32 results match your criteria reel novel

Using machine learning approaches for multi-omics data analysis: A review.

Biotechnol Adv 2021 Mar 29:107739. Epub 2021 Mar 29.

Division of Population Health and Genomics, School of Medicine, University of Dundee, Dundee, United Kingdom. Electronic address:

With the development of modern high-throughput omic measurement platforms, it has become essential for biomedical studies to undertake an integrative (combined) approach to fully utilise these data to gain insights into biological systems. Data from various omics sources such as genetics, proteomics, and metabolomics can be integrated to unravel the intricate working of systems biology using machine learning-based predictive algorithms. Machine learning methods offer novel techniques to integrate and analyse the various omics data enabling the discovery of new biomarkers. Read More

View Article and Full-Text PDF

Paperboard Coating Detection Based on Full-Stokes Imaging Polarimetry.

Sensors (Basel) 2020 Dec 31;21(1). Epub 2020 Dec 31.

Department of Design, Mid Sweden University, 831 25 Örnsköldsvik, Sweden.

The manufacturing of high-quality extruded low-density polyethylene (PE) paperboard intended for the food packaging industry relies on manual, intrusive, and destructive off-line inspection by the process operators to assess the overall quality and functionality of the product. Defects such as cracks, pinholes, and local thickness variations in the coating can occur at any location in the reel, affecting the sealable property of the product. To detect these defects locally, imaging systems must discriminate between the substrate and the coating. Read More

View Article and Full-Text PDF
December 2020

Compact and efficient O-band bismuth-doped phosphosilicate fiber amplifier for fiber-optic communications.

Sci Rep 2020 Jul 9;10(1):11347. Epub 2020 Jul 9.

G.G. Devyatyh Institute of Chemistry of High-Purity Substances of the Russian Academy of Sciences, Nizhny Novgorod, 603600, Russia.

During last decades there has been considerable interest in developing a fiber amplifier for the 1.3-[Formula: see text]m spectral region that is comparable in performance to the Er-doped fiber amplifier operating near 1.55 [Formula: see text]m. Read More

View Article and Full-Text PDF

Initial Public Health Response and Interim Clinical Guidance for the 2019 Novel Coronavirus Outbreak - United States, December 31, 2019-February 4, 2020.

MMWR Morb Mortal Wkly Rep 2020 Feb 7;69(5):140-146. Epub 2020 Feb 7.

Incident Manager, 2019-nCoV CDC Response, CDC.

On December 31, 2019, Chinese health officials reported a cluster of cases of acute respiratory illness in persons associated with the Hunan seafood and animal market in the city of Wuhan, Hubei Province, in central China. On January 7, 2020, Chinese health officials confirmed that a novel coronavirus (2019-nCoV) was associated with this initial cluster (1). As of February 4, 2020, a total of 20,471 confirmed cases, including 2,788 (13. Read More

View Article and Full-Text PDF
February 2020

Changes in microvascular oxygenation and total hemoglobin concentration of the vastus lateralis during neuromuscular electrical stimulation (NMES).

Physiother Theory Pract 2019 Aug 12:1-9. Epub 2019 Aug 12.

a Krannert School of Physical Therapy, University of Indianapolis , Indianapolis , IN , USA.

: Neuromuscular electrical stimulation (NMES) is predicated on eliciting muscle contractions and increasing muscle demand to promote increase in strength. Previous studies have shown differences in the magnitude of elicited force among various NMES waveforms but less is known about metabolic demand of muscle during NMES. : The purpose of this study was to compare elicited force and muscle metabolic demand during electrically elicited contractions using different NMES waveforms. Read More

View Article and Full-Text PDF

Modulation of TARP 8-Containing AMPA Receptors as a Novel Therapeutic Approach for Chronic Pain.

J Pharmacol Exp Ther 2019 06 25;369(3):345-363. Epub 2019 Mar 25.

Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana (K.L.K., R.M.A.S., W.G., B.L.A., K.M.G., D.L.G., P.L.O., W.P., J.R., C.D., H.W., Y.Q., K.D.B., A.N., V.B., S.S., J.C., J.M.W., K.-C.C., T.M.W., D.S., C.C.F., A.S.K., D.S.B., E.S.N.) and Lilly Research Centre, Eli Lilly and Company Ltd., Erl Wood Manor, Windlesham, Surrey, United Kingdom (R.Z., E.S.).

