9,608 results match your criteria region leucine


HOPX injury-resistant intestinal stem cells drive epithelial recovery after severe intestinal ischemia.

Am J Physiol Gastrointest Liver Physiol 2021 Sep 22. Epub 2021 Sep 22.

College of Veterinary Medicine, grid.40803.3fNorth Carolina State University, Raleigh, NC, United States.

Intestinal ischemia is a life-threatening emergency with mortality rates of 50-80% due to epithelial cell death and resultant barrier loss.Loss of the epithelial barrier occurs in conditions including intestinal volvulus and neonatal necrotizing enterocolitis.Survival depends on effective epithelial repair; crypt-based intestinal epithelial stem cells (ISCs) are the source of epithelial renewal in homeostasis and after injury. Read More

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September 2021

High-resolution mapping of the quantitative trait locus (QTLs) conferring resistance to false smut disease in rice.

J Appl Genet 2021 Sep 17. Epub 2021 Sep 17.

School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.

Rice false smut (RFS), an emerging major fungal disease worldwide caused by Ustilaginoidea virens, affects rice grain quality and yield. RFS cause 2.8-49% global yield loss depending upon disease severity and cultivars. Read More

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September 2021

Feeding containing the aerial part of promotes the growth and nutritive value of rabbit fish .

Food Sci Nutr 2021 Sep 28;9(9):4827-4838. Epub 2021 Jul 28.

Shenzhen Key Laboratory of Edible and Medicinal Bioresources HKUST Shenzhen Research Institute Shenzhen China.

The root of (Scutellaria Radix) has been used as herbal medicine for years, while its stem and leaf (aerial part) are considered as waste. The water extract from the aerial part of (named as SBA) being included in the feeding of (grey rabbit fish) has been shown to replace antibiotics in aquaculture with excellent outcome. To strengthen the usage of SBA in fish feeding, the total fish output and its nutritive value were determined here. Read More

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September 2021

Impact of Type II LRRK2 inhibitors on signalling and mitophagy.

Biochem J 2021 Sep 13. Epub 2021 Sep 13.

University of Dundee, DUNDEE, United Kingdom.

Much effort has been devoted to the development of selective inhibitors of the LRRK2 as a potential treatment for LRRK2 driven Parkinson's disease. In this study we first compare the properties of Type I (GSK3357679A and MLi-2) and Type II (GZD-824, Rebastinib and Ponatinib) kinase inhibitors that bind to the closed or open conformations of the LRRK2 kinase domain, respectively. We show that Type I and Type II inhibitors suppress phosphorylation of Rab10 and Rab12, key physiological substrates of LRRK2 and also promote mitophagy, a process suppressed by LRRK2. Read More

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September 2021

An Overview of the Nrf2/ARE Pathway and Its Role in Neurodegenerative Diseases.

Int J Mol Sci 2021 Sep 4;22(17). Epub 2021 Sep 4.

Department of Cell-to-Cell Communication, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland.

Nrf2 is a basic region leucine-zipper transcription factor that plays a pivotal role in the coordinated gene expression of antioxidant and detoxifying enzymes, promoting cell survival in adverse environmental or defective metabolic conditions. After synthesis, Nrf2 is arrested in the cytoplasm by the Kelch-like ECH-associated protein 1 suppressor (Keap1) leading Nrf2 to ubiquitin-dependent degradation. One Nrf2 activation mechanism relies on disconnection from the Keap1 homodimer through the oxidation of cysteine at specific sites of Keap1. Read More

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September 2021

Expression Analysis of MaTGA8 Transcription Factor in Banana and Its Defence Functional Analysis by Overexpression in Arabidopsis.

Int J Mol Sci 2021 Aug 28;22(17). Epub 2021 Aug 28.

Institute of Horticulture Science and Engineering, Huaqiao University, Xiamen 361021, China.

TGA transcription factor is a member of the D subfamily of the basic region-leucine zippers (bZIP) family. It is a type of transcription factor that was first identified in plants and is the main regulator in plant development and physiological processes, including morphogenesis and seed formation in response to abiotic and biotic stress and maintaining plant growth. The present study examined the sequence of the transcription factor, the sequence of which belonged to subfamily D of the bZIP and had multiple cis-acting elements such as the G-box, TCA-element, TGACG-element, and P-box. Read More

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HLA-DRB1 and HLA-DQB1 genetic diversity modulates response to lithium in bipolar affective disorders.

