1,408 results match your criteria regulating transgene

Roles of autophagy in orthodontic tooth movement.

Am J Orthod Dentofacial Orthop 2021 Mar 23. Epub 2021 Mar 23.

Department of Orthodontics, School of Dentistry, University of North Carolina, Chapel Hill, Nc. Electronic address:

Introduction: Orthodontic tooth movement (OTM) relies on efficient remodeling of alveolar bone. While a well-controlled inflammatory response is essential during OTM, the mechanism regulating inflammation is unknown. Autophagy, a conserved catabolic pathway, has been shown to protect cells from excess inflammation in disease states. Read More

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Human progranulin-expressing mice as a novel tool for the development of progranulin-modulating therapeutics.

Neurobiol Dis 2021 Jun 24;153:105314. Epub 2021 Feb 24.

Centre for Molecular Medicine & Therapeutics, Department of Medical Genetics, University of British Columbia, B.C. Children's Hospital, 950 West 28(th) Avenue, Vancouver, BC V5Z 4H4, Canada; Division of Neurology, Department of Medicine, University of British Columbia Hospital, S 192 - 2211 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada; Center for Brain Health, University of British Columbia, Vancouver, BC V6T 1Z3, Canada. Electronic address:

The granulin protein (also known as, and hereafter referred to as, progranulin) is a secreted glycoprotein that contributes to overall brain health. Heterozygous loss-of-function mutations in the gene encoding the progranulin protein (Granulin Precursor, GRN) are a common cause of familial frontotemporal dementia (FTD). Gene therapy approaches that aim to increase progranulin expression from a single wild-type allele, an area of active investigation for the potential treatment of GRN-dependent FTD, will benefit from the availability of a mouse model that expresses a genomic copy of the human GRN gene. Read More

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Arabidopsis Ca2+-ATPases 1, 2, and 7 in the endoplasmic reticulum contribute to growth and pollen fitness.

Plant Physiol 2021 Jan 28. Epub 2021 Jan 28.

Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Nevada 89557, USA.

Generating cellular Ca2+ signals requires coordinated transport activities from both Ca2+ influx and efflux pathways. In Arabidopsis (Arabidopsis thaliana), multiple efflux pathways exist, some of which involve Ca2+-pumps belonging to the Autoinhibited Ca2+-ATPase (ACA) family. Here, we show that ACA1, 2, and 7 localize to the endoplasmic reticulum (ER) and are important for plant growth and pollen fertility. Read More

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January 2021

Molecular characterization of SlATG18f in response to Tomato leaf curl New Delhi virus infection in tomato and development of a CAPS marker for leaf curl disease tolerance.

Theor Appl Genet 2021 Feb 7. Epub 2021 Feb 7.

National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India.

Key Message: Analysis of autophagy-related genes in tomato shows the involvement of SlATG18f in leaf curl disease tolerance and a CAPS marker developed from this gene demonstrates its usefulness in marker-assisted selection. Autophagy is a highly conserved catabolic process regulating cellular homeostasis and adaptation to different biotic and abiotic stress. Several autophagy-related proteins (ATGs) are reported to be involved in autophagic processes, and considering their importance in regulating growth and stress adaptation, these proteins have been identified and characterized in several plant species. Read More

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February 2021

Tyramine synthesis, vesicular packaging, and the SNARE complex function coordinately in astrocytes to regulate Drosophila alcohol sedation.

Addict Biol 2021 Feb 3:e13019. Epub 2021 Feb 3.

Neuroscience Graduate Program, Virginia Commonwealth University, Richmond, Virginia, USA.

Identifying mechanisms underlying alcohol-related behaviors could provide important insights regarding the etiology of alcohol use disorder. To date, most genetic studies on alcohol-related behavior in model organisms have focused on neurons, leaving the causal roles of glial mechanisms less comprehensively investigated. Here, we report our studies on the role of Tyrosine decarboxylase 2 (Tdc2), which converts tyrosine to the catecholamine tyramine, in glial cells in Drosophila alcohol sedation. Read More

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February 2021

Genome-wide association analysis reveals genetic variations and candidate genes associated with salt tolerance related traits in Gossypium hirsutum.

