126,908 results match your criteria reporter

Long Noncoding RNA GAS5 Targeting miR-221-3p/Cyclin-Dependent Kinase Inhibitor 2B Axis Regulates Follicular Thyroid Carcinoma Cell Cycle and Proliferation.

Pathobiology 2021 Jun 15:1-12. Epub 2021 Jun 15.

Department of Oncology, Xiangya Hospital, Central South University, Changsha, China.

Introduction: Follicular thyroid carcinoma (FTC) is more aggressive than the most common papillary thyroid carcinoma (PTC). However, the current research on FTC is less than PTC. Here, we investigated the effects of long noncoding RNA (lncRNA) GAS5 and miR-221-3p in FTC. Read More

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Identification of natural compounds extracted from crude drugs as novel inhibitors of hepatitis C virus.

Biochem Biophys Res Commun 2021 Jun 11;567:1-8. Epub 2021 Jun 11.

Department of Virology II, National Institute of Infectious Diseases, 162-8640, Tokyo, Japan. Electronic address:

Natural product-derived crude drugs are expected to yield an abundance of new drugs to treat infectious diseases. Hepatitis C virus (HCV) is an oncogenic virus that significantly impacts public health. In this study, we sought to identify anti-HCV compounds in extracts of natural products. Read More

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Propofol represses cell growth and metastasis by modulating the circNCAPG/miR-200a-3p/RAB5A axis in glioma.

World Neurosurg 2021 Jun 12. Epub 2021 Jun 12.

Department of Anesthesiology, The First Hospital of Hebei Medicine University, Shijiazhuang, Hebei,050011, P.R. China. Electronic address:

Background: Glioma is a common primary intracranial tumor, with high infiltration and aggression. Propofol (Pro) is associated with growth and metastasis in glioma. Meanwhile, circular RNA non-SMC condensin I complex subunit G (circNCAPG, hsa_circ_0007244) has been reported to be upregulated in glioma. Read More

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UCP1 expression in the mouse adrenal gland is not upregulated by thermogenic conditions.

Biochem Biophys Res Commun 2021 Jun 12;566:184-189. Epub 2021 Jun 12.

Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, 700-8558, Japan. Electronic address:

The uncoupling protein 1 (UCP1) gene is known to be highly expressed in brown adipose tissue (BAT) that functions in thermogenesis. It has been shown that UCP1 mRNA is localized to the mouse adrenal gland, but its significance remains elusive. To explore how UCP1 expression in the adrenal gland is regulated, we generated a reporter knock-in mouse in which the GFP gene was inserted into the UCP1 locus using CRISPR-Cas9 system. Read More

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Kaempferol inhibits benign prostatic hyperplasia by resisting the action of androgen.

Eur J Pharmacol 2021 Jun 12:174251. Epub 2021 Jun 12.

Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Poyang Lake Road 10, Tianjin 301617, China; Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Poyang Lake Road 10, Tianjin 301617, China; Laboratory of Pharmacology of TCM Formulae Co-Constructed By the Province-Ministry, Tianjin University of TCM, Poyang Lake Road 10, Tianjin 301617, China. Electronic address:

Kaempferol is a natural compound that inhibits tumor development in androgenic related prostate cancer. However, it is still not clear about its phyto-androgenic activity and whether it suppresses testosterone-induced benign prostatic hyperplasia (BPH) development. In this study, molecular docking, cellular immunofluorescence staining, chromatin immunoprecipitation and dual luciferase reporter assay were performed to investigate the androgenic activity of kaempferol. Read More

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Self-Assembled Nanobodies as Selectively Targeted, Nanostructured, and Multivalent Materials.

ACS Appl Mater Interfaces 2021 Jun 15. Epub 2021 Jun 15.

Laboratory for Environmental and Life Sciences, University of Nova Gorica Nova Gorica 5000, Slovenia.

Nanobodies represent valuable tools in advanced therapeutic strategies but their small size (∼2.5 × ∼ 4 nm) and limited valence for interactions might pose restrictions for applications, especially regarding their modest capacity for multivalent and cooperative interaction. In this work, modular protein constructs have been designed, in which nanobodies are fused to protein domains to provide further functionalities and to favor oligomerization into stable self-assembled nanoparticles. Read More

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Programmable, Multiplexed DNA Circuits Supporting Clinically Relevant, Electrochemical Antibody Detection.

