8,276 results match your criteria repress transcription


In situ approaches show the limitation of the spoilage potential of Juniperus phoenicea L. essential oil against cold-tolerant Pseudomonas fluorescens KM24.

Appl Microbiol Biotechnol 2021 May 14. Epub 2021 May 14.

Department of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, PL-60-624, Poznan, Poland.

The present study aimed to elucidate the effect of subinhibitory concentrations (sub-MICs) of juniper essential oil (EO), α-pinene, and sabinene on the quorum-sensing (QS)-mediated proteolytic and lipolytic properties of Pseudomonas fluorescens KM24. These activities were verified under in situ conditions, in which sub-MICs of the agents altered the morphology of KM24 cells. RNA-Seq studies revealed key coding sequences (CDSs)/genes related to QS and the proteolytic/lipolytic activities of pseudomonads. Read More

View Article and Full-Text PDF

RNA polymerase I subunit 12 plays opposite roles in cell proliferation and migration.

Biochem Biophys Res Commun 2021 May 10;560:112-118. Epub 2021 May 10.

College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, 430065, China. Electronic address:

RNA polymerase I (Pol I) is responsible for the synthesis of the majority of ribosomal RNA molecules in eukaryotes. Pol I subunit 12 (RPA12) is involved in the transcriptional termination and lipid metabolism in yeast. However, its role in human cells hasn't been investigated so far. Read More

View Article and Full-Text PDF

The miR166- SlHB15A Regulatory Module controls Ovule Development and Parthenocarpic Fruit Set under Adverse Temperatures in Tomato.

Mol Plant 2021 May 5. Epub 2021 May 5.

Institute of Plant Sciences Paris-Saclay, INRAE, CNRS, Université Paris-Saclay, Orsay 91405, France. Electronic address:

Fruit set is inhibited by adverse temperatures, with consequences on yield. We isolated a tomato mutant producing fruits under non-permissive hot temperatures and identified the causal gene as SlHB15A, belonging to class-III homeodomain leucine-zipper transcription factors (HD-ZipIII). SlHB15A loss-of-function mutants display aberrant ovule development that mimics transcriptional changes occurring in fertilized ovules and leads to parthenocarpic fruit set under optimal and non-permissive temperatures, in field and glasshouse conditions. Read More

View Article and Full-Text PDF

PAQR3 inhibits proliferation and aggravates ferroptosis in acute lymphoblastic leukemia through modulation Nrf2 stability.

Immun Inflamm Dis 2021 May 6. Epub 2021 May 6.

Department of Hematology, Yixing People's Hospital, Yixing City, Jiangsu Province, China.

Introduction: Acute lymphoblastic leukemia (ALL) is a usual hematological tumor, which was featured by malignant proliferation of lymphoid progenitor cells. Many important factors participate into the regulation of ALL, including proteins. PAQR3 (also named RKTG) has been proved to take part in many human cancers by acting as a tumor suppressor. Read More

View Article and Full-Text PDF

Activation of HERV-K(HML-2) disrupts cortical patterning and neuronal differentiation by increasing NTRK3.

Cell Stem Cell 2021 Apr 26. Epub 2021 Apr 26.

Institute of Virology, Helmholtz Zentrum München, Neuherberg, Germany; Developmental Biology and Center for Stem Cell Biology, Memorial Sloan Kettering Cancer Center, New York, NY, USA. Electronic address:

The biological function and disease association of human endogenous retroviruses (HERVs) are largely elusive. HERV-K(HML-2) has been associated with neurotoxicity, but there is no clear understanding of its role or mechanistic basis. We addressed the physiological functions of HERV-K(HML-2) in neuronal differentiation using CRISPR engineering to activate or repress its expression levels in a human-pluripotent-stem-cell-based system. Read More

View Article and Full-Text PDF

The Ghd7 Transcription Factor Represses the ARE1 Expression to Enhance Nitrogen Utilization and Grain Yield in Rice.

Mol Plant 2021 Apr 27. Epub 2021 Apr 27.

