans (PGs) synthesized by primary human umbilical cord vein endothelial cells (HUVEC). Read More

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January 2012

Synthesis of a covalent gemcitabine-(carbamate)-[anti-HER2/neu] immunochemotherapeutic and its cytotoxic anti-neoplastic activity against chemotherapeutic-resistant SKBr-3 mammary carcinoma.

Bioorg Med Chem 2011 Jan 25;19(1):67-76. Epub 2010 Nov 25.

Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, United States.

Unlabelled: Gemcitabine is a potent chemotherapeutic that exerts cytotoxic activity against several leukemias and a wide spectrum of carcinomas. A brief plasma half-life in part due to rapid deamination and chemotherapeutic-resistance frequently limit the utility of gemcitabine in clinical oncology. Selective 'targeted' delivery of gemcitabine represents a potential molecular strategy for simultaneously prolonging its plasma half-life and minimizing exposure of innocent tissues and organ systems. Read More

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January 2011

HCH and DDT Residues in Bivalves Anodonta woodiana from the Taihu Lake, China.

Arch Environ Contam Toxicol 2009 Jan 9;56(1):67-76. Epub 2008 May 9.

College of Fisheries, Nanjing Agriculture University, Wuxi 214081, China.

The present article attempts to use freshwater bivalves Anodonta woodiana for monitoring the pollution of hexachlorocyclohexane isomers (alpha-, beta-, gamma-, delta-HCH), dichlorodiphenyltrichloroethanes (o, p', p, p'-DDT) and metabolites (p, p'-DDE, p, p'-DDD) in the Taihu Lake, China. A total of 36 bivalves were sampled from 4 sites of Huzhou city, Dapu of Yixing city, Xueyan of Changzhou city, and Wulihu of Wuxi city around the lake in August-October 2004. The organochlorines were detected in all bivalves, and the mean concentration of SigmaDDTs (7. Read More

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January 2009

Characterization and prediction of protein interfaces to infer protein-protein interaction networks.

Curr Pharm Biotechnol 2008 Apr;9(2):67-76

Koc University, Center for Computational Biology and Bioinformatics, and College of Engineering, Rumeli Feneri Yolu, 34450 Sariyer Istanbul, Turkey.

Complex protein-protein interaction networks govern biological processes in cells. Protein interfaces are the sites where proteins physically interact. Identification and characterization of protein interfaces will lead to understanding how proteins interact with each other and how they are involved in protein-protein interaction networks. Read More

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Piezoelectric quartz crystal sensor for rapid analysis of pirimicarb residues using molecularly imprinted polymers as recognition elements.

Anal Chim Acta 2006 Aug 5;576(1):67-76. Epub 2006 May 5.

Department of Chemistry, The University of Hong Kong, Hong Kong SAR, China.

A new piezoelectric quartz crystal (PQC) sensor using molecularly imprinted polymer (MIP) as sensing material has been developed for fast and onsite determination of pirimicarb in contaminated vegetables. Three MIPs particles have been prepared by conventional bulk polymerization (MIP-B) and precipitation polymerization in either acetonitrile (MIP-P1) or chloroform (MIP-P2). MIP-P2, with uniform spherical shape and mean diameter at about 50 nm, has shown the best performance as the sensing material for PQC sensor. Read More

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CgNa, a type I toxin from the giant Caribbean sea anemone Condylactis gigantea shows structural similarities to both type I and II toxins, as well as distinctive structural and functional properties(1).

Biochem J 2007 Aug;406(1):67-76

Instituto de Fisiología, Universidad Autónoma de Puebla, 14 Sur 6301, 72570 Puebla, México.

CgNa (Condylactis gigantea neurotoxin) is a 47-amino-acid- residue toxin from the giant Caribbean sea anemone Condylactis gigantea. The structure of CgNa, which was solved by 1H-NMR spectroscopy, is somewhat atypical and displays significant homology with both type I and II anemone toxins. CgNa also displays a considerable number of exceptions to the canonical structural elements that are thought to be essential for the activity of this group of toxins. Read More

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Dissipation of sulfosulfuron in water - bioaccumulation of residues in fish - LC-MS/MS-ESI identification and quantification of metabolites.

Chemosphere 2007 Jun 7;68(3):495-500. Epub 2007 Feb 7.

Department of Analytical Chemistry, International Institute of Bio-technology and Toxicology (IIBAT), Padappai, Chennai 601 301, Tamil Nadu, India.

Dissipation study of sulfosulfuron in natural water and its bioaccumulation in fish was conducted at 25+/-2 degrees C and at two different concentration levels 1mgl(-1) and 2mgl(-1). The dissipation data in water showed the DT50 and DT90 values 67-76 and 222-253 days and followed first order kinetics. Bioaccumulation of sulfosulfuron in fish was conducted under static conditions exposing the fish at one-tenth of sub-lethal concentration 9mgl(-1) and at double the concentration 18mgl(-1), for a period of 56 days. Read More

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Phase behavior and the partitioning of caveolin-1 scaffolding domain peptides in model lipid bilayers.

