148,863 results match your criteria regulation small

Comprehensive analysis of BTN3A1 in cancers: mining of omics data and validation in patient samples and cellular models.

FEBS Open Bio 2021 Jul 22. Epub 2021 Jul 22.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Butyrophilin 3A1 (BTN3A1), a major histocompatibility complex (MHC)-associated gene that encodes a membrane protein with 2 extracellular immunoglobulin domains and an intracellular B30.2 domain, is critical in T-cell activation and adaptive immune response. Here, the expression of BTN3A1 in cancers was analyzed in 8 databases comprising 86,733 patients of 33 cancers and the findings were validated in patient samples and cell models. Read More

View Article and Full-Text PDF

Making large changes or small changes to prevent weight gain in young adulthood: which is preferred and by whom?

Transl Behav Med 2021 Jul 22. Epub 2021 Jul 22.

Alpert Medical School of Brown University, Miriam Hospital, Providence, RI 02903, USA.

Knowledge of participant treatment preferences can inform decision-making regarding treatment dissemination and future participant adoption. To compare participant perceptions of two evidence-based approaches for weight gain prevention in young adults to identify the intervention with the greatest likelihood of adoption. As part of a randomized trial (Study of Novel Approaches to Weight Gain Prevention [SNAP]; n = 599) testing weight gain prevention interventions in young adults (18-35 years), individuals assigned to self-regulation interventions using either large changes or small changes reported on perceived personal effectiveness and difficulty of treatment over 3 years. Read More

View Article and Full-Text PDF

Therapist initial attachment style, changes in attachment style during training, and client outcome in psychodynamic psychotherapy.

J Couns Psychol 2021 Jul 22. Epub 2021 Jul 22.

Department of Psychology.

This study extended the cross-sectional therapist attachment literature by examining longitudinal changes of therapist attachment avoidance and anxiety in relation to client treatment outcome. Data consisted of 942 Outcome Questionnaire-45 assessments (Lambert et al., 1996, 2004) of 213 clients working with 30 therapists from a university clinic that provided psychodynamically/interpersonally oriented individual therapy, and yearly therapist self-report of attachment styles using the Experience in Close Relationships Scale (Brennan et al. Read More

View Article and Full-Text PDF

Induction and Monitoring of DNA Phase Separation in Living Cells by a Light-Switching Ruthenium Complex.

J Am Chem Soc 2021 Jul 22. Epub 2021 Jul 22.

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, State Key Laboratory of Oncology in South China, Sun Yat-Sen University, Guangzhou 510275, P. R. China.

Phase separation of DNA is involved in chromatin packing for the regulation of gene transcription. Visualization and manipulation of DNA phase separation in living cells present great challenges. Herein, we present a Ru(II) complex () with high DNA binding affinity and DNA "light-switch" behavior that can induce and monitor DNA phase separation both and in living cells. Read More

View Article and Full-Text PDF

Crowding Effects on the Structure and Dynamics of the Intrinsically Disordered Nuclear Chromatin Protein NUPR1.

Front Mol Biosci 2021 5;8:684622. Epub 2021 Jul 5.

Instituto de Biocomputación y Física de Sistemas Complejos (BIFI), Joint Units IQFR-CSIC-BIFI and GBsC-CSIC-BIFI, Universidad de Zaragoza, Zaragoza, Spain.

The intracellular environment is crowded with macromolecules, including sugars, proteins and nucleic acids. In the cytoplasm, crowding effects are capable of excluding up to 40% of the volume available to any macromolecule when compared to dilute conditions. NUPR1 is an intrinsically disordered protein (IDP) involved in cell-cycle regulation, stress-cell response, apoptosis processes, DNA binding and repair, chromatin remodeling and transcription. Read More

View Article and Full-Text PDF

E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity.

Front Cell Dev Biol 2021 5;9:671244. Epub 2021 Jul 5.

College of Medicine, Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan.

