21,495 results match your criteria regulation ca2+

Indirect evidence that anoxia exposure and cold acclimation alters transarcolemmal Ca flux in the cardiac pacemaker, right atrium and ventricle of the red-eared slider turtle (Trachemys scripta).

Comp Biochem Physiol A Mol Integr Physiol 2021 Jul 29:111043. Epub 2021 Jul 29.

Department of Biological Sciences, University of Alaska Anchorage, Anchorage, AK 99508, United States of America.

We indirectly assessed if altered transarcolemmal Ca flux accompanies the decreased cardiac activity displayed by Trachemys scripta with anoxia exposure and cold acclimation. Turtles were first acclimated to 21 °C or 5 °C and held under normoxic (21 N; 5 N) or anoxic conditions (21A; 5A). We then compared the response of intrinsic heart rate (f) and maximal developed force of spontaneously contracting right atria (F), and maximal developed force of isometrically-contracting ventricular strips (F), to Ni (0. Read More

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Exogenous Ca enhances antioxidant defense in rice to simulated acid rain by regulating ascorbate peroxidase and glutathione reductase.

Planta 2021 Jul 29;254(2):41. Epub 2021 Jul 29.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

Main Conclusion: Exogenous calcium enhances rice tolerance to acid rain stress by regulating isozymes composition and transcriptional expression of ascorbate peroxidase and glutathione reductase. Calcium (Ca) participates in signal transduction in plants under abiotic stress, and addition of Ca is beneficial to alleviate damage of plants caused by acid rain. To clarify the effect of exogenous Ca on tolerance of plants to acid rain stress, we investigated regulation of Ca (5 mM) on activities, isozymes composition and transcriptional expression of ascorbate peroxidase (APX) and glutathione reductase (GR), redox state, and HO concentration and growth in rice leaves and roots under simulated acid rain (SAR) stress. Read More

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2-Aminoethyl diphenylborinate inhibits bleomycin-induced skin and pulmonary fibrosis via interrupting intracellular Ca regulation.

J Dermatol Sci 2021 Jul 13. Epub 2021 Jul 13.

Department of Dermatology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Cosmetic Science, Chang Gung University of Science and Technology, Taoyuan, Taiwan. Electronic address:

Background: Systemic sclerosis (SSc) causes progressive fibrosis of multiple organs with the low efficacy of immunosuppressive therapies. Our previous study indicated the SSc pathological pathways are closely correlated with Ca signals, and blockage of the intracellular Ca elevation facilitates inhibition of SSc pathogenesis.

Objective: Transforming growth factor β (TGF-β)-modulated SMAD signaling is crucial in regulating SSc pathogenesis. Read More

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Precise regulation of presenilin expression is required for sea urchin early development.

J Cell Sci 2021 07 12;134(13). Epub 2021 Jul 12.

Paris-Saclay Institute of Neuroscience, CNRS, Université Paris-Sud, Université Paris-Saclay, 91405 Orsay, France.

Presenilins (PSENs) are widely expressed across eukaryotes. Two PSENs are expressed in humans, where they play a crucial role in Alzheimer's disease (AD). Each PSEN can be part of the γ-secretase complex, which has multiple substrates, including Notch and amyloid-β precursor protein (AβPP) - the source of amyloid-β (Aβ) peptides that compose the senile plaques during AD. Read More

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Substratum stiffness tunes membrane voltage in mammary epithelial cells.

J Cell Sci 2021 07 12;134(13). Epub 2021 Jul 12.

Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.

Membrane voltage (Vm) plays a critical role in the regulation of several cellular behaviors, including proliferation, apoptosis and phenotypic plasticity. Many of these behaviors are affected by the stiffness of the underlying extracellular matrix, but the connections between Vm and the mechanical properties of the microenvironment are unclear. Here, we investigated the relationship between matrix stiffness and Vm by culturing mammary epithelial cells on synthetic substrata, the stiffnesses of which mimicked those of the normal mammary gland and breast tumors. Read More

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Ca transfer via the ER-mitochondria tethering complex in neuronal cells contribute to cadmium-induced autophagy.

Cell Biol Toxicol 2021 Jul 26. Epub 2021 Jul 26.

College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, People's Republic of China.

