479,304 results match your criteria reproducibility


Biobridge: An Outlook on Translational Bioinks for 3D Bioprinting.

Adv Sci (Weinh) 2021 Dec 3:e2103469. Epub 2021 Dec 3.

Institute for Macromolecular Chemistry, University of Freiburg, Freiburg, 79104, Germany.

3D-bioprinting (3DBP) possesses several elements necessary to overcome the deficiencies of conventional tissue engineering, such as defining tissue shape a priori, and serves as a bridge to clinical translation. This transformative potential of 3DBP hinges on the development of the next generation of bioinks that possess attributes for clinical use. Toward this end, in addition to physicochemical characteristics essential for printing, bioinks need to possess proregenerative attributes, while enabling printing of stable structures with a defined biological function that survives implantation and evolves in vivo into functional tissue. Read More

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December 2021

Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications.

Nat Methods 2021 Dec 3. Epub 2021 Dec 3.

Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA.

For quality, interpretation, reproducibility and sharing value, microscopy images should be accompanied by detailed descriptions of the conditions that were used to produce them. Micro-Meta App is an intuitive, highly interoperable, open-source software tool that was developed in the context of the 4D Nucleome (4DN) consortium and is designed to facilitate the extraction and collection of relevant microscopy metadata as specified by the recent 4DN-BINA-OME tiered-system of Microscopy Metadata specifications. In addition to substantially lowering the burden of quality assurance, the visual nature of Micro-Meta App makes it particularly suited for training purposes. Read More

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December 2021

Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma.

Thyroid Res 2021 Dec 3;14(1):27. Epub 2021 Dec 3.

Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

Background: Three-dimensional (3D) cell culture methods are identified for simulating the biological microenvironment and demonstrating more similarity to in vivo circumstances. Anaplastic thyroid carcinoma (ATC) is a lethal endocrine malignancy. Despite different treatment approaches, no improvement in the survival rate of the patients has been shown. Read More

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December 2021

Are Three-Dimensional-Printed Foot Orthoses Able to Cover the Podiatric Physician's Needs?

J Am Podiatr Med Assoc 2021 Sep;111(5)

Background: Current management of foot pain requires foot orthoses (FOs) with various design features (eg, wedging, height) and specific mechanical properties (eg, hardness, volume). Development of additive manufacturing (three-dimensional [3-D] printing) raises the question of applying its technology to FO manufacturing. Recent studies have demonstrated the physical benefits of FO parts with specific mechanical properties, but none have investigated the relationship between honeycomb architecture (HcA) infilling density and Shore A hardness of thermoplastic polyurethane (TPU) used to make FOs, which is the aim of this study. Read More

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September 2021

Automated long-term two-photon imaging in head-fixed walking Drosophila.

J Neurosci Methods 2021 Nov 30:109432. Epub 2021 Nov 30.

Center of Advanced European Studies and Research (caesar), Bonn, Germany. Electronic address:

Background: The brain of Drosophila shows dynamics at multiple timescales, from the millisecond range of fast voltage or calcium transients to functional and structural changes occurring over multiple days. To relate such dynamics to behavior requires monitoring neural circuits across these multiple timescales in behaving animals.

New Method: Here, we develop a technique for automated long-term two-photon imaging in fruit flies, during wakefulness and extended bouts of immobility, as typically observed during sleep, navigating in virtual reality over up to seven days. Read More

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November 2021

Tools and applications for integrative analysis of DNA methylation in social insects.

Mol Ecol Resour 2021 Dec 3. Epub 2021 Dec 3.

Departments of Biology and Computer Science, Indiana University, Bloomington, IN, 47405, USA.

DNA methylation is a common epigenetic signaling tool and an important biological process which is widely studied in a large array of species. The presence, level, and function of DNA methylation varies greatly across species. In some insects, DNA methylation systems are minimal, and overall methylation rates tend to be low in all studied insect species. Read More

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December 2021

Self-Terminated Electroless Deposition of Surfactant-Free and Monodispersed Pt Nanoparticles on Carbon Fiber Microelectrodes for Sensitive Detection of HO Released from Living Cells.

Anal Chem 2021 Dec 3. Epub 2021 Dec 3.

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, People's Republic of China.

