47,819 results match your criteria reference sera


Percentile values for serum levels of vitamins A and D in Iranian children and adolescents: The CASPIAN-V study.

Nutrition 2021 Apr 30;90:111307. Epub 2021 Apr 30.

Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.

Objectives: The reference values to determine vitamin A and D deficiency may vary based on ethnicity, age, and region of residence. The aim of this study was to determine the reference interval of circulating vitamin A and D levels among Iranian children and adolescents based on sex, age, and region of residence.

Methods: This nationwide cross-sectional study was performed with 2596 students 7 to 18 y of age from the CASPIAN-V (Childhood and Adolescence Surveillance and Prevention of Adult Non- communicable disease) study. Read More

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Altered neuroaxonal integrity in schizophrenia and major depressive disorder assessed with neurofilament light chain in serum.

J Psychiatr Res 2021 Jun 2;140:141-148. Epub 2021 Jun 2.

Department of Psychiatry, Psychotherapy and Psychosomatics, Psychiatric University Hospital Zürich, University of Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and Swiss Federal Institute of Technology Zurich, Zurich, Switzerland.

Background: Schizophrenia (SZ) and major depressive disorders (MDD) have been frequently linked to anatomical brain alterations. However, the relationship between brain pathology, inflammation and clinical symptoms in these disorders is still unclear. Thus, by applying novel blood markers of neuroaxonal integrity such as neurofilament light chain (NfL), we can now address main issues in psychiatric research and potentially offer innovative diagnostic tools toward better clinical characterizations and monitoring in both SZ and MDD. Read More

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Hydrolyzed seawater pearl tablet modulates the immunity via attenuating Th1/Th2 imbalance in an immunosuppressed mouse model.

J Tradit Chin Med 2021 Jun;41(3):397-405

School of Basic Medical Science, Guangxi University of Chinese Medicine, Nanning 530200, China.

Objective: To investigate whether Hydrolyzed Seawater Pearl tablet (HSPT) could modulate the Th1/Th2 imbalance in an immunosuppressed mouse model with Th1 to Th2 shift induced by Cyclosporine A (CsA) which can be used in the clinical treatment of Th2 to Th1 shift diseases, and explore the possible mechanism for the adjuvant therapeutic efficacy of HSPT on recurrent respiratory infections (RRI) and acquired immune deficiency syndrome (AIDS).

Methods: The mice were randomly divided into six groups of five animals each, namely normal group, model group, lentinan polysaccharide tablet (LPT) group and three HPST treated groups. HPST treated groups were administered with HPST (0. Read More

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Correlation of CD38 expression with the progression of hemorrhagic fever with renal syndrome.

Arch Virol 2021 Jun 10. Epub 2021 Jun 10.

Department of Clinical Laboratory, Xi'an Forth Hospital, 21 Jiefang Road, Xi'an, 710004, China.

To assess the relationship between the expression of CD38 and the progression of hemorrhagic fever with renal syndrome (HFRS), we determined the levels of CD38 during different phases of HFRS and evaluated the relationship between changes in CD38 expression and the progression of HFRS. The expression of CD38 in 68 patients with HFRS was analyzed by flow cytometry, and this method was also used to determine the levels of CD4T, CD8T, and B lymphocytes and NK cells. Furthermore, creatinine (Cr), uric acid (UA), and urea in serum at each stage of HFRS were measured using commercial kits. Read More

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Immunomics-Guided Antigen Discovery for Praziquantel-Induced Vaccination in Urogenital Human Schistosomiasis.

Front Immunol 2021 25;12:663041. Epub 2021 May 25.

Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.

Despite the enormous morbidity attributed to schistosomiasis, there is still no vaccine to combat the disease for the hundreds of millions of infected people. The anthelmintic drug, praziquantel, is the mainstay treatment option, although its molecular mechanism of action remains poorly defined. Praziquantel treatment damages the outermost surface of the parasite, the tegument, liberating surface antigens from dying worms that invoke a robust immune response which in some subjects results in immunologic resistance to reinfection. Read More

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Serum iPTH range in a reference population: from an integrated approach to vitamin D prevalence impact evaluation.

Clin Chim Acta 2021 Jun 7. Epub 2021 Jun 7.

Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy; Unit of Laboratory Medicine, Tor Vergata University Hospital, Rome, Italy.