J Colloid Interface Sci 2006 Dec 4;304(1):67-76. Epub 2006 Oct 4.

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

The membrane binding and model lipid raft interaction of synthetic peptides derived from the caveolin scaffolding domain (CSD) of the protein caveolin-1 have been investigated. CSD peptides bind preferentially to liquid-disordered domains in model lipid bilayers composed of cholesterol and an equimolar ratio of dioleoylphosphatidylcholine (DOPC) and brain sphingomyelin. Three caveolin-1 peptides were studied: the scaffolding domain (residues 83-101), a water-insoluble construct containing residues 89-101, and a water-soluble construct containing residues 89-101. Read More

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December 2006

Location of the cell-binding domain of CP65, a 65kDa cysteine proteinase involved in Trichomonas vaginalis cytotoxicity.

Int J Biochem Cell Biol 2006 3;38(12):2114-27. Epub 2006 Jul 3.

Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Av. IPN 2508, Col. San Pedro Zacatenco, CP 07360, Mexico City, Mexico.

The cysteine proteinase (CP) of 65kDa, CP65, binds to the surface of HeLa cells and is involved in Trichomonas vaginalis cellular damage. To identify and locate the CP65 cellular-binding domain, we enriched the CP65 protein band by ammonium sulfate fractionation and ion-exchange chromatography and the N-terminal sequence was obtained. A 618bp gene fragment was obtained by PCR using genomic DNA as template and primers derived from the N-terminal sequence of CP65 and the Asn papain-catalytic conserved region. Read More

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December 2006

Demonstration of N- and C-terminal domain intramolecular interactions in rat liver carnitine palmitoyltransferase 1 that determine its degree of malonyl-CoA sensitivity.

Biochem J 2005 Apr;387(Pt 1):67-76

Département d'Endocrinologie, Institut Cochin, INSERM U567, CNRS Unité Mixte de Recherche 8104, Université René Descartes, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France.

We have previously proposed that changes in malonyl-CoA sensitivity of rat L-CPT1 (liver carnitine palmitoyltransferase 1) might occur through modulation of interactions between its cytosolic N- and C-terminal domains. By using a cross-linking strategy based on the trypsin-resistant folded state of L-CPT1, we have now shown the existence of such N-C (N- and C-terminal domain) intramolecular interactions both in wild-type L-CPT1 expressed in Saccharomyces cerevisiae and in the native L-CPT1 in fed rat liver mitochondria. These N-C intramolecular interactions were found to be either totally (48-h starvation) or partially abolished (streptozotocin-induced diabetes) in mitochondria isolated from animals in which the enzyme displays decreased malonyl-CoA sensitivity. Read More

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Study of peptides containing modified lysine residues by tandem mass spectrometry: precursor ion scanning of hexanal-modified peptides.

Rapid Commun Mass Spectrom 2004 ;18(1):67-76

Nestlé Research Center, Nestec Ltd., Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland.

Upon hexanal-modification in the presence of NaCNBH(3), the oxidized B chain of insulin becomes mono- and further dialkylated on both the N-terminal and Lys(29) residues. A pseudo-MS(3) study was performed with a triple-quadrupole mass spectrometer on the different modified lysine-containing species to gain further insights into the characteristic fragmentation pattern. These fragmentations, in good agreement with true MS(3) measurements obtained using an ion trap mass spectrometer, highlighted characteristic monoalkylated lysine (immonium-NH(3)) and protonated modified caprolactam ions at m/z 168 and 213, respectively. Read More

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Identification of canine alpha-lactalbumin.

J Vet Med Sci 2000 Nov;62(11):1217-9

Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Gakuen KibanaDai, Japan.

The nucleotide sequence of canine alpha-lactalbumin cDNA from canine mammary tissue was determined by polymerase chain reaction with degenerate primers. A 742 base pairs nucleotide sequence cloned was similar to the size of mRNA in Northern blot analysis. The cDNA encodes 142 amino acid residues containing the conserved sequence motif of alpha-lactalbumin, demonstrating the highest homology with pig (73% identity-82% similarity) among the known amino acid sequences of alpha-lactalbumin. Read More

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November 2000

Shared structural motifs in TCR of glycopeptide-recognizing T cell hybridomas.

Eur J Immunol 1999 09;29(9):2759-68

Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark.

The TCR structure of T cell hybridomas recognizing a tumor glycan-defined epitope has been studied using reverse transcriptase-PCR and gene sequencing. The hybridomas had been raised against a glycopeptide, T72(Tn), consisting of the mouse hemoglobin-derived decapeptide Hb(67 - 76), O-glycoslated in position 72 with alpha-D-GalNAc. The glycan-specific hybridomas varied widely in their use of Valpha genes although Valpha4 was predominant, being present in one third of them. Read More

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September 1999

Selective isopeptide bond formation: coupling ubiquitin(67-76) with histone 2A(114-128) by use of the transfer active ester condensation technique.

