254,070 results match your criteria regulatory gene

RIPK1 regulates cell function and death mediated by UVB radiation and TNF-α.

Mol Immunol 2021 May 5;135:304-311. Epub 2021 May 5.

College of Pharmaceutical Sciences, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China. Electronic address:

The RIP family plays a key role in mediating cell inflammation, oxidative stress and death. Among them, RIPK1, as an important regulatory factor in the upstream of the NF-κB pathway, is involved in multiple pathways of cell inflammation and death. Epidermal cells constitute the outermost barrier of the human body. Read More

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PsrA positively regulates the unsaturated fatty acid synthesis operon fabAB in Azotobacter vinelandii.

Microbiol Res 2021 Apr 29;249:126775. Epub 2021 Apr 29.

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. Electronic address:

In Pseudomonas spp. PsrA, a transcriptional activator of the rpoS gene, regulates fatty acid catabolism by repressing the fadBA5 β-oxidation operon. In Azotobacter vinelandii, a soil bacterium closely related to Pseudomonas species, PsrA is also an activator of rpoS expression, although its participation in the regulation of lipid metabolism has not been analyzed. Read More

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The miR166- SlHB15A Regulatory Module controls Ovule Development and Parthenocarpic Fruit Set under Adverse Temperatures in Tomato.

Mol Plant 2021 May 5. Epub 2021 May 5.

Institute of Plant Sciences Paris-Saclay, INRAE, CNRS, Université Paris-Saclay, Orsay 91405, France. Electronic address:

Fruit set is inhibited by adverse temperatures, with consequences on yield. We isolated a tomato mutant producing fruits under non-permissive hot temperatures and identified the causal gene as SlHB15A, belonging to class-III homeodomain leucine-zipper transcription factors (HD-ZipIII). SlHB15A loss-of-function mutants display aberrant ovule development that mimics transcriptional changes occurring in fertilized ovules and leads to parthenocarpic fruit set under optimal and non-permissive temperatures, in field and glasshouse conditions. Read More

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Engineering Pseudomonas putida for efficient aromatic conversion to bioproduct using high throughput screening in a bioreactor.

Metab Eng 2021 May 5. Epub 2021 May 5.

Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, 5885, Hollis Street, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA, USA. Electronic address:

Pseudomonas putida KT2440 is an emerging biomanufacturing host amenable for use with renewable carbon streams including aromatics such as para-coumarate. We used a pooled transposon library disrupting nearly all (4,778) non-essential genes to characterize this microbe under common stirred-tank bioreactor parameters with quantitative fitness assays. Assessing differential fitness values by monitoring changes in mutant strain abundance identified 33 gene mutants with improved fitness across multiple stir-tank bioreactor formats. Read More

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Identification of hub genes and signaling pathways related to gastric cells infected by Helicobacter pylori.

Microb Pathog 2021 May 5:104932. Epub 2021 May 5.

Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China; Department of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China; Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China; Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China. Electronic address:

Background: Helicobacter pylori is a pathogen involved in several gastroduodenal diseases, whose infection mechanisms have not been completely confirmed. To study the specific mechanism of gastropathy caused by H. pylori, we analyzed the gene microarray of gastric mucosa and gastric cells infected by H. Read More

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Assessment of progesterone synthesis and its regulation role on dihydrotestosterone secretion in sheep epididymis.

Gene 2021 May 5:145699. Epub 2021 May 5.

Animal Science and Technology College, Gansu Agricultural University, Lanzhou, China. Electronic address:

Progesterone (P4) is an anti-androgen compound whose role in sperm maturation and functionality remains unclear in sheep. Here, we aimed to investigate the regulation mechanism of P4 on the epididymal secretion of dihydrotestosterone (DHT). To this end, we performed enzyme-linked immunosorbent assays, immunohistochemical staining, western blotting, and quantitative real-time polymerase chain reaction to detect P4 concentration as well as StAR, P450scc, and 3β-HSD expression in sheep epididymis. Read More

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EHD3 positively regulated by NR5A1 participates in testosterone synthesis via endocytosis.