Background: The iPTH upper reference limit (URL) reported by our laboratory provider (Abbott Laboratories) at Tor Vergata University Hospital was evaluated by internal verification procedures as not representative of our population and resulting as underestimated. In this study, a new reference interval has been investigated and established by comparing a direct and an indirect method based on a statistical reduction from results stored in the laboratory database.

Methods: For reference interval calculation from the healthy population, we analyzed a cohort of 100 blood donors (84% males and 16% females) screened with no bone-related and malabsorption diseases. Read More

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Evaluation of UMELISA CHAGAS™ with the incorporation of new monomeric and chimeric peptides representative of different regions of Trypanosoma cruzi

Biomedica 2021 05 31;41(Supl. 1):113-120. Epub 2021 May 31.

Centro de Inmunoensayo, La Habana, Cuba.

Introduction: Most people with Chagas disease develop specific antibodies against Trypanosoma cruzi. In early infection, IgM antibodies against T. cruzi are produced and later replaced for IgG antibodies during the course of the disease. Read More

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Development of Lentiviral Vectors Pseudotyped With Influenza B Hemagglutinins: Application in Vaccine Immunogenicity, mAb Potency, and Sero-Surveillance Studies.

Front Immunol 2021 24;12:661379. Epub 2021 May 24.

Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent & University of Greenwich, Chatham, United Kingdom.

Influenza B viruses (IBV) cause respiratory disease epidemics in humans and are therefore components of seasonal influenza vaccines. Serological methods are employed to evaluate vaccine immunogenicity prior to licensure. However, classical methods to assess influenza vaccine immunogenicity such as the hemagglutination inhibition assay (HI) and the serial radial hemolysis assay (SRH), have been proven to have many limitations. Read More

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Challenges in Measuring AMH in the Clinical Setting.

Front Endocrinol (Lausanne) 2021 24;12:691432. Epub 2021 May 24.

Discipline of Obstetrics & Gynaecology, School of Women's & Children's Health, University of New South Wales, Sydney, NSW, Australia.

Serum anti-Mullerian hormone (AMH) is a widely used marker of functional ovarian reserve in the assessment and treatment of infertility. It is used to determine dosing of gonadotropins used for superovulation prior to fertilization, as well as to determine the degree of damage to ovarian reserve by cytotoxic treatments such as chemotherapy. AMH is also now used to predict proximity to menopause and potentially provides a sensitive and specific test for polycystic ovarian syndrome. Read More

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Status of Four Trace Elements in Elderly Patients with Online Hemodiafiltration.

Clin Lab 2021 Jun;67(6)

Background: Essential trace elements play key roles in multiple biological systems, and hemodialysis patients are at risk for deficiency of essential trace elements. The aim of the study was to assess the essential element status in end stage renal disease patients undergoing online hemodiafiltration (online HDF) in outpatient dialysis clinic.

Methods: A total of 28 Korean patients with regular online HDF were included. Read More

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Serum TBA and GCA Levels Across Pregnancy and the Gestational Age-Specific Reference Intervals for Pregnant Chinese.

Clin Lab 2021 Jun;67(6)

Background: Reference intervals of clinical laboratory indexes are the basis of interpretation of test results. While intrahepatic cholestasis of pregnancy (ICP) could severely threaten maternal and fetal health, reference intervals of the biomarkers serum total bile acids (TBA) and glycocholic acid (GCA) are unsatisfactory. The purpose of this study was to derive gestational age-specific reference intervals for serum TBA and GCA in a Chinese population to promote early diagnosis of ICP. Read More

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Evidence-based Definition for Extensively Drug-resistant Tuberculosis.

Am J Respir Crit Care Med 2021 Jun 9. Epub 2021 Jun 9.

University of Milan, 9304, Centre for Multidisciplinary Research in Health Science, Milano, Italy.

Rationale: Until 2020, extensively drug-resistant tuberculosis (XDR-TB) was defined as resistance to rifampicin and isoniazid (multidrug-resistant tuberculosis, MDR-TB), any fluoroquinolone (FQ) and any second-line injectable drug (SLID). In 2019 the World Health Organization issued new recommendations for managing patients with drug-resistant tuberculosis, substantially limiting the role of SLID in MDR-TB treatment and thus putting that XDR-TB definition into question.

Objective: To propose an up-to-date definition for XDR-TB. Read More

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The Association between Elevated Carotid Intima-Media Thickness (CIMT) and Serum Uric Acid Levels (SUA) among Patients with Essential Hypertension in Primary Care Setting in Sungai Buloh, Malaysia.