BMC Genomics 2021 Jan 6;22(1):26. Epub 2021 Jan 6.

Provincial Key Laboratory of Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing, 210014, China.

Background: Cotton is more resistant to salt and drought stresses as compared to other field crops, which makes itself as a pioneer industrial crop in saline-alkali lands. However, abiotic stresses still negatively affect its growth and development significantly. It is therefore important to breed salt tolerance varieties which can help accelerate the improvement of cotton production. Read More

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January 2021

Overexpression of an Antisense RNA of Maize Receptor-Like Kinase Gene Enlarges the Organ and Seed Size of Transgenic Plants.

Front Plant Sci 2020 11;11:579120. Epub 2020 Nov 11.

Maize Research Institute, Shandong Academy of Agricultural Sciences/National Engineering Laboratory of Wheat and Maize/Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-huai River Plain, Ministry of Agriculture, Jinan, China.

Leucine-rich repeat (LRR)-receptor-like protein kinases (LRR-RLKs) play vital roles in plant growth, development, and responses to environmental stresses. In this study, a new LRR-RLK gene, , was isolated from maize, and its function within plant development was investigated through ectopic expression in . The spatial expression pattern analysis reveals that is highly expressed in embryos prior to programmed cell death (PCD) of starchy endosperm tissues, and its encoded protein has been localized to both plasm and nuclear membranes subcellularly. Read More

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November 2020

Enhanced glutathione content improves lateral root development and grain yield in rice plants.

Plant Mol Biol 2021 Mar 18;105(4-5):365-383. Epub 2020 Nov 18.

Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.

Key Message: Enhanced glutathione content improves lateral root development by positively regulating the transcripts of root development genes responsive to glutathione treatment, thereby increasing the overall productivity of rice plants. Glutathione is primarily known as a cellular antioxidant molecule, but its role in lateral root development in rice plants has not been elucidated. Here, we have investigated its role in lateral root development of rice Oryza sativa L. Read More

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Transient Nodal Signaling in Left Precursors Coordinates Opposed Asymmetries Shaping the Heart Loop.

Dev Cell 2020 11 9;55(4):413-431.e6. Epub 2020 Nov 9.

Université de Paris, Imagine - Institut Pasteur, Unit of Heart Morphogenesis, INSERM UMR1163, 75015 Paris, France. Electronic address:

The secreted factor Nodal, known as a major left determinant, is associated with severe heart defects. Yet, it has been unclear how it regulates asymmetric morphogenesis such as heart looping, which align cardiac chambers to establish the double blood circulation. Here, we report that Nodal is transiently active in precursors of the mouse heart tube poles, before looping. Read More

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November 2020

Functional Analysis of the Gene in the Tetraploid Switchgrass (.) by CRISPR/Cas9-Directed Mutagenesis.

Front Plant Sci 2020 23;11:572193. Epub 2020 Sep 23.

Interdepartmental Program in Plant Biology, Iowa State University, Ames, IA, United States.

Tillering is an important biomass yield component trait in switchgrass (.). ()/ () gene is a known regulator for tillering/branching in several plant species; however, its role on tillering in switchgrass remains unknown. Read More

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September 2020

An update and perspectives on the use of promoters in plant genetic engineering.

J Biosci 2020 ;45

International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502 324, India.

Genetically engineered plants have varied applications in agriculture for enhancing the values of food and feed. Genetic engineering aims to introduce selected genetic regions with desirable traits into target plants for both spatial and temporal expressions. Promoters are the key elements responsible for regulating gene expressions by modulating the transcription factors (TFs) through recognition of RNA polymerases. Read More

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January 2020

Fatty acid-binding protein 3 controls contact hypersensitivity through regulating skin dermal Vγ4 γ/δ T cell in a murine model.

Allergy 2020 Oct 22. Epub 2020 Oct 22.

Department of Organ Anatomy, Tohoku University Graduate School of Medicine, Sendai, Japan.

Background: Fatty acid-binding protein 3 (FABP3) is a cytosolic carrier protein of polyunsaturated fatty acids (PUFAs) and regulates cellular metabolism. However, the physiological functions of FABP3 in immune cells and how FABP3 regulates inflammatory responses remain unclear.