Sci Rep 2021 Sep 8;11(1):17823. Epub 2021 Sep 8.

Department of Psychiatry & Center of Sleep Disorders, National Taiwan University Hospital, Taipei, Taiwan.

Bipolar affective disorder (BD) is a severe psychiatric illness, for which lithium (Li) is the gold standard for acute and maintenance therapies. The therapeutic response to Li in BD is heterogeneous and reliable biomarkers allowing patients stratification are still needed. A GWAS performed by the International Consortium on Lithium Genetics (ConLiGen) has recently identified genetic markers associated with treatment responses to Li in the human leukocyte antigens (HLA) region. Read More

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September 2021

Microbial biomass and activity dynamics in restored lands in a metal contaminated region.

Ecotoxicology 2021 Sep 8. Epub 2021 Sep 8.

Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada.

Soil microbial communities are important for biogeochemical processes, along with the cycling of nutrients in an ecosystem. Their enzymatic activities are key indicators of their responses to stress. The objective of this research was to assess the effect of land reclamation on microbial biomass and activities in soils impacted by metal contamination. Read More

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September 2021

[Responses of soil extracellular enzyme activities to the anthropogenic transition from desert grassland to shrubland in eastern Ningxia, China].

Ying Yong Sheng Tai Xue Bao 2020 Aug;31(8):2541-2548

College of Agriculture, Ningxia University, Yinchuan 750021, China.

To understand the responses of soil extracellular enzyme activities to the desert grassland-shrubland anthropogenic transition, we examined soil properties and six extracellular enzyme activities, in soil under vegetation patches and bare interspaces in desert grassland, grassland edge, shrubland edge, shrubland in a typical anthropogenic desert grassland-shrubland mosaic in desert steppe of eastern Ningxia, China. The six measured enzymes included cellobiohydrolase, β-1,4-xylosidase, β-1,4-glucosidase, β-1,4-N-acetylglucosaminidase, leucine aminopeptidase and alkaline phosphatase. We found that soil moisture, soil organic carbon, total nitrogen, total phosphorus, microbial biomass carbon and microbial biomass nitrogen decreased significantly by 26. Read More

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[Kinetic parameters and temperature sensitivity of soil nitrogen and phosphorus transforming enzymes in plantations under different vegetation zones on the Loess Plateau, China].

Ying Yong Sheng Tai Xue Bao 2020 Aug;31(8):2515-2522

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, Shaanxi, China.

Soil enzymes are catalysts for organic matter decomposition, the kinetic characteristics of which are important indicators of the catalytic performance of enzymes, with important role in evalua-ting soil health quality. We examined the responses of soil enzyme kinetic parameters to temperature change and the variation characteristics of their temperature sensitivity () in plantation soil under three different vegetation zones on the Loess Plateau. The results showed that the potential maximum reaction rate () and the half-saturation constant () of alanine transaminase (ALT), leucine aminopeptidase (LAP) and alkaline phosphatase (ALP) all increased linearly with the increasing incubation temperature. Read More

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[Stoichiometry of soil extracellular enzymes and its seasonal variation in natural forests with different altitudes in northern Greater Khingan Mountains, China].

Ying Yong Sheng Tai Xue Bao 2020 Aug;31(8):2491-2499

Ministry of Education Key Laboratory of Sustainable Forest Ecosystem Management, School of Forestry, Northeast Forestry University, Harbin 150040, China.

In this study, we examined the characteristics and influence mechanism of soil extracellular enzyme activity (EEA) and enzymatic stoichiometry in different soils in forests at different altitudes (750-1420 m) in Aokelidui Mountains in the north of the Greater Khingan Mountains. The results showed that altitude, season and their interactions significantly affected the activities of β-glucosidase (BG), β-1,4-N-acetylglucosaminidase (NAG), L-leucine aminopeptidase (LAP), and acid phosphatase (AP). In May, BG and NAG activities gradually increased with increasing altitude, while AP activities increased first and then decreased with increasing altitude. Read More

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TaFDL2-1A interacts with TabZIP8-7A protein to cope with drought stress via the abscisic acid signaling pathway.

Plant Sci 2021 Oct 14;311:111022. Epub 2021 Aug 14.