Int J Clin Pract 2021 Jun 9:e14445. Epub 2021 Jun 9.

Sungai Buloh Health Clinic, Ministry of Health, Sungai Buloh, Selangor, Malaysia.

Aims: Our study aimed to investigate the association between elevated carotid-intima media thickness (CIMT) and serum uric acid (SUA) levels in hypertensive patients attending primary care clinics in Sungai Buloh, Malaysia.

Methods: We conducted a cross-sectional study on 140 hypertensive patients attending out-patient follow-up in two primary care clinics in Sungai Buloh, Malaysia, using a convenient sampling method. Serum uric acid levels were measured and divided into 4-quartile. Read More

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X-linked hypophosphatemia and burosumab: practical clinical points from the French experience.

Joint Bone Spine 2021 Jun 5:105208. Epub 2021 Jun 5.

APHP, Reference Center for Rare Diseases of Calcium and Phosphate Metabolism, and Filière OSCAR, Paris, France; APHP, Endocrinology and Diabetes for Children, Bicêtre Paris-Sud Hospital, Le Kremlin-Bicêtre, France. Electronic address:

Hereditary hypophosphatemia with increased FGF23 levels are rare inherited metabolic diseases characterized by low serum phosphate because of impaired renal tubular phosphate reabsorption. The most common form is X-linked hypophosphatemia (XLH), secondary to a mutation in the PHEX gene. In children, XLH is often manifested by rickets, delayed development of gait, lower limb deformities, growth retardation, craniosynostosis, and spontaneous dental abscesses. Read More

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A randomised, controlled trial to determine the effect of levothyroxine on Standardbred racehorses.

Equine Vet J 2021 Jun 8. Epub 2021 Jun 8.

Department of Veterinary Clinical Sciences, Purdue University College of Veterinary Medicine, West Lafayette, Indiana, 47907, USA.

Background: The use of thyroid supplement is pervasive in athletic horses although its effects on measures of performance are not known.

Objectives: One purpose of this study was to determine whether supra-physiologic doses of levothyroxine affect the velocities at which blood lactate was greater than 4 mmol/L (V ) and heart rate was over 150 (V ) and 200 (V ) beats per minute respectively. Additionally, a survey of post-race blood samples was also conducted to determine whether high thyroxine concentrations were common in racehorses. Read More

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Correlation analysis of low-level serum uric acid and cardiovascular events in patients on peritoneal dialysis.

Int Urol Nephrol 2021 Jun 8. Epub 2021 Jun 8.

Department of Nephrology, The First Affiliated Hospital of Nanchang University, 17# Yong wai street, Nanchang, 330006, China.

Background: The impact of serum uric acid (SUA) on development of cardiovascular disease (CVD) in patients undergoing peritoneal dialysis (PD) remains controversial, especially the impact of hypouricemia (HUA) on CVD. The aim of our study was to investigate the influence of low-level SUA on cardiovascular (CV) events in PD patients.

Methods: A retrospective cohort study was conducted. Read More

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Ultrafast LC-MS/MS analysis of 5-hydroxyindoleacetic acid (5-HIAA) in serum.

Scand J Clin Lab Invest 2021 Jun 8:1-5. Epub 2021 Jun 8.

Department of Laboratory Medicine, Örebro, Sweden.

A very quick and easy LC-MS/MS analysis method for 5-HIAA (5-hydoxyindoleacetic acid) has been developed. The method was fully validated and proved to work well in a clinical setting. Precision at the upper reference limit 123 nmol/L was 3,3% CV. Read More

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Plasma Mucin-1 (CA15-3) Levels in Autosomal Dominant Tubulointerstitial Kidney Disease due to MUC1 Mutations.

Am J Nephrol 2021 Jun 7:1-10. Epub 2021 Jun 7.

Department of Paediatrics and Inherited Metabolic Disorders, Research Unit of Rare Diseases, First Faculty of Medicine, Charles University, Prague, Czechia.

Introduction: Patients with ADTKD-MUC1 have one allele producing normal mucin-1 (MUC1) and one allele producing mutant MUC1, which remains intracellular. We hypothesized that ADTKD-MUC1 patients, who have only 1 secretory-competent wild-type MUC1 allele, should exhibit decreased plasma mucin-1 (MUC1) levels. To test this hypothesis, we repurposed the serum CA15-3 assay used to measure MUC1 in breast cancer to measure plasma MUC1 levels in ADTKD-MUC1. Read More

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Age-group-specific reference intervals for anti-Müllerian hormone and its diagnostic performance for polycystic ovary syndrome in a Korean population.