MicroRNAs (miRNAs) are small non-coding RNAs which post-transcriptionally suppress target mRNAs expression and/or translation to modulate pathophyological processes. Expression and function of miRNAs are fine-tuned by a conserved biogenesis machinery involves two RNase-dependent processing steps of miRNA maturation and the final step of miRNA-induced silencing complex (miRISC)-mediated target silencing. A functional miRISC requires Argonaute 2 (AGO2) as an essential catalytic component which plays central roles in miRISC function. Read More

View Article and Full-Text PDF

ST6GAL1 and α2-6 Sialylation Regulates IL-6 Expression and Secretion in Chronic Obstructive Pulmonary Disease.

Front Immunol 2021 5;12:693149. Epub 2021 Jul 5.

Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, The University of Alabama at Birmingham, Birmingham, AL, United States.

Chronic obstructive pulmonary disease (COPD) is a systemic disease strongly associated with cigarette smoking, airway inflammation, and acute disease exacerbations. Changes in terminal sialylation and fucosylation of asparagine (N)-linked glycans have been documented in COPD, but the role that glycosyltransferases may play in the regulation of N-linked glycans in COPD has not been fully elucidated. Recent studies suggest that modulation of ST6GAL1 (ST6 beta-galactoside alpha-2,6-sialyltransferase-1), which catalyzes terminal α2-6 sialylation of cellular proteins, may regulate inflammation and contribute to COPD phenotype(s). Read More

View Article and Full-Text PDF

EGFR activation limits the response of liver cancer to lenvatinib.

Nature 2021 Jul 21. Epub 2021 Jul 21.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Hepatocellular carcinoma (HCC)-the most common form of liver cancer-is an aggressive malignancy with few effective treatment options. Lenvatinib is a small-molecule inhibitor of multiple receptor tyrosine kinases that is used for the treatment of patients with advanced HCC, but this drug has only limited clinical benefit. Here, using a kinome-centred CRISPR-Cas9 genetic screen, we show that inhibition of epidermal growth factor receptor (EGFR) is synthetic lethal with lenvatinib in liver cancer. Read More

View Article and Full-Text PDF

Up-regulation of gasdermin C in mouse small intestine is associated with lytic cell death in enterocytes in worm-induced type 2 immunity.

Proc Natl Acad Sci U S A 2021 Jul;118(30)

Monell Chemical Senses Center, Philadelphia, PA 19104;

"Taste-like" tuft cells in the intestine trigger type 2 immunity in response to worm infection. The secretion of interleukin-13 (IL-13) from type 2 innate lymphoid cells (ILC2) represents a key step in the tuft cell-ILC2 cell-intestinal epithelial cell circuit that drives the clearance of worms from the gut via type 2 immune responses. Hallmark features of type 2 responses include tissue remodeling, such as tuft and goblet cell expansion, and villus atrophy, yet it remains unclear if additional molecular changes in the gut epithelium facilitate the clearance of worms from the gut. Read More

View Article and Full-Text PDF

[LncRNA ASB16-AS1 regulates the proliferation, migration and invasion of esophageal cancer cells by targeting miR-1258].

Zhonghua Zhong Liu Za Zhi 2021 Jul;43(7):762-768

Henan Medical Association, Zhengzhou 450000, China.

To investigate the effects of long-chain non-coding RNA ASB16 antisense RNA1 (ASB16-AS1) on the proliferation, migration and invasion of esophageal cancer cells by targeting microRNA (miR )-1258. Forty pairs of esophageal cancer tissues and matched adjacent tissues (distance of tumor margin>3 cm) resected in Xinxiang Central Hospital from May 2016 to July 2017 were collected. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expressions of ASB16-AS1 and miR-1258 in esophageal cancer tissues and adjacent tissues. Read More

View Article and Full-Text PDF

A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA.