State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

Drought has negative effects on cereal production. Studies have shown that many basic leucine zipper transcription factors (bZIP TFs) help to cope with drought stress. In this study, bZIP TF wheat (Triticum aestivum L. Read More

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October 2021

Identification of anthracnose race 1 resistance loci in lentil by integrating linkage mapping and genome-wide association study.

Plant Genome 2021 Sep 5:e20131. Epub 2021 Sep 5.

Crop Development Centre, Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada.

Anthracnose, caused byColletotrichum lentis, is a devastating disease of lentil (Lens culinaris Medik.) in western Canada. Growing resistant lentil cultivars is the most cost-effective and environmentally friendly approach to prevent seed yield losses that can exceed 70%. Read More

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September 2021

Regulatory mechanisms of heme regulatory protein BACH1: a potential therapeutic target for cancer.

Med Oncol 2021 Sep 4;38(10):122. Epub 2021 Sep 4.

Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India.

A limited number of overexpressed transcription factors are associated with cancer progression in many types of cancer. BTB and CNC homology 1 (BACH1) is the first mammalian heme-binding transcription factor that belongs to the basic region leucine zipper (bZIP) family and a member of CNC (cap 'n' collar). It forms heterodimers with the small musculoaponeurotic fibrosarcoma (MAF) proteins and stimulates or suppresses the expression of target genes under a very low intracellular heme concentration. Read More

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September 2021

Comparative analysis of the MyTH4-FERM myosins reveals insights into the determinants of actin track selection in polarized epithelia.

Mol Biol Cell 2021 Sep 2:mbcE20070494. Epub 2021 Sep 2.

Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606.

MyTH4-FERM (MF) myosins evolved to play a role in the creation and function of a variety of actin-based membrane protrusions that extend from cells. Here, we performed an analysis of the MF myosins, Myo7A, Myo7B, and Myo10, to gain insight into how they select for their preferred actin networks. Using enterocytes that create spatially separated actin tracks in the form of apical microvilli and basal filopodia, we show that actin track selection is principally guided by the mode of oligomerization of the myosin along with the identity of the motor domain, with little influence from the specific composition of the lever arm. Read More

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September 2021

[Structure prediction and biological activity analysis of dybowskin-1ST antimicrobial peptide in Rana dybowskii].

Sheng Wu Gong Cheng Xue Bao 2021 Aug;37(8):2890-2902

School of Medicine, Beihua University, Jilin 132013, Jilin, China.

The evolution, structure and antigenic epitopes prediction of Rana dybowskii antimicrobial peptide dybowskin-1ST were carried out using bioinformatics software available online. Its antibacterial mechanism and structural properties were analyzed, and its activity was verified by applying wound healing assay in mice and bacteriostatic assay in vitro. This provides the theoretical basis for the improvement of parental peptide and the development of novel derivative peptides. Read More

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Phospholipase D2 is a positive regulator of sirtuin 1 and modulates p53-mediated apoptosis via sirtuin 1.

Exp Mol Med 2021 Sep 1. Epub 2021 Sep 1.

College of Pharmacy, Yonsei University, Incheon, 21983, South Korea.

Sirtuin 1 (SIRT1) is a nicotinamide adenine dinucleotide-dependent histone deacetylase that plays diverse physiological roles. However, little is known about the regulation of SIRT1 activity. Here, we show that phospholipase D2 (PLD2), but not PLD1, selectively interacts with SIRT1 and increases the deacetylase activity of SIRT1. Read More

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September 2021

Late adolescence mortality in mice with brain-specific deletion of the volume-regulated anion channel subunit LRRC8A.

FASEB J 2021 Oct;35(10):e21869

Department of Neuroscience and Experimental Therapeutics, Albany Medical College, Albany, New York, USA.

The leucine-rich repeat-containing family 8 member A (LRRC8A) is an essential subunit of the volume-regulated anion channel (VRAC). VRAC is critical for cell volume control, but its broader physiological functions remain under investigation. Recent studies in the field indicate that Lrrc8a disruption in the brain astrocytes reduces neuronal excitability, impairs synaptic plasticity and memory, and protects against cerebral ischemia. Read More

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October 2021

Genetics of Parkinson's disease in Brazil: a systematic review of monogenic forms.

Arq Neuropsiquiatr 2021 07;79(7):612-623

Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Neurociências e Ciências do Comportamento, Ribeirão Preto SP, Brazil.