J Clin Lab Anal 2021 Jun 7:e23861. Epub 2021 Jun 7.

Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.

Background: We established age-group-specific reference intervals for serum anti-Müllerian hormone (AMH) levels in a Korean population and investigated the effectiveness of AMH assay for polycystic ovary syndrome (PCOS) diagnosis.

Methods: We analyzed serum levels of AMH, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) from 1540 Korean women. Subjects were divided into three groups: healthy, benign gynecologic diseases, and PCOS. Read More

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Venlafaxine and O-desmethylvenlafaxine serum levels are positively associated with antidepressant response in elder depressed out-patients.

World J Biol Psychiatry 2021 Jun 7:1-14. Epub 2021 Jun 7.

Department of Psychiatry, Bolzano, Italy.

Background: Therapeutic Drug Monitoring (TDM) represents one of the most promising tools in clinical practice to optimize antidepressant treatment. Nevertheless, little is still known regarding the relationship between clinical efficacy and serum concentration of venlafaxine (VEN). The aim of our study was to investigate the association between serum concentration of venlafaxine + O-desmethylvenlafaxine (SCVO) and antidepressant response (AR). Read More

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Lithium-Associated Hyperparathyroidism Followed by Catatonia.

AACE Clin Case Rep 2021 May-Jun;7(3):189-191. Epub 2020 Dec 19.

Valleywise Behavioral Health- Maryvale, 5102 W Campbell Ave, Phoenix, Arizona.

Objective: To familiarize the medical community with the less common adverse effects of lithium on parathyroid function, we present a case of lithium-associated hyperparathyroidism followed by the development of new-onset catatonia in a patient with schizoaffective disorder.

Methods: To allow for the safe resumption of lithium, the patient received laboratory screening of serum lithium, blood urea nitrogen, serum creatinine, calcium, and thyroid-stimulating hormone levels. The hypercalcemia was evaluated by measuring parathyroid hormone (PTH), ionized calcium, and 25-hydroxy vitamin D levels. Read More

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December 2020

Comparison of the Japan Society of Clinical Chemistry reference method and CDC method for HDL and LDL cholesterol measurements using fresh sera.

Pract Lab Med 2021 May 23;25:e00228. Epub 2021 Apr 23.

Department of Food and Nutritional Sciences, Practice Center for Registered Dietitian, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan.

Objectives: In 2009, the Japan Society of Clinical Chemistry (JSCC) recommended a reference method for the measurement of serum high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels. This automated method uses cholesterol esterase-cholesterol dehydrogenase to measure cholesterol levels in fractions obtained after ultracentrifugation and dextran sulfate/magnesium chloride precipitation. In the present study, using fresh samples, we compared the LDL-C and HDL-C levels measured using this method with those measured using the traditional Centers for Disease Control and Prevention (CDC)-beta-quantification (BQ) method. Read More

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Effects of endoscopic submucosal dissection on post-operative early treatment effectiveness and serum TAT-2 and GP73 expression levels in patients with early gastric cancer.

Exp Ther Med 2021 Aug 27;22(2):806. Epub 2021 May 27.

Department of Gastroenterology, The Eighth Affiliated Hospital of Guangxi Medical University, Guigang, Guangxi 537100, P.R. China.

The present study aimed to explore the effectiveness of endoscopic submucosal dissection (ESD) in the treatment of early gastric cancer (EGC) and its effect on serum tumor-associated trypsin-2 (TAT-2) and Golgi protein 73 (GP73) expression levels to provide a reference for the treatment of EGC. TAT-2 is a proteolytic target enzyme for tumor-associated trypsin inhibitor that has been previously reported to enhance invasion by promoting extracellular matrix degradation. GP73 is a novel type II Golgi membrane protein of unknown function that is expressed in the hepatocytes of patients with adult giant-cell hepatitis. Read More

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The Spatiotemporal Dynamics and Microevolution Events That Favored the Success of the Highly Clonal Multidrug-Resistant Monophasic Circulating in Europe.

Front Microbiol 2021 21;12:651124. Epub 2021 May 21.

Department of Bacteriology, Animal and Plant Health Agency, Addlestone, United Kingdom.

