38,755 results match your criteria reporter mouse


Targeting androgen-signaling in ILC2s protects from IL-33-driven lung inflammation, independently of KLRG1.

J Allergy Clin Immunol 2021 May 5. Epub 2021 May 5.

Background: Allergic asthma is more severe and frequent in women compared to men. In male mice, androgens negatively control group 2 innate lymphoid cells (ILC2) development and function by yet unknown mechanisms.

Objectives: Here, we investigated the impact of androgen on ILC2 homeostasis and IL-33-mediated inflammation in female lungs. Read More

View Article and Full-Text PDF

EHD3 positively regulated by NR5A1 participates in testosterone synthesis via endocytosis.

Life Sci 2021 May 5:119570. Epub 2021 May 5.

NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Pharmacy, Fudan University, Shanghai 200032, China; NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai 200032, China. Electronic address:

Aims: Increasing evidence has shown that hormone secretion is regulated by endocytosis. Eps15 homology domain-containing protein 3 (EHD3) is an endocytic-trafficking regulatory protein, but whether EHD3 is associated with testosterone secretion is not clear. This work aims to explore the role of EHD3 in testosterone synthesis. Read More

View Article and Full-Text PDF

Selective sweep for an enhancer involucrin allele identifies skin barrier adaptation out of Africa.

Nat Commun 2021 May 7;12(1):2557. Epub 2021 May 7.

Division of Dermatology, Department of Medicine, Washington University in St. Louis School of Medicine, St. Louis, MO, 63110, USA.

The genetic modules that contribute to human evolution are poorly understood. Here we investigate positive selection in the Epidermal Differentiation Complex locus for skin barrier adaptation in diverse HapMap human populations (CEU, JPT/CHB, and YRI). Using Composite of Multiple Signals and iSAFE, we identify selective sweeps for LCE1A-SMCP and involucrin (IVL) haplotypes associated with human migration out-of-Africa, reaching near fixation in European populations. Read More

View Article and Full-Text PDF

CircRNA_0026344 via exosomal miR-21 regulation of Smad7 is involved in aberrant cross-talk of epithelium-fibroblasts during cigarette smoke-induced pulmonary fibrosis.

Toxicol Lett 2021 May 4. Epub 2021 May 4.

School of Public Health, Southwest Medical University, Luzhou, 646000, Sichuan, People's Republic of China. Electronic address:

For smoking-induced pulmonary fibrosis (PF), a serious disease endangering human health, there is no effective clinical treatment. Aberrant epithelium-fibroblast cross-talk is involved in formation of the excessive extracellular matrix (ECM) that contributes to PF. Circular RNAs have been associated with various pulmonary diseases. Read More

View Article and Full-Text PDF

Harmine targets inhibitor of DNA binding-2 and activator protein-1 to promote preosteoclast PDGF-BB production.

J Cell Mol Med 2021 May 7. Epub 2021 May 7.

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, China.

Osteoporosis is one of the most common metabolic bone diseases affecting millions of people. We previously found that harmine prevents bone loss in ovariectomized mice via increasing preosteoclast platelet-derived growth factor-BB (PDGF-BB) production and type H vessel formation. However, the molecular mechanisms by which harmine promotes preosteoclast PDGF-BB generation are still unclear. Read More

View Article and Full-Text PDF

Retinoic acid induces NELFA-mediated 2C-like state of mouse embryonic stem cells associates with epigenetic modifications and metabolic processes in chemically defined media.

Cell Prolif 2021 May 7:e13049. Epub 2021 May 7.

The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, China.

Objectives: Mouse embryonic stem cells (ESCs) are derived from the inner cell mass of blastocyst-stage embryos and cultured in different culture media with varied pluripotency. Sporadically, a small population of ESCs exhibit 2-cell stage embryonic features in serum containing medium. However, whether ESCs can transit into 2-cell embryo-like (2C-like) cells in the chemically defined media remains largely unknown. Read More

View Article and Full-Text PDF

Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models.

Front Genet 2021 20;12:615491. Epub 2021 Apr 20.

CHU Nantes, Inserm, Centre de Recherche en Transplantation et Immunologie, UMR 1064, ITUN, Université de Nantes, Nantes, France.

