245 results match your criteria reside quiescent

Metabolomic Alteration of Oral Keratinocytes and Fibroblasts in Hypoxia.

J Clin Med 2021 Mar 10;10(6). Epub 2021 Mar 10.

Division of Biomimetics, School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan.

The oxygen concentration in normal human tissue under physiologic conditions is lower than the atmospheric oxygen concentration. The more hypoxic condition has been observed in the cells with wound healing and cancer. Somatic stem cells reside in a hypoxic microenvironment in vivo and prefer hypoxic culture conditions in vitro. Read More

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Phenotypic diversity and metabolic specialization of renal endothelial cells.

Nat Rev Nephrol 2021 Mar 25. Epub 2021 Mar 25.

Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology, KU Leuven and Center for Cancer Biology, VIB, Leuven, Belgium.

Complex multicellular life in mammals relies on functional cooperation of different organs for the survival of the whole organism. The kidneys play a critical part in this process through the maintenance of fluid volume and composition homeostasis, which enables other organs to fulfil their tasks. The renal endothelium exhibits phenotypic and molecular traits that distinguish it from endothelia of other organs. Read More

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Modular Approaches to Understand the Immunobiology of Human Immunodeficiency Virus Latency.

Viral Immunol 2021 Feb 17. Epub 2021 Feb 17.

Department of Microbiology & Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA.

Despite advances in slowing the progression of acquired immunodeficiency syndrome (AIDS), there is no viable cure for human immunodeficiency virus (HIV). The challenge toward a cure is mainly the formation and maintenance of a latent reservoir of cells that harbor the virus in both replication-competent and replication-defective states. This small niche of quiescent cells has been identified to reside primarily in quiescent and memory CD4 T cells, but parameters that could reliably distinguish an infected T cell from an uninfected one, if any, are not clear. Read More

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February 2021

Does coronaviruses induce neurodegenerative diseases? A systematic review on the neurotropism and neuroinvasion of SARS-CoV-2.

Drug Discov Ther 2021 Jan 31;14(6):262-272. Epub 2020 Dec 31.

Institut Pasteur de Tunis, Laboratoire des Biomolécules, Venins et Applications Théranostiques, Tunis, Tunisia.

The novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was identified in 2019 in Wuhan, China. Clinically, respiratory tract symptoms as well as other organs disorders are observed in patients positively diagnosed coronavirus disease 2019 (COVID-19). In addition, neurological symptoms, mainly anosmia, ageusia and headache were observed in many patients. Read More

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January 2021

Chromatin structure restricts origin utilization when quiescent cells re-enter the cell cycle.

Nucleic Acids Res 2021 01;49(2):864-878

Department of Molecular Genetics and Microbiology University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA.

Quiescent cells reside in G0 phase, which is characterized by the absence of cell growth and proliferation. These cells remain viable and re-enter the cell cycle when prompted by appropriate signals. Using a budding yeast model of cellular quiescence, we investigated the program that initiated DNA replication when these G0 cells resumed growth. Read More

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January 2021

Phagocytosis by the Retinal Pigment Epithelium: Recognition, Resolution, Recycling.

Front Immunol 2020 13;11:604205. Epub 2020 Nov 13.

Program in Cell Biology, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children, Toronto, ON, Canada.

Tissue-resident phagocytes are responsible for the routine binding, engulfment, and resolution of their meals. Such populations of cells express appropriate surface receptors that are tailored to recognize the phagocytic targets of their niche and initiate the actin polymerization that drives internalization. Tissue-resident phagocytes also harbor enzymes and transporters along the endocytic pathway that orchestrate the resolution of ingested macromolecules from the phagolysosome. Read More

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November 2020

High-resolution mouse subventricular zone stem-cell niche transcriptome reveals features of lineage, anatomy, and aging.

Proc Natl Acad Sci U S A 2020 12 23;117(49):31448-31458. Epub 2020 Nov 23.

Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065;

Adult neural stem cells (NSC) serve as a reservoir for brain plasticity and origin for certain gliomas. Lineage tracing and genomic approaches have portrayed complex underlying heterogeneity within the major anatomical location for NSC, the subventricular zone (SVZ). To gain a comprehensive profile of NSC heterogeneity, we utilized a well-validated stem/progenitor-specific reporter transgene in concert with single-cell RNA sequencing to achieve unbiased analysis of SVZ cells from infancy to advanced age. Read More

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December 2020

Cellular Mechanisms and Regulation of Quiescence.

Dev Cell 2020 11;55(3):259-271

Whitehead Institute for Biomedical Research, 455 Main Street, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. Electronic address:

Quiescence is a state of reversible proliferative arrest in which cells are not actively dividing and yet retain the capacity to reenter the cell cycle upon receiving an appropriate stimulus. Quiescent cells are remarkably diverse-they reside in different locations throughout the body, serve distinct roles, and are activated by a variety of signals. Despite this diversity, all quiescent cells must be able to persist in a nondividing state without compromising their proliferative potential, which requires changes to core cellular programs. Read More

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November 2020

Intestinal epithelial plasticity and regeneration via cell dedifferentiation.

Cell Regen 2020 Sep 1;9(1):14. Epub 2020 Sep 1.

The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

The intestinal epithelium possesses a great capacity of self-renewal under normal homeostatic conditions and of regeneration upon damages. The renewal and regenerative processes are driven by intestinal stem cells (ISCs), which reside at the base of crypts and are marked by Lgr5. As Lgr5 ISCs undergo fast cycling and are vulnerable to damages, there must be other types of cells that can replenish the lost Lgr5 ISCs and then regenerate the damage epithelium. Read More

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September 2020

Resolving Fates and Single-Cell Transcriptomes of Hematopoietic Stem Cell Clones by PolyloxExpress Barcoding.

Cell Stem Cell 2020 09 11;27(3):383-395.e8. Epub 2020 Aug 11.

Division of Cellular Immunology, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. Electronic address:

Lineage tracing reveals hematopoietic stem cell (HSC) fates, while single-cell RNA sequencing identifies snapshots of HSC transcriptomes. To obtain information on fate plus transcriptome in the same cell, we developed the PolyloxExpress allele, enabling Cre-recombinase-dependent RNA barcoding in situ. Linking fates to single HSC transcriptomes provided the information required to identify transcriptional signatures of HSC fates, which were not apparent in single-HSC transcriptomes alone. Read More

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September 2020

Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation.

Genome Biol 2020 07 1;21(1):157. Epub 2020 Jul 1.

Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, Cambridge, UK.

Background: Haematopoietic stem cells (HSCs) first arise during development in the aorta-gonad-mesonephros (AGM) region of the embryo from a population of haemogenic endothelial cells which undergo endothelial-to-haematopoietic transition (EHT). Despite the progress achieved in recent years, the molecular mechanisms driving EHT are still poorly understood, especially in human where the AGM region is not easily accessible.

Results: In this study, we take advantage of a human pluripotent stem cell (hPSC) differentiation system and single-cell transcriptomics to recapitulate EHT in vitro and uncover mechanisms by which the haemogenic endothelium generates early haematopoietic cells. Read More

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Connecting secretome to hematopoietic stem cell phenotype shifts in an engineered bone marrow niche.

Integr Biol (Camb) 2020 07;12(7):175-187

Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Hematopoietic stem cells (HSCs) primarily reside in the bone marrow, where they receive external cues from their local microenvironment. The complex milieu of biophysical cues, cellular components and cell-secreted factors regulates the process by which HSC produce the blood and immune system. We previously showed direct coculture of primary murine hematopoietic stem and progenitor cells with a population of marrow-derived mesenchymal stromal and progenitor cells (MSPCs) in a methacrylamide-functionalized gelatin (GelMA) hydrogel improves hematopoietic progenitor maintenance. Read More

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Oxidative resistance of leukemic stem cells and oxidative damage to hematopoietic stem cells under pro-oxidative therapy.

