5,262 results match your criteria represents intermediate


Microstructural changes of the wall of uterine horn in the domestic cat (Felis silvestris catus) during postnatal development.

Theriogenology 2021 May 3;170:23-35. Epub 2021 May 3.

Department of Histology and Embryology, Faculty of Veterinary Medicine and Animal Science, Poznan University of Life Sciences, Wojska Polskiego 71C, PL-60-625, Poznań, Poland.

The study describes the details of histological changes occurring during the postnatal development of the wall of the uterine horn in the European shorthair domestic cat. Light microscope observations and morphometric analysis were carried out to investigate the rate of development of the wall of the uterine horns in order to estimate the maturing time. Uterine horns of newborn and 1week-old cats are fetal-like and showing smooth, nonfolded endometrium lined with pseudostratified epithelium, deprived of uterine glands, and poorly developed two-layered myometrium. Read More

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Moderate Hypofractionated Radiotherapy for Localized Prostate Cancer: The Triumph of Radiobiology.

Rev Recent Clin Trials 2021 May 8. Epub 2021 May 8.

Radiotherapy Unit, 2nd Department of Radiology, ATTIKON University Hospital, National & Kapodistrian University of Athens, Medical School, Athens, Greece.

Background: Radiotherapy represents one of the main therapeutic modalities for localized prostate cancer. In the last two decades, emerging data regarding the radiobiology of prostate cancer suggests a very low α/β value which has led the scientific community to evaluate the potential advantage of hypofractionation.

Objective: The aim of this manuscript is to present the rationale of prostate radiobiology and the medical evidence of moderate hypofractionation for prostate cancer. Read More

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Cryo-Focused Ion Beam Lamella Preparation Protocol for in Situ Structural Biology.

Methods Mol Biol 2021 ;2305:301-322

CEITEC, Masaryk University, Brno, Czech Republic.

The advances in electron cryo-microscopy have enabled high-resolution structural studies of vitrified macromolecular complexes in situ by cryo-electron tomography (cryo-ET). Since utilization of cryo-ET is generally limited to the specimens with thickness < 500 nm, a complex sample preparation protocol to study larger samples such as single eukaryotic cells by cryo-ET was developed and optimized over the last decade. The workflow is based on the preparation of a thin cellular lamella by cryo-focused ion beam milling (cryo-FIBM) from the vitrified cells. Read More

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January 2021

Case Report-Staged brachytherapy achieving complete metabolic response in unresectable oligometastatic colorectal cancer to the liver.

Oxf Med Case Reports 2021 Apr 28;2021(4):omab016. Epub 2021 Apr 28.

Department of Radiation Oncology, National University Cancer Institute Singapore.

Liver is the most common site for metastasis from colorectal cancer (CRC). Non-surgical treatment options for oligometastatic CRC confined to the liver which represents an intermediate state in the metastatic cascade are fast expanding. Currently, several liver-directed local therapeutic options are available, such as hepatic arterial infusion (HAI) therapy, radio-frequency ablation (RFA), transarterial chemoembolization (TACE), stereotactic body radiotherapy and high dose rate brachytherapy (HDRBT). Read More

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Light-Driven Enzymatic Decarboxylation of Dicarboxylic Acids.

ChemistryOpen 2021 May;10(5):553-559

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, West 7th Avenue, Tianjin, 300308, P. R. China.

Photodecarboxylase from Chlorella variabillis (CvFAP) is one of the three known light-activated enzymes that catalyzes the decarboxylation of fatty acids into the corresponding C1-shortened alkanes. Although the substrate scope of CvFAP has been altered by protein engineering and decoy molecules, it is still limited to mono-fatty acids. Our studies demonstrate for the first time that long chain dicarboxylic acids can be converted by CvFAP. Read More

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New Sub-Phenotyping of Subjects at High Risk of Type 2 Diabetes: What Are the Potential Clinical Implications?

Diabetes Ther 2021 May 4. Epub 2021 May 4.

Diabetes Centre, Second Department of Internal Medicine, Democritus University of Thrace, University Hospital of Alexandroupolis, Alexandroupolis, Greece.