The rat has been extensively used as a small animal model. Many genetically engineered rat models have emerged in the last two decades, and the advent of gene-specific nucleases has accelerated their generation in recent years. This review covers the techniques and advances used to generate genetically engineered rat lines and their application to the development of rat models more broadly, such as conditional knockouts and reporter gene strains. Read More

View Article and Full-Text PDF

MiR-195-5p Ameliorates Cerebral Ischemia-Reperfusion Injury by Regulating the PTEN-AKT Signaling Pathway.

Neuropsychiatr Dis Treat 2021 29;17:1231-1242. Epub 2021 Apr 29.

Department of Emergency, Tianjin First Central Hospital, Tianjin, 300192, People's Republic of China.

Background: MiR-195-5p has been shown to play crucial roles in tumor inhibition, but its biological functions in cerebral ischemia-reperfusion (I/R) injury are unclear.

Methods: To mimic cerebral I/R injury, mice were induced by transient middle cerebral artery occlusion (MCAO). Human brain microvascular endothelial cells (HBMVECs) were treated with oxygen-glucose deprivation (OGD) to mimic I/R injury in vitro. Read More

View Article and Full-Text PDF

Neuron replating - a powerful and versatile approach to study early aspects of neuron differentiation.

eNeuro 2021 May 6. Epub 2021 May 6.

Molecular Neurobiology Group, Institute of Physiological Chemistry, University of Marburg, 35032 Marburg, Germany

Neuron differentiation includes formation and outgrowth of neurites that differentiate into axons or dendrites. Directed neurite outgrowth is controlled by growth cones that protrude and retract actin-rich structures to sense environmental cues. These cues control local actin filament dynamics, steer growth cones towards attractants and away from repellents and navigate neurites through the developing brain. Read More

View Article and Full-Text PDF

miR-125a-5p in astrocytes attenuates peripheral neuropathy in type 2 diabetic mice through targeting TRAF6.

Endocrinol Diabetes Nutr 2021 May 3. Epub 2021 May 3.

Department of Neurology, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan City, Hubei Province 430081, China. Electronic address:

Introduction: Elimination or blocking of astrocytes could ameliorate neuropathic pain in animal models. MiR-125a-5p, expressed in astrocyte derived extracellular vesicles, could mediate astrocyte function to regulate neuron communication. However, the role of miR-125a-5p in DPN (diabetic peripheral neuropathy) remains elusive. Read More

View Article and Full-Text PDF

circ_NRIP1 is oncogenic in malignant development of esophageal squamous cell carcinoma (ESCC) via miR-595/SEMA4D axis and PI3K/AKT pathway.

Cancer Cell Int 2021 May 6;21(1):250. Epub 2021 May 6.

Department of Oncology, The Second Affiliated Hospital of Henan University of Chinese Medicine, No.6 Dongfeng Road, Jinshui District, Zhengzhou, 450002, Henan, China.

Background: The hsa_circ_0004771 derived from NRIP1 (called circ_NRIP1) is a recently identified oncogenic circRNA. Here, we intended to investigate the role and mechanism of circ_NRIP1 in esophageal squamous cell carcinoma (ESCC), a prevalent and aggressive type of esophageal cancer.

Methods: Expression of circ_NRIP1, miRNA-595-5p (miR-595) and semaphorin 4D (SEMA4D) was detected by RT-qPCR and western blotting. Read More

View Article and Full-Text PDF

Glyphosate exposure attenuates testosterone synthesis via NR1D1 inhibition of StAR expression in mouse Leydig cells.

Sci Total Environ 2021 Apr 25;785:147323. Epub 2021 Apr 25.

Northwest A&F University, Yangling 712100, Shaanxi, China; Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address:

Glyphosate is a broad-spectrum herbicide that impairs testosterone synthesis in mammals. Leydig cells (LCs), the primary producers of testosterone, demonstrate rhythmic expression of circadian clock genes both in vivo and in vitro. The nuclear receptor NR1D1 is an important clock component that constitutes the subsidiary transcriptional/translational loop in the circadian clock system. Read More

View Article and Full-Text PDF

MiR-205-5p suppresses angiogenesis in gastric cancer by downregulating the expression of VEGFA and FGF1.

Exp Cell Res 2021 May 3:112579. Epub 2021 May 3.

