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Development of highly sensitive one step-PCR tests for improved detection of B. bigemina and B. bovis.

Vet Parasitol 2021 Jun 8;296:109493. Epub 2021 Jun 8.

Instituto de Agrobiotecnología y Biología Molecular (IABIMO) INTA - CONICET, de Los Reseros y Dr. Nicolás Repetto s/n, P.O. Box 25 (B1712WAA), Castelar, Buenos Aires, Argentina.

Bovine babesiosis caused by Babesia bigemina and B. bovis is an economically relevant tick-borne disease distributed over tropical and subtropical world regions. Animals that recover from the clinical disease can remain persistently infected, and those carriers are epidemiologically relevant since they can act as a source of infection to other animals through the tick bite. Read More

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Phylogenetics and comparative plastome genomics of two of the largest genera of angiosperms, Piper and Peperomia (Piperaceae).

Mol Phylogenet Evol 2021 Jun 13;163:107229. Epub 2021 Jun 13.

Department of Biological Sciences, Boise State University, 1910 University Drive, Boise, ID 83725-1515, USA. Electronic address:

Biological radiations provide unique opportunities to understand the evolution of biodiversity. One such radiation is the pepper plant family Piperaceae, an early-diverging and mega-diverse lineage that could serve as a model to study the diversification of angiosperms. However, traditional genetic markers lack sufficient variation for such studies, and testing hypotheses on poorly resolved phylogenetic frameworks becomes challenging. Read More

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In silico screening and exploration into phenotypic alterations of deleterious oncogenic single nucleotide polymorphisms in HSPB1 gene.

Genomics 2021 Jun 12. Epub 2021 Jun 12.

Amity Institute of Biotechnolgy, Amity University Kolkata, India. Electronic address:

A small heat shock protein, HSP27, encoded by HSPB1 gene strongly favors survival, proliferation and metastasis of cancer cells and its expression is dependent on post-translational modifications like phosphorylation. This study performed an extensive in silico screening of 20 deleterious non-synonymous SNPs in the coding region of HSPB1 gene, among which four were identified to be cancer associated. The SNP variant I181S introduced a new phosphorylation site in position 181, which might elevate the protein's activation potential. Read More

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A multicenter study to develop a non-invasive radiomic model to identify urinary infection stone in vivo using machine-learning.

Kidney Int 2021 Jun 12. Epub 2021 Jun 12.

Department of Pharmacy, the First People's Hospital of Kashi Prefecture; Affiliated Kashi Hospital of Sun Yat-Sen University, Kashi, People's Republic of China. Electronic address:

Urolithiasis is a common urological disease, and treatment strategy options vary between different stone types. However, accurate detection of stone composition can only be performed in vitro. The management of infection stones is particularly challenging with yet no effective approach to pre-operatively identify infection stones from non-infection stones. Read More

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Kaempferol inhibits benign prostatic hyperplasia by resisting the action of androgen.

Eur J Pharmacol 2021 Jun 12:174251. Epub 2021 Jun 12.

Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Poyang Lake Road 10, Tianjin 301617, China; Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Poyang Lake Road 10, Tianjin 301617, China; Laboratory of Pharmacology of TCM Formulae Co-Constructed By the Province-Ministry, Tianjin University of TCM, Poyang Lake Road 10, Tianjin 301617, China. Electronic address:

Kaempferol is a natural compound that inhibits tumor development in androgenic related prostate cancer. However, it is still not clear about its phyto-androgenic activity and whether it suppresses testosterone-induced benign prostatic hyperplasia (BPH) development. In this study, molecular docking, cellular immunofluorescence staining, chromatin immunoprecipitation and dual luciferase reporter assay were performed to investigate the androgenic activity of kaempferol. Read More

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Intraspecific variations of the cytoplasmic male sterility genes and in Brassica maurorum and Moricandia arvensis, and the specificity of the mRNA processing.

