190,309 results match your criteria reproductive technology


Antibacterial effects of nano-decoction iron polysulfide in epididymitis and the systematic evaluation of its toxicity on the reproductive health of male mice.

Ecotoxicol Environ Saf 2022 Jan 12;231:113184. Epub 2022 Jan 12.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, China; Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 211166, China; The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address:

Ferrous iron and polysulfide (Fe(II)S aq) is a nano-decoction. It is usually prepared from the suspension of iron sulfide nanomaterial, using autoclave and centrifugation. A previous study conducted in our laboratory revealed that Fe(II)S aq was highly antibacterial, and it could efficiently kill more than 90% population of Escherichia coli and Staphylococcus aureus, within 5 min of the treatment. Read More

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January 2022

Single-cell Transcriptomic Profiling of the Hypothalamic Median Eminence during Aging.

J Genet Genomics 2022 Jan 12. Epub 2022 Jan 12.

State Key Laboratory of Molecular Development Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100101, China; Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Beijing 100101, China; Chinese Institute for Brain Research, Beijing 102206, China; Beijing Children's Hospital, Capital Medical University, Beijing 100045, China. Electronic address:

Aging is a slow and progressive natural process that compromises the normal functions of cells, tissues, organs and systems. The aging of the hypothalamic median eminence (ME), a structural gate linking neural and endocrine systems, may impair hormone release, energy homeostasis and central sensing of circulating molecules, leading to systemic and reproductive aging. However, the molecular and cellular features of ME aging remain largely unknown. Read More

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January 2022

Installing oncofertility programs for breast cancer in limited versus optimum resource settings: Empirical data from 39 surveyed centers in Repro-Can-OPEN Study Part I & II.

J Assist Reprod Genet 2022 Jan 15. Epub 2022 Jan 15.

Oncofertility Consortium, Michigan State University, 965 Wilson Road, Room A626B, East Lansing, MI, 48824-1316, USA.

Purpose: As a further step to elucidate the actual diverse spectrum of oncofertility practices for breast cancer around the globe, we present and discuss the comparisons of oncofertility practices for breast cancer in limited versus optimum resource settings based on data collected in the Repro-Can-OPEN Study Part I & II.

Methods: We surveyed 39 oncofertility centers including 14 in limited resource settings from Africa, Asia & Latin America (Repro-Can-OPEN Study Part I), and 25 in optimum resource settings from the United States, Europe, Australia and Japan (Repro-Can-OPEN Study Part II). Survey questions covered the availability of fertility preservation and restoration options offered to young female patients with breast cancer as well as the degree of utilization. Read More

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January 2022

Genetic and molecular Omp25 analyses from worldwide Brucella canis strains: possible mutational influences in protein function.

Gene 2022 Jan 11:146175. Epub 2022 Jan 11.

Laboratory of Veterinary Bacteriology, Veterinary School, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Ciências Veterinárias, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. Electronic address:

Brucella canis is responsible for canine brucellosis, a neglected zoonotic disease. The omp25 gene has been described as an important marker for Brucella intra-species differentiation, in addition to the ability to interact with the host immune system. Therefore, this study investigated the omp25 sequence from B. Read More

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January 2022

Nomogram to predict an endometrial thickness above 7.5 mm in the frozen embryo transfer cycle of women with a thin endometrium.

Reprod Biomed Online 2021 Nov 10. Epub 2021 Nov 10.

Reproductive Hospital Affiliated to Shandong University, Jinan Shandong 250001, China; Centre for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan Shandong 250012, China; National Research Centre for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan Shandong 250012, China.

Research Question: Which characteristics of patients with a thin endometrium (endometrial thickness [EMT] ≤7.5 mm on human chorionic gonadotrophin [HCG] trigger day) suggest the possibility of an EMT >7.5 mm in the subsequent frozen cycle?

Design: Data were collected from the university-affiliated Centre for Reproductive Medicine between January 2013 and September 2019. Read More

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November 2021

Maternal advanced age, single parenthood, and ART increase the risk of child morbidity up to five years of age.

BMC Pediatr 2022 Jan 14;22(1):39. Epub 2022 Jan 14.