Department of General Surgery, Peking University First Hospital, Beijing 100034, China. Electronic address:

Anti-angiogenic therapy represents one of the most promising treatment modalities for human cancers. However, the response to antiangiogenic therapy in gastric cancer (GC) remains dismal. To help identify new strategies for antiangiogenic therapy in GC, we evaluated miR-205-5p expression in GC tissues from TCGA database and our hospital, and its functions in angiogenesis were explored in vitro and in vivo. Read More

View Article and Full-Text PDF

MiR-216a-5p ameliorates learning-memory deficits and neuroinflammatory response of Alzheimer's disease mice via regulation of HMGB1/NF-κB signaling.

Authors:
Peng Shao

Brain Res 2021 May 3:147511. Epub 2021 May 3.

Department of Rehabilitation Medicine, Yantaishan Hospital, Yantai 264001, China. Electronic address:

Objective: The objective of this study was to explore whether miR-216a-5p could affect the learning-memory ability and inflammatory response of Alzheimer's disease (AD) mice via regulation of the HMGB1/NF-κB pathway.

Methods: Mice were divided into the normal (wild-type C57BL/6 mice), AD (APP/PS1 double-transgenic mice), AD + miR-216a-5p, and AD + vector groups. The Morris water maze test was used to examine learning and memory ability. Read More

View Article and Full-Text PDF

Endogenous p53 expression in human and mouse is not regulated by its 3'UTR.

Elife 2021 May 6;10. Epub 2021 May 6.

Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, United States.

The gene encodes the tumor suppressor p53 which is functionally inactivated in many human cancers. Numerous studies suggested that 3'UTR-mediated p53 expression regulation plays a role in tumorigenesis and could be exploited for therapeutic purposes. However, these studies did not investigate post-transcriptional regulation of the native gene. Read More

View Article and Full-Text PDF

Downregulation of miR-497-5p Improves Sepsis-Induced Acute Lung Injury by Targeting IL2RB.

Biomed Res Int 2021 12;2021:6624702. Epub 2021 Apr 12.

Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Zhejiang Chinese Medical University, 318 Chaowang Road, Hangzhou, Zhejiang 310005, China.

Introduction: Acute lung injury (ALI) induced by sepsis is a process related to inflammatory reactions, which involves lung cell apoptosis and production of inflammatory cytokine. Here, lipopolysaccharide (LPS) was applied to stimulate the mouse or human normal lung epithelial cell line (BEAS-2B) to construct a sepsis model and , and we also investigated the effect of miR-497-5p on sepsis-induced ALI. . Read More

View Article and Full-Text PDF

Exercise alleviates cardiac remodelling in diabetic cardiomyopathy via the miR-486a-5p-Mst1 pathway.

Iran J Basic Med Sci 2021 Feb;24(2):150-159

Department of Cardiology, Xijing Hospital, Air Force Medical University of PLA, Xi'an 710032, P.R. China.

Objectives: Physical exercise has emerged as an effective therapy to mitigate cardiac remodelling in diabetic cardiomyopathy (DCM). The results of our previous studies revealed mammalian sterile 20-like kinase 1 (Mst1) is a key regulator of the progression of DCM. However, the precise molecular mechanism of physical exercise-induced cardiac protection and its association with Mst1 inhibition remain unclear. Read More

View Article and Full-Text PDF
February 2021

Reversal of Chemotherapy Resistance to Cisplatin in NSCLC by miRNA-195-5p via Targeting the Gene.

Pharmgenomics Pers Med 2021 28;14:497-508. Epub 2021 Apr 28.

Department of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy of Tianjin City, Tianjin, 300060, People's Republic of China.

Objective: To explore the mechanism of miR-195-5p in the pathogenesis non-small cell lung cancer (NSCLC) and cisplatin resistance.

Methods: The function of miR-195-5p in NSCLC and cisplatin resistance were determined by MTT, scratch assay, transwell assay, and nude mice xenograft experiments. miR-195-5p target gene was identified by dual-luciferase reporter assays and real-time PCR analysis. Read More

View Article and Full-Text PDF

IL-33 is produced by colon fibroblasts and differentially regulated in acute and chronic murine colitis.