Mitochondrial-associated endoplasmic reticulum (ER) membranes (MAMs) play a key role in several physiological functions, including calcium ion (Ca) transfer and autophagy; however, the molecular mechanism controlling this interaction in cadmium (Cd)-induced neurotoxicity is unknown. This study shows that Cd induces alterations in MAMs and mitochondrial Ca levels in PC12 cells and primary neurons. Ablation or silencing of mitofusin 2 (Mfn2) in PC12 cells or primary neurons blocks the colocalization of ER and mitochondria while reducing the efficiency of mitochondrial Ca uptake. Read More

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KRAP tethers IP receptors to actin and licenses them to evoke cytosolic Ca signals.

Nat Commun 2021 07 23;12(1):4514. Epub 2021 Jul 23.

Department of Pharmacology, Tennis Court Road, Cambridge, UK.

Regulation of IP receptors (IPRs) by IP and Ca allows regenerative Ca signals, the smallest being Ca puffs, which arise from coordinated openings of a few clustered IPRs. Cells express thousands of mostly mobile IPRs, yet Ca puffs occur at a few immobile IPR clusters. By imaging cells with endogenous IPRs tagged with EGFP, we show that KRas-induced actin-interacting protein (KRAP) tethers IPRs to actin beneath the plasma membrane. Read More

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3D bioprinting of dual-crosslinked nanocellulose hydrogels for tissue engineering applications.

J Mater Chem B 2021 Jul 21. Epub 2021 Jul 21.

School of Chemistry, UNSW Sydney, NSW 2052, Australia.

Hydrogels based on cellulose nanofibrils (CNFs) have been widely used as scaffolds for biomedical applications, however, the poor mechanical properties of CNF hydrogels limit their use as ink for 3D bioprinting in order to generate scaffolds for tissue engineering applications. In this study, a dual crosslinkable hydrogel ink composed of a poly(ethylene glycol) (PEG) star polymer and 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO)-oxidized nanocellulose fibers (CNFs) is presented. As the resulting hydrogel had low structural integrity, at first crosslinking of CNFs was introduced by Ca2+. Read More

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The CaCA superfamily genes in Saccharum: comparative analysis and their functional implications in response to biotic and abiotic stress.

BMC Genomics 2021 Jul 18;22(1):549. Epub 2021 Jul 18.

Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, China.

Background: In plants, Calcium (Ca) acts as a universal messenger in various signal transduction pathways, including responses to biotic and abiotic stresses and regulation of cellular and developmental processes. The Ca/cation antiporter (CaCA) superfamily proteins play vital roles in the transport of Ca and/or other cations. However, the characteristics of these superfamily members in Saccharum and their evolutionary and functional implications have remained unclear. Read More

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Natriuretic peptide receptor B maintains heart rate and sinoatrial node function via cyclic GMP-mediated signaling.

Cardiovasc Res 2021 Jul 17. Epub 2021 Jul 17.

Libin Cardiovascular Institute, Department of Cardiac Sciences, Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.

Aims: Heart rate (HR) is a critical indicator of cardiac performance that is determined by sinoatrial node (SAN) function and regulation. Natriuretic peptides, including C-type NP (CNP) have been shown to modulate ion channel function in the SAN when applied exogenously. CNP is the only NP that acts as a ligand for natriuretic peptide receptor-B (NPR-B). Read More

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Mandibular muscle troponin of the Florida carpenter ant Camponotus floridanus: extending our insights into invertebrate Ca regulation.

J Muscle Res Cell Motil 2021 Jul 13. Epub 2021 Jul 13.

Department of Biological Science, Florida State University, Tallahassee, FL, 32306, USA.

Ants use their mandibles for a variety of functions and behaviors. We investigated mandibular muscle structure and function from major workers of the Florida carpenter ant Camponotus floridanus: force-pCa relation and velocity of unloaded shortening of single, permeabilized fibres, primary sequences of troponin subunits (TnC, TnI and TnT) from a mandibular muscle cDNA library, and muscle fibre ultrastructure. From the mechanical measurements, we found Ca-sensitivity of isometric force was markedly shifted rightward compared with vertebrate striated muscle. Read More

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TRP channels in health and disease at a glance.

J Cell Sci 2021 Jul 13;134(13). Epub 2021 Jul 13.