Department of Obstetrics and Gynecology, and Department of Biomedical and Clinical Sciences, Linköping University, SE-581 85, Linköping, Sweden.

Background: Advanced maternal age, single status and use of assisted reproductive technology (ART) are increasing in mothers in high-income countries, and all are known risk factors for negative obstetric outcomes. Less is known about their long-term consequences for childhood morbidity. Thus, the aim of this study was to investigate morbidity up to five years of age, in the children of older, single, and/or ART-treated mothers. Read More

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January 2022

Ex vivo development of the entire mouse fetal reproductive tract by using microdissection and membrane-based organ culture techniques.

Authors:
Shuai Jia Fei Zhao

Differentiation 2022 Jan 6;123:42-49. Epub 2022 Jan 6.

Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, 53706, USA. Electronic address:

Ex vivo explant culture is an appealing alternative to in vivo studies on fetal reproductive organ development. There is extensive literature on ex vivo methods of growing the fetal gonad. However, a method for culturing the whole fetal reproductive tract that has a different shape and size has not been documented. Read More

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January 2022

Expression of tissue factor and TF-mediated integrin regulation in HTR-8/SVneo trophoblast cells.

J Reprod Immunol 2022 Jan 10;150:103473. Epub 2022 Jan 10.

Reproductive Immunology and Molecular Pathology (RIMP) Lab, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore, Tamil Nadu, India. Electronic address:

Placenta is a crucial source of Tissue Factor (TF) to initiate coagulation. As far as the TF is concern, aberrant expression of TF has been reported to have a significant role in thrombosis, inflammation, cancer metastasis and atherosclerosis. It is evident that TF and TF-FVIIa complex has major roles in the disease process beyond hemostasis and thrombosis. Read More

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January 2022

Cytoplasmic incompatibility in the semivoltine longicorn beetle Acalolepta fraudatrix (Coleoptera: Cerambycidae) double infected with Wolbachia.

PLoS One 2022 14;17(1):e0261928. Epub 2022 Jan 14.

Computational Bio Big-Data Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology, Shinjuku-ku, Tokyo, Japan.

Wolbachia are obligatory endosymbiotic α-proteobacteria found in many arthropods. They are maternally inherited, and can induce reproductive alterations in the hosts. Despite considerable recent progress in studies on the associations between Wolbachia and various taxonomic groups of insects, none of the researches have revealed the effects of Wolbachia on longicorn beetles as the host insect. Read More

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January 2022

Change in metabolic parameters and reproductive hormones from baseline to 6-month hormone therapy.

Medicine (Baltimore) 2022 Jan;101(1):e28361

Department of Gynecology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.

Abstract: Adequate evidence showed hormone therapy (HT) reduces the risk of new-onset diabetes in midlife women by decreasing fasting glucose and insulin. However, the improvement of these diabetic biomarkers varied with each individual in clinical observations. The objective of our study was to investigate potential baseline factors associated with the change of fasting glucose and insulin during HT. Read More

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January 2022

The central vein sign helps in differentiating multiple sclerosis from its mimickers: lessons from Fabry disease.

Eur Radiol 2022 Jan 14. Epub 2022 Jan 14.

Department of Advanced Biomedical Sciences, University "Federico II", Via Pansini, 5, 80131, Naples, Italy.

Objectives: Although the use of specific MRI criteria has significantly increased the diagnostic accuracy of multiple sclerosis (MS), reaching a correct neuroradiological diagnosis remains a challenging task, and therefore the search for new imaging biomarkers is crucial. This study aims to evaluate the incidence of one of the emerging neuroradiological signs highly suggestive of MS, the central vein sign (CVS), using data from Fabry disease (FD) patients as an index of microvascular disorder that could mimic MS.

Methods: In this retrospective study, after the application of inclusion and exclusion criteria, MRI scans of 36 FD patients and 73 relapsing-remitting (RR) MS patients were evaluated. Read More

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January 2022

A panel of three serum microRNA can be used as potential diagnostic biomarkers for nasopharyngeal carcinoma.

J Clin Lab Anal 2022 Jan 14:e24194. Epub 2022 Jan 14.

Department of Urology, Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Clinical College of Anhui Medical University, Shenzhen, China.