Life Sci 2021 May 5:119570. Epub 2021 May 5.

NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Pharmacy, Fudan University, Shanghai 200032, China; NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai 200032, China. Electronic address:

Aims: Increasing evidence has shown that hormone secretion is regulated by endocytosis. Eps15 homology domain-containing protein 3 (EHD3) is an endocytic-trafficking regulatory protein, but whether EHD3 is associated with testosterone secretion is not clear. This work aims to explore the role of EHD3 in testosterone synthesis. Read More

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A cis-regulatory atlas in maize at single-cell resolution.

Cell 2021 Apr 30. Epub 2021 Apr 30.

Department of Genetics, University of Georgia, Athens, GA 30602, USA. Electronic address:

cis-regulatory elements (CREs) encode the genomic blueprints of spatiotemporal gene expression programs enabling highly specialized cell functions. Using single-cell genomics in six maize organs, we determined the cis- and trans-regulatory factors defining diverse cell identities and coordinating chromatin organization by profiling transcription factor (TF) combinatorics, identifying TFs with non-cell-autonomous activity, and uncovering TFs underlying higher-order chromatin interactions. Cell-type-specific CREs were enriched for enhancer activity and within unmethylated long terminal repeat retrotransposons. Read More

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Retinoic acid stimulates transcription of the rat SHARP-2 gene via multiple pathways.

J Biochem 2021 May 8. Epub 2021 May 8.

Matsumoto University Graduate School of Health Science, 2095-1 Niimura, Matsumoto, Nagano 390-1295, Japan.

Members of the enhancer of split- and hairy-related protein (SHARP) family, SHARP-1 and SHARP-2, are basic helix-loop-helix transcriptional repressors and belong to the clock genes. In this study, an effect of retinoic acid (RA) on the SHARP family gene expression in the differentiated cells was examined. RA rapidly and temporarily induced the SHARP-2 mRNA expression in hepatic H4IIE cells. Read More

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Improved genome assembly and pan-genome provide key insights on eggplant domestication and breeding.

Plant J 2021 May 8. Epub 2021 May 8.

ENEA, Casaccia Res Ctr, Via Anguillarese 301, 00123, Rome, Italy.

Eggplant (Solanum melongena L.) is an important horticultural crop and one of the most widely grown vegetables from the Solanaceae family. It was domesticated from a wild, prickly progenitor carrying small, round, non-anthocyanic fruits. Read More

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A structural intermediate pre-organizes the add adenine riboswitch for ligand recognition.

Nucleic Acids Res 2021 May 8. Epub 2021 May 8.

Department of Biology, Faculty of Science, Université de Sherbrooke, Sherbrooke, Quebec J1K 2R1, Canada.

Riboswitches are RNA sequences that regulate gene expression by undergoing structural changes upon the specific binding of cellular metabolites. Crystal structures of purine-sensing riboswitches have revealed an intricate network of interactions surrounding the ligand in the bound complex. The mechanistic details about how the aptamer folding pathway is involved in the formation of the metabolite binding site have been previously shown to be highly important for the riboswitch regulatory activity. Read More

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The global regulator Hfq exhibits far more extensive and intensive regulation than Crc in Pseudomonas protegens H78.

Mol Plant Pathol 2021 May 8. Epub 2021 May 8.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

The biocontrol rhizobacterium Pseudomonas protegens H78 can produce a large array of antimicrobial secondary metabolites, including pyoluteorin (Plt), 2,4-diacetylphloroglucinol (DAPG), and pyrrolnitrin (Prn). Our preliminary study showed that the biosynthesis of antibiotics including Plt is activated by the RNA chaperone Hfq in P. protegens H78. Read More

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Development of a multiplex assay to determine the expression of mitochondrial genes in human skeletal muscle.

Exp Physiol 2021 May 7. Epub 2021 May 7.

Physical Education and Sports Sciences, University of Limerick, Limerick, Ireland.