Cell Rep 2021 Jul;36(3):109413

Hunan Key Laboratory of Economic Crops, Genetic Improvement, and Integrated Utilization, School of Life Sciences, Hunan University of Science and Technology, Xiangtan, Hunan 411201, PRC. Electronic address:

Metabolic regulation strategies have been developed to redirect metabolic fluxes to production pathways. However, it is difficult to screen out target genes that, when repressed, improve yield without affecting cell growth. Here, we report a strategy using a quorum-sensing system to control small RNA transcription, allowing cell-density-dependent repression of target genes. Read More

View Article and Full-Text PDF

Assessment of heterogeneity in collective endothelial cell behavior with multicolor clonal cell tracking to predict arteriovenous remodeling.

Cell Rep 2021 Jul;36(3):109395

The David Richmond Laboratory for Cardiovascular Development, Gene Regulation and Editing, the Centenary Institute, Camperdown, 2006 NSW, Australia; School of Life and Environmental Sciences, The University of Sydney, Camperdown, 2006 NSW, Australia. Electronic address:

Arteries and veins form in a stepwise process that combines vasculogenesis and sprouting angiogenesis. Despite extensive data on the mechanisms governing blood vessel assembly at the single-cell level, little is known about how collective cell migration contributes to the organization of the balanced distribution between arteries and veins. Here, we use an endothelial-specific zebrafish reporter, arteriobow, to label small cohorts of arterial cells and trace their progeny from early vasculogenesis throughout arteriovenous remodeling. Read More

View Article and Full-Text PDF

SUMOylation of different targets fine-tunes phytochrome signaling.

New Phytol 2021 Jul 21. Epub 2021 Jul 21.

Laboratory of Photo- and Chronobiology, Institute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network (ELKH), Szeged, H-6726, Hungary.

Plants monitor their surrounding ambient light environment by specialized photoreceptor proteins. Among them, phytochromes monitor red and far-red light. These molecules perceive photons, undergo a conformational change and regulate diverse light signaling pathways resulting in the mediation of key developmental and growth responses throughout the whole life of plants. Read More

View Article and Full-Text PDF

Bioinformatics prediction of differential miRNAs in non-small cell lung cancer.

PLoS One 2021 21;16(7):e0254854. Epub 2021 Jul 21.

Department of Pulmonary and Critical Care Medicine, University of South China Affiliated Changsha Central Hospital, Changsha City, Hunan Province, China.

Background: Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancers. The drug resistance of NSCLC has clinically increased. This study aimed to screen miRNAs associated with NSCLC using bioinformatics analysis. Read More

View Article and Full-Text PDF

Inhibition of lipid phosphatase SHIP1 expands myeloid-derived suppressor cells and attenuates rheumatoid arthritis in mice.

Am J Physiol Cell Physiol 2021 07 21. Epub 2021 Jul 21.

Division of Hematology/Oncology, Department of Medicine, Rhode Island Hospital, Warren Alpert Medical School, Brown University, Providence, Rhode Island, United States.

Rheumatoid arthritis (RA) is a debilitating autoimmune disease of unknown cause, characterized by infiltration and accumulation of activated immune cells in the synovial joints where cartilage and bone destructions occur. Myeloid-derived suppressor cells (MDSCs) are of myeloid origin and are able to suppress T cell responses. Src homology 2 domain containing inositol polyphosphate 5-phosphatase 1 (SHIP1) was shown to be involved in the regulation of MDSC differentiation. Read More

View Article and Full-Text PDF

Meet your MAKR: the MEMBRANE ASSOCIATED KINASE REGULATOR protein family in the regulation of plant development.

FEBS J 2021 Jul 20. Epub 2021 Jul 20.

Laboratoire Reproduction et Développement des Plantes, Université de Lyon, École normale supérieure de Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, Lyon, 69342, France.