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.

The transient receptor potential (TRP) channel superfamily consists of a large group of non-selective cation channels that serve as cellular sensors for a wide spectrum of physical and environmental stimuli. The 28 mammalian TRPs, categorized into six subfamilies, including TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPA (ankyrin), TRPML (mucolipin) and TRPP (polycystin), are widely expressed in different cells and tissues. TRPs exhibit a variety of unique features that not only distinguish them from other superfamilies of ion channels, but also confer diverse physiological functions. Read More

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Synaptic contributions to cochlear outer hair cell Ca dynamics.

J Neurosci 2021 Jul 12. Epub 2021 Jul 12.

Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI), C. A. Buenos Aires (1428) Argentina

For normal cochlear function, outer hair cells (OHCs) require a precise control of intracellular Ca levels. In the absence of regulatory elements such as proteinaceous buffers or extrusion pumps, OHCs degenerate, leading to profound hearing impairment. Influx of Ca occurs both at the stereocilia tips and the basolateral membrane. Read More

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Mitochondrial Ca and cell cycle regulation.

Haixin Zhao Xin Pan

Int Rev Cell Mol Biol 2021 9;362:171-207. Epub 2021 Apr 9.

State Key Laboratory of Proteomics, Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China. Electronic address:

It has been demonstrated for more than 40 years that intracellular calcium (Ca) controls a variety of cellular functions, including mitochondrial metabolism and cell proliferation. Cytosolic Ca fluctuation during key stages of the cell cycle can lead to mitochondrial Ca uptake and subsequent activation of mitochondrial oxidative phosphorylation and a range of signaling. However, the relationship between mitochondrial Ca and cell cycle progression has long been neglected because the molecule responsible for Ca uptake has been unknown. Read More

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Analysis of the effects of different nitrogen sources and calcium on the production of amphotericin by Streptomyces nodosus based on comparative transcriptome.

Biotechnol Appl Biochem 2021 Jul 12. Epub 2021 Jul 12.

The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, China.

Streptomyces nodosus is known as the main manufacturer of amphotericin B (AmB), which is an effective antifungal drug. It is verified that the optimization of fermentation conditions and key growth factors have a great impact on the yield of AmB. The AmB production of S. Read More

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Separation of presynaptic Ca2 and Ca1 channel function in synaptic vesicle exo- and endocytosis by the membrane anchored Ca pump PMCA.

Proc Natl Acad Sci U S A 2021 Jul;118(28)

Institute of Developmental Biology and Neurobiology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany;

Synaptic vesicle (SV) release, recycling, and plastic changes of release probability co-occur side by side within nerve terminals and rely on local Ca signals with different temporal and spatial profiles. The mechanisms that guarantee separate regulation of these vital presynaptic functions during action potential (AP)-triggered presynaptic Ca entry remain unclear. Combining genetics with electrophysiology and imaging reveals the localization of two different voltage-gated calcium channels at the presynaptic terminals of glutamatergic neuromuscular synapses (the Ca2 homolog, Dmca1A or cacophony, and the Ca1 homolog, Dmca1D) but with spatial and functional separation. Read More

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Calmodulin complexes with brain and muscle creatine kinase peptides.

Curr Res Struct Biol 2021 19;3:121-132. Epub 2021 May 19.

Department of Biochemistry and Structural Biology, Chemical Center, PO Box 124, SE-221 00, Lund, Sweden.

Calmodulin (CaM) is a ubiquitous Ca sensing protein that binds to and modulates numerous target proteins and enzymes during cellular signaling processes. A large number of CaM-target complexes have been identified and structurally characterized, revealing a wide diversity of CaM-binding modes. A newly identified target is creatine kinase (CK), a central enzyme in cellular energy homeostasis. Read More

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A Novel Conceptual Model for the Dual Role of FF-ATP Synthase in Cell Life and Cell Death.

Sunil Nath

Biomol Concepts 2020 Aug 22;11(1):143-152. Epub 2020 Aug 22.

Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi110016, India.

The mitochondrial permeability transition (MPT) has been one of the longstanding enigmas in biology. Its cause is currently at the center of an extensive scientific debate, and several hypotheses on its molecular nature have been put forward. The present view holds that the transition arises from the opening of a high-conductance channel in the energy-transducing membrane, the permeability transition pore (PTP), also called the mitochondrial megachannel or the multiconductance channel (MMC). Read More

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Rotavirus research: 2014-2020.