Background: Increasing numbers of mutations causing monogenic forms of Parkinson's disease (PD) have been described, mostly among patients in Europe and North America. Since genetic architecture varies between different populations, studying the specific genetic profile of Brazilian patients is essential for improving genetic counseling and for selecting patients for clinical trials.

Objective: We conducted a systematic review to identify genetic studies on Brazilian patients and to set a background for future studies on monogenic forms of PD in Brazil. Read More

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Effects of iron, Vitamin A and the interaction between the two nutrients on intestinal development and cell differentiation in piglets.

J Anim Sci 2021 Sep 1. Epub 2021 Sep 1.

Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.

This study aimed to investigate the effects of iron, vitamin A (VA) and their interaction on intestinal development and differentiation of cells in suckling piglets. Therefore, 32 Duroc × Landrace × Yorkshire 0-day-old newborn boars with similar body weights were randomly divided into 4 groups, with 8 replicates in each group and 1 pig in each replicate. All the piglets were breastfed. Read More

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September 2021

Regulation and function of AP-1 in insulinoma cells and pancreatic β-cells.

Biochem Pharmacol 2021 Aug 27;193:114748. Epub 2021 Aug 27.

Saarland University Medical Faculty, Department of Medical Biochemistry and Molecular Biology, D-66421 Homburg, Germany. Electronic address:

Ca1.2 L-type voltage-gated Ca channels play a central role in pancreatic β-cells by integrating extracellular signals with intracellular signaling events leading to insulin secretion and altered gene transcription. Here, we investigated the intracellular signaling pathway following stimulation of Ca1. Read More

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Nuclear Magnetic Resonance-Based Metabolomic Analysis Reveals Physiological Stage, Breed, and Diet Effects on the Intramuscular Metabolism of Amino Acids and Related Nutrients in Pigs.

Front Vet Sci 2021 10;8:681192. Epub 2021 Aug 10.

Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.

Skeletal muscle is a complex tissue that exhibits considerable plasticity in response to nutrients, animal, or its growth stage, but the underlying mechanisms are largely unknown. This study was conducted to evaluate the effects of physiological stage, breed, and diet on the metabolome of the skeletal muscle of pigs. Ninety-six barrows, including 48 purebred Bama mini-pigs, representing the fat type, and 48 Landrace pigs, representing the lean type, were randomly assigned to either a low- or adequate-protein diet ( = 24 per group). Read More

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Nucleobindin-2 consists of two structural components: The Zn-sensitive N-terminal half, consisting of nesfatin-1 and -2, and the Ca-sensitive C-terminal half, consisting of nesfatin-3.

Comput Struct Biotechnol J 2021 30;19:4300-4318. Epub 2021 Jul 30.

Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.

Nucleobindin-2 (Nucb2) is a protein that has been suggested to play roles in a variety of biological processes. Nucb2 contains two Ca/Mg-binding EF-hand domains separated by an acidic amino acid residue-rich region and a leucine zipper. All of these domains are located within the C-terminal half of the protein. Read More

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Black tea is more effective than green tea in prevention of radiation-induced oxidative stress in the aorta of rats.

Biomed Pharmacother 2021 Oct 20;142:112064. Epub 2021 Aug 20.

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Institutskaya 3, 142290, Russia. Electronic address:

In the work, the effect of black tea on oxidative stress induced in the aorta by irradiation was studied. The efficiency of black and green tea types was compared, and the effect of the main green tea components (-)-epigallocatechin galate (EGCG) and (-)-epigallocatechin (EGC) on the aorta was studied. The activity of ACE in rat aorta segments was determined by measuring the hydrolysis of hippuryl-L-histidyl-L-leucine, and the production of ROS was estimated from the oxidation of dichlorodihydrofluorescein. Read More

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October 2021

Genome-wide association study reveals a quantitative trait locus and two candidate genes on Sus scrofa chromosome 5 affecting intramuscular fat content in Suhuai pigs.

Animal 2021 Sep 21;15(9):100341. Epub 2021 Aug 21.