Prediabetes is defined as a condition of abnormal glucose metabolism, characterised by plasma glucose above normal range but not as high as required for the diagnosis of diabetes mellitus (DM). It represents a heterogeneous entity of intermediate glucose metabolism, including impaired fasting glucose, impaired glucose tolerance, and borderline glycated haemoglobin. Prediabetes is being increasingly recognised as an important metabolic state not only predisposing to a higher probability of future progression to DM, but also to an increased risk of different micro- and macrovascular complications. Read More

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Homocysteine restrains hippocampal neurogenesis in focal ischemic rat brain by inhibiting DNA methylation.

Neurochem Int 2021 May 1;147:105065. Epub 2021 May 1.

Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin, 300070, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Center for International Collaborative Research on Environment, Nutrition and Public Health, Tianjin Medical University, Tianjin, 300070, China. Electronic address:

Ischemic stroke represents a major cause of mortality worldwide. An elevated level of homocysteine (Hcy) is recognized as a powerful risk factor of ischemic stroke. We previously reported that Hcy induces cytotoxicity and proliferation inhibition in neural stem cells (NSCs) derived from the neonatal rat hippocampus in vitro. Read More

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Evolving origin-of-transfer sequences on staphylococcal conjugative and mobilizable plasmids-who's mimicking whom?

Nucleic Acids Res 2021 May 3. Epub 2021 May 3.

Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, Australia.

In Staphylococcus aureus, most multiresistance plasmids lack conjugation or mobilization genes for horizontal transfer. However, most are mobilizable due to carriage of origin-of-transfer (oriT) sequences mimicking those of conjugative plasmids related to pWBG749. pWBG749-family plasmids have diverged to carry five distinct oriT subtypes and non-conjugative plasmids have been identified that contain mimics of each. Read More

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Development and Validation of a Simplified Score to Predict Early Relapse in Newly Diagnosed Multiple Myeloma (S-ERMM) in a Pooled Dataset of 2190 Patients.

Clin Cancer Res 2021 Apr 29. Epub 2021 Apr 29.

Myeloma Unit, Division of Hematology, University of Torino, Azienda Ospedaliero-Universitaria Città della Salute e della Scienza di Torino

Background: Despite the improvement of therapeutic regimens, several multiple myeloma (MM) patients still experience early relapse (ER). This subset of patients currently represents an unmet medical need.

Methods: We pooled data from 7 European multicenter phase II/III clinical trials enrolling 2190 newly diagnosed (ND)MM patients from 2003 to 2017. Read More

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Interpolation with Specified Error of a Point Series Belonging to a Monotone Curve.

Entropy (Basel) 2021 Apr 21;23(5). Epub 2021 Apr 21.

Faculty of Telecommunications, Computer Science and Electrical Engineering, UTP University of Science and Technology in Bydgoszcz, 85-796 Bydgoszcz, Poland.

The paper addresses the problem of modeling a smooth contour interpolating a point series belonging to a curve containing no special points, which represents the original curve with specified accuracy. The contour is formed within the area of possible location of the parts of the interpolated curve along which the curvature values are monotonously increased or decreased. The absolute interpolation error of the point series is estimated by the width of the area of possible location of the curve. Read More

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Utilization of Cumbeba () Residue: Drying Kinetics and Effect of Process Conditions on Antioxidant Bioactive Compounds.

Foods 2021 Apr 6;10(4). Epub 2021 Apr 6.

Department of Technology in Agroindustry, Federal Institute of Alagoas, Piranhas 57460-000, Brazil.

The residue generated from the processing of (cumbeba) fruit pulp represents a large amount of material that is discarded without proper application. Despite that, it is a raw material that is source of ascorbic acid, carotenoids and phenolic compounds, which are valued in nutraceutical diets for allegedly combating free radicals generated in metabolism. This research paper presents a study focused on the mathematical modeling of drying kinetics and the effect of the process on the level of bioactive of cumbeba residue. Read More

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Structure and Dynamics of an Archeal Monoglyceride Lipase from as Revealed by Crystallography and In Silico Analysis.