Nonselective glutamate -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor antagonists are efficacious in chronic pain but have significant tolerability issues, likely arising from the ubiquitous expression of AMPA receptors in the central nervous system (CNS). Recently, LY3130481 has been shown to selectively block AMPA receptors coassembled with the auxiliary protein, transmembrane AMPA receptor regulatory protein (TARP) 8, which is highly expressed in the hippocampus but also in pain pathways, including anterior cingulate (ACC) and somatosensory cortices and the spinal cord, suggesting that selective blockade of 8/AMPA receptors may suppress nociceptive signaling with fewer CNS side effects. The potency of LY3130481 on recombinant 8-containing AMPA receptors was modulated by coexpression with other TARPs; 2 subunits affected activity more than 3 subunits. Read More

View Article and Full-Text PDF

The research data management platform (RDMP): A novel, process driven, open-source tool for the management of longitudinal cohorts of clinical data.

Gigascience 2018 07;7(7)

Health Informatics Centre, University of Dundee, Mail Box 15, Ninewells Hospital & Medical School, Dundee, DD1 9SY, UK.

Background: The Health Informatics Centre at the University of Dundee provides a service to securely host clinical datasets and extract relevant data for anonymized cohorts to researchers to enable them to answer key research questions. As is common in research using routine healthcare data, the service was historically delivered using ad-hoc processes resulting in the slow provision of data whose provenance was often hidden to the researchers using it. This paper describes the development and evaluation of the Research Data Management Platform (RDMP): an open source tool to load, manage, clean, and curate longitudinal healthcare data for research and provide reproducible and updateable datasets for defined cohorts to researchers. Read More

View Article and Full-Text PDF

CRISPR Fish Reel in Novel Roles for Estrogen Receptors in Reproduction.

Endocrinology 2017 07;158(7):2082-2083

Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045.

View Article and Full-Text PDF

Electroencephalographic, cognitive, and neurochemical effects of LY3130481 (CERC-611), a selective antagonist of TARP-γ8-associated AMPA receptors.

Neuropharmacology 2017 Nov 28;126:257-270. Epub 2017 Jul 28.

Lilly Research Laboratories, Eli Lilly and Company, Windlesham, Surrey, UK. Electronic address:

6-[(1S)-1-[1-[5-(2-hydroxyethoxy)-2-pyridyl]pyrazol-3-yl]ethyl]-3H-1,3-benzothiazol-2-one (LY3130481 or CERC-611) is a selective antagonist of AMPA receptors containing transmembrane AMPA receptor regulatory protein (TARP) γ-8. This molecule has been characterized as a potent and efficacious anticonvulsant in an array of acute and chronic epilepsy models in rodents. The present set of experiments was designed to assess the effects of LY3130481 on the electroencephelogram (EEG), cognitive function, and neurochemical outflow. Read More

View Article and Full-Text PDF
November 2017

A semi-nested real-time PCR method to detect low chimerism percentage in small quantity of hematopoietic stem cell transplant DNA samples.

Genome 2017 Feb 14;60(2):183-192. Epub 2016 Oct 14.

b Medical Genetics, Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy.

Chimerism status evaluation of post-allogeneic hematopoietic stem cell transplantation samples is essential to predict post-transplant relapse. The most commonly used technique capable of detecting small increments of chimerism is quantitative real-time PCR. Although this method is already used in several laboratories, previously described protocols often lack sensitivity and the amount of the DNA required for each chimerism analysis is too high. Read More

View Article and Full-Text PDF
February 2017

Case Study: Johns Hopkins Community Health Partnership: A model for transformation.

Healthc (Amst) 2016 Dec 29;4(4):264-270. Epub 2016 Sep 29.

Johns Hopkins University School of Medicine, United States.

To address the challenging health care needs of the population served by an urban academic medical center, we developed the Johns Hopkins Community Health Partnership (J-CHiP), a novel care coordination program that provides services in homes, community clinics, acute care hospitals, emergency departments, and skilled nursing facilities. This case study describes a comprehensive program that includes: a community-based intervention using multidisciplinary care teams that work closely with the patient's primary care provider; an acute care intervention bundle with collaborative team-based care; and a skilled nursing facility intervention emphasizing standardized transitions and targeted use of care pathways. The program seeks to improve clinical care within and across settings, to address the non-clinical determinants of health, and to ultimately improve healthcare utilization and costs. Read More

View Article and Full-Text PDF
December 2016

Biomechanics of Fractures.