The European epidemic monophasic variant of enterica serovar (. 1,4,[5],12:i:-) characterized by the multi sequence type ST34 and the antimicrobial resistance ASSuT profile has become one of the most common serovars in Europe (EU) and the United States (US). In this study, we reconstructed the time-scaled phylogeny and evolution of this in Europe. Read More

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Is there an association between prostate-specific antigen and androgen levels in 46, XX patients with congenital adrenal hyperplasia?

J Pediatr Urol 2021 May 7. Epub 2021 May 7.

Multidisciplinary Clinic for Disorders of Sexual Development, Federal University of Bahia, Salvador, Bahia, Brazil. Electronic address:

Introduction: Congenital adrenal hyperplasia (CAH), a genetic disease characterized by defective cortisol synthesis and excessive levels of sex hormones, can cause precocious puberty in both sexes in untreated individuals and virilization in female patients with a 46, XX karyotype. The female paraurethral (Skene's) gland has been reported as prostate analogous. Growth of prostate tissue is associated with androgen production; therefore, prostate-specific antigen (PSA) levels may represent a marker of virilization in 46, XX patients with CAH. Read More

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Association of eosinophil-mediated inflammatory biomarkers with the presence of the Schatzki ring.

Adv Med Sci 2021 Jun 3;66(2):279-283. Epub 2021 Jun 3.

Department of Gastroenterology and Hepatology, Wroclaw Medical University, Wroclaw, Poland.

Purpose: The study aimed to assess the level of inflammatory biomarkers related to eosinophilia: interleukins 5 (IL-5) and 13 (IL-13), eotaxin 3, major basic protein (MBP) and transforming growth factor β1 (TGF-β1) in patients with dysphagia and Schatzki ring (SR), as well as the characteristics of this group of patients in terms of the features differentiating gastroesophageal reflux disease (GERD) and eosinophilic esophagitis (EoE).

Patients And Methods: We analyzed 42 patients with dysphagia, each of whom underwent panendoscopy with an assessment of the occurrence of SR, retrospectively assessed EoE Endoscopic Reference Score (EREFS) total, inflammatory and fibrostenotic and serum concentrations of IL-5 and 13, TGF-β1, eotaxin 3 and MBP. All of them completed a symptom and comorbid questionnaire. Read More

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Evaluation of liver function tests in the paediatric patient.

An Pediatr (Engl Ed) 2021 Jun 20;94(6):359-365. Epub 2021 May 20.

Unidad de Gastroenterología y Nutrición Pediátrica, Hospital Universitario Miguel Servet, Zaragoza, Spain.

Introduction: Although changes in liver function tests can be non-specific in numerous clinical conditions, they can be the first sign of a potentially serious disease in an asymptomatic patient.

Material And Methods: Retrospective cohort study, performed by reviewing the records of children of a reference hospital central laboratory with alanine aminotransferase enzyme (ALT) elevation during a 6-month aleatory period.

Results: 572 blood tests with serum ALT elevation corresponding to 403 patients have been assessed during the period studied. Read More

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Integrated metabolomics and proteomics analysis reveals energy metabolism disorders in the livers of sleep-deprived mice.

J Proteomics 2021 Jun 2;245:104290. Epub 2021 Jun 2.

Department of Child, Adolescent and Women's Health, School of Public Health, Capital Medical University, Beijing, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China. Electronic address:

Sleep deprivation (SD) has been linked to impaired mental and physical health, obesity, and various diseases. However, the molecular mechanism underlying the effects of SD in the liver is still unclear. To investigate the metabolome and proteome alterations in the liver, an in vivo model of SD was established based on automated random motion platform techniques by applying a strategy of 10 consecutive days of 20 h of sleep deprivation +4 h of resting. Read More

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Biomarkers for the Prediction and Diagnosis of Fibrostenosing Crohn's disease: A Systematic Review.

Clin Gastroenterol Hepatol 2021 Jun 2. Epub 2021 Jun 2.

Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA; Department of Gastroenterology, Hepatology, and Nutrition, Digestive Diseases and Surgery Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA.

Background And Aims: Intestinal strictures are a common complication of Crohn's disease (CD). Biomarkers of intestinal strictures would assist in their prediction, diagnosis, and monitoring. Herein we provide a comprehensive systematic review of studies assessing biomarkers that may predict or diagnose CD-associated strictures. Read More

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