ACS Sens 2021 Jun 15. Epub 2021 Jun 15.

Department of Chemical Science and Technologies, University of Rome, Tor Vergata, 00133 Rome, Italy.

Current health emergencies have highlighted the need to have rapid, sensitive, and convenient platforms for the detection of specific antibodies. In response, we report here the design of an electrochemical DNA circuit that responds quantitatively to multiple specific antibodies. The approach employs synthetic antigen-conjugated nucleic acid strands that are rationally designed to induce a strand displacement reaction and release a redox reporter-modified strand upon the recognition of a specific target antibody. Read More

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Homogeneous Visual and Fluorescence Detection of Circulating Tumor Cells in Clinical Samples Selective Recognition Reaction and Enzyme-Free Amplification.

ACS Nano 2021 Jun 15. Epub 2021 Jun 15.

Department of Laboratory Medicine, State Key Laboratory of Biotherapy and Cancer Center, Med+X Center for Manufacturing, West China Precision Medicine Industrial Technology Institute, Department of Urology, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan 610041, China.

Here we report a simple all-nucleic-acid enzyme-free catalyzed hairpin assembly assisted amplification strategy with quantum dots (QDs) as the nanoscale signal reporter for homogeneous visual and fluorescent detection of A549 lung cancer cells from clinical blood samples. This work was based on the phenomenon that CdTe QDs can selectively recognize Ag and C-Ag-C and by using mucin 1 as the circulating tumor cells (CTCs) marker and aptamer as the recognition probe. Under optimized conditions, the limits of detections as low as 0. Read More

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MicroRNA-9 inhibits proliferation and progression in retinoblastoma cells by targeting PTEN.

Genes Genomics 2021 Jun 15. Epub 2021 Jun 15.

Department of Anesthesia Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, China.

Background: Retinoblastoma (RB) is the most prevalent primary intraocular malignancy, which commonly occurs during infant and childhood.

Objective: Our study aimed to investigate whether microRNA-9 (miR-9) could regulate RB cells and its mechanism.

Methods: qRT-PCR analysis was used to detect the expression of miR-9. Read More

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Vascular endothelial cell-derived exosomal miR-30a-5p inhibits lung adenocarcinoma malignant progression by targeting CCNE2.

Carcinogenesis 2021 Jun 15. Epub 2021 Jun 15.

Department of Oncology, The First Affiliated Hospital of Soochow University, Jiangsu, China, Department of Thoracic Surgery, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Zhejiang Cancer Hospital, Hangzhou, Zhejiang, China, Institute of Cancer and Basic Medicine (IBMC) Chinese Academy of Sciences, Gongshu District, Hangzhou, China.

This study tried to explore the molecular mechanism underlying progression of lung adenocarcinoma (LUAD), and discuss the extracellular communication between cancer cells and vascular endothelial cells. Roughly, differential analysis was carried out to note that miR-30a-5p was lowly expressed in LUAD while CCNE2 was highly expressed. Cell functional experiments demonstrated that overexpressed miR-30a-5p led to suppressed cell abilities in proliferation, migration and invasion. Read More

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Correlation between single nucleotide polymorphisms in the 3 primer untranslated region of PTX3 and the risk of essential hypertension: A case-control study.

Medicine (Baltimore) 2021 Jun;100(24):e25937

Department of Clinical Laboratory, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.

Abstract: The aim of this study was to investigate the correlation between single-nucleotide polymorphisms (SNPs) in the 3 primer of untranslated region (3'UTR) of the Pentraxin 3 (PTX3) gene and the risk of essential hypertension (EHT).PTX3 genotypes, rs2614, rs111451363, and rs73158510 locus, were found in 260 patients with EHT and 260 healthy controls. Quantitative real-time polymerase chain reaction was used to detect plasma hsa-miR-4766-5p levels. Read More

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The AAV9 variant capsid AAV-F mediates widespread transgene expression in non-human primate spinal cord after intrathecal administration.