Institute of Swine Science, Nanjing Agricultural University, Nanjing 210095, China; Huaian Academy, Nanjing Agricultural University, Huaian 223005, China. Electronic address:

Intramuscular fat content (IFC) is an essential quantitative trait of meat, affecting multiple meat quality indicators. A certain amount of IFC could not only improve the sensory score of pork but also increase the flavour, tenderness, juiciness and shelf-life. To dissect the genetic determinants of IFC, two methods, including genome-wide efficient mixed-model analysis (GEMMA) and linkage disequilibrium adjusted kinships (LDAKs), were used to carry out genome-wide association studies for IFC in Suhuai pig population. Read More

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September 2021

Age-related LRRK2 G2019S Mutation Impacts Microglial Dopaminergic Fiber Refinement and Synaptic Pruning Involved in Abnormal Behaviors.

J Mol Neurosci 2021 Aug 19. Epub 2021 Aug 19.

Department of Neurology, Fujian Medical University Union Hospital, 29 Xinquan Road, Fuzhou, 350001, China.

Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are the most frequent cause of autosomal dominant Parkinson's disease (PD), producing psychiatric and motor symptoms. We conducted this study to explore whether microglial dopaminergic (DAergic) fiber refinement and synaptic pruning are involved in the abnormal behavioral phenotypes of carriers of the LRRK2 G2019S mutation, by employing young and middle-aged PD model mice. The results revealed a characteristic late-onset hyperactivity and a progressive decline in the motor coordination of the LRRK2 G2019S mutation mice. Read More

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Conserved L464 in p97 D1-D2 linker is critical for p97 cofactor regulated ATPase activity.

Biochem J 2021 Sep;478(17):3185-3204

Division of Medical Genetics, Department of Pediatrics, Harbor-UCLA Medical Center and The Lundquist Institute, Torrance, California 90502, U.S.A.

p97 protein is a highly conserved, abundant, functionally diverse, structurally dynamic homohexameric AAA enzyme-containing N, D1, and D2 domains. A truncated p97 protein containing the N and D1 domains and the D1-D2 linker (ND1L) exhibits 79% of wild-type (WT) ATPase activity whereas the ND1 domain alone without the linker only has 2% of WT activity. To investigate the relationship between the D1-D2 linker and the D1 domain, we produced p97 ND1L mutants and demonstrated that this 22-residue linker region is essential for D1 ATPase activity. Read More

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September 2021

LncRNA FBXL19-AS1 promotes proliferation and metastasis of cervical cancer through upregulating COL1A1 as a sponge of miR-193a-5p.

J Biol Res (Thessalon) 2021 Aug 16;28(1):20. Epub 2021 Aug 16.

Department of Laboratory, Xinjiang Uygur Autonomous Region Maternal and Child Health Hospital, No. 1 Renmin Road, Ürümqi, 830000, Xinjiang, People's Republic of China.

Background: Cervical cancer (CC) is one of the most common and malignant tumors in women. In this study, we aim to explore the role and mechanism of F-box and leucine rich repeat protein 19 antisense RNA 1 (FBXL19-AS1), a novel long-chain non coding RNA (lncRNA) with marked roles in a variety of tumors, in regulating the proliferation and metastasis of CC.

Methods: The expression of FBXL19-AS1, miR-193a-5p and COL1A1 were detected by RT-PCR and western blot. Read More

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The intragenic suppressor mutation Leu59Phe compensates for the effect of detrimental mutations in the jasmonate receptor COI1.

Plant J 2021 Aug 15. Epub 2021 Aug 15.

Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

The phytohormones jasmonates (JAs) control plant development, growth, and defense against insects and pathogens. The Arabidopsis JA receptor Coronatine Insensitive 1 (COI1) interacts with ARABIDOPSIS SKP-LIKE1 (ASK1)/ASK2 to form the SCF E3 ligase and mediate JA responses. Here, we performed a genetic suppressor screen using the leaky coi1-2 (COI1 ) mutant for restored sensitivity to JA, and identified the intragenic suppressor mutation Leu59Phe, which was in the region connecting the F-box and leucine-rich repeats domains of COI1. Read More

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Virtual Screening and Biological Activity Evaluation of New Potent Inhibitors Targeting LRRK2 Kinase Domain.

ACS Chem Neurosci 2021 09 13;12(17):3214-3224. Epub 2021 Aug 13.

College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

Leucine rich repeat kinase 2 (LRRK2) has been reported in the pathogenesis of Parkinson's disease (PD). Inhibition of LRRK2 kinase activity is a therapeutic approach that may provide new treatments for PD. In this study, novel LRRK2 inhibitors were identified by performing a docking-based virtual screening (VS). Read More

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September 2021