Cell Death Dis 2020 04 27;11(4):291. Epub 2020 Apr 27.

Department of Basic Medical Sciences, Medical College of Taizhou University, Taizhou, 318000, Zhejiang, China.

Leukemic stem cells (LSCs) and hematopoietic stem cells (HSCs) are both dependent on the hypoxic bone marrow (BM) microenvironment (also known as the BM niche). There is always fierce competition between the two types of cells, and the former exhibits a greater competitive advantage than the latter via multiple mechanisms. Under hypoxia, the dynamic balance between the generation and clearing of intracellular reactive oxygen species (ROS) is conducive to maintaining a quiescent state of cells. Read More

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Quiescent Neural Stem Cells for Brain Repair and Regeneration: Lessons from Model Systems.

Trends Neurosci 2020 04 4;43(4):213-226. Epub 2020 Mar 4.

The Gurdon Institute and Department of Physiology, Development, and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK. Electronic address:

Neural stem cells (NSCs) are multipotent progenitors that are responsible for producing all of the neurons and macroglia in the nervous system. In adult mammals, NSCs reside predominantly in a mitotically dormant, quiescent state, but they can proliferate in response to environmental inputs such as feeding or exercise. It is hoped that quiescent NSCs could be activated therapeutically to contribute towards repair in humans. Read More

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Satellite cells and their regulation in livestock.

J Anim Sci 2020 May;98(5)

Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA.

Satellite cells are the myogenic stem and progenitor population found in skeletal muscle. These cells typically reside in a quiescent state until called upon to support repair, regeneration, or muscle growth. The activities of satellite cells are orchestrated by systemic hormones, autocrine and paracrine growth factors, and the composition of the basal lamina of the muscle fiber. Read More

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Stem Cell Aging in Skeletal Muscle Regeneration and Disease.

Int J Mol Sci 2020 Mar 6;21(5). Epub 2020 Mar 6.

Department of Cardiology Keio University School of Medicine, Tokyo 160-8582, Japan.

Skeletal muscle comprises 30-40% of the weight of a healthy human body and is required for voluntary movements in humans. Mature skeletal muscle is formed by multinuclear cells, which are called myofibers. Formation of myofibers depends on the proliferation, differentiation, and fusion of muscle progenitor cells during development and after injury. Read More

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Regeneration competent satellite cell niches in rat engineered skeletal muscle.

FASEB Bioadv 2019 Dec 13;1(12):731-746. Epub 2019 Nov 13.

Institute of Pharmacology and Toxicology Georg-August University Göttingen Göttingen Germany.

Satellite cells reside in defined niches and are activated upon skeletal muscle injury to facilitate regeneration. Mechanistic studies of skeletal muscle regeneration are hampered by the inability to faithfully simulate satellite cell biology in vitro. We sought to overcome this limitation by developing tissue engineered skeletal muscle (ESM) with (1) satellite cell niches and (2) the capacity to regenerate after injury. Read More

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December 2019

Adult Human Glioblastomas Harbor Radial Glia-like Cells.

Stem Cell Reports 2020 02 30;14(2):338-350. Epub 2020 Jan 30.

Department of Neurosurgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address:

Radial glia (RG) cells are the first neural stem cells to appear during embryonic development. Adult human glioblastomas harbor a subpopulation of RG-like cells with typical RG morphology and markers. The cells exhibit the classic and unique mitotic behavior of normal RG in a cell-autonomous manner. Read More

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February 2020

Adult Muscle Stem Cells: Exploring the Links Between Systemic and Cellular Metabolism.

Front Cell Dev Biol 2019 6;7:312. Epub 2019 Dec 6.

Centre for Cellular and Molecular Biology, Hyderabad, India.