We report a self-terminated electroless deposition method to prepare surfactant-free and monodispersed Pt nanoparticle (NP)-modified carbon fiber microelectrodes (Pt NP/CFEs) for electrochemical detection of hydrogen peroxide (HO) released from living cells. The surfactant-free and monodispersed Pt NPs with a uniform size of 65 nm are spontaneously deposited on a CFE surface by immersing an exposed carbon fiber (CF) of CFE in the PtCl solution, in which an exposed CF can be used as the reducing agent and stabilizer. A self-terminated electroless deposition method is demonstrated, in which the density and size of Pt NPs on a CFE surface do not increase when the reaction time increases from 20 to 60 min. Read More

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December 2021

Best Practices for Authentication of Cell Lines to Ensure Data Reproducibility and Integrity.

Radiat Res 2021 Dec 3. Epub 2021 Dec 3.

Division of Translational Radiation Sciences, Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, Maryland 21201.

Cell line misidentification and contamination are major contributors to the reproducibility crisis in academic research. Authentication of cell lines provides assurances of the data generated; however, commercially available cells are often not subjected to rigorous identification testing. In this study, commercially available cell lines underwent testing to confirm cell identity and purity. Read More

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December 2021

Dispersive liquid-liquid microextraction of 11-nor-Δ9-tetrahydrocannabinol-carboxylic acid applied to urine testing.

Bioanalysis 2022 Jan;14(2):87-100

Campinas Poison Control Center, Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, 13083-859, Brazil.

THC-COOH is the major metabolite of Δ-tetrahydrocannabinol commonly tested in urine to determine cannabis intake. In this study, a method based on dispersive liquid-liquid microextraction was developed for testing THC-COOH in urine. Hydrolyzed urine specimens were extracted via dispersive liquid-liquid microextraction with acetonitrile (disperser solvent) and chloroform (extraction solvent). Read More

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January 2022

Comet Assay for the Detection of Single and Double-Strand DNA Breaks.

Methods Mol Biol 2022 ;2422:263-269

Department of Pathology and Medicine & Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA.

DNA damage is a common event that occurs during the life span of the majority of cells, as a result of aberrant energy metabolism, exposure to radiation, DNA damaging chemicals, and viral infections. Rapid, sensitive, and economical methods for the detection of DNA damage offer valuable insights into DNA repair, drug genotoxicity, and biomonitoring. The comet assay, or single cell electrophoresis, has emerged as a popular method for detecting single- and double-stranded DNA breaks in single cells. Read More

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January 2022

Usability and reproducibility of three tools to assess medical students and residents in emergency medicine.

AEM Educ Train 2021 Aug 1;5(4):e10704. Epub 2021 Aug 1.

Emergency Department Hôpital Pitié-Salpêtrière Assistance Publique - Hôpitaux de Paris Paris France.

Objectives: It is critical to assess competency of medical students and residents in emergency medicine (EM) during undergraduate and graduate medical education. However, very few valid tools exist to assess both technical and nontechnical skills in the specific context of EM. Three Acute Care Assessment Tools (ACAT 1, 2, and 3) have been previously developed for three acute care conditions: cardiac arrest (1), coma (2), and acute respiratory failure (3). Read More

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Quantification of Duloxetine in the Bacterial Culture and Medium to Study Drug-gut Microbiome Interactions.

Bio Protoc 2021 Nov 5;11(21):e4214. Epub 2021 Nov 5.

European Molecular Biology Laboratory, Heidelberg, Germany.

Expanding our understanding of drug-gut bacteria interactions requires high-throughput drug measurements in complex bacterial cultures. Quantification of drugs in the cultures, media, and cell pellets is prone to strong matrix effects. We have developed a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method for quantifying duloxetine from high-throughput gut-drug interaction experiments. Read More

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November 2021

Multifunctional Gold Nano-Cytosensor With Quick Capture, Electrochemical Detection, and Non-Invasive Release of Circulating Tumor Cells for Early Cancer Treatment.

Front Bioeng Biotechnol 2021 11;9:783661. Epub 2021 Nov 11.

CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China.

Circulating tumor cells (CTCs) are metastatic tumor cells that shed into the blood from solid primary tumors, and their existence significantly increases the risk of metastasis and recurrence. The timely discovery and detection of CTCs are of considerable importance for the early diagnosis and treatment of metastasis. However, the low number of CTCs hinders their detection. Read More

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November 2021

Rapid Detection of by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay.