New Findings: What is the central question of this study? Description and testing of a custom-designed multiplex gene expression assay to quantitate expression levels of a targeted group of mitochondrial genes in human skeletal muscle. What is the main finding and its importance? This study describes the development of a custom-designed GeXP multiplex assay and demonstrates the ability to accurately quantitate expression of a targeted set of mitochondrial genes in human skeletal muscle, while holding distinct methodological and practical advantages over other commonly used quantitation methods.

Abstract: Skeletal muscle is an important endocrine tissue demonstrating plasticity in response to external stimuli, including exercise and nutrition. Read More

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Integrative analysis of miRNAs and mRNAs revealed regulation of lipid metabolism in dairy cattle.

Funct Integr Genomics 2021 May 7. Epub 2021 May 7.

College of Animal Science, Jilin University, Changchun, 130062, People's Republic of China.

Lipid metabolism in bovine mammary epithelial cells has been the primary focus of the research of milk fat percentage of dairy cattle. Functional microRNAs can affect lipid metabolism by regulating the expression of candidate genes. The purpose of the study was to screen and identify differentially expressed miRNAs, candidate genes, and co-regulatory pathways related to the metabolism of milk fat. Read More

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Recent Research Advances in Small Regulatory RNAs in Streptococcus.

Curr Microbiol 2021 May 7. Epub 2021 May 7.

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Small non-coding RNAs (sRNAs) are a class of regulatory RNAs 20-500 nucleotides in length, which have recently been discovered in prokaryotic organisms. sRNAs are key regulators in many biological processes, such as sensing various environmental changes and regulating intracellular gene expression through binding target mRNAs or proteins. Bacterial sRNAs have recently been rapidly mined, thus providing new insights into the regulatory network of biological functions in prokaryotes. Read More

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A transcriptional hub integrating gibberellin-brassinosteroid signals to promote seed germination in Arabidopsis.

J Exp Bot 2021 May 8. Epub 2021 May 8.

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China.

Seed germination is regulated by multiple phytohormones, including Gibberellins (GAs) and Brassinosteroids (BRs); however, the molecular mechanism underlying GA and BR co-induced seed germination is not well elucidated. We demonstrated that BRs induce seed germination through promoting testa and endosperm rupture in Arabidopsis. BRs promote cell elongation, rather than cell division, at the hypocotyl-radicle transition region of embryonic axis during endosperm rupture. Read More

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Allele-specific DNA methylation maps in monozygotic twins discordant for psychiatric disorders reveal that disease-associated switching at the EIPR1 regulatory loci modulates neural function.

Mol Psychiatry 2021 May 7. Epub 2021 May 7.

Department of Medical Genetics, School of Basic Medical Sciences, and Guangdong Technology and Engineering Research Center for Molecular Diagnostics of Human Genetic Diseases, Southern Medical University, Guangzhou, Guangdong, China.

The non-Mendelian features of phenotypic variations within monozygotic twins are likely complicated by environmental modifiers of genetic effects that have yet to be elucidated. Here, we performed methylome and genome analyses of blood DNA from psychiatric disorder-discordant monozygotic twins to study how allele-specific methylation (ASM) mediates phenotypic variations. We identified that thousands of genetic variants with ASM imbalances exhibit phenotypic variation-associated switching at regulatory loci. Read More

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Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in .

Proc Natl Acad Sci U S A 2021 May;118(19)

Faculty of Science, Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan;

Nitrogen (N) is an essential nutrient that affects multiple plant developmental processes, including flowering. As flowering requires resources to develop sink tissues for reproduction, nutrient availability is tightly linked to this process. Low N levels accelerate floral transition; however, the molecular mechanisms underlying this response are not well understood. Read More

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GCN4 regulates secondary metabolism through activating antioxidant genes expression under nitrogen limitation in .

Appl Environ Microbiol 2021 May 7. Epub 2021 May 7.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture; Microbiology Department, College of Life Sciences, Agricultural University, Jiangsu, Nanjing 210095, People's Republic of China.