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Beijing 100101, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572025, China. Electronic address:

The genetic improvement of nitrogen use efficiency (NUE) of crops is vital for grain productivity and sustainable agriculture. However, the regulatory mechanism of NUE remains largely elusive. Here, we find that the rice Grain number, plant height, and heading date7 (Ghd7) gene genetically acts upstream of ABC1 REPRESSOR1 (ARE1), a negative regulator of NUE, to positively regulate nitrogen utilization. Read More

View Article and Full-Text PDF

NF-κB p65 regulates hepatic lipogenesis by promoting nuclear entry of ChREBP in response to a high carbohydrate diet.

J Biol Chem 2021 Apr 27:100714. Epub 2021 Apr 27.

School of Basic Sciences, Indian Institute of Technology Mandi, Mandi-175001, H.P, India,. Electronic address:

Overconsumption of sucrose and other sugars has been associated with non-alcoholic fatty liver disease (NAFLD). Reports suggest that hepatic de novo lipogenesis (DNL) is an important contributor to and regulator of carbohydrate-induced hepatic lipid accumulation in NAFLD. The mechanisms responsible for the increase in hepatic DNL due to overconsumption of carbohydrate diet are less than clear; however, literatures suggest high carbohydrate diet to activate the lipogenic transcription factor carbohydrate response element-binding protein (ChREBP), which further transcribes genes involved in DNL. Read More

View Article and Full-Text PDF

RNA Interference: Story and Mechanisms.

Authors:
Mouldy Sioud

Methods Mol Biol 2021 ;2282:1-15

Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital-Radiumhospitalet, Oslo, Norway.

The discovery that gene expression can be silenced by exogenously introduced double-stranded RNAs into cells unveiled a hidden level of gene regulation by a variety of small RNA pathways, which are involved in regulating endogenous gene expression, defending against virus infections, and protecting the genome from invading transposons, both at the posttranscriptional and epigenetic levels. All endogenous RNA interference pathways share a conserved effector complex, which contains at least an argonaute protein and a short single-stranded RNA. Such argonaute-RNA complexes can repress the transcription of genes, target mRNA for site-specific cleavage, or block mRNA translation into proteins. Read More

View Article and Full-Text PDF
January 2021

Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems.

Science 2021 04;372(6541)

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

CRISPR-Cas systems provide RNA-guided adaptive immunity in prokaryotes. We report that the multisubunit CRISPR effector Cascade transcriptionally regulates a toxin-antitoxin RNA pair, CreTA. CreT (Cascade-repressed toxin) is a bacteriostatic RNA that sequesters the rare arginine tRNA (transfer RNA with anticodon UCU). Read More

View Article and Full-Text PDF

DNA Methylation of the Angiotensinogen Gene, , and the Aldosterone Synthase Gene, in Cardiovascular Diseases.

Int J Mol Sci 2021 Apr 27;22(9). Epub 2021 Apr 27.

Department of Endocrinology and Metabolism, Kanazawa University Hospital, Kanazawa 920-8641, Japan.

Angiotensinogen (AGT) and aldosterone play key roles in the regulation of blood pressure and are implicated in the pathogenesis of cardiovascular diseases. DNA methylation typically acts to repress gene transcription. The aldosterone synthase gene is regulated by angiotensin II and potassium. Read More

View Article and Full-Text PDF

The EAR Motif in the MADS Transcription Factor AGAMOUS-Like 15 Is Not Necessary to Promote Somatic Embryogenesis.

Plants (Basel) 2021 Apr 13;10(4). Epub 2021 Apr 13.

Department of Plant and Soil Sciences, University of Kentucky, Plant Science Building, Lexington, KY 40502, USA.

AGAMOUS-like 15 (AGL15) is a member of the MADS domain family of transcription factors (TFs) that can directly induce and repress target gene expression, and for which promotion of somatic embryogenesis (SE) is positively correlated with accumulation. An ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif of form LxLxL within the carboxyl-terminal domain of AGL15 was shown to be involved in repression of gene expression. Here, we examine whether AGL15's ability to repress gene expression is needed to promote SE. Read More

View Article and Full-Text PDF

Atypical E2Fs either Counteract or Cooperate with RB during Tumorigenesis Depending on Tissue Context.