Front Cell Infect Microbiol 2021 10;11:762472. Epub 2021 Nov 10.

College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China.

Cysticercosis is a neglected tropical disease caused by the larvae of in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of . Read More

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November 2021

Investigation of methods to extract confocal function parameters for the depth resolved determination of attenuation coefficients using OCT in intralipid samples, titanium oxide phantoms, and in vivo human retinas.

Biomed Opt Express 2021 Nov 7;12(11):6814-6830. Epub 2021 Oct 7.

LaserLaB, Department of Physics and Astronomy, Vrije Universiteit, Amsterdam, The Netherlands.

The attenuation coefficient provides a quantitative parameter for tissue characterization and can be calculated from optical coherence tomography (OCT) data, but accurate determination requires compensation for the confocal function. We present extensive measurement series for extraction of the focal plane and the apparent Rayleigh length from the ratios of OCT images acquired with different focus depths and compare these results with two alternative approaches. By acquiring OCT images for a range of different focus depths the optimal focus plane difference is determined for intralipid and titanium oxide (TiO) phantoms with different scatterer concentrations, which allows for calculation of the attenuation coefficient corrected for the confocal function. Read More

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November 2021

Strengthening the clinical research workforce through a competency-based orientation program: Process outcomes and lessons learned across three academic health institutions.

J Clin Transl Sci 2021 13;5(1):e178. Epub 2021 Sep 13.

Department of Pediatrics, Penn State College of Medicine, Hershey, PA, USA.

Clinical research coordinators are increasingly tasked with a multitude of complex study activities critical to scientific rigor and participant safety, though more than half report not receiving appropriate training. To determine the reproducibility of an established clinical research workforce orientation program, collaborative partners across Clinical and Translational Science Award institutions seeded core principles and structure from Mayo Clinic's Clinical Research Orientation program within Penn State University and the University of Mississippi Medical Center from 2019 to 2021. Training concepts were established and tied to those domains deemed critical by the Joint Task Force for Clinical Trial Competency for the conduct of clinical research at the highest levels of safety and quality possible. Read More

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September 2021

The reliability of the AOSpine Thoracolumbar Spine Injury Classification System in children: an international validation study.

J Child Orthop 2021 Oct;15(5):472-478

Department of Orthopaedic Surgery, Boston Children's Hospital, Boston, Massachusetts, United States.

Purpose: To evaluate the AOSpine Thoracolumbar Spine Injury Classification System and if it is reliable and reproducible when applied to the paediatric population globally.

Methods: A total of 12 paediatric orthopaedic surgeons were asked to review MRI and CT imaging of 25 paediatric patients with thoracolumbar spine traumatic injuries, in order to determine the classification of the lesions observed. The evaluators classified injuries into primary categories: A, B and C. Read More

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October 2021

A Standardized Brain Molecular Atlas: A Resource for Systems Modeling and Simulation.

Front Mol Neurosci 2021 10;14:604559. Epub 2021 Nov 10.

Blue Brain Project, École Polytechnique Fédérale de Lausanne, Geneva, Switzerland.

Accurate molecular concentrations are essential for reliable analyses of biochemical networks and the creation of predictive models for molecular and systems biology, yet protein and metabolite concentrations used in such models are often poorly constrained or irreproducible. Challenges of using data from different sources include conflicts in nomenclature and units, as well as discrepancies in experimental procedures, data processing and implementation of the model. To obtain a consistent estimate of protein and metabolite levels, we integrated and normalized data from a large variety of sources to calculate Adjusted Molecular Concentrations. Read More

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November 2021

Ultrasensitive early detection of insulin antibody employing novel electrochemical nano-biosensor based on controllable electro-fabrication process.

Talanta 2022 Feb 14;238(Pt 1):122947. Epub 2021 Oct 14.