Virus Res 2021 Jul 2:198499. Epub 2021 Jul 2.

Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK. Electronic address:

Rotaviruses are major causes of acute gastroenteritis in infants and young children worldwide and also cause disease in the young of many other mammalian and of avian species. During the recent 5-6 years rotavirus research has benefitted in a major way from the establishment of plasmid only-based reverse genetics systems, the creation of human and other mammalian intestinal enteroids, and from the wide application of structural biology (cryo-electron microscopy, cryo-EM tomography) and complementary biophysical approaches. All of these have permitted to gain new insights into structure-function relationships of rotaviruses and their interactions with the host. Read More

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IRE1-mTOR-PERK Axis Coordinates Autophagy and ER Stress-Apoptosis Induced by P2X7-Mediated Ca Influx in Osteoarthritis.

Front Cell Dev Biol 2021 17;9:695041. Epub 2021 Jun 17.

Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, China.

Moderate-intensity exercise can help delay the development of osteoarthritis (OA). Previous studies have shown that the purinergic receptor P2X ligand gated ion channel 7 (P2X7) is involved in OA development and progression. To investigate the effect of exercise on P2X7 activation and downstream signaling in OA, we used the anterior cruciate ligament transection (ACLT)-induced OA rat model and primary chondrocyte culture system. Read More

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A store-operated Ca-entry in Trypanosoma equiperdum: Physiological evidences of its presence.

Mol Biochem Parasitol 2021 07 1;244:111394. Epub 2021 Jul 1.

Centro de Estudios Biomédicos y Veterinarios, Instituto de Estudios Científicos y Tecnológicos (IDECYT), Universidad Nacional Experimental Simón Rodríguez, Caracas, Venezuela. Electronic address:

The Trypanosomatidae family encompasses many unicellular organisms responsible of several tropical diseases that affect humans and animals. Livestock tripanosomosis caused by Trypanosoma brucei brucei (T. brucei), Trypanosoma equiperdum (T. Read More

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Prokineticin 1-prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development.

Sci Rep 2021 Jul 2;11(1):13715. Epub 2021 Jul 2.

Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.

Successful pregnancy establishment in mammals depends on proper embryo-maternal communication. Prokineticin 1 (PROK1) is a secretory protein that exerts pleiotropic functions in various tissues. Despite the studies that have primarily been performed with human cell lines and mice, the function of PROK1 in trophoblasts has still not been fully elucidated. Read More

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[Single-molecule electrophoresis: renewed understanding of nanopore electrochemistry].

Se Pu 2020 Sep;38(9):993-998

School of Chemistry and Molecule Engineering, East China University of Science and Technology, Shanghai 200237, China.

This study aims to understand nanopore technology from the standpoint of capillary electrophoresis separation. The nanopore electrochemical measurements could be regarded as "single molecule electrophoresis". Similar to the case of capillary electrophoresis, the single target molecules migrate inside a nanopore under an external electric field. Read More

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September 2020

Uroguanylin increases Ca2+ concentration in astrocytes via guanylate cyclase C-independent signaling pathway.

Croat Med J 2021 Jun;62(3):250-263

Aleksandra Dugandžić (née Sinđić), Department of Physiology, Croatian Institute for Brain Research, Zagreb University School of Medicine, Šalata 3, 10000 Zagreb, Croatia,

Aim: To investigate the cyclic guanosine monophosphate (cGMP)/guanylate cyclase C (GC-C)-independent signaling pathway in astrocytes, which are a suitable model due to their lack of GC-C expression.

Methods: Patch clamp was performed and intracellular Ca2+ concentrations and pH were measured in primary astrocyte cultures and brain slices of wild type (WT) and GC-C knockout (KO) mice. The function of GC-C-independent signaling pathway in the cerebellum was determined by behavior tests in uroguanylin (UGN) KO and GC-C KO mice. Read More

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Interventricular Differences of Signaling Pathways-Mediated Regulation of Cardiomyocyte Function in Response to High Oxidative Stress in the Post-Ischemic Failing Rat Heart.