Background: Nasopharyngeal carcinoma is cancer with unique epidemiological characteristics, showing obvious ethnicity, gender, and geographical prevalence. More and more evidence shows that microRNAs are stable in serum and are specific to different tumor types. Therefore, miRNA is a new non-invasive biomarker for cancer detection. Read More

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January 2022

Maternal Perinatal Exposure to Dibutyl Phthalate Promotes Ovarian Dysfunction in Adult Female Offspring via Downregulation of TGF-β2 and TGF-β3.

Reprod Sci 2022 Jan 14. Epub 2022 Jan 14.

Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, People's Republic of China.

Maternal exposure to dibutyl phthalate (DBP) may result in ovarian dysfunction in female offspring. However, the underlying mechanisms remain elusive. Pregnant Sprague-Dawley rats were intraperitoneally injected with different doses of DBP, estradiol, and corn oil from gestational day 7 until the end of lactation. Read More

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January 2022

Optimization of Adipose Tissue Cryopreservation Techniques in a Murine Model.

Plast Reconstr Surg Glob Open 2021 Nov 11;9(11):e3926. Epub 2021 Nov 11.

Unidad de Genética, Hospital Universitario y Politécnico La Fe, Valencia, Spain.

Background: The aim of this study was to develop an adipose tissue (AT) cryopreservation protocol that is effective, simple, and maintains the functionality and viability of AT after thawing and transplantation.

Methods: Two cryopreservation temperatures (T°), -20°C and -80°C, and two cryoprotective agents (CPAs), trehalose and hydroxyethyl starch (HES), were compared first in an experimental study, using a slowfreezing protocol. The five experimental groups were the following: (a) Fresh AT (control group), (b) T = -20°C, 10%HES, (c) T = -80°C, 10%HES, (d) T = -20°C, 0. Read More

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November 2021

Contract Remedies Need Not Undercompensate Aspiring Parents When Cryopreserved Reproductive Material Is Lost or Destroyed: Recovery of Consequential Damages for Emotional Disturbance When Breach of Contract Results in the Lost Opportunity to Become Pregnant with One's Own Biological Child.

Authors:
Joseph M Hnylka

J Law Health 2021 ;35(1):99-145

The Center for Disease Control and Prevention (CDC) has reported that the use of assisted reproductive technology (ART) has doubled over the past decade. In vitro fertilization (IVF) is the most prevalent form of ART. During IVF, a woman's eggs are extracted, fertilized in a laboratory setting, and then implanted in the uterus. Read More

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January 2021

Optimal individualization of patient-oriented ovarian stimulation in Japanese assisted reproductive technology clinics, a review for unique setting with advanced-age patients.

J Obstet Gynaecol Res 2022 Jan 13. Epub 2022 Jan 13.

Department of Obstetrics and Gynecology, Saitama Medical University, Saitama, Japan.

Ovarian stimulation is a key issue in assisted reproductive technology (ART) treatment. ART practice in Japan is unique with various types of ovarian stimulation protocols, which may contribute to lower pregnancy rates compared to other countries. This review aims to clarify optimal individualized ovarian stimulation for improving the pregnancy rate per one oocyte retrieval cycle in Japan. Read More

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January 2022

Characterizing toxicity pathways of fluoxetine to predict adverse outcomes in adult fathead minnows (Pimephales promelas).

Sci Total Environ 2022 Jan 10:152747. Epub 2022 Jan 10.

Toxicology Centre, University of Saskatchewan, Saskatoon, SK S7N 5B3, Canada; School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK S7N 5C8, Canada. Electronic address:

Current ecotoxicity testing programs are impeded as they predominantly rely on slow and expensive animal tests measuring adverse outcomes. Therefore, new approach methodologies (NAMs) increasingly involve short-term mechanistic assays that employ molecular endpoints to predict adverse outcomes of regulatory relevance. This study aimed to elucidate the application of NAMs in adult fathead minnows using fluoxetine (FLX) as a model compound. Read More

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January 2022

Ovarian cancer and KiSS-1 gene expression: A consideration of the use of Kisspeptin plus Kisspeptin aptamers in diagnostics and therapy.

Eur J Pharmacol 2022 Jan 11;917:174752. Epub 2022 Jan 11.