Sci Rep 2021 May 5;11(1):9575. Epub 2021 May 5.

Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave, MLC 2010, Cincinnati, OH, 45229, USA.

IL-33 is upregulated in ulcerative colitis and has a protective role in chemically-induced acute murine colitis. We aimed to determine whether IL-33 influences Il10 chronic colitis and its cellular source in health and during colitis. Il10Il33 and Il10Il33 littermates developed colitis of similar severity. Read More

View Article and Full-Text PDF

Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter.

Sci Adv 2021 May 5;7(19). Epub 2021 May 5.

Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

Photoactivated genetically encoded voltage indicators (GEVIs) have the potential to enable optically sectioned voltage imaging at the intersection of a photoactivation beam and an imaging beam. We developed a pooled high-throughput screen to identify archaerhodopsin mutants with enhanced photoactivation. After screening ~10 cells, we identified a novel GEVI, NovArch, whose one-photon near-infrared fluorescence is reversibly enhanced by weak one-photon blue or two-photon near-infrared excitation. Read More

View Article and Full-Text PDF

Targeting a novel LncRNA SNHG15/miR-451/c-Myc signaling cascade is effective to hamper the pathogenesis of breast cancer (BC) in vitro and in vivo.

Cancer Cell Int 2021 Mar 31;21(1):186. Epub 2021 Mar 31.

Department of Breast and Thyroid Surgery, The 3rd Affiliated Teaching Hospital of Xinjiang Medical University (Affiliated Cancer Hospital), Suzhou East Street No. 789, Xinshi District, Urumqi, 830011, Xinjiang, China.

Background: To our knowledge, LncRNA SNHG15 exerted its tumor-promoting effects to facilitate the development of breast cancer (BC), but there still needed more data to elucidate the potential underlying mechanisms.

Methods: We examined genes expression status by performing Real-Time qPCR and Western Blot analysis, and cellular functions, including cell proliferation, viability, apoptosis, mobility, were measured by using the CCK-8 assay, colony formation assay, trypan blue staining assay, flow cytometer (FCM), transwell assay and wound scratch assay, respectively. The predicted targeting sites in LncRNA SNHG15, miR-451 and c-Myc 3'UTR were validated by dual-luciferase reporter gene system assay. Read More

View Article and Full-Text PDF

Peptide-coated polyurethane material reduces wound infection and inflammation.

Acta Biomater 2021 May 2. Epub 2021 May 2.

Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, SE, 22184 Lund, Sweden. Electronic address:

There is an urgent need for treatments that not only reduce bacterial infection that occurs during wounding but that also target the accompanying excessive inflammatory response. TCP-25, a thrombin-derived antibacterial peptide, scavenges toll-like receptor agonists such as endotoxins and lipoteichoic acid and prevents toll-like receptor-4 dimerization to reduce infection-related inflammation in vivo. Using a combination of biophysical, cellular, and microbiological assays followed by experimental studies in mouse and pig models, we show that TCP-25, when delivered from a polyurethane (PU) material, exerts anti-infective and anti-inflammatory effects in vitro and in vivo. Read More

View Article and Full-Text PDF

Immunohistochemical and Genetic Labeling of Hairy and Glabrous Skin Innervation.

Curr Protoc 2021 May;1(5):e121

Vollum Institute, Oregon Health & Science University, Portland, Oregon.

Cutaneous innervation is an essential component of the mammalian sensory nervous system. During development, genetically and morphologically diverse subtypes of sensory neurons use distinct molecular pathways to innervate end organs or form free nerve endings in glabrous and hairy skin. Peripheral neurons can be damaged by acute injury or degenerate due to chronic conditions including diabetes and chemotherapy, leading to peripheral neuropathy. Read More

View Article and Full-Text PDF

The use of transgenics in the laboratory axolotl.

Dev Dyn 2021 May 5. Epub 2021 May 5.

Mount Desert Island Biological Laboratory (MDIBL), Salisbury, USA.