Methods: Contact hypersensitivity (CHS) induced by 2,4-dinitrofluorobenzene (DNFB) and fluorescein isothiocyanate was applied to the skin wild-type and Fabp3 mice. Read More

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October 2020

Regional Differences in Penetration of the Protein Stabilizer Trimethoprim (TMP) in the Rat Central Nervous System.

Front Mol Neurosci 2020 3;13:167. Epub 2020 Sep 3.

Department of Health Sciences and Technology, Brain Research Institute, University of Zurich, ETH Zürich, Zurich, Switzerland.

Regulating gene expression at the protein level is becoming increasingly important for answering basic questions in neurobiology. Several techniques using destabilizing domains (DD) on transgenes, which can be activated or deactivated by specific drugs, have been developed to achieve this goal. A DD from bacterial dihydrofolate reductase bound and stabilized by trimethoprim (TMP) represents such a tool. Read More

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September 2020

A dual role for Tbx1 in cardiac lymphangiogenesis through genetic interaction with Vegfr3.

FASEB J 2020 11 20;34(11):15062-15079. Epub 2020 Sep 20.

Department of Chemistry and Biology, University of Salerno, Fisciano, Italy.

The transcription factor TBX1 is the major gene implicated in 22q11.2 deletion syndrome (22q11.2DS). Read More

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November 2020

Activity of transgene-produced B-domain-deleted factor VIII in human plasma following AAV5 gene therapy.

Blood 2020 11;136(22):2524-2534

BioMarin Pharmaceutical Inc, Novato, CA.

Adeno-associated virus (AAV)-based gene therapies can restore endogenous factor VIII (FVIII) expression in hemophilia A (HA). AAV vectors typically use a B-domain-deleted FVIII transgene, such as human FVIII-SQ in valoctocogene roxaparvovec (AAV5-FVIII-SQ). Surprisingly, the activity of transgene-produced FVIII-SQ was between 1. Read More

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November 2020

Intracellular O-linked glycosylation directly regulates cardiomyocyte L-type Ca channel activity and excitation-contraction coupling.

Basic Res Cardiol 2020 09 10;115(6):59. Epub 2020 Sep 10.

Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of Medicine and College of Science and Mathematics, Wright State University, 143 Biological Sciences II, 3640 Colonel Glenn Hwy, Dayton, OH, 45435, USA.

Cardiomyocyte L-type Ca channels (Cas) are targets of signaling pathways that modulate channel activity in response to physiologic stimuli. Ca regulation is typically transient and beneficial but chronic stimulation can become pathologic; therefore, gaining a more complete understanding of Ca regulation is of critical importance. Intracellular O-linked glycosylation (O-GlcNAcylation), which is the result of two enzymes that dynamically add and remove single N-acetylglucosamines to and from intracellular serine/threonine residues (OGT and OGA respectively), has proven to be an increasingly important post-translational modification that contributes to the regulation of many physiologic processes. Read More

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September 2020

Peroxiredoxin 6 mediates protective function of astrocytes in Aβ proteostasis.

Mol Neurodegener 2020 09 9;15(1):50. Epub 2020 Sep 9.

Department of Neurology, New York University Grossman School of Medicine, 550 First Avenue, Science Building, Room 10-07, New York, NY, 10016, USA.

Background: Disruption of β-amyloid (Aβ) homeostasis is the initial culprit in Alzheimer's disease (AD) pathogenesis. Astrocytes respond to emerging Aβ plaques by altering their phenotype and function, yet molecular mechanisms governing astrocytic response and their precise role in countering Aβ deposition remain ill-defined. Peroxiredoxin (PRDX) 6 is an enzymatic protein with independent glutathione peroxidase (Gpx) and phospholipase A2 (PLA) activities involved in repair of oxidatively damaged cell membrane lipids and cellular signaling. Read More

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September 2020

CRISPR/Cas9 Directed Mutagenesis of in High Yielding Basmati Rice ( L.) Line and Comparative Proteome Profiling of Unveiled Changes Triggered by Mutations.

Int J Mol Sci 2020 Aug 26;21(17). Epub 2020 Aug 26.

College of Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, China.