Nanomaterials and Polymer Nanocomposites Laboratory, School of Engineering, University of British Columbia, Kelowna, British Columbia, V1V 1V7, Canada. Electronic address:

An ultrasensitive novel electrochemical nano-biosensor for rapid detection of insulin antibodies against diabetes antigens was developed in this research. The presence of insulin antibodies has been demonstrated to be a strong predictor for the development of type 1 diabetes in individuals who do not have diabetes but are genetically predisposed. The proposed nano-biosensor fabrication process was based on the optimized sequential electropolymerization of polyaniline and electrodeposition of gold nanoparticles on the surface of the functionalized gold electrode. Read More

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February 2022

A novel ratiometric electrochemical sensor based on dual-monomer molecularly imprinted polymer and Pt/CoO for sensitive detection of chlorpromazine hydrochloride.

Anal Chim Acta 2022 Jan 3;1190:339245. Epub 2021 Nov 3.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, PR China.

In this work, a novel signal on/off ratiometric electrochemical sensor for the selective detection of chlorpromazine (CPZ) was developed. The sensor was constructed by electrodepositing dual-monomer molecularly imprinted polymer (DMMIP) film on the surface of Pt/CoO nanoparticles modified glassy carbon electrode, using CPZ as template molecule, methylene blue and catechol as functional monomers. The copolymerization of two monomers increased the diversity of functional groups for binding template molecules, and enhanced stability. Read More

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January 2022

Electrochemical detection of chloride ions using Ag-based electrodes obtained from compact disc.

Anal Chim Acta 2022 Jan 27;1190:339215. Epub 2021 Oct 27.

Dipartimento di Ingegneria, Università Degli Studi di Palermo, Italy. Electronic address:

In this work electrochemical sensors fabricated from compact disc material (waste or new) are used to quantify chloride ions in different types of samples. All three electrodes, working, counter, and pseudo-reference electrodes, were fabricated from the compact disc and directly used. Different parameters were studied in order to demonstrate the possibility of using this waste material for efficient and low-cost electrochemical sensors. Read More

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January 2022

Facile synthesis of neodymium stannate nanoparticles an effective electrocatalyst for the selective detection of dimetridazole in biological samples.

Anal Chim Acta 2022 Jan 1;1190:339234. Epub 2021 Nov 1.

Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei, 106, Taiwan, ROC.

In this work, pyrochlore neodymium stannate nanoparticles (NdSnO NP) have been synthesized by a facile co-precipitation technique and employed as an electrode material on GCE for the determination of dimetridazole (DM) drug. The physical properties and texture of the NdSnO NP were characterized by PXRD, Raman spectroscopy, FE-SEM, EDX mapping, XPS, and HR-TEM analytical studies. The electrocatalytic investigation of NdSnO NP/GCE was carried out by CV, and DPV techniques. Read More

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January 2022

Methodological quality and reporting standards in systematic reviews with meta-analysis of physical activity studies: a report from the Strengthening the Evidence in Exercise Sciences Initiative (SEES Initiative).

Syst Rev 2021 Dec 2;10(1):304. Epub 2021 Dec 2.

National Institute of Science and Technology for Health Technology Assessment (IATS/HCPA), Hospital de Clínicas de Porto Alegre, Clinical Research Center, Porto Alegre, RS, Brazil.

Background: Several resources have been developed (e.g., reporting guidelines) to promote high-standard practices in health research. Read More

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December 2021

Reproducibility of Morphologic Parameters of the International Endocervical Adenocarcinoma Criteria and Classification System and Correlation With Clinicopathologic Parameters: A Multi-Institutional Study.

Int J Gynecol Pathol 2021 Dec 3. Epub 2021 Dec 3.

Departments of Pathology (P.B., İ.K., N.K.) Gynecology and Obstetrics (Ç.T., D.V.), School of Medicine, Koc University Department of Pathology, School of Medicine, Acibadem University (E.H.Z.), Istanbul Departments of Pathology (N.H., Ö.Ö.) Gynecology and Obstetrics (A.A.), School of Medicine, Baskent University Department of Pathology, School of Medicine, Gazi University (Ö.E.), Ankara, Turkey.

Endocervical adenocarcinomas (ECAs) have been recently reclassified according to their morphologic features linked to etiology by the International Endocervical Adenocarcinoma Criteria and Classification (IECC) and this system is adopted by WHO 2020. This classification separates the ECAs as human papillomavirus (HPV)-associated (HPVA) and HPV-independent (HPVI) subtypes. According to WHO 2020, high risk (HR)-HPV association can be histologically recognized by the presence of luminal mitoses and apoptosis. Read More

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December 2021

Facile synthesis of petal-like VS anchored onto graphene nanosheets for the rapid sensing of toxic pesticide in polluted water.