Cancers (Basel) 2021 Apr 23;13(9). Epub 2021 Apr 23.

Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CT Utrecht, The Netherlands.

E2F-transcription factors activate many genes involved in cell cycle progression, DNA repair, and apoptosis. Hence, E2F-dependent transcription must be tightly regulated to prevent tumorigenesis, and therefore metazoan cells possess multiple E2F regulation mechanisms. The best-known is the Retinoblastoma protein (RB), which is mutated in many cancers. Read More

View Article and Full-Text PDF

Immune Transcriptome of Cells Infected with Enterovirus Strains Obtained from Cases of Autoimmune Thyroid Disease.

Microorganisms 2021 Apr 19;9(4). Epub 2021 Apr 19.

Global Virus Network, University of Insubria, 21100 Varese, Italy.

Background: Hashimoto's thyroiditis and Graves' disease are autoimmune thyroid disorders (AITD) of unknown origin. Enterovirus (EV) infection of thyroid cells has been implicated as a possible initiator of cell damage and of organ-specific autoimmunity. We asked whether persistent infection of human epithelial cells with EV strains obtained from thyroid tissue of AITD patients could be associated with transcriptional changes capable of fostering immunopathology. Read More

View Article and Full-Text PDF

An insulator blocks access to enhancers by an illegitimate promoter, preventing repression by transcriptional interference.

PLoS Genet 2021 Apr 26;17(4):e1009536. Epub 2021 Apr 26.

Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America.

Several distinct activities and functions have been described for chromatin insulators, which separate genes along chromosomes into functional units. Here, we describe a novel mechanism of functional separation whereby an insulator prevents gene repression. When the homie insulator is deleted from the end of a Drosophila even skipped (eve) locus, a flanking P-element promoter is activated in a partial eve pattern, causing expression driven by enhancers in the 3' region to be repressed. Read More

View Article and Full-Text PDF

Reciprocal Regulation between Fur and Two RyhB Homologs in , and Roles of RyhBs in Biofilm Formation.

Biomed Environ Sci 2021 Apr;34(4):299-308

Wuxi School of Medicine, Jiangnan University, Wuxi 214122, Jiangsu, China.

Objective: To investigate reciprocal regulation between Fur and two RyhB homologs in ( ), as well as the roles of RyhBs in biofilm formation.

Methods: Regulatory relationships were assessed by a combination of colony morphology assay, primer extension, electrophoretic mobility shift assay and DNase I footprinting.

Results: Fur bound to the promoter-proximal DNA regions of and to repress their transcription, while both RyhB1 and RyhB2 repressed the expression of Fur at the post-transcriptional level. Read More

View Article and Full-Text PDF

Characterization of DinJ-YafQ toxin-antitoxin module in Tetragenococcus halophilus: activity, interplay, and evolution.

Appl Microbiol Biotechnol 2021 May 20;105(9):3659-3672. Epub 2021 Apr 20.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.

Tetragenococcus halophilus is a moderately halophilic lactic acid bacterium widely used in high-salt food fermentation because of its coping ability under various stress conditions. Bacterial toxin-antitoxin (TA) modules are widely distributed and play important roles in stress response, but those specific for genus Tetragenococcus have never been explored. Here, a bona fide TA module named DinJ-YafQ was characterized in T. Read More

View Article and Full-Text PDF

Cultivation techniques to study lanthanide metal interactions in the haloalkaliphilic Type I methanotroph "Methylotuvimicrobium buryatense" 5GB1C.

Methods Enzymol 2021 10;650:237-259. Epub 2021 Mar 10.

Department of Chemical Engineering, University of Washington, Seattle, WA, United States; Department of Microbiology, University of Washington, Seattle, WA, United States.

Lanthanide metals are commonly used in technological devices including batteries, computers, catalysts and magnets. Despite their important properties, mining difficulties and pollution concerns limit the number of mines worldwide. Because of these concerns, biometallurgy is an attractive possibility for lanthanide extraction from recycled materials or from contaminated sites. Read More

View Article and Full-Text PDF

ZNF410 represses fetal globin by singular control of CHD4.