A small family composed of BRI1 KINASE INHIBITOR1 (BKI1) and MEMBRANE ASSOCIATED KINASE REGULATORs (MAKRs) has recently captured the attention of plant biologists, due to their involvement in developmental processes downstream of hormones and Receptor-Like Kinases (RLK) signaling. BKI1/MAKRs are intrinsically disordered proteins (so-called unstructured proteins) and as such lack specific domains. Instead, they are defined by the presence of two conserved linear motifs involved in the interaction with lipids and proteins, respectively. Read More

View Article and Full-Text PDF

Toward Unusual-High Hole Mobility of p-Channel Field-Effect-Transistors.

Small 2021 Jul 19:e2102323. Epub 2021 Jul 19.

School of Physics, School of Microelectronics, State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.

The relative low hole mobility of p-channel building block device challenges the continued miniaturization of modern electronic chips. Metal-semiconductor junction is always an efficient strategy to control the carrier concentration of channel semiconductor, benefiting the carrier mobility regulation of building block device. In this work, complementary metal oxide semiconductor (CMOS)-compatible metals are selected to deposit on the surface of the important p-channel building block of GaSb nanowire field-effect-transistors (NWFETs), demonstrating the efficient strategy of hole mobility enhancement by metal-semiconductor junction. Read More

View Article and Full-Text PDF

Evidence that Increased Cholecystokinin (CCK) in the Periaqueductal Gray (PAG) Facilitates Changes in Resident-Intruder Social Interactions Triggered by Peripheral Nerve Injury.

J Neurochem 2021 Jul 20. Epub 2021 Jul 20.

School of Medical Sciences and the Brain and Mind Centre, The University of Sydney, New South Wales, Australia, 2006.

Individual differences in the effects of a chronic neuropathic injury on social behaviours characterize both the human experience and preclinical animal models. The impacts of these changes to the wellbeing of the individual are often underappreciated. Earlier work from our laboratory using GeneChip® microarrays identified increased cholecystokinin (CCK) gene expression in the periaqueductal gray (PAG) of rats that showed persistent changes in social interactions during a Resident-Intruder encounter following sciatic nerve chronic constriction injury (CCI). Read More

View Article and Full-Text PDF

Commuting to Work: Nucleolar Long Non-Coding RNA Control Ribosome Biogenesis from Near and Far.

Noncoding RNA 2021 Jul 14;7(3). Epub 2021 Jul 14.

Mildred Scheel Early Career Center for Cancer Research (Mildred-Scheel-Nachwuchszentrum, MSNZ), University Hospital Würzburg, Josef-Schneider Str. 2, 97080 Würzburg, Germany.

Gene expression is an essential process for cellular growth, proliferation, and differentiation. The transcription of protein-coding genes and non-coding loci depends on RNA polymerases. Interestingly, numerous loci encode long non-coding (lnc)RNA transcripts that are transcribed by RNA polymerase II (RNAPII) and fine-tune the RNA metabolism. Read More

View Article and Full-Text PDF

Overexpression of the Long Noncoding RNA Inhibits Non-Small Cell Lung Cancer Cell Proliferation and Invasion Through HIF-2a Pathway.

Cancer Biother Radiopharm 2021 Jul 20. Epub 2021 Jul 20.

Department of Radiology, Tuberculosis Hospital of Shaanxi Province, The Fifth People's Hospital of Shaanxi Province, Xi'an, China.

The role and molecular mechanism of long-chain noncoding RNAs (lncRNAs) in lung cancer remain to be elucidated. The aim of this study was to investigate the association between a long coding RNA hypoxia-inducible factor-2α (HIF-2α) promoter upstream transcript () and clinical characteristics of non-small cell lung cancer (NSCLC) and its regulatory role in NSCLC. The correlation between expression and pathological features of NSCLC was analyzed in NSCLC patient samples. Read More

View Article and Full-Text PDF

Rh2-enriched Korean ginseng (Ginseng Rh2+) inhibits tumor growth and development of metastasis of non-small cell lung cancer.

Food Funct 2021 Jul 20. Epub 2021 Jul 20.

Department of Pulmonary Diseases, Tel-Aviv Sourasky Medical Center, Israel.