Genome 2021 Jun 15. Epub 2021 Jun 15.

Dept. Biotech., Fac. Eng., Kyoto Sangyo Univ., Motoyama, Kamigamo, Kyoto, Kyoto, Japan, 603-8555;

The mitochondrial gene co-transcribed with and causes cytoplasmic male sterility in crops, is widely distributed across wild species and genera of . However, intraspecific variations in the presence of have not yet been studied, and the mechanisms for the wide distribution of the gene remain unclear. We analyzed the presence and sequence variations of in two wild species, and . Read More

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Spatial-temporal dynamics and influencing factors of archaeal communities in the sediments of Lancang River cascade reservoirs (LRCR), China.

PLoS One 2021 15;16(6):e0253233. Epub 2021 Jun 15.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, China.

The spatial and temporal distribution of the archaeal community and its driving factors in the sediments of large-scale regulated rivers, especially in rivers with cascade hydropower development rivers, remain poorly understood. Quantitative PCR (qPCR) and Illumina MiSeq sequencing of the 16S rRNA archaeal gene were used to comprehensively investigate the spatiotemporal diversity and structure of archaeal community in the sediments of the Lancang River cascade reservoirs (LRCR). The archaeal abundance ranged from 5. Read More

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Physiological Changes and Differential Gene Expression of Tea Plants ( (L.) Kuntze var. Q.H. Chen) Under Cold Stress.

DNA Cell Biol 2021 Jun 15. Epub 2021 Jun 15.

The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, China.

Low temperature is an important factor that affects the growth and reproduction of tea plants [ (L.) Kuntze]. In this study, Yunwu Tribute Tea cutting seedlings [ (L. Read More

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Genetic Analysis Reveals Relationships Among Populations of Puccinia striiformis f. sp. tritici from the Longnan, Longdong and Central Shaanxi Regions of China.

Phytopathology 2021 Jun 15. Epub 2021 Jun 15.

Northwest A&F University, State Key Laboratory of Crop Stress Biology for Arid Areas, Xinong Road #22, Yangling, Shaanxi, China, 712100.

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most important diseases of wheat worldwide. Read More

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First Report of Brown Spot Needle Blight on Pinus thunbergii Parl. Caused by Aureobasidium pullulans in China.

Plant Dis 2021 Jun 15. Epub 2021 Jun 15.

Nanjing Forestry University, 74584, College of Forestry, 159 Longpan Road, Nanjing, China, 210037;

Pinus thunbergii Parl., known as black pine, is widely distributed all over China. This pine variety can prevent soil desertification and promote soil conservation and is excellent for constructing fast-growing forests and shelter belts. Read More

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Mitochondrial genome variations are associated with amyotrophic lateral sclerosis in patients from mainland China.

J Neurol 2021 Jun 15. Epub 2021 Jun 15.

Department of Neurology, Xiangya Hospital, Central South University, 87 Xiangya Rd, Changsha, Hunan, People's Republic of China.

Background: Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder. Mitochondrial dysfunction is involved in the complex pathophysiology of ALS; however, the role of mitochondrial DNA (mtDNA) variants in ALS is poorly understood. We aimed to elucidate the role of mtDNA variants in the pathogenesis of ALS. Read More

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Inferring Population Histories for Ancient Genomes Using Genome-Wide Genealogies.

Mol Biol Evol 2021 Jun 15. Epub 2021 Jun 15.

Department of Statistics, University of Oxford, Oxford, UK.

Ancient genomes anchor genealogies in directly observed historical genetic variation and contextualise ancestral lineages with archaeological insights into their geography and cultural associations. However, the majority of ancient genomes are of lower coverage and cannot be directly built into genealogies. Here, we present a fast and scalable method, Colate, the first approach for inferring ancestral relationships through time between low-coverage genomes without requiring phasing or imputation. Read More

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Phenotypic spectrum and mechanism analysis of Schaff Yang syndrome: A case report on new mutation of MAGEL2 gene.