Nat Genet 2021 May 15;53(5):719-728. Epub 2021 Apr 15.

Division of Hematology/Oncology, Boston Children's Hospital, Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Stem Cell Institute, Broad Institute, Department of Pediatrics, Harvard Medical School, Boston, MA, USA.

Known fetal hemoglobin (HbF) silencers have potential on-target liabilities for rational β-hemoglobinopathy therapeutic inhibition. Here, through transcription factor (TF) CRISPR screening, we identify zinc-finger protein (ZNF) 410 as an HbF repressor. ZNF410 does not bind directly to the genes encoding γ-globins, but rather its chromatin occupancy is concentrated solely at CHD4, encoding the NuRD nucleosome remodeler, which is itself required for HbF repression. Read More

View Article and Full-Text PDF

Deconvoluting global cytokine signaling networks in natural killer cells.

Nat Immunol 2021 May 15;22(5):627-638. Epub 2021 Apr 15.

Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Cytokine signaling via signal transducer and activator of transcription (STAT) proteins is crucial for optimal antiviral responses of natural killer (NK) cells. However, the pleiotropic effects of both cytokine and STAT signaling preclude the ability to precisely attribute molecular changes to specific cytokine-STAT modules. Here, we employed a multi-omics approach to deconstruct and rebuild the complex interaction of multiple cytokine signaling pathways in NK cells. Read More

View Article and Full-Text PDF

PU.1 enforces quiescence and limits hematopoietic stem cell expansion during inflammatory stress.

J Exp Med 2021 Jun;218(6)

Division of Hematology, University of Colorado Anschutz Medical Campus, Aurora, CO.

Hematopoietic stem cells (HSCs) are capable of entering the cell cycle to replenish the blood system in response to inflammatory cues; however, excessive proliferation in response to chronic inflammation can lead to either HSC attrition or expansion. The mechanism(s) that limit HSC proliferation and expansion triggered by inflammatory signals are poorly defined. Here, we show that long-term HSCs (HSCLT) rapidly repress protein synthesis and cell cycle genes following treatment with the proinflammatory cytokine interleukin (IL)-1. Read More

View Article and Full-Text PDF

Nuclear ErbB2 represses DEPTOR transcription to inhibit autophagy in breast cancer cells.

Cell Death Dis 2021 Apr 14;12(4):397. Epub 2021 Apr 14.

Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

ErbB2, a classical receptor tyrosine kinase, is frequently overexpressed in breast cancer cells. Although the role of ErbB2 in the transmission of extracellular signals to intracellular matrix has been widely studied, the functions of nuclear ErbB2 remain largely elusive. Here, we report a novel function of nuclear ErbB2 in repressing the transcription of DEPTOR, a direct inhibitor of mTOR. Read More

View Article and Full-Text PDF

CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica.

Methods Mol Biol 2021 ;2307:95-109

Chemical and Environmental Engineering, University of California Riverside, Riverside, CA, USA.

Recent developments in RNA-guided nuclease technologies have advanced the engineering of a wide range of organisms, including the nonconventional yeast Yarrowia lipolytica. Y. lipolytica has been the focus of a range of synthetic biology and metabolic engineering studies due to its high capacity to synthesize and accumulate intracellular lipids. Read More

View Article and Full-Text PDF
January 2021

A class of independently evolved transcriptional repressors in plant RNA viruses facilitates viral infection and vector feeding.

Proc Natl Acad Sci U S A 2021 Mar;118(11)

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China;

Plant viruses employ diverse virulence strategies to achieve successful infection, but there are few known general strategies of viral pathogenicity and transmission used by widely different plant viruses. Here, we report a class of independently evolved virulence factors in different plant RNA viruses which possess active transcriptional repressor activity. Rice viruses in the genera , , and all have transcriptional repressors that interact in plants with the key components of jasmonic acid (JA) signaling, namely mediator subunit OsMED25, OsJAZ proteins, and OsMYC transcription factors. Read More

View Article and Full-Text PDF

A single cell view of the transcriptome during lateral root initiation in Arabidopsis thaliana.