J Pept Res 1999 Jun;53(6):673-7

Edinburgh Centre for Protein Technology, Department of Chemistry, University of Edinburgh, UK.

A peptide, ubiquitin(67-76)-histone 2A(114-128) fragment (UBH2AF), was synthesized by selective formation of an isopeptide bond between the C-terminus of ubiquitin(67-76) and the epsilon-amino group of lysine-119 in histone 2A(114-128) which contained 4 lysine residues at positions 118, 119, 125 and 127, respectively. The transfer active ester condensation technique, together with the Tnm amine protecting group, were used successfully in the peptide segment coupling reaction. [structure: see text] Read More

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A single amino acid in the second transmembrane domain of GABA rho subunits is a determinant of the response kinetics of GABAC receptors.

J Neurobiol 1999 Jul;40(1):67-76

Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, 1855 West Taylor Street, Chicago, Illinois 60612, USA.

The rho subunits that constitute the gamma-aminobutyric acid (GABA)C receptors of retinal neurons form a unique subclass of ligand-gated chloride channels that give rise to sustained GABA-evoked currents that exhibit slow offset (deactivation) kinetics. We exploited this property to examine the molecular mechanisms that govern the disparate response kinetics and pharmacology of perch GABA rho1B and rho2A subunits expressed in Xenopus oocytes. Using a combination of domain swapping and site-directed mutagenesis, we identified the residues at amino acid position 320 in the second transmembrane domain as an important determinant of the receptor kinetics of GABAC receptors. Read More

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RNA expression from a site-specific non-LTR retrotransposon microinjected into Xenopus oocytes.

Genetica 1998 ;104(1):67-76

Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132, USA.

Tx1L is a site-specific non-LTR retrotransposon (NLR) that has been identified in the genome of Xenopus laevis. Using microinjection into Xenopus oocytes, several aspects of RNA expression by these elements were investigated. With constructs carrying various parts of the element we saw no evidence of promoter activity, unlike what has been shown for several other elements of this class. Read More

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February 1999

Lumbricin I, a novel proline-rich antimicrobial peptide from the earthworm: purification, cDNA cloning and molecular characterization.

Biochim Biophys Acta 1998 Oct;1408(1):67-76

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Yusong-gu Kusong-dong, Taejon 305-701, South Korea.

A novel antimicrobial peptide was isolated and characterized from the earthworm, Lumbricus rubellus. The antimicrobial peptide was purified to homogeneity by a heparin-affinity column and C18 reverse-phase HPLC, and named lumbricin I. Lumbricin I was a proline-rich antimicrobial peptide of 62 amino acids (15% proline in molar ratio; molecular mass, 7231 Da), whose complete sequence was determined by a combination of peptide sequence and cDNA analysis. Read More

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October 1998

Anatomy of the antigenic structure of a large membrane autoantigen, the muscle-type nicotinic acetylcholine receptor.

Immunol Rev 1998 Jun;163:89-120

Department of Biochemistry, Hellenic Pasteur Institute, Athens, Greece.

The neuromuscular junction nicotinic acetylcholine receptor (AChR), a pentameric membrane glycoprotein, is the autoantigen involved in the autoimmune disease myasthenia gravis (MG). In animals immunized with intact AChR and in human MG, the anti-AChR antibody response is polyclonal. However, a small extracellular region of the AChR alpha-subunit, the main immunogenic region (MIR), seems to be a major target for anti-AChR antibodies. Read More

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Molecular cloning and characterization of meichroacidin (male meiotic metaphase chromosome-associated acidic protein).

Dev Biol 1998 May;197(1):67-76

Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, 565, Japan.

We have isolated a cDNA clone encoding a germ cell specific protein from an expression cDNA library prepared from the mouse testis, using testis-specific polyclonal antibodies. Sequence analysis of the cDNA revealed that the deduced amino acid sequence consisted of 284 residues, including a nominal repeat structure in the N-terminal region. Northern blot analysis revealed the presence of a transcript of 1. Read More

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The isolation of parvalbumin isoforms from the tail muscle of the American alligator (Alligator mississipiensis).

J Inorg Biochem 1997 Apr;66(1):67-76

Gustaf H. Carlson School of Chemistry, Clark University, Worcester, MA 01610, USA.

Multiple parvalbumin isoforms have been detected in the tail (skeletal) muscle of the American alligator (Alligator mississipiensis). One of these isoforms (APV-1) has been highly purified and partially characterized. Protein purification involved mainly gel filtration and anion exchange chromatography, and characterization included gel electrophoresis, amino acid composition analysis, metal ion analysis, MALDI-TOF and ESI mass spectrometry, ultraviolet and fluorescence spectroscopy, and one- and two-dimensional 500 MHz proton NMR spectroscopy. Read More

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