Nitrogen limitation has been widely reported to affect the growth and development of fungi, and the transcription factor GCN4 (general control nonderepressible 4) is involved in nitrogen restriction. Here, we found that nitrogen limitation highly induced the expression of GCN4 and promoted the synthesis of ganoderic acid (GA), an important secondary metabolite in The activated GCN4 is involved in regulating GA biosynthesis. Besides, the accumulation of ROS is also affecting the synthesis of GA under nitrogen restrictions. Read More

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Cancer type-specific alterations in actin genes: Worth a closer look?

Int Rev Cell Mol Biol 2021 30;360:133-184. Epub 2021 Mar 30.

Department of Biomolecular Medicine, Ghent University, Gent, Belgium.

Actins form a strongly conserved family of proteins that are central to the functioning of the actin cytoskeleton partaking in natural processes such as cell division, adhesion, contraction and migration. These processes, however, also occur during the various phases of cancer progression. Yet, surprisingly, alterations in the six human actin genes in cancer studies have received little attention and the focus was mostly on deregulated expression levels of actins and even more so of actin-binding or regulatory proteins. Read More

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Dual RNAseq highlights the kinetics of skin microbiome and fish host responsiveness to bacterial infection.

Anim Microbiome 2021 May 7;3(1):35. Epub 2021 May 7.

Ifremer, IRD, Institut Louis-Malardé, Univ Polynésie Française, EIO, F-98719 Taravao, Tahiti, Polynésie Française.

Background: Tenacibaculum maritimum is a fish pathogen known for causing serious damage to a broad range of wild and farmed marine fish populations worldwide. The recently sequenced genome of T. maritimum strain NCIMB 2154 provided unprecedented information on the possible molecular mechanisms involved in the virulence of this species. Read More

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ROC1 promotes the malignant progression of bladder cancer by regulating p-IκBα/NF-κB signaling.

J Exp Clin Cancer Res 2021 May 7;40(1):158. Epub 2021 May 7.

Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No.100 Haining Road, Hongkou District, Shanghai, 200080, China.

Background: Regulator of cullins 1 (ROC1) is an important catalytic subunit of cullin-RING E3 ligase. Nuclear factor-kappa B (NF-κB) signaling is closely related to tumor invasion and metastasis. Earlier, we reported that ROC1 was associated with a poor prognosis in patients with bladder cancer (BCa). Read More

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A prognostic model based on seven immune-related genes predicts the overall survival of patients with hepatocellular carcinoma.

BioData Min 2021 May 7;14(1):29. Epub 2021 May 7.

Department of Oncology, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China.

Background: Hepatocellular carcinoma (HCC) is a disease with a high incidence and a poor prognosis. Growing amounts of evidence have shown that the immune system plays a critical role in the biological processes of HCC such as progression, recurrence, and metastasis, and some have discussed using it as a weapon against a variety of cancers. However, the impact of immune-related genes (IRGs) on the prognosis of HCC remains unclear. Read More

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MOCCA: a flexible suite for modelling DNA sequence motif occurrence combinatorics.

BMC Bioinformatics 2021 May 7;22(1):234. Epub 2021 May 7.

Department of Biology, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099, Berlin, Germany.

Background: Cis-regulatory elements (CREs) are DNA sequence segments that regulate gene expression. Among CREs are promoters, enhancers, Boundary Elements (BEs) and Polycomb Response Elements (PREs), all of which are enriched in specific sequence motifs that form particular occurrence landscapes. We have recently introduced a hierarchical machine learning approach (SVM-MOCCA) in which Support Vector Machines (SVMs) are applied on the level of individual motif occurrences, modelling local sequence composition, and then combined for the prediction of whole regulatory elements. Read More

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Excretion from long glandular trichomes contributes to alleviation of cadmium toxicity in Nicotiana tabacum.

Environ Pollut 2021 Apr 29;285:117184. Epub 2021 Apr 29.