J Orthop Trauma 2016 Aug;30 Suppl 2:S2-6

Department of Orthopaedics, NYU Hospital for Joint Diseases, New York, NY.

Background: This video presents the digitized, original, reel-to-reel footage of Victor Frankel's groundbreaking 1960s experiments demonstrating the viscoelastic properties and fracture mechanics of loaded bone. As can be seen, novel instrumentation was used that resulted in an easily reproducible method of controlling bone loading rates. The innovation, and associated experiments, radically advanced our understanding of the mechanisms of acute fractures and bone's response to energy. Read More

View Article and Full-Text PDF

Reconstitution of synaptic Ion channels from rodent and human brain in Xenopus oocytes: a biochemical and electrophysiological characterization.

J Neurochem 2016 08 13;138(3):384-96. Epub 2016 Jun 13.

Lilly Research Centre, Eli Lilly and Company, Erl Wood Manor, Windlesham, Surrey, GU20 6HP, UK.

Disruption in the expression and function of synaptic proteins, and ion channels in particular, is critical in the pathophysiology of human neuropsychiatric and neurodegenerative diseases. However, very little is known regarding the functional and pharmacological properties of native synaptic human ion channels, and their potential changes in pathological conditions. Recently, an electrophysiological technique has been enabled for studying the functional and pharmacological properties of ion channels present in crude membrane preparation obtained from post-mortem frozen brains. Read More

View Article and Full-Text PDF

Zebrafish Reel in Phenotypic Suppressors of Autism.

Neuron 2016 Feb;89(4):673-5

Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 7610001, Israel. Electronic address:

Chemical genetics can help decipher novel pathways underlying neurodevelopmental psychiatric impairments. Hoffman et al. (2016) utilized behavioral profiling of psychoactive compounds in zebrafish and identified estrogens as suppressors of a phenotype resulting from loss of an autism risk gene. Read More

View Article and Full-Text PDF
February 2016

A Non-Reciprocal Autosomal Translocation 64,XX, t(4;10)(q21;p15) in an Arabian Mare with Repeated Early Embryonic Loss.

Reprod Domest Anim 2016 Feb 6;51(1):171-4. Epub 2015 Nov 6.

Texas A&M University, College Station, TX, USA.

Balanced autosomal translocations are a known cause for repeated early embryonic loss (REEL) in horses. In most cases, carriers of such translocations are phenotypically normal, but the chromosomal aberration negatively affects gametogenesis giving rise to both genetically balanced and unbalanced gametes. The latter, if involved in fertilization, result in REEL, whereas gametes with the balanced form of translocation will pass the defect into next generation. Read More

View Article and Full-Text PDF
February 2016

Ratchet-traction effect: an underdiagnosed mechanism of pacing lead dislodgement.

Indian Heart J 2014 Sep-Oct;66(5):555-6. Epub 2014 Jun 2.

Department of Cardiology, King George Medical University, Lucknow, U.P. 226003, India.

Pacing lead dislodgement contributes substantially, to the list of causes of early pacemaker failure. Reel's syndrome is a rare cause of pacemaker failure, resulting from the dislodging of pacing electrodes by manipulation of the pulse generator by the patient. We describe here an intriguing case of pacemaker lead dislodgement by a novel and frequently underdiagnosed mechanism that mimicked the fluoroscopic diagnosis of reel's syndrome. Read More

View Article and Full-Text PDF
September 2016

Novel intein-containing DNA specific primers for rapid identification of Candida glabrata using Real-Time PCR assays.

J Mycol Med 2014 Dec 2;24(4):337-40. Epub 2014 Oct 2.

Department of Microbiology, PRIST University, Thanjavur 614 904, Tamil Nadu, India. Electronic address:

Candida glabrata is an opportunistic human pathogen known to cause systemic and vaginal candidiasis. Rapid detection of Candida glabrata is indispensable for appropriate selection of antifungal drugs for chemotherapy. The study describes a unique intein-containing DNA fragment for specific detection of C. Read More

View Article and Full-Text PDF
December 2014

A first implementation of an automated reel-to-reel fluidic self-assembly machine.

Adv Mater 2014 Sep 27;26(34):5942-9. Epub 2014 Jun 27.

University of Minnesota, Electrical and Computer Engineering, Rm. 4-178, 200 Union St. SE, Minneapolis, MN, 55455, USA.