Background And Objective: While there are multiple studies on the anti-tumoral effects of Panax ginseng as active ingredients (one or more ginsenosides derived from the extract) or as a whole plant extract, there is a lack of studies to assess the effects Panax ginseng's of active ingredients combined with the whole plant extract. Our aim was to study the effect of whole ginseng, enriched in the anti-tumoral Rh2 component and other ginsenosides (Ginseng Rh2+), on the metastatic capacity of non-small cell lung cancer (NSCLC).

Methods: We evaluated the effects of Ginseng Rh2+ on survival, migration and motility, induction of apoptosis, and expression of its apoptosis-related proteins in non-small cell lung cancer (NSCLC) cells in vitro and on primary tumor growth and metastatic capacity in a syngeneic mouse lung cancer model in vivo. Read More

View Article and Full-Text PDF

Knockdown of miR-15b partially reverses the cisplatin resistance of NSCLC through the GSK-3β/MCL-1 pathway.

Adv Clin Exp Med 2021 Jul 20. Epub 2021 Jul 20.

Department of Anesthesia, Nanhua University, Hengyang, China.

Background: Induction of acquired drug resistance occurs frequently with cisplatin-based therapy for non-small cell lung cancer (NSCLC). As recent studies have demonstrated that deregulation of microRNAs (miRNAs) is associated with drug resistance in cancers, correcting the deregulation of miRNAs represents a promising strategy to reverse acquired resistance in NSCLC.

Objectives: This study investigated the functional role of miR-15b in cisplatin resistance in NSCLC. Read More

View Article and Full-Text PDF

Music therapy was noninferior to verbal standard treatment of traumatized refugees in mental health care: Results from a randomized clinical trial.

Eur J Psychotraumatol 2021 6;12(1):1930960. Epub 2021 Jul 6.

Research Unit for Psychotherapy & Psychopathology, Mental Health Service West, Copenhagen University Hospital - Psychiatry Region Zealand, Slagelse, Denmark.

: Many people with refugee backgrounds suffer from trauma-related complex social and psychological problems, and compliance with standard psychological treatment tends to be low. More culturally adaptable treatment options seem to be needed. : We aimed to investigate whether the music therapy method: 'trauma-focused music and imagery' (tr-MI), characterized by a particular focus on arousal and affect regulation, would be equally effective as the standard psychological talk therapies for ameliorating trauma symptoms in Danish refugees. Read More

View Article and Full-Text PDF

LINC01224/ZNF91 Promote Stem Cell-Like Properties and Drive Radioresistance in Non-Small Cell Lung Cancer.

Cancer Manag Res 2021 13;13:5671-5681. Epub 2021 Jul 13.

Departments of Chest Surgery, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, Guangdong, 510095, People's Republic of China.

Background: Radioresistance is the main reason for the failure of radiotherapy in non-small-cell lung cancer (NSCLC); however, the molecular mechanism of radioresistance is still unclear.

Methods: An RNA-Seq assay was used to screen differentially expressed long non-coding RNAs (lncRNAs) and genes in irradiation-resistant NSCLC cells. RT-PCR and Western blotting assays were performed to analyze the expressions of lncRNAs and genes. Read More

View Article and Full-Text PDF

Multi-omics integration of methyltransferase-like protein family reveals clinical outcomes and functional signatures in human cancer.

Sci Rep 2021 Jul 20;11(1):14784. Epub 2021 Jul 20.

Department of Oncology, Wayne State University School of Medicine, Detroit, MI, USA.

Human methyltransferase-like (METTL) proteins transfer methyl groups to nucleic acids, proteins, lipids, and other small molecules, subsequently playing important roles in various cellular processes. In this study, we performed integrated genomic, transcriptomic, proteomic, and clinicopathological analyses of 34 METTLs in a large cohort of primary tumor and cell line data. We identified a subset of METTL genes, notably METTL1, METTL7B, and NTMT1, with high frequencies of genomic amplification and/or up-regulation at both the mRNA and protein levels in a spectrum of human cancers. Read More

View Article and Full-Text PDF

TGF-β1-mediated exosomal lnc-MMP2-2 increases blood-brain barrier permeability via the miRNA-1207-5p/EPB41L5 axis to promote non-small cell lung cancer brain metastasis.