Antioxidants (Basel) 2021 Jun 16;10(6). Epub 2021 Jun 16.

Institut für Forschung und Lehre (IFL) Molecular and Experimental Cardiology, St. Josef-Hospital, Ruhr University Bochum, 44801 Bochum, Germany.

Standard heart failure (HF) therapies have failed to improve cardiac function or survival in HF patients with right ventricular (RV) dysfunction suggesting a divergence in the molecular mechanisms of RV vs. left ventricular (LV) failure. Here we aimed to investigate interventricular differences in sarcomeric regulation and function in experimental myocardial infarction (MI)-induced HF with reduced LV ejection fraction (HFrEF). Read More

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Sigma-1 Receptor Promotes Mitochondrial Bioenergetics by Orchestrating ER Ca Leak during Early ER Stress.

Metabolites 2021 Jun 26;11(7). Epub 2021 Jun 26.

Molecular Biology and Biochemistry, Gottfried Schatz Research Center, Medical University of Graz, Neue Stiftingtalstraße 6/6, 8010 Graz, Austria.

The endoplasmic reticulum (ER) is a complex, multifunctional organelle of eukaryotic cells and responsible for the trafficking and processing of nearly 30% of all human proteins. Any disturbance to these processes can cause ER stress, which initiates an adaptive mechanism called unfolded protein response (UPR) to restore ER functions and homeostasis. Mitochondrial ATP production is necessary to meet the high energy demand of the UPR, while the molecular mechanisms of ER to mitochondria crosstalk under such stress conditions remain mainly enigmatic. Read More

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Calcium Regulation on the Atrial Regional Difference of Collagen Production Activity in Atrial Fibrogenesis.

Biomedicines 2021 Jun 17;9(6). Epub 2021 Jun 17.

Division of Cardiology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.

Background: Atrial fibrosis plays an important role in the genesis of heart failure and atrial fibrillation. The left atrium (LA) exhibits a higher level of fibrosis than the right atrium (RA) in heart failure and atrial arrhythmia. However, the mechanism for the high fibrogenic potential of the LA fibroblasts remains unclear. Read More

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RGS14 Regulation of Post-Synaptic Signaling and Spine Plasticity in Brain.

Int J Mol Sci 2021 Jun 25;22(13). Epub 2021 Jun 25.

Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.

The regulator of G-protein signaling 14 (RGS14) is a multifunctional signaling protein that regulates post synaptic plasticity in neurons. RGS14 is expressed in the brain regions essential for learning, memory, emotion, and stimulus-induced behaviors, including the basal ganglia, limbic system, and cortex. Behaviorally, RGS14 regulates spatial and object memory, female-specific responses to cued fear conditioning, and environmental- and psychostimulant-induced locomotion. Read More

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T-Type Ca Enhancer SAK3 Activates CaMKII and Proteasome Activities in Lewy Body Dementia Mice Model.

Int J Mol Sci 2021 Jun 8;22(12). Epub 2021 Jun 8.

Departments of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.

Lewy bodies are pathological characteristics of Lewy body dementia (LBD) and are composed of α-synuclein (α-Syn), which is mostly degraded via the ubiquitin-proteasome system. More importantly, 26S proteasomal activity decreases in the brain of LBD patients. We recently introduced a T-type calcium channel enhancer SAK3 (ethyl-8-methyl-2,4-dioxo-2-(piperidin-1-yl)- 2H-spiro[cyclopentane-1,3-imidazo [1,2-a]pyridin]-2-ene-3-carboxylate) for Alzheimer's disease therapeutics. Read More

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Gender-Dependent Phenotype in Polycystic Kidney Disease Is Determined by Differential Intracellular Ca Signals.

Int J Mol Sci 2021 Jun 2;22(11). Epub 2021 Jun 2.

Physiological Institute, University of Regensburg, University Street 31, D-93053 Regensburg, Germany.

Autosomal dominant polycystic kidney disease (ADPKD) is caused by loss of function of PKD1 (polycystin 1) or PKD2 (polycystin 2). The Ca-activated Cl channel TMEM16A has a central role in ADPKD. Expression and function of TMEM16A is upregulated in ADPKD which causes enhanced intracellular Ca signaling, cell proliferation, and ion secretion. Read More

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