Emerging evidence suggests that metabolites are important regulators of skeletal muscle stem cell (MuSC) function and fate. While highly proliferative in early life, MuSCs reside in adult skeletal muscle tissue in a quiescent and metabolically depressed state, but are critical for the homeostatic maintenance and regenerative response of the tissue to damage. It is well established that metabolic activity in MuSC changes with their functional activation, but the spatiotemporal links between physiological metabolism and stem cell metabolism require explicit delineation. Read More

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December 2019

Proteomic analyses of decellularized porcine ovaries identified new matrisome proteins and spatial differences across and within ovarian compartments.

Sci Rep 2019 12 27;9(1):20001. Epub 2019 Dec 27.

Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, USA.

Premature ovarian insufficiency (POI) affects approximately 1% of women. We aim to understand the ovarian microenvironment, including the extracellular matrix (ECM) and associated proteins (matrisome), and its role in controlling folliculogenesis. We mapped the composition of the matrisome of porcine ovaries through the cortical compartment, where quiescent follicles reside and the medullary compartment, where the larger follicles grow and mature. Read More

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December 2019

Alternative polyadenylation of Pax3 controls muscle stem cell fate and muscle function.

Science 2019 11;366(6466):734-738

Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA.

Adult stem cells are essential for tissue homeostasis. In skeletal muscle, muscle stem cells (MuSCs) reside in a quiescent state, but little is known about the mechanisms that control homeostatic turnover. Here we show that, in mice, the variation in MuSC activation rate among different muscles (for example, limb versus diaphragm muscles) is determined by the levels of the transcription factor Pax3. Read More

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November 2019

Haematopoietic stem cells in perisinusoidal niches are protected from ageing.

Nat Cell Biol 2019 11 4;21(11):1309-1320. Epub 2019 Nov 4.

Institute of Molecular Medicine, Stem Cells and Aging, Aging Research Center, Ulm University, Ulm, Germany.

With ageing, intrinsic haematopoietic stem cell (HSC) activity decreases, resulting in impaired tissue homeostasis, reduced engraftment following transplantation and increased susceptibility to diseases. However, whether ageing also affects the HSC niche, and thereby impairs its capacity to support HSC function, is still widely debated. Here, by using in-vivo long-term label-retention assays we demonstrate that aged label-retaining HSCs, which are, in old mice, the most quiescent HSC subpopulation with the highest regenerative capacity and cellular polarity, reside predominantly in perisinusoidal niches. Read More

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November 2019

Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice.

PLoS Genet 2019 10 18;15(10):e1008408. Epub 2019 Oct 18.

Institute of Biochemistry and Cell Biology (IBBC), National Research Council (CNR), Rome, Italy.

Satellite cells (SCs) are muscle stem cells that remain quiescent during homeostasis and are activated in response to acute muscle damage or in chronic degenerative conditions such as Duchenne Muscular Dystrophy. The activity of SCs is supported by specialized cells which either reside in the muscle or are recruited in regenerating skeletal muscles, such as for instance macrophages (MΦs). By using a dystrophic mouse model of transient MΦ depletion, we describe a shift in identity of muscle stem cells dependent on the crosstalk between MΦs and SCs. Read More

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October 2019

Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.

Biomaterials 2019 12 8;225:119539. Epub 2019 Oct 8.

Key Laboratory of Experimental Teratology, Ministry of Education Department of Medical Genetics, School of Basic Medical Sciences, Shandong University Jinan, Shandong, 250012, China; Advanced Medical Research Institute, Shandong University Jinan, Shandong, 250012, China. Electronic address:

Photostimulation has been widely used in neuromodulation. However, existing optogenetics techniques require genetic alternation of the targeted cell or tissue. Here, we report that neural stem cells (NSCs) constitutionally express blue/red light-sensitive photoreceptors. Read More

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December 2019

BCR-ABL Independent Mechanisms of Resistance in Chronic Myeloid Leukemia.

Front Oncol 2019 24;9:939. Epub 2019 Sep 24.

Haematology and Haematopoietic Stem Cell Transplant Center, AORMN Hospital, Pesaro, Italy.