Department of Pharmacy, School of Applied Sciences, University of Huddersfield, Huddersfield, HD1 3DH, United Kingdom. Electronic address:

Gynaecological cancers continue to present a significant health burden upon the health of the global female population. This deficit is most prominent with ovarian cancer which possesses the lowest survival rate compared to all other cancers occurring within this anatomical region, with an annual UK-mortality of 7,300. The poor tolerability and selectively of the treatment options that are currently available is likely to have contributed to this high mortality rate thus, demonstrating the need for the development of enhanced therapeutic approaches. Read More

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January 2022

Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1.

Autophagy 2022 Jan 13:1-15. Epub 2022 Jan 13.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

Ovarian granulosa cells (GCs) proliferate and differentiate along with follicular growth, and this is indispensable for oocyte development and female fertility. Although the role of macroautophagy/autophagy in ovarian function has been reported, its contribution to the regulation of GC characteristics remains elusive. The siRNA-mediated knockdown of two key autophagy-related genes and and the autophagy inhibitor chloroquine were used to interfere with autophagy in GCs. Read More

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January 2022

Individual, household, and community-level predictors of modern contraceptive use among married women in Cameroon: a multilevel analysis.

Int Health 2022 Jan 12. Epub 2022 Jan 12.

School of International Development and Global Studies, University of Ottawa, Ottawa, ON K1N 6N5, Canada.

Background: Unintended pregnancy remains a major public health and socio-economic problem in sub-Saharan African countries, including Cameroon. Modern contraceptive use can avert unintended pregnancy and its related problems. In Cameroon, the prevalence of modern contraceptive use is low. Read More

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January 2022

Genetic diversity and structure of , a plant species with extremely small populations.

Plant Divers 2021 Dec 8;43(6):472-479. Epub 2021 Jun 8.

Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming, 650224, Yunnan, China.

is a critically endangered species with important ornamental value and is also a plant species with extremely small populations. In this study, we used double digest restriction-site-associated DNA sequencing (ddRAD) technology to assess the genetic diversity, genetic structure and demographic history of the three extant populations of . Analysis of SNPs indicated that populations have a high genetic diversity (π = 0. Read More

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December 2021

Conceptualizing aged reproduction: genetic connectedness, son preference and assisted reproduction in North India.

Reprod Biomed Soc Online 2022 Mar 14;14:182-191. Epub 2021 Dec 14.

Department of Liberal Arts, Indian Institute of Technology Hyderabad, Sangareddy, Telangana, India.

In this paper, I explore the narratives on the administration of assisted reproductive technology (ART) treatments amongst ageing men and women who are past their 'reproductive prime'. I use the phrase 'past their reproductive prime' with caution as ART has the capability to resurrect the desire, quest and conception of children amongst these ageing women and men. In rural agrarian Haryana in North India, ART panders to, and provides, potent narratives for perpetuation of the patrilineage for genetic continuity through the male line. Read More

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The making of 'old eggs': the science of reproductive ageing between fertility and anti-ageing technologies.

Authors:
Nolwenn Bühler

Reprod Biomed Soc Online 2022 Mar 23;14:169-181. Epub 2021 Aug 23.

University of Neuchâtel, Switzerland.

This article proposes going back in the history of reproductive medicine to shed light on the role of assisted reproductive technology (ART) in the making of 'old eggs'. Focusing on two key technologies - egg donation and cytoplasmic transfer - both of which contributed significantly to the production of scientific knowledge about reproductive ageing, the article suggests that ART can be analysed as 'in-vivo models' playing a pivotal role in the shift from age as a demographic variable to ageing understood in biological terms. It will shed light on the role of ART in locating age in the eggs and producing a cellular understanding of fertility decline. Read More

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Enthusiasm, concern and ambivalence in the Belgian public's attitude towards in-vitro gametogenesis.

Reprod Biomed Soc Online 2022 Mar 25;14:156-168. Epub 2021 Nov 25.

Department of Philosophy and Moral Sciences, Ghent University, Ghent, Belgium.