The ability to generate transgenic animals sparked a wave of research committed to implementing such technology in a wide variety of model organisms. Building a solid base of ubiquitous and tissue-specific reporter lines has set the stage for later interrogations of individual cells or genetic elements. Compared to other widely used model organisms such as mice, zebrafish and fruit flies, there are only a few transgenic lines available in the laboratory axolotl (Ambystoma mexicanum), although their number is steadily expanding. Read More

View Article and Full-Text PDF

EZH2 is involved in psoriasis progression by impairing miR-125a-5p inhibition of SFMBT1 and leading to inhibition of the TGFβ/SMAD pathway.

Ther Adv Chronic Dis 2021 15;12:2040622320987348. Epub 2021 Apr 15.

Department of Dermatology, The Second Hospital of Jilin University, No. 218, Ziqiang Road, Changchun, Jilin Province 130041, P.R. China.

Aims: In this study, we aimed to decipher the impact of enhancer of zeste homolog 2 (EZH2) in psoriasis as well as the underlying mechanism.

Methods: A mouse model of psoriasis was developed by means of imiquimod induction, with the expression of EZH2, microRNA-125a-5p (miR-125a-5p), and SFMBT1 determined. The role of EZH2, miR-125a-5p, and SFMBT1 in malignant phenotypes of HaCaT cells and the development of psoriasis was subsequently investigated through gain- and loss-of-function experiments. Read More

View Article and Full-Text PDF

The effects of conditioning and lentiviral vector pseudotype on short- and long-term airway reporter gene expression in mice.

Hum Gene Ther 2021 May 5. Epub 2021 May 5.

Women's and Children's Hospital Adelaide, 6053, Respiratory and sleep medicine, North Adelaide, South Australia, Australia;

A gene-addition therapy into the conducting airway epithelium is a potential cure for cystic fibrosis lung disease. Achieving sustained lung gene expression has proven difficult due to the natural barriers of the lung. The development of lentiviral (LV) vectors pseudotyped with viral envelopes that have a natural tropism to the airway has enabled persistent gene expression to be achieved in vivo. Read More

View Article and Full-Text PDF

An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation.

Cells 2021 Apr 30;10(5). Epub 2021 Apr 30.

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Fluorescent reporter mouse lines and Cre/Flp recombinase driver lines play essential roles in investigating various molecular functions . Now that applications of the CRISPR/Cas9 genome-editing system to mouse fertilized eggs have drastically accelerated these knock-in mouse generations, the next need is to establish easier, quicker, and cheaper methods for knock-in donor preparation. Here, we reverify and optimize the phospho-PCR method to obtain highly pure long single-stranded DNAs (ssDNAs) suitable for knock-in mouse generation via genome editing. Read More

View Article and Full-Text PDF

CCL17-expressing dendritic cells in the intestine are preferentially infected by Salmonella but CCL17 plays a redundant role in systemic dissemination.

Immun Inflamm Dis 2021 May 4. Epub 2021 May 4.

Immunology and Environment, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany.

Introduction: Salmonella spp. are a recognized and global cause of serious health issues from gastroenteritis to invasive disease. The mouse model of human typhoid fever, which uses Salmonella enterica serovar Typhimurium (STM) in susceptible mouse strains, has revealed that the bacteria gain access to extraintestinal tissues from the gastrointestinal tract to cause severe systemic disease. Read More

View Article and Full-Text PDF

An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin.

PLoS Negl Trop Dis 2021 May 4;15(5):e0009058. Epub 2021 May 4.

Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

Talaromyces marneffei is a thermally dimorphic fungus that causes opportunistic systemic mycoses in patients with AIDS or other immunodeficiency syndromes. The purpose of this study was to develop an immunochromatographic strip test (ICT) based on a solid phase sandwich format immunoassay for the detection of T. marneffei antigens in clinical urine specimens. Read More

View Article and Full-Text PDF

Circ_0017956 promotes the proliferation and metastasis of non-small cell lung cancer through regulating miR-515-5p/ITGB8 axis.

Authors:
Na Li Dan Zhu

Cell Cycle 2021 May 4:1-13. Epub 2021 May 4.

Department of Respiratory, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.

Circular RNAs (circRNAs) have been reported to exert vital roles in the tumorigenesis of non-small cell lung cancer (NSCLC). The study aimed to probe the function of circ_0017956 in NSCLC development. The expression of circ_0017956, microRNA (miR)-515-5p and integrin subunit beta 8 (ITGB8) was gauged by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Read More

View Article and Full-Text PDF