Biomolecules 2021 Apr 3;11(4). Epub 2021 Apr 3.

Laboratoire de Chimie Biologique Structurale, Department of Chemistry, Namur Medicine & Drug Innovation Center (NAMEDIC-NARILIS), Namur Research Institute for Life Sciences (NARILIS), University of Namur, Rue de Bruxelles 61, B-5000 Namur, Belgium.

The crystallographic analysis of a lipase from (PFL) previously annotated as a lysophospholipase revealed high structural conservation with other monoglyceride lipases, in particular in the lid domain and substrate binding pockets. In agreement with this observation, PFL was shown to be active on various monoacylglycerols. Molecular Dynamics (MD) studies performed in the absence and in the presence of ligands further allowed characterization of the dynamics of this system and led to a systematic closure of the lid compared to the crystal structure. Read More

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Redox-neutral S-nitrosation Mediated by a Dicopper Center.

Angew Chem Int Ed Engl 2021 Apr 29. Epub 2021 Apr 29.

Ohio State University, Department of Chemistry and Biochemistry, 100 W. 18th Ave, 43210, Columbus, UNITED STATES.

An redox-neutral S -nitrosation of thiol has been achieved at dicopper(I,I) center. Treatment of dicopper (I,I) complex with excess NO • and thiol generates a dicopper (I,I) di- S -nitrosothiol complex [Cu I Cu I (RSNO) 2 ] 2+ or dicopper (I,I) mono- S -nitrosothiol complex [Cu I Cu I (RSNO)] 2+ , which readily release RSNO in 88-94% yield. The S -nitrosation reaction proceeds through a mixed-valence [Cu II Cu III ( μ -O)( μ -NO)] 2+ species, which deprotonates RS-H at the basic μ -O site and nitrosates the RS - at the μ -NO site. Read More

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Synergistic Pd(0)/Rh(II) Dual Catalytic [6 + 3] Dipolar Cycloaddition for the Synthesis of Monocyclic Nine-Membered -Heterocycles and Their Alder-ene Rearrangement to Fused Bicyclic Compounds.

Org Lett 2021 May 29;23(9):3735-3740. Epub 2021 Apr 29.

Department of Chemistry and Nanoscience, Ewha Womans University, 03760 Seoul, Korea.

The catalytic construction of a monocyclic medium-sized -heterocyclic ring represents a formidable challenge in organic synthesis. Herein we report the synergistic palladium(0)/rhodium(II) dual catalytic cycloaddition of vinylpropylene carbonates with -sulfonyl-1,2,3-triazoles to afford monocyclic nine-membered -heterocycles. The catalytically generated 1,6-dipole-equivalent zwitterionic π-allyl palladium(II) complex and the 1,3-dipole-equivalent α-imino rhodium(II) carbenoid intermediate react with each other in a formal [6 + 3] dipolar cycloaddition to furnish nine-membered oxazonines, which can be transformed into -fused [4. Read More

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MeSCoT: The tool for quantitative trait simulation through the mechanistic modelling of genes' regulatory interactions.

G3 (Bethesda) 2021 Apr 26. Epub 2021 Apr 26.

Center for Quantitative Genetics and Genomics, Aarhus University, Tjele, Denmark.

This work represents a novel mechanistic approach to simulate and study genomic networks with accompanying regulatory interactions and complex mechanisms of quantitative trait formation. The approach implemented in MeSCoT software is conceptually based on the omnigenic genetic model of quantitative (complex) trait, and closely imitates the basic in vivo mechanisms of quantitative trait realization. The software provides a framework to study molecular mechanisms of gene-by-gene and gene-by-environment interactions underlying quantitative trait's realization and allows detailed mechanistic studies of impact of genetic and phenotypic variance on gene regulation. Read More

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BF·OEt-Catalyzed Unexpected Stereoselective Formation of 2,4--Diallyl-2-methyl-6-aryltetrahydro-2-pyrans with Quaternary Stereocenters.