In rice, semi-dwarfism is among the most required characteristics, as it facilitates better yields and offers lodging resistance. Here, semi-dwarf rice lines lacking any residual transgene-DNA and off-target effects were generated through CRISPR/Cas9-guided mutagenesis of the gene in a high yielding Basmati rice line, and the isobaric tags for relative and absolute quantification (iTRAQ) strategy was utilized to elucidate the proteomic changes in mutants. The results indicated the reduced gibberellins (GA and GA) levels, plant height (28. Read More

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Intramuscular Delivery of Gene Therapy for Targeting the Nervous System.

Front Mol Neurosci 2020 17;13:129. Epub 2020 Jul 17.

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology, University College London, London, United Kingdom.

Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease. This is particularly enticing for neuronal conditions, as the nervous system is renowned for its intransigence to therapeutic targeting. Administration of gene therapy viruses into skeletal muscle, where distal terminals of motor and sensory neurons reside, has been shown to result in extensive transduction of cells within the spinal cord, brainstem, and sensory ganglia. Read More

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SlEAD1, an EAR motif-containing ABA down-regulated novel transcription repressor regulates ABA response in tomato.

GM Crops Food 2020 Oct;11(4):275-289

Laboratory of Plant Molecular Genetics & Crop Gene Editing, School of Life Sciences, Linyi University , Linyi, China.

EAR motif-containing proteins are able to repress gene expression, therefore play important roles in regulating plants growth and development, plant response to environmental stimuli, as well as plant hormone signal transduction. ABA is a plant hormone that regulates abiotic stress tolerance in plants via signal transduction. ABA signaling via the PYR1/PYLs/RCARs receptors, the PP2Cs phosphatases, and SnRK2s protein kinases activates the ABF/AREB/ABI5-type bZIP transcription factors, resulting in the activation/repression of ABA response genes. Read More

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October 2020

Mutations in Ciliary Trafficking Genes affect Sonic Hedgehog-dependent Neural Tube Patterning Differentially along the Anterior-Posterior Axis.

Neuroscience 2020 12 16;450:3-14. Epub 2020 Jul 16.

Vertebrate Developmental Biology Program, Department of Pediatrics, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, United States. Electronic address:

Cell specification in the ventral spinal cord is a well-studied model system to understand how tissue pattern develops in response to a morphogen gradient. Ventral cell types including motor neurons (MNs) are induced in the neural tube in response to graded Sonic Hedgehog (Shh) signaling. We performed a forward genetic screen in the mouse that incorporated a GFP-expressing transgene to visualize MNs to identify genes regulating ventral patterning. Read More

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December 2020

Generation of transgenic mice that conditionally express microRNA miR-145.

Genesis 2020 09 10;58(9):e23385. Epub 2020 Jul 10.

Center for Cardiovascular Research and The Heart Center, Nationwide Children's Hospital, Columbus, Ohio, USA.

MicroRNAs are modulators of cellular phenotypes and their functions contribute to development, homeostasis, and disease. miR-145 is a conserved microRNA that has been implicated in regulating an array of phenotypes. These include supporting smooth muscle differentiation, repression of stem cell pluripotency, and inhibition of tumor growth and metastasis. Read More

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September 2020

Genome-wide analysis of brassinosteroid responsive small RNAs in Arabidopsis thaliana.

Genes Genomics 2020 08 9;42(8):957-969. Epub 2020 Jul 9.

Department of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon, 34134, Korea.

Background: Brassinosteroids (BRs) are a class of phytohormones with important roles in regulating physiological and developmental processes. Small RNAs, including small interfering RNAs and microRNAs (miRNAs), are non-protein coding RNAs that regulate gene expression at the transcriptional and post-transcriptional levels. However, the roles of small RNAs in BR response have not been studied well. Read More

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Increased mAb production in amplified CHO cell lines is associated with increased interaction of CREB1 with transgene promoter.

Curr Res Biotechnol 2019 Nov 5;1:49-57. Epub 2019 Oct 5.

College of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, NY, USA.