Hum Gene Ther 2021 Jun 15. Epub 2021 Jun 15.

Massachusetts General Hospital, 2348, Boston, Massachusetts, United States;

Intrathecal delivery of AAV9 into the subarachnoid space has been shown to transduce spinal cord and brain and be less affected by pre-existing antibodies, which are lower in cerebral spinal fluid (CSF). Still, efficiency of transduction needs to be improved. Recently we identified a new capsid from a library selection in mice, called AAV-F, that allowed robust transduction of the spinal cord gray matter after lumbar injection. Read More

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HOXD Antisense Growth-Associated Long Noncoding RNA Promotes Triple-Negative Breast Cancer Progression by Activating Wnt Signaling Pathway.

J Breast Cancer 2021 Apr 28. Epub 2021 Apr 28.

Department of Breast Surgery, Zhengxing Hospital, Zhangzhou, China.

Purpose: Triple-negative breast cancer (TNBC) is the most lethal subtype of breast cancer owing to high heterogeneity, aggressive nature, and lack of treatment options, which has a substantial deleterious effect on patients' lives. HOXD antisense growth-associated long noncoding RNA (lncRNA) (HAGLR) plays tumor-promoting roles in many cancers. In this study, we aimed to explore the role of HAGLR in TNBC. Read More

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Detecting FRET in living cells by conventional and spectral flow cytometry.

Cytometry A 2021 Jun 14. Epub 2021 Jun 14.

Department of Lymphoma and Myeloma, The University of Texas-MD Anderson Cancer Center, Houston, TX, USA.

Assays based on Förster resonance energy transfer (FRET) can be used to study many processes in cell biology. Although this is most often done with microscopy for fluorescence detection, we report two ways to measure FRET in living cells by flow cytometry. Using a conventional flow cytometer and the "3-cube method" for intensity-based calculation of FRET efficiency, we measured the enzymatic activity of specific kinases in cells expressing a genetically-encoded reporter. Read More

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miR-141-5p suppresses vascular smooth muscle cell inflammation, proliferation, and migration via inhibiting the HMGB1/NF-κB pathway.

J Biochem Mol Toxicol 2021 Jun 14:e22828. Epub 2021 Jun 14.

Department of Pharmacy, Shanxi Cancer Hospital, Taiyuan, Shanxi, China.

MicroRNAs (miRNAs) have been identified as significant modulators in the pathogenesis of atherosclerosis (AS). Additionally, the dysregulation of vascular smooth muscle cells (VSMCs) is a crucial biological event during AS. Our study aimed to explore the functional roles and molecular mechanisms of miR-141-5p in VSMCs dysfunction. Read More

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The ER membrane protein complex subunit Emc3 controls angiogenesis via the FZD4/WNT signaling axis.

Sci China Life Sci 2021 Jun 10. Epub 2021 Jun 10.

Sichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Medical Genetics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China.

The endoplasmic reticulum (ER) membrane protein complex (EMC) regulates the synthesis and quality control of membrane proteins with multiple transmembrane domains. One of the membrane spanning subunits, EMC3, is a core member of the EMC complex that provides essential hydrophilic vestibule for substrate insertion. Here, we show that the EMC subunit Emc3 plays critical roles in the retinal vascular angiogenesis by regulating Norrin/Wnt signaling. Read More

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Potential transmission chains of variant B.1.1.7 and co-mutations of SARS-CoV-2.

Cell Discov 2021 Jun 15;7(1):44. Epub 2021 Jun 15.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.

The presence of SARS-CoV-2 mutants, including the emerging variant B.1.1. Read More

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Genes with 5' terminal oligopyrimidine tracts preferentially escape global suppression of translation by the SARS-CoV-2 NSP1 protein.

RNA 2021 Jun 14. Epub 2021 Jun 14.

University of Texas at Austin

Viruses rely on the host translation machinery to synthesize their own proteins. Consequently, they have evolved varied mechanisms to co-opt host translation for their survival. SARS-CoV-2 relies on a non-structural protein, Nsp1, for shutting down host translation. Read More

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CircASXL1 knockdown represses the progression of colorectal cancer by downregulating GRIK3 expression by sponging miR-1205.