Plant Cell 2021 Apr 3. Epub 2021 Apr 3.

Department of Biology.

Root architecture is a major determinant of plant fitness and is under constant modification in response to favorable and unfavorable environmental stimuli. Beyond impacts on the primary root, the environment can alter the position, spacing, density, and length of secondary or lateral roots. Lateral root development is among the best-studied examples of plant organogenesis, yet there are still many unanswered questions about its earliest steps. Read More

View Article and Full-Text PDF

A fungal sirtuin modulates development and virulence in the insect pathogen, Beauveria bassiana.

Environ Microbiol 2021 Apr 5. Epub 2021 Apr 5.

Department of Microbiology and Cell Science, University of Florida, Bldg. 981, Museum Rd, Gainesville, FL, 32611, USA.

Chromatin transitions are mediated in part by acetylation/deacetylation post-translational modifications of histones. Histone deacetylases, e.g. Read More

View Article and Full-Text PDF

The function of non-coding RNAs in idiopathic pulmonary fibrosis.

Open Med (Wars) 2021 26;16(1):481-490. Epub 2021 Mar 26.

Department of Cardiovascular Diseases, First Affiliated Hospital of Baotou Medical College, Baotou, Inner Mongolia, China.

Non-coding ribonucleic acids (ncRNAs) are a diverse group of RNA molecules that are mostly not translated into proteins after transcription, including long non-coding RNAs (lncRNAs) with longer than 200 nucleotides non-coding transcripts and microRNAs (miRNAs) which are only 18-22 nucleotides. As families of evolutionarily conserved ncRNAs, lncRNAs activate and repress genes via a variety of mechanisms at both transcriptional and translational levels, whereas miRNAs regulate protein-coding gene expression mainly through mRNA silencing. ncRNAs are widely involved in biological functions, such as proliferation, differentiation, migration, angiogenesis, and apoptosis. Read More

View Article and Full-Text PDF

WNT responsive SUMOylation of ZIC5 promotes murine neural crest cell development via multiple effects on transcription.

J Cell Sci 2021 Mar 26. Epub 2021 Mar 26.

Early Mammalian Development Laboratory, John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia

Zinc finger of the cerebellum (Zic) proteins act as classical transcription factors to promote transcription of the gene during neural crest cell specification. Additionally, they can act as co-factors that bind TCF molecules to repress WNT/β-catenin-dependent transcription without contacting DNA. Here, we show ZIC activity at the neural plate border is influenced by WNT-dependent SUMOylation. Read More

View Article and Full-Text PDF

CENTRORADIALIS maintains shoot meristem indeterminacy by antagonizing THORNIDENTITY1 in Citrus.

Curr Biol 2021 Mar 18. Epub 2021 Mar 18.

Department of Molecular, Cellular and Developmental Biology, Yale University, 260 Whitney Avenue, New Haven, CT 06520, USA; Department of Ecology and Evolutionary Biology, Yale University, 165 Prospect Street, New Haven, CT 06520, USA. Electronic address:

Differential regulation of stem cell activity in shoot meristems contributes to the wide variation in shoot architecture. In most Citrus species, a thorn meristem and a dormant axillary meristem co-localize at each leaf base, offset from each other in a spiral phyllotactic pattern. We recently identified THORNIDENTITY1 (TI1) and THORNIDENTITY2 (TI2), encoding TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors, as necessary for the termination of meristem proliferation and concomitant thorn production in Citrus. Read More

View Article and Full-Text PDF

GARP transcription factors repress Arabidopsis nitrogen starvation response via ROS-dependent and -independent pathways.

J Exp Bot 2021 May;72(10):3881-3901

BPMP, Univ Montpellier, CNRS, INRA, SupAgro, Montpellier, France.

Plants need to cope with strong variations of nitrogen availability in the soil. Although many molecular players are being discovered concerning how plants perceive NO3- provision, it is less clear how plants recognize a lack of nitrogen. Following nitrogen removal, plants activate their nitrogen starvation response (NSR), which is characterized by the activation of very high-affinity nitrate transport systems (NRT2. Read More

View Article and Full-Text PDF