A first automated reel-to-reel fluidic selfassembly process for macroelectronic applications is reported. This system enables high-speed assembly of semiconductor dies (15 000 chips per hour using a 2.5 cm-wide web) over large-area substrates. Read More

View Article and Full-Text PDF
September 2014

Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA.

Elife 2014 Apr 29;3:e02190. Epub 2014 Apr 29.

Center for the Physics of Living Cells, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, United States Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Urbana, United States

Pif1 family helicases are conserved from bacteria to humans. Here, we report a novel DNA patrolling activity which may underlie Pif1's diverse functions: a Pif1 monomer preferentially anchors itself to a 3'-tailed DNA junction and periodically reel in the 3' tail with a step size of one nucleotide, extruding a loop. This periodic patrolling activity is used to unfold an intramolecular G-quadruplex (G4) structure on every encounter, and is sufficient to unwind RNA-DNA heteroduplex but not duplex DNA. Read More

View Article and Full-Text PDF

Changes in the distribution of atlantic bluefin tuna (Thunnus thynnus) in the Gulf of Maine 1979-2005.

PLoS One 2013 19;8(9):e75480. Epub 2013 Sep 19.

School of Marine Sciences, University of Maine, Orono, Maine, United States of America ; Gulf of Maine Research Institute, Portland, Maine, United States of America.

The Gulf of Maine, NW Atlantic Ocean, is a productive, seasonal foraging ground for Atlantic bluefin tuna (Thunnus thynnus), but commercial landings of adult size classes were up to 40% below the allocated total allowable catch between 2004 to 2008 for the rod and reel, harpoon, and purse seine categories in the Gulf of Maine. Reduction in Atlantic bluefin tuna catches in the Gulf of Maine could represent a decline in spawning stock biomass, but given wide-ranging, complex migration patterns, and high energetic requirements, an alternative hypothesis is that their dispersal patterns shifted to regions with higher prey abundance or profitability, reducing availability to U.S. Read More

View Article and Full-Text PDF

Increasing the efficiency of polymer solar cells by silicon nanowires.

Nanotechnology 2011 Aug 5;22(31):315401. Epub 2011 Jul 5.

Institute of Photonic Technology, Jena, Germany.

Silicon nanowires have been introduced into P3HT:[60]PCBM solar cells, resulting in hybrid organic/inorganic solar cells. A cell efficiency of 4.2% has been achieved, which is a relative improvement of 10% compared to a reference cell produced without nanowires. Read More

View Article and Full-Text PDF

A Novel Approach to Treating Anxiety and Enhancing Executive Skills in an Older Adult with Parkinson's Disease.

Clin Case Stud 2010 Feb;9(1):74-90

Rutgers the State University of New Jersey.

Scientific interest in the nonmotoric symptoms of Parkinson's disease (PD) has increased dramatically, and psychiatric symptoms (e.g., cognitive impairment, anxiety and mood disorders) are now considered prime targets for treatment optimization. Read More

View Article and Full-Text PDF
February 2010

Discovery of potent, selective, and orally active carboxylic acid based inhibitors of matrix metalloproteinase-13.

J Med Chem 2009 Jun;52(11):3523-38

Arthritis and Bone Metabolism Research, Novartis Institutes for BioMedical Research, Cambridge, MA 02139, USA.

The matrix metalloproteinase enzyme MMP-13 plays a key role in the degradation of type II collagen in cartilage and bone in osteoarthritis (OA). An effective MMP-13 inhibitor would therefore be a novel disease modifying therapy for the treatment of arthritis. Our efforts have resulted in the discovery of a series of carboxylic acid inhibitors of MMP-13 that do not significantly inhibit the related MMP-1 (collagenase-1) or tumor necrosis factor-alpha (TNF-alpha) converting enzyme (TACE). Read More

View Article and Full-Text PDF

Insights from a novel p53 isoform in zebrafish want to reel us in, but will we take the bait?

James J Manfredi

Genes Dev 2009 Feb;23(3):261-4

Department of Oncological Sciences, Mount Sinai School of Medicine, New York, New York 10029, USA.

The p53 tumor suppressor has been reported to be expressed as multiple isoforms. In this issue of Genes & Development, Chen and colleagues (pp. 278-290) use a zebrafish model to show that one such isoform, Delta113p53, is a target for transcriptional activation by p53 and can, in turn, inhibit the activity of the full-length p53 protein, establishing a novel negative feedback loop centered on the p53 locus itself. Read More

View Article and Full-Text PDF
February 2009

Flexible fluidic microchips based on thermoformed and locally modified thin polymer films.