Medicine (Baltimore) 2021 Jun;100(24):e26309

Department of Endocrinology, Shandong Provincial Hospital affiliated to Shandong First Medical University.

Rationale: The Schaaf-Yang syndrome (SYS) is an autosomal dominant multi-system genetic disease caused by melanoma antigen L2 (MAGEL2) gene mutations imprinted by mothers and expressed by fathers on the 15q11-15q13 chromosomes in the critical region of Prader-Willi. MAGEL2 is a single exon gene and one of the protein-coding genes of the Prader-Willi domain. MAGEL2 is a matrilineal imprinted gene (i. Read More

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Long noncoding RNA taurine-up regulated gene 1 for the prognosis of osteosarcoma: A protocol for meta-analysis and bioinformatics analysis.

Medicine (Baltimore) 2021 Jun;100(24):e26182

Department of Orthopedics and Soft Surgery, Gansu Cancer Hospital, Lanzhou, Gansu Province, China.

Background: In recent years, a variety of long noncoding RNA (lncRNA) has been confirmed to be involved in the initiation and progression of osteosarcoma. Taurine-up regulated gene 1 (TUG1) plays an important role in the formation, invasion, and metastasis of osteosarcoma. Therefore, perhaps TUG1 is a potential biomarker for the prognosis of patients suffering from osteosarcoma. Read More

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Correlation between single nucleotide polymorphisms in the 3 primer untranslated region of PTX3 and the risk of essential hypertension: A case-control study.

Medicine (Baltimore) 2021 Jun;100(24):e25937

Department of Clinical Laboratory, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.

Abstract: The aim of this study was to investigate the correlation between single-nucleotide polymorphisms (SNPs) in the 3 primer of untranslated region (3'UTR) of the Pentraxin 3 (PTX3) gene and the risk of essential hypertension (EHT).PTX3 genotypes, rs2614, rs111451363, and rs73158510 locus, were found in 260 patients with EHT and 260 healthy controls. Quantitative real-time polymerase chain reaction was used to detect plasma hsa-miR-4766-5p levels. Read More

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Insights into evolution and coexistence of the colibactin- and yersiniabactin secondary metabolite determinants in enterobacterial populations.

Microb Genom 2021 Jun;7(6)

Institute of Hygiene, University of Münster, Münster, Germany.

The bacterial genotoxin colibactin interferes with the eukaryotic cell cycle by causing dsDNA breaks. It has been linked to bacterially induced colorectal cancer in humans. Colibactin is encoded by a 54 kb genomic region in . Read More

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Isolation and genetic characterization of canine adenovirus type 2 from a domestic dog showing neurological symptoms.

Braz J Microbiol 2021 Jun 14. Epub 2021 Jun 14.

ICAR-IVRI, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.

Canine adenoviruses (CAVs) are of two types: canine adenovirus type 1 (CAV-1), which causes infectious canine hepatitis, and canine adenovirus type 2 (CAV-2), which is mainly associated with the respiratory type of disease in dogs. Due to the widespread use of modified live vaccines to control canine adenoviral infections and subsequently reduced disease incidence, CAVs are often neglected by clinicians. Although a number of studies are available about CAV-1 prevalence in India, only meagre information is available about CAV-2. Read More

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Genome-Wide Identification of MATE Gene Family in Potato ( L.) and Expression Analysis in Heavy Metal Stress.

Front Genet 2021 28;12:650500. Epub 2021 May 28.

College of Agricultural, Guizhou University, Guiyang, China.

A genome-wide identification and expression analysis of multidrug and toxic compound extrusion (MATE) gene family in potato was carried out to explore the response of MATE proteins to heavy meta stress. In this study, we identified 64 MATE genes from potato genome, which are located on 12 chromosomes, and are divided into I-IV subfamilies based on phylogenetic analysis. According to their order of appearance on the chromosomes, they were named from . Read More

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Independent duplications of the Golgi phosphoprotein 3 oncogene in birds.