J Org Chem 2021 May 27;86(9):6518-6527. Epub 2021 Apr 27.

Department of Organic Synthesis and Process Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad 500007, India.

The present manuscript describes a convenient, mild, and highly stereoselective method for the allylation of δ-hydroxy-α,β-unsaturated ketones having a benzylic hydroxyl group at the δ-position using allyltrimethylsilane mediated by BF·OEt, leading to 2,4-diallyl-2-methyl-6-aryltetrahydro-2-pyran ring systems with quaternary carbon stereogenic centers. This represents the first example of a tandem isomerization followed by one C-O and two C-C bond-forming reactions in one pot. The isolation of TMS-protected lactol as an intermediate from the reaction strongly supports the proposed mechanistic pathway. Read More

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Ecological Barrier Deterioration Driven by Human Activities Poses Fatal Threats to Public Health due to Emerging Infectious Diseases.

Engineering (Beijing) 2021 Jan 5. Epub 2021 Jan 5.

School of Environment, Tsinghua University, Beijing 100084, China.

The recent outbreak of coronavirus disease 2019 (COVID-19) and concerns about several other pandemics in the 21st century have attracted extensive global attention. These emerging infectious diseases threaten global public health and raise urgent studies on unraveling the underlying mechanisms of their transmission from animals to humans. Although numerous works have intensively discussed the cross-species and endemic barriers to the occurrence and spread of emerging infectious diseases, both types of barriers play synergistic roles in wildlife habitats. Read More

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January 2021

A Nazarov-Ene Tandem Reaction for the Stereoselective Construction of Spiro Compounds.

Chemistry 2021 Apr 25. Epub 2021 Apr 25.

Leibniz University Hanover: Leibniz Universitat Hannover, Institut für Organische Chemie, GERMANY.

The different reactivity of trienones under Lewis and Br ø nsted acids catalysis was investigated, resulting in distinct cyclization products and carbon backbones that originated either from a conjugate Prins cyclization or an interrupted Nazarov cyclization. In particular, an unprecedented Nazarov cyclization tandem reaction is presented, terminating the oxyallyl cation by an ene-type reaction, and leading stereoselectively to bicyclic spiro compounds. The terminal olefin of this motif represents a useful handle for further functionalization, making it a strategic intermediate in total syntheses. Read More

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Safety and Efficacy of Degradable Starch Microspheres Transcatheter Arterial Chemoembolization (DSM-TACE) in the Downstaging of Intermediate-Stage Hepatocellular Carcinoma (HCC) in Patients With a Child-Pugh Score of 8-9.

Front Pharmacol 2021 8;12:634087. Epub 2021 Apr 8.

Radiology Unit, Department of Experimental and Clinical Medicine, Magna Graecia University, Catanzaro, Italy.

According to the EASL Guidelines for the management of hepatocellular carcinoma, transcatheter arterial chemoembolization is the first-line treatment recommended for intermediate-stage HCC. Furthermore, it is widely accepted that patients beyond the Milan criteria can be considered for a liver transplant after successful downstaging to within the Milan criteria. Response to downstaging treatments significantly influences not just drop-outs, but also the rate of post-transplantation tumor recurrences. Read More

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In-planta production of the biodegradable polyester precursor 2-pyrone-4,6-dicarboxylic acid (PDC): Stacking reduced biomass recalcitrance with value-added co-product.

Metab Eng 2021 Apr 22;66:148-156. Epub 2021 Apr 22.

Joint BioEnergy Institute, EmeryStation East, 5885 Hollis St, 4th Floor, Emeryville, CA, 94608, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA. Electronic address:

2-Pyrone-4,6-dicarboxylic acid (PDC), a chemically stable intermediate that naturally occurs during microbial degradation of lignin by bacteria, represents a promising building block for diverse biomaterials and polyesters such as biodegradable plastics. The lack of a chemical synthesis method has hindered large-scale utilization of PDC and metabolic engineering approaches for its biosynthesis have recently emerged. In this study, we demonstrate a strategy for the production of PDC via manipulation of the shikimate pathway using plants as green factories. Read More

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Cerebrospinal Fluid Neurofilament Light Chain (NfL) Predicts Disease Aggressiveness in Amyotrophic Lateral Sclerosis: An Application of the D50 Disease Progression Model.