Ecotoxicol Environ Saf 2021 Nov 30;228:113021. Epub 2021 Nov 30.

Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 100-715, Republic of Korea. Electronic address:

Fenitrothion (FT) is a toxic phosphorothioate insecticide that can easily contaminate aquatic environments, leading to a detrimental effect on the aquatic species and harmful endocrine disrupter effects on human health. Therefore, it is vital to develop a reliable methodology for the accurate and precise real-time sensing of carcinogenic FT in water samples at trace concentration to ensure environmental safety. We aim to fabricate the low-cost VS-attached reduced graphene oxide (RGO) sheets via a simple hydrothermal approach. Read More

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November 2021

Ultrasensitive detection of food colorant sunset yellow using nickel nanoparticles promoted lettuce-like spinel CoO anchored GO nanosheets.

Food Chem Toxicol 2021 Nov 29;159:112725. Epub 2021 Nov 29.

Department of Machinery Construction, Institute for Nanomaterials, Advanced Technologies and Innovation (CXI), Technical University of Liberec, Studentská 1402/2, 461 17 Liberec 1, Czech Republic.

Synthetic food colorants are extensively used across the globe regardless of the fact that they induce deleterious side effects when used in higher amounts. In this work, a novel electrochemical sensor based on nickel nanoparticles doped lettuce-like CoO anchored graphene oxide (GO) nanosheets was developed for effective detection of sulfonated azo dye sunset yellow widely used as a food colorant. Hydrothermal synthesis was adopted for the preparation of lettuce-like spinel CoO nanoparticles and Ni-CoO NPs/GO nanocomposite was prepared using ecofriendly and economical sonochemical method. Read More

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November 2021

A translational rat model for ex vivo lung perfusion of pre-injured lungs after brain death.

PLoS One 2021 2;16(12):e0260705. Epub 2021 Dec 2.

Department of Surgery, University Medical Center Groningen, Groningen, The Netherlands.

The process of brain death (BD) detrimentally affects donor lung quality. Ex vivo lung perfusion (EVLP) is a technique originally designed to evaluate marginal donor lungs. Nowadays, its potential as a treatment platform to repair damaged donor lungs is increasingly studied in experimental models. Read More

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December 2021

Statistical reproducibility for pairwise -tests in pharmaceutical research.

Stat Methods Med Res 2021 Dec 2:9622802211041765. Epub 2021 Dec 2.

Data Sciences & Quantitative Biology, Discovery Sciences, 468087R&D, AstraZeneca, Cambridge, UK.

This paper investigates statistical reproducibility of the -test. We formulate reproducibility as a predictive inference problem and apply the nonparametric predictive inference method. Within our research framework, statistical reproducibility provides inference on the probability that the same test outcome would be reached, if the test were repeated under identical conditions. Read More

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December 2021

Reproducibility and diurnal variation of the directional sensitivity of the cerebral pressure-flow relationship in men and women.

J Appl Physiol (1985) 2021 Dec 2. Epub 2021 Dec 2.

Department of Kinesiology, Faculty of Medicine, Université Laval, Québec, Canada.

The cerebral pressure-flow relationship has directional sensitivity, meaning the augmentation in cerebral blood flow is attenuated when mean arterial pressure (MAP) increases vs MAP decreases. We employed repeated squat-stands (RSS) to quantify it using a novel metric. However, its within-day reproducibility and the impacts of diurnal variation and biological sex are unknown. Read More

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December 2021

The within- and between-laboratories reproducibility and predictive capacity of Amino acid Derivative Reactivity Assay using 4 mM test chemical solution: Results of ring study implemented at five participating laboratories.

J Appl Toxicol 2021 Dec 1. Epub 2021 Dec 1.

Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Division of Pharmaceutical Sciences, Okayama University, Okayama, Japan.

Amino acid derivative reactivity assay (ADRA) for skin sensitization was adopted as an alternative method in the 2019 OECD Guideline for the Testing of Chemicals (OECD TG 442C). The molar ratio of the nucleophilic reagent to the test chemicals in the reaction solution was set to 1:50. Imamura et al. Read More

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December 2021