Cell Death Dis 2021 Jul 20;12(8):721. Epub 2021 Jul 20.

School of Clinical Medicine, Chengdu Medical College, Chengdu, Sichuan, PR China.

Brain metastases remain a major problem in patients with advanced non-small cell lung cancer (NSCLC). The permeability of the blood-brain barrier (BBB) is highly increased during lung cancer brain metastasis; however, the underlying mechanism remains largely unknown. We previously found that lnc-MMP2-2 is highly enriched in tumor growth factor (TGF)-β1-mediated exosomes and regulates the migration of lung cancer cells. Read More

View Article and Full-Text PDF

Activation of the intestinal tissue renin--angiotensin system by transient sodium loading in salt-sensitive rats.

J Hypertens 2021 Jul 19. Epub 2021 Jul 19.

Division of Endocrinology, Metabolism, and Nephrology, Department of Internal Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

Background: The renal tissue renin--angiotensin system is known to be activated by salt loading in salt-sensitive rats; however, the response in other organs remains unclear.

Method: Spontaneously hypertensive rats were subjected to normal tap water or transient high-salt-concentration water from 6 to 14 weeks of age and were thereafter given normal tap water. From 18 to 20 weeks of age, rats given water with a high salt concentration were treated with an angiotensin II type 1 receptor blocker, valsartan. Read More

View Article and Full-Text PDF

Non-coding RNAs in the interaction between rice and Meloidogyne graminicola.

BMC Genomics 2021 Jul 20;22(1):560. Epub 2021 Jul 20.

Department of Biotechnology, Ghent University, Ghent, Belgium.

Background: Root knot nematodes (RKN) are plant parasitic nematodes causing major yield losses of widely consumed food crops such as rice (Oryza sativa). Because non-coding RNAs, including small interfering RNAs (siRNA), microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), are key regulators of various plant processes, elucidating their regulation during this interaction may lead to new strategies to improve crop protection. In this study, we aimed to identify and characterize rice siRNAs, miRNAs and lncRNAs responsive to early infection with RKN Meloidogyne graminicola (Mg), based on sequencing of small RNA, degradome and total RNA libraries from rice gall tissues compared with uninfected root tissues. Read More

View Article and Full-Text PDF

Systemic inhibition of PTPN22 augments anticancer immunity.

J Clin Invest 2021 Jul 20. Epub 2021 Jul 20.

Sidney Kimmel Comprehensive Cancer Center, John Hopkins University, Baltimore, United States of America.

Both epidemiologic and cellular studies in the context of autoimmune diseases have established that protein tyrosine phosphatase non-receptor type 22 (PTPN22) is a key regulator of T cell receptor (TCR) signaling. However, its mechanism of action in tumors and its translatability as a target for cancer immunotherapy have not been established. Here we show that a germline variant of PTPN22, rs2476601, portended a lower likelihood of cancer in patients. Read More

View Article and Full-Text PDF

Cellular signaling crosstalk between Wnt signaling and gap junctions inbenzo[a]pyrene toxicity.

Cell Biol Toxicol 2021 Jul 20. Epub 2021 Jul 20.

Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, South Korea.

Gap junctional intercellular communication (GJIC) is considered a key biological mechanism to maintain homeostasis in cell differentiation and growth. In addition, as another major signaling pathway associated with cell proliferation and differentiation, Wnt/β-catenin signaling appears to trigger several cellular responses against injury. The purpose of the present study was to investigate the effects of a known toxic agent, benzo[a]pyrene (BaP), on the regulation and interaction between GJIC and Wnt/β-catenin signaling. Read More

View Article and Full-Text PDF