Front Neurosci 2021 6;15:651651. Epub 2021 Apr 6.

Hans Berger Department of Neurology, Jena University Hospital, Jena, Germany.

Amyotrophic lateral sclerosis (ALS) is a relentlessly progressive neurodegenerative disorder. As previous therapeutic trials in ALS have been severely hampered by patients' heterogeneity, the identification of biomarkers that reliably reflect disease progression represents a priority in ALS research. Here, we used the D50 disease progression model to investigate correlations between cerebrospinal fluid (CSF) neurofilament light chain (NfL) levels and disease aggressiveness. Read More

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Effect of silver nanoparticles on vancomycin resistant Staphylococcus aureus infection in critically ill patients.

Pathog Glob Health 2021 Apr 19:1-10. Epub 2021 Apr 19.

Anaesthesia and Intensive Care Department, Faculty of Medicine, Assiut University, Assiut, Egypt.

A prevalent increase in antimicrobial resistance represents a universal obstacle for the treatment of infection, especially in critically ill patients. Silver nanoparticles are defined as broad spectrum bactericidal agents, which might be effective against vancomycin resistant (VRSA). In this study, we examined the bactericidal efficacy of silver nanoparticles on VRSA in 150 blood and sputum samples isolated from intensive care patients. Read More

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Enterovirus-A71 exploits peripherin and Rac1 to invade the central nervous system.

EMBO Rep 2021 Apr 19:e51777. Epub 2021 Apr 19.

Infectious Diseases Translational Research Programme, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Enterovirus-A71 (EV-A71) has been associated with severe neurological forms of hand, foot, and mouth disease (HFMD). EV-A71 infects motor neurons at neuromuscular junctions (NMJs) to invade the central nervous system (CNS). Here, we investigate the role of peripherin (PRPH) during EV-A71 infection, a type III intermediate neurofilament involved in neurodegenerative conditions. Read More

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Influence of the Rear Interface on Composition and Photoluminescence Yield of CZTSSe Absorbers: A Case for an AlO Intermediate Layer.

ACS Appl Mater Interfaces 2021 Apr 18;13(16):19487-19496. Epub 2021 Apr 18.

Laboratory for Thin Films and Photovoltaics, Empa-Swiss Federal Laboratories for Materials Science and Technology, Ueberlandstrasse 129, Dübendorf 8600, Switzerland.

The rear interface of kesterite absorbers with Mo back contact represents one of the possible sources of nonradiative voltage losses (Δ) because of the reported decomposition reactions, an uncontrolled growth of MoSe, or a nonoptimal electrical contact with high recombination. Several intermediate layers (IL), such as MoO, TiN, and ZnO, have been tested to mitigate these issues, and efficiency improvements have been reported. However, the introduction of IL also triggers other effects such as changes in alkali diffusion, altered morphology, and modifications in the absorber composition, all factors that can also influence Δ. Read More

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Decoding of Attentional State Using High-Frequency Local Field Potential Is As Accurate As Using Spikes.

Cereb Cortex 2021 Apr 19. Epub 2021 Apr 19.

Centre for Neuroscience, Indian Institute of Science, Bangalore 560012, India.

Local field potentials (LFPs) in visual cortex are reliably modulated when the subject's focus of attention is cued into versus out of the receptive field of the recorded sites, similar to modulation of spikes. However, human psychophysics studies have used an additional attention condition, neutral cueing, for decades. The effect of neutral cueing on spikes was examined recently and found to be intermediate between cued and uncued conditions. Read More

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Nursing student attitudes toward dying patient care: A European multicenter cross-sectional study.

Acta Biomed 2021 03 31;92(S2):e2021018. Epub 2021 Mar 31.

Department of Medical Science, Udine University.