Lab Chip 2008 Sep 24;8(9):1570-9. Epub 2008 Jul 24.

University of Twente, Institute for Biomedical Technology, Tissue Regeneration Group, Drienerlolaan 5, NL-7522, NB Enschede, The Netherlands.

This paper presents a fundamentally new approach for the manufacturing and the possible applications of lab on a chip devices, mainly in the form of disposable fluidic microchips for life sciences applications. The new technology approach is based on a novel microscale thermoforming of thin polymer films as core process. The flexibility not only of the semi-finished but partly also of the finished products in the form of film chips could enable future reel to reel processes in production but also in application. Read More

View Article and Full-Text PDF
September 2008

Discovery and SAR studies of novel GlyT1 inhibitors.

Bioorg Med Chem Lett 2007 Sep 30;17(18):5233-8. Epub 2007 Jun 30.

Lilly Research Laboratories, Eli Lilly and Company, Erl Wood Manor, Windlesham, Surrey GU20 6PH, UK.

Inhibition of the glycine transporter GlyT1 is a potential strategy for the treatment of schizophrenia. A novel series of GlyT1 inhibitors and their structure-activity relationships (SAR) are described. Members of this series are highly potent and selective transport inhibitors which are shown to elevate glycine levels in cerebrospinal fluid. Read More

View Article and Full-Text PDF
September 2007

Ambulatory methods for recording cough.

Jaclyn Smith

Pulm Pharmacol Ther 2007 7;20(4):313-8. Epub 2006 Nov 7.

Education and Research Centre, Academic Department of Medicine and Surgery, University of Manchester, Wythenshawe Hospital, Southmoor Rd, Wythenshawe, Manchester, M23 9LT, Manchester, UK.

Recording cough sounds to objectively quantify coughing was first performed using large reel-to-reel tape recorders more than 40 years ago. Coughs were counted manually, which is an extremely laborious and time-consuming process. Current technologies including digital recording techniques, data compression and improvements in digital storage capacity should make the process of recording and counting coughs suitable for automation; however, to date no accurate, objective cough monitoring device is available. Read More

View Article and Full-Text PDF

Molecular cloning and characterization of a novel human beta1,3-glucosyltransferase, which is localized at the endoplasmic reticulum and glucosylates O-linked fucosylglycan on thrombospondin type 1 repeat domain.

Glycobiology 2006 Dec 9;16(12):1194-206. Epub 2006 Aug 9.

Glycogene Function Team of Research Center for Glycoscience (RCG), National Institute of Advanced Industrial Science and Technology (AIST), Tukuba, Ibaraki, Japan.

Protein O-linked fucosylation is an unusual glycosylation associated with many important biological functions such as Notch signaling. Two fucosylation pathways synthesizing O-fucosylglycans have been reported on cystein-knotted proteins, that is, on epidermal growth factor-like (EGF-like) domains and on thrombospondin Type 1 repeat (TSR) domains. We report here the molecular cloning and characterization of a novel beta1,3-glucosyltransferase (beta3Glc-T) that synthesizes a Glcbeta1,3Fucalpha- structure on the TSR domain. Read More

View Article and Full-Text PDF
December 2006

CDB-4124 and its putative monodemethylated metabolite, CDB-4453, are potent antiprogestins with reduced antiglucocorticoid activity: in vitro comparison to mifepristone and CDB-2914.

Mol Cell Endocrinol 2002 Feb;188(1-2):111-23

Molecular Endocrinology Laboratory, BIOQUAL, Inc., 9600 Medical Center Drive, Rockville, MD 20850, USA.

To obtain selective antiprogestins, we have examined the in vitro antiprogestational/antiglucocorticoid properties of two novel compounds, CDB-4124 and the putative monodemethylated metabolite, CDB-4453, in transcription and receptor binding assays and compared them to CDB-2914 and mifepristone. All four antiprogestins bound with high affinity to rabbit uterine progestin receptors (PR) and recombinant human PR-A and PR-B (rhPR-A, rhPR-B) and were potent inhibitors of R5020-induced transactivation of the PRE2-tk-luciferase (PRE2-tk-LUC) reporter plasmid and endogenous alkaline phosphatase production in T47D-CO human breast cancer cells. None of these compounds exhibited agonist activity in these cells. Read More

View Article and Full-Text PDF
February 2002