Not all chronic myeloid leukemia (CML) patients are cured with tyrosine kinase inhibitors (TKIs), and a proportion of them develop resistance. Recently, continuous BCR-ABL gene expression has been found in resistant cells with undetectable BCR-ABL protein expression, indicating that resistance may occur through kinase independent mechanisms, mainly due to the persistence of leukemia stem cells (LSCs). LSCs reside in the bone marrow niche in a quiescent state, and are characterized by a high heterogeneity in genetic, epigenetic, and transcriptional mechanisms. Read More

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September 2019

ATR kinase inhibition sensitizes quiescent human cells to the lethal effects of cisplatin but increases mutagenesis.

Mutat Res 2019 11 17;816-818:111678. Epub 2019 Sep 17.

Department of Pharmacology and Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Hwy, Dayton, OH, 45435, United States. Electronic address:

The ATR protein kinase is known to protect cells from DNA damage induced during the replicative phase of the cell cycle. Small molecule ATR kinase inhibitors have therefore been developed to improve the effectiveness of DNA damage-based chemotherapy regimens aimed at killing rapidly proliferating tumor cells. However, whether ATR functions in a similar manner in non-replicating cells has not been examined and is important considering the fact that most cells in the body, including cancer stem cells in solid tumors, normally reside in either a quiescent or differentiated non-replicating state. Read More

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November 2019

Soluble Signals and Remodeling in a Synthetic Gelatin-Based Hematopoietic Stem Cell Niche.

Adv Healthc Mater 2019 10 18;8(20):e1900751. Epub 2019 Sep 18.

Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Hematopoietic stem cells (HSCs) reside in the bone marrow within niches that provide microenvironmental signals in the form of biophysical cues, bound and diffusible biomolecules, and heterotypic cell-cell interactions that influence HSC fate decisions. This study seeks to inform the development of a synthetic culture platform that promotes ex vivo HSC expansion without exhaustion. A library of methacrylamide-functionalized gelatin (GelMA) hydrogels is used to explore remodeling and crosstalk from mesenchymal stromal cells (MSCs) on the expansion and quiescence of murine HSCs. Read More

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October 2019

Wnt4 from the Niche Controls the Mechano-Properties and Quiescent State of Muscle Stem Cells.

Cell Stem Cell 2019 11 5;25(5):654-665.e4. Epub 2019 Sep 5.

The Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Department of Orthopaedic Surgery, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address:

Satellite cells (SCs) reside in a dormant state during tissue homeostasis. The specific paracrine agents and niche cells that maintain SC quiescence remain unknown. We find that Wnt4 produced by the muscle fiber maintains SC quiescence through RhoA. Read More

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November 2019

Bcl2-Expressing Quiescent Type B Neural Stem Cells in the Ventricular-Subventricular Zone Are Resistant to Concurrent Temozolomide/X-Irradiation.

Stem Cells 2019 12 17;37(12):1629-1639. Epub 2019 Oct 17.

Department of Radiation Oncology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.

The ventricular-subventricular zone (V-SVZ) of the mammalian brain is a site of adult neurogenesis. Within the V-SVZ reside type B neural stem cells (NSCs) and type A neuroblasts. The V-SVZ is also a primary site for very aggressive glioblastoma (GBM). Read More

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December 2019

Simultaneous Ribosome Profiling of Human Host Cells Infected with Toxoplasma gondii.

mSphere 2019 06 5;4(3). Epub 2019 Jun 5.

Department of Pharmacology & Toxicology, Indiana University School of Medicine, Indianapolis, Indiana, USA

is a ubiquitous obligate intracellular parasite that infects the nucleated cells of warm-blooded animals. From within the parasitophorous vacuole in which they reside, tachyzoites secrete an arsenal of effector proteins that can reprogram host gene expression to facilitate parasite survival and replication. Gaining a better understanding of how host gene expression is altered upon infection is central for understanding parasite strategies for host invasion and for developing new parasite therapies. Read More

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