Sci Rep 2021 Jun 14;11(1):12483. Epub 2021 Jun 14.

Integrative Biology Group, Universidad Austral de Chile, Valdivia, Chile.

Golgi phosphoprotein 3 (GOLPH3) was the first reported oncoprotein of the Golgi apparatus. It was identified as an evolutionarily conserved protein upon its discovery about 20 years ago, but its function remains puzzling in normal and cancer cells. The GOLPH3 gene is part of a group of genes that also includes the GOLPH3L gene. Read More

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Oxysterols Modify NLRP2 in Epithelial Cells, Identifying a Mediator of Ozone-induced Inflammation.

Am J Respir Cell Mol Biol 2021 Jun 14. Epub 2021 Jun 14.

University of North Carolina, Pediatrics, Chapel Hill, North Carolina, United States;

Ozone (O) is a prevalent air pollutant causing lung inflammation. Previous studies demonstrate that O oxidizes lipids, such as cholesterol, in the airway to produce oxysterols, such as secosterol-A (SecoA), which are electrophiles capable of forming covalent linkages preferentially with lysine residues and consequently modify protein function. The breadth of proteins modified by this oxysterol as well as the biological consequences in the lung are unknown. Read More

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Sequence subtyping of from Bonelli's eagle () during four years (2014-2017) reveals that MLS type is associated with lesions.

Avian Pathol 2021 Jun 15:1-58. Epub 2021 Jun 15.

Departament of Animal Health, Faculty of Veterinary Medicine, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain.

Avian trichomonosis is a parasitic disease that affects wild birds, The objective of this work was to determine the importance of avian trichomonosis in Bonelli's eagles to improve conservation measures in this population. One hundred and eighty-eight birds were studied: 181 chicks,, two juveniles, one subadult and four adults. The birds were externally examined and gross lesions at the oropharynx registered. Read More

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Loss of cGMP-dependent protein kinase II alters ultrasonic vocalizations in mice, a model for speech impairment in human microdeletion 4q21 syndrome.

Neurosci Lett 2021 Jun 12;759:136048. Epub 2021 Jun 12.

Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA; Molecular, Cellular and Integrative Neurosciences Program, Colorado State University, Fort Collins, CO 80523, USA. Electronic address:

Chromosome 4q21 microdeletion leads to a human syndrome that exhibits restricted growth, facial dysmorphisms, mental retardation, and absent or delayed speech. One of the key genes in the affected region of the chromosome is PRKG2, which encodes cGMP-dependent protein kinase II (cGKII). Mice lacking cGKII exhibit restricted growth and deficits in learning and memory, as seen in the human syndrome. Read More

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sRNA-mediated RNA processing regulates bacterial cell division.

Nucleic Acids Res 2021 Jun 14. Epub 2021 Jun 14.

Institute of Microbiology and Molecular Biology, University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.

Tight control of cell division is essential for survival of most organisms. For prokaryotes, the regulatory mechanisms involved in the control of cell division are mostly unknown. We show that the small non-coding sRNA StsR has an important role in controlling cell division and growth in the alpha-proteobacterium Rhodobacter sphaeroides. Read More

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In silico analysis of deleterious SNPs of human MTUS1 gene and their impacts on subsequent protein structure and function.

PLoS One 2021 14;16(6):e0252932. Epub 2021 Jun 14.

Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh.

The mitochondrial tumor suppressor 1 (MTUS1) gene acts as a crucial tumor suppressor by inhibiting growth and proliferation of eukaryotic cells including tumor cell lines. Down regulation of MTUS1 gene has been implicated in a wide range of cancers as well as various human diseases. Alteration through nsSNPs can potentially damage the structure and/or function of the protein. Read More

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Epidemic and PCR-based identification of vibrio cholera through OmpW gene from diarrhoeal patients admitted at different hospitals of Baluchistan.