Key Laboratory for Cultivation of Tobacco Industry, College of Tobacco Science, Henan Agricultural University, Zhengzhou, 450002, China. Electronic address:

The B-type cyclin gene, CycB2, serves as a negative regulator of glandular trichome initiation. Through targeted knockout of NtCycB2 in Nicotiana tabacum cv. K326 using the CRISPR/Cas9 system, we created a variety, HK326, which exhibits significantly increased density and larger glandular heads of long glandular trichomes. Read More

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Characterization of the asexual developmental genes brlA and wetA in Monascus ruber M7.

Fungal Genet Biol 2021 May 4:103564. Epub 2021 May 4.

Hubei International Scientific and Technological Cooperation Base of Traditional Fermented Foods, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, China. Electronic address:

Monascus spp. are widely used in the production of monacolin K and food- grade pigments in East Asia. In Aspergillus species, the three transcription factors BrlA→AbaA→WetA sequentially function as the central activators of asexual development (conidiation), leading to the formation of conidiophores. Read More

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Melatonin prevents blood-retinal barrier breakdown and mitochondrial dysfunction in high glucose and hypoxia-induced in vitro diabetic macular edema model.

Toxicol In Vitro 2021 May 4:105191. Epub 2021 May 4.

Trakya University, Department of Medicinal Biology, Edirne, Turkey.

Diabetic macular edema (DME) is a leading cause of blindness in diabetic retinopathy. Prolonged hyperglycemia plus hypoxia contributes to DME pathogenesis. Retinal pigmented epithelial cells comprise the outer blood-retinal barrier and are essential for maintaining physiological functioning of the retina. Read More

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CircRNA_0026344 via exosomal miR-21 regulation of Smad7 is involved in aberrant cross-talk of epithelium-fibroblasts during cigarette smoke-induced pulmonary fibrosis.

Toxicol Lett 2021 May 4. Epub 2021 May 4.

School of Public Health, Southwest Medical University, Luzhou, 646000, Sichuan, People's Republic of China. Electronic address:

For smoking-induced pulmonary fibrosis (PF), a serious disease endangering human health, there is no effective clinical treatment. Aberrant epithelium-fibroblast cross-talk is involved in formation of the excessive extracellular matrix (ECM) that contributes to PF. Circular RNAs have been associated with various pulmonary diseases. Read More

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Recent achievements and doors opened for coccidian parasite research and development through transcriptomics of enteric sexual stages.

Mol Biochem Parasitol 2021 May 4:111373. Epub 2021 May 4.

School of Life Sciences, University of Technology Sydney, Ultimo, NSW 2007, Australia; Research School of Biology, Australian National University, Canberra, ACT 0200, Australia. Electronic address:

The Coccidia is the largest group of parasites within the Apicomplexa, a phylum of unicellular, obligate parasites characterized by the possession of an apical complex of organelles and structures in the asexual stages of their life cycles, as well as by a sexual reproductive phase that occurs enterically in host animals. Coccidian sexual reproduction involves morphologically distinct microgametes and macrogametes that combine to form a diploid zygote and, ultimately, following meiosis and mitosis, haploid, infectious sporozoites, inside sporocysts within an oocyst. Recent transcriptomic analyses have identified genes involved in coccidian sexual stage development and reproduction, including genes encoding for microgamete- and macrogamete-specific proteins with roles in gamete motility, fusion and fertilization, and in the formation of the resilient oocyst wall that allows coccidians to persist for long periods in the environment. Read More

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TIRR inhibits the 53BP1-p53 complex to alter cell-fate programs.

Mol Cell 2021 Apr 29. Epub 2021 Apr 29.

Department of Radiation Oncology, Dana-Farber Cancer Institute, Boston, MA 02115, USA; Department of Biological Chemistry & Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA. Electronic address:

53BP1 influences genome stability via two independent mechanisms: (1) regulating DNA double-strand break (DSB) repair and (2) enhancing p53 activity. We discovered a protein, Tudor-interacting repair regulator (TIRR), that associates with the 53BP1 Tudor domain and prevents its recruitment to DSBs. Here, we elucidate how TIRR affects 53BP1 function beyond its recruitment to DSBs and biochemically links the two distinct roles of 53BP1. Read More

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