World J Surg Oncol 2021 Jun 14;19(1):176. Epub 2021 Jun 14.

Department of General Surgery, Shanghai Fengxian Central Hospital, No. 6600, Nanfeng Road, Nanqiao New Town, Fengxian District, Shanghai, 201400, China.

Background: Colorectal cancer (CRC) is a common aggressive tumor that poses a heavy burden to human health. An increasing number of studies have reported that circular RNA (circRNA) is involved in the progression of CRC. In this study, the special profiles of circASXL1 (circ_0001136) in CRC progression were revealed. Read More

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Long non-coding RNA CDKN2B-AS1 enhances LPS-induced apoptotic and inflammatory damages in human lung epithelial cells via regulating the miR-140-5p/TGFBR2/Smad3 signal network.

BMC Pulm Med 2021 Jun 14;21(1):200. Epub 2021 Jun 14.

Department of Intensive Care Unit, The Second Clinical Medical College, Jingzhou Central Hospital, Yangtze University, No. 1 Renmin Road, Jingzhou District, Jingzhou, 434000, Hubei, China.

Background: Sepsis is a complicated disease with systemic inflammation or organ dysfunction, and it is the leading cause of acute lung injury (ALI). Long non-coding RNAs (lncRNAs) have played important roles in the pathogenesis of sepsis. This study was designed to explore the biological function and regulatory mechanism of cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1) in lipopolysaccharide (LPS)-induced lung injury. Read More

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Long non-coding RNA AFAP1-AS1 facilitates prostate cancer progression by regulating miR-15b/IGF1R axis.

Curr Pharm Des 2021 Jun 11. Epub 2021 Jun 11.

Department of Urology, Tongji Hospital, Tongji University of Medicine, 389 Xincun Road, Putuo, Shanghai, 200065, China.

Background: Prostate cancer (PCa) is a commonly diagnosed malignant cancer and is the second highest cause of cancer related death in men worldwide. Enzalutamide is the second-generation inhibitor of androgen receptor signaling and is the fundamental drug for the treatment of advanced PCa. However, the disease will eventually progress to metastatic castration-resistant prostate cancer (CRPC) and aggressive neuroendocrine prostate cancer (NEPC) because of androgen-deprivation therapy (ADT) resistance. Read More

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therapeutic response monitoring by a self-reporting upconverting covalent organic framework nanoplatform.

Chem Sci 2019 Dec 3;11(5):1299-1306. Epub 2019 Dec 3.

Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University Changsha Hunan 410082 China

The real-time and monitoring of reactive oxygen species (ROS) generation is critical for minimizing the nonspecific damage derived from the high doses of ROS required during the photodynamic therapy (PDT) process. However, phototherapeutic agents that can generate ROS-related imaging signals during PDT are rare, hampering the facile prediction of the future therapeutic outcome. Herein, we develop an upconverting covalent organic framework (COF) nanoplatform a core-mediated strategy and further functionalized it with a singlet oxygen reporter for the efficient near-infrared activated and self-reporting of PDT. Read More

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December 2019

miRNA-483-5p Targets HDCA4 to Regulate Renal Tubular Damage in Diabetic Nephropathy.

Horm Metab Res 2021 Jun 14. Epub 2021 Jun 14.

Department of Science and Technology, Heilongjiang University of Chinese Medicine, Heilongjiang, China.

This study was designed to evaluate the diagnostic value of miR-483-5p in diabetic nephropathy (DN), and its effect and mechanism on apoptosis and inflammation of human proximal renal tubular cells (HK2) induced by high glucose (HG). Thirty healthy controls, 30 types 2 diabetes mellitus (T2DM) patients, and 28 DN patients were enrolled. miR-483-5p mRNA levels in serum were analyzed by RT-qPCR assays. Read More

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Cowpea mosaic virus stimulates antitumor immunity through recognition by multiple MYD88-dependent toll-like receptors.

Biomaterials 2021 May 25;275:120914. Epub 2021 May 25.

Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH, 03755, United States; Norris Cotton Cancer Center, Geisel School of Medicine, Dartmouth Hitchcock Medical System, Lebanon, NH, 03756, United States. Electronic address:

Cowpea mosaic virus (CPMV), a non-enveloped plant virus, and empty CPMV (eCPMV), a virus-like particle (VLP) composed of CPMV capsid without nucleic acids, are potent in situ cancer vaccines when administered intratumorally (I.T.). Read More

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Expression of long non-coding RNA LUCAT1 in patients with chronic obstructive pulmonary disease and its potential functions in regulating cigarette smoke extract-induced 16HBE cell proliferation and apoptosis.

J Clin Lab Anal 2021 Jun 14:e23823. Epub 2021 Jun 14.

Department of Blood Transfusion, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Chronic obstructive pulmonary disease (COPD), characterized by persistent airflow limitation, was a disease mediated by a combination of inflammatory factors, immune cells, and immune mediators. COPD was an inflammatory and autoimmune disease involving T-lymphocytes triggered by cigarette smoke and other factors that progressively affected the bronchi, lung parenchyma, and pulmonary blood vessels. LncRNAs were reported to be implicated in COPD pathogenesis and development. Read More

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A plasmid toolbox for controlled gene expression across the Proteobacteria.

Nucleic Acids Res 2021 Jun 14. Epub 2021 Jun 14.

Dept. of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32603, USA.

Controlled gene expression is fundamental for the study of gene function and our ability to engineer bacteria. However, there is currently no easy-to-use genetics toolbox that enables controlled gene expression in a wide range of diverse species. To facilitate the development of genetics systems in a fast, easy, and standardized manner, we constructed and tested a plasmid assembly toolbox that will enable the identification of well-regulated promoters in many Proteobacteria and potentially beyond. Read More

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Study of the host specificity of PB1-F2-associated virulence.

Virulence 2021 Dec;12(1):1647-1660

Université Paris-Saclay, INRAE, UVSQ, UMR892 VIM, Jouy-en-Josas, France.

Influenza A viruses cause important diseases in both human and animal. The PB1-F2 protein is a virulence factor expressed by some influenza viruses. Its deleterious action for the infected host is mostly described in mammals, while the available information is scarce in avian hosts. Read More

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December 2021

Combining Cell Envelope Stress Reporter Assays in a Screening Approach to Identify BAM Complex Inhibitors.

ACS Infect Dis 2021 Jun 14. Epub 2021 Jun 14.

Department of Molecular Microbiology, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit, De Boelelaan 1085, 1081HV Amsterdam, The Netherlands.

The development of new antibiotics is particularly problematic in Gram-negative bacteria due to the presence of the outer membrane (OM), which serves as a permeability barrier. Recently, the β-barrel assembly machine (BAM), located in the OM and responsible for β-barrel type OM protein (OMP) assembly, has been validated as a novel target for antibiotics. Here, we identified potential BAM complex inhibitors using a screening approach that reports on cell envelope σ and Rcs stress in . Read More

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HDAC5 promotes intestinal sepsis via the Ghrelin/E2F1/NF-κB axis.

FASEB J 2021 Jul;35(7):e21368

Department of Respiratory, the First Hospital of Lanzhou University (the First School of Clinical Medicine), Lanzhou, P.R. China.

In the current study, we sought to determine the roles of histone deacetylase 5 (HDAC5) on the promotion of intestinal sepsis in a mouse model. Dual luciferase reporter gene assay was used to determine the binding relationship between HDAC5 and Ghrelin. Cecal ligation and puncture (CLP) was used as an animal model of intestinal sepsis. Read More

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MicroRNA-1178-3p suppresses the growth of hepatocellular carcinoma by regulating transducin (beta)-like 1 X-linked receptor 1.

Hum Cell 2021 Jun 14. Epub 2021 Jun 14.

Department of Hepatobiliary Surgery and Organ Transplantation, First Hospital of China Medical University, 155 Nanjing Street, Heping District, Shenyang, 110000, Liaoning, China.

MicroRNAs (miRNAs) are implicated in various cancer-relevant cellular processes, including cell proliferation, migration, invasion, and angiogenesis. However, the function of miRNAs in hepatocellular cancer (HCC) has not been fully clarified. This study aimed to investigate the role of miR-1178-3p in HCC metastasis and try to reveal the potential mechanism. Read More

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