J Pak Med Assoc 2021 Apr;71(4):1189-1192

Department of Biochemistry, Bolan University of Medical and Health Sciences, Quetta, Pakistan.

Objective: To study the different epidemiological and polymerase chain reaction-based identification of vibrio cholera.

Methods: The cross-sectional study was conducted at the Center for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, Pakistan, from January 5 to December 6, 2019, and comprised faecal / rectal swab samples from patients with a history of untreated severe diarrhoea of <12-hour duration. The samples were collected from suspected cholera patients at different hospitals of the province. Read More

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Genomic evolution and virulence association of sequence type 37 (ribotype 017) in China.

Emerg Microbes Infect 2021 Jun 14:1-0. Epub 2021 Jun 14.

School of Laboratory Medicine, Hangzhou Medical College, Hangzhou, Zhejiang, 310053, China.

sequence type (ST) 37 (ribotype 017) is one of the most prevalent genotypes circulating in China. However, its genomic evolution and virulence determinants were rarely explored. Whole-genome sequencing, phylogeographic and phylogenetic analyses were conducted for ST37 isolates. Read More

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GmhB enzyme involved in ADP-heptose biosynthesis pathway is essential for lipopolysaccharide biosynthesis and bacterial virulence.

Virulence 2021 Dec;12(1):1610-1628

Institute of Molecular Medicine, College of Life Science, National Tsing Hua University, Hsinchu, Taiwan.

infection is linked to serious gastric-related diseases including gastric cancer. However, current therapies for treating infection are challenged by the increased antibiotic resistance of . Therefore, it is in an urgent need to identify novel targets for drug development against infection. Read More

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December 2021

The miR-302c/transforming growth factor-β receptor type-2 axis modulates interleukin-1β-induced degenerative changes in osteoarthritic chondrocytes.

J Cell Commun Signal 2021 Jun 14. Epub 2021 Jun 14.

Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, People's Republic of China.

Chondrocyte production of catabolic and inflammatory mediators participating in extracellular matrix degradation has been regarded as a central event in osteoarthritis (OA) development. During OA pathogenesis, interleukin-1β (IL-1β) decreases the mRNA expression and protein levels of transforming growth factor-β receptor type-2 (TGFBR2), thus disrupting transforming growth factor-β signaling and promoting OA development. In the present study, we attempted to identify the differentially expressed genes in OA chondrocytes upon IL-1β treatment, investigate their specific roles in OA development, and reveal the underlying mechanism. Read More

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A novel mutant allele at the Cleistogamy 1 locus in barley.

Theor Appl Genet 2021 Jun 14. Epub 2021 Jun 14.

Institute of Crop Science, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.

Key Message: A chasmogamous mutant was induced by exposing a cleistogamous cultivar to sodium azide. The altered cly1 sequence in the mutant was not in the miR172 binding site, as is the case in other known cleistogamous alleles, but rather in a region encoding one of the gene product's two AP2 domains. The genetic basis of cleistogamy (in which pollination occurs before the flower opens) in barley is centered on the Cleistogamy 1 locus (cly1). Read More

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Endocrine Dysfunction in Patients with Myotonic Dystrophy.

J Clin Endocrinol Metab 2021 Jun 14. Epub 2021 Jun 14.

Division of Endocrinology, Metabolism and Diabetes, University of Louisville, Louisville, KY.

Myotonic dystrophy is a dominantly inherited multisystem disorder that results from increased CTG repeats in the 3' region of the myotonic dystrophy protein kinase gene (DMPK). The mutant DMPK mRNA remains nuclear, and sequesters RNA-binding proteins including regulators of mRNA splicing. Myotonic dystrophy is characterized by a highly variable phenotype that includes muscle weakness and myotonia, and may affect the function of many Endocrine glands. Read More

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