Most therapeutic monoclonal antibodies in biopharmaceutical processes are produced in Chinese hamster ovary (CHO) cells. Technological advances have rendered the selection procedure for higher producers a robust protocol. However, information on molecular mechanisms that impart the property of hyper-productivity in the final selected clones is currently lacking. Read More

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November 2019

Generation of three induced pluripotent stem cell (iPSC) lines from a patient with developmental epileptic encephalopathy due to the pathogenic KCNA2 variant c.869T>G; p.Leu290Arg (NUIGi052-A, NUIGi052-B, NUIGi052-C).

Stem Cell Res 2020 07 1;46:101853. Epub 2020 Jun 1.

Department of Paediatrics, School of Medicine, National University of Ireland Galway, Ireland; Regenerative Medicine Institute, School of Medicine, National University of Ireland Galway, Ireland. Electronic address:

De novo pathogenic variants in KCNA2 are implicated in causing a spectrum of human neurological disorders, in particular developmental and epileptic encephalopathies. KCNA2 encodes the voltage-gated delayed rectifier potassium channel K1.2, which is vital in regulating neuronal membrane potential and repolarization. Read More

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Profiling and quantification of pluripotency reprogramming reveal that WNT pathways and cell morphology have to be reprogramed extensively.

Heliyon 2020 May 27;6(5):e04035. Epub 2020 May 27.

Heflin Center for Genomic Sciences, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.

Pluripotent state can be established via reprogramming of somatic nuclei by factors within an oocyte or by ectopic expression of a few transgenes. Considered as being extensive and intensive, the full complement of genes to be reprogrammed, however, has never been defined, nor has the degree of reprogramming been determined quantitatively. Here, we propose a new concept of reprogramome, which is defined as the full complement of genes to be reprogrammed to the expression levels found in pluripotent stem cells (PSCs). Read More

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Snail induces epithelial cell extrusion by regulating RhoA contractile signalling and cell-matrix adhesion.

J Cell Sci 2020 07 6;133(13). Epub 2020 Jul 6.

Division of Cell and Developmental Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia

Cell extrusion is a morphogenetic process that is implicated in epithelial homeostasis and elicited by stimuli ranging from apoptosis to oncogenic transformation. To explore whether the morphogenetic transcription factor Snail (SNAI1) induces extrusion, we inducibly expressed a stabilized Snail transgene in confluent MCF-7 monolayers. When expressed in small clusters (less than three cells) within otherwise wild-type confluent monolayers, Snail expression induced apical cell extrusion. Read More

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Heat stress inducible cytoplasmic isoform of ClpB1 from Z. nummularia exhibits enhanced thermotolerance in transgenic tobacco.

Mol Biol Rep 2020 May 4;47(5):3821-3831. Epub 2020 May 4.

ICAR-National Institute for Plant Biotechnology, New Delhi, India.

Previously, we isolated CDS of Ziziphus nummularia isoform ZnJClpB1-C from heat stress-tolerant genotype Jaisalmer. To further functionally validate ZnJClpB1-C assumed function in tobacco and to generate novel germplasm for heat stress tolerance, this gene was transformed in the Nicotiana tabacum. ClpB proteins are the major key player required for basal and induced heat stress tolerance in plant cells under heat stress. Read More

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The first quarter of the C-terminal domain of Abelson regulates the WAVE regulatory complex and Enabled in axon guidance.

Neural Dev 2020 05 2;15(1). Epub 2020 May 2.

Department of Biological Sciences, Wayne State University, Detroit, MI, 48202, USA.

Background: Abelson tyrosine kinase (Abl) plays a key role in axon guidance in linking guidance receptors to actin dynamics. The long C-terminal domain (CTD) of Drosophila Abl is important for this role, and previous work identified the 'first quarter' (1Q) of the CTD as essential. Here, we link the physical interactions of 1Q binding partners to Abl's function in axon guidance. Read More

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Inhibition of cGAS-Mediated Interferon Response Facilitates Transgene Expression.

iScience 2020 Apr 31;23(4):101026. Epub 2020 Mar 31.

Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University Qishan Campus, College Town, Fuzhou, Fujian Province 350117, China. Electronic address:

DNA transfection is often the bottleneck of research and gene therapy practices. To explore the mechanism regulating transgene expression, we investigated the role of the cGAS-STING signaling pathway, which induces type-I interferons in response to DNA. We confirmed that deletion of cGAS enhances transgene expression at the protein level by ~2- to 3-fold. Read More

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