Given the controversial nature of research into in-vitro gametogenesis (IVG), this study set out to investigate the current attitudes towards IVG in the general Belgian population in order to anticipate potential future barriers and misunderstandings. A questionnaire was developed and incorporated into a web-based online survey and sent out to Belgians aged ≥ 18 years in September 2018 until a representative sample (by age, gender and region) of 1000 participants was reached. Respondents expressed an overall positive attitude towards IVG and its possible future applications, with the exception of the use of IVG in postmenopausal women. Read More

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The ART clock: Temporal limits to assisted reproduction.

Authors:
Heather Jacobson

Reprod Biomed Soc Online 2022 Mar 25;14:144-155. Epub 2021 Nov 25.

Department of Sociology and Anthropology, The University of Texas at Arlington, Arlington, TX, USA.

Conceptualizations of the 'biological clock' in popular imaginary in the USA centre on the temporal limits of fertility, with assisted reproductive technology (ART) an increasingly proposed answer to these constraints (at least in the public imaginary). In this study, I analyse how surrogates in the USA understand their own bioavailability for others' reproductive needs in the commercial ART market vis-à-vis their own reproductive trajectories. Based on interview data with gestational surrogates, I propose a new concept of the 'ART clock' to capture how time shapes the experiences of reproductive workers in the US fertility clinic. Read More

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Household food insecurity and dietary diversity of women of reproductive age among smallholder farming households in northwest Bangladesh.

Ecol Food Nutr 2022 Jan 13:1-24. Epub 2022 Jan 13.

Agricultural Systems and Engineering, Department of Food, Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, Klong Luang, Thailand.

Despite Bangladesh's remarkable progress in agricultural production over the past few decades, household food and nutrition insecurity persist, especially in rural areas. The nutrition security and dietary diversity are even more critical for women of reproductive age among smallholders. This study examined household food insecurity and dietary diversity of women of reproductive age in the rural areas of northwest Bangladesh. Read More

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January 2022

Seven challenges in the multiscale modeling of multicellular tissues.

WIREs Mech Dis 2022 Jan 4;14(1):e1527. Epub 2021 May 4.

School of Mathematics and Statistics, University of Melbourne, Parkville, Victoria, Australia.

The growth and dynamics of multicellular tissues involve tightly regulated and coordinated morphogenetic cell behaviors, such as shape changes, movement, and division, which are governed by subcellular machinery and involve coupling through short- and long-range signals. A key challenge in the fields of developmental biology, tissue engineering and regenerative medicine is to understand how relationships between scales produce emergent tissue-scale behaviors. Recent advances in molecular biology, live-imaging and ex vivo techniques have revolutionized our ability to study these processes experimentally. Read More

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January 2022

Novel exon mutation in SYCE1 gene is associated with non-obstructive azoospermia.

J Cell Mol Med 2022 Jan 12. Epub 2022 Jan 12.

Reproductive Medicine Center, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Henan Provincial People's Hospital of Henan University, Zhengzhou, China.

Non-obstructive azoospermia (NOA) is a common cause of male infertility, and genetic problems, such as chromosomal abnormalities and gene mutations, are important causes of NOA. Our centre received a case of NOA, in which no mature sperm was found during microdissection testicular sperm extraction. A postoperative pathological examination revealed that testicular spermatogenesis was blocked. Read More

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January 2022

The function of Nucleoporin 37 on mouse oocyte maturation and preimplantation embryo development.

J Assist Reprod Genet 2022 Jan 13. Epub 2022 Jan 13.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.

Purpose: Nucleoporin 37 (NUP37) has been reported to activate the YAP-TEAD signaling, which is crucial for early embryo development. However, whether NUP37 is involved in oocyte meiosis and embryo development remains largely unknown. The study aimed to clarify the function of Nup37 in oocyte maturation and early embryo development, and to explore the mechanism. Read More

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January 2022

Human INHBB gene variant (c.1079T>C:p.Met360Thr) alters testis germ cell content, but does not impact fertility in mice.

Endocrinology 2022 Jan 12. Epub 2022 Jan 12.

Department of Physiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.

Testicular derived inhibin B (α/βB dimers) acts in an endocrine manner to suppress pituitary production of follicle stimulating hormone (FSH), by blocking the actions of activins (βA/B/βA/B dimers). Previously, we identified a homozygous genetic variant (c.1079T>C:p. Read More

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January 2022