Background and aim of the work Nursing education plays a key role in preparing future nurses to deal with dying patients, which represents one of the most emotionally involving aspect of nursing. The aims of the study were to explore nursing students' attitudes towards care of dying patients in three different European contexts and to analyze the variables that can influence them. Methods We conducted an international multicenter cross-sectional study. Read More

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"Two Ships in a Bottle" Design for Zn-Ag-O Catalyst Enabling Selective and Long-Lasting CO Electroreduction.

J Am Chem Soc 2021 May 14;143(18):6855-6864. Epub 2021 Apr 14.

Department of Chemical Engineering, Waterloo Institute for Nanotechnology, Waterloo Institute for Sustainable Energy, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

Electrochemical CO reduction (CORR) using renewable energy sources represents a sustainable means of producing carbon-neutral fuels. Unfortunately, low energy efficiency, poor product selectivity, and rapid deactivation are among the most intractable challenges of CORR electrocatalysts. Here, we strategically propose a "two ships in a bottle" design for ternary Zn-Ag-O catalysts, where ZnO and Ag phases are twinned to constitute an individual ultrafine nanoparticle impregnated inside nanopores of an ultrahigh-surface-area carbon matrix. Read More

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PEQULIARITIES OF MORPHOGENESYS AND TOPOGRAPHY OF INFRAHYOID TRIANGLES IN HUMAN PREFETUSES AND FETUSES.

Wiad Lek 2021 ;74(1):102-106

MEDICAL BIOLOGY DEPARTMENT OF THE I. HORBACHEVSKY STATE MEDICAL UNIVERSITY, TERNOPIL, UKRAINE.

Objective: The aim: To investigate morphology and developmental features of anatomical structures in the infrahyoid triangles of human neck during prefetal and fetal periods of human ontogenesis.

Patients And Methods: Materials and methods: We have studied 30 specimens of human prefetuses from 7th till 12th week (16,0-82,0 mm of parieto-coccygeal length (PCL)) and 30 human fetuses aged from 4th till 10th month (84,0-360,0 mm PCL) of intrauterine development by the means of macro-, microscopy, morphometry, three-dimensional remodeling and statistical analyses.

Results: Results: We can observe anterior triangle in human fetuses after the time when common precursor muscular mass splits into two: the anterior and posterior portions which will give rise to the sternocleidomastoid and trapezoid muscles accordingly. Read More

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Modified PCR protocol to increase sensitivity for determination of bacterial community composition.

Microbiome 2021 04 13;9(1):90. Epub 2021 Apr 13.

Section of Critical Care, Department of Pediatrics, University of Colorado School of Medicine and Children's Hospital Colorado, 13123 E. 16th Ave. Box B395, Aurora, CO, 80045, USA.

Background: The objective of this project was to increase the sensitivity of sequence-based bacterial community determination without impacting community composition or interfering with cluster formation during sequencing. Two PCR protocols (standard and modified) were examined in airway samples where we observed a large range in bacterial load (3.1-6. Read More

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Association of Prehospital Oxygen Saturation to Inspired Oxygen Ratio With 1-, 2-, and 7-Day Mortality.

JAMA Netw Open 2021 Apr 1;4(4):e215700. Epub 2021 Apr 1.

Data Analysis Unit, Health Research Institute, Hospital de la Princesa, Madrid, Spain.

Importance: The early identification of patients at high risk of clinical deterioration represents one of the greatest challenges for emergency medical services (EMS).

Objective: To assess whether use of the ratio of prehospital oxygen saturation measured by pulse oximetry (Spo2) to fraction of inspired oxygen (Fio2) measured during initial contact by EMS with the patient (ie, the first Spo2 to Fio2 ratio) and 5 minutes before the patient's arrival at the hospital (ie, the second Spo2 to Fio2 ratio) can predict the risk of early in-hospital deterioration.

Design, Setting, And Participants: A prospective, derivation-validation prognostic cohort study of 3606 adults with acute diseases referred to 5 tertiary care hospitals in Spain was conducted between October 26, 2018, and June 30, 2020. Read More

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